CN115633696A - Use of tannic acid as an attractant component for Musca stabulans - Google Patents
Use of tannic acid as an attractant component for Musca stabulans Download PDFInfo
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- CN115633696A CN115633696A CN202211243480.5A CN202211243480A CN115633696A CN 115633696 A CN115633696 A CN 115633696A CN 202211243480 A CN202211243480 A CN 202211243480A CN 115633696 A CN115633696 A CN 115633696A
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- attractant
- tannic acid
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- stabulans
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- 239000005667 attractant Substances 0.000 title claims abstract description 53
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 45
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 239000001263 FEMA 3042 Substances 0.000 title claims abstract description 41
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 title claims abstract description 41
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 title claims abstract description 41
- 229940033123 tannic acid Drugs 0.000 title claims abstract description 41
- 235000015523 tannic acid Nutrition 0.000 title claims abstract description 41
- 229920002258 tannic acid Polymers 0.000 title claims abstract description 41
- 241000257229 Musca <genus> Species 0.000 title claims description 12
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 claims abstract description 19
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 claims abstract description 19
- 229930007744 linalool Natural products 0.000 claims abstract description 19
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 40
- 229960002446 octanoic acid Drugs 0.000 claims description 20
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 9
- 241000581981 Muscina stabulans Species 0.000 claims description 5
- 229920001864 tannin Polymers 0.000 claims description 4
- 235000018553 tannin Nutrition 0.000 claims description 4
- 239000001648 tannin Substances 0.000 claims description 4
- 241000233639 Pythium Species 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000581988 Muscina Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 28
- 239000000126 substance Substances 0.000 abstract description 12
- 239000002131 composite material Substances 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 6
- 241000255925 Diptera Species 0.000 description 12
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 241000282414 Homo sapiens Species 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000002917 insecticide Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 230000008786 sensory perception of smell Effects 0.000 description 2
- 208000004429 Bacillary Dysentery Diseases 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010017915 Gastroenteritis shigella Diseases 0.000 description 1
- 241000257226 Muscidae Species 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000196 olfactory nerve Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 201000005113 shigellosis Diseases 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an application of tannic acid as a component of a saprodontia stabbing fly attractant, simultaneously provides a plurality of composite attractants, simultaneously screens out tannic acid and linalool and the composite attractants with better luring effect on saprodontia stabbing flies, selects the optimal proportioning concentration of two compounds for preparation, lures the saprodontia stabbing flies through the coaction of the two substances, and achieves the optimal effect.
Description
Technical Field
The invention relates to application of tannic acid as a component of a Musca stabulans attractant, in particular to preparation of a fly attractant, belonging to the technical field of biology.
Background
Flies are one of important sanitary pests, are various in types, and not only disturb people and livestock, but also carry pathogen-transmitted diseases, including infectious diseases such as cholera, typhoid, bacillary dysentery, anthrax, pyococcus infection and the like. Some kinds of larvae can enter human bodies or livestock bodies to cause fly maggot diseases and harm the health of human and livestock. The activity area and the activity mode of the stable rotting flies are close to the life of human beings, and pathogens carried by the stable rotting flies are easily transmitted to the human beings, so the control of the stable rotting flies is particularly important, the fly control mainly depends on chemical control at present, although chemical insecticides have the advantages of quick effect, convenient use, suitability for large-scale application and the like, chemical components in the chemical insecticides can not only cause environmental pollution, but also can be unsafe or even poisoned to people and livestock, and the fly resistance to the insecticides can be easily caused. Therefore, the development direction is to find a simple, safe and environment-friendly fly prevention and control technology.
Research results show that odorous substances are important environmental information for flies to find partners, food and spawning sites. The odor substance in the airflow can cause olfactory nerve cells to generate electrophysiological reaction, and various odor substances have certain attraction effect on flies.
Disclosure of Invention
According to the invention, the environmental-friendly chemical substance combination which has an attraction effect on the Musca stabila is screened, the field effect evaluation of the attractant is carried out, the attractant formula combination which has an attraction effect on the Musca stabila is screened, and a comprehensive control way of the Musca stabila with the environmental-friendly attractant as a main body is explored.
In order to solve the technical problems, the invention adopts the technical scheme that:
firstly, the invention provides a new application of tannic acid as a component of the attractant for flies and is compounded with other components to obtain the attractant with better attraction effect.
The efficacy component of the fly attractant provided by the invention is a single component of tannic acid. A single component of tannic acid can be used as an attractant. Tannic acid is generally tannic acid having a chemical formula C 76 H 52 O 46 Is a tannin obtained from Galla chinensis, and is yellow or light brown light amorphous powder or flake; no smell and slight peculiar smellThe taste is extremely astringent, soluble in water and ethanol, easily soluble in glycerol, and hardly soluble in ether, chloroform or benzene.
To further verify the effect, the present invention was tested using the Muscina stabulans and provided an environmentally friendly fly attractant characterized in that the efficacy component was a tannin component.
Further, the environment-friendly fly attractant comprises a tannin component. The attractant is a solution containing the efficacy component, and the solvent of the solution is a volatile solvent. The solvent is ethanol or water.
Further, the concentration of the tannic acid is 0.01-100 mug/muL. The relationship between the concentration of tannic acid and the luring effect is obvious, and the tannic acid has good effect in the interval of 0.01-100 mug/mu L.
Furthermore, in order to select an attractant with a better preparation effect, the invention also selects and tests a plurality of composite attractants, and the invention selects linalool and caprylic acid to carry out a composite attractant test.
The use of linalool in a pythium stabilans attractant is not disclosed at present. The invention finds that linalool has very remarkable attraction effect on the stable flies,
caprylic acid is a known attractant ingredient in the art and has been reported in several patents for its use as an attractant for flies. China CN201110341476.8 reports a tyrosophila attractant, which contains an octanoic acid component. Because the variety of the flies is more, each fly has different sensitivity to different components, the invention also tests the attraction experiment of the caprylic acid to the stable rotting flies,
further, the invention provides an environment-friendly type pythium stablizer attractant which comprises one or 2 components of linalool and caprylic acid.
The invention has the beneficial effects that:
the application of the tannic acid as the component of the Musca stabulans attractant has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance rate, high attracting efficiency and the like, 2 kinds of compound attractants are provided, two kinds of compounds with good attracting effect on the Musca stabulans are screened out, the two kinds of compounds are printed with the optimal matching concentration for configuration, and the Musca stabulans are attracted through the combined action of the two kinds of substances, so that the optimal effect is achieved.
Detailed Description
The invention provides the use of tannic acid as a component of an attractant for flies, wherein the efficacy component of the attractant comprises tannic acid. The effective component refers to a component which plays an attraction effect, and is relative to substances such as auxiliary materials, solvents and other attraction components which do not interact with each other and have no attraction effect. Each component of the attractant can be conventional commercial products. The use comprises the following steps: use of tannic acid for the preparation of a fly attractant product, which product may be an attractant or an attractant means; the application of the tannic acid as the attractant for the flies can be realized by directly utilizing a single volatile matter of the tannic acid, can also be utilized in a composition form, and can also be utilized through a volatilization device. In short, various direct or indirect uses which only utilize the function of the attraction effect of tannic acid on flies belong to the technical scheme to be protected by the invention.
Example 1: single-component attraction effect test of tannic acid, linalool and caprylic acid
1. Preparation of attractant
The purchased linalool, caprylic acid and tannic acid powder is prepared with concentrations of 0.01 mug/mul, 0.1 mug/mul, 1 mug/mul and 10 mug/mul respectively by using absolute ethyl alcohol as a solvent, and is stored in a closed freezing storage tube after being fully mixed.
Tannic acid manufacturer: shanghai-sourced leaf Biotechnology, inc.; specification: s30456-250g.
2. Inducing experiment by Y-shaped olfactometer
Four experimental groups with concentrations of 0.01 mug/mul, 0.1 mug/mul, 1 mug/mul and 10 mug/mul and a blank control (absolute ethyl alcohol) group are respectively set up, placed in odor source emitting bottles of 5 olfaction instruments, placed in an olfaction instrument, and tested for various attractants and optimal concentrations.
3. Selecting the concentration with the best inducing effect
The results were: the best inducing effect of the octanoic acid is 12 octanoic acid at the concentration of 0.1 mug/mul (average value of three times of repetition)
The luring effect of linalool at the concentration of 10 mug/mul is the best, and the luring effect is 17 (the average value after three times of repetition)
The attracting effect of tannic acid is the best when the concentration of tannic acid is 1 mug/mul, and the attracting effect is 15 (the average value of tannic acid after three times of tannic acid repetition)
Example 2: composite attractant effect test
1. Preparation of attractant
The purchased linalool, caprylic acid and tannic acid powder uses absolute ethyl alcohol as a solvent, and a composite solution is prepared according to the following components:
octanoic acid + tannic acid, concentration of each component: octanoic acid 0.1 mug/muL, tannic acid 1 mug/muL.
The combination of linalool and tannic acid has the following concentration: linalool 10 mug/mug, tannic acid 1 mug/mug.
The combination of linalool, caprylic acid and tannic acid has the following concentration: 0.1 mug/mug L of caprylic acid, 10 mug/mug L of linalool and 1 mug/mug L of tannic acid.
The combination of caprylic acid and linalool has the following concentration: 0.1 mug/muL of caprylic acid and 10 mug/muL of linalool.
2. Inducing experiment by Y-shaped olfactometer
Four experimental groups and a blank control group (absolute ethyl alcohol) are respectively set up, the experimental groups and the blank control group are placed into odor source emitting bottles of 5 olfactometers, 25 Musca stabila are placed into the olfactometer, and various attractants and appropriate concentrations thereof are tested.
The results were:
the attracting effect of the linalool and the tannic acid is best as follows: 21 (average repeated three times).
Octanoic acid + tannic acid: 10 (average repeated three times).
Linalool + caprylic acid + tannic acid: 13 (average repeated three times).
Caprylic acid + linalool: 7 (average value repeated three times).
In effect, the attraction effect of a particular compound attractant is higher than that of a single attractant, and some compound attractants are not as effective as the single attractant, possibly due to the interaction between the attractants, influence on the volatilization of the components and change in odor.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and those skilled in the art should understand that they can make various changes, modifications, additions and substitutions within the spirit and scope of the present invention.
Claims (8)
1. Use of tannic acid as an attractant component for the Muscina stabulans.
2. The use as claimed in claim 1 wherein the efficacy component of the pythium stabolium attractant is a tannic acid single component.
3. An environmentally friendly Musca stabulans attractant characterized in that the efficacy component is a tannic acid component.
4. An environmentally friendly Muscina stabulans attractant as claimed in claim 3, comprising a tannic acid component.
5. An environmentally friendly Muscina stablizer attractant as set forth in claim 3 wherein said attractant is a solution containing said efficacy component, the solvent of said solution being a volatile solvent.
6. The environmentally friendly Muscina stabulans attractant of claim 5 wherein the solvent is ethanol or water.
7. The environmentally friendly Musca stabulans attractant of claim 5, wherein the tannin acid concentration is 0.01-100 μ g/μ L.
8. The environmentally friendly Muscina stabulans attractant of claim 3 further comprising one or 2 components selected from the group consisting of linalool and caprylic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211243480.5A CN115633696B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as attractant component for stable flies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211243480.5A CN115633696B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as attractant component for stable flies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115633696A true CN115633696A (en) | 2023-01-24 |
| CN115633696B CN115633696B (en) | 2024-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211243480.5A Active CN115633696B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as attractant component for stable flies |
Country Status (1)
| Country | Link |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100998433A (en) * | 2007-01-17 | 2007-07-18 | 北京市农林科学院 | Beverage contg. juice of cerasus humilis sok fruit and its preparing method |
| CN101096626A (en) * | 2006-06-30 | 2008-01-02 | 俞志勇 | Red bayberry wine and preparation method thereof |
| CN106258528A (en) * | 2016-08-09 | 2017-01-04 | 华南农业大学 | A kind of cultural method improving lemon mint volatile oil content |
| CN112300702A (en) * | 2020-11-10 | 2021-02-02 | 烟台大学 | Rusty protective coating with rust conversion function and preparation method thereof |
| CN112545955A (en) * | 2020-12-17 | 2021-03-26 | 广州环亚化妆品科技有限公司 | Anti-aging repair composition and preparation method and application thereof |
| CN113621342A (en) * | 2021-08-27 | 2021-11-09 | 四川大学 | Solvent-free binder and preparation method and application thereof |
| CN113930213A (en) * | 2021-09-27 | 2022-01-14 | 张云起 | Preparation method of water-resistant mosquito and fly sticking and catching net |
-
2022
- 2022-10-12 CN CN202211243480.5A patent/CN115633696B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096626A (en) * | 2006-06-30 | 2008-01-02 | 俞志勇 | Red bayberry wine and preparation method thereof |
| CN100998433A (en) * | 2007-01-17 | 2007-07-18 | 北京市农林科学院 | Beverage contg. juice of cerasus humilis sok fruit and its preparing method |
| CN106258528A (en) * | 2016-08-09 | 2017-01-04 | 华南农业大学 | A kind of cultural method improving lemon mint volatile oil content |
| CN112300702A (en) * | 2020-11-10 | 2021-02-02 | 烟台大学 | Rusty protective coating with rust conversion function and preparation method thereof |
| CN112545955A (en) * | 2020-12-17 | 2021-03-26 | 广州环亚化妆品科技有限公司 | Anti-aging repair composition and preparation method and application thereof |
| CN113621342A (en) * | 2021-08-27 | 2021-11-09 | 四川大学 | Solvent-free binder and preparation method and application thereof |
| CN113930213A (en) * | 2021-09-27 | 2022-01-14 | 张云起 | Preparation method of water-resistant mosquito and fly sticking and catching net |
Non-Patent Citations (3)
| Title |
|---|
| 闫丽英;刘星;张默函;刘雪萍;: "南美斑潜蝇寄主选择性研究进展", 内蒙古农业科技, no. 06 * |
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| CN115633696B (en) | 2024-03-29 |
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