CN115633691B - Use of tannins as mosquito attractant components - Google Patents
Use of tannins as mosquito attractant components Download PDFInfo
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- CN115633691B CN115633691B CN202211243479.2A CN202211243479A CN115633691B CN 115633691 B CN115633691 B CN 115633691B CN 202211243479 A CN202211243479 A CN 202211243479A CN 115633691 B CN115633691 B CN 115633691B
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- tannic acid
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- aedes albopictus
- attractant
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- 239000005667 attractant Substances 0.000 title claims abstract description 36
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 25
- 241000255925 Diptera Species 0.000 title claims description 24
- 229920001864 tannin Polymers 0.000 title description 7
- 239000001648 tannin Substances 0.000 title description 7
- 235000018553 tannin Nutrition 0.000 title description 7
- 229920002258 tannic acid Polymers 0.000 claims abstract description 36
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000001263 FEMA 3042 Substances 0.000 claims abstract description 35
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims abstract description 35
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 claims abstract description 35
- 229940033123 tannic acid Drugs 0.000 claims abstract description 35
- 235000015523 tannic acid Nutrition 0.000 claims abstract description 35
- 230000000694 effects Effects 0.000 abstract description 20
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 abstract description 18
- 241000256173 Aedes albopictus Species 0.000 abstract description 17
- KWKAKUADMBZCLK-UHFFFAOYSA-N 1-octene Chemical compound CCCCCCC=C KWKAKUADMBZCLK-UHFFFAOYSA-N 0.000 abstract description 9
- 239000002131 composite material Substances 0.000 abstract description 6
- 150000001875 compounds Chemical class 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 24
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 22
- 235000014655 lactic acid Nutrition 0.000 description 12
- 239000004310 lactic acid Substances 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- 229960002446 octanoic acid Drugs 0.000 description 11
- OBETXYAYXDNJHR-UHFFFAOYSA-N alpha-ethylcaproic acid Natural products CCCCC(CC)C(O)=O OBETXYAYXDNJHR-UHFFFAOYSA-N 0.000 description 8
- 239000002904 solvent Substances 0.000 description 6
- 239000011550 stock solution Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 239000000843 powder Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241001502567 Chikungunya virus Species 0.000 description 1
- 241000256059 Culex pipiens Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- TVMXDCGIABBOFY-UHFFFAOYSA-N n-Octanol Natural products CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of tannic acid as an aedes albopictus attractant component, which has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance, high attracting efficiency and the like, 2 composite attractants are provided, two compounds with good attracting effect on aedes albopictus are screened out, the two compounds are selected for configuration, and the optimal proportioning concentration of the two compounds is printed, so that the aedes albopictus is attracted through combined action of the two substances of the L-lactic acid and the octene, and the optimal effect is achieved.
Description
Technical Field
The invention relates to the field of biotechnology, in particular to preparation of a mosquito attractant, and in particular relates to application of tannic acid as a mosquito attractant component.
Background
Aedes albopictus, also called Asian tiger mosquito, is a common mosquito species in the south of Liaoning in China, is a strong-aggressiveness mosquito, is also an important disease vector organism, and can transmit various pathogens including viruses, chikungunya viruses and the like. Aedes albopictus is one of the 100 species with the fastest global diffusion rate in the past 20 years, and is one of the main mosquito species for urban mosquito killing.
The device has better attracting effect on blood-addicted mosquitoes, has good universality and is a practical device for monitoring blood-addicted mosquito population. However, the method does not consider the specificity of the emission of different hosts, so that the selective trapping of mosquitoes preferred by different hosts is difficult to realize, and the host preference of field mosquitoes is not reflected accurately. The main factor of attracting mosquito and sucking blood is the odor substances emitted by the host, and the difference of the types and the contents of the odor substances of the host is one of the important reasons for the preference of the mosquito host.
Disclosure of Invention
According to the invention, the environment-friendly chemical substance combination with the attracting effect on the aedes albopictus is screened indoors, the field effect evaluation of the attractants is carried out, the attractant formula combination with the attracting effect on the aedes albopictus is screened out, and the comprehensive prevention and treatment way of the aedes albopictus taking the environment-friendly attractants as the main body is explored.
In order to solve the technical problems, the invention adopts the following technical scheme:
first, the present invention provides the use of tannins as mosquito attractant components.
The efficacy component of the mosquito attractant provided by the invention is a single tannic acid component. The single component of tannic acid can be used as an attractant. Tannic acid generally refers to tannic acid, and has chemical formula of C76H52O46, and is a tannin obtained from Galla chinensis. Is yellow or light brown light amorphous powder or flake; odorless, slightly special smell, extremely astringent, soluble in water and ethanol, easily soluble in glycerol, and almost insoluble in diethyl ether, chloroform or benzene.
To further verify its effectiveness, the present invention was tested with Aedes albopictus and provides an environmentally friendly mosquito attractant characterized in that the efficacy component is a tannic acid component.
Further, the environment-friendly mosquito attractant comprises a tannic acid component. The attractant is a solution containing the efficacy component, and the solvent of the solution is a volatile solvent. The solvent is ethanol or water.
Further, the concentration of tannic acid is 0.01-100 mug/mug. The relation between the concentration of tannic acid and the attracting effect is obvious, the tannic acid has good effect in the interval of 0.01-100 mug/mug, and the effect is not obvious when the concentration is too low or too high.
Furthermore, in order to select the attractant with better preparation effect, a plurality of composite attractants are selected and tested, the octanoic acid and the lactic acid are selected for the composite attractant test, and a plurality of reports on the mosquito attractants of the octanoic acid and the lactic acid are known in the art.
The invention provides an environment-friendly aedes albopictus attractant which is characterized by comprising tannic acid and caprylic acid.
The invention provides an environment-friendly aedes albopictus attractant which is characterized by comprising tannic acid and lactic acid.
The invention has the beneficial effects that:
the application of the tannic acid as the aedes albopictus attractant component has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance, high attracting efficiency and the like, 2 composite attractants are provided, two compounds with good attracting effects on aedes albopictus are screened out, the two compounds are selected for configuration, and the optimal proportioning concentration of the two compounds is adopted, so that the aedes albopictus is attracted through the combined action of the two substances of the L-lactic acid and the octene, and the optimal effect is achieved.
Detailed Description
The present invention provides the use of tannic acid as a mosquito attractant component, wherein the efficacy component of the attractant comprises tannic acid. The efficacy component refers to a component which plays an attractive role, and is relative to auxiliary materials, solvents, other non-interactive attractive components and the like which have no attractive role. The components of the attractant can be conventional commercial products. The use comprises: use of tannic acid for the preparation of a mosquito attractant product, which may be an attractant or an attracting device; the tannins can be used as mosquito attractants, and can be directly used as single volatile matters of the tannins, can be used in the form of a composition, and can be used through a volatilizing device. In short, various direct or indirect uses of the function of attracting mosquitoes by the tannins belong to the technical scheme to be protected.
Example 1: single-component attracting effect test of tannic acid, octanoic acid and L-lactic acid
1. Preparation of attractants
The solution test effects of different concentrations are prepared by taking anhydrous ethanol as a solvent for the purchased octanoic acid stock solution, the L-lactic acid stock solution and the tannic acid powder, wherein the concentrations are respectively 0.01 mug/mu L, 0.1 mug/mu L, 1 mug/mu L and 10 mu g/mu L, and the octanoic acid stock solution, the L-lactic acid stock solution and the tannic acid powder are fully and uniformly mixed and then are stored in a closed freezing tube.
Tannic acid manufacturer: shanghai Yuan Ye Biotech Co., ltd; specification of: s30156-250 g.
2. Attracting experiment by Y-type olfactometer
Four experimental groups and blank control (absolute ethyl alcohol) groups with the concentration of 0.01 mu g/mu L, 0.1 mu g/mu L, 1 mu g/mu L and 10 mu g/mu L are respectively set up, the four experimental groups and blank control (absolute ethyl alcohol) groups are placed in odor source emission bottles of 5 smell instruments, 35 Aedes albopictus are placed in the smell instruments, and various attractants and proper concentrations thereof are tested.
3. Selecting the concentration of the L-lactic acid with the best attracting effect
The results were: the attracting effect of the octanoic acid at the concentration of 1 mug/mug is optimal and is 21 (average value of three times).
The attracting effect of the L-lactic acid at the concentration of 1 mug/mug is optimal, and the L-lactic acid is 11 (average value of three times).
The tannic acid has the best attracting effect at the concentration of 1 mug/mug, and the concentration is 18 (average value of three times). Wherein,
tannic acid is at 0.01 mu g/mu L, 0.1 mu g/mu L, and 10 mu g/mu L lure effect, is 10, 13, 16 respectively.
Example 2: composite attractant effect test
1. Preparation of attractants
The purchased L-lactic acid stock solution, 1-octene stock solution and tannic acid powder are prepared into a composite solution by using absolute ethyl alcohol as a solvent according to the following components:
octanoic acid + tannic acid combination, each component concentration: octene 0.1 mug/mug, tannic acid 1 mug/mug.
Lactic acid + tannic acid combination, each component concentration: lactic acid is 0.1 mug/mug, and tannic acid is 1 mug/mug.
Octene+lactic acid combination, concentration of each component: octene 0.1 mug/mug, lactic acid 1 mug/mug.
Lactic acid + caprylic acid + tannic acid, each component concentration: octene 0.1 [ mu ] g/[ mu ] L, lactic acid 0.1 [ mu ] g/[ mu ] L, tannic acid 1 [ mu ] g/[ mu ] L.
2. Attracting experiment by Y-type olfactometer
Four experimental groups and a blank control (absolute ethyl alcohol) group are respectively established, are put into odor source emission bottles of 5 olfactometers, 35 Aedes albopictus are put into the olfactometers, and various attractants and proper concentrations thereof are tested.
The results were:
the attracting effect of the octanoic acid and the tannic acid is best as follows: 28 (average of three replicates).
Lactic acid + tannic acid: 10 (average of three replicates).
Octene + lactic acid: 3 (average of three replicates).
Lactic acid + caprylic acid + tannic acid: 7 (average of three replicates).
It should be understood that the above description is not intended to limit the invention to the particular embodiments disclosed, but to limit the invention to the particular embodiments disclosed, and that various changes, modifications, additions and substitutions can be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (2)
1. Use of tannic acid as a mosquito attractant component.
2. The use according to claim 1, wherein the efficacy component of the mosquito attractant is a single component of tannic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211243479.2A CN115633691B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as mosquito attractant components |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211243479.2A CN115633691B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as mosquito attractant components |
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| CN115633691A CN115633691A (en) | 2023-01-24 |
| CN115633691B true CN115633691B (en) | 2024-03-29 |
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| CN202211243479.2A Active CN115633691B (en) | 2022-10-12 | 2022-10-12 | Use of tannins as mosquito attractant components |
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| CN116076511A (en) * | 2023-02-17 | 2023-05-09 | 山东农业大学 | Application of tannic acid in inducing plant to resist insects and preparation for inducing plant to resist insects |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1345586A (en) * | 2000-09-26 | 2002-04-24 | 肖景东 | Medicine for relieving itching and detumescence |
| CN101096626A (en) * | 2006-06-30 | 2008-01-02 | 俞志勇 | Red bayberry wine and preparation method thereof |
| CN112300702A (en) * | 2020-11-10 | 2021-02-02 | 烟台大学 | Rusty protective coating with rust conversion function and preparation method thereof |
| CN113863004A (en) * | 2021-09-28 | 2021-12-31 | 衣拉拉集团股份有限公司 | Mosquito-repelling antibacterial plant traditional Chinese medicine essential oil composition and preparation method of cotton knitted fabric with mosquito-repelling antibacterial function |
| CN115029925A (en) * | 2022-05-11 | 2022-09-09 | 江苏康溢臣生命科技有限公司 | Plant traditional Chinese medicine composition and preparation method of mosquito-repelling antibacterial mildew-proof cotton fiber thereof |
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- 2022-10-12 CN CN202211243479.2A patent/CN115633691B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1345586A (en) * | 2000-09-26 | 2002-04-24 | 肖景东 | Medicine for relieving itching and detumescence |
| CN101096626A (en) * | 2006-06-30 | 2008-01-02 | 俞志勇 | Red bayberry wine and preparation method thereof |
| CN112300702A (en) * | 2020-11-10 | 2021-02-02 | 烟台大学 | Rusty protective coating with rust conversion function and preparation method thereof |
| CN113863004A (en) * | 2021-09-28 | 2021-12-31 | 衣拉拉集团股份有限公司 | Mosquito-repelling antibacterial plant traditional Chinese medicine essential oil composition and preparation method of cotton knitted fabric with mosquito-repelling antibacterial function |
| CN115029925A (en) * | 2022-05-11 | 2022-09-09 | 江苏康溢臣生命科技有限公司 | Plant traditional Chinese medicine composition and preparation method of mosquito-repelling antibacterial mildew-proof cotton fiber thereof |
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