LU503751B1 - A harmonia axyridis attractant and preparation, preparation method and application thereof - Google Patents

A harmonia axyridis attractant and preparation, preparation method and application thereof Download PDF

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Publication number
LU503751B1
LU503751B1 LU503751A LU503751A LU503751B1 LU 503751 B1 LU503751 B1 LU 503751B1 LU 503751 A LU503751 A LU 503751A LU 503751 A LU503751 A LU 503751A LU 503751 B1 LU503751 B1 LU 503751B1
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LU
Luxembourg
Prior art keywords
attractant
preparation
parts
harmonia axyridis
diluent
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Application number
LU503751A
Other languages
French (fr)
Inventor
Qingxuan Xu
Xiaojun Guo
Su Wang
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Beijing Acad Agric & Forestry
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Publication of LU503751B1 publication Critical patent/LU503751B1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P19/00—Pest attractants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M2200/00—Kind of animal
    • A01M2200/01—Insects
    • A01M2200/012—Flying insects
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

A harmonia axyridis attractant and preparation, preparation method and application thereof relate to the field of biological control of pests. The attractant is prepared from the following raw materials in parts by weight: 5-8 parts of sclareol, 5-8 parts of terpilenol, 5-8 parts of eudesmol and 75-85 parts of nonanal. The attractant is prepared by mixing and stirring the above materials evenly. The attractant preparation consists of attractant and diluent with an attractant to diluent mass ratio of 1: (1-10). Attractant preparation is prepared by adding the attractant materials mentioned above directly to the diluent and mixing evenly. The attractant and the attractant preparation are applied in the preparation of drugs for attracting harmonia axyridis. The invention has the advantages that as the environmentally friendly control techniques, the attractant and its preparation are good attractants for harmonia axyridis, which are harmless to other natural predators.

Description

A HARMONIA AXYRIDIS ATTRACTANT AND PREPARATION, PREPARATION METHOD AND
LU503751
APPLICATION THEREOF
Technical Field
The invention relates to the field of biological control of pests, and in particular involves a harmonia axyridis attractant, its preparation, preparation method and application.
Background Technique
As special chemical signals, herbivore-induced plant volatiles (HIPVs) mediate plant-insect community interactions and affect insect behavior. HIPVs are mainly divided into terpene compounds, green leaf volatile (GLV), and substances containing nitrogen and sulfur. Terpene compounds are the most abundant plant volatiles, and also the most common compounds induced after pest damage. Among them, volatile substances such as monoterpenes and diterpenes are released after pest damage and directly participate in plant defense, such as attracting natural predator insects.
Harmonia axyridis belongs to Coleoptera and Coccidae. It is widely distributed in China and can effectively control pests such as woodlice and aphids. It is also the main predatory natural enemy in the agroforestry ecosystem.
In previous studies on the formulation of natural predator attractants, synomone is the main effective component, which is the plant volatiles induced by pests, and its effect is limited by the type and concentration ratio of substances. Therefore, further improvement is needed.
Contents of the Invention
The present invention is to disclose a harmonia axyridis attractant and its preparation.
The second aim of the present invention is to disclose the preparation method of the harmonia axyridis attractant and its preparation.
The third purpose of the present invention is to disclose the application of the attractant and the attractant preparation for the harmonia axyridis.
The purpose of the present invention is achieved through the following technical solutions:
A harmonia axyridis attractant is made up of the following raw materials in parts by weight: 5-8 parts of sclareol, 5-8 parts of terpilenol, 5-8 parts of eudesmol and 75-85 parts of nonanal.
In the harmonia axyridis attractant described in the above technical solution, the parts by weight of each raw material are 7.7 parts of sclareol, 7.7 parts of terpineol, 7.7 parts of eudesmol and 76.9 parts of nonanal.
In the preparation method of the attractant for harmonia axyridis described in the above technical solution, the weight parts of sclareol, terpineol, eudesmol, and nonanal are weighed and mixed evenly to obtain the attractant of harmonia axyridis. „7509757
In the harmonia axyridis attractant preparation, preparation is composed of an active ingredient which is the attractant described in the above technical solution and the diluent wherein the mass ratio of the active ingredient to the diluent is 1: (1-10).
In the attractant preparation described in the above technical solution, the mass ratio of the active ingredient to the diluent is 1: (5-10).
In the attractant preparation described in the technical solution above, the diluent is liquid paraffin.
In the preparation method of the attractant preparation described in the above technical solution, the attractant preparation is obtained by directly adding the weight parts of sclareol, terpineol, eudesmol and nonanal to the diluent and stirring evenly.
The attractant and the attractant preparation described in the above technical scheme are applied in the preparation of drugs for attracting harmonia axyridis.
The application described in the above technical solution refers to filling the attractant preparation into the slow-release materials before May to June, the summer peak of aphids in the orchard, and hanging it in the orchard.
The present invention has the following beneficial effects:
Pest-induced volatiles are generally volatile odorants released by plants in response to external stress after pest infestation. Some of these volatile substances will attract natural predator insects, which is a common system for the present research. In the process of studying this solution, Vitex negundo, a common native plant in northern China, was selected to extract the volatiles of Vitex negundo harmed by the cotton aphid. Therefore, as an environmentally friendly control technology, the attractant has a good attraction effect on the harmonia axyridis, and is harmless to other natural predators.
Brief Description of the Drawings
Fig. 1 shows the olfactory attraction effect of five different embodiments on harmonia axyridis.
Detailed Description
In order to make the technical solution of the present invention easier to understand, the following specific embodiments will further illustrate the harmonia axyridis attractant and preparation, preparation method and application thereof.
Embodiment 1: A kind of harmonia axyridis attractant
Weigh 7.7 mg of sclareol, 7.7 mg of terpineol, 7.7 mg of eudesmol, and 76.9 mg of nonanal, and mix and stir the above materials to obtain the attractant. 90875)
Embodiment 2: À kind of harmonia axyridis attractant
Weigh 5 mg of sclareol, 5 mg of terpineol, 8 mg of eudesmol, and 85 mg of nonanal, and mix and stir the above materials to obtain the attractant.
Embodiment 3: À kind of harmonia axyridis attractant
Weigh 8 mg of sclareol, 8 mg of terpineol, 5 mg of eudesmol, and 75 mg of nonanal, and mix and stir the above materials to obtain the attractant.
Embodiment 4: À kind of harmonia axyridis attractant
Weigh 5 mg of sclareol, 8 mg of terpineol, 7 mg of eudesmol, and 70 mg of nonanal, and mix and stir the above materials to obtain the attractant.
Embodiment 5: À kind of harmonia axyridis attractant preparation
Step 1. Weigh 7.7 mg of sclareol, 7.7 mg of terpineol, 7.7 mg of eudesmol, and 76.9 mg of nonanal;
Step 2. Weigh 100mg of liquid paraffin;
Step 3. Add the sclareol, terpineol, eudesmol, and nonanal in Step 1 directly to the liquid paraffin in Step 2, and stir evenly to obtain the attractant preparation.
Embodiment 6: A kind of harmonia axyridis attractant preparation
This embodiment is the same as Embodiment 5, except that the content of liquid paraffin is 200 mg.
Embodiment 7: A kind of harmonia axyridis attractant preparation
This embodiment is the same as Embodiment 5, except that the content of liquid paraffin is 500 mg.
Embodiment 8: A kind of harmonia axyridis attractant preparation
This embodiment is the same as Embodiment 5, except that the content of liquid paraffin is 1000 mg.
Embodiment 9: A kind of harmonia axyridis attractant preparation
This embodiment is the same as Embodiment 5, except that the content of liquid paraffin is 2000 mg.
The beneficial effects of the present invention will be described below through specific test examples.
Test Example: Olfactory Method
In the observation box of insect behavior, a Y-type olfactometer is used to test the selection preference of the adult harmonia axyridis for Embodiments 5 to 9.
LU503751
The composition and proportion of the mixed solute are sclareol: terpineol: eudesmol: nonanal=7.7: 7.7: 7.7: 76.9, and mixed solute: solvent=1:1-10, with liquid paraffin (paraffin oil) as the solvent.
The test used a Y-type insect olfactometer, with a 15 cm Y-tube main arm, and two 10 cm tube arms and connected it to a 250 mL Erlenmeyer flask. The atmospheric sampler sent air into the activated carbon tubes, passed through the long-neck distillation flask containing distilled water, and the air entered the olfactometer through the rubber tube, at the rate of 400 mL/min.
Dropped 10 ul solution onto a rectangular filter paper (2 cm x 1 cm) and placed it in the odorant bottle, and 10 pL of paraffin oil solution was added to the filter paper in another odorant bottle, and the filter paper was changed every hour. Before the test, the adult harmonia axyridis was starved for 24 hours, and a single adult harmonia axyridis was put into the Y-tube main arm to observe its behavior reaction. When the harmonia axyridis were at the center of the main arm, the timing started. When the harmonia axyridis crossed 1/2 of the two tube arms of the Y-type tube and stayed in this area for more than 5 seconds, it was recorded as making a selective response; when the harmonia axyridis had no obvious selection tendency within 5 minutes, it is recorded as no response. In the test, each harmonia axyridis was tested only once. Each tested harmonia axyridis needed to change the position of its two arms. Y-type olfactometer would be replaced after every five harmonia axyridis were tested. Each group would test 60 female and 60 male harmonia axyridis.
The chi-square test was used for the olfactory selection results of different tests by harmonia axyridis. First, the original data of the number of harmonia axyridis who selected the two arms were weighted, and then the chi-square test of the non-parametric test was used for comparative analysis.
The results of the olfactory test are shown in Fig. 1. It shows the olfactory attraction effect of embodiments 5 to 9 on the harmonia axyridis, wherein the horizontal axis represents the selection rate of the harmonia axyridis, namely the ratio of the left embodiment and the control paraffin oil.
Olfactory test results show that embodiment 5, 6, 7 and 8 have significant attracting effect on harmonia axyridis (x°=4.282, P=0.039; x°=9.309, P=0.002; x°=6.813, P=0.009; x°=4.654,
P=0.031), while embodiment 9 has no significant effect on harmonia axyridis (x?=0.835, P=0.361).
The above description is only a preferred embodiment of the invention and does not impose any formal and substantive restrictions on the invention. All technical personnel familiar with the profession, within the scope of the technical solution of the invention, make some changes, modifications and evolution equivalent changes when they can use the technical content 0375 disclosed above, which are equivalent embodiments of the invention; Meanwhile, any change, modification and evolution of the above embodiments based on the substantive technology of 5 the invention still fall within the scope of the technical solution of the invention.

Claims (9)

CLAIMS LU503751
1. A harmonia axyridis attractant, characterized in that the described attractant is made up of the following raw materials in parts by weight: 5-8 parts of sclareol, 5-8 parts of terpilenol, 5- 8 parts of eudesmol, and 75-85 parts of nonanal.
2. The harmonia axyridis attractant according to claim 1, characterized in that the parts by weight of each raw material are 7.7 parts of sclareol, 7.7 parts of terpineol, 7.7 parts of eudesmol and 76.9 parts of nonanal.
3. The preparation method of the harmonia axyridis attractantb according to claim 1 or 2, characterized in that the weight parts of sclareol, terpineol, eudesmol, and nonanal are weighed and mixed evenly to obtain the attractant of harmonia axyridis.
4. A harmonia axyridis attractant preparation, characterized in that the preparation is composed of an active ingredient which is the attractant described in claim 1 or claim 2 and the diluent wherein the mass ratio of the active ingredient to the diluent is 1: (1-10).
5. The attractant preparation according to claim 4, characterized in that the mass ratio of the active ingredient to the diluent is 1: (5-10).
6. The attractant preparation according to claim 4 or 5, characterized in that the diluent is liquid paraffin.
7. The preparation method of the attractant preparation according to any one from claim 4 to 6, characterized in that the attractant preparation is obtained by directly adding the weight parts of sclareol, terpineol, eudesmol and nonanal to the diluent and stirring evenly.
8. The attractant according to any of claims 1-2 and the attractant preparation according to any of claims 4-6 are applied in the preparation of drugs for attracting harmonia axyridis.
9. The application according to claim 8, characterized in that it refers to filling the attractant preparation into the slow-release materials before May to June, the summer peak of aphids in the orchard, and hanging it in the orchard.
LU503751A 2022-11-30 2023-03-27 A harmonia axyridis attractant and preparation, preparation method and application thereof LU503751B1 (en)

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Application Number Priority Date Filing Date Title
CN202211512977.2A CN115530165B (en) 2022-11-30 2022-11-30 Harmonia axyridis attractant, preparation thereof, preparation method and application

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LU503751B1 true LU503751B1 (en) 2023-09-28

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012280946B2 (en) * 2011-07-13 2015-10-08 Clarke Mosquito Control Products, Inc. Insecticidal compositions and methods of using the same
JP5810749B2 (en) * 2011-08-25 2015-11-11 住友化学株式会社 Harmful arthropod control composition and harmful arthropod control method
CN103271034B (en) * 2013-06-07 2014-04-30 福建农林大学 Cotesia plutellae kurdjumov attractant and preparation method thereof
JP6760945B2 (en) * 2015-08-31 2020-09-23 アース製薬株式会社 Flying pest attractant
CN110583645A (en) * 2019-09-16 2019-12-20 中国计量大学 Harmonia axyridis plant source attractant and application thereof
CN111781313B (en) * 2020-07-28 2022-09-09 山西省林业科学研究院 Method for obtaining walnut small Jiding plant source attractant raw material and formula thereof
CN112889832A (en) * 2021-01-21 2021-06-04 兰州大学 Application of composition containing cryptolepine analogue and small molecular terpene compounds in preventing and treating plant diseases
CN113892379B (en) * 2021-10-28 2023-01-31 信阳农林学院 Method for preventing and treating sooty mould of oil tea

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