CN112889832A - Application of composition containing cryptolepine analogue and small molecular terpene compounds in preventing and treating plant diseases - Google Patents
Application of composition containing cryptolepine analogue and small molecular terpene compounds in preventing and treating plant diseases Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
Abstract
The invention discloses application of a composition containing a cryptolepine analogue and a small molecular terpene compound in preventing and treating plant diseases. The effective components include picrinine, neopicrinine, sclareol, sclareolide, (+ -) -limonene, citronellol, ambergris ether, perillyl alcohol, perillaldehyde, 2-hexenal, 4-terpene alcohol, terpineol, alpha-terpineol, camphor, citral, geraniol, menthol, nerolidol, eucalyptol, curcumenol, cedrol and beta-ionone. The bactericidal composition can be used for preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast and phytophthora capsici. The components of the invention are all natural products, and the invention has the characteristics of low toxicity, low residue, environmental compatibility, difficult generation of drug resistance and the like, can cope with the occurrence of drug resistance of diseases, reduces the drug cost and is beneficial to the comprehensive management of the diseases.
Description
Technical Field
The invention belongs to the field of natural medicinal chemistry, and discloses application of a combination of a cryptolepine analogue and a micromolecular terpene compound in preventing and treating plant diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast and phytophthora capsici.
Background
China is a big agricultural country, and the yield of field crops is greatly lost due to the attack of plant diseases every year. Among the existing prevention and treatment schemes for plant diseases, chemical control methods are one of the most effective management strategies due to the advantages of fast response, low cost, easy operation and the like. However, the long-term use and abuse of existing traditional bactericides results in reduced resistance to pathogens and the development of resistant pathogens. Therefore, there is an urgent need to explore and develop new chemical entities with highly potent biological activity and unique mode of action as alternative fungicides to address the challenges presented by resistant pathogens.
Sinomenine (Cryptolepine) and neosinomenine (Neocryptolepine) are natural alkaloids with indoloquinoline as parent structure separated from root of Western nontraditional medicinal plant caulis seu folium Cayratiae Oligocarpae (Cryptolepis sanguinolentis), and have been reported to have pharmacological activities such as anticancer, anti-inflammatory, antimalarial, antituberculosis and antibacterial effects. In our earlier studies, it was found that: the cryptolepine and neocryptolepine have good application prospect in preventing and treating plant diseases due to excellent antibacterial activity. In consideration of the problems that the extraction cost, the synthesis difficulty and the like of the cryptolepine and neocryptolepine affect the practical application, the combination of natural alkaloid and other commercially available and low-price natural compounds is hoped to be found, so that the combination has the characteristics of reducing the cost, expanding the antibacterial spectrum, improving the activity and the like.
A large number of reports on antibacterial activity of natural source micromolecule terpene compounds are provided, and the compounds have the characteristics of simple structure, low market price, low toxicity and the like. A large number of experiments show that the application of the cryptolepine or the neocryptolepine and the natural source micromolecular terpene compounds in combination has a synergistic effect in the aspects of preventing and treating rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast, phytophthora capsici and the like. Therefore, the combination study of the natural terpene compound and the cryptolepine analogue for the antibacterial action has better application prospect.
Disclosure of Invention
The invention aims to provide a bactericide composition containing albophylline or neoalbophylline and a micromolecular terpene compound, which is used for preventing and treating plant diseases such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast, phytophthora capsici and the like.
In order to achieve the purpose, the invention provides the following technical method:
a bactericide composition containing Sinomenine or neosinomenine and small molecule terpene compounds comprises an active component A and an active component B, wherein the active component A is Sinomenine or neosinomenine, and the active component B is small molecule terpene compounds. The administration concentrations of the active components of the cryptolepine verticillium, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia grisea and phytophthora capsici are respectively 25 or 50, 0.5 or 1.0, 0.01, 0.025 or 0.05, 5 or 10, 25 or 50 mug/mL; the administration concentrations of the active components of the neobyssuline verticillium, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia grisea and phytophthora capsici are respectively 5, 5 or 10, 1, 2.5 or 5, 10 or 25 and 10 mu g/mL; the administration concentration of active component small molecular terpene compounds such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia oryzae and phytophthora capsici is 5, 25, 50, 100, 250 or 500 mu g/mL.
The bactericide composition provided by the invention has the following advantages:
1) the Sinomenine, neoSinomenine and small molecule terpene compounds are natural effective components. The bactericide has the characteristics of low toxicity, low residue, environmental compatibility, difficult generation of drug resistance and the like, and can be developed into green, pollution-free and nuisanceless botanical pesticides.
2) The bactericide composition provided by the invention shows obvious synergistic interaction effect in a certain proportion, and has the characteristic of improving bactericidal activity compared with a single agent.
3) The single-agent action mechanisms of the bactericidal composition provided by the invention are different, the composition is expected to delay or overcome the generation of disease drug resistance, the service life of the agent is prolonged, and the comprehensive treatment of diseases is facilitated.
Detailed Description
The foregoing invention is described in further detail by way of the following specific examples in order to provide a better understanding of the invention. This is not to be construed as limiting the invention. The experimental procedures described in the following examples are conventional unless otherwise specified.
Example 1: a bactericide composition containing Sinomenine or neoSinomenine and small molecule terpene compounds comprises an active component A and an active component B, wherein the active component A is Sinomenine or neosinomenine, and the active component B is small molecule terpene compounds.
Active component A
Active component B
Example 2: indoor activity determination of cryptolepine or combination of cryptolepine and sclareol on plant diseases
The plant pathogenic bacteria used in the experiment are strains preserved at 4 ℃ in a laboratory, and the adopted culture medium is a potato agar glucose culture medium (PDA for short). The PDA culture medium formula comprises: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.
The test method comprises the following steps: cleaning and peeling potatoes, weighing 200g of potatoes, cutting the potatoes into small pieces, adding water, boiling the potatoes thoroughly (boiling for 20-30 minutes and being capable of being punctured by a glass rod), filtering the potatoes by four layers of gauze, adding water into a beaker to 1000mL, adding 15-20g of agar according to experimental requirements, adding 20g of glucose, stirring the mixture to fully dissolve the agar, subpackaging the mixture in a triangular flask, and sterilizing the mixture for 20 minutes at 121 ℃ for later use. Dissolving single-dose compounds of cryptolepine, neocryptolepine and sclareol in DMSO respectively, adding into culture medium to certain concentration, mixing, and making into tablet with medicine. And (3) punching a circular fungus cake (with the diameter of 5mm) on the edge (the growth condition is consistent as much as possible) of hypha of each strain cultured for 3-6 days by using a puncher, then picking the hypha to the center of the cooled medicine-containing flat plate by using a inoculation needle, and placing the flat plate in a constant-temperature incubator at 25 ℃ for inverted culture. And (3) taking a culture medium containing an equivalent amount of DMSO as a control, taking the control hypha to grow over the culture dish as a limit, measuring the diameter of the hypha by adopting a cross method, and calculating the single-dose bacteriostasis rate of each compound. Adding the same concentration of Sinomenine or neosinomenine and sclareol into the culture medium, and determining the inhibition rate of the composition. All experiments were repeated three times. The calculation of the bacteriostasis rate is carried out according to the following calculation formula:
inhibition (%) × (control hypha diameter-treated hypha diameter)/(control hypha diameter-cake diameter) × 100
The compounding effect of the fungicide was evaluated according to the Abbott method, which uses the following formula:
％MIexp＝％MIa+％MIb–(％MIa×％MIb)
AI＝％MIobs/％MIexp
in the formula,% MIa and% MIb respectively represent the single-dose inhibition rate of the cryptolepine or neocryptolepine (A) and sclareol (B), and% MIexp and% MIobs respectively represent the expected value and the measured value of the mixed-dose inhibition rate. Evaluating the combination effect type of the mixture according to the synergistic coefficient (AI), namely the AI is less than or equal to 0.5 as antagonistic effect, the AI is more than 0.5 and less than 1.5 as additive effect, and the AI is more than or equal to 1.5 as synergistic effect.
TABLE 1 indoor bacteriostatic activity of combinations of cryptolepine or neocryptolepine and sclareol on plant diseases
As can be seen from the results of the activity test in Table 1, the combination of sinalbine and sclareol at a certain concentration has a synergistic effect on Pyricularia oryzae, and AI is 1.53. The combination was further tested for activity using different concentrations and the test data are shown in table 2.
TABLE 2 indoor bacteriostatic activity of combination of neobynine and sclareol at different concentrations on Pyricularia oryzae
As can be seen from the results of the activity tests in Table 2, the combinations of neographolide and sclareol at different concentrations showed additive and synergistic effects on Pyricularia oryzae.
Example 3: indoor activity determination of cynanchum wilfordii alkali or cynanchum wilfordii alkali and sclareolide combination on plant diseases
The test method was the same as in example 2, except that sclareolide was used instead of sclareol.
TABLE 3 indoor bacteriostatic activity of Sinomenine or neosinomenine and sclareol combinations on plant diseases
As can be seen from the results of the activity tests in Table 3, the combination of cryptolepine or neocryptolepine and sclareolide at a certain concentration exhibits additive effects on plant diseases such as Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Gibberella zeae, Pyricularia oryzae and Phytophthora capsici.
Example 4: indoor activity determination of combination of cryptolepine or neocryptolepine and (+/-) -limonene on plant diseases
The test method was the same as in example 2, except that (. + -.) -limonene was used instead of sclareol.
TABLE 4 indoor bacteriostatic activity of combinations of cryptolepine or neocryptolepine and (. + -.) -limonene on plant diseases
From the results of the activity tests in Table 4, it can be seen that the combination of cryptolepine or neocryptolepine and (+/-) -limonene mainly shows additive effects on plant diseases such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia grisea, phytophthora capsici and the like at a certain concentration.
Example 5: indoor activity determination of combination of bynine or neobynine and citronellol on plant diseases
The test method was the same as in example 2, except that citronellol was used instead of sclareol.
TABLE 5 indoor bacteriostatic activity of Sinomenine or Sinomenine and citronellol combinations on plant diseases
As can be seen from the results of the activity test in Table 5, the combination of bynine and citronellol at a certain concentration has a synergistic effect on Pyricularia oryzae, and AI is 1.51. The combination was further tested for activity using different concentrations and the test data are shown in table 6.
TABLE 6 indoor bacteriostatic activity of combination of cryptolepine and citronellol with different concentrations on Pyricularia oryzae
As can be seen from the results of the activity tests in Table 6, the combinations of various concentrations of bynine and citronellol showed additive and synergistic effects on Pyricularia oryzae.
Example 6: indoor activity assay of combination of Sinomenine or neosinomenine and ambergris ether on plant diseases
The test procedure was the same as in example 2, except that ambrox was used instead of sclareol.
TABLE 7 indoor bacteriostatic activity of Sinomenine or Neosinomenine and ambergris ether combinations on plant diseases
From the results of the activity tests in Table 7, it can be seen that the combination of cryptolepine or neocryptolepine and ambrox shows additive effects on plant diseases such as Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Gibberella graminearum, Magnaporthe grisea, Phytophthora capsici and the like at a certain concentration.
Example 7: indoor activity determination of cryptolepine or neocryptolepine and perillyl alcohol combination on plant diseases
The test procedure was the same as in example 2, except that sclareol was replaced by perillyl alcohol.
TABLE 8 indoor bacteriostatic activity of Sinomenine or neosinomenine and perillyl alcohol combination on plant diseases
From the activity test results in table 8, it can be seen that the combination of bynine and perillyl alcohol at a certain concentration has synergistic effect on rhizoctonia solani and botrytis cinerea, and AI is 1.51 and 1.90. The combination was further tested for activity using different concentrations and the test data are shown in table 9.
TABLE 9 combination of Sinomenine and perillyl alcohol at different concentrations for indoor bacteriostatic activity against Rhizoctonia solani and Botrytis cinerea
As can be seen from the results of the activity tests in Table 9, the combinations of bynine and citronellol at different concentrations showed additive and synergistic effects against Rhizoctonia solani and Botrytis cinerea.
Example 8: indoor activity determination of cynanchum wilfordii alkali or cynanchum wilfordii alkali and perillaldehyde combination on plant diseases
The test method was the same as in example 2, except that sclareol was replaced by perillaldehyde.
TABLE 10 indoor bacteriostatic activity of combinations of cryptolepine or neocryptolepine and perillaldehyde on plant diseases
From the activity test results in table 10, it can be seen that the combination of bynine and perillaldehyde at a certain concentration has a synergistic effect on botrytis cinerea, with an AI of 1.50. The combination was further tested for activity using different concentrations and the test data are shown in table 11.
TABLE 11 indoor bacteriostatic activity of various concentrations of the combination of Sinomenine and perillaldehyde on Botrytis cinerea
From the results of the activity tests in table 11, it can be seen that the combination of various concentrations of cryptomerine and perillaldehyde showed additive and synergistic effects on botrytis cinerea.
Example 9: indoor activity determination of combination of cryptolepine or neocryptolepine and 2-hexenal on plant diseases
The test method was the same as in example 2, except that 2-hexenal was used instead of sclareol.
TABLE 12 indoor bacteriostatic activity of combinations of Sinomenine or neosinomenine and 2-hexenal on plant diseases
From the results of the activity tests in table 12, it can be seen that the combination of bynine and 2-hexenal at a certain concentration has a synergistic effect on rhizoctonia solani with an AI of 1.69. The combination was further tested for activity using different concentrations and the test data are shown in table 11.
TABLE 13 indoor bacteriostatic activity of combination of cryptolepine and 2-hexenal at different concentrations on Rhizoctonia solani
As can be seen from the results of the activity tests in Table 13, the combination of various concentrations of cryptolepine and 2-hexenal showed additive and synergistic effects against Rhizoctonia solani.
Example 10: indoor activity determination of cynanchum wilfordii alkali or cynanchum wilfordii alkali and 4-terpene alcohol combination on plant diseases
The test procedure was the same as in example 2, except that 4-terpene alcohol was used in place of sclareol.
TABLE 14 indoor bacteriostatic activity of combinations of Sinomenine or neosinomenine and 4-terpene alcohol on plant diseases
As can be seen from the results of the activity tests in Table 14, the combination of bynine and 4-terpene alcohol at a certain concentration had a synergistic effect against Rhizoctonia solani with an AI of 1.76. The combination was further tested for activity using different concentrations and the test data are shown in table 15.
TABLE 15 indoor bacteriostatic activity of combinations of Sinomenine and 4-terpene alcohol at different concentrations in Rhizoctonia solani
As can be seen from the results of the activity tests in Table 15, the combinations of various concentrations of Sinomenine and 4-terpene alcohol showed additive and synergistic effects against Rhizoctonia solani.
Example 11: indoor activity determination of cryptolepine or neocryptolepine and terpineol combination on plant diseases
The test method was the same as in example 2, except that terpineol was used instead of sclareol.
TABLE 16 indoor bacteriostatic activity of cryptolepine or neocryptolepine and terpineol combinations on plant diseases
As can be seen from the results of the activity tests in Table 16, the combination of cryptolepine and terpineol at a certain concentration had a synergistic effect on Pyricularia oryzae, and AI was 1.56. The combination was further tested for activity using different concentrations and the test data are shown in table 17.
TABLE 17 indoor bacteriostatic activity of combinations of cryptolepine and terpineol of different concentrations on Pyricularia oryzae
As can be seen from the results of the activity tests in Table 17, the combinations of cryptolepine and terpineol at different concentrations exhibited additive and synergistic effects on Pyricularia oryzae.
Example 12: indoor activity determination of combination of cryptolepine or neocryptolepine and alpha-terpineol on plant diseases
The test procedure was the same as in example 2, except that α -terpineol was used instead of sclareol.
TABLE 18 indoor bacteriostatic activity of combinations of cryptolepine or neocryptolepine and alpha-terpineol on plant diseases
As can be seen from the results of the activity tests in Table 18, the combination of cryptolepine and alpha-terpineol at a certain concentration had a synergistic effect on Pyricularia oryzae, and AI was 1.56. The combination was further tested for activity using different concentrations and the test data are shown in table 19.
TABLE 19 indoor bacteriostatic activity of combinations of cryptolepine and alpha-terpineol at different concentrations on Pyricularia oryzae
As can be seen from the results of the activity tests in Table 19, the combinations of various concentrations of cryptolepine and alpha-terpineol showed additive and synergistic effects on Pyricularia oryzae.
Example 13: indoor activity determination of cryptolepine or neocryptolepine and camphor combination on plant diseases
The test method was the same as in example 2 except that camphor was used instead of sclareol.
TABLE 20 indoor bacteriostatic activity of combinations of cryptolepine or neocryptolepine and camphor on plant diseases
From the results of the activity tests in table 20, it can be seen that the combination of bynine and camphor at a certain concentration has synergistic effect on sclerotinia sclerotiorum and magnaporthe oryzae, and AI is 1.72 and 1.55. The combination was further tested for activity using different concentrations and the test data are shown in table 21.
TABLE 21 indoor bacteriostatic activity of combination of cryptolepine and camphor at different concentrations on sclerotinia sclerotiorum and magnaporthe oryzae
As can be seen from the results of the activity tests in Table 21, the combinations of cryptolepine and camphor at different concentrations showed synergistic effects on Sclerotinia sclerotiorum and additive and synergistic effects on Pyricularia oryzae.
Example 14: indoor activity determination of cryptolepine or neocryptolepine and citral combination on plant diseases
The test method was the same as in example 2, except that citral was used instead of sclareol.
TABLE 22 indoor bacteriostatic activity of cryptolepine or neocryptolepine and citral combination on plant diseases
The results of the activity tests in table 22 show that the combination of bynine and citral at a certain concentration has synergistic effect on rhizoctonia solani and phytophthora capsici, and AI is 1.59 and 1.51. The combination was further tested for activity using different concentrations and the test data are shown in table 23.
TABLE 23 indoor bacteriostatic activity of combinations of cryptolepine and citral at different concentrations on Rhizoctonia solani and Phytophthora capsici
As can be seen from the results of the activity tests in Table 23, the combinations of cryptolepine and citral at different concentrations showed additive and synergistic effects on Rhizoctonia solani and Phytophthora capsici.
Example 15: indoor activity assay of cynanchum wilfordii or cynanchum wilfordii and geraniol combination on plant diseases
The test method was the same as in example 2, except that geraniol was used instead of sclareol.
TABLE 24 indoor bacteriostatic activity of cryptolepine or neocryptolepine and geraniol combinations against plant diseases
The results of the activity tests in table 24 show that the combination of cryptolepine and geraniol at a certain concentration has synergistic effect on fusarium graminearum and pyricularia oryzae, and AI is 1.54 and 1.52. The combination was further tested for activity using different concentrations and the test data are shown in table 25.
TABLE 25 indoor bacteriostatic activity of combination of cryptolepine and geraniol in different concentrations on Gibberella zeae and Magnaporthe grisea
As can be seen from the results of the activity tests in Table 25, the combinations of cryptolepine and geraniol at different concentrations showed additive and synergistic effects on Fusarium graminearum and Magnaporthe grisea.
Example 16: indoor activity assay for plant diseases by using cryptolepine or neocryptolepine and menthol combination
The test method was the same as in example 2, except that menthol was used instead of sclareol.
TABLE 26 indoor bacteriostatic activity of cryptolepine or neocryptolepine and menthol combination on plant diseases
From the results of the activity tests in Table 26, it can be seen that cryptolepine or a combination of cryptolepine and menthol shows additive effects on Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Gibberella graminis, Magnaporthe grisea and Phytophthora capsici at a certain concentration.
Example 17: indoor activity determination of bigeline or combination of neobigeline and nerolidol on plant diseases
The test method was the same as in example 2, except that nerolidol was used instead of sclareol.
TABLE 27 indoor bacteriostatic activity of bigeline or neobigeline and nerolidol combination against plant diseases
From the results of the activity tests in Table 27, it can be seen that the combination of cryptolepine or neocryptolepine and nerolidol at a certain concentration exhibits additive effects on Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Gibberella graminearum, Magnaporthe grisea and Phytophthora capsici.
Example 18: indoor activity determination of cryptolepine or combination of neocryptolepine and eucalyptol on plant diseases
The test procedure was the same as in example 2, except that cineol was used instead of sclareol.
TABLE 28 indoor bacteriostatic activity of cryptolepine or neocryptolepine and eucalyptol combination on plant diseases
From the results of the activity tests in Table 28, it can be seen that the combination of cryptolepine or neocryptolepine and eucalyptol at a certain concentration exhibits additive effects on Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Gibberella graminearum, Pyricularia oryzae and Phytophthora capsici.
Example 19: indoor activity determination of cryptolepine or neocryptolepine and curcumenol combination on plant diseases
The test procedure was the same as in example 2 except that curcumenol was used instead of sclareol.
Indoor bacteriostatic activity of epi 29 cryptolepine or neocryptolepine and curcumenol combination on plant diseases
As can be seen from the results of the activity tests in Table 29, the combination of cryptolepine and curcumenol at a certain concentration has a synergistic effect on Sclerotinia sclerotiorum, and AI is 2.01. The combination was further tested for activity using different concentrations and the test data are shown in table 30.
TABLE 30 indoor bacteriostatic activity of various concentrations of the combination of cryptolepine and curcumenol on sclerotinia sclerotiorum
As can be seen from the results of the activity tests in Table 30, the combination of various concentrations of cryptolepine and curcumenol showed a synergistic effect on Sclerotinia sclerotiorum.
Example 20: indoor activity assay of combination of bynine or neobynine and cedrol on plant diseases
The test method was the same as in example 2, except that cedrol was used instead of sclareol.
TABLE 31 indoor bacteriostatic activity of cryptolepine or neocryptolepine and cedrol combination on plant diseases
From the results of the activity tests in table 31, it can be seen that a combination of cryptolepine or neocryptolepine and cedrol at a certain concentration exhibits additive effects against rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia grisea and phytophthora capsici example 21: indoor activity determination of bigeline or neobigeline and beta-ionone combination on plant diseases
The test procedure was the same as in example 2, except that beta-ionone was used instead of sclareol.
TABLE 32 indoor bacteriostatic activity of combinations of cryptolepine or neocryptolepine and beta-ionone on plant diseases
As can be seen from the results of the activity tests in Table 32, the combination of cryptolepine and beta-ionone at a certain concentration has synergistic effects on Rhizoctonia solani, Sclerotinia sclerotiorum and Pyricularia oryzae, AI is 1.50, 2.66 and 1.58; the combination of neobynine and beta-ionone has synergistic effect on Magnaporthe grisea with AI of 1.66. The combination was further tested for activity using different concentrations and the test data are shown in table 33.
TABLE 33 indoor bacteriostatic activity of various concentrations of grapevine or neograpevine in combination with beta-ionone against plant diseases
As can be seen from the results of the activity tests in Table 33, the combination of various concentrations of cryptolepine and beta-ionone showed additive and synergistic effects against Rhizoctonia solani, Sclerotinia sclerotiorum and Pyricularia oryzae; the combination of neobynine and beta-ionone with different concentrations shows additive and synergistic effects on rice blast germs.
In conclusion, when the cryptolepine analogue is used together with micromolecular terpenes, the cryptolepine analogue mainly shows additive and synergistic effects on plant diseases such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, rice blast, phytophthora capsici and the like. The invention is beneficial to the occurrence of disease drug resistance, and has certain practical value and further research value.
Claims (5)
1. The invention relates to application of a composition containing a cryptolepine analogue and a small molecular terpene compound in preventing and treating plant diseases.
2. The vitiligo analog of claim 1 is vitiligo, neovitiligo, and the small molecule terpene compound is sclareol, sclareolide, (±) -limonene, citronellol, ambergris ether, perillyl alcohol, perillaldehyde, 2-hexenal, 4-terpenol, terpineol, α -terpineol, camphor, citral, geraniol, menthol, nerolidol, eucalyptol, curcumenol, cedrol, β -ionone, and has the following molecular structure characteristics:
3. the use of the plant disease according to claim 1 which is caused by rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, pyricularia oryzae, and phytophthora capsici.
4. Use of the combination of Sinomenine and small molecule terpenoids according to claim 2 for the control of diseases caused by Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Gibberella tritici, Pyricularia oryzae and Phytophthora capsici.
5. Use of the novel combinations of Sinomenine and small molecule terpenoids according to claim 2 for the control of diseases caused by Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricularia oryzae and Phytophthora capsici.
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