CN101843245B - Culex and aedes attractant and application method - Google Patents
Culex and aedes attractant and application method Download PDFInfo
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- CN101843245B CN101843245B CN2010101987313A CN201010198731A CN101843245B CN 101843245 B CN101843245 B CN 101843245B CN 2010101987313 A CN2010101987313 A CN 2010101987313A CN 201010198731 A CN201010198731 A CN 201010198731A CN 101843245 B CN101843245 B CN 101843245B
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Abstract
The invention relates to culex and aedes attractant and an application method, belonging to the mosquito attractants containing organic compounds; the attractant in the invention is formed by mixing caprylic acid and benzpyrole, wherein the concentration of the caprylic acid is 10mug/ml, and the concentration of the benzpyrole is 0.1-1mug/ml, and the caprylic acid and the benzpyrole are prepared by 1:1 of volume ratio; the application method is that: the attractant is arranged in the room or at the field and is released slowly and naturally; in the field, the attractant is arranged in a glass long-neck volumetric flask or is released by being arranged in glass long-neck bottles with corks or is used with an insect trap. In the invention, the attractant can effectively attract culex pipiens pallens, aedes albopictus and other mosquitos, and the drug diffusion is little, and the attraction efficiency is high.
Description
Technical field
The invention belongs to the mosquito attractant that contains organic compound.
Background technology
Mosquito has Culex pipiens pallens, Culex bitaeniorhynchus, Culex quinquefasciatus, culex mimeticus, culex fuscanus, thin palpus culex, a palm fibre culex, Culex malayi, aedes albopictus etc.; wherein aedes albopictus and Culex pipiens pallens are distributed more widely in China, harm is larger; it is prevention and the important means of controlling mosquito matchmaker infectious disease that to protect mankind exempts from bite by mosquitos; particularly some be there is no to vaccine at present also without the mosquito matchmaker infectious disease of specific drug, repellent and lure killing mosquitoes to become the most feasible method.Development is rapid abroad recently for mosquito attractant, and the commodity mosquito attractants such as existing octonol, and the domestic commodity mosquito attractant that there is no, some scientific research departments start to carry out the screening of attractant compound, found that some are in the indoor compound that Culex pipiens pallens is had to attracting action, the theoretical foundation of seeking attractant or repellant.For example, the employings such as Zhang Shimiao " Y " type olfactometer has been measured acetone, ammoniacal liquor, decanedioic acid, positive enanthic acid, cyclohexane-carboxylic acid and 6 kinds of human skin volatiles of myristic acid to the luring and repellent action of the female adult of Culex pipiens pallens, finds that the positive enanthic acid of 0.1mg/L and 10mg/L acetone+10mg/L ammoniacal liquor have obvious attracting action (Zhang Shimiao, Mo Jianchu, Pan Chengyuan, Cheng Jiaan " several human skin volatiles are lured and repellent action the female adult of Culex pipiens pallens " insect knowledge 2004.41 (6) p545-547) to the female adult of Culex pipiens pallens.Ding Siyue etc. (2007) find 1mg/L and 10mg/L ammoniacal liquor, the positive enanthic acid of 1mg/L, 10mg/L and 100mg/L, and the 1mg/L octanol, 0.1mg/L and 1mg/L p-cresol have attracting action to Culex pipiens pallens.Above research there is no field attractant effect report.Before this, the present patent application people etc. utilize tentaculum electric potential technology and olfactometer to test electrophysiology and the behavioral implications of several odor compounds to the female mosquito of Culex pipiens pallens, result comprises that the female mosquito of Culex pipiens pallens has obvious tentaculum electric potential reaction to Pfansteihl, benzaldehyde and ammoniacal liquor, and the tentaculum electric potential reaction of benzaldehyde and ammoniacal liquor is proportionate; 1 μ L/mL Pfansteihl can produce maximum tentaculum electric potential reaction; 10 μ L/mL ammoniacal liquor have caused maximum tentaculum electric potential reaction.In the behavior impact, ammoniacal liquor has obvious sucking action to the female mosquito of Culex pipiens pallens when 10 μ L/mL; Benzaldehyde and aldehyde C-9 demonstrate potential repellent action, and both attraction rates are in 1 μ L/mL and 10 μ L/mL dosage 10% left and right only; Pfansteihl, benzoic acid, n-nonanoic acid and australene all do not produce obvious attracting action (Yu Jing, Li Chunxiao, Dong Yande, Zhao Tongyan " electrophysiology and the behavior reaction of Culex pipiens pallens to some compounds " " parasite and medical insect journal " 2008-15 (3) p154-157) to the female mosquito of Culex pipiens pallens under proof load.Yet, obtain a kind of efficiency of luring high, cheap, hypotoxicity, the compound attractant widely of drawing materials still needs to explore, and especially needs to adapt to the attractant that use is lured in field.
Sad and indoles is the compound (Yu Jing, " the sense of smell physiological Study of the bloodthirsty habit of Culex pipiens pallens " Military Medical Science Institute's doctorate paper, 2008) from detecting the volatile flavor material of chicken and mouse.
Summary of the invention
The present invention seeks to choose a kind of efficiency of luring high, cheap, hypotoxicity, the compound attractant widely of drawing materials, thereby provide to be applicable to indoor and field and to lure Culex pipiens pallens and aedes albopictus to produce the attractant of approach response, and propose corresponding compound combination preparation dosage and methods for using them.
The object of the invention realizes in the following manner:
At first the invention provides the attractant of culex and yellow-fever mosquito:
This attractant is mixed and is formed by sad and indoles, and wherein, sad concentration is 10 μ g/mL, and indoles concentration is 0.1～1 μ g/mL, both 1: 1 by volume mixed preparing.
Described attractant is during as indoor use, and its sad concentration is that 10 μ g/mL, indoles concentration are 0.1 μ g/mL.
When described attractant used as field, its sad concentration was that 10 μ g/mL, indoles concentration are 1 μ g/mL.
Described attractant is usingd ethanol as sad and solvent indoles.
Next the invention provides a kind of method of applying attractant:
This attractant is placed in indoor or field and naturally discharges.
When described attractant discharged in the wild, attractant was contained in glass long-neck volumetric flask or is contained in the glass flask of other tool plug and discharges.
Described attractant be will be equipped with the bottle of attractant be suspended on the insect attractant device other or be suspended on insect attractant device coverage scope within.
After the olfactometer contrast experiment of laboratory completed, the present invention had carried out series of experiments and has obtained statistical data comparatively accurately in indoor and field, thereby showed that good effect and progress that the present invention has are:
The invention provides can be used to luring the Chemical composition that of comprising of culex and yellow-fever mosquito of sad and indoles, and proposed compound concentration and the using dosage of said composition.
The present invention not only can lure Culex pipiens pallens and aedes albopictus effectively, and affect according to odor compound the pattern that mosquito tends to mechanism and assembles behavior, also can lure the multiple mosquitoes such as Culex bitaeniorhynchus, Culex quinquefasciatus, culex mimeticus, culex fuscanus, thin palpus culex, a palm fibre culex, Culex malayi.
The present invention lures the diffusion that its concentrated poisoning can be reduced after mosquito to chemical agent.
Particularly, when the present invention uses in the wild, can obviously improve and use merely CO
2The attractant effect of mosquito luring lamp.If kill and will greatly reduce the mosquito population quantity at the initial stage that mosquito occurs, luring the adult mosquito union, minimizing man dwells, and mosquito harasses and thorn is stung probability, will become the more efficiently method of prevention and control mosquito matchmaker infectious disease.
The present invention will be further described by the following examples, and embodiment comprises but do not limit the scope of the invention.
Embodiment
Embodiment 1
In laboratory, using sad 10 μ g/mL, indoles 0.1 μ L/mL mixing in 1: 1 by volume as attractant.With T-shape olfactometer (with reference to Geier et al., 1996 design has also been done some and revised), measure the attracting action of attractant to the female adult of Culex pipiens pallens.T-shape olfactometer base brachium 35cm, two each long 20cm of " T " arm, diameter 7cm.Basic arm is connected to the mosquito release room; Article two, " T " arm end extends 2 sample rooms, and air-flow is connected with sample room by " Y " pipe.Before air-flow entered sample room, air was first through activated carbon filtration and distilled water humidification, and the gas flow of regulating in olfactometer is 900ml/min.26～28 ℃ of room temperatures, relative moisture 70～80%, the luminous intensity in olfactometer is 100～150lux.With mosquito sucking tube, draw 30 female mosquitos and insert people mosquito release room, after mosquito adapts to 15 minutes, (5cm * 5cm) put at random left and right sample room with the identical filter paper (in contrast) that is soaked with the 0.5mL solvent, after timing 20min, record enters the mosquito number of processing in arm and control arm for the filter paper that tries attractant solution will to be soaked with respectively 0.5mL.
Each sample repeats 6 times, tests 30 female mosquitos at every turn, and all transposing is processed and control site at every turn, may be on the impact of left arm or right arm trend to eliminate mosquito.
Result: attractant arm end can attract approximately 85% mosquito.The female mosquito of Culex pipiens pallens produces the approach behavior reaction to attractant, and attractant has obvious sucking action to the female mosquito of Culex pipiens pallens.
Embodiment 2:
In laboratory, usining sad 10 μ g/mL, indoles 0.1 μ L/mL mixes as attractant by 1: 1 volume ratio.With the T-shape olfactometer, measure the attracting action of compound to the female adult of aedes albopictus.Method of testing is the same.
Result: attractant arm end can attract approximately 88% mosquito.The female mosquito of aedes albopictus produces the approach behavior reaction to attractant, and attractant has obvious sucking action to the female mosquito of aedes albopictus.
Embodiment 3:
The field release test.This experiment is carried out according to around people room, village in Xun Dian county, Kunming I in August, 2009.Adopt microorganism epidemic research institute of the Chinese military medicine academy of sciences to combine the mosquito trapper MT-1 (GB/T5836.2) of manufacture with Beijing grand hat development in science and technology Co., Ltd, trapper, apart from about the 1.5m of ground, is distinguished interval more than 50 meters, trapper CO
2Air-flow is blown into mosquito in bag by fan.Sad, indoles are made into respectively to 10 μ g/mL and 1 μ L/mL concentration with ethanol, and the vial of packing into after mixing in 1: 1 by volume, be suspended on by mosquito luring lamp.Trapping from afternoon 5:00 to 10:00 in evening.Adopt attractant+CO
2Mosquito luring lamp and CO
2Mosquito luring lamp contrasts simultaneously, tested altogether 3 days, by 3 days lure the mosquito result average, result such as following table:
In upper table, using without the trapping mosquito number of attractant as the trapping base value, the catching mosquito number of attractant and this base value are compared, the mosquito number that attractant is caught is more 20.6% than this base value, proves that attractant has obvious enhancing attractant effect.
Embodiment 4:
The field release test.By attractant+CO
2Mosquito luring lamp and lactic acid+CO
2Mosquito luring lamp compares, and all the other are identical with embodiment 3 conditions.Trapping since afternoon 4:30 simultaneously to evening 10:30 finish, tested altogether 3 days, by 3 days lure the mosquito result average.
Result: attractant+CO
2The mosquito number that mosquito luring lamp is caught is more 16.1% than contrast, shows that the attractant effect is better than lactic acid.
Claims (5)
1. the attractant of culex and yellow-fever mosquito, is characterized in that this attractant is mixed and formed by sad and indoles, and wherein, sad concentration is 10 μ g/mL, and indoles concentration is 0.1～1 μ g/mL, and both are the 1:1 mixed preparing by volume;
Described attractant is during as indoor attractant, and its sad concentration is that 10 μ g/mL, indoles concentration are 0.1 μ g/mL;
During attractant that described attractant is used as field, its sad concentration is that 10 μ g/mL, indoles concentration are 1 μ g/mL;
Described attractant is usingd ethanol as sad and solvent indoles.
2. the method for an application attractant as claimed in claim 1, is characterized in that attractant is placed in to indoor or field to be discharged naturally.
3. application attractant method according to claim 2, while it is characterized in that field discharges, attractant is contained in glass long-neck volumetric flask or is contained in the glass flask of other tool plug and discharges.
4. according to claim 2 or 3 described application attractant methods, within the bottle that it is characterized in that being equipped with attractant is suspended on insect attractant device coverage scope.
5. according to claim 2 or 3 described application attractant methods, while is characterized in that discharging in the wild, further by trapper CO
2Air-flow is blown into mosquito in bag by fan, and sad, indoles are made into respectively to 10 μ g/mL and 1 μ L/mL concentration with ethanol, and the vial of packing into after 1:1 mixing by volume, be suspended on by mosquito luring lamp.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101987313A CN101843245B (en) | 2010-06-12 | 2010-06-12 | Culex and aedes attractant and application method |
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| CN2010101987313A CN101843245B (en) | 2010-06-12 | 2010-06-12 | Culex and aedes attractant and application method |
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| CN101843245B true CN101843245B (en) | 2013-11-20 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125037A (en) * | 2011-01-12 | 2011-07-20 | 马晓光 | Mosquito trapping liquid |
| CN107114412A (en) * | 2017-05-22 | 2017-09-01 | 和县乌江镇雅轩家庭农场 | A kind of flies lure agent |
| WO2019028585A1 (en) * | 2017-08-07 | 2019-02-14 | 广州威佰昆生物科技有限公司 | Aedes albopictus and production method therefor |
| CN113367141A (en) * | 2021-05-14 | 2021-09-10 | 深圳市蓝禾技术有限公司 | Mosquito attractant and application thereof |
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2010
- 2010-06-12 CN CN2010101987313A patent/CN101843245B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 余静.淡色库蚊嗜血习性的嗅觉生理研究.《军事医学科学院博士学位论文》.2008,实验结果部分，具体参见第51-52页表12. |
| 淡色库蚊嗜血习性的嗅觉生理研究;余静;《军事医学科学院博士学位论文》;20081130;实验结果部分，具体参见第51-52页表12 * |
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