CN101843245A - Culex and aedes attractant and application method - Google Patents
Culex and aedes attractant and application method Download PDFInfo
- Publication number
- CN101843245A CN101843245A CN201010198731A CN201010198731A CN101843245A CN 101843245 A CN101843245 A CN 101843245A CN 201010198731 A CN201010198731 A CN 201010198731A CN 201010198731 A CN201010198731 A CN 201010198731A CN 101843245 A CN101843245 A CN 101843245A
- Authority
- CN
- China
- Prior art keywords
- attractant
- mosquito
- culex
- concentration
- sad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to culex and aedes attractant and an application method, belonging to the mosquito attractants containing organic compounds; the attractant in the invention is formed by mixing caprylic acid and benzpyrole, wherein the concentration of the caprylic acid is 10mug/ml, and the concentration of the benzpyrole is 0.1-1mug/ml, and the caprylic acid and the benzpyrole are prepared by 1:1 of volume ratio; the application method is that: the attractant is arranged in the room or at the field and is released slowly and naturally; in the field, the attractant is arranged in a glass long-neck volumetric flask or is released by being arranged in glass long-neck bottles with corks or is used with an insect trap. In the invention, the attractant can effectively attract culex pipiens pallens, aedes albopictus and other mosquitos, and the drug diffusion is little, and the attraction efficiency is high.
Description
Technical field
The invention belongs to the mosquito attractant that contains organic compound.
Background technology
Mosquito has Culex pipiens pallens, Culex bitaeniorhynchus, Culex quinquefasciatus, culex mimeticus, culex fuscanus, thin palpus culex, a palm fibre culex, Culex malayi, aedes albopictus etc.; wherein aedes albopictus and Culex pipiens pallens are distributed more widely in China, harm is bigger; the human bite by mosquitos that exempts from of protection is prevention and the important means of controlling mosquito matchmaker infectious disease; particularly some still there is not the mosquito matchmaker infectious disease that vaccine does not have specific drug yet at present, repellent and lure killing mosquitoes to become the most feasible method.Development is rapid abroad recently for the mosquito attractant, and commodity mosquito attractants such as existing octonol, and domesticly still there is not a commodity mosquito attractant, some scientific research departments begin to carry out the screening of attractant compound, found that some have the compound of attracting action, the theoretical foundation of seeking attractant or repellant indoor to Culex pipiens pallens.For example, employings such as Zhang Shimiao " Y " type olfactometer has been measured acetone, ammoniacal liquor, decanedioic acid, positive enanthic acid, cyclohexane-carboxylic acid and 6 kinds of human scent materials of myristic acid to the luring and repellent action of Culex pipiens pallens female adult worm, finds that the positive enanthic acid of 0.1mg/L and 10mg/L acetone+10mg/L ammoniacal liquor have tangible attracting action (Zhang Shimiao, Mo Jianchu, Pan Chengyuan, Cheng Jiaan " several human scent materials are lured and repellent action the Culex pipiens pallens female adult worm " insect knowledge 2004.41 (6) p545-547) to the Culex pipiens pallens female adult worm.Ding Siyue etc. (2007) find 1mg/L and 10mg/L ammoniacal liquor, the positive enanthic acid of 1mg/L, 10mg/L and 100mg/L, and the 1mg/L octanol, 0.1mg/L and 1mg/L p-cresol have attracting action to Culex pipiens pallens.More than research does not still have open-air attractant effect report.Before this, the present patent application people etc. utilize tentaculum electric potential technology and olfactometer to test feeler electricity physiology and the behavioral implications of several odor compounds to the female mosquito of Culex pipiens pallens, the result comprises that the female mosquito of Culex pipiens pallens has tangible tentaculum electric potential reaction to L-lactic acid, benzaldehyde and ammoniacal liquor, and the tentaculum electric potential reaction of benzaldehyde and ammoniacal liquor is proportionate; 1 μ L/mL L-lactic acid can produce maximum tentaculum electric potential reaction; 10 μ L/mL ammoniacal liquor have caused maximum tentaculum electric potential reaction.In the behavior influence, ammoniacal liquor has tangible sucking action to the female mosquito of Culex pipiens pallens when 10 μ L/mL; Benzaldehyde and aldehyde C-9 demonstrate potential repellent action, and both attraction rates are at 1 μ L/mL and 10 μ L/mL dosage only about 10%; L-lactic acid, benzoic acid, n-nonanoic acid and australene all do not produce tangible attracting action (Yu Jing, Li Chunxiao, Dong Yande, Zhao Tongyan " Culex pipiens pallens is to the feeler electricity physiology and the behavior reaction of some compounds " " parasite and medical insect journal " 2008-15 (3) p154-157) to the female mosquito of Culex pipiens pallens under proof load.Yet, obtain a kind of efficient height of luring, cheap, hypotoxicity, the compound attractant of drawing materials widely still need to explore, and especially need to adapt to the attractant that use is lured in the field.
Sad and indoles is the compound (Yu Jing, " the sense of smell physiological Study of the bloodthirsty habit of Culex pipiens pallens " Military Medical Science Institute's doctorate paper, 2008) that detects from the volatile flavor material of chicken and mouse.
Summary of the invention
The present invention seeks to choose a kind of efficient height of luring, cheap, hypotoxicity, the compound attractant widely of drawing materials, be applicable to indoor and open-air attractant of luring Culex pipiens pallens and aedes albopictus to produce approach response thereby provide, and propose corresponding compounds formulated in combination dosage and methods for using them.
The object of the invention realizes in the following manner:
At first the invention provides the attractant of culex and yellow-fever mosquito:
This attractant is mixed by sad and indoles and forms, and wherein, sad concentration is 10 μ g/mL, and indoles concentration is 0.1～1 μ g/mL, both 1: 1 by volume mixed preparing.
Described attractant is during as indoor use, and its sad concentration is that 10 μ g/mL, indoles concentration are 0.1 μ g/mL.
When described attractant used as the field, its sad concentration was that 10 μ g/mL, indoles concentration are 1 μ g/mL.
Described attractant with ethanol as sad and solvent indoles.
Next the invention provides a kind of method of using attractant:
This attractant places indoor or open-air dispensing naturally.
When described attractant discharged in the open air, attractant was contained in the glass long-neck volumetric flask or is contained in the interior dispensing of glass flask of other tool plug.
Described attractant be will be equipped with the bottle of attractant to be suspended on the insect attractant device other or be suspended within the insect attractant device coverage scope.
After the olfactometer contrast experiment of laboratory finished, the present invention had carried out a series of experiments and has obtained statistical data comparatively accurately in indoor and field, thereby showed that good effect and progress that the present invention has are:
The invention provides the Chemical composition that can be used to lure comprising of culex and yellow-fever mosquito of sad and indoles, and proposed the compound concentration and the using dosage of said composition.
The present invention not only can lure Culex pipiens pallens and aedes albopictus effectively, and according to the pattern that odor compound influences mosquito trend mechanism and assembles behavior, also can lure Culex bitaeniorhynchus, Culex quinquefasciatus, culex mimeticus, culex fuscanus, carefully must culex, multiple mosquitoes such as a palm fibre culex, Culex malayi.
The present invention lures the diffusion that its concentrated poisoning can be reduced behind the mosquito chemical agent.
Particularly, when the present invention uses in the open air, can obviously improve and use CO merely
2The attractant effect of mosquito luring lamp.Poisoning will greatly reduce the mosquito population quantity in the adult mosquito union if lure at the initial stage that mosquito is taken place, and reduce dwell mosquito harassing and wrecking and thorn of family and sting probability, will become the more efficiently method of prevention and control mosquito matchmaker infectious disease.
The present invention will be further described by the following examples, and embodiment comprises but do not limit protection scope of the present invention.
Embodiment
Embodiment 1
In the laboratory, mixed as attractant in 1: 1 by volume with sad 10 μ g/mL, indoles 0.1 μ L/mL.Measure the attracting action of attractant with T-shape olfactometer (with reference to Geier et al., 1996 design has also been done some and revised) to the Culex pipiens pallens female adult worm.T-shape olfactometer base brachium 35cm, two each long 20cm of " T " arm, diameter 7cm.Basic arm is connected the mosquito release room; Article two, " T " arm end extends 2 sample rooms, and air communication is crossed " Y " pipe and linked to each other with the sample room.Before air-flow entered the sample room, air was earlier through activated carbon filtration and distilled water humidification, and the gas flow of regulating in the olfactometer is 900ml/min.26～28 ℃ of room temperatures, relative moisture 70～80%, the luminous intensity in the olfactometer is 100～150lux.Draw 30 female mosquitos with mosquito sucking tube and insert people mosquito release room, after mosquito adapts to 15 minutes, (5cm * 5cm) put into left and right sample room at random with the identical filter paper (in contrast) that is soaked with the 0.5mL solvent, record enters the mosquito number of handling in arm and the control arm behind the timing 20min for the filter paper that tries attractant solution will to be soaked with 0.5mL respectively.
Each sample repeats 6 times, tests 30 female mosquitos at every turn, and all transposing is handled and control site at every turn, may be to the influence of left arm or right arm trend to eliminate mosquito.
The result: attractant arm end can attract about 85% mosquito.The female mosquito of Culex pipiens pallens produces the approach behavior reaction to attractant, and attractant has tangible sucking action to the female mosquito of Culex pipiens pallens.
Embodiment 2:
In the laboratory, mix as attractant by 1: 1 volume ratio with sad 10 μ g/mL, indoles 0.1 μ L/mL.Measure the attracting action of compound with the T-shape olfactometer to the aedes albopictus female adult worm.Method of testing is the same.
The result: attractant arm end can attract about 88% mosquito.The female mosquito of aedes albopictus produces the approach behavior reaction to attractant, and attractant has tangible sucking action to the female mosquito of aedes albopictus.
Embodiment 3:
Open-air release test.This experiment is carried out according to around the people room, village in Kunming Xun Dian county I in August, 2009.Adopt Chinese Microbiology and Epidemic Disease Inst., Academy of Military-Medical Sciences (C and Beijing grand hat development in science and technology Co., Ltd to unite the mosquito trapper MT-1 (GB/T5836.2) of manufacturing, trapper apart from ground 1.5m about, respectively at interval more than 50 meters, trapper CO
2The air communication passing through fan is blown into mosquito in the bag.Sad, indoles are made into 10 μ g/mL and 1 μ L/mL concentration respectively with ethanol, and the vial of packing into after the mixing in 1: 1 by volume is suspended on by the mosquito luring lamp.Trapping from afternoon 5:00 to 10:00 in evening.Adopt attractant+CO
2Mosquito luring lamp and CO
2Mosquito luring lamp contrasts simultaneously, tested altogether 3 days, with 3 days lure the mosquito result average, result such as following table:
In the last table,, as the trapping base value seizure mosquito number and this base value of attractant compared with the trapping mosquito number of no attractant, the mosquito number that attractant is caught Duos 20.6% than this base value, proves that attractant has tangible enhancing attractant effect.
Embodiment 4:
Open-air release test.With attractant+CO
2Mosquito luring lamp and lactic acid+CO
2Mosquito luring lamp is compared, and all the other are identical with embodiment 3 conditions.Trapping since afternoon 4:30 simultaneously to evening 10:30 finish, tested altogether 3 days, with 3 days lure the mosquito result average.
Result: attractant+CO
2The comparison of mosquito number that mosquito luring lamp is caught shows that according to many 16.1% the attractant effect is better than lactic acid.
Claims (7)
1. the attractant of culex and yellow-fever mosquito is characterized in that this attractant is mixed by sad and indoles to form, and wherein, sad concentration is 10 μ g/mL, and indoles concentration is 0.1～1 μ g/mL, both 1: 1 by volume mixed preparing.
2. attractant according to claim 1 is characterized in that as indoor attractant, and its sad concentration is that 10 μ g/mL, indoles concentration are 0.1 μ g/mL.
3. attractant according to claim 1 is characterized in that the attractant as open-air usefulness, and its sad concentration is that 10 μ g/mL, indoles concentration are 1 μ g/mL.
4. according to claim 1,2 or 3 described attractants, it is characterized in that this attractant with ethanol as sad and solvent indoles.
5. the method for application such as the described attractant of claim 1～4 is characterized in that attractant is placed indoor or open-air dispensing naturally.
6. attractant application method according to claim 5, when it is characterized in that open-air release, attractant is contained in the glass long-neck volumetric flask or is contained in the interior dispensing of glass flask of other tool plug.
7. according to claim 5 or 6 described attractant application methodes, it is other or be suspended within the insect attractant device coverage scope that the bottle that it is characterized in that being equipped with attractant is suspended on the insect attractant device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101987313A CN101843245B (en) | 2010-06-12 | 2010-06-12 | Culex and aedes attractant and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101987313A CN101843245B (en) | 2010-06-12 | 2010-06-12 | Culex and aedes attractant and application method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101843245A true CN101843245A (en) | 2010-09-29 |
| CN101843245B CN101843245B (en) | 2013-11-20 |
Family
ID=42768225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101987313A Expired - Fee Related CN101843245B (en) | 2010-06-12 | 2010-06-12 | Culex and aedes attractant and application method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101843245B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125037A (en) * | 2011-01-12 | 2011-07-20 | 马晓光 | Mosquito trapping liquid |
| CN107114412A (en) * | 2017-05-22 | 2017-09-01 | 和县乌江镇雅轩家庭农场 | A kind of flies lure agent |
| WO2019028585A1 (en) * | 2017-08-07 | 2019-02-14 | 广州威佰昆生物科技有限公司 | Aedes albopictus and production method therefor |
| CN113367141A (en) * | 2021-05-14 | 2021-09-10 | 深圳市蓝禾技术有限公司 | Mosquito attractant and application thereof |
-
2010
- 2010-06-12 CN CN2010101987313A patent/CN101843245B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 余静: "淡色库蚊嗜血习性的嗅觉生理研究", 《军事医学科学院博士学位论文》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125037A (en) * | 2011-01-12 | 2011-07-20 | 马晓光 | Mosquito trapping liquid |
| CN107114412A (en) * | 2017-05-22 | 2017-09-01 | 和县乌江镇雅轩家庭农场 | A kind of flies lure agent |
| WO2019028585A1 (en) * | 2017-08-07 | 2019-02-14 | 广州威佰昆生物科技有限公司 | Aedes albopictus and production method therefor |
| CN113367141A (en) * | 2021-05-14 | 2021-09-10 | 深圳市蓝禾技术有限公司 | Mosquito attractant and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101843245B (en) | 2013-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ponnusamy et al. | Oviposition responses of the mosquitoes Aedes aegypti and Aedes albopictus to experimental plant infusions in laboratory bioassays | |
| CN1846496B (en) | Method and compositions for attracting mosquitoes employing (R)-(-) isomers of 1-alken-3-ols | |
| CN101843245B (en) | Culex and aedes attractant and application method | |
| US20160270393A1 (en) | Insect repellent compositions and methods of use | |
| CN106973896B (en) | Rice leaf roller adult attractant and application thereof | |
| CN104094928A (en) | Mango deporaus marginatus pascoe attractant and preparation method thereof | |
| CN106942177A (en) | A kind of insect traps device, preparation method and application | |
| CN102125037A (en) | Mosquito trapping liquid | |
| WO2019186123A1 (en) | Mosquito attractants | |
| CN115769818A (en) | Application of (cis) -3-hexenol acetate in inducing spodoptera frugiperda to lay eggs and serving as spodoptera frugiperda attractant | |
| CN106305773B (en) | A kind of mosquito trap agent | |
| CN109042653A (en) | It is a kind of prevent and treat oriental armyworm sex attractant and its application | |
| JP4127721B2 (en) | Aedes aegypti or mosquito coils for controlling Aedes aegypti | |
| CN110859194B (en) | Compound mosquito-repelling essential oil, application and mosquito repellent | |
| CN108432756B (en) | Sex pheromone for preventing and controlling phaea cinnabarina and preparation method of lure core of sex pheromone | |
| WO2010101462A2 (en) | Behaviour-modifying odorant mixture for malaria mosquitoes | |
| CN101669469B (en) | Sex attractant for isoceras sibirica | |
| CN104126580B (en) | A kind of Mikania micrantha neck fleahopper imago attractant | |
| CN108260603A (en) | A kind of pest of stored grain controlling agent of the repellant containing Java brucea | |
| CN115336599A (en) | Spodoptera frugiperda biological food attractant and application thereof | |
| CN103875660B (en) | A kind of eucalyptus shoot gall Ji chalcid fly attractant | |
| Hartfield et al. | Pheromone traps for the dissemination of an entomopathogen by the damson-hop aphid Phorodon humuli | |
| CN114304186A (en) | Mandarin orange psylla trapping agent and application thereof | |
| WO2010130990A2 (en) | Use of a carboxylic acid or an aldehyde | |
| Yan et al. | The effect of transfluthrin-treated jute and cotton emanator vests on human landing and fecundity of Anopheles minimus in Thailand |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131120 Termination date: 20140612 |
|
| EXPY | Termination of patent right or utility model |