CN108432756B - Sex pheromone for preventing and controlling phaea cinnabarina and preparation method of lure core of sex pheromone - Google Patents
Sex pheromone for preventing and controlling phaea cinnabarina and preparation method of lure core of sex pheromone Download PDFInfo
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- CN108432756B CN108432756B CN201810331800.XA CN201810331800A CN108432756B CN 108432756 B CN108432756 B CN 108432756B CN 201810331800 A CN201810331800 A CN 201810331800A CN 108432756 B CN108432756 B CN 108432756B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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Abstract
A sex pheromone for preventing and controlling the phaea cinnabarina and a preparation method of a lure thereof, the phaea cinnabarina sex pheromone consists of cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecatrienol according to the mass ratio of 1:1, and the preparation method of the lure comprises the following steps: under the condition of room temperature, firstly, mixing 10 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 980 microliters of n-hexane solution to form a mixed solution with the mass ratio of two sex pheromones being 1: 1; then, dripping the mixed liquid containing the sex pheromone of the phaea virgata onto the cotton sliver lure core, and preparing the lure core containing the phaea virgata lure agent after n-hexane in the mixed liquid containing the sex pheromone of the phaea virgata volatilizes; finally, the prepared lure is put into an aluminum foil for sealing and is refrigerated at the temperature of minus 20 ℃. The sex pheromone for preventing and controlling the phaea virens has strong specificity, has obvious trapping effect on the phaea virens in the wild and has stable effect.
Description
Technical Field
The invention belongs to the technical field of insect sex pheromone preparation, and particularly relates to a sex pheromone for preventing and controlling phaea cinnabarina and a preparation method of a lure core thereof.
Background
The Epinephelus cinnabarinus Walker (Lepidoptera: Zygaeneidae) is an important leaf-eating pest on banyans. In recent years, intermittent outbreak of harm occurs in places such as Guangxi, Guangdong, Fujian and Hainan, and countries such as India, Vietnam and Thailand, and human living environment, urban landscape, image and ecological benefit are seriously affected. At present, chemical control is mainly used for controlling the phaea cinnabarina, but hosts which are harmful to the phaea cinnabarina are often planted at two sides of a road with large pedestrian flow or in parks, the body health of human beings is seriously threatened by using a large amount of chemical agents, and the problems of drug resistance of the phaea cinnabarina, mistaken killing of natural enemies, environmental pollution and the like are easily caused. Biological control is an alternative environment-friendly control technology.
The main component of the insect sex attractant is insect sex pheromone from insect bodies, and the insect sex pheromone does not cause harm to human health and environment. Thus, biological control methods are increasingly being applied to field monitoring and pest trapping. According to the principle that female insects release sex pheromone to lure male insects, the sex pheromone components and proportion of the phaea virens are determined, and the phaea virens sex pheromone can be accurately applied to insect condition prediction, mating interference and mass trapping, so that the ideal effect of green prevention and control of the phaea virens is achieved.
Disclosure of Invention
The invention aims to provide a sex pheromone for preventing and controlling the epirubia cinnabarina and a preparation method of a lure thereof, so as to improve the effect of controlling the epirubia cinnabarina population.
In order to achieve the aim, the sex pheromone for preventing and controlling the phaea cinnabarina consists of cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecenal.
The sex pheromone of the phaea cinnabarina consists of cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecatrienol according to the mass ratio of 1: 1.
Cis-9-Hexadecenal and (Z, Z, Z) -9,12, 15-octadecatrienol are all referred to as Cis-9-Hexadecenal (Cis-9-Hexadecenal) and (Z, Z, Z) -9,12, 15-octadecatrienol ((Z, Z, Z) -9,12, 15-octadecatrienal).
A preparation method of a sex pheromone lure of phaea cinnabarina comprises the following steps: under the condition of room temperature, firstly, mixing 10 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 980 microliters of n-hexane solution to form a mixed solution with the mass ratio of two sex pheromones being 1: 1; secondly, dripping the mixed liquid containing the sex pheromone of the phaea virgata onto the cotton sliver lure core, and preparing the lure core containing the phaea virgata lure agent after n-hexane in the mixed liquid containing the sex pheromone of the phaea virgata volatilizes; finally, the prepared lure is put into an aluminum foil for sealing and is refrigerated at the temperature of minus 20 ℃.
When the sex pheromone of the phaea virens is used for trapping the phaea virens, the trap containing the sex pheromone lure core of the phaea virens is hung in an area where the phaea virens occurs or is likely to occur, and the phaea virens is used for monitoring the pest situation of the phaea virens, trapping a large amount of phaea virens and disturbing mating. The trap is a boat-shaped trap.
The invention has the outstanding advantages that:
1. the sex pheromone component of the phaea cinnabarina is put forward for the first time, the manufacturing method of the sex pheromone lure core is simple, and the raw materials are easy to obtain; the compound has strong trapping capability, long lasting period and strong specificity, and can not cause the phaea cinnabarina to generate resistance; is harmless to human and livestock and environment-friendly; strong stress resistance and convenient use.
2. The sex pheromone for preventing and controlling the phaea virens has an obvious trapping effect on the phaea virens in the field, and the effect is stable. The total moth attracting amount of the Plutella xylostella sex pheromone attracting core is up to 349 days, the average moth attracting amount of the first day, the second day and the third day is obviously more than that of each control group, and no obvious difference exists among the day-average moth attracting amounts of all traps.
3. Can be accurately applied to insect condition prediction, mating interference and mass trapping, and has important significance for preventing and controlling the population of the phaea cinnabarina.
Detailed Description
The technical solutions of the present invention are described in detail by the following examples, but it should be understood that the scope of the present invention is not limited by the specific embodiments.
Example 1
Extraction of sex pheromone of phaea cinnabarina
Sex pheromone of unmatched female moths of different ages in days and in light periods and different time is extracted by a headspace adsorption method and a gonad extraction method, and sex information of the moths of the red moths is obtained by detecting the moths of the red moths by a gas chromatography-mass spectrometer and testing the moths by an insect antennal potential measuring system, wherein the sex information of the moths of the red moths consists of cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecatrienal.
Example 2
Preparation of sex pheromone lure of phaea cinnabarina
Under the condition of room temperature, firstly, mixing 10 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 980 microliters of n-hexane solution to form a mixed solution with the mass ratio of two sex pheromones being 1: 1; secondly, dripping the mixed liquid containing the sex pheromone of the phaea virgata onto the cotton sliver lure core, and preparing the lure core containing the phaea virgata lure agent after n-hexane in the mixed liquid containing the sex pheromone of the phaea virgata volatilizes; finally, the prepared lure is put into an aluminum foil for sealing and is refrigerated at the temperature of minus 20 ℃.
Example 3
Wild trapping effect of sex pheromone of phaea cinnabarina
The following 8 kinds of lure cores were set:
adding 10 microliters of cis-9-hexadecenal into 990 microliters of n-hexane solution, dropwise adding the mixed solution onto the cotton sliver lure core, and obtaining the lure core containing the luring agent for the rubisco caterpillar after the n-hexane is volatilized;
b, adding 10 microliters of (Z, Z, Z) -9,12, 15-octadecenal into 990 microliters of n-hexane solution, dropwise adding the mixed solution onto the cotton sliver lure, and obtaining the lure containing the Plutella xylostella lure after n-hexane is volatilized;
c, adding 10 microliters of trans-9-hexadecenal into 990 microliters of n-hexane solution, dropwise adding the mixed solution onto the cotton sliver lure core, and obtaining the lure core containing the Plutella xylostella lure agent after the n-hexane is volatilized;
and D, adding 10 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 980 microliters of n-hexane solution, dripping the mixed solution onto the cotton sliver lure core, and obtaining the lure core of the sex pheromone of the phaedomyza cinnabarina with the mass ratio of 1:1 after the n-hexane is volatilized. In order to ensure the release effect, 50-1000 mug of sex pheromone is put into each 1mL of cotton sliver lure core;
and E, adding 10 microliters of cis-9-hexadecenal and 20 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 970 microliters of n-hexane solution, dripping the mixed solution onto the cotton sliver lure, and obtaining the lure of the sex pheromone of the phaedomonas cinnabarina with the mass ratio of 1:2 after the n-hexane is volatilized. In order to ensure the release effect, 50-1000 mug of sex pheromone is put into each 1mL of cotton sliver lure core;
f, adding 20 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 970 microliters of n-hexane solution, dripping the mixed solution onto the cotton sliver lure, and obtaining the lure of the sex pheromone of the phaedomonas cinnabarina with the mass ratio of 2:1 after the n-hexane is volatilized. In order to ensure the release effect, 50-1000 mug of sex pheromone is put into each 1mL of cotton sliver lure core;
g, a lure containing only n-hexane;
h, only containing 3-5 lures of 1-day-old virgina of the epirubia virescens.
5 ship-shaped traps are configured for each treatment, the trap cores are placed in the ship-shaped traps, the traps are uniformly and randomly placed in an area where the Plutella xylostella is severe, the average distance between the traps is 20-30m, and the height from the ground is 5-6 m. After the incubation, the number of male Plutella xylostella induced and caught was investigated every day, the female Plutella xylostella in the lure core H was replaced with the live female Plutella xylostella which emerged the day, and the positions of each treatment were randomly replaced, and the experiment was continued for 3 days.
The total amount of 3-day moth lures containing the sex pheromone D lures of the Plutella xylostella is up to 349 heads, the average moth lures on the first day, the second day and the third day are all obviously more than those of each control group, and the daily average moth lures of each trap have no obvious difference. The sex pheromone has strong specificity, has obvious trapping effect on the phaea virens in the wild and has stable effect. The trapping effect is shown in table 1.
TABLE 1 Effect of the sex pheromone of the Choroid moth in the wild trapping (Unit: head)
In Table 1, A represents a lure containing only cis-9-hexadecenal; b represents a lure containing only (Z, Z, Z) -9,12, 15-octadecenal; c represents a lure containing only trans-9-hexadecenal; d represents a lure with the mass ratio of cis-9-hexadecenal to (Z, Z, Z) -9,12, 15-octadecatrienol being 1: 1; e represents a lure with the mass ratio of cis-9-hexadecenal to (Z, Z, Z) -9,12, 15-octadecatrienol being 1: 2; f represents a lure core with the mass ratio of cis-9-hexadecenal to (Z, Z, Z) -9,12, 15-octadecatrienol being 2: 1; g represents a lure containing only n-hexane; h represents the lure of 1-day-old virginator containing only 3-5 heads of the rubisco virescens. The same lower case letters in the same column indicate insignificant difference (significance level P >0.05) as determined by the chey test, and the different upper case letters in the same row indicate significant difference (significance level P <0.05) as determined by the chey test.
As can be seen from the test results of table 1: a lure core only containing cis-9-hexadecenal, B lure core only containing (Z, Z, Z) -9,12, 15-octadecenal, C lure core only containing trans-9-hexadecenal, E lure core only containing cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecenal with the mass ratio of 1:2, F lure core containing cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecenal with the mass ratio of 2:1, G lure core only containing normal hexane, H lure core only containing 3-5 heads of Ardisia cinnabarina 1-day-old living female insects, and D lure core only containing cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecenal with the mass ratio of 1:1 are compared, the experimental results show that: in a field trapping experiment, a trapping core of sex pheromone of the phaea haematocephala is A, B, C, D, E, F, G, H is used for trapping, only D represents a trapping core with the mass ratio of cis-9-hexadecenal to (Z, Z, Z) -9,12,15-octadecatrienal of 1:1, the trapping effect is the best, the total amount of 3 days of moth trapping reaches 349 heads, and the effect is stable. It was demonstrated that sex pheromones consisting of cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecatrienol in a mass ratio of 1:1 have a very high specificity and cannot be replaced by other similar structures and different mass ratios.
The above disclosure is only one specific embodiment of the present invention. However, the present invention is not limited thereto, and any variations that can be conceived by those skilled in the art should fall within the scope of the present invention.
Claims (5)
1. The sex pheromone for preventing and controlling the phaea cinnabarina is characterized by consisting of cis-9-hexadecenal and (Z, Z, Z) -9,12, 15-octadecatrienol, and consists of the cis-9-hexadecenal and the (Z, Z, Z) -9,12, 15-octadecatrienol in a mass ratio of 1:1,
the preparation method of the sex pheromone lure of the phaea virens comprises the following steps:
under the condition of room temperature, firstly, mixing 10 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z) -9,12, 15-octadecatrienol into 980 microliters of n-hexane solution to form a mixed solution with the mass ratio of two sex pheromones being 1: 1; then, dripping the mixed liquid containing the sex pheromone of the phaea virgata onto the cotton sliver lure core, and preparing the lure core containing the phaea virgata lure agent after n-hexane in the mixed liquid containing the sex pheromone of the phaea virgata volatilizes; finally, the prepared lure is put into an aluminum foil for sealing and is refrigerated at the temperature of minus 20 ℃.
2. The application of the sex pheromone prepared by the method in the claim 1 in the biological control of the phaea cinnabarina.
3. The use of the sex pheromone for controlling phaea cinnabarina prepared in claim 1 for biocontrol of phaea cinnabarina, characterized in that, when the phaea cinnabarina is trapped, a trap containing a phaea cinnabarina sex pheromone lure is hung in an area where phaea cinnabarina occurs or is likely to occur, for monitoring the pest situation of phaea cinnabarina, trapping a large amount and interfering with mating.
4. The use of the sex pheromone for controlling phaea virens prepared in claim 3 for biocontrol of phaea virens, wherein said trap is a boat-type trap.
5. The use of sex pheromones for the control of phaea virescens according to claim 4 for the biological control of phaea virescens, wherein the boat-type traps are uniformly and randomly placed in the areas where phaea virescens occur more severely, and the average spacing between the traps is 20-30m, and the ground height is 5-6 m.
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| CN114145295B (en) * | 2021-09-16 | 2022-06-03 | 山东农业大学 | Sex pheromone of agrothiao furcellarae and application thereof |
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| CN101617652A (en) * | 2008-07-02 | 2010-01-06 | 宁波纽康生物技术有限公司 | Striped rice borer sex pheromone composition and inducement core thereof |
| CN101849538A (en) * | 2009-05-22 | 2010-10-06 | 深圳中昆生物防治服务股份有限公司 | Attractive core for preventing and controlling homoeosoma nebulella hubner |
| CN104186469A (en) * | 2014-09-12 | 2014-12-10 | 陕西理工学院 | Sex attractant and attraction core for attracting chilo suppressalis |
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