CN115067331A - Chilo suppressalis attractant composition and application thereof - Google Patents
Chilo suppressalis attractant composition and application thereof Download PDFInfo
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- CN115067331A CN115067331A CN202210656069.4A CN202210656069A CN115067331A CN 115067331 A CN115067331 A CN 115067331A CN 202210656069 A CN202210656069 A CN 202210656069A CN 115067331 A CN115067331 A CN 115067331A
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- hexadecenal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of insect attractants, and particularly relates to a Chilo suppressalis attractant composition and application thereof. A Chilo suppressalis attractant composition comprises the following components: the mass ratio of the sex pheromone to the plant volatile matter is 20-70: 1-20; the sex pheromone consists of cis-11-hexadecenal, cis-13-octadecenal and cis-9-hexadecenal, and the plant volatile matter consists of one or two of beta-caryophyllene, trans-2-hexenal or methyl jasmonate. The bait core of the invention is put into a trap, can be used for monitoring the field occurrence dynamics of chilo suppressalis, predicting the hazard degree of chilo suppressalis, guiding other prevention and treatment measures to be carried out timely, and has remarkable ecological benefit and social benefit.
Description
Technical Field
The invention belongs to the technical field of insect attractants, and particularly relates to a Chilo suppressalis attractant composition and application thereof.
Background
Chilo suppressalis is one of the most important pests on rice in China, in recent years, due to the reasons of tillage change, variety replacement, global climate warming and the like, the living environment of the Chilo suppressalis is greatly improved, the population quantity of the Chilo suppressalis is in a rising trend, and the pest is aggravated. At present, the method for controlling chilo suppressalis mainly relies on chemical pesticides, but public attention on food safety, environmental pollution and the like is attracted, and because larvae bore rice shortly after hatching, biological control becomes difficult.
In 1975-. Tatsuki et al discovered the 3 rd effective component cis-9-hexadecenal in 1983, and proved by a large number of field tests, the active ingredient has a strong attraction effect on the male moths of the chilo suppressalis, and the behavioral reactions of the male moths of the chilo suppressalis such as flapping, landing and the like in a short distance are obviously improved. The behavior test is carried out on the three chilo suppressalis sex pheromone components in a large proportion, and the optimal proportion of cis-11-hexadecenal, cis-13-octadecenal and cis-9-hexadecenal in the mass ratio of 48: 6: 5 is finally determined. It is a common formulation at home and abroad. However, a large number of field tests and applications find that the attracting effect of the core made by the proportion is not ideal, and particularly pests are difficult to be trapped when the population density in the field is low.
Plant volatile matters are common odorous substances and can play a role in attracting or avoiding pests. The synergistic effect of plant volatile matter and insect pheromone is mainly shown in that the generation and release of pest pheromone are stimulated, the synergistic effect of plant volatile matter on insect pheromone is achieved, and phytophagous insects search food and spawning sites according to volatile matters released by host plants and the like. There are two ways of releasing plant volatile matter, one is the volatile matter which is released naturally and plays an important role in host selection for phytophagous insects, it is composed of a plurality of micro volatile secondary matters emitted from the surface of the overground part of the plant, and is some organic chemical substances with the molecular weight of 100-200, including the mixture of alcohol, aldehyde, ketone, vinegar and terpenoid; the other is insect pest induced release, and the main components of the pesticide are 5 major groups, namely green leaf odor, terpenoid, nitrogen-containing compound, sulfur-containing compound and other chemical substances, and the volatile substances can be used as chemical substances for defending against feeding of phytophagous insects on one hand and can also be used as signal substances for searching and parasitizing the phytophagous insects and natural enemies thereof on the other hand.
At present, the properties and the action mechanism of plant volatile secondary substances are explored; the synergistic effect between plant volatile matters and the sex pheromone is explored, and a basis can be provided for a new strategy for pest control.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the following technical scheme:
a Chilo suppressalis attractant composition comprises the following components: the mass ratio of the sex pheromone to the plant volatile matter is 20-70: 1-20; the sex pheromone consists of cis-11-hexadecenal, cis-13-octadecenal and cis-9-hexadecenal, and the plant volatile matter consists of one or two of beta-caryophyllene, trans-2-hexenal or methyl jasmonate.
Preferably, the mass ratio of the cis 11-hexadecenal, the cis 13-octadecenal and the cis 9-hexadecenal is 48:1-10: 1-10.
Preferably, the plant volatile matter consists of beta-caryophyllene, trans-2-hexenal and methyl jasmonate; the mass ratio of the beta-caryophyllene, the trans-2-hexenal and the methyl jasmonate is 1-5:1-5: 1-5.
The invention also provides a Chilo suppressalis attractant core, the attractant composition is prepared into the attractant core, the effective component contained in a single attractant core is 10-500 mu g, and the solvent adopts heavy-distilled n-hexane.
Preferably, an antioxidant and an ultraviolet light resistant stabilizer are further added into the lure.
The attractant composition and the attractant core provided by the invention can be used for preventing and controlling rice-stem borers.
The attractant composition and the attractant core provided by the invention can be used for monitoring Chilo suppressalis populations.
By adopting the technical scheme, the invention has the following beneficial effects:
(1) according to the invention, a large number of field tests prove that the trapping and killing effect of the striped rice borer sex pheromone can be greatly improved by adding the plant volatile matter, the trapping amount is large, the trapping effect is stable, and the problems of poor prevention and control effect and high cost of striped rice borer sex pheromone at present are solved.
(2) When the population density in the field is low, the attraction effect of the sex pheromone which is singly adopted to the chilo suppressalis is poor. After the plant volatile substance trans-2-hexenal, methyl jasmonate and beta-caryophyllene are used in combination with the sex pheromone, the attraction effect is obviously improved. There was a significant difference between the attracting effect with the other treatments.
(3) The attractant is simple and safe to use, has low cost, can be applied to prediction, prediction and prevention and control of chilo suppressalis, and has good social, economic and ecological benefits.
Detailed Description
The present invention will be further explained with reference to specific embodiments.
First, attractant combination embodiment
Example 1
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal, 1 part of trans-2-hexenal, 1 part of methyl jasmonate and 1 part of beta-caryophyllene.
Example 2
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 10 parts of cis-13-octadecenal, 10 parts of cis-9-hexadecenal, 1 part of trans-2-hexenal, 2 parts of methyl jasmonate and 1 part of beta-caryophyllene.
Example 3
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis 11-hexadecenal, 3 parts of cis 13-octadecenal, 5 parts of cis 9-hexadecenal, 2 parts of trans-2-hexenal, 1 part of methyl jasmonate and 1 part of beta-caryophyllene.
Example 4
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 5 parts of cis-13-octadecenal, 2 parts of cis-9-hexadecenal, 3 parts of trans-2-hexenal, 1 part of methyl jasmonate and 2 parts of beta-caryophyllene.
Example 5
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 4 parts of cis-13-octadecenal, 1 part of cis-9-hexadecenal, 2 parts of trans-2-hexenal, 2 parts of methyl jasmonate and 1 part of beta-caryophyllene.
Example 6
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 1 part of cis-9-hexadecenal, 2 parts of trans-2-hexenal, 1 part of methyl jasmonate and 3 parts of beta-caryophyllene.
Example 7
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 1 part of cis-13-octadecenal, 7 parts of cis-9-hexadecenal, 1 part of trans-2-hexenal, 2 parts of methyl jasmonate and 2 parts of beta-caryophyllene.
Example 8
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 4 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal, 2 parts of trans-2-hexenal, 3 parts of methyl jasmonate and 2 parts of beta-caryophyllene.
Example 9
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 3 parts of cis-13-octadecenal, 2 parts of cis-9-hexadecenal, 3 parts of trans-2-hexenal, 3 parts of methyl jasmonate and 3 parts of beta-caryophyllene.
Example 10
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 8 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal, 2 parts of trans-2-hexenal, 1 part of methyl jasmonate and 2 parts of beta-caryophyllene.
Comparative example 1
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal and 3 parts of trans-2-hexenal.
Comparative example 2
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal and 3 parts of methyl jasmonate.
Comparative example 3
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal and 3 parts of beta-caryophyllene.
Comparative example 4
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal, 1 part of trans-2-hexenal and 1 part of methyl jasmonate.
Comparative example 5
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal, 5 parts of cis-9-hexadecenal, 1 part of methyl jasmonate and 1 part of beta-caryophyllene.
Comparative example 6
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis 11-hexadecenal, 6 parts of cis 13-octadecenal, 5 parts of cis 9-hexadecenal, 1 part of trans-2-hexenal and 1 part of beta-caryophyllene.
Comparative example 7
A Chilo suppressalis attractant composition comprises the following components in parts by weight:
48 parts of cis-11-hexadecenal, 6 parts of cis-13-octadecenal and 5 parts of cis-9-hexadecenal.
Secondly, manufacturing the lure core
Organic silica gel lure cores are adopted, 10-500 mu g of evenly mixed attractant composition is added into each lure core, and the solvent is redistilled n-hexane.
Third, field test
1 general description of the test
The test is carried out in a rice planting core area of the Zorochamus townhua in the east city of Hainan province, rice is planted in a piece-by-piece mode in the area, the rice management is good, and soil is muddy soil.
2 protocol of the test
2.1 test materials
Cis 11-hexadecenal, cis 9-hexadecenal, cis 13-octadecenal, trans-2-hexenal, methyl jasmonate, beta-caryophyllene; and a lure prepared from the above substances.
2.2 design of the experiment
Selecting a paddy field with flat terrain and consistent variety, cultivation, pesticide application, fertilizer application and the like as a test field, and selecting a field with a degree of emergence more than the medium degree by combining the emergence condition of the local rice stem borers in the past years.
The luring cores are placed into the traps, the traps subjected to different treatments are randomly arranged in a checkerboard shape, and the traps are uniformly inserted at the edge of the paddy field at the emergence initial stage of the first generation chilo suppressalis adults, so that the insect inlet of the traps is flush with the top of the paddy rice.
2.3 test time
Test time: year 2020, 3, 8;
the trap adjustment and replacement lure time: year 2020, 3, 15;
test result time: year 2020, 3, 23.
2.3 test investigation
The test was treated with drug and control, repeated 3 times, and each cell was randomly arranged. The investigation was started the next day after the installation of each treatment, and the daily investigation recorded the number of chilo suppressalis trapped in each treatment (the chilo suppressalis residues in the traps were cleared at each investigation) until the end of the experiment.
2.4 test results
The phytophagous insects search for host plants mainly through olfaction in the host searching stage, and a plant odor chemical fingerprint diagram consisting of different concentrations of volatile matters released by the plants in the environment is the key for the insects to identify the hosts. Meanwhile, the composition of plant volatile matters can obviously influence the attraction effect of the sex pheromone.
TABLE 1 measurement of synergistic Effect of plant volatiles and pheromones
As can be seen from Table 1, when the population density in the field is low, the attraction effect of sex pheromone alone on chilo suppressalis in comparative example 7 is low, and is only 9.33 heads/cell. After the sex pheromone is respectively combined with trans-2-hexenal or methyl jasmonate, the attraction quantity is improved to a certain extent, but the attraction effect is not obviously different from that of the comparative example 7. After the sex pheromone and the beta-caryophyllene are used in combination, the attraction effect is obviously improved and reaches 18.33 heads/cell. In the example 1, after the plant volatile matter is combined with trans-2-hexenal, methyl jasmonate and beta-caryophyllene and the sex pheromone, the attraction effect reaches 44.67 heads/cell. There was a significant difference between the attracting effect with the other treatments.
TABLE 2 trap trapping amount per treatment
As can be seen from table 2, compared to comparative example 7, the attractant composition of the present invention has an excellent attracting effect on rice chilo suppressalis when the population density in the field is low, and particularly the attractant compositions of examples 4, 6 and 10 have a better attracting effect on rice chilo suppressalis, which are 95.33 heads/cell, 75.33 heads/cell and 69.00 heads/cell, respectively.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same.
Claims (6)
1. A Chilo suppressalis attractant composition, which is characterized in that: comprises the following components: the mass ratio of the sex pheromone to the plant volatile matter is 20-70: 1-20; the sex pheromone consists of cis-11-hexadecenal, cis-13-octadecenal and cis-9-hexadecenal, and the plant volatile matter consists of one or two of beta-caryophyllene, trans-2-hexenal or methyl jasmonate.
2. The Chilo suppressalis attractant composition of claim 1, wherein: the mass ratio of the cis 11-hexadecenal, the cis 13-octadecenal and the cis 9-hexadecenal is 48:1-10: 1-10.
3. The Chilo suppressalis attractant composition of claim 1, wherein: the plant volatile matter consists of beta-caryophyllene, trans-2-hexenal and methyl jasmonate; the mass ratio of the beta-caryophyllene, the trans-2-hexenal and the methyl jasmonate is 1-5:1-5: 1-5.
4. A Chilo suppressalis attractant core is characterized in that the attractant composition is prepared into an attractant core according to any one of claims 1-3, the effective component contained in a single attractant core is 10-500 mu g, and the solvent is heavy distilled n-hexane.
5. Use of the attractant composition according to any one of claims 1 to 3 or the lure according to claim 4 for the control of rice-stem borer.
6. Use of the attractant composition according to any one of claims 1-3 or the lure according to claim 4 for striped rice borer population monitoring.
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