CN117571437A - Quantitative sample cup-in extraction device - Google Patents
Quantitative sample cup-in extraction device Download PDFInfo
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- CN117571437A CN117571437A CN202311553193.9A CN202311553193A CN117571437A CN 117571437 A CN117571437 A CN 117571437A CN 202311553193 A CN202311553193 A CN 202311553193A CN 117571437 A CN117571437 A CN 117571437A
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- 238000000605 extraction Methods 0.000 title claims abstract description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 131
- 238000012360 testing method Methods 0.000 claims abstract description 80
- 238000001914 filtration Methods 0.000 claims abstract description 41
- 238000004146 energy storage Methods 0.000 claims abstract description 31
- 239000002994 raw material Substances 0.000 claims abstract description 22
- 239000003085 diluting agent Substances 0.000 claims abstract description 20
- 238000010790 dilution Methods 0.000 claims abstract description 19
- 239000012895 dilution Substances 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims description 62
- 239000007788 liquid Substances 0.000 claims description 43
- 238000010438 heat treatment Methods 0.000 claims description 34
- 239000002699 waste material Substances 0.000 claims description 19
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 12
- 229910052782 aluminium Inorganic materials 0.000 claims description 12
- 239000000741 silica gel Substances 0.000 claims description 12
- 229910002027 silica gel Inorganic materials 0.000 claims description 12
- 238000007865 diluting Methods 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 6
- 239000004411 aluminium Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 abstract description 8
- 210000004369 blood Anatomy 0.000 description 15
- 239000008280 blood Substances 0.000 description 15
- 230000006835 compression Effects 0.000 description 8
- 238000007906 compression Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000011534 incubation Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/02—Sealings between relatively-stationary surfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a quantitative sample cup-in-cup extraction device which comprises a storage bin, a filtering bin, a reaction bin and an energy storage bin which can be communicated in sequence. The storage bin is arranged for storing the diluent, and the test sample is directly added when the diluent is required to be diluted, so that the diluent and the test sample are uniformly mixed for dilution; the filtering bin is communicated with the storage bin, the diluted test sample directly flows into the filtering bin, unnecessary sample components are filtered out when the test sample passes through the filtering structure, and the required sample components are separated; the reaction bin is communicated with the filtering bin, the filtered test sample flows into the reaction bin, and the energy storage bin heats the reaction bin to enable the test sample to generate chemical reaction with the reaction raw materials in the reaction bin. The purification and reaction of the test sample are carried out through the cooperation of a plurality of structure bins, the back and forth movement among a plurality of large-scale devices is not needed, the occupation of the types of the devices and the space of the devices is reduced, and the contact and pollution of the test sample and the outside in the purification reaction process are avoided.
Description
Technical Field
The invention relates to the technical field of medical appliances, in particular to a quantitative sample cup-in-cup extraction device.
Background
The whole blood sample is mainly used for checking a red blood cell system, a white blood cell system and platelets, and the blood clinical laboratory can be used for primarily diagnosing anemia, viral infection, bacterial infection, blood coagulation function and the like by checking the diluted whole blood sample and observing the high and low indexes of the red blood cells, the white blood cells and the platelets. The simple whole blood sample detection can only meet the daily simple judgment, and cannot well make accurate judgment in the blood fields of clinical diagnosis, medical treatment, scientific research and the like, and the serum detection is a means which is widely and accurately used clinically, and can be used for diagnosing and monitoring various diseases. The existing serum preparation is relatively troublesome in laboratory and department inspection operation, and because the blood sample needs to be frequently moved to different instruments for different operations when the whole blood sample is separated and extracted, good tightness of the sample cannot be ensured in the moving process, the risk that the sample is polluted exists, and further, the deviation of the sample result can be caused. Meanwhile, as the sampling separation operation steps are more, a plurality of large-sized instruments are required to be equipped for separation and monitoring, the operation is complicated, the required space is large, and the space waste in the hospital area is easily caused.
Disclosure of Invention
Therefore, the invention aims to solve the technical problems of multiple types of whole blood sample extraction operation equipment and complicated operation in the prior art, thereby providing a quantitative sample cup-in-cup extraction device.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a quantitative sample cup-in-cup extraction device comprises a storage bin, a filtering bin, a reaction bin and an energy storage bin; the storage bin is adapted to store a diluent and a diluted test sample; the filter bin is suitable for being communicated with the storage bin, and a filter structure suitable for filtering a test sample is arranged on the filter bin; the reaction bin is suitable for being communicated with the filtering bin, and is suitable for storing reaction raw materials; the energy storage bin is suitable for providing heat for the reaction bin so as to enable the reaction raw materials to react with the test sample.
Further, a vibration structure, a dilution tank positioned at one side of the vibration structure and a sealing cover arranged at the top of the dilution tank in a covering manner are arranged in the storage bin, and the dilution tank is suitable for storing dilution liquid.
Further, a first liquid outlet hole is formed in the bottom of the dilution tank, and a first sealing film is arranged on the first liquid outlet hole; the filter cartridge is adapted to be connected to the bottom of the storage cartridge, and the top of the filter cartridge is provided with a first puncture needle corresponding to the first sealing membrane.
Further, the sealing cover comprises a push rod connected with the storage bin through a buckle, a silica gel sealing sleeve connected to the bottom end of the push rod, a compression cap connected to the top of the push rod and a safety cover connected between the silica gel sealing sleeve and the compression cap.
Further, a quantitative groove and the filtering structure positioned at the top of the quantitative groove are arranged in the filtering bin.
Further, a waste liquid tank positioned at the periphery of the quantitative tank is also arranged in the filtering bin, and a waste liquid hole positioned at the top of the quantitative tank is arranged between the waste liquid tank and the quantitative tank.
Further, a second liquid outlet hole is formed in the bottom of the quantitative groove, and a second sealing film is arranged on the second liquid outlet hole; the reaction bin is suitable for being connected to the bottom of the filtering bin, and a second puncture needle corresponding to the second sealing film is arranged at the top of the reaction bin.
Further, a reaction tank suitable for storing the reaction raw materials is arranged at the bottom of the reaction bin, the reaction tank is arranged corresponding to the quantitative tank in position, and the second puncture needle is arranged on the reaction tank.
Further, the energy storage bin is suitable for being connected to the bottom of the reaction bin; the top of the energy storage bin is provided with a heating structure, and a battery pack electrically connected with the heating structure is arranged in the energy storage bin.
Further, the heating structure is a heating aluminum block.
The technical scheme of the invention has the following advantages:
1. according to the quantitative sample cup-in-cup extraction device provided by the invention, the storage bin is arranged for storing the diluent, the test sample is directly added when dilution is required, and the diluent and the test sample are uniformly mixed in a shaking or vibrating mode to dilute the test sample; the filtering bin is communicated with the storage bin, the diluted test sample directly flows into the filtering bin, unnecessary sample components are filtered out when the test sample passes through the filtering structure, and the required sample components are separated; the reaction bin is communicated with the filtering bin, the filtered test sample flows into the reaction bin, and the energy storage bin heats the reaction bin to enable the test sample to generate chemical reaction with the reaction raw materials in the reaction bin. Through the cooperation in a plurality of structure warehouses, can carry out purification and the reaction of test sample, need not to reciprocate between a plurality of large-scale equipment, reduce the space occupation of equipment type and equipment, avoid the test sample to take place to contact with the external world in the purification reaction process, avoided the sample to be polluted, whole device is light and handy portable, operation convenient to use.
2. The quantitative sample cup-in-cup extracting device provided by the invention is characterized in that a vibrating structure, a diluting tank positioned on one side of the vibrating structure and a sealing cover arranged at the top of the diluting tank are arranged in a storage bin, and the diluting tank is suitable for placing diluent. The device can store the diluent through the diluent groove, and the test sample can be added by opening the sealing cover when the test sample needs to be diluted; the vibration structure can be used for uniformly mixing and diluting the diluent and the test sample in the diluting tank, so that the manual shaking is not needed, and the manpower can be effectively reduced when the number of the test samples to be processed is too large.
3. According to the quantitative sample cup-in-cup extraction device provided by the invention, the bottom of the dilution tank is provided with the first liquid outlet hole, and the first liquid outlet hole is provided with the first sealing film; the filter house is suitable for being connected in the storage bin bottom, and the filter house top is provided with the first puncture needle corresponding to first sealing membrane position. The storage bin is placed on the filter bin after the test sample is uniformly mixed and diluted by arranging the first sealing film and the first puncture needle, and the diluted test sample can flow into the filter bin by puncturing the first sealing film through the first puncture needle; the device can not contact with the outside in the process of transferring the test sample, and ensures the tightness of the test sample and the accuracy of the subsequent detection result.
4. The invention provides a quantitative sample cup-shaped extraction device, which comprises a push rod connected with a storage bin through a buckle, a silica gel sealing sleeve connected to the bottom end of the push rod, a compression cap connected to the top of the push rod and a safety cap connected between the silica gel sealing sleeve and the compression cap. The arrangement can ensure the tightness of the sealing cover on the storage bin through the silica gel sealing sleeve; through setting up the insurance lid between compressing tightly cap and silica gel seal cover, can avoid sealed lid to break away from the storage storehouse and influence its sealing performance when touched by mistake.
5. The quantitative sample cup-in extraction device provided by the invention is characterized in that a quantitative groove and a filtering structure positioned at the top of the quantitative groove are arranged in a filtering bin. The arrangement can be used for separating and filtering the test sample before entering the quantitative tank, so that the test sample flowing into the quantitative tank is ensured to be filtered and purified.
6. The quantitative sample cup-in-cup extraction device provided by the invention is characterized in that the filtering bin is also internally provided with a waste liquid tank positioned at the periphery of the quantitative tank, and a waste liquid hole positioned at the top of the quantitative tank is arranged between the waste liquid tank and the quantitative tank. By this arrangement, the liquid can be discharged into the waste liquid tank through the waste liquid hole when the liquid flowing into the quantitative tank is excessive.
7. According to the quantitative sample cup-type extraction device provided by the invention, the bottom of the quantitative tank is provided with the second liquid outlet hole, and the second liquid outlet hole is provided with the second sealing film; the reaction bin is suitable for being connected to the bottom of the filtering bin, and the top of the reaction bin is provided with a second puncture needle corresponding to the position of the second sealing film. The second sealing film and the second puncture needle are arranged, after the test sample is filtered and purified, the filter bin is placed on the reaction bin, and the purified test sample can flow into the reaction bin by puncturing the second sealing film through the second puncture needle; the device can not contact with the outside in the process of transferring the test sample, and ensures the tightness of the test sample and the accuracy of the subsequent detection result.
8. The quantitative sample cup-type extraction device provided by the invention is characterized in that a reaction tank suitable for storing reaction raw materials is arranged at the bottom of a reaction bin, the reaction tank is arranged corresponding to the quantitative tank in position, and a second puncture needle is arranged on the reaction tank. By the arrangement, the purified test sample can directly flow into the reaction tank to react with the reaction raw materials, and the contact with the outside in the flowing process can be avoided, so that the tightness of the test sample during the reaction is ensured.
9. The quantitative sample cup-in extraction device provided by the invention has the advantages that the energy storage bin is suitable for being connected to the bottom of the reaction bin; the top of the energy storage bin is provided with a heating structure, and a battery pack electrically connected with the heating structure is arranged inside the energy storage bin. The arrangement can directly carry out heating reaction on the test sample and the reaction raw materials in the reaction bin through the heating structure, and constant-temperature heating incubation is provided during the heating reaction, so that the accuracy of the reaction result is ensured; through setting up the battery package, can make extraction element still react under the circumstances of outage to this uninterrupted that guarantees experimental detection.
10. The invention provides a quantitative sample cup-in-cup extraction device, and a heating structure is a heating aluminum block. So set up, through the quick heat conductivility of aluminium material, improve the rate of heating to guarantee heating reaction efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a perspective view of a quantitative sample cup extraction device provided by the invention;
FIG. 2 is an exploded view of a device for extracting a quantitative sample in a cup according to the present invention;
FIG. 3 is a cross-sectional view of a device for extracting a quantitative sample in a cup according to the present invention;
fig. 4 is a perspective view of a filter cartridge according to the present invention.
Reference numerals illustrate: 1. a storage bin; 11. a vibrating structure; 12. a dilution tank; 13. sealing cover; 131. a buckle; 132. a connecting belt; 133. a push rod; 134. a silica gel sealing sleeve; 135. a compression cap; 136. a safety cover; 14. a first liquid outlet hole; 15. a first sealing film; 16. arc-shaped strip-shaped bulges; 2. a filtering bin; 21. a quantitative tank; 22. a first piercing needle; 23. a filtering structure; 24. a waste liquid tank; 25. waste liquid holes; 26. a second liquid outlet hole; 27. a second sealing film; 28. an arc-shaped groove; 29. a limiting ring; 210. a socket; 3. a reaction bin; 31. a reaction tank; 32. a second piercing needle; 33. clamping strips; 34. a limiting block; 35. a reaction raw material; 4. an energy storage bin; 41. a heating structure; 42. a battery pack; 43. a heating rod; 44. and (5) a control panel.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
A quantitative sample cup-in extraction device shown in figures 1-4 comprises a storage bin 1, a filtering bin 2, a reaction bin 3 and an energy storage bin 4; the storage bin 1 is adapted to store a diluent and a diluted test sample; the filter bin 2 is suitable for being communicated with the storage bin 1, and a filter structure 23 suitable for filtering a test sample is arranged on the filter bin 2; the reaction bin 3 is suitable for being communicated with the filtering bin 2, and the reaction bin 3 is suitable for storing reaction raw materials 35; the energy storage compartment 4 is adapted to provide heat to the reaction compartment 3 in order to react the reaction raw materials 35 with the test sample.
According to the quantitative sample cup-in-cup extraction device, the storage bin 1 is arranged for storing the diluent, the test sample is directly added when dilution is needed, and the diluent and the test sample are uniformly mixed in a shaking or vibrating mode to dilute the test sample; the filter bin 2 is communicated with the storage bin 1, the diluted test sample directly flows into the filter bin 2, and unnecessary sample components are filtered out when the test sample passes through the filter structure 23, so that the required sample components are separated; the reaction bin 3 is communicated with the filtering bin 2, the filtered test sample flows into the reaction bin 3, and the test sample and the reaction raw materials 35 in the reaction bin 3 can generate chemical reaction after the reaction bin 3 is heated by the energy storage bin 4. Through the cooperation in a plurality of structure warehouses, can carry out purification and the reaction of test sample, need not to reciprocate between a plurality of large-scale equipment, reduce the space occupation of equipment type and equipment, avoid the test sample to take place to contact with the external world in the purification reaction process, avoided the sample to be polluted, whole device is light and handy portable, operation convenient to use.
In the embodiment, the bin body shells of the storage bin 1, the filtering bin 2, the reaction bin 3 and the energy storage bin 4 are precisely injection molded in a 10-ten thousand GMP standard workshop, so that the quality standard of the shells can be ensured.
Specifically, when the storage bin 1 is connected with the filter bin 2, the lower end of the storage bin 1 is sleeved on the periphery of the upper end of the filter bin 2, a plurality of arc-shaped strip-shaped protrusions 16 which are uniformly arranged at intervals along the circumference are arranged on the inner wall of the lower end of the storage bin 1, and arc-shaped grooves 28 corresponding to the arc-shaped strip-shaped protrusions 16 are arranged on the outer wall of the upper end of the filter bin 2, so that the connection stability of the storage bin 1 and the filter bin 2 after being connected can be ensured. Specifically, a plurality of first notch grooves are uniformly formed in the circumferential direction on the side wall of the upper end of the filter bin 2, so that the smoothness of connection between the filter bin 2 and the storage bin 1 can be facilitated.
Specifically, when the filter bin 2 is connected with the reaction bin 3, the lower end of the filter bin 2 is sleeved on the periphery of the upper end of the reaction bin 3, a limiting ring 29 extending along the circumferential direction of the lower end of the filter bin 2 is arranged on the inner wall of the lower end of the filter bin 2, and a socket 210 is formed on the limiting ring 29; two opposite second notch grooves are formed in the side wall of the reaction bin 3, clamping strips 33 are arranged on the second notch grooves and are arranged at intervals with the side wall of the reaction bin 3, the upper ends of the clamping strips 33 are protruded out of the side wall of the reaction bin 3, limiting blocks 34 protruding out of the side wall of the clamping strips 33 are arranged on one side, far away from the reaction bin 3, of the upper ends of the clamping strips 33, and the limiting blocks 34 penetrate through the inserting holes 210 on the limiting rings 29 and then rotate the filter bin 2 or the reaction bin 3, so that the filter bin 2 and the reaction bin 3 can be firmly connected, and the connection stability of the filter bin 2 and the reaction bin 3 is effectively guaranteed.
In the present embodiment, a vibration structure 11, a dilution tank 12 located at one side of the vibration structure 11, and a sealing cover 13 covering the top of the dilution tank 12 are provided in the storage bin 1, and the dilution tank 12 is adapted to hold a dilution liquid. So arranged, the diluent can be stored in the diluent tank 12, and the test sample can be added by opening the sealing cover 13 when the test sample needs to be diluted; the diluent and the test sample in the diluting tank 12 can be uniformly mixed and diluted through the vibrating structure 11, so that the manual shaking is not needed, and the manpower can be effectively reduced when the number of the test samples to be processed is too large. Specifically, be provided with the power hole of being connected with ultrasonic motor electricity on the storage bin 1 lateral wall, can provide the electric energy for ultrasonic motor after the power is connected through the power hole. Specifically, the vibration structure 11 is an ultrasonic motor, and the ultrasonic motor is electrically connected with the bottom of the energy storage bin 4, and is suitable for providing electric energy for the ultrasonic motor through the bottom of the energy storage bin 4, so that the ultrasonic motor can be ensured to operate normally even when power is cut off.
In this embodiment, a first liquid outlet hole 14 is formed at the bottom of the dilution tank 12, and a first sealing film 15 is disposed on the first liquid outlet hole 14; the filter house 2 is adapted to be connected to the bottom of the storage house 1, the top of the filter house 2 being provided with a first piercing needle 22 corresponding to the position of the first sealing membrane 15. By arranging the first sealing film 15 and the first puncture needle 22 in this way, after the test sample is uniformly mixed and diluted, the storage bin 1 is placed on the filter bin 2, and the diluted test sample can flow into the filter bin 2 by puncturing the first sealing film 15 through the first puncture needle 22; the device can not contact with the outside in the process of transferring the test sample, and ensures the tightness of the test sample and the accuracy of the subsequent detection result. Specifically, the dilution tank 12 has a capacity of 400 microliters; the first sealing film 15 is a heat-seal aluminum film.
In this embodiment, the sealing cover 13 is specifically a pull-out sealing structure, and includes a buckle 131 fastened on the storage bin 1, a connection belt 132 integrally formed with the buckle 131, a push rod 133 mounted on the connection belt 132, a silica gel sealing sleeve 134 connected to the bottom end of the push rod 133, a compression cap 135 connected to the top of the push rod 133, and a safety cover 136 connected between the silica gel sealing sleeve 134 and the compression cap 135. So arranged, the sealing property of the sealing cover 13 covered on the storage bin 1 can be ensured through the silica gel sealing sleeve 134; by providing a safety cover 136 between the compression cap 135 and the silicone sealing sleeve 134, it is possible to avoid that the sealing cover 13 is separated from the storage bin 1 when being touched by mistake, thereby affecting the sealing performance thereof.
In this embodiment, a dosing tank 21 and a filter structure 23 located at the top of the dosing tank 21 are provided in the filter cartridge 2. This arrangement can be used to separate and filter the test sample before it enters the quantitative tank 21, thereby ensuring that the test sample flowing into the quantitative tank 21 is a filtered and purified test sample. Specifically, the filter structure 23 is a special blood filter.
In this embodiment, a waste liquid tank 24 is further provided in the filtration cartridge 2, which is located at the outer periphery of the quantitative tank 21, and a waste liquid hole 25 is provided between the waste liquid tank 24 and the quantitative tank 21, which is located at the top of the quantitative tank 21. In this way, when the amount of liquid flowing into the quantitative tank 21 is excessive, the liquid can be discharged into the waste liquid tank 24 through the waste liquid hole 25. Specifically, the capacity of the quantitative tank 21 is 100. Mu.l.
Specifically, the bottom of the quantitative tank 21 is provided with a second liquid outlet hole 26, and the second liquid outlet hole 26 is provided with a second sealing film 27; the reaction chamber 3 is adapted to be connected to the bottom of the filter chamber 2, and the top of the reaction chamber 3 is provided with a second piercing needle 32 corresponding to the position of the second sealing membrane 27. By arranging the second sealing film 27 and the second puncture needle 32 in this way, after the test sample is filtered and purified, the filter cartridge 2 is placed on the reaction cartridge 3, and the purified test sample can flow into the reaction cartridge 3 by puncturing the second sealing film 27 through the second puncture needle 32; the device can not contact with the outside in the process of transferring the test sample, and ensures the tightness of the test sample and the accuracy of the subsequent detection result.
In the present embodiment, a reaction tank 31 adapted to store a reaction raw material 35 is provided in the bottom of the reaction chamber 3, the reaction tank 31 is provided corresponding to the quantitative tank 21, and a second puncture needle 32 is provided in the reaction tank 31. By this arrangement, the purified test sample can directly flow into the reaction tank 31 to react with the reaction raw material 35, and the contact with the outside in the flowing process can not be generated, so that the tightness of the test sample in the reaction is ensured. Specifically, the bin body of the reaction bin 3 is formed by injection molding special high-permeability and freezing impact resistant polycarbonate materials, the reaction raw material 35 is placed in the reaction tank 31 in advance, and the second puncture needle 32 is welded at the top of the reaction tank 31 through ultrasonic waves, so that sealing is good. The reaction bin 3 with the reaction raw materials 35 can be directly stored in the refrigerator before unsealing for use, and can be taken out for use when an experimenter needs. Specifically, the first puncture needle 22 and the second puncture needle 32 are provided with a hole at the center.
In this embodiment, the energy storage bin 4 is adapted to be connected to the bottom of the reaction bin 3; the top of the energy storage bin 4 is provided with a heating structure 41, and a battery pack 42 electrically connected with the heating structure 41 is arranged inside the energy storage bin 4. So arranged, the test sample in the reaction bin 3 and the reaction raw materials 35 can be directly subjected to heating reaction through the heating structure 41, and constant-temperature heating incubation is provided during the heating reaction, so that the accuracy of the reaction result is ensured; by providing the battery pack 42, the extraction device can still react in the event of a power failure, thereby ensuring uninterrupted experimental detection. Specifically, the heating structure 41 is a heated aluminum block. So set up, through the quick heat conductivility of aluminium material, improve the rate of heating to guarantee heating reaction efficiency.
Specifically, the energy storage bin 4 is also provided with a heating rod 43 and a control board 44, the heating rod 43 is positioned below the heating aluminum block, and two ends of the heating rod 43 are respectively electrically connected with the battery pack 42 and the control board 44. The battery pack 42 provides constant temperature incubation at 37℃for the serum sample in the reaction chamber 3. Meanwhile, the battery pack 42 is arranged in the energy storage bottom bin, and the experimental test can be ensured not to be interrupted under the condition of power failure.
In the embodiment, the energy storage bin 4 is a reusable consumable material, and can be repeatedly used for a long time; the storage bin 1, the filtering bin 2 and the reaction bin 3 are all disposable consumables.
The using steps are as follows:
a first step of: opening the sealing cover 13, adding whole blood sample into the diluting tank 12 of the storage bin 1, covering the sealing cover 13, and uniformly mixing the whole blood sample and the diluent by ultrasonic vibration of an ultrasonic motor for 3-5min after connecting a power supply.
And a second step of: the storage bin 1 is buckled and pressed onto the filtering bin 2, the arc-shaped strip-shaped protrusions 16 are matched with the arc-shaped grooves 28 to fix the two bin bodies, the first puncture needle 22 punctures the heat-sealing aluminum film at the bottom of the diluting tank 12, the safety cover 136 is taken out, the push rod 133 is pressed down, and the diluted whole blood sample flows onto the special blood filtering film at the top of the quantifying tank 21 through the hole of the first puncture needle and then flows into the quantifying tank 21 through the special blood filtering film.
And a third step of: taking out the reaction bin 3 filled with the reaction raw materials 35 from the freezer, buckling the reaction bin 3 to the lower end of the serum quantitative filtering bin 2, puncturing the heat-sealing aluminum film at the bottom of the quantitative tank 21 by the second puncture needle 32, enabling the quantitative whole blood sample to flow into the reaction tank 31 at the bottom of the reaction bin 3 through the hole in the second puncture needle to react with the freeze-drying balls, and putting the combined body of the reaction bin 3 and the heat-sealing aluminum film on the heating rod 43/the heating aluminum block of the energy storage bottom bin for 15-20min for incubation.
Finally, the sample is put on an optical instrument for reading.
The quantitative sample cup-in-cup extraction device can concentrate the laboratory 10 steps to 3 steps, is simple and convenient to operate, and can effectively avoid secondary pollution.
In summary, the quantitative sample cup-in-cup extraction device is provided with the storage bin 1 for storing the diluent, the test sample is directly added when dilution is needed, and the diluent and the test sample are uniformly mixed by shaking or vibration to dilute the test sample; the filter bin 2 is communicated with the storage bin 1, the diluted test sample directly flows into the filter bin 2, and unnecessary sample components are filtered out when the test sample passes through the filter structure 23, so that the required sample components are separated; the reaction bin 3 is communicated with the filtering bin 2, the filtered test sample flows into the reaction bin 3, and the test sample and the reaction raw materials 35 in the reaction bin 3 can generate chemical reaction after the reaction bin 3 is heated by the energy storage bin 4. Through the cooperation in a plurality of structure warehouses, can carry out purification and the reaction of test sample, need not to reciprocate between a plurality of large-scale equipment, reduce the space occupation of equipment type and equipment, avoid the test sample to take place to contact with the external world in the purification reaction process, avoided the sample to be polluted, whole device is light and handy portable, operation convenient to use.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (10)
1. The quantitative sample cup-in-cup extraction device is characterized by comprising a storage bin (1), a filtering bin (2), a reaction bin (3) and an energy storage bin (4); the storage bin (1) is adapted to store a dilution liquid and a diluted test sample; the filter bin (2) is suitable for being communicated with the storage bin (1), and a filter structure (23) suitable for filtering a test sample is arranged on the filter bin (2); the reaction bin (3) is suitable for being communicated with the filtering bin (2), and the reaction bin (3) is suitable for storing reaction raw materials (35); the energy storage bin (4) is adapted to provide heat to the reaction bin (3) in order to react the reaction raw material (35) with a test sample.
2. The quantitative sample cup-in-cup extraction device according to claim 1, wherein a vibrating structure (11), a diluting tank (12) positioned at one side of the vibrating structure (11) and a sealing cover (13) arranged at the top of the diluting tank (12) in a covering manner are arranged in the storage bin (1), and the diluting tank (12) is suitable for storing diluent.
3. The quantitative sample cup-in-cup extraction device according to claim 2, wherein a first liquid outlet hole (14) is formed in the bottom of the dilution tank (12), and a first sealing film (15) is arranged on the first liquid outlet hole (14); the filter cartridge (2) is suitable for being connected to the bottom of the storage cartridge (1), and a first puncture needle (22) corresponding to the first sealing membrane (15) is arranged at the top of the filter cartridge (2).
4. The quantitative sample cup-in-cup extraction device according to claim 2, wherein the sealing cover (13) comprises a push rod (133) connected with the storage bin (1) through a buckle (131), a silica gel sealing sleeve (134) connected to the bottom end of the push rod (133), a pressing cap (135) connected to the top of the push rod (133), and a safety cover (136) connected between the silica gel sealing sleeve (134) and the pressing cap (135).
5. The quantitative sample cup-in-one extraction device according to claim 1, wherein a quantitative tank (21) and the filtering structure (23) positioned at the top of the quantitative tank (21) are arranged in the filtering bin (2).
6. The quantitative sample cup-in-cup extraction device according to claim 5, wherein a waste liquid tank (24) located at the periphery of the quantitative tank (21) is further provided in the filter cartridge (2), and a waste liquid hole (25) located at the top of the quantitative tank (21) is provided between the waste liquid tank (24) and the quantitative tank (21).
7. The quantitative sample cup-in-cup extraction device according to claim 5, wherein a second liquid outlet hole (26) is formed in the bottom of the quantitative tank (21), and a second sealing film (27) is formed on the second liquid outlet hole (26); the reaction bin (3) is suitable for being connected to the bottom of the filtering bin (2), and a second puncture needle (32) corresponding to the second sealing membrane (27) is arranged at the top of the reaction bin (3).
8. The quantitative sample cup-in-cup extraction device according to claim 7, wherein a reaction tank (31) adapted to store the reaction raw material (35) is provided in the bottom of the reaction chamber (3), the reaction tank (31) is provided corresponding to the quantitative tank (21), and the second piercing needle (32) is provided on the reaction tank (31).
9. The quantitative sample cupped extraction device according to claim 8, characterized in that the energy storage bin (4) is adapted to be connected to the bottom of the reaction bin (3); the top of the energy storage bin (4) is provided with a heating structure (41), and a battery pack (42) electrically connected with the heating structure (41) is arranged inside the energy storage bin (4).
10. A quantitative sample cup-in-device according to claim 9, wherein the heating structure (41) is a heated aluminium block.
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