CN216063326U

CN216063326U - Micro-fluidic chip

Info

Publication number: CN216063326U
Application number: CN202122672556.3U
Authority: CN
Inventors: 贺志民; 许明灶; 陈翀
Original assignee: Guangzhou Universal Junan Biotechnology Co ltd
Current assignee: Guangzhou Universal Junan Biotechnology Co ltd
Priority date: 2021-11-03
Filing date: 2021-11-03
Publication date: 2022-03-18
Anticipated expiration: 2031-11-03

Abstract

The utility model relates to a micro-fluidic chip, which comprises a chip board and a cap, wherein a reaction cavity and a sample cavity are arranged on the chip board, and the outlet end of the sample cavity can be communicated with the reaction cavity; the block is including the gasbag portion and the connecting portion that are connected, connecting portion can with the connection can be dismantled to the entry end of sample chamber, gasbag portion has initial condition and annotates the liquid state, and under the initial condition, gasbag portion is protruding towards the direction of keeping away from the sample chamber entry end, annotates under the liquid state, gasbag portion is sunken towards the direction of being close to the sample chamber entry end. The air bag part of the cap is pressed, the air bag part is sunken towards the direction close to the inlet end of the sample cavity, and due to the fact that air pressure is generated by the air bag part, liquid is pushed into the reaction cavity from the sample cavity, liquid injection operation can be completed without arranging an additional assembly, and cost is saved; the operation is simple, convenient and quick, the misoperation is reduced, and the risk generated in the using process is reduced.

Description

Micro-fluidic chip

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a micro-fluidic chip.

Background

The micro-fluidic chip can realize the processes of sample loading, reaction, detection and the like by combining with technologies such as biology, chemistry, medicines and the like. In order to realize that liquid such as a sample is pushed into a reaction cavity or a pretreatment cavity of a conventional microfluidic chip, the following methods are generally adopted: A. with the help of external auxiliary devices such as pipettes, additional pipettes are needed and leakage pollution is easily caused by non-closed state operation; B. driven by centrifugal force such as an injection pump and the like, but the instrument needs a centrifugal mechanism or an injection pump mechanism, and the cost is high; C. the internal mechanism of the chip, such as an air bag extrusion scheme in the chip, has high cost and low yield of the whole chip; or the push rod scheme is injected in the chip, but an operator is easy to push the push rod by an inaccurate distance, so that the volume of the next cavity is inaccurate; therefore, the method is difficult to adapt to the requirement of point-of-care testing (point-of-care testing) in the practical application scene of the medical industry.

SUMMERY OF THE UTILITY MODEL

Therefore, it is necessary to provide a microfluidic chip without external pipetting aid, which is simple to operate and low in cost, for the problems that an additional component is required to be configured in the conventional chip liquid injection process.

A microfluidic chip comprises

The chip plate is provided with a reaction cavity and a sample cavity, and the outlet end of the sample cavity can be communicated with the reaction cavity;

the block, the block is including the gasbag portion and the connecting portion that are connected, connecting portion can with the entry end in sample chamber can be dismantled and be connected, gasbag portion has initial condition and annotates the liquid state, and under the initial condition, gasbag portion is protruding towards the direction of keeping away from the sample chamber entry end, annotates under the liquid state, gasbag portion is sunken towards the direction of being close to the sample chamber entry end.

The microfluidic chip can be used for placing freeze-dried beads in a reaction cavity in advance, placing reagent liquid such as diluent in a sample cavity, sealing a chip plate through a cover cap, and enabling an air bag part to protrude towards the direction far away from the inlet end of the sample cavity and to be in an initial state; during the use, open the block cap up, then put into the sample chamber with the sample that awaits measuring, the block cap of screwing presses the gasbag portion of block cap again, and gasbag portion is sunken towards the direction that is close to the sample chamber entry end, is in annotates the liquid state, because gasbag portion produces air pressure, pushes liquid in the reaction chamber by the sample chamber, and the sample liquid that gets into the reaction chamber reacts with freeze-drying pearl. The microfluidic chip is connected with the cap with the air bag part on the sample cavity of the chip board, and the air bag part is pressed for one-step liquid injection, so that the operation can be simply and conveniently finished without configuring an additional component; parts are reduced, and the cost is saved; the use is more convenient, the misoperation is reduced, and the risk generated in the use process is reduced; and the liquid injection is operated in a closed state, so that the leakage pollution is reduced.

In one embodiment, the volume of the air bag part is the same as the volume of liquid required to be injected into the reaction cavity; and/or the cap is of a one-time injection molding structure.

In one embodiment, the chip board includes a body portion and a cavity portion fixed on the body portion, the reaction cavity is formed on the body portion, the sample cavity is formed in the cavity portion, the cavity portion is protruded from a first side surface of the body portion, and an inlet end of the cavity portion protrudes from an end edge of the body portion and is suspended.

In one embodiment, the microfluidic chip further comprises a sealing ring, the inlet end of the cavity part is cylindrical, the connecting part is cylindrical and matched with the inlet end of the cavity part, the connecting part is in threaded fit connection with the inlet end of the cavity part, the sealing ring is compressed between the connecting part and the inlet end of the cavity part, and one end, far away from the cavity part, of the connecting part is in sealing connection with the air bag part.

In one embodiment, the microfluidic chip further includes an on-off valve, the on-off valve includes a valve plug, a valve column and a valve body disposed on the body portion, the valve plug is disposed in the valve body, the valve column is rotatably disposed in the valve plug, and the valve column can be rotated to connect and disconnect the outlet end of the sample chamber and the reaction chamber.

In one embodiment, the valve body is disposed on the first side surface of the body, a first channel disposed along an extending direction of the cavity is disposed in the valve plug, one end of the first channel is communicated with the outlet end of the sample chamber, a second channel disposed along the extending direction of the valve body is disposed on the spool, a third channel disposed along the extending direction of the valve body is disposed on the body, one end of the third channel is communicated with the reaction chamber, and the spool rotates to enable the second channel to be communicated with and disconnected from the first channel and the third channel.

In one embodiment, a first positioning portion is arranged in the valve body, a second positioning portion matched with the first positioning portion is arranged on the valve plug, a third positioning portion is arranged on the valve body, and a fourth positioning portion matched with the third positioning portion is arranged on the valve column;

and/or the first positioning part is a raised line which is arranged on the inner wall of the valve body and arranged along the extending direction of the valve body, the second positioning part is a groove which is arranged on the outer wall of the valve plug and arranged along the extending direction of the valve plug, and the raised line is nested and matched with the groove;

and/or the third positioning part is a convex block which is arranged at the end part of the valve body and is arranged in a protruding manner, the valve column comprises a columnar part and an operation part which is arranged at one end of the columnar part, the second channel is arranged on the columnar part, the fourth positioning part is an arc-shaped clamping groove which is arranged on the operation part, the convex block is positioned in the arc-shaped clamping groove, when the valve column rotates to a first position, the first end of the arc-shaped clamping groove is in limit fit with the convex block, and the second channel is respectively communicated with the first channel and the third channel; when the valve column rotates to the second position, the second end of the arc-shaped clamping groove is in limit fit with the lug, and the second channel is not communicated with the first channel and the third channel.

In one embodiment, the microfluidic chip further includes a sealing film, the opening of the reaction chamber is disposed on the second side surface of the body, the reaction chamber is used for pre-placing lyophilized beads, the second side surface of the body is provided with a flow channel, the flow channel can be respectively communicated with the reaction chamber and the sample chamber, and the sealing film is attached to the second side surface of the body and seals the reaction chamber and the flow channel.

In one embodiment, the body part is further provided with an exhaust cavity, an outlet of the exhaust cavity is arranged on the first side surface, an inlet of the exhaust cavity is arranged on the second side surface, a filter plug is arranged in the exhaust cavity in advance, and the inlet of the exhaust cavity is communicated with the reaction cavity.

In one embodiment, the number of the reaction chambers is multiple, the number of the exhaust chambers is multiple, the reaction chambers and the exhaust chambers are arranged in a one-to-one correspondence manner, or the reaction chambers are at least communicated with one of the exhaust chambers; or the number of the reaction cavities is multiple, the number of the exhaust cavities is one, and the reaction cavities are communicated with the exhaust cavities;

the flow channel comprises a main path, a branch path and a flow dividing cavity, one end of the main path is communicated with the sample cavity, the other end of the main path is communicated with the flow dividing cavity, one end of the branch path is communicated with the reaction cavity, and the other end of the branch path is communicated with the flow dividing cavity.

Drawings

Fig. 1 is a schematic exploded view of a microfluidic chip according to an embodiment of the present application;

FIG. 2 is a schematic assembly view of the microfluidic chip of FIG. 1;

FIG. 3 is a schematic cross-sectional view of the microfluidic chip of FIG. 2 in an initial state;

FIG. 4 is a schematic cross-sectional view of the microfluidic chip of FIG. 2 in a liquid-filling state;

fig. 5 is a schematic view of a second side of the microfluidic chip of fig. 2.

10. A chip board; 102. a first side surface; 104. a second side surface; 110. a body portion; 112. a reaction chamber; 114. a flow channel; 1142. main path, 1144, branch path; 1146. a shunting cavity; 1148. an air exhaust path; 116. an exhaust chamber; 120. a cavity portion; 122. a sample chamber; 124. an inlet end; 20. capping; 210. an air bag portion; 220. a connecting portion; 30. freeze-drying the beads; 40. a seal ring; 50. an on-off valve; 510. a valve plug; 512. a first channel; 514. a second positioning portion; 520. a spool; 522. a second channel; 524. a fourth positioning portion; 530. a valve body; 532. a third channel; 534. a first positioning portion; 536. a third positioning part; 60. a sealing film; 70. and (5) filtering and plugging.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 to 4, a microfluidic chip according to an embodiment of the present invention includes a chip board 10 and a cap 20. The chip plate 10 is provided with a reaction chamber 112 and a sample chamber 122, and an outlet end of the sample chamber 122 can be communicated with the reaction chamber 112. The cap 20 includes a bladder portion 210 and a connecting portion 220 connected, the connecting portion 220 being removably connectable to the inlet end 124 of the sample chamber 122. The air bag portion 210 has an initial state and a liquid injection state, in the initial state, the air bag portion 210 is convex in a direction away from the inlet end 124 of the sample cavity 122; in the liquid-filled state, the balloon portion 210 is recessed toward the inlet end 124 of the sample chamber 122.

Placing a substance to be dissolved such as the lyophilized beads 30 in the reaction chamber 112 and a reagent solution such as a diluent in the sample chamber 122 in advance, and sealing the chip plate 10 by the cap 20, wherein the balloon portion 210 is protruded away from the inlet end 124 of the sample chamber 122 in an initial state; when the liquid injection device is used, the cap 20 is opened upwards, a sample to be tested is placed into the sample cavity 122, the cap 20 is screwed, the air bag portion 210 of the cap 20 is pressed, the air bag portion 210 is recessed towards the direction close to the inlet end 124 of the sample cavity 122 and is in a liquid injection state, air pressure is generated by the air bag portion 210, liquid is pushed into the reaction cavity 112 from the sample cavity 122, and the sample liquid entering the reaction cavity 112 reacts with the freeze-dried beads 30. The microfluidic chip is connected with the cap 20 with the air bag part 210 on the sample cavity 122 of the chip plate 10, liquid is injected in one step according to the air bag part 210, and the operation can be simply and conveniently finished without configuring additional components; parts are reduced, and the cost is saved; the use is more convenient, the stability is good, the liquid is accurately transferred, the misoperation is reduced, and the risk generated in the use process is reduced; and the liquid injection is operated in a closed state, so that the leakage pollution is reduced. The microfluidic chip is more suitable for a microfluidic chip kit, is used for quickly detecting biological collection samples, and can be used singly or used in combination with an instrument for multiple channels.

Preferably, the cap is of a one-time injection molding structure, and the cost is extremely low. Alternatively, the balloon portion 210 is made of a flexible deformable material, and the connecting portion 220 is made of a hard non-deformable material. The characteristics of the material are utilized to replace the original push rod pressure liquid pushing, the equivalent liquid injection effect is achieved, the parts are reduced, and the cost is saved.

Optionally, in one embodiment, the sample chamber 122 is used for holding a reagent solution or a sample-containing solution. The volume of the balloon portion 210 is the same as the volume of liquid required to be injected into the reaction chamber 112. Referring to fig. 3 and 4, the airbag portion 210 is convex hemispherical in the initial state, and is concave hemispherical in the liquid injection state, and the airbag portion is kept concave hemispherical in the liquid injection state by releasing the hand; the shape and volume of the balloon portion 210 are the same in the initial state and the liquid injection state, and thus the volume of liquid injected into the reaction chamber is fixed. The quantitative air bag part volume is converted into quantitative liquid transfer through compression, the rapid quantitative liquid injection operation can be realized, the liquid can be transferred to the reaction cavity simply and conveniently at low cost, the traditional liquid transfer device is not required to be operated or the external equipment such as a centrifugal machine and an injection pump is not required to be assisted, the original push rod range conversion liquid pushing and peristaltic pump liquid pushing are not required, and the liquid injection efficiency is higher. The sample is placed into a whole sealing operation for detection, so that leakage and cross contamination are avoided, and an additional component is not required to be matched for use; the operation is simple, and misoperation is avoided.

In one embodiment with reference to fig. 1, the microfluidic chip further includes a sealing ring 40, the inlet end 124 of the cavity portion 120 is cylindrical, the connecting portion 220 is cylindrical and is matched with the inlet end 124 of the cavity portion 120, the connecting portion 220 is in threaded fit connection with the inlet end 124 of the cavity portion 120, the sealing ring 40 is compressed between the connecting portion 220 and the inlet end 124 of the cavity portion 120, and one end of the connecting portion 220 away from the cavity portion 120 is in sealed connection with the air bag portion 210, so that the cap 20 and the chip board 10 are easy to detach and can be connected in a sealed manner. Optionally, in other embodiments, the connecting portion 220 and the inlet end 124 of the sample chamber 122 are detachably connected in a sealing manner by a bayonet structure.

Referring to fig. 1, in one embodiment, the chip board 10 includes a body 110 and a cavity 120 fixed on the body 110. Optionally, the body portion 110 and the cavity portion 120 are integrally formed. Referring to fig. 3 and 4, the reaction chamber 112 is formed on the body portion 110, and the sample chamber 122 is formed in the chamber portion 120. Optionally, the cavity portion 120 is projectively disposed on the first side surface 102 of the body portion 110. The body part 110 is plate-shaped; the cavity portion 120 has a tubular shape. The inlet end 124 of the chamber portion 120 protrudes from the end edge of the main body portion 110 and is suspended to facilitate the operation of detaching and connecting the cap 20 to the inlet end 124. Further, the sample chamber 122 extends along the length of the chip board 10; the reaction chamber 112 extends in the thickness direction of the chip plate 10. The reaction chamber 112 is disposed in the body portion 110 in an area away from the inlet end 124 of the chamber portion 120.

Referring to fig. 1-4, further, in one embodiment, the microfluidic chip further includes an on-off valve 50. The on-off valve 50 includes a valve plug 510, a spool 520, and a valve body 530 disposed on the body portion 110. The valve plug 510 is disposed in the valve body 530, the valve column 520 is rotatably disposed in the valve plug 510, and the rotation of the valve column 520 can connect and disconnect the outlet end of the sample chamber 122 and the reaction chamber 112. The communication between the sample chamber 122 and the reaction chamber 112 is controlled by controlling the rotational position of the spool 520 in the valve plug 510, the balloon portion 210 is pressed to allow the liquid in the sample chamber 122 to flow into the reaction chamber 112, the spool 520 is rotated to another position in the valve plug 510, the sample chamber 122 and the reaction chamber 112 are not communicated, and the reagent solution is temporarily stored in the sample chamber 122.

Further, in one embodiment, the valve body 530 is disposed on the first side 102 of the body 110. Optionally, the valve body 530 is integrally formed with the body 110. Referring to fig. 3 and 4, a first channel 512 is formed in the valve plug 510 and arranged along the extending direction of the cavity portion 120, one end of the first channel 512 is communicated with the outlet end of the sample chamber 122, a second channel 522 is formed in the valve rod 520 and arranged along the extending direction of the valve body 530, a third channel 532 is formed in the body portion 110 and arranged along the extending direction of the valve body 530, one end of the third channel 532 is communicated with the reaction chamber 112, and the rotation of the valve rod 520 can realize the communication and disconnection between the second channel 522 and the first channel 512 as well as between the third channel 532. As shown in fig. 3, the second channel 522 is disconnected from the first channel 512 and the third channel 532. As shown in fig. 4, the second passage 522 is in communication with the first passage 512 and the third passage 532. Pressing on bladder portion 210 causes fluid in sample chamber 122 to flow into reaction chamber 112 through first channel 512, second channel 522, and third channel 532, in the direction indicated by the arrows. Optionally, the first channel 512 and the third channel 532 are arranged perpendicular to each other. Referring to FIG. 1, the second passage 522 is a slotted structure extending through the bottom end and the outer wall of the spool 520.

Further, a first positioning portion is disposed in the valve body 530, and a second positioning portion 514 matched with the first positioning portion is disposed on the valve plug 510. The first positioning portion and the second positioning portion 514 cooperate to enable the valve plug 510 to be quickly positioned and installed in the valve body 530. The valve body 530 is provided with a third positioning portion, and the spool 520 is provided with a fourth positioning portion matched with the third positioning portion. The third positioning portion and the fourth positioning portion are matched, so that the valve column 520 can be quickly positioned and installed in the valve plug 510.

Referring to fig. 1, 3 and 4, specifically, in one embodiment, the first fixing portion is a protruding strip disposed on an inner wall of the valve body 530 and disposed along an extending direction of the valve body 530, and the second fixing portion 514 is a groove disposed on an outer wall of the valve plug 510 and disposed along the extending direction of the valve plug 510, and the protruding strip is nested and matched with the groove. In other embodiments, the first fixing portion is a groove disposed on an inner wall of the valve body 530 and extending along an extending direction of the valve body 530, and the second fixing portion 514 is a protrusion disposed on an outer wall of the valve plug 510 and extending along the extending direction of the valve plug 510, and the protrusion is nested with the groove. By nesting the ribs and grooves, the valve plug 510 can be quickly positioned in the valve body 530 such that the first channel 512 is in abutting communication with the outlet end of the sample chamber 122.

Referring to fig. 1, 3 and 4, specifically, in one embodiment, the third positioning portion is a protruding block that is disposed at an end of the valve body 530 and protrudes. The spool 520 includes a cylindrical portion and an operating portion disposed at one end of the cylindrical portion, and the second passage 522 is opened in the cylindrical portion. The fourth positioning part is an arc-shaped clamping groove formed in the operating part, and the convex block is located in the arc-shaped clamping groove. Referring to fig. 4, when the spool 520 rotates to the first position, the first end of the arc-shaped clamping groove is in limit fit with the protruding block, the second channel 522 is respectively communicated with the first channel 512 and the third channel 532, and liquid injection can be realized by pressing the air bag portion 210 at this time. Referring to fig. 3, when the spool 520 rotates to the second position, the second end of the arc-shaped slot is in limit fit with the protrusion, the second channel 522 is not communicated with the first channel 512 and the third channel 532, the balloon portion 210 protrudes outward, and the reagent solution is stored in the sample chamber 122.

Further, in one embodiment, the microfluidic chip further includes a sealing film 60. The opening of the reaction chamber 112 is opened at the second side surface 104 of the body portion 110. The reaction chamber 112 is used for pre-placing the freeze-dried beads 30. The second side surface 104 of the body 110 defines a flow channel 114, the flow channel 114 is capable of being respectively communicated with the reaction chamber 112 and the sample chamber 122, and the sealing film 60 is attached to the second side surface 104 of the body 110 and seals the reaction chamber 112 and the flow channel 114. The body portion 110 is internally sealed by sealing the membrane 60 to the second side 104 of the body portion 110. Optionally, the sealing film 60 is made of high-grade synthetic glue, is resistant to strong acid and strong alkali, has good sealing performance, is free of gum and residue, does not react with a reagent solution or a sample, and avoids inaccuracy of detection data.

Referring to fig. 1-5, further, in one embodiment, the body 110 is further provided with a venting chamber 116. The outlet of the exhaust cavity 116 is open at the first side 102 and the inlet of the exhaust cavity 116 is open at the second side 104. The vent cavity 116 is used for pre-arranging the filter plug 70, and the inlet of the vent cavity 116 is communicated with the reaction cavity 112. When pressing gasbag portion 210 and annotating the liquid, reentrant exhaust chamber 116 after reaction chamber 112 is filled up to the liquid in the sample chamber 122, and the liquid uniflow in-process is discharged the bubble of inside production to exhaust chamber 116 in, and gaseous export through exhaust chamber 116 is discharged, avoids the bubble to the influence of testing result in the reaction chamber 112, and filter plug 70 can exhaust gas, avoids liquid to flow out by the export of exhaust chamber 116.

Optionally, in one embodiment, there are a plurality of reaction chambers 112, a plurality of exhaust chambers 116, and the reaction chambers 112 and the exhaust chambers 116 are disposed in a one-to-one correspondence. By adopting a plurality of reaction chambers 112 and an exhaust chamber 116, the sample liquid can enter different reaction chambers 112 respectively, and can realize detection of different items or recheck detection of a certain item in different reaction chambers 112 at the same time, thereby improving the detection efficiency and the accuracy of the detection result, and solving the problems of few or single detection reading of the reaction chambers 112 of the traditional chip and accidental result of the detection data. Optionally, in other embodiments, there are a plurality of reaction chambers 112, a plurality of exhaust chambers 116, and the reaction chamber 112 is in communication with at least one of the exhaust chambers 116; or a plurality of reaction chambers 112 are provided, one exhaust chamber 116 is provided, and each reaction chamber 112 is communicated with the exhaust chamber 116.

Referring to fig. 5, further, the flow channel 114 includes a main path 1142, a branch path 1144 and a branch flow cavity 1146, one end of the main path 1142 is communicated with the sample cavity 122, the other end of the main path 1142 is communicated with the branch flow cavity 1146, one end of the branch flow cavity 1144 is communicated with the reaction cavity 112, and the other end of the branch flow cavity 1144 is communicated with the branch flow cavity 1146. The liquid in the sample chamber 122 enters the branch flow chamber 1146 through the main path 1142, and then flows into each branch path 1144 through the branch flow chamber 1146, and further enters each reaction chamber 112, and each reaction chamber 112 is filled in sequence respectively until all the reaction chambers 112 are just filled, so as to avoid the situation that some reaction chambers 112 are full and some reaction chambers 112 are not full, and realize the equal flow distribution of each reaction chamber 112. The back of the liquid flowing out direction in the reaction cavity 112 is communicated with an exhaust cavity 116 with an outlet, a filter plug 70 is arranged in the exhaust cavity 116, the gas in the reaction cavity 112 is easy to exhaust, even if a small amount of residual bubbles are gathered near the exhaust cavity 116, and the influence on the detection result in the reaction cavity 112 is small.

Further, the reaction chamber 112 communicates with the exhaust chamber 116 through an exhaust path 1148. When signal detection (especially optical signals) is carried out, excessive retained bubbles can influence reaction (uniform mixing, surface tension and heat conduction influence) and signal detection (interfered, refracted and scattered influence signal collection). The injection and exhaust of each branch 1144 and the exhaust path 1148 flow in a single direction, and the molecular bubbles generated during the chip injection and heating process are gathered near the exhaust cavity 116, thereby avoiding the influence on the fluorescence collection of the reaction cavity 112. Referring to fig. 5, the microfluidic chip is vertically placed during liquid injection, the sample liquid enters the liquid separation chamber through the main path, and then each reaction chamber 112 is sequentially filled with each branch located at the periphery of the liquid separation chamber, and then enters the exhaust chamber 116 through the exhaust path 1148. Wherein the outlet of the branch is located at the lower end of the reaction chamber 112 and the inlet of the exhaust path 1148 is located at the upper end of the reaction chamber 112.

Referring to FIG. 5, in one embodiment, the flow splitting cavity 1146 is a circular cavity, which facilitates better uniform flow distribution among the reaction cavities 112. Optionally, the branch 1144 is a curved path forming a resistive curve to keep each reaction chamber 112 liquid full while avoiding cross contamination of the heating cross-flow. In other embodiments, the branch 1144 may be a straight track or other irregular tracks. Furthermore, the connection between the split-flow cavity 1146 and the reaction cavity 112 is rounded to facilitate gas exhaust.

Referring to fig. 1, the micro-fluidic chip works as follows: placing the freeze-dried ball and the filter plug 70 into the reaction chamber 112 and the exhaust chamber 116, respectively, attaching a sealing film 6060, placing the valve plug 510 into the valve body 530 for clamping, inserting the valve column 520 into the valve plug 510, and keeping the valve column in a closed state; then, a quantitative reagent liquid is added into the sample cavity 122, the sample cavity is tightly screwed and sealed by the cap 20 with the sealing ring 4040, and the sample cavity is placed into an ultraviolet-proof rubber bag for vacuum pumping and sealing. And opening the package to take out the microfluidic chip, opening the cap 20 upwards, putting the to-be-detected swab sample into the sample cavity 122, rotating for 3-5 circles, then breaking, screwing the cap 20, opening the valve posts 520, pressing the air bag part 210 at the top of the cap 20, pushing the sample liquid into each reaction cavity 112 through the flow channels 114 by the air pressure generated by the air bag part 210, and closing the valve posts 520 after the reaction cavities 112 are filled with the sample liquid to finish the operation.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A microfluidic chip is characterized by comprising

2. The microfluidic chip according to claim 1, wherein the volume of the air bag part is the same as the volume of the liquid required to be injected into the reaction chamber; and/or the cap is of a one-time injection molding structure.

3. The microfluidic chip according to claim 1, wherein the chip board includes a body portion and a cavity portion fixed on the body portion, the reaction chamber is formed on the body portion, the sample chamber is formed in the cavity portion, the cavity portion is protruded from a first side of the body portion, and an inlet end of the cavity portion protrudes from an end edge of the body portion and is suspended therefrom.

4. The microfluidic chip according to claim 3, further comprising a sealing ring, wherein the inlet end of the chamber portion is cylindrical, the connecting portion is cylindrical and is matched with the inlet end of the chamber portion, the connecting portion is in threaded fit with the inlet end of the chamber portion, the sealing ring is compressed between the connecting portion and the inlet end of the chamber portion, and one end of the connecting portion, which is far away from the chamber portion, is in sealed connection with the air bag portion.

5. The microfluidic chip according to any of claims 3 to 4, further comprising an on-off valve, wherein the on-off valve comprises a valve plug, a valve post and a valve body disposed on the body portion, the valve plug is disposed in the valve body, the valve post is rotatably disposed in the valve plug, and the rotation of the valve post can connect and disconnect the outlet end of the sample chamber and the reaction chamber.

6. The microfluidic chip according to claim 5, wherein the valve body is disposed on the first side surface of the body, a first channel is disposed in the valve plug along an extending direction of the body, one end of the first channel is connected to the outlet of the sample chamber, a second channel is disposed in the valve plug along the extending direction of the valve body, a third channel is disposed in the body along the extending direction of the valve body, one end of the third channel is connected to the reaction chamber, and rotation of the valve plug enables connection and disconnection between the second channel and the first and third channels.

7. The microfluidic chip according to claim 6, wherein a first positioning portion is disposed in the valve body, a second positioning portion matching with the first positioning portion is disposed on the valve plug, a third positioning portion is disposed on the valve body, and a fourth positioning portion matching with the third positioning portion is disposed on the valve post;

8. The microfluidic chip according to any one of claims 3 to 4, further comprising a sealing film, wherein the opening of the reaction chamber is disposed on the second side surface of the body, the reaction chamber is configured to store the lyophilized beads therein, the second side surface of the body is configured with a flow channel, the flow channel is capable of being respectively communicated with the reaction chamber and the sample chamber, and the sealing film is attached to the second side surface of the body and seals the reaction chamber and the flow channel.

9. The microfluidic chip according to claim 8, wherein the body further comprises an exhaust cavity, an outlet of the exhaust cavity is disposed on the first side surface, an inlet of the exhaust cavity is disposed on the second side surface, a filter plug is disposed in the exhaust cavity, and the inlet of the exhaust cavity is communicated with the reaction cavity.

10. The microfluidic chip according to claim 9, wherein the number of the reaction chambers is multiple, the number of the exhaust chambers is multiple, the reaction chambers and the exhaust chambers are arranged in a one-to-one correspondence, or the reaction chambers are communicated with at least one of the exhaust chambers; or the number of the reaction cavities is multiple, the number of the exhaust cavities is one, and the reaction cavities are communicated with the exhaust cavities;