CN221192145U - Integrated quantitative reaction tube - Google Patents
Integrated quantitative reaction tube Download PDFInfo
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- CN221192145U CN221192145U CN202322928631.7U CN202322928631U CN221192145U CN 221192145 U CN221192145 U CN 221192145U CN 202322928631 U CN202322928631 U CN 202322928631U CN 221192145 U CN221192145 U CN 221192145U
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- reaction tube
- liquid storage
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- storage chamber
- reagent
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- 239000007788 liquid Substances 0.000 claims abstract description 86
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 51
- 238000005192 partition Methods 0.000 claims description 23
- 238000003825 pressing Methods 0.000 claims description 19
- 238000007789 sealing Methods 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 15
- 238000005070 sampling Methods 0.000 abstract description 9
- 238000004458 analytical method Methods 0.000 abstract description 6
- 230000010354 integration Effects 0.000 abstract description 3
- 230000003321 amplification Effects 0.000 description 11
- 238000003199 nucleic acid amplification method Methods 0.000 description 11
- 108020004707 nucleic acids Proteins 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 238000013461 design Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 108091033409 CRISPR Proteins 0.000 description 2
- 238000010354 CRISPR gene editing Methods 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 239000007888 film coating Substances 0.000 description 2
- 238000009501 film coating Methods 0.000 description 2
- 238000011901 isothermal amplification Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses an integrated quantitative reaction tube, which comprises: the quantitative assembly is used for quantitatively collecting the liquid in the reaction tube; a liquid storage part having at least one liquid storage chamber for presetting a reagent; the reaction tube is used for mixing and reacting the sample and the reagent; and the functional part is used for releasing the preset reagent in the liquid storage chamber into the reaction tube. At least one reagent is preset in the liquid storage chamber, when the liquid storage chamber is used, a sample is placed in the reaction tube, then the functional part is operated, the reagent in the liquid storage chamber is released into the reaction tube to be mixed and reacted with the sample, and then the quantitative mixed liquid can be sucked by the quantitative component for subsequent detection and analysis. The utility model organically integrates the quantitative sampling pipette, the reagent and the reaction tube, has simple structure, convenient and quick use, can be operated and used even by non-professional staff, and has the advantages of high integration level and easy use.
Description
Technical Field
The utility model relates to the field of medical instruments, in particular to an integrated quantitative reaction tube.
Background
In the field of in vitro diagnostic analysis, it is often necessary to quantitatively draw a trace amount of liquid into an analysis system, and in the prior art, there is a sampling tube having a quantitative pipette and a redundant cartridge, for example, a sampling tube disclosed in CN202222054717.7, in which a portion exceeding the capacity of the pipette can be sucked at one time, and a portion exceeding the capacity of the pipette can flow into the redundant cartridge, and the liquid in the pipette is a liquid with a certain capacity, so that simple and efficient quantitative sampling is realized. However, in the prior art, the sampling tube has only a pipetting function, a mixed reaction function in a plurality of reagent stages is not stored in the tube, and an additional reaction test tube, a reagent kit and the like are required to be matched for use, and the current application demands hope that an integrated reaction tube for realizing sampling and reaction rapidly and conveniently.
Disclosure of utility model
The utility model provides an integrated quantitative reaction tube, which overcomes the defects of the prior art and realizes the precise quantitative stage mixing reaction of various reagents.
The utility model provides an integrated quantitative reaction tube, comprising:
The quantitative assembly is used for quantitatively collecting the liquid in the reaction tube;
A liquid storage part having at least one liquid storage chamber for presetting a reagent;
the reaction tube is used for mixing and reacting the sample and the reagent;
And the functional part is used for releasing the preset reagent in the liquid storage chamber into the reaction tube.
At least one reagent is preset in the liquid storage chamber (the quantity of the liquid storage chambers is determined), when the liquid storage chamber is used, a sample is placed in the reaction tube, then the functional part is operated, the reagent in the liquid storage chamber is released into the reaction tube to be mixed and reacted with the sample, and then the quantitative mixed liquid can be sucked by the quantitative component for subsequent detection and analysis. The utility model organically integrates the quantitative sampling pipette, the reagent and the reaction tube, has simple structure, convenient and quick use, can be operated and used even by non-professional staff, and has the advantages of high integration level and easy use.
Further, the quantitative component, the liquid storage part, the functional part and the reaction tube are sequentially connected together from top to bottom.
Further, the quantitative assembly comprises a pressing part and a pipette, the pressing part comprises a main body and a pressing air bag, the main body is of a cup-shaped structure with an opening at the upper end, the pressing air bag is installed at the opening of the main body in a sealing mode, the upper portion of the pipette penetrates through the bottom of the main body and extends into a cavity formed by the cup-shaped structure of the main body and the pressing air bag together, and the lower portion of the pipette penetrates through the functional part and extends into the reaction tube.
Further, the bottom of the main body is integrally provided with the liquid storage part, the liquid storage chamber is provided with a downward opening, and the functional part can rotate relative to the quantitative assembly, so that the preset reagent in the liquid storage chamber is kept in the liquid storage chamber or released into the reaction tube at different angle positions.
Further, the functional part is provided with an annular body, a functional partition board is arranged in the middle of the annular body, a plurality of through holes are formed in the functional partition board, when the functional part is arranged at some angle positions relative to the quantitative assembly, the solid part of the functional partition board seals the opening of the liquid storage chamber, and when the functional part is arranged at other angle positions, the through holes correspond to the opening of the liquid storage chamber, so that reagents in the liquid storage chamber flow into the reaction tube through the through holes.
Further, a guide buckle is arranged on the outer wall of the main body, a screwing locating groove is formed in the inner wall of the annular body and located above the functional partition plate, and the guide buckle is matched with the screwing locating groove and can slide along the screwing locating groove.
Further, the outer wall of the main body is positioned above the guide buckle, and the inner wall of the annular body is positioned above the functional partition plate and provided with one-way teeth which are mutually matched.
Further, be equipped with the pipette through-hole that supplies the pipette to pass on the function baffle, be equipped with the pipette sealing washer with the sealed adaptation of pipette through-hole on the pipette.
Furthermore, a liquid storage chamber sealing ring is arranged at the opening of the liquid storage chamber.
Further, the main body is provided with a first connecting part connected with the functional part, the upper part of the functional part is provided with a second connecting part matched with the first connecting part, the lower part of the functional part is provided with a third connecting part connected with the reaction tube, and the upper part of the reaction tube is provided with a fourth connecting part matched with the third connecting part.
Drawings
In order to make the technical solution of the present application better understood by those skilled in the art, the technical solution of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, shall fall within the scope of the application.
FIGS. 1 and 2 are schematic structural views of an integrated quantitative reaction tube according to the present utility model;
fig. 3 and 4 are schematic structural views of the functional section;
Fig. 5 is a schematic view of an integrally formed structure of a dosing assembly and a reservoir.
Wherein the identification of each part is as follows:
A dosing assembly 1; a pipette seal ring 11; a reservoir seal ring 12; a guide buckle 13; unidirectional teeth 14; pressing the air bag 15; a liquid storage chamber 16; a pipette 17; an annular groove 18; a functional unit 2; screwing the positioning groove 21; a guide groove 22; a pipette through hole 23; a through hole 24; a functional separator 25; and a reaction tube 3.
Detailed Description
In order to make the technical solution of the present application better understood by those skilled in the art, the technical solution of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, shall fall within the scope of the application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
As shown in fig. 1 to 5, an integrated quantitative reaction tube, comprising:
A dosing assembly 1 for dosing a liquid;
A reservoir having at least one reservoir chamber 16 for pre-setting a reagent; in the example of fig. 1, there are two reservoirs;
a reaction tube 3 for mixing the sample and the reagent;
a functional part 2 for releasing the preset reagent in the liquid storage chamber into the reaction tube.
At least one reagent is preset in the liquid storage chamber (the quantity of the liquid storage chambers is determined), when the liquid storage chamber is used, a sample is placed in the reaction tube, then the functional part is operated, the reagent in the liquid storage chamber is released into the reaction tube to be mixed and reacted with the sample, and then the quantitative mixed liquid can be sucked by the quantitative component for subsequent detection and analysis. The utility model organically integrates the quantitative sampling pipette, the reagent and the reaction tube, has simple structure, convenient and quick use, can be operated and used even by non-professional staff, and has the advantages of high integration level and easy use.
In the example of fig. 1-5, the dosing assembly 1, the reservoir, the functional part 2 and the reaction tube 3 are connected together in sequence from top to bottom. In the above example, the liquid reservoir and the dosing assembly 1 are integrally formed together, and the structure can be simplified.
As shown in fig. 1, 2 and 5, the dosing assembly 1 comprises a pressing part and a pipette 17, the pressing part comprises a main body and a pressing air bag 15, the main body is a cup-shaped structure with an open upper end, the pressing air bag 15 is installed at the open position of the main body in a sealing mode, the upper part of the pipette 17 penetrates through the bottom of the main body and extends into a cavity formed by the cup-shaped structure of the main body and the pressing air bag 15, and the lower part of the pipette 17 penetrates through the functional part 2 and extends into the reaction tube 3. The pressing air bag can be integrally formed with the main body, and the pressing air bag part is made of a flexible material with shape recovery characteristics.
In the above example, the bottom of the body is integrally provided with the reservoir, the reservoir being two reservoirs 16, the reservoirs 16 having a downward opening, the functional part 2 being rotatable relative to the dosing assembly 1, which in different angular positions enables the retention of reagents preset in the reservoirs 16 in the reservoirs or the release into the reaction tube 3. The two liquid storage chambers 16 illustrated in the figure have different shapes, one is a cylindrical cavity and one is an elliptic cylindrical cavity, so that different actual presettings in different liquid storage chambers can be distinguished, the design is similar to a foolproof design and is not easy to make mistakes, when different reagents are required to be released in sequence, the design can prevent the preset mistakes of the reagents, in addition, the liquid storage chamber 16 is processed in a determined main cylindrical bottom space, and the volume of the elliptic cylindrical liquid storage chamber is larger than that of the cylindrical liquid storage chamber, so that the requirement of storing the reagents with different capacities can be met. An annular groove 18 for placing the liquid storage chamber sealing ring 12 is arranged at the opening of the liquid storage chamber 16, and is used for placing the liquid storage chamber sealing ring 12 to enable the liquid storage chamber to form a sealed cavity under the matching of the liquid storage chamber sealing ring 12 and the functional partition 25 of the functional part 2.
In some embodiments, the functional part 2 has an annular body, and a functional partition 25 is disposed at the middle or bottom of the annular body, and a plurality of through holes 24 (generally the same as the number of the liquid storage chambers 16) are disposed on the functional partition 25, so that when the functional part 2 is at some angular positions relative to the dosing assembly 1, the functional partition 25 seals the opening of the liquid storage chamber 16 in a solid manner, and when at other angular positions, the through holes 24 correspond to the opening of the liquid storage chamber 16, so that the reagent in the liquid storage chamber 16 flows into the reaction tube 3 through the through holes 24. The plurality of liquid storage chambers 16 are circumferentially arranged along the circle center at the bottom of the main body, the angles of the liquid storage chambers 16 are different, the positions and angles of different through holes 24 on the functional part 2 are matched, and when the functional part is rotated to a certain angle, part or all of the liquid storage chambers 16 are aligned with the corresponding through holes 24, so that the reagent in different liquid storage chambers 16 is released simultaneously, or the reagent in different liquid storage chambers 16 is released sequentially at different rotation angles.
In order to realize the relative rotation between the functional part 2 and the dosing assembly 1, a guiding buckle 13 (two rectangular protrusions which are oppositely arranged in the example in the figure) is arranged on the outer wall of the main body, a screwing positioning groove 21 is arranged on the inner wall of the annular body and above the functional partition plate 25, and the guiding buckle 13 is matched with the screwing positioning groove 21 and can slide along the screwing positioning groove 21. In order to facilitate the insertion of the guide buckle 13 into the screwing positioning groove 21, a vertical guide groove 22 is formed from the upper edge of the inner wall of the annular body to the beginning of the screwing positioning groove 21, the depth of the guide groove 22 can be shallower than that of the screwing positioning groove 21, so that when the guide buckle 13 reaches the screwing positioning groove 21 through the guide groove 22, the depth difference between the two grooves is limited, the guide buckle is not easy to fall off, and meanwhile, the liquid storage chamber 16 can obtain a reliable sealing effect.
In order to define that the functional part 2 can only be rotated quantitatively in one direction relative to the dosing assembly 1 (which facilitates the sequential release of different reagents in different reservoirs 16), the outer wall of the body is located above the guide button and the inner wall of the annular body is provided with mutually adapted unidirectional teeth 14 located above the functional partition.
For the dosing assembly 1, the storage part (in the illustrated example and the dosing assembly are integrally formed, or can be independently prepared), the functional part 2 and the reaction tube 3 can be connected into a whole, the functional partition 25 is provided with a pipette through hole 23 for a pipette 17 to pass through, and the pipette 17 is provided with a pipette sealing ring 11 which is in sealing fit with the pipette through hole 23. The reaction tube 3 is sealed into an independent cavity by the functional partition 25 by the pipette sealing ring 11, after the sample is collected and put into the reaction tube 3, the quantitative component 1 and the functional part 2 are connected together (the functional part 2 and the reaction tube 3 are in threaded connection in the drawing), then the sealed storage can be realized for standby (whether the functional part 2 can be immediately rotated to release the reagent according to the requirement), while the prior art needs an additional reaction tube and a cap thereof, a reagent container, a quantitative pipette and the like, and the sample is various, and the operation and the management are very troublesome and inconvenient.
In order to connect each part in series into a whole in sequence, the main body is provided with a first connecting part connected with the functional part, the upper part of the functional part is provided with a second connecting part matched with the first connecting part, the lower part of the functional part is provided with a third connecting part connected with the reaction tube, and the upper part of the reaction tube is provided with a fourth connecting part matched with the third connecting part. In the illustrated example, the first connecting portion and the second connecting portion respectively adopt a guide buckle 13 and a screwing positioning groove 21, and the third connecting portion and the fourth connecting portion adopt external threads and internal threads which are mutually in threaded fit, and can also adopt other feasible connecting structures, which are not described again.
When the quantitative liquid storage device is specifically used, firstly, a storage part or a quantitative component containing the storage part is integrally formed in an illustrated example, the quantitative liquid storage device is turned upwards, required quantitative reagents are respectively placed into different liquid storage chambers, then the functional part is inverted and assembled with the quantitative component, sealing of the liquid storage chambers is achieved, then the reaction pipes are connected together or not connected together for disinfection and encapsulation, the clinical application is that the encapsulation is removed, the reaction pipes are taken out, sampling samples are placed into the reaction pipes, then the reaction pipes and the functional part are mutually screwed, the functional part and the quantitative component are relatively rotated, the reagents are released into the reaction pipes, the pressing air bags are pressed when needed, quantitative reaction mixed liquid is sucked into the pipette (related patents related to the working principle of the quantitative pipette), then the quantitative component (can be together with the functional part), and the quantitative mixed liquid in the pipette is used in subsequent operation. The pipette can also be used for quantitatively sucking liquid from the outside and putting the liquid into the reaction tube, and reasonable utilization is carried out according to the actual experimental needs, so that compared with the prior art, the pipette is more convenient and quick.
Of course, the liquid storage chamber can also be sealed by adopting a film-coating mode, and then the functional partition plate of the functional part is required to be provided with corresponding protruding thorns, so that when the functional part is rotated to a preset angle, the protruding thorns puncture the film-coating, and the reagent flows into the reaction tube from the through holes on the functional partition plate.
In some embodiments, a vertical partition plate is arranged inside a reaction tube of the integrated quantitative reaction tube, the reaction tube is internally divided into a reaction cavity and a detection cavity, test strips are arranged in the detection cavity, after the mixed liquid in the reaction cavity is reacted, the reaction tube is inverted, and the reacted mixed liquid enters the detection cavity through a notch or a through hole at the upper end of the partition plate to contact the test strips, so that the mixed reaction liquid obtained in the reaction tube can be directly detected.
The method for using the integrated quantitative reaction tube is described below, and includes:
Placing a nucleic acid amplification reagent in the reaction tube 3, and placing a functional reaction reagent in the liquid storage part;
The integrated quantitative reaction tube is inserted into an incubator to control the temperature, and only the chamber in which the nucleic acid amplification reagent is placed is subjected to contact conduction heating; when the nucleic acid amplification reaction is completed, the functional reagent is mixed with the amplification product that completes the amplification reaction.
Specifically, when the nucleic acid amplification reaction is completed, the integrated quantitative reaction tube is removed from the incubator, and the functional part of the integrated quantitative reaction tube is rotated to mix the functional reaction reagent with the amplification product that completes the amplification reaction.
The reaction tube can be directly contacted with the detection device for detection, or the liquid in the reaction tube is quantitatively transferred into any detection device for detection through the quantitative component of the integrated quantitative reaction tube.
It may also include moving the sample into the reaction tube through the quantification assembly, contacting with a nucleic acid amplification reagent, and initiating a subsequent amplification reaction.
The following experiments are taken as examples, and include:
placing a nucleic acid isothermal amplification reagent into a reaction tube, and placing a CRISPR detection reagent into a liquid storage chamber;
The integrated quantitative reaction tube is inserted into an incubator or a PCR instrument for amplifying nucleic acid to control the temperature, only the solution in the reaction tube is conducted by the temperature, and the liquid storage part is not contacted with the heat transfer component;
After the nucleic acid amplification reaction is finished, the integrated quantitative reaction tube is pulled out, and the functional part of the integrated quantitative reaction tube is rotated, so that the CRISPR reagent in the liquid storage chamber enters the reaction tube to be mixed with the nucleic acid isothermal amplification reagent, and the mixture is put into fluorescent detection equipment for analysis of detection results.
The liquid in the reaction tube can be quantitatively transferred into any detection device for detection through the quantitative component of the integrated quantitative reaction tube.
It is to be understood that the above description is intended to be illustrative, and not restrictive. Many embodiments and many applications other than the examples provided will be apparent to those of skill in the art upon reading the above description. The scope of the present teachings should, therefore, be determined not with reference to the above description, but instead should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled. The disclosures of all articles and references, including patent applications and publications, are incorporated herein by reference for the purpose of completeness. The omission of any aspect of the subject matter disclosed herein in the preceding claims is not intended to forego such subject matter, nor should the applicant be deemed to have such subject matter not considered to be part of the subject matter of the disclosed application.
Claims (10)
1. An integrated quantitative reaction tube, comprising:
the quantitative assembly is used for quantitatively collecting liquid;
A liquid storage part having at least one liquid storage chamber for presetting a reagent;
the reaction tube is used for mixing and reacting the sample and the reagent;
And the functional part is used for releasing the preset reagent in the liquid storage chamber into the reaction tube.
2. The integrated quantitative reaction tube according to claim 1, wherein the quantitative component, the liquid storage part, the functional part and the reaction tube are sequentially connected together from top to bottom.
3. The integrated quantitative reaction tube according to claim 1 or 2, wherein the quantitative component comprises a pressing part and a pipette, the pressing part comprises a main body and a pressing air bag, the main body is a cup-shaped structure with an open upper end, the pressing air bag is installed at the opening of the main body in a sealing way or is integrally formed with the main body, the upper part of the pipette penetrates through the bottom of the main body and extends into a cavity formed by the cup-shaped structure of the main body and the pressing air bag, and the lower part of the pipette penetrates through the functional part and extends into the reaction tube.
4. A unitary dosing tube according to claim 3 wherein the bottom of the body is provided with the reservoir having a downward opening, and the functional portion is rotatable relative to the dosing assembly to effect the retention of a pre-set reagent in the reservoir or release into the tube in different angular positions.
5. The integrated quantitative reaction tube according to claim 4, wherein the functional part has an annular body, a functional partition is provided at a middle portion of the annular body, a plurality of through holes are provided on the functional partition, the functional partition physically seals the opening of the liquid storage chamber when the functional part is at some angular positions with respect to the quantitative component, and the through holes correspond to the opening of the liquid storage chamber when the functional part is at other angular positions, so that the reagent in the liquid storage chamber flows into the reaction tube through the through holes.
6. The integrated quantitative reaction tube according to claim 5, wherein the outer wall of the main body is provided with a guide buckle, the inner wall of the annular body and above the functional partition plate are provided with a screwing positioning groove, and the guide buckle is matched with the screwing positioning groove and can slide along the screwing positioning groove.
7. The integrated quantitative reaction tube according to claim 6, wherein the outer wall of the main body is positioned above the guide buckle, and the inner wall of the annular body is positioned above the functional partition plate and provided with mutually matched unidirectional teeth.
8. The integrated quantitative reaction tube according to claim 6, wherein the functional partition plate is provided with a pipette through hole for a pipette to pass through, and the pipette is provided with a pipette sealing ring which is in sealing fit with the pipette through hole.
9. The integrated quantitative reaction tube according to claim 6, wherein a liquid storage chamber sealing ring is arranged at the opening of the liquid storage chamber.
10. The integrated quantitative reaction tube according to claim 6, wherein the main body is provided with a first connection part connected with the functional part, the upper part of the functional part is provided with a second connection part adapted with the first connection part, the lower part of the functional part is provided with a third connection part connected with the reaction tube, and the upper part of the reaction tube is provided with a fourth connection part adapted with the third connection part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322928631.7U CN221192145U (en) | 2023-10-31 | 2023-10-31 | Integrated quantitative reaction tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322928631.7U CN221192145U (en) | 2023-10-31 | 2023-10-31 | Integrated quantitative reaction tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221192145U true CN221192145U (en) | 2024-06-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322928631.7U Active CN221192145U (en) | 2023-10-31 | 2023-10-31 | Integrated quantitative reaction tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221192145U (en) |
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2023
- 2023-10-31 CN CN202322928631.7U patent/CN221192145U/en active Active
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