CN111896512A - Lung cancer circulating tumor cell detection equipment - Google Patents
Lung cancer circulating tumor cell detection equipment Download PDFInfo
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- CN111896512A CN111896512A CN202010796298.7A CN202010796298A CN111896512A CN 111896512 A CN111896512 A CN 111896512A CN 202010796298 A CN202010796298 A CN 202010796298A CN 111896512 A CN111896512 A CN 111896512A
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- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 206010058467 Lung neoplasm malignant Diseases 0.000 title claims abstract description 27
- 201000005202 lung cancer Diseases 0.000 title claims abstract description 27
- 208000020816 lung neoplasm Diseases 0.000 title claims abstract description 27
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 title claims abstract description 23
- 210000004369 blood Anatomy 0.000 claims abstract description 61
- 239000008280 blood Substances 0.000 claims abstract description 61
- 239000003086 colorant Substances 0.000 claims abstract 3
- 210000004881 tumor cell Anatomy 0.000 claims description 30
- 239000011324 bead Substances 0.000 claims description 22
- 238000002372 labelling Methods 0.000 claims description 22
- 239000007850 fluorescent dye Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 claims description 4
- 230000010355 oscillation Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 206010027476 Metastases Diseases 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000003915 air pollution Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses lung cancer circulating tumor cell detection equipment which comprises a detection table, a storage bin and a centrifuge, wherein the storage bin is fixed above the detection table by adopting an upright column and comprises a blood sample bin and a fluorescent coloring agent bin, the blood sample bin can provide a blood sample for the centrifuge, and the centrifuge can carry out centrifugal treatment on the blood sample; centrifuge adopts the support arm to be fixed in one side of stand, centrifuge's discharge end is fixed to be communicated with drain pipe one, drain pipe one adopts the hose to provide the blood after handling for the mark subassembly, the mark subassembly can divide regional mark to the blood that enters into wherein and handle, and the different regional blood that just passes through after the mark is handled gets into respectively to count in the counting assembly that corresponds to the realization is to lung cancer circulating tumor cell's detection.
Description
Technical Field
The invention relates to the field of cell detection, in particular to lung cancer circulating tumor cell detection equipment.
Background
Smoking, air pollution or occupational hazards and the like can become causes of lung cancer, and the incidence rate and the death rate of the lung cancer are high, so that early diagnosis and recurrence monitoring of the lung cancer need to be paid particular attention to, the early diagnosis and recurrence monitoring of the lung cancer are particularly important for improving the 5-year survival rate of the lung cancer, and the detection of circulating tumor cells is beneficial to the diagnosis of a patient with tumor metastasis and the recurrence and metastasis of the tumor of the patient after operation.
However, in the present detection device, generally when utilizing blood centrifuge, magnetic bead reagent and fluorescence stain to carry out the mark detection, because the separation effect of magnetic bead complex is relatively poor, it shows that, even under the moderate condition of intensity in magnetic field, the magnetic bead of keeping away from magnetic field one side receives the influence less, and the separation effect is relatively poor, and the magnetic bead of being close to one side in magnetic field receives the influence great, though be favorable to the separation, nevertheless be unfavorable for the pump-out in later stage to later stage generally only adopts a set of equipment to detect, and its detection effect is relatively poor.
Therefore, it is necessary to provide a device for detecting circulating tumor cells of lung cancer, so as to solve the problems mentioned in the background art.
Disclosure of Invention
In order to achieve the purpose, the invention provides the following technical scheme: the lung cancer circulating tumor cell detection equipment comprises a detection table, a storage bin and a centrifuge, wherein the storage bin is fixed above the detection table by adopting a stand column and comprises a blood sample bin and a fluorescent staining agent bin, the blood sample bin can provide a blood sample for the centrifuge, and the centrifuge can centrifuge the blood sample;
centrifuge adopts the support arm to be fixed in one side of stand, centrifuge's the fixed intercommunication of discharge end has drain pipe one, drain pipe one adopts the hose to provide the blood after handling for the mark subassembly, the mark subassembly can divide regional mark to the blood that enters into wherein and handles, and the different regional blood that just passes through after the mark is handled gets into respectively to count in the counting assembly who corresponds.
Further, preferably, the marking assembly comprises a marking reaction bin, an oscillating assembly and a tumor cell enriching assembly, wherein the marking reaction bin can be used for quantitatively presetting magnetic bead reagents and receiving blood from a centrifuge, then, tumor cells in the blood in the marking reaction bin can be enriched through the tumor cell enriching assembly, so that two regions are divided according to the concentration of the tumor cells, and finally, different amounts of fluorescent staining agents are respectively added into the two regions and oscillated through the oscillating assembly to realize regional marking;
each area is communicated with a second liquid outlet pipe which is used for conveying blood to the counting assembly after the blood is marked in the areas.
Further, preferably, the tumor cell enrichment assembly comprises a mounting seat, a limiting guide seat, a sliding seat, a telescopic cylinder and a magnetic substance, wherein the mounting seat is groove-shaped, the limiting guide seat is symmetrically fixed in the mounting seat, a limiting guide groove is formed in the limiting guide seat, the sliding seat is slidably arranged in the limiting guide groove, and the sliding seat slidably extends out of the limiting guide seat to be fixedly connected with the marking reaction bin, so that the marking reaction bin can slide relative to the limiting guide seat;
the movement of the marking reaction bin is driven by a telescopic cylinder which is fixed on a limiting guide seat;
and a vertically arranged magnetic object is fixed on the other limiting guide seat.
Further, preferably, the telescopic action of the telescopic cylinder is controlled by an external air pump, and the extension and retraction actions of the telescopic cylinder are different, wherein when the telescopic cylinder extends to stop, the telescopic cylinder can push the labeling reaction chamber to simulate an emergency stop action so as to fully mix blood therein, so that all blood is in a process close to one side of the magnetic substance, and tumor cells are fully gathered on the left side of the labeling reaction chamber by a magnetic bead separation method; when the telescopic cylinder retracts to stop, the telescopic cylinder can slowly drive the marking reaction cabin to move, so that the blood in the telescopic cylinder is kept in a stable state, and the two regions can be divided according to the concentration of tumor cells.
Further, as preferred, telescopic cylinder adopts the mounting panel level to be fixed in the top of a spacing guide holder, just still be provided with buffer spring between mounting panel and the mark reaction bin.
Further, preferably, a plurality of groups of guide rollers are rotatably arranged in the mounting seat and between the two groups of limiting guide seats and are used for supporting and guiding the marking reaction bin.
Further, preferably, a first feeding hole and a second feeding hole are symmetrically communicated above the marking reaction bin, a first feeding pipe and a second feeding pipe are correspondingly and suspendedly arranged above the first feeding hole and the second feeding hole in the initial stage respectively, the first feeding pipe and the second feeding pipe are communicated with a fluorescent dye bin in the storage bin respectively through hoses, and the fluorescent dye bin can perform quantitative blanking on the first feeding pipe and the second feeding pipe respectively;
the first feeding pipe and the second feeding pipe are further arranged at the output end of the electric telescopic rod, and the electric telescopic rod is fixed below the storage bin by the aid of the mounting arm.
Further, as preferred, the vibration subassembly includes base, oscillator one, bears seat and guide bar, wherein, the base adopts the stand to be fixed in and examines on the test table, adopt multiunit oscillation spring to be connected with on the base and bear the seat, bear and be fixed with the mount pad on the seat, the below of bearing the seat is fixed with multiunit type of falling T guide bar, the guide bar slides and passes the base, it is first that still the symmetry is fixed with the oscillator on the base, the vertical linear oscillator of oscillator one, its output links to each other with bearing the seat.
Further, as preferred, the counting assembly includes a fluorescence electron microscope, a housing and an object observation assembly, wherein the lens ends of the object observation assembly and the fluorescence electron microscope are both disposed in the housing, and the fluorescence electron microscope can transmit the observation data thereof to the high-throughput cell image analyzer.
Further, as preferred, it includes objective table, second oscillator, buffer plate spring, year thing glass and sealed storehouse to carry the thing observation subassembly, wherein, second oscillator is fixed in on the objective table, second oscillator is horizontal linear oscillator, and its output adopts buffer plate spring to link to each other in year thing glass, be fixed with sealed storehouse on carrying the thing glass.
Compared with the prior art, the invention provides lung cancer circulating tumor cell detection equipment, which has the following beneficial effects:
1. the device is provided with a marking assembly, wherein the tumor cell enriching assembly can enrich the tumor cells in the blood in the marking reaction bin, and when the telescopic cylinder extends out to stop, the telescopic cylinder can push the marking reaction bin to simulate an emergency stop action so as to fully mix the blood in the marking reaction bin, so that all the blood has a process close to one side of a magnetic object, the tumor cells are fully gathered at the left side of the marking reaction bin by a magnetic bead separation method, and the magnetic bead combination is more comprehensively enriched; when the telescopic cylinder retracts to stop, the telescopic cylinder can slowly drive the labeling reaction bin to move to ensure that blood in the labeling reaction bin keeps a stable state, so that two areas can be divided according to the concentration of tumor cells, and after the magnetic bead combination is enriched on one side of the labeling reaction bin, the labeling reaction bin slowly moves back to an initial state position, at the moment, the influence of a magnetic field released by the magnetic substance on the magnetic beads is small, so that the incomplete pumping-out caused by the influence of the magnetic field is avoided when the magnetic bead mixture is pumped out in the follow-up process;
2. in this equipment, can realize the partition in two regions according to tumor cell's concentration, add the fluorescence staining agent of different weight respectively and oscillate through the oscillation subassembly in two regions, realize dividing regional mark, improved mark efficiency and mark degree of accuracy to can also be according to the region with the mixture pump sending wherein to the different counting assembly of two sets of specifications, so that divide regional detection, improve holistic detection accuracy.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a lung cancer circulating tumor cell detecting apparatus;
FIG. 2 is a schematic structural diagram of a marking assembly in a lung cancer circulating tumor cell detection device;
FIG. 3 is a schematic structural diagram of a carrier observation assembly in a lung cancer circulating tumor cell detection apparatus;
in the figure: 1. a detection table; 2. a storage bin; 3. a centrifuge; 4. a first liquid outlet pipe; 5. mounting an arm; 6. an electric telescopic rod; 7. a feeding pipe I; 8. a feeding pipe II; 9. a marking component; 10. a fluorescence electron microscope; 11. a housing; 12. a load observation assembly; 13. a base; 14. a first oscillator; 15. a bearing seat; 16. a guide bar; 17. a mounting seat; 18. a guide roller; 19. a limiting guide seat; 20. a limiting guide groove; 21. a sliding seat; 22. marking a reaction bin; 23. a telescopic cylinder; 24. a buffer spring; 25. a second liquid outlet pipe; 26. a first feeding hole; 27. a second feeding hole; 28. a magnetic substance; 29. an object stage; 30. a second oscillator; 31. a buffer plate spring; 32. carrying glass; 33. and (5) sealing the bin.
Detailed Description
Referring to fig. 1 to 3, in an embodiment of the present invention, a lung cancer circulating tumor cell detecting apparatus includes a detecting table 1, a storage chamber 2 and a centrifuge 3, wherein the storage chamber 2 is fixed above the detecting table 1 by using a column, the storage chamber 2 includes a blood sample chamber and a fluorescent staining agent chamber, the blood sample chamber can provide a blood sample for the centrifuge 3, and the centrifuge 3 can perform a centrifugal process on the blood sample, so as to separate red blood cells from the blood sample;
centrifuge 3 adopts the support arm to be fixed in one side of stand, centrifuge 3's the fixed intercommunication of discharge end has drain pipe 4, drain pipe 4 adopts the hose to provide the blood after handling for mark subassembly 9, and is concrete, is provided with the pump body on drain pipe 4's the hose to pump the liquid action, mark subassembly 9 can divide regional mark to entering into blood wherein and handle, and the different regional blood after the mark is handled gets into respectively to count in the count subassembly that corresponds.
In this embodiment, as shown in fig. 2, the labeling component 9 includes a labeling reaction chamber 22, an oscillating component, and a tumor cell enriching component, wherein the labeling reaction chamber 22 can be used for quantitatively presetting a magnetic bead reagent, and receiving blood from the centrifuge 3, and then tumor cells in the blood in the labeling reaction chamber 22 can be enriched by the tumor cell enriching component, so that two regions are divided according to the concentration of the tumor cells, and finally, different amounts of fluorescent dyes are respectively added to the two regions and oscillated by the oscillating component, so that the regional labeling is realized;
every region all communicates and is provided with drain pipe two 25 for carry blood to the counting assembly after regional mark of branch, specifically, drain pipe two 25 adopts elasticity bellows and counting assembly to be linked together, and is provided with the pump body on the elasticity bellows and carries out pump liquid.
In a preferred embodiment, the tumor cell enrichment assembly comprises a mounting seat 17, a limiting guide seat 19, a sliding seat 21, a telescopic cylinder 23 and a magnetic substance 28, wherein the mounting seat 17 is in a groove shape, the limiting guide seat 19 is symmetrically fixed in the mounting seat 17, a limiting guide groove 20 is formed in the limiting guide seat 19, the sliding seat 21 is slidably arranged in the limiting guide groove 20, the sliding seat 21 slidably extends out of the limiting guide seat 19 and is fixedly connected with a labeling reaction chamber 22, so that the labeling reaction chamber 22 can slide relative to the limiting guide seat 19;
the movement of the marking reaction bin 22 is driven by a telescopic cylinder 23, and the telescopic cylinder 23 is fixed on a limiting guide seat 19;
a magnetic substance 28 is fixed on the other of the position-limiting guide seats 19, and the magnetic substance releases a magnetic field, so that the magnetic bead conjugate moves to the position of the magnetic substance 28 and is enriched.
In order to make the enrichment of magnetic beads more comprehensive, the telescopic action of the telescopic cylinder 23 is controlled by an external air pump, and the extension and retraction actions of the telescopic cylinder 23 are different, wherein when the telescopic cylinder 23 extends to a stop, it can push the labeling reaction chamber 22 to simulate an emergency stop action, so as to fully mix the blood therein, so that all the blood has a process of being located at one side close to the magnetic substance 28, so as to fully gather tumor cells at the left side of the labeling reaction chamber 22 by using a magnetic bead separation method; when the telescopic cylinder 23 retracts to stop, the telescopic cylinder can slowly drive the labeling reaction bin 22 to move, so that the blood in the labeling reaction bin can be kept in a stable state, the two regions can be divided according to the concentration of tumor cells, in addition, it is worth mentioning that after the magnetic bead combination is enriched on one side of the labeling reaction bin 22, the labeling reaction bin 22 slowly moves back to the initial state position, the influence of the magnetic field released by the magnetic bead combination on the magnetic bead is small, and the incomplete pumping caused by the influence of the magnetic field is avoided when the magnetic bead mixture is pumped out subsequently.
In this embodiment, the telescopic cylinder 23 is horizontally fixed above a limit guide seat 19 by using a mounting plate, and a buffer spring 24 is further arranged between the mounting plate and the mark reaction bin 22.
In a preferred embodiment, a plurality of sets of guide rollers 18 are rotatably disposed in the mounting seat 17 and between two sets of limiting guide seats 19, and are used for supporting and guiding the marking reaction chamber 22, so that the movement of the marking reaction chamber 22 is more stable.
In this embodiment, as shown in fig. 1 and 2, a first feeding hole 26 and a second feeding hole 27 are symmetrically arranged above the marking reaction bin 22 in a communicating manner, and in an initial stage, a first feeding pipe 7 and a second feeding pipe 8 are respectively arranged above the first feeding hole 26 and the second feeding hole 27 in a corresponding and suspending manner, the first feeding pipe 7 and the second feeding pipe 8 are respectively communicated with the fluorescent dye bin in the storage bin 2 by using a hose, the fluorescent dye bin can respectively perform quantitative blanking for the first feeding pipe 7 and the second feeding pipe 8, specifically, two blanking electromagnetic valves are arranged in the fluorescent dye bin, a flow meter is arranged at a blanking port of each blanking electromagnetic valve, the blanking amount is monitored by the flow meter, so that quantitative blanking can be realized, wherein a material body inserted into the first feeding pipe 7 is more than a material body inserted into the second feeding pipe 8, so as to perform blanking marking according to concentrations of tumor cells with different local cycles, the marking efficiency and the marking accuracy are improved;
the feeding pipe I7 and the feeding pipe II 8 are further arranged at the output end of the electric telescopic rod 6, and the electric telescopic rod 6 is fixed below the storage bin 2 through the mounting arm 5.
As a preferred embodiment, the oscillating assembly includes a base 13, a first oscillator 14, a bearing seat 15 and a guide rod 16, wherein the base 13 is fixed on the detection table 1 by using a vertical column, the base 13 is connected with the bearing seat 15 by using a plurality of sets of oscillating springs, a mounting seat 17 is fixed on the bearing seat 15, a plurality of sets of inverted T-shaped guide rods 16 are fixed below the bearing seat 15, the guide rods 16 slide through the base 13, the base 13 is further symmetrically fixed with the first oscillator 14, an output end of the vertical linear oscillator of the first oscillator 14 is connected with the bearing seat 15, and the vertical linear oscillating action of the first oscillator 14 can realize respective oscillation in different areas and reduce the mutual communication influence thereof.
In this embodiment, as shown in fig. 1 and fig. 3, the counting assembly includes a fluorescence electron microscope 10, a housing 11 and an object observing assembly, wherein the lens ends of the object observing assembly 12 and the fluorescence electron microscope 10 are disposed in the housing 11, the fluorescence electron microscope 10 can transmit the observation data thereof to the high-throughput cellular image analyzer, it should be noted that the labeling reaction chamber 22 can pump the mixture therein to two sets of counting assemblies according to regions, and the two sets of counting assemblies have different specifications, which are expressed by the difference of the observation accuracy of the fluorescence electron microscope 10, the oscillation intensity of the oscillator two in the object observing assembly 12, and the difference of the sizes of the object stages, so as to perform the regional detection and improve the overall detection accuracy.
In a preferred embodiment, the object observing assembly 12 includes an object stage 29, a second oscillator 30, a buffer plate spring 31, an object glass 32 and a sealed chamber 33, wherein the second oscillator 30 is fixed on the object stage 29, the second oscillator 30 is a horizontal linear oscillator, an output end of the second oscillator is connected to the object glass 32 by the buffer plate spring 31, the sealed chamber 33 is fixed on the object glass 32, wherein the second drain pipe 25 is communicated with the object observing assembly 12 through an elastic corrugated pipe, and specifically, the elastic corrugated pipe passes through the housing 11 and is communicated with one side of the sealed chamber 33.
In specific implementation, a blood sample is provided for the centrifuge 3 by using a blood sample bin, the centrifuge 3 can perform centrifugal processing on the blood sample, so as to separate red blood cells in the blood sample, then processed blood is provided for the marking component 9 through the first liquid outlet pipe 4 at the discharge end of the centrifuge 3, a magnetic bead reagent can be quantitatively preset in the marking reaction bin 22 in the marking component 9, and the blood from the centrifuge 3 is received, then tumor cells in the blood in the marking reaction bin 22 are enriched through the tumor cell enrichment component, so as to realize division of two regions according to the concentration of the tumor cells, finally, fluorescent dyes with different components are respectively added into the two regions and are oscillated through the oscillating component, so as to realize division and marking, each region is communicated with the second liquid outlet pipe 25, so as to convey the blood into the counting component after division and marking, the fluorescence electron microscope 10 can transmit the observation data to a high-throughput cell image analyzer to realize detection.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.
Claims (10)
1. The lung cancer circulating tumor cell detection equipment comprises a detection table (1), a storage bin (2) and a centrifuge (3), wherein the storage bin (2) is fixed above the detection table (1) by adopting a stand column, the storage bin (2) comprises a blood sample bin and a fluorescent stain bin, the blood sample bin can provide a blood sample for the centrifuge (3), and the centrifuge (3) can centrifuge the blood sample;
the method is characterized in that: centrifuge (3) adopt the support arm to be fixed in one side of stand, centrifuge (3)'s the fixed intercommunication of discharge end has drain pipe (4), drain pipe (4) adopt the hose to provide the blood after handling for mark subassembly (9), mark subassembly (9) can divide regional mark to the blood that gets into wherein and handle, and the different regional blood that processes after the mark gets into respectively to count in the counting assembly that corresponds.
2. The apparatus for detecting circulating tumor cells of lung cancer according to claim 1, wherein: the marking assembly (9) comprises a marking reaction bin (22), an oscillating assembly and a tumor cell enriching assembly, wherein the marking reaction bin (22) can be used for quantitatively presetting a magnetic bead reagent and receiving blood from the centrifuge (3), then, tumor cells in the blood in the marking reaction bin (22) can be enriched through the tumor cell enriching assembly, so that two regions are divided according to the concentration of the tumor cells, and finally, different amounts of fluorescent staining agents are respectively added into the two regions and are oscillated through the oscillating assembly to realize regional marking;
each area is communicated with a second liquid outlet pipe (25) for conveying blood to the counting assembly after the area is marked in a divided mode.
3. The apparatus for detecting circulating tumor cells of lung cancer according to claim 1, wherein: the tumor cell enrichment assembly comprises a mounting seat (17), a limiting guide seat (19), a sliding seat (21), a telescopic cylinder (23) and a magnetic object (28), wherein the mounting seat (17) is groove-shaped, the limiting guide seat (19) is symmetrically fixed in the mounting seat (17), a limiting guide groove (20) is formed in the limiting guide seat (19), the sliding seat (21) is arranged in the limiting guide groove (20) in a sliding manner, the sliding seat (21) extends out of the limiting guide seat (19) in a sliding manner and is fixedly connected with a marking reaction bin (22), and therefore the marking reaction bin (22) can slide relative to the limiting guide seat (19);
the movement of the marking reaction bin (22) is driven by a telescopic cylinder (23), and the telescopic cylinder (23) is fixed on a limiting guide seat (19);
a magnetic object (28) which is vertically arranged is fixed on the other limit guide seat (19).
4. The apparatus for detecting circulating tumor cells of lung cancer according to claim 3, wherein: the telescopic action of the telescopic cylinder (23) is controlled by an external air pump, and the extension and retraction actions of the telescopic cylinder (23) are different, wherein when the telescopic cylinder (23) extends to stop, the telescopic cylinder can push the labeling reaction chamber (22) to simulate an emergency stop action so as to fully mix blood therein, so that all blood is in a process of being close to one side of the magnetic substance (28), and tumor cells are fully gathered on the left side of the labeling reaction chamber (22) by a magnetic bead separation method; when the telescopic cylinder (23) retracts to stop, the telescopic cylinder can slowly drive the marking reaction bin (22) to move, so that the blood in the telescopic cylinder is ensured to be kept in a stable state, and the two regions can be divided according to the concentration of tumor cells.
5. The apparatus for detecting circulating tumor cells of lung cancer according to claim 3, wherein: the telescopic cylinder (23) is horizontally fixed above a limiting guide seat (19) by adopting a mounting plate, and a buffer spring (24) is also arranged between the mounting plate and the mark reaction bin (22).
6. The apparatus for detecting circulating tumor cells of lung cancer according to claim 3, wherein: and a plurality of groups of guide rollers (18) are rotatably arranged in the mounting seat (17) and between the two groups of limiting guide seats (19) and are used for supporting and guiding the marking reaction bin (22).
7. The apparatus for detecting circulating tumor cells of lung cancer according to claim 2, wherein: a first feeding hole (26) and a second feeding hole (27) are symmetrically communicated above the marking reaction bin (22), in an initial stage, a first feeding pipe (7) and a second feeding pipe (8) are respectively arranged above the first feeding hole (26) and the second feeding hole (27) in a corresponding and hanging mode, the first feeding pipe (7) and the second feeding pipe (8) are respectively communicated with a fluorescent coloring agent bin in the storage bin (2) through hoses, and the fluorescent coloring agent bins can respectively carry out quantitative blanking on the first feeding pipe (7) and the second feeding pipe (8);
the feeding pipe I (7) and the feeding pipe II (8) are further arranged at the output end of the electric telescopic rod (6), and the electric telescopic rod (6) is fixed below the storage bin (2) through the mounting arm (5).
8. The apparatus for detecting circulating tumor cells of lung cancer according to claim 3, wherein: the oscillating assembly comprises a base (13), a first oscillator (14), a bearing seat (15) and a guide rod (16), wherein the base (13) is fixed on the detection platform (1) by adopting a stand column, the base (13) is connected with the bearing seat (15) by adopting a plurality of groups of oscillating springs, a mounting seat (17) is fixed on the bearing seat (15), a plurality of groups of inverted T-shaped guide rods (16) are fixed below the bearing seat (15), the guide rod (16) slides to pass through the base (13), the base (13) is symmetrically fixed with the first oscillator (14), and the vertical linear oscillator of the first oscillator (14) is connected with the bearing seat (15) at the output end.
9. The apparatus for detecting circulating tumor cells of lung cancer according to claim 1, wherein: the counting assembly comprises a fluorescence electron microscope (10), a housing (11) and an object observation assembly, wherein the lens ends of the object observation assembly and the fluorescence electron microscope (10) are arranged in the housing (11), and the fluorescence electron microscope (10) can transmit observation data of the fluorescence electron microscope into the high-flux cell image analyzer.
10. The apparatus for detecting circulating tumor cells of lung cancer according to claim 9, wherein: the object carrying observation assembly comprises an object carrying table (29), a second oscillator (30), a buffer plate spring (31), object carrying glass (32) and a sealed bin (33), wherein the second oscillator (30) is fixed on the object carrying table (29), the second oscillator (30) is a horizontal linear oscillator, the output end of the second oscillator is connected with the object carrying glass (32) through the buffer plate spring (31), and the sealed bin (33) is fixed on the object carrying glass (32).
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| CN202010796298.7A CN111896512B (en) | 2020-08-10 | 2020-08-10 | Lung cancer circulating tumor cell detection equipment |
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| CN202010796298.7A CN111896512B (en) | 2020-08-10 | 2020-08-10 | Lung cancer circulating tumor cell detection equipment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117571437A (en) * | 2023-11-20 | 2024-02-20 | 广州毅昌科技股份有限公司 | Quantitative sample cup-in extraction device |
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