CN117502452A - Synergistic bactericidal composition for preventing and treating jackfruit rot - Google Patents

Synergistic bactericidal composition for preventing and treating jackfruit rot Download PDF

Info

Publication number
CN117502452A
CN117502452A CN202311637299.7A CN202311637299A CN117502452A CN 117502452 A CN117502452 A CN 117502452A CN 202311637299 A CN202311637299 A CN 202311637299A CN 117502452 A CN117502452 A CN 117502452A
Authority
CN
China
Prior art keywords
jackfruit
rot
farnesol
treating
preventing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202311637299.7A
Other languages
Chinese (zh)
Inventor
宁琳
何江
朱鹏锦
欧景莉
陈燕
朱杨帆
唐秀观
叶维雁
陈豪军
杜英俊
钟云婕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Subtropical Crops Research Institute
Original Assignee
Guangxi Subtropical Crops Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Subtropical Crops Research Institute filed Critical Guangxi Subtropical Crops Research Institute
Priority to CN202311637299.7A priority Critical patent/CN117502452A/en
Publication of CN117502452A publication Critical patent/CN117502452A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N49/00Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/561,2-Diazoles; Hydrogenated 1,2-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647Triazoles; Hydrogenated triazoles
    • A01N43/6531,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of pesticides, in particular to a synergistic bactericidal composition for preventing and treating jackfruit rot. The synergistic bactericidal composition for preventing and treating jackfruit rot is prepared by compounding active ingredients of farnesol and benzovindiflupyr; the mass ratio of the farnesol to the benzovindiflupyr is 1-10:25-1. On the basis of the existing pesticides, the invention combines the effective components of different pesticides, screens out synergistic combinations, can improve the control effect of jackfruit rot, is beneficial to reducing the pesticide application dosage and delaying the generation of pathogenic bacteria drug resistance.

Description

Synergistic bactericidal composition for preventing and treating jackfruit rot
Technical Field
The invention relates to the technical field of pesticides, in particular to a synergistic bactericidal composition for preventing and treating jackfruit rot.
Background
Both aschersonia (Cylindrocladium sp.) and curvatus (l. Thenobromae) and the like can cause jackfruit rot. Among them, the cocoa hair color two spore (l. Theobromae) can be parasitic to more than 500 plants in tropical and subtropical areas, causing various diseases of field crops, vegetables, fruits and woods, mainly including various types of shoot blight, branch blight, root rot, fruit rot, leaf spot, ulcer and gummosis.
The jackfruit cocoa hairy color two-spore fruit rot mainly damages jackfruit male flowers and fruits. When the male flowers are infected, the initially infected parts turn brown and spread to four weeks rapidly, and the whole male flowers are infected by pathogenic bacteria rapidly; the male flowers become black and fall off in the later stage of the disease. Any part of the fruit can be diseased, the disease spots are irregular, the initial infected pericarp turns brown, and the surface is accompanied with white hyphae; the initial part of the jackfruit at the later stage of the disease turns black brown, which causes the rot of the fruit and affects the yield and quality of jackfruit.
At present, chemical agents are one of the more remarkable means for preventing and treating jackfruit rot, but the resistance of pathogenic bacteria of the jackfruit rot to various agents used at present is different degrees due to the unscientific medication modes such as long-term singleness, continuity, repeatability and the like. The unscientific medication mode shortens the generation time of resistance of various medicaments year by year, and greatly increases the prevention and treatment cost, the environmental cost and the medicament research and development cost. Based on the current situation, improving the prevention and treatment effect of the medicine based on the prior art is an economical and effective means for solving the problems.
The compounding of the active ingredients of different pesticides is an effective and rapid way of developing and developing new pesticides and preventing and treating agricultural resistant bacteria at present. After the active ingredients of different pesticides are compounded, three action types are generally shown: additive action, synergistic action and antagonistic action. The compound formulation with good synergy can obviously improve the actual control effect and reduce the use amount of pesticides, thereby greatly delaying the generation speed of pathogen resistance, and being an important means for comprehensively controlling diseases.
The inventor discovers that the compound of the farnesol and the sedaxane, the benzovindiflupyr or the cyproconazole in a certain mass ratio range shows a synergistic effect on jackfruit rot, and no report of related compound is found at present.
Disclosure of Invention
The invention aims to provide a synergistic bactericidal composition for preventing and treating jackfruit rot, which is prepared by compounding different pesticide active ingredients on the basis of the existing pesticide, and screening out synergistic combinations, so that the preventing and treating effect of jackfruit rot can be improved, the pesticide application dosage can be reduced, and the generation of pathogenic bacteria drug resistance can be delayed.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the synergistic bactericidal composition for preventing and treating jackfruit rot is prepared by compounding farnesol, cyproconazole, benzovindiflupyr or cyproconazole as effective components.
Preferably, the mass ratio of the farnesol to the cyproconazole is 1-40:2-1.
Preferably, the mass ratio of the farnesol to the benzovindiflupyr is 1-10:25-1.
Preferably, the mass ratio of the farnesol to the cyproconazole is 1-9:5-1.
The invention also aims to provide the application of the bactericidal composition in preventing and treating jackfruit rot.
Compared with the prior art, the invention has the following beneficial effects:
the composition has good synergistic effect after the active ingredient of the famous alcohol and the cyproconazole, the benzovindiflupyr or the cyproconazole are compounded, can improve the control effect of jackfruit rot, is beneficial to reducing the application dosage of pesticides and delaying the generation of drug resistance of pathogenic bacteria.
Detailed Description
The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.
Examples: indoor biological activity test of compound farnesol on jackfruit rot
Test object: and selecting diseased fruits in a jackfruit plantation, purifying the diseased fruits by a laboratory separation method to obtain the cocoa hair color bisporus (Lasiodiplodia theobromae), and storing the cocoa hair color bisporus on a PDA (personal digital assistant) culture medium.
Test agent: 98% of farnesol technical (Shanghai Yuan leaf Biotechnology Co., ltd.), 96% of penflufen technical (Lithospermum candidum) 96% of benzovindiflupyr technical (Lithospermum candidum) and 95% of cyproconazole technical (Lithospermum candidum)
The test method comprises the following steps: ( Reference to section 2 of the pesticide "NY/T1156.2-2006 indoor bioassay test guidelines for pesticides: test Petri dish method for inhibiting growth of pathogenic fungi hyphae )
1. Dissolving the raw materials with dimethyl sulfoxide, diluting with 0.1% Tween-80 water solution to obtain single-dose mother solution, and setting multiple groups of proportions, wherein each single dose and each group of proportion are provided with 5 gradient mass concentrations.
2. 9mL of the PDA culture medium which is melted in advance is added into a sterile conical flask, 1mL of the liquid medicine is quantitatively sucked from low concentration and high concentration in sequence, and the liquid medicine is respectively added into the conical flask and fully and uniformly shaken. Then pouring the same amount into 3 culture dishes with the diameter of 9cm to prepare a medicine-containing flat plate with corresponding concentration; treatments without agent were included as blank, 3 replicates per treatment.
3. And (3) taking bacterial cakes with the diameter of 5mm from the bacterial colonies of the colletotrichum gloeosporioides after propagation by using a puncher, inoculating the centers of a medicine-containing flat plate and a blank control flat plate, and culturing in a constant-temperature incubator at 28 ℃.
After 4.24 hours, colony diameters were measured by the crisscross method, and the hypha growth inhibition rates of different treatments were calculated. Taking the medicine logarithmic value as an independent variable and the hypha growth inhibition rate value as a dependent variable, analyzing by using DPS software to obtain a virulence regression equation and the virulence EC of the medicine to target bacteria 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
D=D 1 -D 2 Wherein D is colony growth diameter; d (D) 1 Is colony diameter; d (D) 2 The diameter of the fungus cake;
I=[(D 0 -D t )÷D0]x 100, wherein I is the hypha growth inhibition (%); d (D) 0 Growing the diameter of a blank control colony; d (D) t Colony growth diameter for agent treatment;
measured virulence index (ATI) = (standard agent EC 50 Reagent EC 50 )×100;
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture;
co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/(theoretical drug susceptibility index (TTI) ] x 100.
Dividing the standard according to the joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, and the synergistic effect is shown; the cotoxicity coefficient (CTC) is less than or equal to 80 and shows antagonism; 80< co-toxicity coefficient (CTC) <120 shows additive effect. The experimental results are shown in tables 1-3.
TABLE 1 determination of indoor biological Activity of famous alcohol and Fluozolyl Cycloxafen in combination with jackfruit rot pathogen
Medicament name and ratio EC50(mg/L) ATI TTI CTC
Farnesol 18.3491 100.0000 -- --
Cyproconazole 1.7283 1061.6849 -- --
Farnesol 1: cyproconazole 2 1.8704 981.0254 741.1233 132.3701
Farnesol 1: cyproconazole 1 2.5511 719.2623 580.8424 123.8309
Farnesol 3: cyproconazole 1 3.7088 494.7449 340.4212 145.3332
Farnesol 7: cyproconazole 1 6.0529 303.1456 220.2106 137.6617
Farnesol 15: cyproconazole 1 4.4881 408.8389 160.1053 255.3563
Farnesol 30: cyproconazole 1 9.8974 185.3931 131.0221 141.4976
Farnesol 40: cyproconazole 1 11.5630 158.6881 123.4557 128.5384
As can be seen from Table 1, the mass ratio is 1-40:2-1, the co-toxicity coefficient of the compound of the farnesol and the sedaxane to the jackfruit rot pathogen is 123.8309-255.3563, which is greater than 120, and the synergistic effect is shown.
Table 2 determination of indoor biological Activity of farnesol and Benzenofloxacin Complex against jackfruit rot pathogen
Medicament name and ratio EC50(mg/L) ATI TTI CTC
Farnesol 18.3491 100.0000 -- --
Benzovindiflupyr 10.5042 174.6835 -- --
Farnesol 1: benzovindiflupyr 25 8.8170 208.1105 171.8110 121.1275
Farnesol 1: benzovindiflupyr 15 5.0460 363.6365 170.0157 213.8840
Farnesol 1: benzovindiflupyr 7 7.1186 257.7628 165.3480 155.8910
Farnesol 1: benzovindiflupyr 3 2.8687 639.6312 156.0126 409.9869
Farnesol 1: benzovindiflupyr 1 4.0190 456.5588 137.3417 332.4254
Farnesol 3: benzovindiflupyr 1 3.4194 536.6175 118.6709 452.1898
Farnesol 7: benzovindiflupyr 1 1.4122 1299.3273 109.3354 1188.3863
Farnesol 10: benzovindiflupyr 1 10.0151 183.2143 106.7894 171.5660
As can be seen from table 2, the mass ratio is 1-10: within 25-1, the co-toxicity coefficients of the compound of the farnesol and the benzovindiflupyr to the jackfruit rot pathogen are between 121.1275 and 1188.3863 and are all more than 120, and the synergistic effect is shown; in particular, the mass ratio is 7: in the 1, the co-toxicity coefficient reaches 1188.3863, and the synergistic effect is most remarkable.
TABLE 3 determination of indoor biological Activity of Fanniol and cyproconazole in combination with jackfruit rot pathogen
Medicament name and ratio EC50(mg/L) ATI TTI CTC
Farnesol 18.3491 100.0000 -- --
Cyproconazole 3.1429 583.8270 -- --
Farnesol 1: cyproconazole 5 2.6527 691.7141 503.1892 137.4660
Farnesol 1: cyproconazole 3 3.1786 577.2699 462.8703 124.7153
Farnesol 1: cyproconazole 1 4.0142 457.1048 341.9135 133.6902
Farnesol 3: cyproconazole 1 5.0338 364.5179 220.9568 164.9725
FaniAlcohol 5: cyproconazole 1 7.7554 236.5977 180.6378 130.9790
Farnesol 9: cyproconazole 1 10.0853 181.9391 148.3827 122.6147
As can be seen from table 3, the mass ratio is 1-9:5-1, the co-toxicity coefficient of the compound of the farnesol and the cyproconazole on the jackfruit rot pathogen is 122.6147-164.9725, and the co-toxicity coefficient is more than 120, and the synergistic effect is shown.
In conclusion, the active ingredient of the composition disclosed by the invention has a good synergistic effect after being compounded with the pennisetum and the sedaxane, the benzovindiflupyr or the cyproconazole, can improve the control effect of jackfruit rot, is beneficial to reducing the application dosage of pesticides and delaying the generation of drug resistance of pathogenic bacteria.
The foregoing is merely illustrative of the preferred embodiments of this invention and modifications, obvious to those skilled in the art, may be made without departing from the principles of this invention and are within the scope of this invention.

Claims (2)

1. The synergistic bactericidal composition for preventing and treating jackfruit rot is characterized in that the effective components are compounded by farnesol and benzovindiflupyr; the mass ratio of the farnesol to the benzovindiflupyr is 1-10:25-1.
2. The use of the synergistic bactericidal composition of claim 1 for controlling jackfruit rot.
CN202311637299.7A 2022-09-09 2022-09-09 Synergistic bactericidal composition for preventing and treating jackfruit rot Pending CN117502452A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311637299.7A CN117502452A (en) 2022-09-09 2022-09-09 Synergistic bactericidal composition for preventing and treating jackfruit rot

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202311637299.7A CN117502452A (en) 2022-09-09 2022-09-09 Synergistic bactericidal composition for preventing and treating jackfruit rot
CN202211101474.6A CN115413671B (en) 2022-09-09 2022-09-09 Sterilization composition for preventing and treating jackfruit rot

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN202211101474.6A Division CN115413671B (en) 2022-09-09 2022-09-09 Sterilization composition for preventing and treating jackfruit rot

Publications (1)

Publication Number Publication Date
CN117502452A true CN117502452A (en) 2024-02-06

Family

ID=84201960

Family Applications (3)

Application Number Title Priority Date Filing Date
CN202311637300.6A Pending CN117502453A (en) 2022-09-09 2022-09-09 Sterilization composition for improving control effect of jackfruit rot
CN202211101474.6A Active CN115413671B (en) 2022-09-09 2022-09-09 Sterilization composition for preventing and treating jackfruit rot
CN202311637299.7A Pending CN117502452A (en) 2022-09-09 2022-09-09 Synergistic bactericidal composition for preventing and treating jackfruit rot

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CN202311637300.6A Pending CN117502453A (en) 2022-09-09 2022-09-09 Sterilization composition for improving control effect of jackfruit rot
CN202211101474.6A Active CN115413671B (en) 2022-09-09 2022-09-09 Sterilization composition for preventing and treating jackfruit rot

Country Status (1)

Country Link
CN (3) CN117502453A (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201912595D0 (en) * 2019-09-02 2019-10-16 Syngenta Crop Protection Ag Plant growth regulator compounds
CN113615693B (en) * 2019-11-05 2022-11-29 山东康惠植物保护有限公司 Anti-anthracnose respiratory inhibitor

Also Published As

Publication number Publication date
CN115413671A (en) 2022-12-02
CN115413671B (en) 2023-12-22
CN117502453A (en) 2024-02-06

Similar Documents

Publication Publication Date Title
CN114794130B (en) Agent for preventing and treating anthracnose of macadimia nut
CN115413671B (en) Sterilization composition for preventing and treating jackfruit rot
CN112438259A (en) Bactericide composition for preventing and treating white disease of foxtail millet
CN115530176B (en) Bactericide for preventing and treating anthracnose of avocado
CN114847297B (en) Pesticide composition for preventing and treating rice diseases
CN113994972B (en) Composition for treating gummosis of nectarines
CN113558059B (en) Composition containing zhongshengmycin and natamycin
CN114391551B (en) Novel fertilizer special for strawberries
CN115024332B (en) Pesticide composition for preventing and treating pepper anthracnose
CN115918665B (en) Wheat seed dressing agent for preventing and treating wheat stem-based rot
CN114946869B (en) Macadimia nut recession disease control agent for disease resistance breeding
CN111937895B (en) Application of pyraclostrobin and composition containing pyraclostrobin in prevention and treatment of peach branch blight
CN117044733A (en) Biopesticide for preventing and treating anthracnose of avocado
CN116649348A (en) Pesticide composition for preventing and treating damping-off of white gourd
CN115053900A (en) Bactericide for preventing and treating avocado canker
CN116649340A (en) Bactericide composition for preventing and treating damping-off of white gourd
CN116784345A (en) Composition for improving nitrogen absorption of wheat plants
CN117099780A (en) Bactericidal composition containing aloe-emodin
CN114304168A (en) Bactericidal composition containing benziothiazolinone and isoprothiolane and application thereof
CN116210700A (en) Sterilization composition for preventing and treating strawberry anthracnose
CN116649375A (en) Green planting method of organic rice
CN117044723A (en) Synergistic composition for preventing and treating mango anthracnose
CN116602308A (en) Bactericide composition for preventing and treating bacterial wilt of momordica grosvenori
CN117099781A (en) Synergistic composition containing aloe-emodin
CN117530282A (en) Prevention and treatment agent for fruit black spot

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination