CN117461530A - Root tuber-based large-scale breeding method for radix asparagi in river - Google Patents
Root tuber-based large-scale breeding method for radix asparagi in river Download PDFInfo
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- CN117461530A CN117461530A CN202311701065.4A CN202311701065A CN117461530A CN 117461530 A CN117461530 A CN 117461530A CN 202311701065 A CN202311701065 A CN 202311701065A CN 117461530 A CN117461530 A CN 117461530A
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- root
- culture medium
- radix asparagi
- river
- breeding method
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- 238000009395 breeding Methods 0.000 title claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 17
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 10
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000006013 carbendazim Substances 0.000 claims abstract description 10
- 239000002689 soil Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000003892 spreading Methods 0.000 claims abstract description 4
- 238000009423 ventilation Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 238000005406 washing Methods 0.000 claims abstract description 3
- 239000003415 peat Substances 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 235000013311 vegetables Nutrition 0.000 claims description 3
- 239000010455 vermiculite Substances 0.000 claims description 3
- 235000019354 vermiculite Nutrition 0.000 claims description 3
- 229910052902 vermiculite Inorganic materials 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 10
- 241000234427 Asparagus Species 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 8
- 235000005340 Asparagus officinalis Nutrition 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 244000248539 Asparagus cochinchinensis Species 0.000 description 3
- 235000009292 Asparagus cochinchinensis Nutrition 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000432824 Asparagus densiflorus Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010013789 Dry throat Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 206010039424 Salivary hypersecretion Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 208000026451 salivation Diseases 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a large-scale breeding method of radix asparagi in Neijiang based on tuberous root, which selects the inner Jiang Tiandong with cultivation age and strong growth over 2 years as a parent plant, and selects the tuberous root with complete root at two ends of the tuberous root without obvious damage and proper size; soaking the tuberous root in carbendazim solution, washing with water, and drying in shade at ventilation place; preparing a seedbed by selecting well-drained soil, and paving a culture medium on a common Miao Chuangshang with the thickness of 10-15cm; spreading the tuberous root on a culture medium according to row arrangement, covering the culture medium by about 5cm, finally watering, erecting a sunshade net, wherein the sunshade rate is 50-75%, and the temperature is 25-30 ℃. The method is simple and efficient, has a larger propagation coefficient compared with the separation of plants, and can be used for large-scale propagation of the radix asparagi in the river.
Description
Technical Field
The invention relates to the field of seedling breeding, in particular to a large-scale breeding method of radix asparagi in river based on tuberous root.
Background
Asparagus is a common traditional Chinese medicine of Asparagus plant of Liliaceae, and has sweet and slightly bitter taste and cold nature, and enters lung and kidney meridians. Has effects of nourishing yin, moistening dryness, clearing lung-heat and promoting salivation. Can be used for treating cough due to lung dryness, cough, excessive phlegm, soreness of waist and knees, hectic fever due to bone steaming, internal heat, thirst, and constipation due to dry throat and dry intestine. The interior Jiang Tiandong is a Sichuan radix rehmanniae medicine, has a sweet taste compared with other radix asparagi in the producing area, and is recorded in the 'county of Neijiang', and farmers plant radix asparagi as early as about 19 years (1840) of Qingxianfeng.
Currently, asparagus is an important material base and valuable resource for sustainable development of the large health industry. The asparagus fern is hardly seeded due to long-term artificial cultivation, and is usually obtained in a plant division mode for propagation. The plant propagation has the defects of difficult sterilization of materials, high seedling cost, large transportation difficulty and the like. In particular, the inner Jiang Tiandong has the defect of low propagation coefficient in a plant dividing mode, one parent plant can be divided into more than 10 plant seedlings, and the current seedling requirement and supply shortage state are far difficult to meet.
Therefore, a set of large-scale breeding methods with high breeding coefficients and low cost are urgently needed to be established, and the large-scale breeding method of seedlings is a precondition for artificial planting and is also a requirement for development of the large and healthy asparagus industry.
Disclosure of Invention
Therefore, one of the purposes of the invention is to provide a root tuber-based large-scale breeding method for radix asparagi in Neijiang.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the large-scale breeding method of the radix asparagi in the river based on the tuberous root comprises the following steps:
(1) Selecting an inner Jiang Tiandong with a cultivation period of more than 2 years and strong growth as a mother plant, and selecting root tubers with complete roots at two ends of the root tubers, no obvious damage and proper size;
(2) Soaking the tuberous root in carbendazim solution, washing with water, and drying in shade at ventilation place;
(3) Preparing a seedbed by selecting well-drained soil, and paving a culture medium on a common Miao Chuangshang with the thickness of 10-15cm;
(4) Spreading the tuberous root on a culture medium according to row arrangement, covering the culture medium by about 5cm, finally watering, erecting a sunshade net, wherein the sunshade rate is 50-75%, and the temperature is 25-30 ℃.
Preferably, the culture medium is prepared from vegetable garden soil: peat soil: vermiculite: the mass ratio of the perlite is 3:3:3:0.5.
Preferably, the root tuber size is 18-19g.
In the invention, the carbendazim is soaked in a carbendazim solution with the mass percentage of 0.1% for 10 minutes.
The invention has the beneficial effects that:
the invention discloses a large-scale breeding method of inner Jiang Tiandong seedlings, which is established by establishing a asexual propagation system based on tubers. Firstly, cutting off tuberous roots from a mother plant, sterilizing and airing surface moisture; the sun-shading net is erected to control illumination, and inner Jiang Tiandong seedlings are obtained by guaranteeing moisture and then emergence of seedlings. The seedlings can be obtained from the tubers after about 180 days, can be used for large-scale seed production after rapid propagation of single genetic background materials of excellent asparagus resources, is suitable for large-scale culture, and provides assistance for the development of the asparagus industry in the interior river.
Drawings
In order to make the objects, technical solutions and advantageous effects of the present invention more clear, the present invention provides the following drawings for description:
FIG. 1 shows root tuber emergence characteristics (A: different parts of Asparagus cochinchinensis, B: details of the inner Jiang Tiandong roots and shoots);
FIG. 2 shows the breeding cycle of Asparagus cochinchinensis (A: inner Jiang Tiandong stock; B: inner Jiang Kuaigen; C: inner Jiang Tiandong seedling formation condition (90D; D: inner Jiang Tiandong seedling formation effect (180D)).
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific examples, which are not intended to limit the invention, so that those skilled in the art may better understand the invention and practice it.
Example 1, material Source of inner Jiang Tiandong root tuber
The material is obtained by separating the tubers of the excellent parent strain, and the specific method is as follows: selecting high-quality inner Jiang Tiandong seedlings which are cultivated for more than 2 years and grow well, shearing off the radix asparagi tubers by scissors, selecting the tubers which are complete, have no obvious damage and have proper sizes at the two ends of the tubers, and removing the over-small radix asparagi tubers, wherein the result is shown in figure 1. Then the whole root tuber is put into 0.1 percent carbendazim solution to be soaked for 10 minutes, and the whole root tuber is put into a ventilation place to be dried in shade for one day after being washed by water; spreading the tubers on the culture medium according to rows, covering the culture medium by about 5cm, watering, erecting a sunshade net, and setting the sunshade rate to be 50-75 ℃ and the temperature to be 25-30 ℃. In this example, the culture medium was prepared as vegetable garden soil: peat soil: vermiculite: perlite=3:3:3:0.5, and finally adding 0.01% carbendazim into soil to reduce germs; the thickness of the culture medium on the seedbed is 10-15cm.
Example 2 establishment of inner Jiang Tiandong root tuber cultivation System
In order to select tubers with proper size and position as breeding materials, the regeneration capacities of different tubers of the asparagus cochinchinensis are compared, and the specific method is as follows:
grading according to the size of the root tuber, wherein 5-7g of the root tuber is a four-stage root tuber, 9-10g of a three-stage root tuber, 12-13g of the root tuber is a two-stage root tuber, and 18-19g of the root tuber is a one-stage root tuber. Comparing the graded tuberous roots with different sizes as materials, and researching the influence of the different tuberous root sizes on seedling formation; taking the largest secondary tuberous roots as materials, respectively removing the upper end, removing the lower end, and comparing the materials at the two removed ends to study the influence of different damage conditions of the tuberous roots on seedling. The specific conditions are shown in Table 1:
table 1, cultivation of inner Jiang Tiandong root tuber propagation System
The result shows that the seedlings can be grown by taking the tuberous roots with different sizes, the weight of the seedlings and the size of the tuberous roots form a certain positive correlation, and the maximum newly-grown tuberous root length and the size of the old tuberous roots also show a certain positive correlation. Secondly, in comparison of different deletions of the root tuber, the inner Jiang Tiandong is difficult to seedling when the two ends are removed; the seedling is difficult to form even if the lower end is deleted; the upper end of the deletion has little influence on the seedling of the asparagus; in Jiang Tiandong observation without deletion, emergence and root formation are also almost from the morphological lower part of the root tuber; next, the bud is formed on the upward side of the root tuber, and the root tuber is formed on the downward side, and the related picture is shown in fig. 1.
Taking the root tuber of the inner Jiang Tiandong as a material, sterilizing and airing, and growing new roots and stems and leaves on a culture medium about 60; and forming an asparagus seedling around 180d, wherein the related picture is shown in figure 2.
The above-described embodiments are merely preferred embodiments for fully explaining the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutions and modifications will occur to those skilled in the art based on the present invention, and are intended to be within the scope of the present invention. The protection scope of the invention is subject to the claims.
The invention discloses a breeding method of inner Jiang Tiandong tubers, which comprises the specific steps of taking down the inner Jiang Tiandong tubers, taking care of not damaging the tubers, sterilizing by using carbendazim, and airing overnight; then covering the dried root tuber with culture medium with 5cm thickness, watering fully, inducing leaf buds, and rooting; the seedling stage is careful to shade and retain water; the method is simple, can obtain a large number of seedlings of Nei Jiang Tiandong in half a year, and has important significance for artificial planting of Nei Jiang radix asparagi.
The above-described embodiments are merely preferred embodiments for fully explaining the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutions and modifications will occur to those skilled in the art based on the present invention, and are intended to be within the scope of the present invention. The protection scope of the invention is subject to the claims.
Claims (4)
1. The large-scale breeding method of the radix asparagi in the river based on the tuberous root is characterized by comprising the following steps:
(1) Selecting an inner Jiang Tiandong with a cultivation period of more than 2 years and strong growth as a mother plant, and selecting root tubers with complete roots at two ends of the root tubers, no obvious damage and proper size;
(2) Soaking the tuberous root in carbendazim solution, washing with water, and drying in shade at ventilation place;
(3) Preparing a seedbed by selecting well-drained soil, and paving a culture medium on a common Miao Chuangshang with the thickness of 10-15cm;
(4) Spreading the tuberous root on a culture medium according to row arrangement, covering the culture medium by about 5cm, finally watering, erecting a sunshade net, wherein the sunshade rate is 50-75%, and the temperature is 25-30 ℃.
2. The root tuber-based large-scale breeding method for radix asparagi in river, which is characterized in that: the culture medium is prepared from vegetable garden soil: peat soil: vermiculite: the mass ratio of the perlite is 3:3:3:0.5.
3. The root tuber-based large-scale breeding method for radix asparagi in river, which is characterized in that: the root tuber size is 18-19g.
4. The root tuber-based large-scale breeding method for radix asparagi in river, which is characterized in that: the carbendazim is soaked in a carbendazim solution with the mass fraction of 0.1% for 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311701065.4A CN117461530A (en) | 2023-12-12 | 2023-12-12 | Root tuber-based large-scale breeding method for radix asparagi in river |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311701065.4A CN117461530A (en) | 2023-12-12 | 2023-12-12 | Root tuber-based large-scale breeding method for radix asparagi in river |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117461530A true CN117461530A (en) | 2024-01-30 |
Family
ID=89627690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311701065.4A Pending CN117461530A (en) | 2023-12-12 | 2023-12-12 | Root tuber-based large-scale breeding method for radix asparagi in river |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117461530A (en) |
-
2023
- 2023-12-12 CN CN202311701065.4A patent/CN117461530A/en active Pending
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