CN117256730A - Probiotic-containing feed additive for conditioning animal intestinal tracts and preparation method thereof - Google Patents
Probiotic-containing feed additive for conditioning animal intestinal tracts and preparation method thereof Download PDFInfo
- Publication number
- CN117256730A CN117256730A CN202311567032.5A CN202311567032A CN117256730A CN 117256730 A CN117256730 A CN 117256730A CN 202311567032 A CN202311567032 A CN 202311567032A CN 117256730 A CN117256730 A CN 117256730A
- Authority
- CN
- China
- Prior art keywords
- feed additive
- preparation
- component
- powder
- probiotic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 77
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 77
- 239000003674 animal food additive Substances 0.000 title claims abstract description 75
- 241001465754 Metazoa Species 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 57
- 210000001035 gastrointestinal tract Anatomy 0.000 title claims abstract description 54
- 230000003750 conditioning effect Effects 0.000 title claims abstract description 35
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 33
- 230000004048 modification Effects 0.000 claims abstract description 19
- 238000012986 modification Methods 0.000 claims abstract description 19
- 230000008569 process Effects 0.000 claims abstract description 15
- 239000000306 component Substances 0.000 claims description 78
- 239000007788 liquid Substances 0.000 claims description 73
- 239000000843 powder Substances 0.000 claims description 61
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 50
- 238000003756 stirring Methods 0.000 claims description 39
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 36
- 239000000126 substance Substances 0.000 claims description 34
- 239000000463 material Substances 0.000 claims description 33
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 30
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 30
- 239000008367 deionised water Substances 0.000 claims description 30
- 229910021641 deionized water Inorganic materials 0.000 claims description 30
- 239000007789 gas Substances 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 28
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 claims description 28
- 238000001179 sorption measurement Methods 0.000 claims description 27
- 238000010438 heat treatment Methods 0.000 claims description 24
- 239000007787 solid Substances 0.000 claims description 23
- 241000194032 Enterococcus faecalis Species 0.000 claims description 21
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 21
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 21
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 21
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 21
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 21
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 claims description 20
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 20
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 19
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 19
- SZQUEWJRBJDHSM-UHFFFAOYSA-N iron(3+);trinitrate;nonahydrate Chemical compound O.O.O.O.O.O.O.O.O.[Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O SZQUEWJRBJDHSM-UHFFFAOYSA-N 0.000 claims description 18
- YZYKBQUWMPUVEN-UHFFFAOYSA-N zafuleptine Chemical compound OC(=O)CCCCCC(C(C)C)NCC1=CC=C(F)C=C1 YZYKBQUWMPUVEN-UHFFFAOYSA-N 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 16
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 16
- 239000011162 core material Substances 0.000 claims description 16
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 16
- 235000013628 Lantana involucrata Nutrition 0.000 claims description 15
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 claims description 15
- 102000016943 Muramidase Human genes 0.000 claims description 15
- 108010014251 Muramidase Proteins 0.000 claims description 15
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 15
- 240000007673 Origanum vulgare Species 0.000 claims description 15
- 150000001875 compounds Chemical class 0.000 claims description 15
- 238000001816 cooling Methods 0.000 claims description 15
- 235000019441 ethanol Nutrition 0.000 claims description 15
- 239000004325 lysozyme Substances 0.000 claims description 15
- 235000010335 lysozyme Nutrition 0.000 claims description 15
- 229960000274 lysozyme Drugs 0.000 claims description 15
- 238000004321 preservation Methods 0.000 claims description 14
- 238000000498 ball milling Methods 0.000 claims description 13
- 238000000576 coating method Methods 0.000 claims description 13
- 230000005855 radiation Effects 0.000 claims description 13
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 11
- 229940009289 bifidobacterium lactis Drugs 0.000 claims description 11
- 239000011248 coating agent Substances 0.000 claims description 11
- 238000013329 compounding Methods 0.000 claims description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 241000186000 Bifidobacterium Species 0.000 claims description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 10
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 10
- 229920001100 Polydextrose Polymers 0.000 claims description 10
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 239000001259 polydextrose Substances 0.000 claims description 10
- 235000013856 polydextrose Nutrition 0.000 claims description 10
- 229940035035 polydextrose Drugs 0.000 claims description 10
- 239000000661 sodium alginate Substances 0.000 claims description 10
- 235000010413 sodium alginate Nutrition 0.000 claims description 10
- 229940005550 sodium alginate Drugs 0.000 claims description 10
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 10
- 238000000227 grinding Methods 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 210000000582 semen Anatomy 0.000 claims description 9
- 240000006499 Flammulina velutipes Species 0.000 claims description 8
- 235000016640 Flammulina velutipes Nutrition 0.000 claims description 8
- 244000037364 Cinnamomum aromaticum Species 0.000 claims description 6
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 6
- 238000001354 calcination Methods 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 4
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 4
- 238000002203 pretreatment Methods 0.000 claims description 2
- 238000001291 vacuum drying Methods 0.000 claims 2
- 238000011534 incubation Methods 0.000 claims 1
- 230000035484 reaction time Effects 0.000 claims 1
- 230000000630 rising effect Effects 0.000 claims 1
- 238000003860 storage Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 239000002253 acid Substances 0.000 abstract description 5
- 230000002779 inactivation Effects 0.000 abstract description 2
- 206010012735 Diarrhoea Diseases 0.000 description 12
- 238000000605 extraction Methods 0.000 description 12
- 235000016709 nutrition Nutrition 0.000 description 12
- 230000035764 nutrition Effects 0.000 description 12
- 239000002245 particle Substances 0.000 description 12
- 238000011160 research Methods 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 10
- 239000003814 drug Substances 0.000 description 10
- 238000001035 drying Methods 0.000 description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 7
- 230000000968 intestinal effect Effects 0.000 description 7
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 210000001744 T-lymphocyte Anatomy 0.000 description 5
- 210000003719 b-lymphocyte Anatomy 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000001132 ultrasonic dispersion Methods 0.000 description 4
- 239000003054 catalyst Substances 0.000 description 3
- 235000012000 cholesterol Nutrition 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 108010047620 Phytohemagglutinins Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 210000002249 digestive system Anatomy 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000004064 dysfunction Effects 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000001885 phytohemagglutinin Effects 0.000 description 2
- -1 polytetrafluoroethylene Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000282887 Suidae Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000020980 bad eating habits Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 210000005027 intestinal barrier Anatomy 0.000 description 1
- 230000007358 intestinal barrier function Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a probiotic-containing feed additive for conditioning animal intestinal tracts and a preparation method thereof, and belongs to the technical field of feed additives. The preparation method of the probiotic-containing feed additive for conditioning animal intestinal tracts comprises the following steps: pretreatment, modification treatment, preparation of a first component, preparation of a second component and preparation. The preparation method of the feed additive containing probiotics for conditioning animal intestinal tracts can effectively improve the stability of probiotics in the feed additive, improve the heat resistance and acid resistance of the feed additive, effectively avoid the inactivation of the probiotics in the preparation and application processes of the feed additive, and effectively regulate the animal intestinal tract environment, and has good application effect and low application amount; meanwhile, the storage stability of the feed additive containing probiotics in a higher temperature environment is further improved.
Description
Technical Field
The invention relates to the field of feed additives, in particular to a probiotic-containing feed additive for conditioning animal intestinal tracts and a preparation method thereof.
Background
Diarrhea is caused by a plurality of reasons in the process of breeding and feeding animals, and bad eating habits, a large amount of antibiotics and the like can cause unbalanced intestinal flora of the animals so as to damage intestines and stomach. The intestinal tract is used as the largest immune organ of the animal body, plays an important role in the aspect of immunizing external pathogens, once the intestinal tract is damaged, the intestinal tract dysfunction is very easy to occur, and the problems of poor absorption of nutrient substances by the intestinal tract and the like are further caused, so that the growth and development of the animal are influenced, and the health of the animal body is also seriously influenced. Meanwhile, after the animals undergo surgery or after the animals are weaned, the problems of intestinal dysfunction, intestinal damage and the like are very easy to occur due to the fact that an autoimmune system and an intestinal system are fragile.
For the foregoing cases, probiotics are often used to regulate the animal's intestinal tract; or the probiotics are adopted to regulate the intestinal environment of animals when being matched with the treatment medicines such as antibiotics and the like for treatment. The conventional use method of probiotics in the prior art is that a proper amount of probiotics is added into animal feed grains, and after the probiotics are ingested by animals, the probiotics reach intestinal tracts along with the food through a digestive system, and can be planted on intestinal mucosa to repair damaged intestinal barriers, occupy epithelial cells of the intestinal mucosa, inhibit invasion and planting of pathogenic bacteria, stimulate an immune system to generate various antibodies, and further improve the immunity of the animals; meanwhile, the flora balance in the intestinal tracts of animals is regulated, a good living environment is provided for beneficial flora, the digestion and absorption of nutrient substances by animal organisms are enhanced, and the growth performance of animals is improved.
However, in the process of preparing the feed additive containing probiotics with the intestinal tract conditioning function, as the probiotics are low in stability, poor in heat resistance and acid resistance, the probiotics in the feed additive cannot effectively reach the intestinal tract due to the influence of complex process conditions, gastric acid in the animal digestion process or other raw material components in the feed, and the due conditioning function of the probiotics cannot be realized, so that the application effect of the feed additive is not ideal, and the application amount is large.
Meanwhile, the storage stability of the feed additive containing probiotics in a higher temperature environment is not ideal, and the probiotics are easy to inactivate in the long-term storage process and cannot be stored stably for a long time.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides the probiotic-containing feed additive for conditioning animal intestinal tracts and the preparation method thereof, which can effectively improve the stability of probiotics in the feed additive, improve the heat resistance and acid resistance of the probiotic, effectively avoid the inactivation of the probiotics in the preparation and application processes of the feed additive, effectively regulate the animal intestinal tract environment, and have good application effect and low application amount; meanwhile, the storage stability of the feed additive containing probiotics in a higher temperature environment is further improved.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a preparation method of a feed additive containing probiotics for conditioning animal intestinal tracts comprises the following steps: pretreatment, modification treatment, preparation of a first component, preparation of a second component and preparation.
The pretreatment method comprises the steps of adding zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid into an ethanol solution, and stirring until the zinc acetate dihydrate, the ferric nitrate nonahydrate and the malonic acid are completely dissolved to prepare an adsorption liquid; then putting mesoporous activated carbon into an adsorption liquid, stirring at normal temperature for 2-3h after ultrasonic dispersion is uniform, adding a pretreatment liquid, mixing uniformly, transferring into a lining polytetrafluoroethylene autoclave, sealing the autoclave, heating to 100-110 ℃, preserving heat for 5-6h, naturally cooling to room temperature, filtering out solid matters, washing the solid matters with absolute ethyl alcohol with 1.2-1.5 times of volume for 2-3 times, placing into a vacuum incubator, drying to constant weight at 80-90 ℃ in an environment with the vacuum degree of 0.08-0.09MPa, and grinding uniformly to obtain the pretreatment matters.
In the pretreatment, the weight ratio of zinc acetate dihydrate, ferric nitrate nonahydrate, malonic acid and ethanol solution in the adsorption liquid is 0.8-1:1.6-2:0.4-0.45:80-90.
The volume concentration of the ethanol solution in the adsorption liquid is 30-35%;
the pretreatment liquid is an N, N-dimethylformamide solution in which terephthalic acid is dissolved; the preparation method is that the terephthalic acid is added into N, N-dimethylformamide with the weight of 50 to 55 times, and the mixture is stirred and dissolved to prepare the catalyst.
In the pretreatment, the volume ratio of the mesoporous activated carbon to the adsorption liquid is 1:2-2.5;
the volume ratio of the adsorption liquid to the pretreatment liquid is 1:0.3-0.4.
The modification treatment method comprises the steps of placing a pretreatment object in a closed environment, adopting mixed gas to replace air in the closed environment completely, continuously introducing the mixed gas at an introducing rate of 1.5-2L/min, heating to 180-220 ℃ by microwave radiation, performing intermittent microwave heat preservation for 50-60min, naturally cooling to room temperature, transferring into a calciner, heating to 500-550 ℃ in the mixed gas environment at a heating rate of 2-3 ℃/min, performing heat preservation calcination for 3-4h, naturally cooling to room temperature, and grinding uniformly to obtain the modified treatment object.
In the modification treatment, the mixed gas is a mixed gas of nitrogen and oxygen, and the volume percentage content of the oxygen in the mixed gas is 22-23%;
the microwave radiation frequency is 2400-2500MHz, and the microwave power is 1.2-1.3KW.
The preparation of the first component consists of the following steps: compounding and coating.
The compounding method comprises the steps of putting the modified treatment substance, light calcium carbonate and starch into a dry ball mill according to the weight ratio of 10-12:8-10:3-4, controlling the ball-material ratio to be 5-6:1, and performing ball milling at the rotating speed of 150-200rpm for 10-20min to obtain a ball-milled substance; and then uniformly mixing the ball-milled material with bifidobacterium powder, lactobacillus plantarum powder, lactobacillus acidophilus powder and enterococcus faecalis powder to prepare the compound.
In the compounding, the weight ratio of the ball milling material to the bifidobacterium powder to the lactobacillus plantarum powder to the lactobacillus acidophilus powder to the enterococcus faecalis powder is 80-85:3-4:4-4.5:2-3:1-1.5;
the effective bacterial concentration of the bifidobacterium lactis bacterial powder is more than or equal to 100 hundred million cfu/g;
the effective bacterial concentration of the lactobacillus plantarum bacterial powder is more than or equal to 100 hundred million cfu/g;
the effective bacteria concentration of lactobacillus acidophilus bacteria powder is more than or equal to 100 hundred million cfu/g;
the effective bacterial concentration of the enterococcus faecalis bacterial powder is more than or equal to 1000 hundred million cfu/g.
The coating method comprises the steps of adding sodium alginate and carboxymethyl cellulose into deionized water at 50-60 ℃ under stirring, preserving heat, stirring for 2-3h, and obtaining wall material liquid after ultrasonic defoaming; under the stirring condition, putting the compound, glycerol and sodium dodecyl sulfate into deionized water, uniformly dispersing, and stirring for 20-30min to obtain core material liquid; uniformly mixing the core material liquid and the wall material liquid according to the volume ratio of 1:1.5-2, spraying into 3-4 times of calcium chloride solution, stirring for 60-90min, centrifuging at 10000-12000rpm to separate solid matters, and freeze-drying to obtain a first component.
In the coating, the weight ratio of sodium alginate, carboxymethyl cellulose and deionized water in the wall material liquid is 1-1.2:0.1-0.15:100-110;
the weight ratio of the compound to the glycerol to the sodium dodecyl sulfate to the deionized water in the core material liquid is 18-20:4-5:0.2-0.3:100-110;
the concentration of the calcium chloride solution is 0.1-0.15mol/L.
The method for preparing the second component comprises the steps of adding flammulina velutipes extract, cassia seed extract and oregano extract into deionized water, and uniformly dispersing to prepare an impregnating solution; and (3) adding the modified treatment substance into impregnating solution with the weight being 5-6 times that of the modified treatment substance, heating to 40-45 ℃, preserving heat, stirring for 4-5 hours, filtering out solid substances, placing into a vacuum incubator, and drying for 6-7 hours at 75-85 ℃ in an environment with the vacuum degree of 0.08-0.09MPa to obtain a second component.
In the preparation of the second component, the weight ratio of flammulina velutipes extract, cassia seed extract, oregano extract and deionized water in the impregnating solution is 3.5-4:2-2.5:0.8-1:100-110;
the particle size of the needle mushroom extract is 80-100 meshes, and the extraction ratio is 10-20:1;
the particle size of the semen cassiae extract is 80-100 meshes, and the extraction ratio is 10-20:1;
the particle size of the oregano extract is 80-100 meshes, and the extraction ratio is 10-20:1.
The preparation is prepared by uniformly mixing the first component, the second component, lysozyme, polydextrose and sodium carboxymethyl cellulose, granulating, and preparing the feed additive containing probiotics for regulating animal intestinal tracts.
In the preparation, the weight ratio of the first component to the second component to the lysozyme to the polydextrose to the sodium carboxymethylcellulose is 40-50:25-30:0.5-0.6:18-20:0.1-0.15.
A probiotic-containing feed additive for conditioning animal intestinal tracts is prepared by adopting the preparation method.
Compared with the prior art, the invention has the beneficial effects that:
(1) The preparation method of the probiotic-containing feed additive for conditioning animal intestinal tracts comprises the steps of preparing a double-ligand metal frame material by adopting zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid and terephthalic acid in a pretreatment step, and compounding mesoporous activated carbon in the pretreatment process; in the modification treatment step, the pretreatment substance is activated by microwave radiation and then is calcined, carbonized and modified to prepare a modified treatment substance; combining the modified treated matter with the effective component of the first component, and compounding and coating to obtain the first component; combining the modified treatment substance with the effective component of the second component to prepare the second component; then preparing a probiotic-containing feed additive for regulating the intestinal tracts of animals by using the first component, the second component, lysozyme and other matched preparations; the stability of probiotics in the feed additive can be effectively improved, the heat resistance and acid resistance of the probiotics are improved, the probiotics are effectively prevented from being inactivated in the preparation and application processes of the feed additive, the feed additive containing the probiotics can effectively regulate the intestinal environment of animals, the application effect is good, and the application amount is low; meanwhile, the storage stability of the feed additive containing probiotics in a higher temperature environment is further improved.
(2) Experiments prove that the feed additive containing probiotics for conditioning animal intestinal tracts is added into daily feed of laying hens in an amount of 1.5wt%, the laying rate of the laying hens is 89.2-90.2%, the average egg weight is 61.0-61.4g, the daily average feed intake is 109.6-112.0g, the death rate is 0%, and the diarrhea rate is 0.36-0.40%.
(3) Through experiments, the probiotic-containing feed additive for conditioning animal intestinal tracts is added into daily feed of laying hens in an amount of 1.5wt%, the T lymphocyte conversion rate of the laying hens is 78.0-78.4%, the B lymphocyte conversion rate is 83.4-85.0%, and the cholesterol content is 3.67-3.74mmol/L.
(4) Through experiments, the probiotic-containing feed additive for conditioning animal intestinal tracts is added into weaned piglet daily ration in an amount of 1.8wt%, the daily gain of piglets is 492-501g/d, the feed conversion ratio is 1.57-1.58, and the diarrhea rate is 0.48-0.52%.
(5) Through experiments, the survival rate of the probiotics-containing feed additive for conditioning animal intestinal tracts is 96-98% after the probiotics-containing feed additive is stood for 180d in an environment with the temperature of 42 ℃ and the relative humidity of 65%.
Detailed Description
Specific embodiments of the present invention will now be described in order to provide a clearer understanding of the technical features, objects and effects of the present invention.
Example 1
The embodiment provides a preparation method of a feed additive containing probiotics for conditioning animal intestinal tracts, which specifically comprises the following steps:
1. pretreatment of
Adding zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid into an ethanol solution, and stirring until the zinc acetate dihydrate, the ferric nitrate nonahydrate and the malonic acid are completely dissolved to prepare an adsorption liquid; then putting mesoporous activated carbon into an adsorption liquid, stirring for 2 hours at normal temperature after ultrasonic dispersion is uniform, adding a pretreatment liquid, mixing uniformly, transferring into a polytetrafluoroethylene-lined autoclave, sealing the autoclave, heating to 100 ℃, preserving heat for 5 hours, naturally cooling to room temperature, filtering out solid matters, washing the solid matters with absolute ethyl alcohol with the volume of 1.2 times for 2 times, placing into a vacuum incubator, drying to constant weight at 80 ℃ in the vacuum degree of 0.08MPa, and grinding uniformly to obtain the pretreated matters.
In the adsorption liquid, the weight ratio of zinc acetate dihydrate to ferric nitrate nonahydrate to malonic acid to ethanol solution is 0.8:1.6:0.4:80.
The volume concentration of the ethanol solution was 30%.
The pretreatment liquid is an N, N-dimethylformamide solution in which terephthalic acid is dissolved; the preparation method is that terephthalic acid is added into N, N-dimethylformamide with the weight of 50 times, and the mixture is stirred and dissolved to prepare the catalyst.
The volume ratio of the mesoporous activated carbon to the adsorption liquid is 1:2.
The volume ratio of the adsorption liquid to the pretreatment liquid is 1:0.3.
2. Modification treatment
Placing the pretreatment in a closed environment, adopting mixed gas to replace air in the closed environment completely, continuously introducing the mixed gas at an introducing rate of 1.5L/min, heating to 180 ℃ by microwave radiation, performing intermittent microwave heat preservation for 50min, naturally cooling to room temperature, transferring into a calciner, heating to 500 ℃ at a heating rate of 2 ℃/min in the mixed gas environment, performing heat preservation and calcination for 3h, naturally cooling to room temperature, and grinding uniformly to obtain the modified treatment.
Wherein the mixed gas is a mixed gas of nitrogen and oxygen, and the oxygen content in the mixed gas is 22 percent (volume percent).
The microwave radiation frequency is 2400MHz, and the microwave power is 1.2KW.
3. Preparation of the first component
1) Composite material
Putting the modified treated matter, light calcium carbonate and starch into a dry ball mill according to the weight ratio of 10:8:3, controlling the ball-material ratio to be 5:1, and performing ball milling at a ball milling rotating speed of 150rpm for 10min to obtain a ball-milled matter; and then uniformly mixing the ball-milled material with bifidobacterium powder, lactobacillus plantarum powder, lactobacillus acidophilus powder and enterococcus faecalis powder to prepare the compound.
Wherein the weight ratio of the ball-milling material to the bifidobacterium powder to the lactobacillus plantarum powder to the lactobacillus acidophilus powder to the enterococcus faecalis powder is 80:3:4:2:1.
Bifidobacterium lactis, latin name Bifidobacterium lactis, and Bifidobacterium lactis powder are purchased from the micro-ecological research institute of nutrition science (Shandong) of the middle science, and the effective bacteria concentration is more than or equal to 100 hundred million cfu/g.
Lactobacillus plantarum, latin name Lactobacillus plantarum, and Lactobacillus plantarum powder are purchased from Shankai Jiayi nutrition medicine (Shandong) micro-ecological research institute Limited company, and the effective bacteria concentration is more than or equal to 100 hundred million cfu/g.
Lactobacillus acidophilus, latin name Lactobacillus acidophilus, and Lactobacillus acidophilus powder purchased from Shankai Jiayi nutrition medicine (Shandong) micro-ecological research institute Co., ltd, wherein the effective bacteria concentration is not less than 100 hundred million cfu/g.
Enterococcus faecalis, latin name Enterococcus faecalis, and enterococcus faecalis bacterial powder purchased from the micro ecological research institute of sciences of Jia Yi nutrition medicine (Shandong) of the middle department, wherein the effective bacterial concentration is more than or equal to 1000 hundred million cfu/g.
2) Coating
Under the stirring condition, sodium alginate and carboxymethyl cellulose are put into deionized water with the temperature of 50 ℃, and are stirred for 2 hours under heat preservation, and wall material liquid is obtained after ultrasonic defoaming; under the stirring condition, putting the compound, glycerol and sodium dodecyl sulfate into deionized water, uniformly dispersing, and stirring for 20min to obtain core material liquid; uniformly mixing the core material liquid and the wall material liquid according to the volume ratio of 1:1.5, spraying into a calcium chloride solution with the volume being 3 times, stirring for 60min, centrifuging at 10000rpm to separate solid matters, and freeze-drying to obtain a first component.
Wherein, in the wall material liquid, the weight ratio of the sodium alginate to the carboxymethyl cellulose to the deionized water is 1:0.1:100.
In the core material liquid, the weight ratio of the compound to the glycerol to the sodium dodecyl sulfate to the deionized water is 18:4:0.2:100.
The concentration of the calcium chloride solution was 0.1mol/L.
4. Preparation of the second component
Adding needle mushroom extract, semen Cassiae extract and Oregano extract into deionized water, and dispersing uniformly to obtain soaking solution; and (3) adding the modified treatment substance into the impregnating solution with the weight being 5 times that of the modified treatment substance, heating to 40 ℃, preserving heat and stirring for 4 hours, filtering out solid substances, placing the solid substances in a vacuum incubator, and drying at 75 ℃ for 6 hours in an environment with the vacuum degree of 0.08MPa to obtain the second component.
In the impregnating solution, the weight ratio of the flammulina velutipes extract to the cassia seed extract to the oregano extract to the deionized water is 3.5:2:0.8:100.
The particle size of the needle mushroom extract is 80 meshes, and the extraction ratio is 10:1.
The particle size of the semen cassiae extract is 80 meshes, and the extraction ratio is 10:1.
The particle size of the oregano extract is 80 meshes, and the extraction ratio is 10:1.
5. Formulations
Uniformly mixing the first component, the second component, lysozyme, polydextrose and sodium carboxymethyl cellulose, and granulating to obtain the feed additive containing probiotics for regulating animal intestinal tracts.
Wherein the weight ratio of the first component to the second component to the lysozyme to the polydextrose to the sodium carboxymethylcellulose is 40:25:0.5:18:0.1.
The embodiment also provides a probiotic-containing feed additive for conditioning animal intestinal tracts, which is prepared by adopting the preparation method.
Example 2
The embodiment provides a preparation method of a feed additive containing probiotics for conditioning animal intestinal tracts, which specifically comprises the following steps:
1. pretreatment of
Adding zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid into an ethanol solution, and stirring until the zinc acetate dihydrate, the ferric nitrate nonahydrate and the malonic acid are completely dissolved to prepare an adsorption liquid; then putting mesoporous activated carbon into an adsorption liquid, stirring at normal temperature for 2.5 hours after ultrasonic dispersion is uniform, adding a pretreatment liquid, mixing uniformly, transferring into a lining polytetrafluoroethylene autoclave, sealing the autoclave, heating to 105 ℃, preserving heat for 5.5 hours, naturally cooling to room temperature, filtering out solid matters, washing the solid matters with absolute ethyl alcohol with the volume of 1.3 times for 3 times, placing into a vacuum incubator, drying at 85 ℃ to constant weight in the environment with the vacuum degree of 0.085MPa, and grinding uniformly to obtain the pretreatment matters.
In the adsorption liquid, the weight ratio of zinc acetate dihydrate to ferric nitrate nonahydrate to malonic acid to ethanol solution is 0.9:1.8:0.42:85.
The volume concentration of the ethanol solution was 32%.
The pretreatment liquid is an N, N-dimethylformamide solution in which terephthalic acid is dissolved; the preparation method is that terephthalic acid is put into N, N-dimethylformamide with 53 times of weight, and the terephthalic acid is obtained by stirring and dissolving.
The volume ratio of the mesoporous activated carbon to the adsorption liquid is 1:2.2.
The volume ratio of the adsorption liquid to the pretreatment liquid is 1:0.35.
2. Modification treatment
Placing the pretreatment in a closed environment, adopting mixed gas to replace air in the closed environment completely, continuously introducing the mixed gas at an introducing rate of 1.8L/min, heating to 190 ℃ by microwave radiation, carrying out intermittent microwave heat preservation for 55min, naturally cooling to room temperature, transferring into a calciner, heating to 520 ℃ in the mixed gas environment at a heating rate of 2.5 ℃/min, carrying out heat preservation and calcination for 3.5h, naturally cooling to room temperature, and grinding uniformly to obtain the modified treatment.
Wherein the mixed gas is a mixed gas of nitrogen and oxygen, and the oxygen content in the mixed gas is 22.5 percent (volume percent).
The microwave radiation frequency is 2450MHz and the microwave power is 1.25KW.
3. Preparation of the first component
1) Composite material
Putting the modified treated matter, light calcium carbonate and starch into a dry ball mill according to the weight ratio of 11:9:3.5, controlling the ball-material ratio to be 5.5:1, and performing ball milling at the ball milling rotating speed of 170rpm for 15min to obtain a ball-milled matter; and then uniformly mixing the ball-milled material with bifidobacterium powder, lactobacillus plantarum powder, lactobacillus acidophilus powder and enterococcus faecalis powder to prepare the compound.
Wherein the weight ratio of the ball-milling material to the bifidobacterium powder to the lactobacillus plantarum powder to the lactobacillus acidophilus powder to the enterococcus faecalis powder is 82:3.5:4.2:2.5:1.3.
Bifidobacterium lactis, latin name Bifidobacterium lactis, and Bifidobacterium lactis powder are purchased from the micro-ecological research institute of nutrition science (Shandong) of the middle science, and the effective bacteria concentration is more than or equal to 100 hundred million cfu/g.
Lactobacillus plantarum, latin name Lactobacillus plantarum, and Lactobacillus plantarum powder are purchased from Shankai Jiayi nutrition medicine (Shandong) micro-ecological research institute Limited company, and the effective bacteria concentration is more than or equal to 100 hundred million cfu/g.
Lactobacillus acidophilus, latin name Lactobacillus acidophilus, and Lactobacillus acidophilus powder purchased from Shankai Jiayi nutrition medicine (Shandong) micro-ecological research institute Co., ltd, wherein the effective bacteria concentration is not less than 100 hundred million cfu/g.
Enterococcus faecalis, latin name Enterococcus faecalis, and enterococcus faecalis bacterial powder purchased from the micro ecological research institute of sciences of Jia Yi nutrition medicine (Shandong) of the middle department, wherein the effective bacterial concentration is more than or equal to 1000 hundred million cfu/g.
2) Coating
Under the stirring condition, sodium alginate and carboxymethyl cellulose are put into deionized water with the temperature of 55 ℃, and are stirred for 2.5 hours under heat preservation, and wall material liquid is obtained after ultrasonic defoaming; under the stirring condition, putting the compound, glycerol and sodium dodecyl sulfate into deionized water, uniformly dispersing, and stirring for 25min to obtain core material liquid; uniformly mixing the core material liquid and the wall material liquid according to the volume ratio of 1:1.8, spraying into a calcium chloride solution with the volume being 3.5 times, stirring for 75 minutes, centrifuging at 11000rpm to separate out solid matters, and freeze-drying to obtain a first component.
Wherein, in the wall material liquid, the weight ratio of the sodium alginate to the carboxymethyl cellulose to the deionized water is 1.1:0.12:105.
In the core material liquid, the weight ratio of the compound to the glycerol to the sodium dodecyl sulfate to the deionized water is 19:4.5:0.25:105.
The concentration of the calcium chloride solution was 0.12mol/L.
4. Preparation of the second component
Adding needle mushroom extract, semen Cassiae extract and Oregano extract into deionized water, and dispersing uniformly to obtain soaking solution; and (3) adding the modified treatment substance into the impregnating solution with the weight being 5.5 times that of the modified treatment substance, heating to 42 ℃, preserving heat and stirring for 4.5 hours, filtering out solid substances, placing the solid substances in a vacuum incubator, and drying at 80 ℃ for 6.5 hours in an environment with the vacuum degree of 0.085MPa to obtain a second component.
In the impregnating solution, the weight ratio of the flammulina velutipes extract to the cassia seed extract to the oregano extract to the deionized water is 3.8:2.3:0.9:105.
The particle size of the needle mushroom extract is 90 meshes, and the extraction ratio is 20:1.
The particle size of the semen cassiae extract is 90 meshes, and the extraction ratio is 20:1.
The particle size of the oregano extract is 90 meshes, and the extraction ratio is 20:1.
5. Formulations
Uniformly mixing the first component, the second component, lysozyme, polydextrose and sodium carboxymethyl cellulose, and granulating to obtain the feed additive containing probiotics for regulating animal intestinal tracts.
Wherein the weight ratio of the first component to the second component to the lysozyme to the polydextrose to the sodium carboxymethylcellulose is 45:28:0.55:19:0.13.
The embodiment also provides a probiotic-containing feed additive for conditioning animal intestinal tracts, which is prepared by adopting the preparation method.
Example 3
The embodiment provides a preparation method of a feed additive containing probiotics for conditioning animal intestinal tracts, which specifically comprises the following steps:
1. pretreatment of
Adding zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid into an ethanol solution, and stirring until the zinc acetate dihydrate, the ferric nitrate nonahydrate and the malonic acid are completely dissolved to prepare an adsorption liquid; then putting mesoporous activated carbon into an adsorption liquid, stirring for 3 hours at normal temperature after ultrasonic dispersion is uniform, adding a pretreatment liquid, mixing uniformly, transferring into a polytetrafluoroethylene-lined autoclave, sealing the autoclave, heating to 110 ℃, preserving heat for 6 hours, naturally cooling to room temperature, filtering out solid matters, washing the solid matters 3 times by using absolute ethyl alcohol with 1.5 times of volume, placing into a vacuum incubator, drying to constant weight at 90 ℃ in an environment with the vacuum degree of 0.09MPa, and grinding uniformly to obtain the pretreatment matters.
In the adsorption liquid, the weight ratio of zinc acetate dihydrate to ferric nitrate nonahydrate to malonic acid to ethanol solution is 1:2:0.45:90.
The volume concentration of the ethanol solution was 35%.
The pretreatment liquid is an N, N-dimethylformamide solution in which terephthalic acid is dissolved; the preparation method is that terephthalic acid is added into N, N-dimethylformamide with 55 times of weight, and the mixture is stirred and dissolved to prepare the catalyst.
The volume ratio of the mesoporous activated carbon to the adsorption liquid is 1:2.5.
The volume ratio of the adsorption liquid to the pretreatment liquid is 1:0.4.
2. Modification treatment
Placing the pretreatment in a closed environment, adopting mixed gas to replace air in the closed environment completely, continuously introducing the mixed gas at an introducing rate of 2L/min, heating to 220 ℃ by microwave radiation, preserving heat for 60min by intermittent microwave, naturally cooling to room temperature, transferring into a calciner, heating to 550 ℃ at a heating rate of 3 ℃/min in the mixed gas environment, preserving heat and calcining for 4h, naturally cooling to room temperature, and grinding uniformly to obtain the modified treatment.
Wherein the mixed gas is a mixed gas of nitrogen and oxygen, and the oxygen content in the mixed gas is 23 percent (volume percent).
The microwave radiation frequency is 2500MHz, and the microwave power is 1.3KW.
3. Preparation of the first component
1) Composite material
Putting the modified treated matter, light calcium carbonate and starch into a dry ball mill according to the weight ratio of 12:10:4, controlling the ball-material ratio to be 6:1, and performing ball milling at the ball milling rotating speed of 200rpm for 20min to obtain a ball-milled matter; and then uniformly mixing the ball-milled material with bifidobacterium powder, lactobacillus plantarum powder, lactobacillus acidophilus powder and enterococcus faecalis powder to prepare the compound.
Wherein the weight ratio of the ball-milling material to the bifidobacterium powder to the lactobacillus plantarum powder to the lactobacillus acidophilus powder to the enterococcus faecalis powder is 85:4:4.5:3:1.5.
Bifidobacterium lactis, latin name Bifidobacterium lactis, and Bifidobacterium lactis powder are purchased from the micro-ecological research institute of nutrition science (Shandong) of the middle science, and the effective bacteria concentration is more than or equal to 100 hundred million cfu/g.
Lactobacillus plantarum, latin name Lactobacillus plantarum, and Lactobacillus plantarum powder are purchased from Shankai Jiayi nutrition medicine (Shandong) micro-ecological research institute Limited company, and the effective bacteria concentration is more than or equal to 100 hundred million cfu/g.
Lactobacillus acidophilus, latin name Lactobacillus acidophilus, and Lactobacillus acidophilus powder purchased from Shankai Jiayi nutrition medicine (Shandong) micro-ecological research institute Co., ltd, wherein the effective bacteria concentration is not less than 100 hundred million cfu/g.
Enterococcus faecalis, latin name Enterococcus faecalis, and enterococcus faecalis bacterial powder purchased from the micro ecological research institute of sciences of Jia Yi nutrition medicine (Shandong) of the middle department, wherein the effective bacterial concentration is more than or equal to 1000 hundred million cfu/g.
2) Coating
Under the stirring condition, sodium alginate and carboxymethyl cellulose are put into deionized water with the temperature of 60 ℃, and are stirred for 3 hours under heat preservation, and wall material liquid is obtained after ultrasonic defoaming; under the stirring condition, putting the compound, glycerol and sodium dodecyl sulfate into deionized water, uniformly dispersing, and stirring for 30min to obtain core material liquid; and uniformly mixing the core material liquid and the wall material liquid according to the volume ratio of 1:2, spraying into a calcium chloride solution with the volume of 4 times, stirring for 90min, centrifuging at 12000rpm to separate solid matters, and freeze-drying to obtain the first component.
Wherein, in the wall material liquid, the weight ratio of the sodium alginate to the carboxymethyl cellulose to the deionized water is 1.2:0.15:110.
In the core material liquid, the weight ratio of the compound to the glycerol to the sodium dodecyl sulfate to the deionized water is 20:5:0.3:110.
The concentration of the calcium chloride solution was 0.15mol/L.
4. Preparation of the second component
Adding needle mushroom extract, semen Cassiae extract and Oregano extract into deionized water, and dispersing uniformly to obtain soaking solution; and (3) adding the modified treatment substance into the impregnating solution with the weight being 6 times that of the modified treatment substance, heating to 45 ℃, preserving heat and stirring for 5 hours, filtering out solid substances, placing the solid substances in a vacuum incubator, and drying at 85 ℃ for 7 hours in an environment with the vacuum degree of 0.09MPa to obtain the second component.
In the impregnating solution, the weight ratio of the flammulina velutipes extract to the cassia seed extract to the oregano extract to the deionized water is 4:2.5:1:110.
The particle size of the needle mushroom extract is 100 meshes, and the extraction ratio is 15:1.
The particle size of the semen cassiae extract is 100 meshes, and the extraction ratio is 15:1.
The particle size of the oregano extract is 100 meshes, and the extraction ratio is 15:1.
5. Formulations
Uniformly mixing the first component, the second component, lysozyme, polydextrose and sodium carboxymethyl cellulose, and granulating to obtain the feed additive containing probiotics for regulating animal intestinal tracts.
Wherein the weight ratio of the first component to the second component to the lysozyme to the polydextrose to the sodium carboxymethylcellulose is 50:30:0.6:20:0.15.
The embodiment also provides a probiotic-containing feed additive for conditioning animal intestinal tracts, which is prepared by adopting the preparation method.
Comparative example 1
The technical scheme of the embodiment 2 is adopted, and the difference is that: omitting the pretreatment step and the modification step, and adopting mesoporous activated carbon to replace the modification treatment substance in the steps of preparing the first component and preparing the second component.
Comparative example 2
The technical scheme of the embodiment 2 is adopted, and the difference is that: 1) In the pretreatment, the addition of ferric nitrate nonahydrate and malonic acid is omitted; 2) The modification treatment step is omitted.
Comparative example 3
The technical scheme of the embodiment 2 is adopted, and the difference is that: 1) In the preparation of the first component, the coating step is omitted; 2) Omitting the step of preparing the second component, and omitting the addition of the second component and lysozyme in the step of preparing.
300 healthy layers (Beijing powder 6 red feather layer) with 40 weeks of age, the weight and the laying rate of which are close, are selected as test objects, and are randomly divided into 6 groups (A1-A6 groups), 50 layers of layers are adopted in each group, 3 layers of H-shaped cage breeding are adopted, and the layers are isolated from each other. The feed additives of examples 1-3 and comparative examples 1-3 were added to the diet of the laying hens of groups A1-A6, respectively, in an amount of 1.5% by weight of the diet of the laying hens, and fed continuously for 60 days. During feeding, the feed is fed and drunk freely, and conventional cleaning, epidemic disease immunity and germ killing work are carried out. In the continuous feeding process, the laying rate, average egg weight, daily average feed intake, death rate and diarrhea rate of each group of laying hens are counted. And (3) on the day 60 of continuous feeding, randomly carrying out vein blood sampling on 10 laying hens in each group, detecting the T lymphocyte conversion rate, the B lymphocyte conversion rate and the cholesterol content of the laying hens, and taking an average value.
The diarrhea rate calculating method comprises the following steps: (number of accumulated diarrhea days of diarrhea layer/number of accumulated feeding days of whole group layer) ×100%.
The T lymphocyte conversion rate and B lymphocyte conversion rate are detected by respectively using Phytohemagglutinin (PHA) and bacterial endotoxin (LPS) as stimulators of T lymphocyte and B lymphocyte, and culturing with trace amount of whole blood 3 H-TdR incorporation was detected.
The specific results are shown in the following table:
furthermore, 120 weaned healthy piglets (the mother line and the father line are all long white pigs) with similar birth date and similar weight and 35 days of age are selected as test objects, and are randomly divided into 6 groups (B1-B6 groups), 20 piglets are separated from each other. The feed additives of examples 1-3 and comparative examples 1-3 were added to the feed ration of groups B1-B6, respectively, in an amount of 1.8% by weight of the piglet ration, and fed continuously for 30 days. During feeding, the feed is fed and drunk freely, and conventional cleaning, epidemic disease immunity and germ killing work are carried out. During feeding, the initial weight, daily gain, feed conversion ratio and diarrhea rate of each piglet in each group are counted and recorded, and an average value is obtained. The diarrhea rate calculating method comprises the following steps: (cumulative number of diarrhea days for diarrhea piglets/cumulative number of feeding days for whole group piglets). Times.100%. The specific results are shown in the following table:
further, after the feed additives of examples 1 to 3 and comparative examples 1 to 3 were fermented and cultured for 48 hours, the number of colonies was counted as a reference base value; then the feed additives of examples 1-3 and comparative examples 1-3 are placed in an environment with the temperature of 42 ℃ and the relative humidity of 65wt%, and after standing and storing for 180 days, the feed additives of examples 1-3 and comparative examples 1-3 after standing and storing are fermented and cultured for 48 hours respectively, the number of bacterial colonies is counted, and the survival rate of the live probiotics is calculated.
The method for calculating the survival rate comprises the following steps: (colony count after fermentation culture of the feed additive after storage at rest/reference basal value) ×100%.
The specific results are shown in the following table:
the preparation method of the probiotic-containing feed additive for conditioning animal intestinal tracts can be seen, the dual-ligand metal framework material is prepared by adopting zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid and terephthalic acid in the pretreatment step, and mesoporous activated carbon is compounded in the pretreatment process; in the modification treatment step, the pretreatment substance is activated by microwave radiation and then is calcined, carbonized and modified to prepare a modified treatment substance; combining the modified treated matter with the effective component of the first component, and compounding and coating to obtain the first component; combining the modified treatment substance with the effective component of the second component to prepare the second component; then preparing a probiotic-containing feed additive for regulating the intestinal tracts of animals by using the first component, the second component, lysozyme and other matched preparations; the stability of probiotics in the feed additive can be effectively improved, the heat resistance and acid resistance of the probiotics are improved, the probiotics are effectively prevented from being inactivated in the preparation and application processes of the feed additive, the feed additive containing the probiotics can effectively regulate the intestinal environment of animals, the application effect is good, and the application amount is low; meanwhile, the storage stability of the feed additive containing probiotics in a higher temperature environment is further improved.
As can be seen from comparative examples 1-2, the preparation method of the probiotic-containing feed additive for conditioning animal intestinal tracts comprises the steps of preparing a dual-ligand metal framework material by adopting zinc acetate dihydrate, ferric nitrate nonahydrate, malonic acid and terephthalic acid in a pretreatment step, and compounding mesoporous activated carbon in the pretreatment process; in the modification treatment step, the pretreatment substance is activated by microwave radiation and then is calcined, carbonized and modified to prepare a modified treatment substance; the probiotic bacteria in the feed additive can be effectively improved in stability, the probiotic bacteria are effectively prevented from being inactivated in the preparation and application processes of the feed additive, and effective components such as the probiotic bacteria in the feed additive can effectively reach intestinal tracts, so that the intestinal tract environment of animals is effectively regulated; and improving the storage stability of the feed additive containing probiotics in a higher temperature environment. The specific expression is that the laying rate of the laying hen and the average egg weight are improved, the death rate, the diarrhea rate and the cholesterol content are reduced, and the storage stability is improved; meanwhile, the improvement of T lymphocyte conversion rate and B lymphocyte conversion rate also shows that the feed additive disclosed by the invention can promote lymphocyte proliferation and conversion and promote and regulate cellular immunity.
As can be seen from comparative example 3, the preparation method of the probiotic-containing feed additive for conditioning animal intestinal tracts of the present invention prepares the first component by combining the modified treatment substance with the active ingredient of the first component, compounding and coating the modified treatment substance; combining the modified treatment substance with the effective component of the second component to prepare the second component; then the first component, the second component, lysozyme and other matched preparations are prepared; the probiotic bacteria in the feed additive can effectively reach animal intestinal tracts, and meanwhile, the probiotic bacteria are matched with the effective components (flammulina velutipes extract, semen cassiae extract, oregano extract), lysozyme and the like in the second component to effectively condition the gastrointestinal digestive system of the animal, so that the application effect of the feed additive is further improved.
The percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A preparation method of a feed additive containing probiotics for conditioning animal intestinal tracts, which is characterized by comprising the following steps: pretreatment, modification treatment, preparation of a first component, preparation of a second component and preparation;
the pretreatment method comprises the steps of putting zinc acetate dihydrate, ferric nitrate nonahydrate and malonic acid into an ethanol solution, stirring and dissolving to prepare an adsorption solution; then adding mesoporous activated carbon into the adsorption liquid, uniformly dispersing, stirring at normal temperature, adding the pretreatment liquid, uniformly mixing, transferring into a polytetrafluoroethylene-lined autoclave, sealing the autoclave, heating to 100-110 ℃, carrying out heat preservation reaction, naturally cooling to room temperature, filtering out solid matters, washing the solid matters with absolute ethyl alcohol, and carrying out vacuum drying to obtain a pretreatment matter;
the pretreatment liquid is an N, N-dimethylformamide solution in which terephthalic acid is dissolved;
the modification treatment method comprises the steps of placing a pretreatment object in a closed environment, heating to 180-220 ℃ in a mixed gas environment by microwave radiation, carrying out intermittent microwave heat preservation, naturally cooling to room temperature, transferring into a calciner, heating to 500-550 ℃ in the mixed gas environment, carrying out heat preservation calcination, naturally cooling to room temperature, and grinding uniformly to obtain a modified treatment object;
the mixed gas is a mixed gas of nitrogen and oxygen;
the preparation of the first component consists of the following steps: compounding and coating;
the method for compounding comprises the steps of ball milling modified treated matters, light calcium carbonate and starch uniformly to obtain ball milled matters; then uniformly mixing the ball-milled material with bifidobacterium powder, lactobacillus plantarum powder, lactobacillus acidophilus powder and enterococcus faecalis powder to prepare a compound;
the coating method comprises the steps of adding sodium alginate and carboxymethyl cellulose into deionized water under stirring, preserving heat, stirring, and defoaming to obtain wall material liquid; under the stirring condition, putting the compound, glycerol and sodium dodecyl sulfate into deionized water, uniformly dispersing, and stirring to obtain core material liquid; uniformly mixing the core material liquid and the wall material liquid, spraying into a calcium chloride solution, stirring, centrifuging to separate out solid matters, and freeze-drying to obtain a first component;
the method for preparing the second component comprises the steps of adding flammulina velutipes extract, cassia seed extract and oregano extract into deionized water, and uniformly dispersing to prepare an impregnating solution; adding the modified treated matter into the impregnating solution, heating to 40-45 ℃, preserving heat and stirring, filtering out solid matters, and vacuum drying to obtain a second component;
the preparation method comprises the steps of uniformly mixing the first component, the second component, lysozyme, polydextrose and sodium carboxymethylcellulose, and granulating to prepare the feed additive containing probiotics for regulating animal intestinal tracts.
2. The method for preparing a probiotic-containing feed additive for conditioning the intestinal tracts of animals according to claim 1, wherein in the pretreatment, the incubation reaction time after the temperature is raised to 100-110 ℃ is 5-6h;
the volume ratio of the mesoporous activated carbon to the adsorption liquid is 1:2-2.5;
the volume ratio of the adsorption liquid to the pretreatment liquid is 1:0.3-0.4.
3. The method for preparing the probiotic-containing feed additive for conditioning animal intestinal tracts according to claim 1, wherein in the pretreatment, the weight ratio of zinc acetate dihydrate, ferric nitrate nonahydrate, malonic acid and ethanol solution in the adsorption solution is 0.8-1:1.6-2:0.4-0.45:80-90;
the volume concentration of the ethanol solution in the adsorption liquid is 30-35%;
in the pretreatment liquid, the weight ratio of terephthalic acid to N, N-dimethylformamide is 1:50-55.
4. The method for preparing a probiotic-containing feed additive for conditioning animal intestinal tracts according to claim 1, wherein in the modification treatment, the rate of introducing the mixed gas is 1.5-2L/min during the microwave irradiation;
the intermittent microwave heat preservation time is 50-60min;
in the calcination process, the temperature rising rate is 2-3 ℃/min.
5. The method for preparing a probiotic-containing feed additive for conditioning animal intestinal tracts according to claim 1, wherein in the modification treatment, the volume percentage content of oxygen in the mixed gas is 22-23%;
the microwave radiation frequency is 2400-2500MHz, and the microwave power is 1.2-1.3KW.
6. The method for preparing the probiotic-containing feed additive for conditioning animal intestinal tracts according to claim 1, wherein in the compounding, the weight ratio of the modified treatment substance, the light calcium carbonate and the starch is 10-12:8-10:3-4;
the weight ratio of the ball-milling material to the bifidobacterium powder to the lactobacillus plantarum powder to the lactobacillus acidophilus powder to the enterococcus faecalis powder is 80-85:3-4:4-4.5:2-3:1-1.5;
the effective bacterial concentration of the bifidobacterium lactis bacterial powder is more than or equal to 100 hundred million cfu/g;
the effective bacterial concentration of the lactobacillus plantarum bacterial powder is more than or equal to 100 hundred million cfu/g;
the effective bacteria concentration of lactobacillus acidophilus bacteria powder is more than or equal to 100 hundred million cfu/g;
the effective bacterial concentration of the enterococcus faecalis bacterial powder is more than or equal to 1000 hundred million cfu/g.
7. The method for preparing a probiotic-containing feed additive for conditioning the intestinal tracts of animals according to claim 1, wherein in the coating, the volume ratio of the core material liquid to the wall material liquid is 1:1.5-2;
in the wall material liquid, the weight ratio of the sodium alginate to the carboxymethyl cellulose to the deionized water is 1-1.2:0.1-0.15:100-110;
in the core material liquid, the weight ratio of the compound to the glycerol to the sodium dodecyl sulfate to the deionized water is 18-20:4-5:0.2-0.3:100-110;
the concentration of the calcium chloride solution is 0.1-0.15mol/L.
8. The method for preparing a probiotic-containing feed additive for conditioning the intestinal tracts of animals according to claim 1, wherein in the preparation of the second component, the weight ratio of the modified treatment substance to the impregnating solution is 1:5-6;
the heat preservation and stirring time of the modified treatment substance in the impregnating solution is 4-5h;
the weight ratio of flammulina velutipes extract, semen cassiae extract, oregano extract and deionized water in the impregnating solution is 3.5-4:2-2.5:0.8-1:100-110.
9. The method for preparing a probiotic-containing feed additive for conditioning the intestinal tracts of animals according to claim 1, wherein the weight ratio of the first component, the second component, lysozyme, polydextrose and sodium carboxymethyl cellulose in the preparation is 40-50:25-30:0.5-0.6:18-20:0.1-0.15.
10. A probiotic-containing feed additive for conditioning the intestinal tract of animals, characterized in that it is produced by the preparation method according to any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311567032.5A CN117256730B (en) | 2023-11-23 | 2023-11-23 | Probiotic-containing feed additive for conditioning animal intestinal tracts and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311567032.5A CN117256730B (en) | 2023-11-23 | 2023-11-23 | Probiotic-containing feed additive for conditioning animal intestinal tracts and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117256730A true CN117256730A (en) | 2023-12-22 |
| CN117256730B CN117256730B (en) | 2024-04-02 |
Family
ID=89201315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311567032.5A Active CN117256730B (en) | 2023-11-23 | 2023-11-23 | Probiotic-containing feed additive for conditioning animal intestinal tracts and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117256730B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392911A (en) * | 2013-08-06 | 2013-11-20 | 国家粮食局科学研究院 | Feed-use high-activity lactobacillus solid preparation and preparation method thereof |
| KR101465233B1 (en) * | 2014-05-27 | 2014-11-25 | (주)창조바이오텍 | Feed additive for fish comprising Ecklonia cava and Bacillus sp. IS-2 strain |
| US20160038547A1 (en) * | 2014-10-21 | 2016-02-11 | Beijing Kehuitongzhihui Technology., Ltd. | Lactobacillus plantarum capsule for poultry and use thereof |
| CN107594136A (en) * | 2017-09-04 | 2018-01-19 | 安徽秀安生态农业有限公司 | A kind of sorbefacient doll's fish meal |
| CN115707475A (en) * | 2021-08-20 | 2023-02-21 | 江苏省农业科学院 | Core-shell type phage powder and preparation method and application thereof |
| CN116268208A (en) * | 2023-05-17 | 2023-06-23 | 山东广元药业科技有限公司 | Chitosan-containing feed additive for improving animal immunity and preparation method thereof |
| CN116982673A (en) * | 2023-09-27 | 2023-11-03 | 山东广元药业科技有限公司 | Feed additive containing complex enzyme and preparation method thereof |
-
2023
- 2023-11-23 CN CN202311567032.5A patent/CN117256730B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392911A (en) * | 2013-08-06 | 2013-11-20 | 国家粮食局科学研究院 | Feed-use high-activity lactobacillus solid preparation and preparation method thereof |
| KR101465233B1 (en) * | 2014-05-27 | 2014-11-25 | (주)창조바이오텍 | Feed additive for fish comprising Ecklonia cava and Bacillus sp. IS-2 strain |
| US20160038547A1 (en) * | 2014-10-21 | 2016-02-11 | Beijing Kehuitongzhihui Technology., Ltd. | Lactobacillus plantarum capsule for poultry and use thereof |
| CN107594136A (en) * | 2017-09-04 | 2018-01-19 | 安徽秀安生态农业有限公司 | A kind of sorbefacient doll's fish meal |
| CN115707475A (en) * | 2021-08-20 | 2023-02-21 | 江苏省农业科学院 | Core-shell type phage powder and preparation method and application thereof |
| CN116268208A (en) * | 2023-05-17 | 2023-06-23 | 山东广元药业科技有限公司 | Chitosan-containing feed additive for improving animal immunity and preparation method thereof |
| CN116982673A (en) * | 2023-09-27 | 2023-11-03 | 山东广元药业科技有限公司 | Feed additive containing complex enzyme and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| QIAN LUAN等: ""Electrostatically reinforced and sealed nanocellulose-based macrosphere by alginate/chitosan multi-layer coatings for delivery of probiotics"", 《FOOD HYDROCOLLOIDS》, pages 108804 - 108912 * |
| 刘耀东等: ""益生菌微囊化对仔猪生长性能及免疫功能的影响"", 《中国饲料》, pages 55 - 63 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117256730B (en) | 2024-04-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102067941B (en) | Method for producing compound microbial preparation serving as fodder | |
| CN109593666B (en) | Composite microecological preparation and preparation method and application thereof | |
| CN103583880B (en) | Microbial fermentation feed for litopenaeus vannamei | |
| CN101797016A (en) | Animal growth modulating agent and preparation method thereof | |
| CN106922997A (en) | A kind of pig feed additive of efficient gastrointestinal mucosa repair function | |
| CN110946907A (en) | Traditional Chinese medicine composition for young poultry, liquid fermented traditional Chinese medicine preparation and preparation method thereof | |
| CN107970446B (en) | Piglet diarrhea-resistant high-immunity whole egg powder microcapsule and preparation method and application thereof | |
| CN112806498A (en) | Laying hen tea residue bacterium enzyme synergistic fermentation feed and preparation method thereof | |
| CN111513177A (en) | Preparation method of Chinese herbal medicine fermented feed | |
| CN111728098A (en) | Composite probiotics for natural plant fermentation, feed additive for broiler chickens and preparation method thereof | |
| CN114468161A (en) | Fattening pig feed for producing selenium-rich pork and preparation method and feeding method thereof | |
| CN117256730B (en) | Probiotic-containing feed additive for conditioning animal intestinal tracts and preparation method thereof | |
| CN105285327A (en) | Selenium-enriched lactic acid bacterium biological feed additive and preparation method thereof | |
| CN111011629B (en) | Preparation method of antibiotic-free piglet feed | |
| CN108740441A (en) | A kind of functional feed and preparation method that can improve sow feed intake and milk performance | |
| CN111019871A (en) | Preparation method of solid lactobacillus high-activity microbial inoculum for feed | |
| CN113832059B (en) | Antibacterial bacillus and solid fermentation method thereof | |
| CN109497308A (en) | Piglet anti diar rhea additive for microbe feedstuff and preparation method thereof | |
| CN109486713B (en) | Liquid composite lactobacillus preparation and preparation method and application thereof | |
| CN111728081B (en) | Composite bacteria fermentation liquor for feed additive and preparation method thereof | |
| CN102178031A (en) | Process for preparing immune health care material and application method thereof | |
| CN106666151A (en) | Antimicrobial-free additive for preventing and controlling piglet diarrhea and preparation method thereof | |
| CN113615776A (en) | Lactic acid bacteria composite microecological preparation for improving poultry glandular stomach development and application thereof | |
| CN112273551A (en) | Mixed feed additive for preventing piglet diarrhea and application thereof | |
| CN110881579A (en) | Pig intestinal function regulator and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |