CN116983217A - Composition containing phenethyl resorcinol, fennel extract and lactobacillus fermentation product and application thereof - Google Patents
Composition containing phenethyl resorcinol, fennel extract and lactobacillus fermentation product and application thereof Download PDFInfo
- Publication number
- CN116983217A CN116983217A CN202311032750.2A CN202311032750A CN116983217A CN 116983217 A CN116983217 A CN 116983217A CN 202311032750 A CN202311032750 A CN 202311032750A CN 116983217 A CN116983217 A CN 116983217A
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- CN
- China
- Prior art keywords
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- composition
- phenethyl resorcinol
- parts
- fennel extract
- Prior art date
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- 238000000855 fermentation Methods 0.000 title claims abstract description 34
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- A61Q19/08—Anti-ageing preparations
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Abstract
The application belongs to the technical field of daily chemical products, and discloses a composition containing phenethyl resorcinol, a sea fennel extract and lactobacillus fermentation products and application thereof. The composition of the application comprises the following components: phenethyl resorcinol, sea fennel extract and lactobacillus fermentation products. The composition disclosed by the application is prepared by compounding phenethyl resorcinol, a sea fennel extract and a lactobacillus fermentation product, can play a synergistic effect, has multiple effects of resisting oxidation, inhibiting inflammation, whitening, moisturizing and the like, and is mild and free of stimulation.
Description
Technical Field
The application belongs to the technical field of daily chemical products, and particularly relates to a composition containing phenethyl resorcinol, a sea fennel extract and lactobacillus fermentation products and application thereof.
Background
The skin care product with the whitening effect at present mainly achieves the whitening effect by inhibiting tyrosinase activity, inhibiting melanin transfer or accelerating melanin removal. The whitening ingredients that have been developed are mainly divided into two categories: the skin whitening cream is prepared from chemical components such as kojic acid, nicotinamide, glabridin, arbutin and the like, and has obvious whitening effect, but most of the chemical components have the problems of irritation and great side effects, for example, kojic acid has strong tyrosinase inhibition effect and can help to inhibit the generation of color spots and pigments, but has certain biotoxicity, and excessive use of the chemical components can cause the problem of skin white spots. In addition, for the strong-efficacy whitening agent, the problems of redness, swelling and itching occur when part of consumers use the strong-efficacy whitening agent for the first time, so that the strong-efficacy whitening agent has certain limitation in use; the other is plant extracts such as liquorice, green tea, angelica and the like, most of the plant extracts are mild, but the whitening effect is not obvious, the effect is single, and the skin care effect in multiple aspects is difficult to achieve.
Therefore, there is a need to develop a product which is mild and has various skin care effects such as whitening.
Disclosure of Invention
The present application aims to solve at least one of the technical problems in the prior art described above. Therefore, the application provides a composition containing phenethyl resorcinol, sea fennel extract and lactobacillus fermentation product and application thereof, and the composition has multiple effects of resisting oxidation, inhibiting inflammation, whitening, moisturizing and the like, and is mild and has no stimulation.
The first aspect of the present application provides a composition comprising the following components: phenethyl resorcinol, sea fennel extract and lactobacillus fermentation products.
According to some embodiments of the application, the composition comprises the following components in parts by mass: 20-80 parts of phenethyl resorcinol, 10-40 parts of sea fennel extract and 20-60 parts of lactobacillus fermentation product.
According to some embodiments of the application, the composition comprises the following components in parts by mass: 24-63 parts of phenethyl resorcinol, 12-25 parts of sea fennel extract and 25-51 parts of lactobacillus fermentation product.
The second aspect of the application provides a method for preparing the composition of the application, comprising the steps of:
and uniformly mixing the phenethyl resorcinol, the fennel extract and the lactobacillus fermentation product to obtain the composition.
In a third aspect, the application provides the use of a composition according to the application for the preparation of a cosmetic or topical skin preparation.
In a fourth aspect, the present application provides a cosmetic comprising a composition according to the present application.
According to some embodiments of the application, the composition is added to the cosmetic in an amount of 0.1-2.5wt%.
According to some embodiments of the application, the composition is added to the cosmetic in an amount of 0.2-0.5wt%.
According to some embodiments of the application, the cosmetic formulation includes any one of an aqueous agent, an emulsion, a spray, a cream, an essence, or a mask.
In a fifth aspect, the present application provides a facial cream comprising a composition according to the present application and an adjuvant.
According to some embodiments of the application, the adjuvant comprises at least one of a preservative, a thickener, a humectant, an emulsifier, and a skin conditioning agent.
Compared with the prior art, the application has the following beneficial effects:
the composition disclosed by the application is prepared by compounding phenethyl resorcinol, a sea fennel extract and a lactobacillus fermentation product, can play a synergistic effect, has multiple effects of resisting oxidation, inhibiting inflammation, whitening, moisturizing and the like, and is mild and free of stimulation.
Drawings
The application is further described below with reference to the drawings and examples.
FIG. 1 shows the results of the inhibition of NO by different examples and comparative examples;
FIG. 2 shows the result of the inhibition of TNF- α by the different examples and the comparative examples.
Detailed Description
The present application will be described in further detail with reference to specific examples. The starting materials, reagents or apparatus used in the examples are all commercially available from conventional sources or may be obtained by methods known in the art unless otherwise specified. Unless otherwise indicated, assays or testing methods are routine in the art.
In the following examples, the sea fennel (CRITHMUM MARITIMUM) extract and lactobacillus fermentation products were all purchased from the biosciences of tabebuia guangzhou, inc; phenethyl resorcinol is available from deluxe (Shanghai) limited; the solvent 1, 3-propanediol was purchased from Guangdong Dazhixing biotechnology Co.
Example 1
The composition comprises the following components in parts by weight: 25 parts of sea fennel (CRITHMUM MARITIMUM) extract, 51 parts of lactobacillus fermentation product and 24 parts of phenethyl resorcinol.
The preparation method of the composition comprises the following steps: mixing phenethyl resorcinol, sea fennel (CRITHMUM MARITIMUM) extract and lactobacillus fermentation product according to a certain proportion.
Example 2
The composition comprises the following components in parts by weight: 20 parts of sea fennel (CRITHMUM MARITIMUM) extract, 40 parts of lactobacillus fermentation product and 40 parts of phenethyl resorcinol.
The preparation method of the composition comprises the following steps: mixing phenethyl resorcinol, sea fennel (CRITHMUM MARITIMUM) extract and lactobacillus fermentation product according to a certain proportion.
Example 3
The composition comprises the following components in parts by weight: 12 parts of sea fennel (CRITHMUM MARITIMUM) extract, 25 parts of lactobacillus fermentation product and 63 parts of phenethyl resorcinol.
The preparation method of the composition comprises the following steps: mixing phenethyl resorcinol, sea fennel (CRITHMUM MARITIMUM) extract and lactobacillus fermentation product according to a certain proportion.
Comparative example 1
The difference from example 1 is that comparative example 1 uses only 100 parts of a single component, i.e., the extract of sea fennel (CRITHMUM MARITIMUM).
Comparative example 2
The difference from example 1 is that comparative example 2 uses only a single component, namely, 100 parts of lactobacillus fermentation product.
Comparative example 3
The difference from example 1 is that comparative example 3 uses only a single component, namely 100 parts of phenethyl resorcinol.
Comparative example 4
The difference from example 1 is that comparative example 4 uses only two components, namely, 33 parts of sea fennel (CRITHMUM MARITIMUM) extract and 67 parts of lactobacillus fermentation product.
Comparative example 5
The difference from example 1 is that comparative example 5 uses only two components, namely 50 parts of sea fennel (CRITHMUM MARITIMUM) extract and 50 parts of phenethyl resorcinol.
Comparative example 6
The difference from example 1 is that comparative example 6 uses only two components, namely 67 parts of lactobacillus fermentation product and 33 parts of phenethyl resorcinol.
Comparative example 7
The difference from example 1 is that comparative example 7 replaces the sea fennel (CRITHMUM MARITIMUM) extract with the seaweed extract and the other components are used in the same amount as in example 1.
Comparative example 8
The difference from example 1 is that comparative example 8 replaces the lactobacillus fermentation product with the bacillus subtilis fermentation product, and the other components are used in the same amounts as in example 1.
Comparative example 9
The difference from example 1 is that comparative example 9 replaces phenethyl resorcinol with arbutin and the other components are used in the same amount as in example 1.
1. DPPH free radical scavenging experiments
(1) Sample preparation: the samples provided in examples 1-3, comparative examples 1-9 were each dissolved in 30wt%1, 3-propanediol aqueous solution for use.
(2) DPPH free radical scavenging test method
Adding 2.0mL of DPPH test solution with the mass concentration of 45.8mg/L and 0.05mL of 5% sample solution into a test tube, supplementing the total volume to 3mL by 50% ethanol (v/v), shaking uniformly, carrying out light-proof reaction for 30min, and measuring absorbance at 517nm by using A1 cm cuvette, and marking as A1; adding 2mL of absolute ethyl alcohol and a sample to be detected with a corresponding volume into a test tube, supplementing the total volume to 3mL by using 50% ethanol (v/v), and recording the measured absorbance as A2; to the test tube, 2mL of DPPH test solution and 1mL of 50% ethanol (v/v) were added, and the absorbance was measured and designated as A3. Calculating the clearance rate (Y) of the liquid to be tested to DPPH free radicals according to the following formula;
the calculation formula of the DPPH free radical clearance is as follows: y (%) = [1- (A1-A2)/A3 ] ×100%;
wherein: a1 is the absorbance value of the system after the blank is cleared of DPPH, A2 is the absorbance value of the system after the sample is cleared of DPPH, and A3 is the absorbance value of the reaction system before the sample is not added.
(3) IC50 values of each sample were obtained based on DPPH radical scavenging rate, and the results are shown in Table 1.
TABLE 1
As shown in the test results of Table 1, the sea fennel extract, the lactobacillus fermentum and the phenethyl resorcinol all had a certain free radical scavenging effect. As is evident from comparison of examples 1-3 with comparative examples 1-9, the IC50 values of examples 1-3 were smaller than those of comparative examples 1-3 of single component and comparative examples 4-6 of any two component combinations in the case of the same total active ingredient addition amount, indicating that there was a stronger inhibition effect on DPPH when the sea fennel extract, lactobacillus fermentum and phenethyl resorcinol were compounded, and that the improvement of the inhibition effect was not dose-dependent with the active ingredient addition amount, confirming that there was a synergistic effect when the three functional ingredients were compounded. It is assumed that this is because the plant-derived sea fennel extract contains various flavonoid components, the lactobacillus fermentum component contains natural components such as tocopherol and glutathione, and the components can participate in the decomposition of peroxides, while the phenethyl resorcinol is an excellent free radical scavenger, has a strong inhibition effect on oxidation reactions such as chain growth, and the synergistic effect of various components can significantly improve the antioxidation effect.
In addition, comparing the results of example 1 with those of comparative examples 7 to 9, it can be seen that when any component in the composition of the present application is replaced with other similarly effective components, it is difficult to achieve the effect of the composition of the present application. It is presumed that the natural source of the sea fennel extract and the seaweed extract have different contents and types of polyphenols and flavonoids, which affect the scavenging efficiency of free radicals. While different species have a greater impact on the fermentation product, for example: the volatile components in 40 are obtained by fermenting chicory roots by using the North long spore yeast, and only 27 components are obtained by fermenting chicory roots by using the Bacillus pumilus. (Chang Jian, xu Shitao, huang Jing, etc.) analysis of volatile components of chicory root fermentation extracts of different species and application [ J ]. Chinese brewing, 2019, 38 (5): 199-203.) it was therefore presumed that in the composition system of the present application, the product obtained after fermentation with Lactobacillus contains more substances which are advantageous for the synergistic inhibition of free radicals with phenethyl resorcinol, sea fennel extract. As can be seen from comparative examples 1 and 9, the combination of phenethyl resorcinol and sea fennel extract and lactobacillus fermentum has stronger effect on DPPH than arbutin, and the synergistic effect of the three is stronger.
2. Tyrosinase inhibition assay
(1) Sample preparation: the samples provided in examples 1-3, comparative examples 1-9 were each dissolved in 30wt%1, 3-propanediol aqueous solution for use.
(2) Tyrosinase inhibition test method
The sample solution, phosphate Buffer Solution (PBS) with pH value of 6.8 and dopa solution with mass fraction of 0.1% are sucked, fully mixed, incubated at 30 ℃ for 5min, tyrosinase solution (activity 100U/mL) with corresponding volume is respectively added, incubated at 30 ℃ for 10min, quickly transferred into a cuvette, and absorbance is measured at 475 nm.
The relative inhibition of tyrosinase by the sample solution (I) was calculated as follows.
I＝[(A1-A2)-(A3-A4)]/(A1-A2)×100％；
Wherein: a1, A2, A3 and A4 are the absorbance of the solutions of groups 1-4, respectively.
The formulations of the 4 groups of solutions are shown in Table 2.
TABLE 2
| Group of | Group 1 | Group 2 | Group 3 | Group 4 |
| Sample solution (mL) | 0 | 0 | 1.0 | 1.0 |
| Phosphate buffered solution (mL) | 2.0 | 2.5 | 1.0 | 1.5 |
| Dopa solution (mL) | 1.0 | 1.0 | 1.0 | 1.0 |
| Tyrosinase solution (mL) | 0.5 | 0 | 0.5 | 0 |
| Total volume (mL) | 3.5 | 3.5 | 3.5 | 3.5 |
(3) The test results are shown in Table 3.
TABLE 3 Table 3
As can be seen from the test results of Table 3, comparative examples 1 to 3 each use a single sea fennel extract, lactobacillus fermentum and phenethyl resorcinol, and have a certain inhibition effect on tyrosinase, and as can be seen from examples 1 to 3, tyrosinase inhibition effect after combination of sea fennel extract, lactobacillus fermentum and phenethyl resorcinol is significantly higher than comparative examples 1 to 3 and comparative examples 4 to 6 of any two components. The three are compounded with the best effect, which is probably due to the fact that polyphenol groups in the phenethyl resorcinol can be bonded with the active center of tyrosinase, and the phenethyl resorcinol can form hydrogen bonds with the end groups of tyrosinase, so that the catalytic action of tyrosinase on a substrate is reduced. The sea fennel extract contains a certain amount of plant polyphenol, and can occupy relevant sites of enzyme after tyrosinase is combined through end groups, so that the combination of a substrate and the enzyme is inhibited. The lactobacillus ferment contains phytic acid, polyphenol and other components, and has stronger capturing effect on free radicals.
In addition, comparing example 1 with comparative examples 7 to 9, it is understood that the combination of the sea fennel extract, lactobacillus fermentum and phenethyl resorcinol has a stronger tyrosinase inhibitory effect, probably because arbutin is a competitive inhibition of tyrosinase activity, phenethyl resorcinol is a non-competitive inhibition, and the mechanism of action is somewhat different, so that the synergistic effect between the compositions is hindered for replacement of phenethyl resorcinol with arbutin. Furthermore, as mentioned above, the substitution of lactobacillus ferments with bacillus subtilis ferments may also have an effect on the synergistic effect of the composition, as well as the differences in the different plant components and in the different species fermentation components.
3. Inflammatory factor inhibition assay
(1) Sample preparation: the samples provided in examples 1-3, comparative examples 1-9 were each dissolved in 30wt%1, 3-propanediol aqueous solution for use.
(2) Method for testing NO and TNF-alpha inhibition in cells
RAW264.7 cells at 1X 10 4 Cell/well density was added to 96-well plates and placed at 37℃in 5% CO 2 After 24h incubation in incubator, old medium was discarded and washed 2 times with PBS. After 1h of treatment of the cells with the sample solution, complete medium containing 100ng/mL of LPS was added for further incubation for 24h, and corresponding blank (i.e.no LPS and test substance were added) and model (only LPS was added) groups were set, the supernatant was collected, and after centrifugation at 1000rpm for 10min 400. Mu.L of supernatant was taken, and the NO and TNF-. Alpha.content was determined according to the NO detection kit instructions and ELISA kit instructions, respectively. Each set of experiments was independently repeated 3 times.
(3) The results of the inhibition of NO are shown in Table 4, FIG. 1 (average value in Table 4); the results of the inhibition of TNF-. Alpha.are shown in Table 5 and FIG. 2 (average value in Table 5). The average value is that of the inhibition results of 3 groups, namely 3 groups tested in parallel. From the average value, the standard deviation is calculated according to the following formula. The standard deviation can reflect the discrete degree of each data, if the standard deviation is too large, the data is inaccurate, the average value is obtained through a plurality of groups of experiments, and the standard deviation is calculated so as to show whether each group of data has a larger difference from the average value.
Standard deviation:
TABLE 4 Table 4
| Average value of | Standard deviation of | |
| Example 1 | 6.051 | 0.550 |
| Example 2 | 6.418 | 0.379 |
| Example 3 | 6.179 | 0.335 |
| Comparative example 1 | 8.902 | 0.120 |
| Comparative example 2 | 8.056 | 0.193 |
| Comparative example 3 | 8.583 | 0.198 |
| Comparative example 4 | 7.916 | 0.215 |
| Comparative example 5 | 8.504 | 0.243 |
| Comparative example 6 | 7.752 | 0.245 |
| Comparative example 7 | 7.669 | 0.234 |
| Comparative example 8 | 7.255 | 0.255 |
| Comparative example 9 | 7.017 | 0.094 |
| Blank group | 1.413 | 0.113 |
| Model group | 9.356 | 0.267 |
TABLE 5
From the results of tables 4 to 5, the standard deviation of each sample was within a reasonable range. As can be seen from the results of FIGS. 1-2, the sea fennel extract, the lactobacillus fermentum and the phenethyl resorcinol all have a certain inhibitory effect on inflammatory factors. As is evident from comparison of examples 1 to 3 with comparative examples 1 to 9, the compositions of examples 1 to 3 have significantly higher inhibitory effects on inflammatory factors than those of comparative examples 1 to 3 of a single component and comparative examples 4 to 6 of any two component combinations. The results show that the combination effect of the fennel extract, the lactobacillus fermentum and the phenethyl resorcinol is optimal, which is probably due to the fact that the prebiotic components contained in the lactobacillus fermentum in the composition can inhibit inflammatory factors, the fennel extract has a certain inhibiting effect on inflammatory factors, and the polyphenol structure of the phenethyl resorcinol can generate a synergistic effect with small molecular components so as to inhibit the generation of inflammatory factors.
Further, it is understood from comparison of example 1 with comparative examples 7 to 9 that the combination of the sea fennel extract, lactobacillus fermentum and phenethyl resorcinol has a stronger inflammatory factor inhibitory effect, presumably because the different plant components and different species fermentation components, and the different structure and functional group of arbutin and phenethyl resorcinol have an influence on the synergistic effect of the composition.
Application example 1
An essence consists of the following components in percentage by mass: glycerol 8%, 1, 3-butanediol 3%, xanthan gum 0.35%, p-hydroxyacetophenone 0.5%, 1, 2-pentanediol 1.2%, the composition provided in example 1 0.5%, and water in balance.
The preparation method of the essence comprises the following steps:
s1, dissolving the composition provided in the embodiment 1 with 1, 3-butanediol and part of water in advance until the composition is clear and transparent for later use;
s2, dispersing the p-hydroxyacetophenone in advance by using 1, 2-pentanediol, and dissolving the p-hydroxyacetophenone to be transparent at 65 ℃ for standby;
and S3, uniformly dispersing the xanthan gum in the glycerol, adding the residual water, uniformly stirring, adding the standby solution obtained in the steps S1 and S2, and uniformly stirring to obtain the essence.
Comparative example 1 was used
The difference from application example 1 is that the composition provided in comparative example 1 was replaced with the sea fennel (CRITHMUM MARITIMUM) extract provided in comparative example 1, and the other components were used in the same amounts as in application example 1.
Comparative example 2 was used
The difference from application example 1 is that the composition provided in comparative example 2 was used in place of the lactic acid bacterium fermented product provided in comparative example 2, and the other components were used in the same amounts as in application example 1.
Comparative example 3 was used
The difference from application example 1 is that application comparative example 3 replaces the composition provided in example 1 with the phenethyl resorcinol provided in comparative example 3, and the other components are used in the same amounts as in application example 1.
Application example 2
A face cream consists of the following components in percentage by mass: glycerol 4%, 1, 3-butanediol 4%, xanthan gum 0.4%, p-hydroxyacetophenone 0.5%, 1, 2-pentanediol 1.2%, glycerol stearyl alcohol 0.9%, PEG-100 stearate 0.6%, cetostearyl alcohol 0.8%, cetostearyl glucoside 0.2%, behenyl alcohol 3%, isononyl isononanoate 5%, caprylic/capric triglyceride 2%, composition 0.2% provided in example 1, the balance of water.
The preparation method of the face cream comprises the following steps:
s1, dissolving the composition provided in the embodiment 1 with 1, 3-butanediol and part of water in advance until the composition is clear and transparent for later use;
s2, dispersing the p-hydroxyacetophenone in advance by using 1, 2-pentanediol, and dissolving the p-hydroxyacetophenone to be transparent at 65 ℃ for standby;
s3, uniformly dispersing xanthan gum in glycerol, adding residual water, uniformly stirring, adding the standby solution in the step S2, and uniformly stirring to obtain a phase A;
s4, mixing glycerol stearyl alcohol, PEG-100 stearate, cetostearyl alcohol, cetostearyl glucoside, behenyl alcohol, isononyl isononanoate and caprylic/capric triglyceride, and uniformly stirring at 80 ℃ to obtain a phase B;
and S5, heating the phase A in the step S3 to 80 ℃, adding the phase B in the step S4 into the phase A, homogenizing for 3min under the condition of 6000r/min, adding the standby solution in the step S1, and uniformly stirring to obtain the face cream.
Comparative example 4 was used
The difference from application example 2 is that application comparative example 4 replaces the composition provided in example 1 with the composition provided in comparative example 4, and the other components are used in the same amounts as application example 2.
Comparative example 5 was used
The difference from application example 2 is that application comparative example 5 replaces the composition provided in example 1 with the composition provided in comparative example 5, and the other components are used in the same amounts as application example 2.
Comparative example 6 was used
The difference from application example 2 is that application comparative example 6 replaces the composition provided in example 1 with the composition provided in comparative example 6, and the other components are used in the same amounts as application example 2.
4. Sensory evaluation test
198 consumers 20-35 years old are selected for sensory evaluation and testing of the products, and the products are divided into 6 groups of 33 people. The irritation, moisturizing effect and whitening effect of the product containing the composition disclosed by the application are tested, and the specific using method is as follows: after face cleaning, samples of application examples 1-2 and application comparative examples 1-6 to be tested are respectively taken, huang Douli size samples are respectively smeared on the left cheek and the right cheek, the face is massaged for 2 minutes, after 7 days of use, a consumer product use evaluation table is filled in according to sensory evaluation, and the evaluation table is shown in table 6. Wherein the left faces of the 1-3 groups of people all use the sample of application example 1, and the right faces of the 1-3 groups of people use the samples of application comparative examples 1-3, respectively; the samples of application example 2 were used for the left faces of the 4-6 groups of people, and the samples of application comparative examples 4-6 were used for the right faces of the 4-6 groups of people, respectively.
TABLE 6
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As can be seen from table 6, the product added with the composition of the present application can effectively reduce the irritation caused by the high active ingredients, and has good moisturizing and whitening effects, wherein the lactobacillus fermentum and the fennel extract have polyphenol and polysaccharide structures, and the polysaccharide can be combined with cells, has strong hydration, and can play a role in moisturizing. With the improvement of the moisturizing effect, the stratum corneum cell barrier is enhanced, so that the influence of external stimulus on the skin can be well resisted, and the inflammation caused by the strong-efficacy components is reduced, and the effect of reducing the irritation is achieved. Meanwhile, part of polyphenol structures can influence the arrangement of keratinocytes, so that the permeation of functional components is enhanced, and the inhibition effect of the polyphenol components on tyrosinase is combined, so that the synergistic whitening effect can be achieved by compounding of phenethyl resorcinol, sea fennel extract and lactobacillus fermentation products.
5. Skin melanin index MI, skin gloss ITA, and moisture test
A total of 198 male/female subjects with dull facial skin, ranging in age from 18 to 30 years, were selected and divided into 6 groups of 33 persons each. Wherein the left faces of the 1-3 groups of people all use the sample of application example 1, and the right faces of the 1-3 groups of people use the samples of application comparative examples 1-3, respectively; the samples of application example 2 were used for the left faces of the 4-6 groups of people, and the samples of application comparative examples 4-6 were used for the right faces of the 4-6 groups of people, respectively.
1) Skin color change condition
The skin melanin index MI, skin gloss ITA, subjective assessment and taking a picture of the full face of the subject were measured in the stained area of the subject using a front-to-back control method. The evaluation results before and after the use of the product are compared by a statistical test method, and the skin color change condition of the subject is judged.
2) Moisture change condition
Moisture change experiments were tested on consumer's face: skin moisture content test was performed on the left and right nasolabial folds of the volunteer, and the average of 3 values was taken as data (D0) of the nasolabial fold moisture content.
After the initial value test is finished, the volunteer needs to use a test sample according to the specification, the product is required to be smeared for 2 times a day in the morning and in the evening during use, the product is continuously tried for 28 days, the skin color change condition and the skin moisture change condition are recorded, and the test results are shown in Table 7.
TABLE 7
| Sample of | ITA | MI | Moisture change condition |
| Application example 1 | 21.88％ | -22.61％ | 20.90％ |
| Comparative example 1 was used | 10.53％ | -19.68％ | 2.93％ |
| Comparative example 2 was used | 5.13％ | -6.56％ | 8.15％ |
| Comparative example 3 was used | 2.44％ | -6.18％ | 14.98％ |
| Application example 2 | 30.0％ | -25.82％ | 36.00％ |
| Comparative example 4 was used | 21.88％ | -20.88％ | 7.64％％ |
| Comparative example 5 was used | 5.26％ | -8.16％ | 14.72％ |
| Comparative example 6 was used | 2.78％ | -10.52％ | 14.75％ |
As can be seen from Table 7, the products of application examples 1-2 showed more excellent performances in the dimensions of ITA, MI, moisture change, etc., after 28 days of use, as compared with the products of application comparative examples 1-6. In combination with the data (tables 1 and 3) of excellent antioxidant and tyrosinase activity inhibition of the composition of the present application in an in vitro experiment, the products of application examples 1-2 can effectively reduce melanin production by reducing pigmentation caused by free radicals as induction signals, and simultaneously, the composition of the present application has tyrosinase inhibition effect. In addition, the combination of the composition of the application shows inhibition effect on inflammatory factors in an in vitro experiment (table 4 and table 5), and the composition of the application in a product formula can be considered to be capable of repairing damage to skin barrier caused by strong irritation whitening components, inhibiting generation of inflammatory factors, and playing roles of moisturizing, brightening and improving skin color.
While the preferred embodiment of the present application has been described in detail, the application is not limited to the embodiments, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the application, and these modifications and substitutions are intended to be included in the scope of the present application as defined in the appended claims.
Claims (10)
1. A composition, characterized in that it comprises the following components: phenethyl resorcinol, sea fennel extract and lactobacillus fermentation products.
2. The composition according to claim 1, wherein the composition comprises the following components in parts by mass: 20-80 parts of phenethyl resorcinol, 10-40 parts of sea fennel extract and 20-60 parts of lactobacillus fermentation product.
3. The composition according to claim 2, characterized in that it comprises the following components in parts by mass: 24-63 parts of phenethyl resorcinol, 12-25 parts of sea fennel extract and 25-51 parts of lactobacillus fermentation product.
4. A process for the preparation of a composition as claimed in any one of claims 1 to 3, comprising the steps of:
and uniformly mixing the phenethyl resorcinol, the fennel extract and the lactobacillus fermentation product to obtain the composition.
5. Use of a composition according to any one of claims 1 to 3 for the preparation of a cosmetic or topical skin preparation.
6. A cosmetic product, characterized in that it comprises a composition according to any one of claims 1 to 3.
7. The cosmetic according to claim 6, wherein the composition is added to the cosmetic in an amount of 0.1 to 2.5wt%.
8. The cosmetic according to claim 6, wherein the composition is added to the cosmetic in an amount of 0.2 to 0.5wt%.
9. The cosmetic according to claim 6, wherein the formulation of the cosmetic comprises any one of an aqueous agent, an emulsion, a spray, a cream, an essence or a mask.
10. A facial cream comprising the composition of any one of claims 1 to 3 and an adjunct.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| US20220233430A1 (en) * | 2018-06-26 | 2022-07-28 | Symrise Ag | Lactobacillus plantarum for skin care |
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| CN115990129A (en) * | 2022-12-30 | 2023-04-21 | 浙江英树生物科技有限公司 | Sea fennel composition and preparation process and use method thereof |
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| US20110171288A1 (en) * | 2008-02-20 | 2011-07-14 | Fatemeh Mohammadi | Topical compositions and methods for whitening skin |
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