CN112545961A

CN112545961A - Preparation method of whitening and skin-protecting cosmetic

Info

Publication number: CN112545961A
Application number: CN202011585517.3A
Authority: CN
Inventors: 祝冬霞
Original assignee: Ningbo Jiangbei Yirenbao Trade Co ltd
Current assignee: Ningbo Jiangbei Yirenbao Trade Co ltd
Priority date: 2020-12-29
Filing date: 2020-12-29
Publication date: 2021-03-26

Abstract

The invention discloses a preparation method of a whitening skin-care cosmetic, and relates to the technical field of cosmetics. The main functional components in the whitening and skin-care cosmetic prepared by the method are a multifunctional skin-care composition, and the composition specifically comprises the following components: xylitol, oil of radix Salicornia Herbacea, rhizoma Bletillae, herba Hedyotidis Auriculariae, Spirulina platensis, belladonna, and rhizoma et radix Valerianae extract. The composition prepared by the invention is used for cosmetic products, has the main functional components of plant extracts, is natural and safe, has no stimulation, has obvious whitening and moisturizing effects, can help skin to lock moisture, fully improves skin vitality, solves the problem of skin darkness, prevents skin fine lines from generating, and enables the skin to be smooth, glossy and elastic; and can be used for sensitive skin, and has certain barrier repair effect.

Description

Preparation method of whitening and skin-protecting cosmetic

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a preparation method of a whitening and skin-care cosmetic.

Background

With the development of economy and the continuous improvement of living standard, people have higher and higher requirements on beauty and skin care; on the other hand, with the increase of working pressure and environmental pollution, the skin, especially the facial skin, is worse, and the phenomena of dark skin, dry skin, dullness and the like are troubled by more and more people, especially modern women. Whitening and skin care, which are the most important subjects of cosmetics, have been proven by modern scientific studies that the formation of melanin is affected by various factors, and are the result of very complex multi-pathway actions. Most of the currently adopted whitening and beautifying cosmetic additives have the problems of unclear effect and certain adverse reaction.

With the development of plant extraction technology, more and more plant extracts are applied to whitening cosmetics and become effective functional components in the cosmetics. At present, various cosmetics containing plant extracts are available, but the use effect is not satisfactory for various reasons, and therefore, the development of a novel cosmetic having a remarkable whitening and skin-care effect is imperative. How to develop a whitening scheme which is efficient, safe and stable and has synergy becomes a difficult point in the cosmetic industry at present.

Disclosure of Invention

The invention aims to provide a preparation method of a whitening and skin-care cosmetic, which can effectively inhibit the generation of melanin, has good whitening effect, excellent moisturizing effect and good anti-aging performance; has no stimulation to skin and has certain skin barrier repair effect.

The technical scheme adopted by the invention for realizing the purpose is as follows:

a multi-functional skin care composition comprising: xylitol, oil of radix Salicornia Herbacea, rhizoma Bletillae, herba Hedyotidis Auriculariae, Spirulina platensis, belladonna, and rhizoma et radix Valerianae extract. The belladonna and the cowhide of the ear leaf in the composition have synergistic effect with the spirulina platensis, can effectively inhibit the maturation of melanosomes, act on B16 melanocytes, can obviously reduce the formation of the melanocytes, reduce the activity of tyrosinase, inhibit the phagocytosis and transfer of the melanocytes, and enable the skin to be smoother and brighter; the lettuce valerian and the bletilla have synergistic effect, and can obviously improve the preservative effect when being used in cosmetics; the belladonna grass and the valerian lettuce are compounded to effectively absorb ultraviolet rays, remove free radicals, improve the antioxidant activity and prevent aging; belladonna grass and lettuce valine are compounded with xylitol and the oil of the root of the Chinese sage herb, so that the synergistic effect is realized, the moisturizing effect is improved, and the lipid barrier of the skin is repaired; has certain repairing effect on sensitive skin and improves the anti-allergic effect. The composition prepared by the invention is used for cosmetic products, has the main functional components of plant extracts, is natural and safe, has no stimulation, has obvious whitening and moisturizing effects, can help skin to lock moisture, fully improves skin vitality, solves the problem of skin darkness, prevents skin fine lines from generating, and enables the skin to be smooth, glossy and elastic; and can be used for sensitive skin, and has certain barrier repair effect.

Preferably, the multifunctional skin care composition comprises, by weight, 6-8 parts of xylitol, 3-6 parts of Sasa Veitchii root oil and 12-20 parts of an extract.

Preferably, the raw material components of the extract comprise: 10-14 parts of bletilla striata, 9-13 parts of auricularia auricula-judae, 5-8 parts of spirulina platensis, 6-10 parts of belladonna herb and 8-12 parts of valerian lactuca.

Preferably, the preparation method of the extract in the multifunctional skin care composition specifically comprises the following steps: mixing bletilla striata, auricularia auricula-judae roxb, spirulina platensis, belladonna herb and lettuce valerian, drying at 40-45 ℃, and crushing into powder to obtain a mixture, wherein the weight ratio of the materials to the liquid is 1: adding 10-15 g/mL of absolute ethyl alcohol, heating steam at 90-100 ℃, performing reflux extraction for 2h, extracting for 2-3 times, performing vacuum filtration for 2 times, performing rotary evaporation concentration on the filtrate at 50-55 ℃ under the pressure of 0.090-0.1 MPa, and drying at 50-60 ℃ to obtain the extract.

Preferably, the multi-functional skin care composition has a whiteness of > 90%.

The invention also aims to provide application of the multifunctional skin care composition in preparing skin care products, cosmetics and medicines.

Preferably, the skin care product and the cosmetic have the effects of whitening, moisturizing and preventing fine wrinkles.

Preferably, the multifunctional skin care composition further comprises 2-5 parts by weight of barberry. The addition of the barberry extract can reduce melanin under the synergistic effect, enhance the effect of inhibiting tyrosinase activity, improve whitening effect, enhance moisturizing effect and promote skin cell regeneration and cutin renewal; the composition can be used in combination with belladonna herb and lettuce via valine to effectively inhibit inflammatory factors and improve anti-inflammatory ability.

The invention also discloses application of the multifunctional skin care composition in preparing skin care products, cosmetics and medicines.

The invention also aims to provide a whitening and skin-care cosmetic, which comprises the multifunctional skin-care composition.

Preferably, the whitening skin-care cosmetic comprises a cream, paste, ointment, cream, emulsion, oil, mask, gel, liniment, aerosol or solution.

Preferably, the addition amount of the multifunctional skin care composition accounts for 2-8% of the total weight of the whitening skin care cosmetic.

Still another object of the present invention is to provide the use of a whitening skin care cosmetic in sensitive skin.

Preferably, when the whitening skin care cosmetic is prepared, the adopted matrixes, auxiliary materials, carriers, additives and the like are all the prior art, and the preparation method is also mixed and prepared according to a conventional method.

Compared with the prior art, the invention has the following beneficial effects:

in the cosmetic composition prepared by the invention, belladonna grass and valerian lettuce are added, and through compounding or synergistic effect, the formation of melanin can be obviously reduced, the activity of tyrosinase can be reduced, and the skin is smoother and brighter; can effectively remove free radicals, improve the antioxidant activity and prevent aging; the moisturizing effect is obviously improved, the skin barrier repairing effect is achieved, and the anti-allergy property is improved; meanwhile, the composition can be used in cosmetics to effectively improve the anti-corrosion performance of the cosmetics. The addition of the barberry extract plays a role in synergistic enhancement, and the barberry extract is compounded with belladonna herb and lettuce valine for use, so that inflammatory factors are effectively inhibited, and the anti-inflammatory capability is improved. The cosmetic prepared from the composition provided by the invention has the functional components of plant extracts, is natural, safe and non-irritant, has obvious whitening, moisturizing and anti-aging effects, and enables the skin to be smooth, glossy and elastic; can be used for sensitive skin, and has barrier repairing effect on sensitive skin.

Therefore, the invention provides a preparation method of the whitening and skin-care cosmetic, which can effectively inhibit the generation of melanin, has good whitening effect, excellent moisturizing effect and good anti-aging performance; has no stimulation to skin and has certain skin barrier repair effect.

Drawings

FIG. 1 is a graph showing the results of the antiallergic test in test example 2 of the present invention;

FIG. 2 is a comparison of results of the melanin inhibition test in test example 2 of the present invention;

FIG. 3 is a graph showing the comparison of the results of the anti-inflammatory ability test in test example 2 of the present invention;

FIG. 4 is a comparison of results of the corrosion resistance test in test example 3 of the present invention.

Detailed Description

The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:

example 1:

a multifunctional skin care composition comprises xylitol, Salicomia Herbacea root oil, rhizoma Bletillae, herba Hedyotidis Auriculariae, Spirulina platensis, belladonna, and rhizoma et radix Valerianae extract; the raw material components are as follows: 6 parts of xylitol, 3 parts of oil sand grass root oil and 16 parts of extract; wherein, the raw material components of the extract comprise: 10 parts of bletilla striata, 9 parts of auricularia auricula-judae roxb, 5 parts of spirulina platensis, 6 parts of belladonna herb and 8 parts of valerian lactuca.

Wherein, the preparation of the extract:

mixing bletilla striata, auricularia auricula-judae roxb, spirulina platensis, belladonna grass and valerian lactuca according to the weight parts of the formula, drying by electric heating blast at 45 ℃, crushing into powder by using a traditional Chinese medicine crusher to obtain a mixture, and mixing the powder with the powder according to a material-liquid ratio of 1: adding anhydrous ethanol into 15g/mL, heating at 90 deg.C, extracting under reflux for 2h, extracting for 2 times, vacuum filtering for 2 times, rotary evaporating the filtrate at 50 deg.C under 0.090MPa, concentrating, and drying at 60 deg.C to obtain extract.

Example 2:

a multifunctional skin care composition comprises the following raw material components: 8 parts of xylitol, 5 parts of oil sand grass root oil and 14 parts of extract; wherein, the raw material components of the extract comprise: 12 parts of bletilla striata, 10 parts of auricularia auricula-judae roxb, 6 parts of spirulina platensis, 7 parts of belladonna herb and 11 parts of valerian lactuca.

The extract was prepared as in example 1.

Example 3:

a multifunctional skin care composition comprises the following raw material components: 7 parts of xylitol, 6 parts of oil sand grass root oil and 18 parts of extract; wherein, the raw material components of the extract comprise: 13 parts of bletilla striata, 13 parts of auricularia auricula-judae roxb, 8 parts of spirulina platensis, 9 parts of belladonna herb and 10 parts of valerian lactuca.

The extract was prepared as in example 1.

Example 4:

a multifunctional skin care composition comprises the following raw material components: 6 parts of xylitol, 4 parts of oil sand grass root oil and 15 parts of extract; wherein, the raw material components of the extract comprise: 12 parts of bletilla striata, 11 parts of auricularia auricula-judae roxb, 6 parts of spirulina platensis, 8 parts of belladonna herb and 9 parts of valerian lactuca.

The extract was prepared as in example 1.

Example 5:

the difference between the multifunctional skin care composition and the multifunctional skin care composition in the embodiment 1 is that 3 parts by weight of barberry are added into the raw material components.

The extract was prepared as in example 1.

Example 6:

a multi-functional skin care composition was the same as in example 1.

A method for preparing a cosmetic for whitening and caring skin comprises:

s1: adding seaweed glue and EDTA-2Na into water, heating to 80 ℃, and stirring to fully dissolve the seaweed glue and the EDTA-2Na until the seaweed glue is clear and transparent to obtain a solution A;

s2: mixing propylene glycol, nicotinamide, betaine, 1, 2-hexanediol and ethylhexylglycerin to obtain a solution B;

s3: cooling the temperature of the solution A to 60 ℃, adding the solution B, uniformly stirring to completely dissolve the solution B, and keeping the temperature for 30 min;

s4: cooling to 40 deg.C, adding the above multifunctional skin care composition, stirring to dissolve completely, keeping the temperature for 10min, cooling to 25 deg.C, and slowly adding pH regulator to adjust pH to 5.5; and finally adding rose hydrosol, uniformly stirring and standing for 20h to obtain the essence.

Wherein the using amounts of the components in the preparation process are shown in table 1, and the components are calculated by mass percent:

TABLE 1A essence composition

Example 7:

a multi-functional skin care composition was the same as in example 5.

A cosmetic for whitening and caring skin was prepared in the same manner as in example 6.

Comparative example 1:

a multi-functional skin care composition differs from example 1 in that: the raw material components are not added with belladonna grass and lettuce valerian.

Comparative example 2:

a multi-functional skin care composition differs from example 1 in that: belladonna grass is not added in the raw material components.

Comparative example 3:

a multi-functional skin care composition differs from example 1 in that: the raw material components are not added with the lettuce valerian.

Comparative example 4:

a whitening skin-care cosmetic was prepared differently from example 6 in that: the multi-functional skin care composition was prepared as in comparative example 1.

Comparative example 5:

a whitening skin-care cosmetic was prepared differently from example 6 in that: the multi-functional skin care composition was prepared as in comparative example 2.

Comparative example 6:

a whitening skin-care cosmetic was prepared differently from example 6 in that: the multi-functional skin care composition was prepared as in comparative example 3.

Test example 1:

multifunctional composition performance testing

1. Whitening efficacy test

1.1 inhibition of tyrosinase (Biochemical enzymology)

According to each reaction group in Table 2, the reaction solution was precisely transferred to the same 5mL plastic centrifuge tube (EP tube) with cover, mixed uniformly, reacted for 10min at constant temperature (37 ℃) in water bath, 1mL dopa solution was rapidly added, mixed uniformly, reacted for 4min at constant temperature (37 ℃) in water bath, rapidly transferred to a cuvette, and absorbance was measured at 475nm at 5min (PBS solution as blank control) to obtain A_C1、A_C2、A_T1、A_T2. The experiments were performed in parallel for 3 times, and the inhibition rate was calculated according to the following formula:

inhibition rate (%) ([ 1- (T)₁-T₂)/(C₁-C₂)]×100％

TABLE 2 composition of enzyme-inhibiting reaction solution

Reaction group	PBS/mL	Sample solution/mL	tyrosinase/mL
				C₁	1.5	0	0.5
C₂	2	0	0
				T₁	1	0.5	0.5
T₂	1.5	0.5	0

1.2 clearance of DPPH (Oxidation resistance experiment)

According to each reaction group in Table 3, precisely transferring the reaction solution into the same 5mL plastic centrifuge tube (EP tube) with cover, mixing well, standing in the dark for 30min, measuring absorbance at 517nm (ethanol as blank control), to obtain A_c、A_i、A_j. The experiments were performed in parallel for 3 times, and the clearance was calculated according to the following formula:

clearance (%) - (1- (A)_i-A_j)/A_c]×100％

TABLE 3 composition of antioxidant reaction solution

Reaction group	DPPH free radical solution/mL	Sample solution/mL	ethanol/mL
				c	2	0	2
i	2	2	0
				j	0	2	2

Taking the inhibition rate of the sample on tyrosinase and the clearance rate of DPPH free radicals as indexes (the weight is 6:4), and calculating according to the following formula:

whiteness (%) inhibition rate × 0.6+ clearance rate × 0.4

The above test was performed on the compositions prepared in comparative examples 1 to 3 and examples 1 to 5, and the samples were dissolved in absolute ethanol to obtain a 5% mixed solution. The results are shown in Table 4.

Table 4 whitening effect test results

Sample (I)	Tyrosinase inhibition/%)	DPPH clearance/%)	Whiteness/% of
				Comparative example 1	67.3±0.7	74.3±1.1	70.1±0.2
Comparative example 2	68.3±0.9	73.6±0.5	70.4±0.3
				Comparative example 3	89.3±0.4	78.9±0.6	85.2±0.4
Example 1	90.4±0.7	93.6±0.3	91.7±0.2
				Example 2	89.6±0.5	92.2±0.4	90.6±0.5
Example 3	90.1±0.6	92.9±0.7	91.2±0.3
				Example 4	89.9±0.5	92.5±0.3	90.9±0.4
Example 5	94.4±0.3	95.6±0.2	94.9±0.2

As can be seen from the analysis in Table 4, the tyrosinase inhibition rate of the composition prepared in example 1 is obviously higher than that of comparative example 1 and comparative example 2, is equivalent to that of comparative example 3, and is slightly better than that of examples 2-4, which shows that the synergistic effect of the existence of belladonna and the cowhide of the ear leaf and the spirulina platensis can effectively enhance the inhibition effect on the tyrosinase activity; the effect of the composition prepared in the embodiment 1 on removing DPPH is obviously higher than that of comparative examples 1-3 and slightly better than that of embodiments 2-4, and the belladonna and the valerian lactuca are compounded to obviously improve the effect of removing DPPH and improve the oxidation resistance; therefore, the whitening degree of the composition is obviously higher than that of comparative examples 1-3 and slightly better than that of examples 2-4 in the embodiment 1, wherein the comparative example 3 is higher than that of comparative examples 1-2, and the results of the two tests are combined to obtain that the composition is added with belladonna and valerian lactuca, and the belladonna and the valerian lactuca are compounded or synergistically acted with other components, so that the whitening degree of the skin can be effectively improved, and the brightness of the skin can be improved. In addition, the test results of the composition prepared in example 5 are higher than those of example 1, which shows that the addition of the barberry extract plays a role in synergistic enhancement, and further improves the whitening effect.

Test example 2:

1. in vitro anti-allergy test

The inhibition of hyaluronidase activity was determined by the Elson-Morgan modification. In the experimental process, a sample solution with the concentration of 1mg/mL is prepared for later use. 0.1mL of CaCl with the concentration of 0.25mmol/L is taken₂The solution was added with 0.5mL hyaluronidase solution (1250U/mL) and incubated at 37 ℃ for 20min; adding 0.5mL of sample solution, and keeping the temperature at 37 ℃ for 20 min; adding 0.5mL of 0.5g/L sodium hyaluronate solution, keeping the temperature at 37 ℃ for 30min, and then standing at normal temperature for 5 min; then adding 0.1mL of 0.4mol/L NaOH solution and 0.5mL of acetylacetone solution, heating in a boiling water bath for 15min, and immediately cooling with cold water for 5 min; adding 1mL of Ellisib reagent, diluting with 3mL of anhydrous ethanol, mixing, and standing for 20min for color development. And (3) taking dipotassium glycyrrhizinate as a positive control, and measuring a light absorption value by using an ultraviolet visible spectrophotometer. Hyaluronidase inhibition was calculated as follows:

hyaluronidase inhibition rate ═ 1- (C-D)/(a-B) ] × 100%

Wherein A is the light absorption value of a control solution (acetic acid buffer solution is used for replacing a sample solution); b is the light absorption value of the control blank solution (the sample solution and the enzyme solution are replaced by acetic acid buffer solution); c is the light absorption value of the sample solution; d is the absorbance of the sample blank solution (the enzyme solution is replaced by acetic acid buffer solution). The A liquid is scanned in the range of 450 nm-700 nm to determine the maximum absorption wavelength.

The results of the above tests on the compositions prepared in comparative examples 1 to 3 and examples 1 to 4 are shown in FIG. 1. As can be seen from the figure, the hyaluronidase inhibition rate of the composition prepared in the example 1 is 94.1%, which is obviously higher than that of the comparative examples 1-3 and slightly better than that of the examples 2-4, and the existence of belladonna grass and the valerian lactuca is shown, and the two are compounded and then have synergistic effect with xylitol and the oil of the root of the Salicomia europaea, so that the antiallergic performance is effectively improved.

2. Test for Melanin inhibitory Effect

2.1 cell culture

Mouse B-16 melanoma cells were purchased from the Hunan elegant medical college cell Bank, Hunan province. After the cells are fused, they are digested with 0.25% trypsin, subcultured in DMEM high-sugar medium containing 10% calf serum, and placed in CO₂Culturing in an incubator under the culture environment: 37 ℃ and 5% CO₂And (4) saturated humidity. Each experiment was performed on the same passaged cells at an initial inoculum cell concentration of 5000 cells/cm²。

Solution preparation

Firstly, preparing a solution (PEH) containing 300mL/L of absolute ethyl alcohol, 500mL/L of 2-propylene glycol and 200mL/L of double distilled water, then diluting the solution into PEH of 100mL/L by using 0.01M PBS as a solvent, preparing a sample into a concentration of 10g/L by using the PEH before an experiment, and diluting the sample into 0.1g/L for later use.

2.2 cell viability assay

The MTT method is adopted for determination:

the 3 rd generation of mouse B16 melanoma cells were cultured at 6X 10⁴The cells were seeded in 1 96-well plate at a density of 100. mu.L/well, 37 ℃ and 50mL/L CO₂Culturing in an incubator for 1d, removing supernatant, and adding 180 mu L of DMEM high-glucose culture solution and 20 mu L of sample solution into each hole of the experimental group; adding 180 mu of LDMEM high-sugar culture solution and 20 mu of LPHE solution into each hole of the blank control group; blank wells were not seeded with cells; 50mL/L CO at 37 ℃₂Incubating in an incubator for 3d, adding 20 mu L of 0.5 percent MTT into each hole of the experimental group, and continuing incubating for 4 h; the supernatant was then aspirated, 180. mu.L DMSO was added to each well, shaken on a shaker for 10min, the wavelength was selected to be 490nm with an ultraviolet spectrophotometer, and the blank wells were allowed to wither and the absorbance values of each well were measured. The experiment was repeated 4 times. The cell viability inhibition rate was calculated according to the following formula:

cell viability inhibition ═ 1 — (average absorbance value of experimental group/average absorbance value of blank control group) × 100%

2.3 determination of melanin content

Detection was performed using Victoria's method:

the 3 rd generation of mouse B16 melanoma cells were cultured at 1X 10⁴The cells were seeded at a density of 200. mu.L/well in 5 6-well plates at 37 ℃ with 50mL/L CO₂Culturing in an incubator for 1d, removing supernatant, and adding 4.5mL of DMEM high-sugar culture solution and 0.5mL of sample solution into each hole of the experimental group; adding 4.5mL of DMEM high-sugar culture solution and 0.5mL of PHE solution into each well of the blank control group; blank wells were not seeded with cells; 50mL/L CO at 37 ℃₂Incubating in an incubator for 3 days, removing supernatant, adding 1mL of 2.5g/L pancreatin into each hole, digesting at room temperature for 2min, adding 4mL of DMEM high-sugar culture solution to stop digestion, and blowing to obtain single cell suspension; taking 0.5mL single cell suspension, counting cells with cell counting plate, centrifuging the rest suspension in low temperature refrigerated centrifuge at 1500r/min for 10min, discarding supernatant, adding 1mL1M NaOH, shaking for 5min, selecting 490nm wavelength with an ultraviolet spectrophotometer, withering the blank wells, and measuring absorbance values of each well. The experiment was repeated 4 times. The melanin synthesis inhibition rate was calculated according to the following formula:

melanin synthesis inhibition rate ═ 1- (experimental well average absorbance value/experimental well average cell density)/(blank control well average absorbance value/blank control well average cell density) ] × 100%

The results of the above tests on the compositions prepared in comparative examples 1 to 3 and examples 1 to 5 are shown in FIG. 2. From the analysis of the figure, the cell viability inhibition rate and the melanin inhibition rate of the composition prepared in the example 1 are 66.8% and 81.3% respectively, are obviously higher than those of the comparative example 1 and the comparative example 2, are equivalent to those of the comparative example 3, and are slightly better than those of the examples 2-4, so that the inhibition effect on the cell viability can be effectively improved due to the synergistic effect of the belladonna and the spirulina platensis, and the melanin generation inhibition effect is remarkably improved. Example 5 is more effective than example 1, indicating that the presence of barberry acts as a synergistic enhancement.

3. Anti-inflammatory Capacity test

The anti-inflammatory capacity of the composition was determined using the RAW264.7 macrophage respiratory burst test. Preparation of cell concentration 2X 10⁶one/mL of RAW264.7 cell suspension is ready for use. Firstly, preparing a solution (PEH) containing 300mL/L of absolute ethyl alcohol, 500mL/L of 2-propylene glycol and 200mL/L of double distilled water, then diluting the solution into PEH of 100mL/L by using 0.01M PBS as a solvent, and preparing a sample into a concentration of 10g/L by using the PEH and diluting the sample into 0.06g/L before an experiment. 250 mu L of cell fluid, 10 mu L of sample fluid with different concentrations and 60 mu L of luminol luminous solution diluted by 10 times by DMEM are sequentially added into a 96-well plate, and 2.5 mu L of stimulant PMA with the concentration of 0.02mg/mL is added after uniform mixing. Reacting at 37 deg.C for 40min, setting chemiluminescence apparatus to record experimental data every 1min, and measuring peak value A₁The peak value A was obtained by substituting ultrapure water for the sample₀Each set of samples was repeated 3 times and averaged. The inhibition rate calculation formula is as follows:

inhibition ratio (%) ═ a₀-A₁)/A₀×100％

The results of the above tests on the compositions prepared in comparative examples 1 to 3, example 1 and example 5 are shown in FIG. 3. Analysis in the figure shows that the combined inflammatory reaction inhibition rate of the sample prepared in the example 1 is only slightly higher than that of the sample prepared in the comparative example 1, and the inflammatory inhibition effect of the sample prepared in the example 5 is obviously better than that of the sample prepared in the example 1, so that the combination of the barberry flower, the belladonna herb and the valerian lettuce can effectively inhibit inflammatory factors and improve the anti-inflammatory capability.

Test example 3:

1. safety test

Selecting 30 healthy female volunteers without skin diseases, wherein the female volunteers are 20-35 years old; the test method comprises the following steps: examples 6 to 7 were subjected to patch test. Selecting a qualified spot tester, placing about 0.020-0.025 mL of a tested object into the spot tester by using a closed spot test method, externally applying a medical adhesive tape to the back of the tested object, removing the tested object after 24 hours, observing skin reactions 0.5, 24 and 48 hours after the spot is removed, and recording the results according to the skin reaction grading standard in technical Specification for cosmetic safety (2015 edition).

TABLE 5 grading Standard of adverse skin reactions

The test result shows that the test result of the human skin closed patch shows that 0 of 30 persons has positive reaction, and the test object does not cause adverse skin reaction to the batch of testees according to the regulation in technical Specification for cosmetic safety (2015 edition).

2. Test of Corrosion resistance

With reference to the recommended method for PCPC in the United states, 100g of the sample was weighed and mixed with the bacterial and fungal suspensions to give a product with 10 bacterial and fungal contents, respectively⁶And 10⁴CFU/g. Mixing well, and storing at 28 deg.C. After inoculation, samples were taken at 0d, 28d for analysis: accurately weighing 10g of sample, adding 90g of sterilized physiological saltMixing with water, standing at room temperature for 15min to obtain 1:10 diluted solution, sequentially diluting with sterilized normal saline until the dilution degree is 10^-7. Adding 1.0mL of each gradient diluent into a sterilized culture dish containing 15mL of culture medium, fully shaking, standing at room temperature for 20min, culturing bacteria at 37 +/-1 ℃ for 24h and culturing fungi at 28 +/-1 ℃ for 48h, repeating the dilution gradient for 3 times, observing experimental phenomena, and recording the number of colonies.

The results of the above tests on comparative examples 4 to 6, example 6 and example 7 are shown in FIG. 4. Analysis shows that the bacterial colony number and the fungal colony number of the essence prepared in the example 6 are respectively 42 and 21, which are obviously less than those of the comparative examples 1-3, and the existence of the valerian lactuca and the bletilla striata are shown to have a synergistic effect, and the preservative property of the essence can be obviously enhanced by adding the valerian lactuca and the bletilla striata into the essence. The effect of example 7 was better than that of example 6, indicating that the addition of barberry had a synergistically enhanced effect.

3. Evaluation of moisture Retention Property

25 healthy female volunteers without skin diseases were selected and tested by a CM825 model skin moisture tester at an ambient temperature of (22. + -.2) ° C and a relative humidity of (50. + -.5)%. Before the start of the experiment, each volunteer washed his arms and dried, and a 4cm × 4cm area was drawn on the forearm of each volunteer as a test area, with the interval between the areas being 1 cm. The volunteers were then left to stabilize their forearms for 30min in the test environment, during which time the mood was kept calm and no water or smoke was allowed to drink. After stabilization, the coating amount is (2 +/-0.2) mg/cm²The sample to be tested is uniformly smeared on the tested area by using a latex finger stall. Measuring the moisture content of the skin before sample application and 1h, 3h and 5h after sample application, testing each area for 6 times, averaging, and calculating the moisture change rate of the skin. The skin moisture content testing procedure was performed by the same experimenter. The skin moisture change rate was calculated as follows:

skin moisture change rate (%) - (W)_i-W₀)/W₀

In the formula, W_iRepresents the measurement value after the i hour after the sample is smeared; w₀The measurement value before smearing the sample is shown.

The results of the above tests on the essences prepared in comparative examples 4 to 6 and examples 6 to 7 are shown in table 6.

TABLE 6 measurement results of skin moisture content

The results of analysis in the table 6 show that the moistening and moisturizing effects of the essence prepared in the example 6 are obviously higher than those of the comparative examples 4-6, and the belladonna herb and the valerian lactuca exist, and are compounded to be synergistic with the xylitol and the oil of the root of the Salicomia europaea, so that the moisturizing performance of the essence can be effectively improved by adding the essence, and the long-acting moisturizing effect is achieved. The effect of example 7 was better than that of example 6, indicating that the presence of barberry had some enhancing effect.

4. Water Loss (TEWL) determination

The skin moisture loss TEWL is an important parameter for evaluating the function of a skin moisture protective layer and can be used for evaluating the strength of the skin barrier function. If the skin barrier function is impaired, TEWL will increase, and the skin will become dry, desquamated, sensitive, etc. The higher the moisture content and the lower the value of the skin moisture loss TEWL if the skin protection layer is better. The test method comprises the following steps: 50 volunteers of 20-35 years old are selected as trial objects, the trial objects are randomly divided into 5 groups (10 in each group), and the skin of the test subject is healthy and meets the selection standard. After the essence samples of the equal amount of the examples 6-7 and the comparative examples 4-6 are smeared on the skin on the inner side of the arm, the amounts of the water loss of the skin at the 1 st day and the 30 th day after the essence is used are respectively tested by using a Tewameter TM300, the test is repeated for 5 times respectively, an average value is obtained, the water loss change is reflected in a test period, and the change rule of the water loss of a test area along with the time is obtained. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site.

Rate of change (water loss after use-initial water loss)/initial water loss

The test results are shown in table 7.

TABLE 7 skin TEWL assay results

From the table 7, the essence prepared in the example 6 after 30 days of use enables the change rate of the skin TEWL value to reach more than 50%, which is obviously higher than that of the essence prepared in the examples 4-6, and shows that the belladonna herb and the valerian lactuca are compounded and then have a synergistic effect with the xylitol and the oil of the root of the Salicomia europaea, and after the essence is added, the barrier function of the skin can be enhanced, so that a good moisturizing effect is achieved, and the skin moisturizing effect is consistent with the moisturizing effect test result. The effect of example 7 was better than that of example 6, indicating that the presence of barberry had some enhancing effect.

Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims

1. A multi-functional skin care composition comprising: xylitol, oil of radix Salicornia Herbacea, rhizoma Bletillae, herba Hedyotidis Auriculariae, Spirulina platensis, belladonna, and rhizoma et radix Valerianae extract.

2. The multifunctional skin care composition of claim 1, wherein: the multifunctional skin care composition comprises, by weight, 6-8 parts of xylitol, 3-6 parts of Salicomia Herbacea root oil and 12-20 parts of an extract.

3. The multifunctional skin care composition of claim 1, wherein: the preparation method of the extract in the multifunctional skin care composition specifically comprises the following steps: mixing bletilla striata, auricularia auricula-judae roxb, spirulina platensis, belladonna herb and lettuce valerian, drying at 40-45 ℃, and crushing into powder to obtain a mixture, wherein the weight ratio of the materials to the liquid is 1: adding 10-15 g/mL of absolute ethyl alcohol, heating steam at 90-100 ℃, performing reflux extraction for 2h, extracting for 2-3 times, performing vacuum filtration for 2 times, performing rotary evaporation concentration on the filtrate at 50-55 ℃ under the pressure of 0.090-0.1 MPa, and drying at 50-60 ℃ to obtain the extract.

4. The multifunctional skin care composition of claim 1, wherein: the whitening degree of the multifunctional skin care composition is more than 90%.

5. Use of the multifunctional skin care composition according to any one of claims 1 to 4 for the preparation of skin care products, cosmetics and pharmaceuticals.

6. Use of a multifunctional skin care composition according to claim 5, characterized in that: the skin care product and cosmetic have effects of whitening skin, keeping moisture, and preventing fine lines.

7. A whitening skin care cosmetic comprising the multifunctional skin care composition of claim 1.

8. The whitening and skin-care cosmetic as set forth in claim 7, wherein: the whitening and skin-protecting cosmetic is cream, ointment, emulsion, oil agent, gel or aerosol.

9. The whitening and skin-care cosmetic as set forth in claim 7, wherein: the addition amount of the multifunctional skin care composition accounts for 2-8% of the total weight of the whitening skin care cosmetic.

10. Use of a whitening skin care cosmetic of claim 7 for sensitive skin.