CN116918583A - Device and method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches - Google Patents
Device and method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches Download PDFInfo
- Publication number
- CN116918583A CN116918583A CN202310723659.9A CN202310723659A CN116918583A CN 116918583 A CN116918583 A CN 116918583A CN 202310723659 A CN202310723659 A CN 202310723659A CN 116918583 A CN116918583 A CN 116918583A
- Authority
- CN
- China
- Prior art keywords
- carbon dioxide
- box body
- branches
- box
- tea tree
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000003553 Leptospermum scoparium Species 0.000 title claims abstract description 62
- 235000009024 Ceanothus sanguineus Nutrition 0.000 title claims abstract description 61
- 235000015459 Lycium barbarum Nutrition 0.000 title claims abstract description 61
- 230000004083 survival effect Effects 0.000 title claims abstract description 30
- 238000000338 in vitro Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title abstract description 10
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 180
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 96
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 96
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 84
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 18
- 239000001301 oxygen Substances 0.000 claims abstract description 18
- 238000005520 cutting process Methods 0.000 claims description 41
- 235000016709 nutrition Nutrition 0.000 claims description 34
- 230000035764 nutrition Effects 0.000 claims description 33
- 239000000126 substance Substances 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 5
- 238000002309 gasification Methods 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 4
- 239000000779 smoke Substances 0.000 claims description 4
- 239000006199 nebulizer Substances 0.000 claims 5
- 230000005540 biological transmission Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 239000004744 fabric Substances 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
- 239000003595 mist Substances 0.000 abstract description 18
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract description 11
- 238000004321 preservation Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 18
- 238000003860 storage Methods 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 241001122767 Theaceae Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 235000013616 tea Nutrition 0.000 description 10
- 239000002245 particle Substances 0.000 description 7
- 239000007789 gas Substances 0.000 description 5
- 238000005507 spraying Methods 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000241 respiratory effect Effects 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000009569 green tea Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 150000004053 quinones Chemical class 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 208000031704 Autosomal spastic paraplegia type 30 Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 101000971638 Homo sapiens Kinesin-like protein KIF1A Proteins 0.000 description 1
- 102100021527 Kinesin-like protein KIF1A Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 201000007766 hereditary spastic paraplegia 30 Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004202 respiratory function Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/02—Treatment of plants with carbon dioxide
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a device and a method for prolonging the in-vitro survival time of tea tree germplasm resource cuttage branches, and relates to the technical field of germplasm resource in-vitro preservation. The device for prolonging the in-vitro survival time of the tea tree germplasm resource cuttage branches comprises an electric sprayer, a cover, a box body and a carbon dioxide module; the electric sprayer is arranged above the cover, the cover covers the upper end of the box body, and the carbon dioxide module is arranged at the bottom of the box body; the electric sprayer is a USB miniature water pump; the box body comprises a carbon dioxide chamber, a water tank and a planting plate; the carbon dioxide module is arranged at the bottom of the box body and comprises a carbon dioxide accommodating chamber, a control system box and a carbon dioxide sensor. According to the invention, carbon dioxide is introduced into the box body, so that the oxygen content is reduced, the respiration of branches is inhibited, and the consumption of organic matters of the branches is reduced; the water mist sprayed into the box body not only plays a role in preserving, but also gasifies the water mist to take away heat, so that the temperature in the box body is reduced.
Description
Technical Field
The invention relates to the technical field of in-vitro preservation of germplasm resources, in particular to a device and a method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches.
Background
Cutting is also called cutting, is a common propagation method for cultivating plants, the cutting belongs to asexual propagation, the root system of cutting seedlings is formed by varieties per se, new individuals can fully retain the characters of parents, and the method is suitable for rapid propagation of varieties. The plant can be cut to obtain stems, leaves, roots, buds and the like, and the plant can be inserted into soil, sand or soaked in water, and can be planted after rooting, so that the plant becomes an independent new plant, and the plant can be divided into three types of stem cutting, root cutting and leaf cutting according to different cutting organs.
Tea tree is of the family Theaceae, the genus Thea is evergreen shrub or small arbor, the tender branch is hairless, and is usually in the shape of cluster shrubs. She Bao, elliptic needle shape or oblong shape, length of 4-12 cm, width of 2-5 cm, blunt or sharp tip, wedge-shaped base, shiny upper surface, no hair below or soft hair at initial time, 5-7 pairs of side veins, sawtooth at edge, 3-8 mm long petiole, no hair; flowers become umbrella inflorescences, white and pedicel is bent downwards; capsule spherical shape; the seeds were tan. Flowering period is 9-10 months, and fruit period is 11 months. Tea trees are distributed all over the world, and the growth needs proper sunlight, temperature and water, and needs thicker soil layer, low groundwater level, higher soil fertility, moderate shading and warmth and cold resistance. The tea tree can be prepared into tea leaves and seeds by picking tender leaves in spring and autumn, can squeeze oil and has fine trunk materials, can be used for carving, is rich in various nutritional ingredients, has special medical care effects, and has good prevention effects on radiation, cancer, anemia and other diseases besides reducing cholesterol and preventing atherosclerosis. People in China find and utilize tea trees at the earliest time, and have the most tea varieties in the world, and the tea carrying technology, the tea making technology, the tea drinking habit and the like in all parts of the world are all derived from China. In recent years, on one hand, along with the cognition of consumers on the health care efficacy of tea, the demand is gradually increased, and the planting area of tea trees is continuously increased. The tea tree germplasm resource is the material basis for production and utilization, variety innovation and biotechnology research and is also the potential for the sustainable development of the tea industry.
Germplasm resources, also called variety resources, genetic resources or genetic resources, are taken as important components of biological resources, are the material basis for cultivating new varieties of crops with high quality, high yield, disease resistance and stress resistance, are strategic resources for human society survival and development, and are important guarantees for improving the comprehensive production capacity of agriculture and maintaining the food safety of China; is an important foundation for the sustainable development of national strategic resources and Chinese agriculture, a basic resource for productivity, a material foundation for species evolution, genetic research and plant breeding and a core material for bioengineering technological innovation. The germplasm resource not only provides raw materials for clothing, food and the like of human beings, provides nutrition and medicines for the health of the human beings, but also provides good environment for the happy survival of the human beings, and simultaneously provides inexhaustible gene sources for breeding new varieties and developing biotechnology researches. The protection, research and utilization of good germplasm resources are the requirements of national agricultural technological innovation and national force enhancement, are the requirements of competing international markets, and meanwhile, the conservation of plant germplasm resources has become a focus of global attention, so that the reasonable utilization of germplasm resources enables breeding to achieve a lot of remarkable achievement.
After the tea tree germplasm resources are collected in different places, due to the fact that the transportation distance is long, the storage time is long, the utilization rate and the survival rate of tea tree branches are low due to the fact that the branches lose water and other factors, not only are tea tree germplasm resources wasted and economic losses caused, but also rare tea tree species cannot be promoted in a large area. How to effectively prolong the in vitro survival time of tea tree seed resources, effectively preserve excellent seed resources, and realize the sustainable development of the tea industry to be a technical problem which needs to be solved by the technicians in the field.
The device for prolonging the in-vitro survival time of the tea tree germplasm resource cuttage branches in the prior art has the following problems:
1. at present, after the cutting branches of the tea trees are separated from the parent of the tea trees, the cutting branches are usually placed in a common carton, the branches are often stacked together, the branches often consume more organic matters per se due to overhigh temperature, and the survival rate after cutting is reduced.
2. The branches are placed in a common paper box, the oxygen content in the box is high, and the branches consume more self-stored organic matters through self respiration.
3. The nutrition needed by the branches is difficult to be placed in the common paper box, and under the conditions of long-distance transportation, long storage time and the like, the branches only consume the nutrition stored by themselves, so that the survival rate is reduced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a device and a method for prolonging the in-vitro survival time of tea tree germplasm resource cuttage branches, so as to solve the problems in the prior art, the cuttage branches of tea trees are separated from parent bodies of the tea trees and then are immediately subjected to fresh-keeping treatment, the treated cuttage branches of the tea trees are placed on a planting plate in the device, and small holes are distributed on the planting plate, so that the cuttage branches are vertically placed, and mutual extrusion among the branches is avoided; the bottom of the device is provided with a nutrition chamber for containing nutrition liquid needed by branches; the bottom of the device is provided with the carbon dioxide storage chamber which is communicated with the inside of the device, and the carbon dioxide is discharged into the device to reduce the oxygen content in the device, reduce the respiration of branches and reduce the consumption of organic matters; the electric spraying device is designed on the top cover, the water tank is designed in the middle of the device, the electric spraying device sucks water in the water tank, the water is sprayed into small-particle mist through the nozzle, the mist takes away heat in the device, the internal temperature is reduced, the activity of respiratory enzymes can be affected when the temperature is reduced, the self-respiration of the tea tree cutting branches is inhibited, the consumption of organic matters is reduced, and the survival rate of the cutting branches is increased.
In order to achieve the technical purpose and the technical effect, the invention is realized by the following technical scheme:
a device for prolonging the in-vitro survival time of tea tree germplasm resource cuttage branches comprises an electric sprayer, a cover, a box body and a carbon dioxide module; the electric sprayer is arranged above the cover, the cover covers the upper end of the box body, and the carbon dioxide module is arranged at the bottom of the box body; the electric sprayer is a USB miniature water pump and comprises an enlarged 365 motor, a water pump, four nozzles, a USB wiring and a water pipe; five small round holes are formed in the cover for the water pipe of the electric sprayer to penetrate through the upper cover and extend into the box body; the bottom of the box body is provided with a nutrition chamber, the planting plate is placed above the nutrition chamber, the middle of the box body is provided with a water tank, and the middle of the water tank is provided with a carbon dioxide sensor mounting groove; the carbon dioxide module is arranged at the bottom of the box body and comprises a carbon dioxide accommodating chamber, a control system box and a carbon dioxide sensor.
Further, the electric atomizer model is a USB miniature suction pump, comprises increase 365 motors, water pump, four nozzles, USB wiring, water suction pipe, switch etc. electric atomizer can use through the cooperation of USB wiring and mobile power supply such as treasured that charges, press shift knob, increase 365 motors access power after, the motor operation, the drive the water pump utilizes water in the water tank is absorbed to the water suction pipe, later passes through the nozzle is erupted water mist of granule into the box, takes away the heat when the water mist gasification, reaches the purpose of reducing the incasement temperature.
Further, the cover is arranged on the upper end face of the box body, and 5 small round holes are formed in the cover and used for allowing the water suction pipe and the nozzle on the electric sprayer to pass through and enter the box body.
Further, a thermometer groove is formed in the edge of the top of the box body, a thermometer is placed in the thermometer groove, and the temperature in the box body is detected in real time; a nutrition room is designed in the box body, and nutrition substances required by the growth of the branches are poured into the nutrition room for nutrition required by the survival of the branches; the planting plate is arranged at the upper end of the nutrition room, a plurality of small round holes are formed in the planting plate, the tea tree cutting branches are placed in the small round holes, the branches are ensured to be placed vertically, the branches are prevented from being extruded mutually, and each branch is ensured to absorb nutrition substances stored at the bottom; a carbon dioxide air inlet is formed in the center of the inside of the box body, carbon dioxide enters the box body through the air inlet, part of air in the box body is discharged out of the box body through the gas one-way valve, and the oxygen content in the box body is reduced; the gas one-way valve is designed on the outer surface of the top of the box body; the top of the carbon dioxide is provided with a water tank for containing preservative, a water suction pipe of the electric sprayer sucks the liquid stored in the water tank into a water pump, and the liquid is sprayed to all parts of the branches by a nozzle; the middle position of the water tank is provided with a carbon dioxide sensor mounting groove, the model of the carbon dioxide sensor is SGP30, the carbon dioxide sensor is mounted in the water tank, the carbon dioxide sensor detects the concentration of carbon dioxide in the water tank, a detected signal is transmitted into a control system box, and the control system box opens or closes an air pipe switch according to the concentration in the water tank.
Further, the carbon dioxide module comprises a carbon dioxide storage chamber, a control system box and a carbon dioxide sensor, wherein the carbon dioxide sensor is arranged at the middle part of the water tank, detects the concentration of carbon dioxide in the box body, transmits detected signals into the control system box, and controls the opening or closing of the carbon dioxide storage chamber according to the received signals by the box body control box, so that the oxygen environment in the box body is changed.
Further, another object of the present invention is to provide a method for using a device for prolonging the in vitro survival time of the cuttage branches of tea tree germplasm resources;
opening an upper cover of the device for prolonging the in-vitro survival time of the cuttage branches of the tea tree germplasm resources, taking out the field planting plate, adding nutrient substances required by in-vitro culture of the cuttage branches into a nutrition chamber, and then installing the field planting plate at the upper end of the nutrition chamber.
Further, after the tea tree cutting branches are separated from the tea tree parent, the branches are immediately subjected to fresh-keeping treatment, and immediately after the fresh-keeping treatment, the branches are placed in small round holes in the planting plate, and then the cover is covered.
Further, after the cover is covered, the carbon dioxide control system box is started, carbon dioxide is introduced into the box body, partial air in the box body is discharged, the oxygen content in the box body is reduced, and the respiration of branches is inhibited.
Further, touching the switch of the electric sprayer starts the electric sprayer, liquid is sucked from the water tank, the liquid is sprayed into small-particle water mist through the nozzle to enter the tank, and the small-particle liquid gasifies to take away heat in the tank.
The invention has the following beneficial effects:
after the tea tree cutting branches are separated from the tea tree parent, the fresh-keeping treatment of the branches is immediately carried out, so that the fresh-keeping time of the tea tree branches is prolonged, the activity of the branches is kept, the toxic action of harmful microorganisms such as bacteria on the tea tree branches is reduced, and the loss of nutrient substances of the tea tree branches is reduced;
placing the treated tea tree cutting branches on a planting plate in the device, wherein small round holes are fully distributed on the planting plate, and the cutting branches are vertically placed in the small round holes, so that mutual extrusion among the branches is avoided, and the branches are ensured to pass through the small round holes to absorb nutrient substances;
the bottom inside the device is provided with a nutrition chamber for containing nutrition liquid needed by branches and supplying nutrition needed by normal life activities of tea branches; the bottom of the device is provided with the carbon dioxide storage chamber, and carbon dioxide is discharged into the device to reduce the oxygen content in the device, reduce the respiration of the branches and reduce the consumption of organic matters of the branches;
the electric spraying device is designed on the top cover, the water tank is designed in the middle of the device, the electric spraying device absorbs liquid in the water tank, the water is sprayed into small-particle water mist through the nozzle, the freshness of the branches is enhanced, meanwhile, the heat in the device is taken away by gasifying the water mist, the internal temperature is reduced, the activity of respiratory enzymes is affected by the reduction of the temperature, the respiration of the tea tree cutting branches is inhibited, the consumption of organic matters of the branches is reduced, and the survival rate of the cutting branches is increased.
Drawings
FIG. 1 is an overall elevation of an apparatus according to an embodiment of the invention;
FIG. 2 is a plan view of the whole device according to the embodiment of the invention;
FIG. 3 is a plan view of a device housing according to an embodiment of the present invention;
FIG. 4 shows an electric sprayer of the device according to an embodiment of the invention;
FIG. 5 is a schematic illustration of a field plate according to an embodiment of the present invention;
fig. 6 is a schematic diagram of a carbon dioxide module according to an embodiment of the invention.
In the drawings, the reference numerals are as follows:
1-electric sprayer switch, 2-electric sprayer, 3-nozzle, 4-lid, 5-thermometer chamber, 6-box, 7-planting board, 8-nutrition room, 9-carbon dioxide room, 10-carbon dioxide intake pipe, 11-water tank, 12-water pipe, 13-carbon dioxide sensor, 14-carbon dioxide module, 15-power supply bin, 16-gas check valve, 701-aperture, 702-centre hole, 1401-carbon dioxide apotheca, 1402-control system box, 1403-carbon dioxide intake pipe.
Detailed Description
In order to more clearly describe the technical scheme of the embodiment of the present invention, the embodiment of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
Fig. 4 shows an electric sprayer according to an embodiment of the present invention, and as shown in fig. 4, the electric sprayer is a USB micro-pump, which includes an enlarged 365 motor, a pump, four nozzles, a USB connection, a suction pipe 12, a switch 1, and the like.
In this embodiment, electric atomizer can pass through USB wiring and mobile power supply cooperation such as treasured that charges use, push down shift knob 1, increase 365 motor circular telegram back, increase 365 motor operation, drive electric atomizer water pump utilizes the water suction pipe absorbs 11 liquid in the water tank, later pass through the nozzle erupts water spray into the mist of tiny particle, and the mist sprays on the branch, except playing fresh-keeping effect, can consume the heat in the box when the mist gasification, reaches the purpose that reduces the incasement temperature.
In this embodiment, a mobile power source may be placed in the power source compartment 15 on the cover to provide power for driving the electric sprayer.
Example 2
Fig. 1-2 are overall schematic diagrams of an apparatus for prolonging survival time of tea tree germplasm resource cutting branches in vitro according to an embodiment of the present invention, as shown in fig. 1-2, the apparatus for prolonging survival time of tea tree germplasm resource cutting branches in vitro comprises an electric sprayer, a cover, a box body, and a carbon dioxide module, wherein the cover 4 is designed with five small round holes, so that a water suction pipe 12 and a spray gun 3 of the electric sprayer penetrate through the cover 4 and enter the box body; fig. 3 is a box body according to an embodiment of the present invention, as shown in fig. 3, a carbon dioxide chamber 9 is designed at the bottom of the box body 6, a nutrition chamber 8 is designed at the bottom of the inner surface of the box body, the planting plate 7 is installed on the nutrition chamber 8, a carbon dioxide air inlet pipe 10 and a water tank 11 are designed at the center of the box body, and the carbon dioxide sensor 13 is installed in the middle of the water tank 11.
In this embodiment, a carbon dioxide chamber is designed at the bottom of the box body 6, the carbon dioxide module 14 is placed therein, the type of the carbon dioxide sensor adopted by the invention is SGP30, the carbon dioxide module 14 comprises a carbon dioxide storage chamber 1401, a control system box 1402, a carbon dioxide air inlet pipe 1403 and a carbon dioxide sensor 13, the carbon dioxide sensor 13 detects the concentration of carbon dioxide in the box body 6, the detected signal is transmitted to the control system box 1402, the control system box controls the carbon dioxide storage chamber to be opened or closed after receiving the fed back signal, thereby controlling the carbon dioxide amount introduced into the box body, carbon dioxide enters the box body through the carbon dioxide air inlet pipe 10, the density of the carbon dioxide is higher than that of the air, part of the air in the box body is introduced into the carbon dioxide by the bottom of the box body, the air in the box body is discharged outside through a gas one-way valve on the outer surface of the box body, the oxygen content in the box body is reduced, the respiration of the branches is further suppressed, and the consumption of nutrition substances of the branches themselves by cutting are reduced.
In this example, tea tree is rich in phenols, wherein ester catechin is the main component of green tea polyphenols, accounting for about 8-12% of fresh leaf dry weight, and is the decisive component for forming green tea astringency taste, and also the main component of health effects of antioxidant, antibacterial, antiviral and anti-arteriosclerosis of tea beverage.
In this embodiment, the cutting branches of tea trees are separated from the parent of tea trees, the cutting is performed to cause a wound, the isolation between the polyphenols and the substrate PPO is broken, under appropriate conditions, oxidation occurs when oxygen is encountered to produce brown quinones, the quinones gradually diffuse into the whole nutrition chamber, and finally the branches are brown. Phenol oxidation reactions are often accompanied by strong free radical initiated side reactions, leading to uncertainty in the chemical reaction. In addition, the phenol oxidation reaction is difficult to control, the product selectivity is difficult to ensure, and the in-vitro preservation time of the tea tree cutting branches is not prolonged. In this embodiment, carbon dioxide is introduced from the bottom of the inner surface of the box body, the density of the carbon dioxide is higher than that of air, so that the nutrition chamber at the bottom of the inner surface of the box body is filled with enough carbon dioxide, oxygen is completely removed, contact between the cut of the tea tree cutting branch and the oxygen is avoided, and phenolic oxidation-reduction reaction occurs, so that the in-vitro preservation time of the tea tree branch is not facilitated to be prolonged.
In this embodiment, as shown in fig. 5, the planting plate is placed in the small round hole 701 on the planting plate 7, so that each plant of cutting branch is vertical, and each plant of cutting branch is guaranteed to absorb nutrient substances in the nutrition chamber.
In the embodiment, the water tank 11 is arranged in the center of the inside of the box body, liquid such as preservative is contained in the water tank, the water tank is arranged in the box body, the overall size of the device is reduced, the overall attractiveness of the device is guaranteed, the water suction pipe on the electric sprayer penetrates through the small round hole in the middle of the cover to enter the water tank in the box body, the power supply is connected, the motor drives the water pump to pump the liquid such as preservative in the water tank into the water pump of the electric sprayer, the liquid is sprayed into water mist with small particles through the nozzle 3, the tea tree cutting branches are preserved, meanwhile, the water mist gasifies and takes away heat, the indoor temperature is reduced, the activity of enzymes in the branches is inhibited, and therefore the respiration of the branches is inhibited.
In this embodiment, the internal surface design on box upper portion has a thermometer room 5, places the thermometer in thermometer room 5, monitors the internal temperature environment of box at any time, increases when the temperature is higher and sprays water smoke in to the box, reduces the internal temperature of box, suppresses the branch and carries out normal respiratory action, extension tea tree cuttage branch survival time in vitro.
Example 3
According to the size and function requirements of the present invention, a carbon dioxide sensor with a model SPG30 is selected, and fig. 6 shows a carbon dioxide module 14 according to an embodiment of the present invention, including a carbon dioxide storage chamber 1401, a control system box 1402, a carbon dioxide air inlet pipe 1403, and a carbon dioxide sensor 13.
In this embodiment, the carbon dioxide module is installed in a carbon dioxide chamber provided at the bottom of the device of the present invention, the carbon dioxide sensor is installed in a groove at the middle position of the water tank 11, the gas environment in the tank is detected, the detected carbon dioxide concentration signal is transmitted back to the system control box along the line of the water tank, and after the system control box receives the signal fed back by the sensor, the system control box controls the start and stop of the carbon dioxide storage chamber, and controls the carbon dioxide content discharged into the tank.
In this embodiment, through discharging into the air environment in the carbon dioxide change box in the box, with the majority air in the box discharge box, reduce the oxygen content in the box, oxygen content reduces, suppresses tea tree cuttage branch self respiration, reduces the nutrient substance consumption of branch itself.
In this example, carbon dioxide is introduced into the tank, and the density of the gaseous carbon dioxide is 1.977kg/m 3 Air density of 1.29kg/m 3 The density of the carbon dioxide is higher, the carbon dioxide is introduced through the bottom of the box body, the air at the bottom of the box body can be completely discharged, at the moment, the nutrition chamber at the bottom of the box body basically does not contain oxygen, and the phenolic substances at the notch of the branch are prevented from being combined with the oxygen to oxidizeAnd (3) reduction reaction.
Example 4
The invention further aims to provide a use method of the device for prolonging the in-vitro survival time of the tea tree germplasm resource cuttage branches.
In this embodiment, the upper cover of the device for prolonging the in-vitro survival time of the cuttage branches of the tea tree germplasm resources is opened, the planting plate is taken out, nutrient substances required by in-vitro culture of the cuttage branches are added into the nutrition chamber, and then the planting plate is installed at the upper end of the nutrition chamber.
In this embodiment, after the cutting branches of the tea tree are separated from the parent of the tea tree, the fresh-keeping treatment is immediately performed on the branches, and the branches are immediately placed in the small round holes on the planting plate after the fresh-keeping treatment, and then the cover is covered.
In this embodiment, start carbon dioxide control system case after covering the lid, let in carbon dioxide to the box in, discharge box internal portion air, reduce the oxygen content in the box, restrain branch respiratory function.
In this embodiment, touching the electric atomizer switch starts electric atomizer, draws liquid from the water tank, erupts liquid into granule water smoke entering box through the nozzle, and the heat in the box is taken away in the gasification of liquid granule.
In summary, after the tea tree cutting branches separated from the tea tree parent are subjected to fresh-keeping treatment, the tea tree cutting branches are placed on the fixed planting plate in the device, and the fixed planting plate is fully distributed with small holes, so that the cutting branches are vertically placed in the small holes, the mutual accumulation and extrusion of the branches are avoided, each plant branch is ensured to absorb nutrient substances in a nutrition room, and each plant branch is ensured to be kept fresh by water mist sprayed by an electric sprayer in the transportation process; the bottom in the device is provided with a nutrition chamber for containing nutrition liquid required by the branches, so that the nutrition substances required by the branches are maintained after the parent substances are separated; the bottom of the device is provided with the carbon dioxide storage chamber which is communicated with the inside of the device, and carbon dioxide is discharged from the bottom of the device to the inside of the device, so that the oxygen content in the device is reduced, the respiration of branches is inhibited, and the consumption of organic matters stored by the branches is reduced; the electric spraying device is designed on the top cover, the water tank is arranged in the center of the inside of the device, the electric sprayer sucks liquid in the water tank through the water pipe, and then the liquid is sprayed into small-particle water mist through the nozzle, so that the water mist not only can keep fresh cutting branches, but also takes away heat in the device when the water mist is gasified, the temperature in the box is reduced, the activity of respiratory enzymes in the branches is affected when the temperature is reduced, the self respiration of the tea tree cutting branches is inhibited, the consumption of organic matters is reduced, and the survival rate of the cutting branches is increased.
Claims (5)
1. The utility model provides a device of extension tea tree germplasm resources cuttage branch survival time in vitro, includes electric nebulizer (2), lid (4), box (6), carbon dioxide module (14), its characterized in that: the electric sprayer (2) is arranged above the cover (4), the cover (4) covers the upper end of the box body (6), and the carbon dioxide module (14) is arranged at the bottom of the box body (6); the miniature suction pump of electric nebulizer (2) model for USB, five little round holes have been seted up on lid (4), box (6) internal design has water tank (11), and the water pipe of electric nebulizer (2) passes little round hole at the very center of lid (2) stretches into box (6) internal water tank (11), and four nozzles of electric nebulizer (2) pass in remaining four little round holes on lid (4) stretch into box (6), start electric nebulizer (2), the water that holds in water tank (11) is drawn to the water pipe, utilizes four nozzles to spray water into small granule water smoke, and box (6) internal heat is taken away in the water smoke gasification.
2. The device for prolonging the in-vitro survival time of tea tree germplasm resource cutting branches according to claim 1, wherein the device comprises: nutrient substances required by the growth of branches in the nutrition room (8) are designed in the inner bottom of the box body (6).
3. The device for prolonging the in-vitro survival time of tea tree germplasm resource cutting branches according to claim 2, which is characterized in that: the planting plate (7) is arranged on the nutrition room (8), a plurality of small holes (701) and a central hole (702) are formed in the planting plate (7), branches are separated from tea tree precursors, and the branches are vertically placed in the small holes (701) after fresh-keeping treatment.
4. The device for prolonging the in-vitro survival time of tea tree germplasm resource cutting branches according to claim 1, wherein the device comprises: the inner surface of the box body (6) is provided with a thermometer chamber (5), and a thermometer is placed in the thermometer chamber (5) to detect the temperature in the box body in real time.
5. The device for prolonging the in-vitro survival time of tea tree germplasm resource cutting branches according to claim 1, wherein the device comprises: carbon dioxide module (14) is by carbon dioxide apotheca (1401), control system case (1402), carbon dioxide intake pipe (1403), carbon dioxide sensor (13), the carbon dioxide room has been designed to box (6) bottom, carbon dioxide module (14) are installed in the carbon dioxide room, carbon dioxide sensor (13) are installed in water tank (11) intermediate position detection box body's carbon dioxide content, the carbon dioxide concentration signal that detects is along with the circuit transmission of water tank cloth accuse back to system control case (1402), system control case (1402) control carbon dioxide apotheca (1401) start and stop, the carbon dioxide of control row income box (6), with the partial air discharge in box (6), reduce the oxygen content in box (6).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310723659.9A CN116918583B (en) | 2023-06-19 | 2023-06-19 | Device and method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches |
| LU505683A LU505683B1 (en) | 2023-06-19 | 2023-12-01 | A DEVICE AND METHOD FOR EXTENDING THE IN VITRO SURVIVAL TIME OF CUTTINGS OF TEA TREE GERMPLASM RESOURCES |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310723659.9A CN116918583B (en) | 2023-06-19 | 2023-06-19 | Device and method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116918583A true CN116918583A (en) | 2023-10-24 |
| CN116918583B CN116918583B (en) | 2024-04-09 |
Family
ID=88393298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310723659.9A Active CN116918583B (en) | 2023-06-19 | 2023-06-19 | Device and method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN116918583B (en) |
| LU (1) | LU505683B1 (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030011605A (en) * | 2001-07-26 | 2003-02-11 | 베르그 아스 가부시키가이샤 | Storage method and pre-acclimation method for grafted nursery plant with no root, grafted nursery plant obtained with the method above, and device used for the method above |
| CN104542924A (en) * | 2015-01-04 | 2015-04-29 | 华南农业大学 | Fruit and vegetable fresh-keeping method |
| CN205682112U (en) * | 2016-06-20 | 2016-11-16 | 李红光 | A kind of portable cuttage branch case |
| CN206118493U (en) * | 2016-09-29 | 2017-04-26 | 伊犁哈萨克自治州农业科学研究所 | Portable scion bin that keeps fresh |
| CN206949400U (en) * | 2017-04-27 | 2018-02-02 | 广州聚联物流有限公司 | A kind of air-conditioning fresh-keeping device |
| CN207948247U (en) * | 2018-01-26 | 2018-10-12 | 庆元县实验林场 | A kind of Phoebe bournei branch save set |
| CN109673292A (en) * | 2019-01-31 | 2019-04-26 | 中国农业科学院蔬菜花卉研究所 | A kind of vegetables scion crisper |
| CN213785052U (en) * | 2020-12-11 | 2021-07-27 | 南京林业大学 | Wild germplasm resources's of matrimony vine save set |
| CN114568310A (en) * | 2022-04-12 | 2022-06-03 | 云南省农业科学院茶叶研究所 | Method for prolonging survival time of cuttage branches of tea plant germplasm resources in vitro |
| CN216762925U (en) * | 2021-11-10 | 2022-06-17 | 广西壮族自治区中国科学院广西植物研究所 | Precious plant cuttage branch save set |
| CN217089304U (en) * | 2022-04-22 | 2022-08-02 | 宣恩县孔氏茶业有限公司 | A female branch storage container for tea tree cuttage |
| CN114932934A (en) * | 2022-04-28 | 2022-08-23 | 南昌工程学院 | Camphor tree cutting field preservation and transportation device |
| CN217806433U (en) * | 2022-06-15 | 2022-11-15 | 云南农业大学 | A fresh-keeping device for tea tree cuttage branch |
-
2023
- 2023-06-19 CN CN202310723659.9A patent/CN116918583B/en active Active
- 2023-12-01 LU LU505683A patent/LU505683B1/en active IP Right Grant
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030011605A (en) * | 2001-07-26 | 2003-02-11 | 베르그 아스 가부시키가이샤 | Storage method and pre-acclimation method for grafted nursery plant with no root, grafted nursery plant obtained with the method above, and device used for the method above |
| CN104542924A (en) * | 2015-01-04 | 2015-04-29 | 华南农业大学 | Fruit and vegetable fresh-keeping method |
| CN205682112U (en) * | 2016-06-20 | 2016-11-16 | 李红光 | A kind of portable cuttage branch case |
| CN206118493U (en) * | 2016-09-29 | 2017-04-26 | 伊犁哈萨克自治州农业科学研究所 | Portable scion bin that keeps fresh |
| CN206949400U (en) * | 2017-04-27 | 2018-02-02 | 广州聚联物流有限公司 | A kind of air-conditioning fresh-keeping device |
| CN207948247U (en) * | 2018-01-26 | 2018-10-12 | 庆元县实验林场 | A kind of Phoebe bournei branch save set |
| CN109673292A (en) * | 2019-01-31 | 2019-04-26 | 中国农业科学院蔬菜花卉研究所 | A kind of vegetables scion crisper |
| CN213785052U (en) * | 2020-12-11 | 2021-07-27 | 南京林业大学 | Wild germplasm resources's of matrimony vine save set |
| CN216762925U (en) * | 2021-11-10 | 2022-06-17 | 广西壮族自治区中国科学院广西植物研究所 | Precious plant cuttage branch save set |
| CN114568310A (en) * | 2022-04-12 | 2022-06-03 | 云南省农业科学院茶叶研究所 | Method for prolonging survival time of cuttage branches of tea plant germplasm resources in vitro |
| CN217089304U (en) * | 2022-04-22 | 2022-08-02 | 宣恩县孔氏茶业有限公司 | A female branch storage container for tea tree cuttage |
| CN114932934A (en) * | 2022-04-28 | 2022-08-23 | 南昌工程学院 | Camphor tree cutting field preservation and transportation device |
| CN217806433U (en) * | 2022-06-15 | 2022-11-15 | 云南农业大学 | A fresh-keeping device for tea tree cuttage branch |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116918583B (en) | 2024-04-09 |
| LU505683B1 (en) | 2024-06-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11382345B2 (en) | Selenium-rich wheat whole-sprout juice beverage and method for preparation thereof | |
| Li et al. | Sea buckthorn (Hippophae rhamnoides L.): production and utilization | |
| CN108094175A (en) | A kind of fibert epicormic branch cutting method | |
| CN108668658A (en) | A kind of Damask Rose cuttage and seedling culture method | |
| CN104012310A (en) | Method for grafting and raising a seedling of melon | |
| CN106718735A (en) | A kind of method for planting dendrobium candidum in the tree | |
| CN109315356A (en) | One kind being suitable for aleyrodid class or parasitic wasp class insects hatch, feeding device and its application method | |
| CN106034721A (en) | Planting method of brassica oleracea sprouting vegetables | |
| CN108834799A (en) | A kind of implantation methods of nature selenium-rich pueraria lobata | |
| CN107836157B (en) | Peanut sprout and production method thereof | |
| CN105359776A (en) | Method for mulching film seedling culture and planting of purple potatoes | |
| CN109329019B (en) | Method for water culture of moringa oleifera vegetables | |
| CN116918583B (en) | Device and method for prolonging in-vitro survival time of tea tree germplasm resource cuttage branches | |
| CN106748362A (en) | A kind of method for planting dendrobium candidum in the tree | |
| KR20210041803A (en) | The Production Process for Codonopsis lanceolata | |
| CN107306782B (en) | Water planting method of water spinach rich in trace elements | |
| CN106069159B (en) | The inoculation method of blood aspidistra | |
| CN107736203A (en) | A kind of as if field winter bamboo shoot implantation methods | |
| CN108012923A (en) | A kind of cultural method of selenium-rich roxburgh anoectochilus terminal bud | |
| CN110583463A (en) | Cloud and mist cultivation method for peppers | |
| CN110627573A (en) | Method for preparing special fertilizer for improving soil hardening by using tea milli as raw material | |
| CN111771610A (en) | Box type Dictyophora rubrovalvata cultivation device and method | |
| CN110452800A (en) | A kind of passion fruit starfruit wine and preparation method thereof | |
| CN109906930A (en) | A kind of planting unit and manufacture craft and application method based on bamboo living | |
| CN108634068A (en) | Tea root tea and its processing technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |