CN116769605A - Solid propagation inoculation method for mushrooms - Google Patents
Solid propagation inoculation method for mushrooms Download PDFInfo
- Publication number
- CN116769605A CN116769605A CN202210221218.4A CN202210221218A CN116769605A CN 116769605 A CN116769605 A CN 116769605A CN 202210221218 A CN202210221218 A CN 202210221218A CN 116769605 A CN116769605 A CN 116769605A
- Authority
- CN
- China
- Prior art keywords
- fungus
- strain
- basal
- bag
- inoculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000007787 solid Substances 0.000 title claims abstract description 18
- 238000011081 inoculation Methods 0.000 title claims abstract description 17
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 16
- 241000233866 Fungi Species 0.000 claims abstract description 35
- 239000002245 particle Substances 0.000 claims abstract description 17
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 238000009423 ventilation Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 241001391944 Commicarpus scandens Species 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mushroom Cultivation (AREA)
Abstract
A mushroom solid propagation inoculation method, which comprises the following steps: taking out the basal column strain cultured by the solid particle culture medium through the honeycomb culture device, putting the basal column strain into a fungus hole corresponding to the fungus bag, and inoculating the aseptic material bag in a conventional aseptic environment; and step two, sealing and culturing in a conventional mode. The fungus solid propagation inoculation method of the application forms a basal column-shaped fungus composed of particles in a honeycomb body, the whole or a section is inserted into a fungus bag, the volume is larger than that of the particle fungus, the fungus is not easy to break in use, the fungus has large quantity of fungus, the manufacture is convenient, the fungus is not easy to pollute, the quality is high, the fungus is easy to germinate, the mechanized operation is convenient, and the fungus quality can be improved.
Description
Technical Field
The application relates to the field of microbial strain propagation.
Background
Microorganisms including bacteria, viruses, fungi, mushrooms, small protozoa, microalgae and the like are a large group of organisms, and the microorganisms are widely applied to various fields of foods, medicines, industry and agriculture, environmental protection and the like, so that many biologists in society are constantly devoted to culture, propagation and research of microorganisms.
The microbial strain propagation is a basic technology widely used in the fields of microbiology, biomedicine and microbiology, when the microbial strain is subjected to expansion culture, namely propagation, a culture medium for microbial culture is firstly selected, then the culture medium is inoculated and cultured, and finally the cultured strain is used for the expansion culture and propagation in quantity, for example, the mushroom propagation process is mother strain-stock strain-cultivated strain, the strain is generally divided into three stages, namely, the first stage is mother strain, the second stage is stock strain, the third stage is cultivated strain, the first stage to the third stage is for propagation of the strain, and the conventional mushroom propagation technology comprises the following steps: (1) In the whole propagation process, the strain is crushed and fine through multiple particle separation, so that hypha strain is caused, as shown in fig. 1 and 2, grain particles are inoculated and propagated into grain particle strain 1, a plurality of particle culture mediums are filled in a large container for culturing and seed production, hypha particles are taken out, and hypha strain is caused in the process of separating hypha particles, and the hypha strain is easy to pollute in multiple operations; (2) The mycelium growth culture medium is solid whole, such as ice cream stick and branch strain, which is not aerated, has few matrix mycelium and more aerial mycelium, resulting in poor mycelium quality, as shown in fig. 3 and 4, the amount of mycelium in solid wood is small, and mycelium grows on the surface and the germination is weak; (3) The method has high requirements and poor adaptability by adopting liquid strain propagation. In conclusion, the prior art causes time and labor consumption in the whole strain manufacturing and inoculating process, and the strain quality is poor.
Disclosure of Invention
In order to solve the problems of the traditional mushroom propagation inoculation method, the application provides a mushroom solid propagation inoculation method.
The technical scheme adopted by the application for achieving the purpose is as follows: a mushroom solid propagation inoculation method, which comprises the following steps:
taking out the solid particle culture medium through the basal column strain 26 cultured by the honeycomb culture device, putting the basal column strain into a fungus hole corresponding to the fungus bag 2, and inoculating the aseptic material bag in a conventional aseptic environment;
and step two, sealing and culturing in a conventional mode.
The whole or a segment of the basal column strain 26 is put into the corresponding fungus hole of the fungus bag 2.
In the first step, the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%.
The culture device consists of a honeycomb body 5, an upper ventilation cover 4 and a lower ventilation cover 6, wherein a strain hole 8 which is vertically communicated is arranged in the honeycomb body 5.
The specification of the basal columnar strain 26 is 3 x 18cm.
The fungus solid propagation inoculation method of the application forms a basal column-shaped fungus composed of particles in a honeycomb body, the whole or a section is inserted into a fungus bag, the volume is larger than that of the particle fungus, the fungus is not easy to break in use, the fungus has large quantity of fungus, the manufacture is convenient, the fungus is not easy to pollute, the quality is high, the fungus is easy to germinate, the mechanized operation is convenient, and the fungus quality can be improved.
Drawings
FIG. 1 is a schematic diagram of conventional grain particle breeding.
FIG. 2 is a schematic diagram of a conventional grain seed strain planting.
Figure 3 is a schematic diagram of a prior art shoot breeding.
FIG. 4 is a schematic representation of a prior art shoot strain planting.
FIG. 5 is a front view showing an exploded view of the culture apparatus for propagation of mushroom according to the present application.
FIG. 6 is a schematic sectional exploded view of a culture apparatus for propagation of fungus according to the present application.
FIG. 7 is a schematic cross-sectional view showing the propagation state of a culture apparatus for propagation of fungus according to the present application.
FIG. 8 is a front view showing the structure of the culture apparatus according to the present application in a strain-removed state.
FIG. 9 is a schematic diagram showing the state of the strain inserted into the fungus bag according to the first embodiment.
FIG. 10 is a schematic diagram showing the state of the second strain inserted into the bag.
In the figure: 1. grain particle strain 2, fungus bag 3, branch strain 4, upper ventilation cover 5, honeycomb body 6, lower ventilation cover 7, clamping groove 8, strain hole 26, basal column strain.
Detailed Description
As shown in fig. 5 and 6, the culture device for mushroom propagation of the application comprises an upper ventilation cover 4, a honeycomb body 5 and a lower ventilation cover 6, wherein a strain hole 8 is vertically and vertically penetrated in the cylindrical honeycomb body 5, the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged at the upper part and the lower part of the honeycomb body 5, and the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged on the honeycomb body 5 through convex clamps and clamping grooves 7 arranged on the upper ventilation cover 4 and the lower ventilation cover 6.
The honeycomb body 5 is provided with a plurality of strain holes 8, and is cultivated by adopting an integrated chamber to form a basal column strain composed of particles, so that the strain is not easy to break hypha in use, and the strain has large hypha quantity, convenient manufacture, difficult pollution, high quality and easy germination.
The using method is as follows:
step one, conventionally preparing a culture medium, wherein the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%;
filling culture mediums into the strain holes 8 respectively and compacting;
step three, covering the upper ventilation cover 4 and the lower ventilation cover 6;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the upper ventilation cover 4 or/and the lower ventilation cover 6 under the aseptic condition, and inoculating strains in the strain holes 8 respectively;
step six, covering an upper ventilation cover 4 and a lower ventilation cover 6 after inoculation is finished, and culturing in a conventional mode;
step seven, culturing the strain, wherein the strain is in a basal column shape and has the specification of 3-18 cm; as shown in fig. 7, the upper ventilation cap 4 or the lower ventilation cap 6 is opened in a sterile environment, and the basal column strain 26 is taken out and placed in the fungus bag 2 for inoculation.
After culturing the strain, the lid is opened in a sterile environment, and the whole of the basal pillar-like strain 26 is taken out at either end of the honeycomb body 5, as shown in FIG. 8. The aseptic package is inoculated in a conventional aseptic environment, and the basal column strains 26 consisting of the granular culture medium are directly inserted into corresponding bacterial holes of the package 1, as shown in fig. 9. Or the basal column strains 26 consisting of the granular culture medium are segmented and connected into corresponding strain holes of the material packet, as shown in figure 10. Sealing and culturing conventionally.
The strain propagated by the application is obviously superior to the liquid strain, the branch solid wood strain and the grain strain in the prior art in the eating time, the germination time and the full package time after inoculation, and the pollution rate is obviously lower than that of the prior art. At the same time, the disease resistance, stress resistance and adaptability are all improved.
Comparison table of germination time and pollution rate of fungus bags after inoculation
As shown in the table, the germination time and the bag filling time of the basal column particle strain are less than those of other comparative strain propagation modes, and the pollution rate is obviously lower than that of other propagation modes.
The present application has been described in terms of embodiments, and it will be appreciated by those of skill in the art that various changes can be made to the features and embodiments, or equivalents can be substituted, without departing from the spirit and scope of the application. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the application without departing from the essential scope thereof. Therefore, it is intended that the application not be limited to the particular embodiment disclosed, but that the application will include all embodiments falling within the scope of the appended claims.
Claims (5)
1. A mushroom solid propagation inoculation method is characterized in that: the method comprises the following steps:
taking out the solid particle culture medium through a basal column strain (26) cultured by a honeycomb culture device, putting the basal column strain into a fungus hole corresponding to a fungus bag (2), and inoculating the aseptic material bag in a conventional aseptic environment;
and step two, sealing and culturing in a conventional mode.
2. The method for solid propagation and inoculation of mushroom according to claim 1, wherein the method comprises the following steps: the whole or the segments of the basal column strain (26) are put into the corresponding strain holes of the strain bag (2).
3. The method for solid propagation and inoculation of mushroom according to claim 1, wherein the method comprises the following steps: in the first step, the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%.
4. The method for solid propagation and inoculation of mushroom according to claim 1, wherein the method comprises the following steps: the culture device consists of a honeycomb body (5), an upper ventilation cover (4) and a lower ventilation cover (6), wherein a strain hole (8) which is vertically communicated is arranged in the honeycomb body (5).
5. The method for solid propagation and inoculation of mushroom according to claim 1, wherein the method comprises the following steps: the specification of the basal columnar strain (26) is 3 x 18cm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210221218.4A CN116769605A (en) | 2022-03-09 | 2022-03-09 | Solid propagation inoculation method for mushrooms |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210221218.4A CN116769605A (en) | 2022-03-09 | 2022-03-09 | Solid propagation inoculation method for mushrooms |
Publications (1)
Publication Number | Publication Date |
---|---|
CN116769605A true CN116769605A (en) | 2023-09-19 |
Family
ID=87994975
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210221218.4A Pending CN116769605A (en) | 2022-03-09 | 2022-03-09 | Solid propagation inoculation method for mushrooms |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN116769605A (en) |
-
2022
- 2022-03-09 CN CN202210221218.4A patent/CN116769605A/en active Pending
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109234176A (en) | Cordyceps sinensis mother culture media and preparation method thereof | |
CN116769605A (en) | Solid propagation inoculation method for mushrooms | |
CN111088169B (en) | Trichoderma, microbial agent and application thereof | |
CN209931098U (en) | Edible mushroom culture apparatus | |
KR101239641B1 (en) | Strain of Novel White Flammulina velutipes | |
CN116769604A (en) | Method for propagating and storing mushroom | |
CN116769606A (en) | Method for breeding and producing seeds of mushroom | |
CN219834975U (en) | Honeycomb culture body for mushroom propagation | |
CN1944625B (en) | Coterpillar fungus hypha cultivating method of rice solid fermenting for caterpillar fungus drink | |
CN217709437U (en) | A culture apparatus for mushroom expands numerous | |
CN116769569A (en) | Culture device and method for mushroom propagation | |
CN103202174A (en) | Method for ecologically cultivating edible fungi | |
CN112143656B (en) | Preparation method of yellow tumor spore powder | |
KR102183105B1 (en) | Production method of mushroom using mushroom cultivation bag with micropore | |
CN1265697C (en) | Edible mushroom cultivating and producing basket and basket type cultivating method | |
CN1025790C (en) | New Mushroom-seed culturing process | |
CN112806215A (en) | Method for preparing mushroom liquid strain and method for producing mushroom stick | |
CN105393793A (en) | Bag cultivation Pleurotus ostreatus simple and fast hypha running method | |
CN1543766A (en) | Edible mushroom capsule and capsule mushroom and production method thereof | |
CN116491366B (en) | Ganoderma lucidum cultivation method capable of reducing heavy metal ion enrichment | |
CN112457997B (en) | Solid fermentation method for trichoderma | |
CN103340090A (en) | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer | |
CN1148112C (en) | Equipment and technology for producing edible fungus spawn | |
CN1952109A (en) | Short term storage method for large-size fungus species | |
JPS61249322A (en) | Artificial culture medium of mushroom |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |