CN103202174A - Method for ecologically cultivating edible fungi - Google Patents
Method for ecologically cultivating edible fungi Download PDFInfo
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- CN103202174A CN103202174A CN 201210009192 CN201210009192A CN103202174A CN 103202174 A CN103202174 A CN 103202174A CN 201210009192 CN201210009192 CN 201210009192 CN 201210009192 A CN201210009192 A CN 201210009192A CN 103202174 A CN103202174 A CN 103202174A
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- blake bottle
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- 238000000034 method Methods 0.000 title claims abstract description 38
- 241000233866 Fungi Species 0.000 title claims abstract description 11
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 29
- 239000002023 wood Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241000209094 Oryza Species 0.000 claims description 11
- 235000007164 Oryza sativa Nutrition 0.000 claims description 11
- 235000009566 rice Nutrition 0.000 claims description 11
- 244000168667 Pholiota nameko Species 0.000 claims description 8
- 235000014528 Pholiota nameko Nutrition 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 235000005340 Asparagus officinalis Nutrition 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 244000003416 Asparagus officinalis Species 0.000 claims 1
- 238000009423 ventilation Methods 0.000 abstract description 8
- 239000002245 particle Substances 0.000 abstract description 6
- 239000001963 growth medium Substances 0.000 abstract description 4
- 239000002609 medium Substances 0.000 description 32
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000012364 cultivation method Methods 0.000 description 4
- 239000010410 layer Substances 0.000 description 3
- 241000234427 Asparagus Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a method for ecologically cultivating edible fungus mycelia. The method comprises the step of sequentially filling four layers of culture media with different particle sizes from bottom to top in a cylindrical culture bottle. The high-yield edible fungi can be cultivated without ventilation operation. The invention also relates to a method for ecologically cultivating edible fungi, and comprises the step of the method for ecologically cultivating edible fungus mycelia.
Description
Technical field
The invention belongs to the cultivation field of edible mushroom (macro fungi), particularly, the present invention relates to be used in the method that the layering medium is cultivated edible mushroom in the cylindrical blake bottle, wherein need not to be taken a breath in medium inside.
Technical background
Edible mushroom generally all is to plant by artificial cultivation now.At present general in culture vessel the solid culture medium with homogeneous add water, to wherein evenly inoculating mycelium at interval, make the mycelium diffusion grow.Wherein in the less cylindrical blake bottle of volume, cultivate, because the cross growth diffusion suffers restraints, make the edible mushroom of strip, for example Asparagus, dried mushroom, pholiota nameko etc., than in more open culture tank, cultivating, to grow more straight and upright attractive in appearancely, and straight and upright thalline is easier to the later stage cleaning.
Yet, the inventor finds, because the relative more open container volume of blake bottle is much smaller, wherein medium evenly distributes, the consistent in density of each several part, thereby the elongation of mycelia is also even, the carbon dioxide that causes metabolism to produce runs into the moisture of medium and produces carbonic acid, the lower floor that can be diffused in medium rapidly spreads all over the mycelial direction of growth in the upper strata, thereby influences mycelial growth.Therefore, during mycelial growth, must often take a breath, with lax medium, otherwise incur loss through delay ventilation as vibrations trainings bottle, gently then influence mycelial growth, influence output, heavy then can cause mycelia to die off.And the blake bottle that quantity is many for volume is little ventilation, suitable labor intensive, this has restricted on the contrary with the popularization of cultivating the edible mushroom technology in the blake bottle, be unfavorable for improving farming the added value of edible mushroom carrier.
The inventor is through long-term practice and arduous research, take a hint by the natural environment research to edible fungi growth, discovery is filled medium by initial layering and is gone into blake bottle, can in the edible mushroom mycelium process of growth, need not the ventilation operation, also can cultivate the edible mushroom of high yield, such padding has significantly reduced the workload of later stage ventilation operation, makes with easier popularization of technology of cultivating edible mushroom in the blake bottle.Certainly, the complete natural imitation ecotope of present layering packing ratio will be operated manyly easily.
Summary of the invention
The technical problem to be solved in the present invention is, the method for new artificial cultivation edible mushroom mycelium and the method for artificial cultivation edible mushroom are provided, and wherein need not the ventilation operation in the edible mushroom mycelium process of growth.
Particularly, the invention provides the mycelial method of culturing edible fungus, it comprises,
(1) in cylindrical blake bottle, filling granularity from bottom to up successively is the medium 1 of 5～10 orders (being preferably 6～8 orders), the medium 2 of 18～33 orders (being preferably 20～30 orders), the medium 3 of 38～53 orders (being preferably 40～50 orders) and the medium 4 of 38～53 orders (being preferably 40～50 orders), and with bacterial classification inoculation between medium 3 and medium 4; With
(2) cultivate in 15～28 ℃ (being preferably 16～22 ℃).
The inventor discovers that by varigrained culture medium layer, lower floor's particle is bigger, mutually between bigger hole be of value to the growth at mycelium initial stage; Varigrained culture medium layer like this can reduce the convection current of internal environment, thereby makes carbonic acid gas effectively be accumulated in the lower floor of medium inside, and the level height when less rising to mycelium and upwards extending, the elongation that helps mycelium to make progress.
Preferably in the method for artificial cultivation edible mushroom mycelium, medium 1 and/or medium 2 comprise wood chip, rice bran and water, and wherein the weight ratio of wood chip, rice bran and water is 1～3: 0.5～1.5: 4～8, be preferably 2: 1: 6.More preferably wherein, the volume that medium 1 and/or medium 2 are filled accounts for 15%～25% of blake bottle volume, is preferably 20%.
Preferably in the method for artificial cultivation edible mushroom mycelium, medium 3 comprises wood chip, rice bran and water, and wherein the weight ratio of wood chip, rice bran and water is 2～4: 0.5～1.5: 6～10, be preferably 3: 1: 8.More preferably wherein the volume of medium 3 fillings accounts for 15%～25% of blake bottle volume, is preferably 20%.
Preferably in the method for artificial cultivation edible mushroom mycelium, medium 4 comprises wood chip.More preferably wherein the volume of medium 4 fillings accounts for 30%～50% of blake bottle volume, is preferably 40%.
Preferably in the method for artificial cultivation edible mushroom mycelium, edible mushroom is Asparagus, dried mushroom or pholiota nameko, preferably pholiota nameko.
Preferably in the method for artificial cultivation edible mushroom mycelium, the volume of blake bottle is 0.8～1.2L, is preferably 1～1.1L.
In addition, the present invention also provides the method for culturing edible fungus, and it comprises,
(a) method of enforcement artificial cultivation edible mushroom mycelium of the present invention; With
(b) mycelium of incubation step (a) acquisition obtains edible mushroom.
The beneficial effect that the present invention obtains is: in the cultivation of the cylindrical blake bottle of using smaller size smaller, need not the ventilation operation in the edible mushroom mycelium process of growth, just can cultivate the edible mushroom of high yield, reduce the workload of later stage ventilation operation, attractive in appearance, the homogeneous of the edible mushroom of turning out.
Embodiment
Get wood chip and the rice bran of sterilization, wood chip and the rice bran weight ratio according to 2: 1 is mixed, be ground into different grain size then, collect the medium of following particle size range: (a) 6～8 orders (can sieve by 6 mesh sieves but held back by 8 mesh sieves); (b) 20～30 orders (can sieve by 20 mesh sieves but held back by 30 mesh sieves); Wood chip and the rice bran weight ratio according to 3: 1 is mixed, pulverize and collect the medium of following particle size range then: (c) 40～50 orders (can sieve by 40 mesh sieves but held back by 50 mesh sieves); Wood chip is pulverized and is collected the medium of following particle size range: (d) 40～50 orders (can sieve by 40 mesh sieves but held back by 50 mesh sieves).
Fill medium as follows to the blake bottle (interior diameter is 3cm, is 150cm highly, and volume is about 1L) of cylinder open then: (1) is packed into undermost 1/5 height of blake bottle after medium (a) and water are mixed with 1: 2 weight ratio; (2) medium (b) and water are mixed with 1: 2 weight ratio after, then be packed into 1/5 height of blake bottle; Then, after medium (c) and water mixed with 1: 1 weight ratio, then be packed into 1/5 height of blake bottle; At last, broadcast the pholiota nameko bacterial classification at this laminar surface layer earlier, then medium (d) is packed into 2/5 height of the blake bottle the superiors, to cover bacterial classification.
Blake bottle is moved into culturing room cultivates with 20 ℃, 50% relative moisture, during the operation of not taking a breath, be that visible mycelia extends the blake bottle open surfaces to 23～25 days, again through cutting mycoderm in 50～60 days, humidity is increased to 85%, wait for the growth fruiting, results.
(method 1: existing culture tank is cultivated, and wherein uses the 1L medium for cultivation method of the present invention and additive method; Method 2: substantially according to the method described above, difference is that the particle size range of each layer is 20～30 orders) compare, the result is as shown in table 1 below, show that cultivation method of the present invention is suitable with prior art on output, but quality is much better; And if with the granularity of homogeneous and do not take a breath, will have a strong impact on output.
The effect comparison sheet of table 1 cultivation method of the present invention and additive method
| Cultivation method of the present invention | Method 1 | Method 2 | |
| The fate that mycelial growth goes out | 23～25 days | 28～33 days | 30～35 days |
| Pholiota nameko harvest yield (weight in wet base, | 186～208 grams | 179～210 grams | 84～135 grams |
| In the 1L medium) | |||
| The pholiota nameko quality | Straight and upright, good uniformity | Morphological differences is big | Straight and upright, good uniformity |
The present invention is described by embodiment.Those skilled in the art can use for reference content appropriate change technical characterictic of the present invention and realize corresponding purpose, relevant change does not all break away from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and all be regarded as comprising within the scope of the present invention.
Claims (10)
1. the mycelial method of culturing edible fungus, it comprises,
(1) in cylindrical blake bottle, filling granularity from bottom to up successively is the medium 1 of 5～10 orders (being preferably 6～8 orders), the medium 2 of 18～33 orders (being preferably 20～30 orders), the medium 3 of 38～53 orders (being preferably 40～50 orders) and the medium 4 of 38～53 orders (being preferably 40～50 orders), and with bacterial classification inoculation between medium 3 and medium 4; With
(2) cultivate in 15～28 ℃ (being preferably 16～22 ℃).
2. the described method of claim 1, wherein medium 1 and/or medium 2 comprise wood chip, rice bran and water, wherein the weight ratio of wood chip, rice bran and water is 1～3: 0.5～1.5: 4～8, be preferably 2: 1: 6.
3. the described method of claim 2, wherein medium 1 and/or medium 2 volume of filling accounts for 15%～25% of blake bottle volume, is preferably 20%.
4. the described method of claim 1, wherein medium 3 comprises wood chip, rice bran and water, wherein the weight ratio of wood chip, rice bran and water is 2～4: 0.5～1.5: 6～10, be preferably 3: 1: 8.
5. the described method of claim 4, wherein medium 3 volume of filling accounts for 15%～25% of blake bottle volume, is preferably 20%.
6. the described method of claim 1, wherein medium 4 comprises wood chip.
7. the described method of claim 6, wherein medium 4 volume of filling accounts for 30%～50% of blake bottle volume, is preferably 40%.
8. the described method of claim 1, wherein edible mushroom is Asparagus, dried mushroom or pholiota nameko, preferably pholiota nameko.
9. the described method of claim 1, wherein the volume of blake bottle is 0.8～1.2L, is preferably 1～1.1L.
10. the method for culturing edible fungus, it comprises,
(a) implement the described method of claim 1; With
(b) mycelium of incubation step (a) acquisition obtains edible mushroom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210009192 CN103202174A (en) | 2012-01-13 | 2012-01-13 | Method for ecologically cultivating edible fungi |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210009192 CN103202174A (en) | 2012-01-13 | 2012-01-13 | Method for ecologically cultivating edible fungi |
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| Publication Number | Publication Date |
|---|---|
| CN103202174A true CN103202174A (en) | 2013-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210009192 Pending CN103202174A (en) | 2012-01-13 | 2012-01-13 | Method for ecologically cultivating edible fungi |
Country Status (1)
| Country | Link |
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| CN (1) | CN103202174A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104756751A (en) * | 2014-01-07 | 2015-07-08 | 陕西省微生物研究所 | Edible fungi bag cultivation technology based on natural rain simulation |
| CN105900806A (en) * | 2016-04-22 | 2016-08-31 | 固镇县绿禾家庭农场 | Planting method for high-yield pollution-free cucumbers |
| CN106688603A (en) * | 2015-08-11 | 2017-05-24 | 天津市庆祥农业科技有限公司 | Preparation method of edible mushrooms and process thereof |
| CN107637382A (en) * | 2017-09-29 | 2018-01-30 | 永州市祥瑞生物科技有限公司 | Water washing device applied to washing edible mushroom blake bottle |
-
2012
- 2012-01-13 CN CN 201210009192 patent/CN103202174A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104756751A (en) * | 2014-01-07 | 2015-07-08 | 陕西省微生物研究所 | Edible fungi bag cultivation technology based on natural rain simulation |
| CN106688603A (en) * | 2015-08-11 | 2017-05-24 | 天津市庆祥农业科技有限公司 | Preparation method of edible mushrooms and process thereof |
| CN105900806A (en) * | 2016-04-22 | 2016-08-31 | 固镇县绿禾家庭农场 | Planting method for high-yield pollution-free cucumbers |
| CN107637382A (en) * | 2017-09-29 | 2018-01-30 | 永州市祥瑞生物科技有限公司 | Water washing device applied to washing edible mushroom blake bottle |
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Application publication date: 20130717 |