CN116769606A - Method for breeding and producing seeds of mushroom - Google Patents
Method for breeding and producing seeds of mushroom Download PDFInfo
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- CN116769606A CN116769606A CN202210221227.3A CN202210221227A CN116769606A CN 116769606 A CN116769606 A CN 116769606A CN 202210221227 A CN202210221227 A CN 202210221227A CN 116769606 A CN116769606 A CN 116769606A
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- mushroom
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000009395 breeding Methods 0.000 title claims abstract description 7
- 230000001488 breeding effect Effects 0.000 title claims abstract description 7
- 238000009423 ventilation Methods 0.000 claims abstract description 30
- 241000233866 Fungi Species 0.000 claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 12
- 238000011081 inoculation Methods 0.000 claims abstract description 7
- 238000007796 conventional method Methods 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 230000001902 propagating effect Effects 0.000 claims 3
- 230000035784 germination Effects 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 239000008187 granular material Substances 0.000 abstract 1
- 239000002245 particle Substances 0.000 description 14
- 239000007787 solid Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Mycology (AREA)
- Virology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method for breeding and producing seeds by mushroom propagation, which comprises the following steps: step one, conventionally preparing a culture medium; step two, respectively filling culture mediums into strain holes of a culture device and compacting; covering an air permeable cover; step four, sterilizing and cooling according to the conventional method; step five, opening an upper ventilation cover under the aseptic condition according to the conventional method, and inoculating strain holes respectively; step six, covering an upper ventilation cover and a lower ventilation cover after inoculation is finished, and culturing in a conventional mode; and step seven, culturing the strains, opening the ventilation cover in a sterile environment, taking out the strains in a whole, and inoculating in a fungus bag. The fungus seed producing process has the fungus holes in honeycomb body, and the fungus seed is cultured in separate chambers to form columnar fungus seed with volume greater than that of granule fungus seed, and the fungus seed has great amount of fungus mycelium, convenient production, less pollution, high quality, easy germination, easy mechanical operation and capacity of raising the fungus seed quality.
Description
Technical Field
The application relates to the field of microbial strain propagation.
Background
Microorganisms including bacteria, viruses, fungi, mushrooms, small protozoa, microalgae and the like are a large group of organisms, and the microorganisms are widely applied to various fields of foods, medicines, industry and agriculture, environmental protection and the like, so that many biologists in society are constantly devoted to culture, propagation and research of microorganisms.
The microbial strain propagation is a basic technology widely used in the fields of microbiology, biomedicine and microbiology, when the microbial strain is subjected to expansion culture, namely propagation, a culture medium for microbial culture is firstly selected, then the culture medium is inoculated and cultured, and finally the cultured strain is used for the expansion culture and propagation in quantity, for example, the mushroom propagation process is mother strain-stock strain-cultivated strain, the strain is generally divided into three stages, namely, the first stage is mother strain, the second stage is stock strain, the third stage is cultivated strain, the first stage to the third stage is for propagation of the strain, and the conventional mushroom propagation technology comprises the following steps: (1) In the whole propagation process, the strain is crushed and fine through multiple particle separation, so that hypha strain is caused, as shown in fig. 1 and 2, grain particles are inoculated and propagated into grain particle strain 1, a plurality of particle culture mediums are filled in a large container for culturing and seed production, hypha particles are taken out, and hypha strain is caused in the process of separating hypha particles, and the hypha strain is easy to pollute in multiple operations; (2) The mycelium growth culture medium is solid whole, such as ice cream stick and branch strain, which is not aerated, has few matrix mycelium and more aerial mycelium, resulting in poor mycelium quality, as shown in fig. 3 and 4, the amount of mycelium in solid wood is small, and mycelium grows on the surface and the germination is weak; (3) The method has high requirements and poor adaptability by adopting liquid strain propagation. In conclusion, the prior art results in time and labor consumption in the whole strain manufacturing, using and transporting processes, and the strain quality is poor. Therefore, a culture device and a culture method suitable for mushroom propagation are urgently needed at present, so that mushroom is time-saving and effective in the expansion propagation process, and has low pollution rate and high strain quality.
Disclosure of Invention
In order to solve the problems of the traditional mushroom propagation method, the application provides a mushroom propagation seed production method.
The technical scheme adopted by the application for achieving the purpose is as follows: a method for breeding and producing seeds by mushroom propagation, which comprises the following steps:
step one, conventionally preparing a culture medium;
step two, respectively filling culture mediums into strain holes 8 of a culture device and compacting;
covering an air permeable cover;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening an upper ventilation cover under the aseptic condition, and respectively inoculating the strain holes 8;
step six, covering an upper ventilation cover 4 and a lower ventilation cover 6 after inoculation is finished, and culturing in a conventional mode;
and step seven, culturing the strains, opening a ventilation cover in a sterile environment, taking out the strains 26 in a whole, and inoculating in the fungus bag 2.
In the first step, the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%.
In the seventh step, the propagation strain is in a basal column shape, and the specification of the propagation strain 25 is 3 x 18cm.
The culture device consists of a honeycomb body 5, an upper ventilation cover 4 and a lower ventilation cover 6, wherein a strain hole 8 which is vertically communicated is arranged in the honeycomb body 5.
The mushroom propagation seed production method of the application is characterized in that the mushroom holes are arranged in the honeycomb body, and the mushroom holes are integrally cultured in a chamber to form a basal column-shaped mushroom consisting of particles, the volume is larger than that of the particle mushroom, and the mushroom has the advantages of hard breaking of hypha in use, convenient production, hard pollution, high quality, easy germination, convenient mechanical operation and capability of improving the quality of the mushroom.
Drawings
FIG. 1 is a schematic diagram of conventional grain particle breeding.
FIG. 2 is a schematic diagram of a conventional grain seed strain planting.
Figure 3 is a schematic diagram of a prior art shoot breeding.
FIG. 4 is a schematic representation of a prior art shoot strain planting.
FIG. 5 is a front view showing an exploded view of the culture apparatus for propagation of mushroom according to the present application.
FIG. 6 is an external view showing the construction of the culture apparatus for propagation of mushroom according to the present application.
FIG. 7 is a schematic front view of a cross-sectional view of a culture apparatus for propagation of fungus according to the present application.
FIG. 8 is a schematic sectional view showing a state in which a culture apparatus for propagation of a fungus of the present application is packed in a medium.
FIG. 9 is a schematic cross-sectional view showing the propagation state of a culture apparatus for propagation of fungus according to the present application.
In the figure: 1. grain particle strain 2, fungus bag 3, branch strain 4, upper ventilation cover 5, honeycomb body 6, lower ventilation cover 7, clamping groove 8, strain hole 25, strain 26, basal column strain.
Detailed Description
As shown in FIG. 5, the culture device for mushroom propagation of the application comprises an upper ventilation cover 4, a honeycomb body 5 and a lower ventilation cover 6, wherein round strain holes 8 are vertically and longitudinally penetrated in the cylindrical honeycomb body 5, and the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged at the upper part and the lower part of the honeycomb body 5. The outside of the honeycomb body 5 is provided with a clamping groove 7, and the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged on the honeycomb body 5 through a convex clamp and the clamping groove 7.
The honeycomb body 5 is provided with a plurality of strain holes 8, and is cultivated by adopting an integrated chamber to form a basal column strain composed of particles, so that the strain is not easy to break hypha in use, and the strain has large hypha quantity, convenient manufacture, difficult pollution, high quality and easy germination.
The propagation seed production method comprises the following steps:
step one, conventionally preparing a culture medium, wherein the culture medium is a solid culture medium, the granularity is less than 1.5 x 1.5cm, and the water content is less than 80%;
step two, filling culture mediums into the strain holes 8 respectively, compacting, and synchronously inoculating the holes, as shown in fig. 6 and 7;
covering an upper ventilation cover 4 and a lower ventilation cover 6, and culturing in a synchronous sealing mode;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the upper ventilation cover 4 or/and the lower ventilation cover 6 under the aseptic condition, and inoculating the strain 25 in the strain holes 8 respectively, as shown in fig. 8;
step six, covering the upper ventilation cover 4 and the lower ventilation cover 6 after inoculation is finished, and culturing in a conventional manner, as shown in fig. 9;
step seven, culturing the strain, wherein the strain is in a basal column shape and has the specification of 3 x 18cm; opening the upper ventilation cover 4 or the lower ventilation cover 6 under the aseptic environment, taking out the whole cylindrical strain 26, and putting the strain into the fungus bag 2 for inoculation.
Culturing strain, uncapping under aseptic condition, taking out whole column-like strain 26 at any end of honeycomb body 5, directly inserting into corresponding fungus hole of material bag 1, or sectionally inserting column-like strain 26 composed of granular culture medium into corresponding fungus hole of material bag, sealing and culturing conventionally.
The strain propagated by the application is obviously superior to the liquid strain, the branch solid wood strain and the grain strain in the prior art in the eating time, the germination time and the full package time after inoculation, and the pollution rate is obviously lower than that of the prior art. At the same time, the disease resistance, stress resistance and adaptability are all improved.
Comparison table of germination time and pollution rate of fungus bags after inoculation
| Germination time | Time of filling bag | Pollution rate | |
| The application relates to a basal column-shaped particle strain | For 12 hours | 26 days | 0.05% |
| Liquid strain | 24 hours | For 30 days | 0.1% |
| Solid wood branch strain | For 30 hours | For 35 days | 1% |
| Grain particle strain | 36 hours | For 38 days | 1 % |
As shown in the table, the germination time and the bag filling time of the basal column particle strain are less than those of other comparative strain propagation modes, and the pollution rate is obviously lower than that of other propagation modes.
The present application has been described in terms of embodiments, and it will be appreciated by those of skill in the art that various changes can be made to the features and embodiments, or equivalents can be substituted, without departing from the spirit and scope of the application. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the application without departing from the essential scope thereof. Therefore, it is intended that the application not be limited to the particular embodiment disclosed, but that the application will include all embodiments falling within the scope of the appended claims.
Claims (4)
1. A method for breeding and producing seeds by mushroom is characterized in that: the method comprises the following steps:
step one, conventionally preparing a culture medium;
step two, respectively filling culture mediums into strain holes (8) of a culture device and compacting;
covering an air permeable cover;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the ventilation cover under the aseptic condition, and inoculating the strain holes (8) respectively;
step six, covering an air permeable cover after inoculation is finished, and culturing in a conventional mode;
step seven, culturing the strains, opening the ventilation cover in a sterile environment, taking out the strains (26) in a whole, and inoculating in the fungus bag (2).
2. The method for propagating and producing seeds of mushroom according to claim 1, wherein: in the first step, the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%.
3. The method for propagating and producing seeds of mushroom according to claim 1, wherein: in the seventh step, the propagation strain is in a basal column shape, and the specification of the propagation strain (25) is 3 x 18cm.
4. The method for propagating and producing seeds of mushroom according to claim 1, wherein: the culture device consists of a honeycomb body (5), an upper ventilation cover (4) and a lower ventilation cover (6), wherein a strain hole (8) which is vertically communicated is arranged in the honeycomb body (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210221227.3A CN116769606A (en) | 2022-03-09 | 2022-03-09 | Method for breeding and producing seeds of mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210221227.3A CN116769606A (en) | 2022-03-09 | 2022-03-09 | Method for breeding and producing seeds of mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116769606A true CN116769606A (en) | 2023-09-19 |
Family
ID=87993633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210221227.3A Pending CN116769606A (en) | 2022-03-09 | 2022-03-09 | Method for breeding and producing seeds of mushroom |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116769606A (en) |
-
2022
- 2022-03-09 CN CN202210221227.3A patent/CN116769606A/en active Pending
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