CN116769569A - Culture device and method for mushroom propagation - Google Patents
Culture device and method for mushroom propagation Download PDFInfo
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- CN116769569A CN116769569A CN202210221219.9A CN202210221219A CN116769569A CN 116769569 A CN116769569 A CN 116769569A CN 202210221219 A CN202210221219 A CN 202210221219A CN 116769569 A CN116769569 A CN 116769569A
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000009423 ventilation Methods 0.000 claims abstract description 73
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 3
- 238000007796 conventional method Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000002245 particle Substances 0.000 abstract description 15
- 241000233866 Fungi Species 0.000 abstract description 13
- 230000035784 germination Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 241001391944 Commicarpus scandens Species 0.000 abstract description 3
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
A culture device and method for fungus propagation comprises an upper ventilation cover, a honeycomb body and a lower ventilation cover, wherein strain holes are formed in the honeycomb body in a vertically penetrating manner, the upper ventilation cover and the lower ventilation cover are respectively arranged on the upper part and the lower part of the honeycomb body, and ventilation holes are formed in the upper ventilation cover and the lower ventilation cover. According to the culture device and method for mushroom propagation, the strain holes are formed in the honeycomb body, and the culture is carried out in an integrated chamber to form the basal column strain composed of particles, the volume is larger than that of the particle strain, hyphae are not easy to break in use, the strain hyphae are large in quantity, the production is convenient, pollution is not easy to occur, the quality is high, germination is easy, mechanical operation is convenient, and the strain quality can be improved; and the ventilation cover structure realizes the internal and external exchange of gas, ventilation is filtered, external miscellaneous bacteria cannot enter the inside, the ventilation effect is good, and the plug pulling is convenient.
Description
Technical Field
The application relates to the field of microbial strain propagation.
Background
Microorganisms including bacteria, viruses, fungi, mushrooms, small protozoa, microalgae and the like are a large group of organisms, and the microorganisms are widely applied to various fields of foods, medicines, industry and agriculture, environmental protection and the like, so that many biologists in society are constantly devoted to culture, propagation and research of microorganisms.
The microbial strain propagation is a basic technology widely used in the fields of microbiology, biomedicine and microbiology, when the microbial strain is subjected to expansion culture, namely propagation, a culture medium for microbial culture is firstly selected, then the culture medium is inoculated and cultured, and finally the cultured strain is used for the expansion culture and propagation in quantity, for example, the mushroom propagation process is mother strain-stock strain-cultivated strain, the strain is generally divided into three stages, namely, the first stage is mother strain, the second stage is stock strain, the third stage is cultivated strain, the first stage to the third stage is for propagation of the strain, and the conventional mushroom propagation technology comprises the following steps: (1) In the whole propagation process, the strain is crushed and fine through multiple particle separation, so that hypha strain is caused, as shown in fig. 1 and 2, grain particles are inoculated and propagated into grain particle strain 1, a plurality of particle culture mediums are filled in a large container for culturing and seed production, hypha particles are taken out, and hypha strain is caused in the process of separating hypha particles, and the hypha strain is easy to pollute in multiple operations; (2) The mycelium growth culture medium is solid whole, such as ice cream stick and branch strain, which is not aerated, has few matrix mycelium and more aerial mycelium, resulting in poor mycelium quality, as shown in fig. 3 and 4, the amount of mycelium in solid wood is small, and mycelium grows on the surface and the germination is weak; (3) The method has high requirements and poor adaptability by adopting liquid strain propagation. In conclusion, the prior art results in time and labor consumption in the whole strain manufacturing, using and transporting processes, and the strain quality is poor. Therefore, a culture device and a culture method suitable for mushroom propagation are urgently needed at present, so that mushroom is time-saving and effective in the expansion propagation process, and has low pollution rate and high strain quality.
In addition, microbial strain culture is a strict aseptic operation process, a plurality of particle culture mediums are usually filled in a large container for culturing and seed production, a vent plug is plugged into the seed production container, the existing vent plug is usually of a simple plug structure, the structure is not good in sealing and ventilation effects, and the plug is inconvenient to pull out, meanwhile, mechanical automation operation is not facilitated, the efficiency is low, the operation time is long, and pollution is easy.
Disclosure of Invention
In order to solve the problems of the traditional mushroom propagation method, the application provides a culture device and a method for mushroom propagation.
The technical scheme adopted by the application for achieving the purpose is as follows: a culture device for mushroom propagation comprises an upper ventilation cover 4, a honeycomb body 5 and a lower ventilation cover 6, wherein a strain hole 8 is formed in the honeycomb body 5 in a vertically penetrating manner, the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged at the upper part and the lower part of the honeycomb body 5, and ventilation holes are formed in the upper ventilation cover 4 and the lower ventilation cover 6.
The upper ventilation cover 4 and the lower ventilation cover 6 comprise an inner cavity 13 and an outer cavity 19, the inner cavity 13 is positioned at the center of the inner side of the outer cavity 19, and the bottom of the inner cavity 13 is communicated with the outer cavity 19; the outer cavity 19 comprises an outer cavity upper cover 9 and an outer cavity lower cover 21, a clamping seat 15 is arranged on the inner side of the outer cavity upper cover 9, the clamping seat 15 is positioned on the outer side of the inner cavity 13, and an outer cavity air hole 17 is formed in the outer edge 16 of the outer cavity upper cover 9 and positioned on the outer side of the clamping seat 15; the inner cavity upper cover 11 of the inner cavity 13 is arranged on the outer cavity upper cover 9 through the inner cavity brackets 14, an inner cavity transom 12 is formed between the inner cavity brackets 14, and a communication structure is arranged between the inner cavity 13 and the outer cavity 19.
Clamping edges 23 are respectively arranged on the outer sides of the upper part and the lower part of the honeycomb body 5, and clamping grooves 7 are formed in the upper part and the lower part of the upper clamping edge 23 and the lower clamping edge 23 which are positioned on the outer side of the honeycomb body 5; the inside of the clamping seat 15 is provided with a convex card 10 matched with the clamping groove 7, and the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged on the honeycomb body 5 through the convex card 10 and the clamping groove 7.
A filter disc 20 is arranged on the upper cover 9 of the outer cavity at the position communicated with the inner cavity 13.
Inside the clamping seat 15 is a tip-shaped slope 22.
A method for propagation of a fungus using the culture apparatus of claim 1, comprising the steps of:
step one, conventionally preparing a culture medium;
step two, filling culture mediums into the strain holes respectively and compacting;
step three, covering the upper ventilation cover 4 and the lower ventilation cover 6;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the upper ventilation cover 4 or/and the lower ventilation cover 6 under the aseptic condition, and inoculating the strain holes 8 respectively;
step six, covering an upper ventilation cover 4 and a lower ventilation cover 6 after inoculation is finished, and culturing in a conventional mode;
step seven, culturing the strains, opening the upper ventilation cover 4 or the lower ventilation cover 6 in a sterile environment, taking out the columnar strains 26, and putting the strains into the fungus bag 2 for inoculation.
In the first step, the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%.
According to the culture device and method for mushroom propagation, the strain holes are formed in the honeycomb body, and the culture is carried out in an integrated chamber to form the basal column strain composed of particles, the volume is larger than that of the particle strain, hyphae are not easy to break in use, the strain hyphae are large in quantity, the production is convenient, pollution is not easy to occur, the quality is high, germination is easy, mechanical operation is convenient, and the strain quality can be improved; and the ventilation cover structure realizes the internal and external exchange of gas, ventilation is filtered, external miscellaneous bacteria cannot enter the inside, the ventilation effect is good, and the plug pulling is convenient.
Drawings
FIG. 1 is a schematic diagram of conventional grain particle breeding.
FIG. 2 is a schematic diagram of a conventional grain seed strain planting.
Figure 3 is a schematic diagram of a prior art shoot breeding.
FIG. 4 is a schematic representation of a prior art shoot strain planting.
FIG. 5 is a front view showing an exploded view of the culture apparatus for propagation of mushroom according to the present application.
FIG. 6 is a schematic sectional exploded view of a culture apparatus for propagation of fungus according to the present application.
FIG. 7 is a schematic view of the aeration head of the culture apparatus for mushroom propagation according to the present application.
FIG. 8 is a schematic front view of a sectional view of a ventilation cover of the culture apparatus for propagation of fungus according to the present application.
FIG. 9 is a schematic diagram showing a plan view of a honeycomb structure of a culture apparatus for propagation of fungus according to the present application.
FIG. 10 is a schematic diagram showing a front view of a cross section of a cell body of the culture apparatus for mushroom propagation according to the present application.
Fig. 11 is an enlarged view of a portion a of fig. 10.
FIG. 12 is an external view showing the construction of the culture apparatus for propagation of mushroom according to the present application.
FIG. 13 is a schematic front view in cross section of a culture apparatus for propagation of mushroom according to the present application.
FIG. 14 is a schematic cross-sectional view showing a state in which a culture apparatus for propagation of a fungus of the present application is packed in a medium.
FIG. 15 is a schematic cross-sectional view showing the propagation state of a culture apparatus for propagation of fungus according to the present application.
FIG. 16 is a front view showing the structure of the culture apparatus according to the present application in a strain-removed state.
FIG. 17 is a schematic diagram showing the state of strain transportation.
FIG. 18 is a schematic diagram showing the state of the strain inserted into the fungus bag according to the first embodiment.
FIG. 19 is a schematic diagram showing the state of the second strain inserted into the bag.
In the figure: 1. grain particle strain 2, fungus bag 3, branch strain 4, upper ventilation cover 5, honeycomb body 6, lower ventilation cover 7, clamping groove 8, strain hole 9, outer cavity upper cover 10, convex clamp 11, inner cavity upper cover 12, inner cavity transom 13, inner cavity 14, inner cavity bracket 15, clamping seat 16, cover outer edge 17, outer cavity air hole 18, outer cavity upper cover outer bench 19, outer cavity 20, filter disc 21, outer cavity lower cover 22, tip slope 23, clamping edge 25, strain 26, base column strain 27, sealing bag.
Detailed Description
As shown in fig. 5 and 6, the culture device for mushroom propagation of the application comprises an upper ventilation cover 4, a honeycomb body 5 and a lower ventilation cover 6, wherein strain holes 8 are vertically and longitudinally arranged in the cylindrical honeycomb body 5, the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged at the upper part and the lower part of the honeycomb body 5, and ventilation holes are arranged on the upper ventilation cover 4 and the lower ventilation cover 6.
As shown in fig. 7 and 8, the upper ventilation cap 4 and the lower ventilation cap 6 comprise an inner cavity 13 and an outer cavity 19, the annular inner cavity 13 is positioned at the inner center of the outer cavity 19, and the bottom of the inner cavity 13 is communicated with the outer cavity 19; the outer cavity 19 comprises an outer cavity upper cover 9 and an outer cavity lower cover 21, an annular clamping seat 15 is arranged on the inner side of the outer cavity upper cover 9, the clamping seat 15 is positioned on the outer side of the inner cavity 13, the inner side of the outer cavity 19 is of a concave structure, the inner cavity 13 is positioned at a concave position, the outer side of the concave structure is an outer cavity upper cover outer table 18, an outer cavity air hole 17 is formed in the outer side of the clamping seat 15 on the cover outer edge 16 of the outer cavity upper cover 9, and the cover outer edge 16 protrudes out of the annular clamping seat 15; the inner cavity upper cover 11 of the inner cavity 13 is arranged on the outer cavity upper cover 9 through the inner cavity brackets 14, an inner cavity transom 12 is formed between the inner cavity brackets 14, a communication structure is arranged between the inner cavity 13 and the outer cavity 19, and a filter disc 20 is arranged on the outer cavity upper cover 9 at the position communicated with the inner cavity 13.
As shown in fig. 9-11, the upper and lower outer sides of the honeycomb body 5 are respectively provided with a clamping edge 23, and the upper and lower parts of the upper and lower clamping edges 23 on the outer side of the honeycomb body 5 are provided with clamping grooves 7; the inside of the clamping seat 15 is provided with a convex card 10 matched with the clamping groove 7, and the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged on the honeycomb body 5 through the convex card 10 and the clamping groove 7. The inner side of the clamping seat 15 is provided with a tip-shaped slope 22, the inlet direction of the annular clamping seat 15 is thin, and the convex clamp 10 with friction tensioning function is arranged, so that the sealing is facilitated, and the clamping and the pulling-in are facilitated.
The honeycomb body 5 is provided with a plurality of strain holes 8, and is cultivated by adopting an integrated chamber to form a basal column strain composed of particles, so that the strain is not easy to break hypha in use, and the strain has large hypha quantity, convenient manufacture, difficult pollution, high quality and easy germination.
The using method is as follows:
step one, conventionally preparing a culture medium, wherein the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%;
step two, filling culture mediums into the strain holes 8 respectively and compacting the culture mediums, as shown in fig. 12 and 13;
step three, the upper ventilation cover 4 and the lower ventilation cover 6 are covered, and air enters the strain holes 8 of the honeycomb body 5 for exchange through the outer cavity air holes 17, the filter discs 20 and the inner cavity air windows 12, so that the ventilation effect is good;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the upper ventilation cover 4 or/and the lower ventilation cover 6 under the aseptic condition, and inoculating the strain 25 in the strain holes 8 respectively, as shown in fig. 14;
step six, covering the upper ventilation cover 4 and the lower ventilation cover 6 after inoculation is finished, and culturing in a conventional manner, as shown in fig. 15;
step seven, culturing the strain, wherein the strain is in a basal column shape and has the specification of 3-18 cm; as shown in fig. 16, the upper ventilation cap 4 or the lower ventilation cap 6 is opened in a sterile environment, and the basal column strain 26 is taken out and placed in the fungus bag 2 for inoculation.
After culturing the strain, the lid is opened in a sterile environment, and the whole of the basal pillar-like strain 26 is taken out at either end of the honeycomb body 5, as shown in FIG. 16. The removed basal cylindrical strain 26 is directly packed into bags and sealed, and the sealed bags are packed into containers for storage or transportation, as shown in fig. 17. The aseptic package is inoculated in a conventional aseptic environment, and the basal column strains 26 consisting of the granular culture medium are directly inserted into corresponding bacterial holes of the package 1, as shown in fig. 18. Or sectionally inoculating the basal column strains 26 consisting of the granular culture medium into corresponding strain holes of the material packet, as shown in fig. 19. Sealing and culturing conventionally.
The strain propagated by the application is obviously superior to the liquid strain, the branch solid wood strain and the grain strain in the prior art in the eating time, the germination time and the full package time after inoculation, and the pollution rate is obviously lower than that of the prior art. At the same time, the disease resistance, stress resistance and adaptability are all improved.
Comparison table of germination time and pollution rate of fungus bags after inoculation
As shown in the table, the germination time and the bag filling time of the basal column particle strain are less than those of other comparative strain propagation modes, and the pollution rate is obviously lower than that of other propagation modes.
The present application has been described in terms of embodiments, and it will be appreciated by those of skill in the art that various changes can be made to the features and embodiments, or equivalents can be substituted, without departing from the spirit and scope of the application. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the application without departing from the essential scope thereof. Therefore, it is intended that the application not be limited to the particular embodiment disclosed, but that the application will include all embodiments falling within the scope of the appended claims.
Claims (7)
1. A culture apparatus for the propagation of mushrooms, characterized in that: the upper ventilation cover (4) and the lower ventilation cover (6) are respectively arranged at the upper part and the lower part of the honeycomb body (5), and ventilation holes are formed in the upper ventilation cover (4) and the lower ventilation cover (6) respectively.
2. A culture apparatus for the propagation of mushrooms according to claim 1, wherein: the upper ventilation cover (4) and the lower ventilation cover (6) comprise an inner cavity (13) and an outer cavity (19), the inner cavity (13) is positioned at the center of the inner side of the outer cavity (19), and the bottom of the inner cavity (13) is communicated with the outer cavity (19); the outer cavity (19) comprises an outer cavity upper cover (9) and an outer cavity lower cover (21), a clamping seat (15) is arranged on the inner side of the outer cavity upper cover (9), the clamping seat (15) is positioned on the outer side of the inner cavity (13), and an outer cavity air hole (17) is formed in the outer edge (16) of the outer cover (9) and positioned on the outer side of the clamping seat (15); an inner cavity upper cover (11) of the inner cavity (13) is arranged on the outer cavity upper cover (9) through an inner cavity bracket (14), an inner cavity transom (12) is formed between the inner cavity brackets (14), and a communication structure is arranged between the inner cavity (13) and the outer cavity (19).
3. A culture device for the propagation of mushrooms according to claim 2, characterized in that: clamping edges (23) are respectively arranged on the outer sides of the upper part and the lower part of the honeycomb body (5), and clamping grooves (7) are formed in the upper part and the lower part of the upper clamping edge (23) and the lower clamping edge (23) on the outer side of the honeycomb body (5); the inner side of the clamping seat (15) is provided with a convex card (10) matched with the clamping groove (7), and the upper ventilation cover (4) and the lower ventilation cover (6) are respectively arranged on the honeycomb body (5) through the convex card (10) and the clamping groove (7).
4. A culture device for the propagation of mushrooms according to claim 2, characterized in that: the filter disc (20) is arranged at the position, communicated with the inner cavity (13), of the outer cavity upper cover (9).
5. A culture device for the propagation of mushrooms according to claim 2, characterized in that: the inner side of the clamping seat (15) is provided with a tip-shaped slope (22).
6. A method for propagation of mushroom using the culture apparatus of claim 1, characterized in that: the method comprises the following steps:
step one, conventionally preparing a culture medium;
step two, filling culture mediums into the strain holes respectively and compacting;
step three, covering an upper ventilation cover (4) and a lower ventilation cover (6);
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the upper ventilation cover (4) or/and the lower ventilation cover (6) under the aseptic condition, and inoculating the strain holes (8) respectively;
step six, covering an upper ventilation cover (4) and a lower ventilation cover (6) after inoculation is finished, and culturing in a conventional mode;
step seven, culturing the strains, opening the upper ventilation cover (4) or the lower ventilation cover (6) in a sterile environment, taking out the basal column strain (26), and putting the basal column strain into the strain bag (2) for inoculation.
7. The method for propagation of mushroom using the culture apparatus as claimed in claim 1, wherein: in the first step, the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210221219.9A CN116769569A (en) | 2022-03-09 | 2022-03-09 | Culture device and method for mushroom propagation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210221219.9A CN116769569A (en) | 2022-03-09 | 2022-03-09 | Culture device and method for mushroom propagation |
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| Publication Number | Publication Date |
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| CN116769569A true CN116769569A (en) | 2023-09-19 |
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| CN202210221219.9A Pending CN116769569A (en) | 2022-03-09 | 2022-03-09 | Culture device and method for mushroom propagation |
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