CN116769604A - Method for propagating and storing mushroom - Google Patents
Method for propagating and storing mushroom Download PDFInfo
- Publication number
- CN116769604A CN116769604A CN202210221217.XA CN202210221217A CN116769604A CN 116769604 A CN116769604 A CN 116769604A CN 202210221217 A CN202210221217 A CN 202210221217A CN 116769604 A CN116769604 A CN 116769604A
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- Prior art keywords
- mushroom
- strain
- ventilation cover
- honeycomb body
- basal
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- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000001902 propagating effect Effects 0.000 title claims abstract description 7
- 238000009423 ventilation Methods 0.000 claims abstract description 26
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 238000003860 storage Methods 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000002245 particle Substances 0.000 abstract description 14
- 230000035784 germination Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 241001391944 Commicarpus scandens Species 0.000 abstract description 3
- 238000007796 conventional method Methods 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 description 6
- 238000012258 culturing Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- Mushroom Cultivation (AREA)
Abstract
A method for propagating and storing mushroom, comprising the following steps: step one, after strains in a culture medium are cultured, opening an upper ventilation cover or/and a lower ventilation cover of the honeycomb culture device under the aseptic condition according to the conventional method; step two, taking out the basal column strain from the whole strip at any end of the honeycomb body of the culture device; step three, filling the extracted basal column strains into a sealing bag and sealing; step four, placing the sealed bags with the seals into a container for storage or transportation; according to the mushroom propagation and storage method, the mushroom holes are formed in the honeycomb body, and the mushroom holes are integrally cultured in a chamber to form the basal column-shaped mushroom which is composed of particles, the volume is larger than that of the particle mushroom, the mushroom hyphae are not easy to break in use, the mushroom hyphae are large in quantity, convenient to manufacture and high in quality, germination is easy, mechanical operation is convenient, and the mushroom quality can be improved; and the sealed bags are used for storage or transportation, so that the time and the labor are saved, and the pollution is not easy to occur.
Description
Technical Field
The application relates to the field of microbial strain propagation.
Background
Microorganisms including bacteria, viruses, fungi, mushrooms, small protozoa, microalgae and the like are a large group of organisms, and the microorganisms are widely applied to various fields of foods, medicines, industry and agriculture, environmental protection and the like, so that many biologists in society are constantly devoted to culture, propagation and research of microorganisms.
The microbial strain propagation is a basic technology widely used in the fields of microbiology, biomedicine and microbiology, when the microbial strain is subjected to expansion culture, namely propagation, a culture medium for microbial culture is firstly selected, then the culture medium is inoculated and cultured, and finally the cultured strain is used for the expansion culture and propagation in quantity, for example, the mushroom propagation process is mother strain-stock strain-cultivated strain, the strain is generally divided into three stages, namely, the first stage is mother strain, the second stage is stock strain, the third stage is cultivated strain, the first stage to the third stage is for propagation of the strain, and the conventional mushroom propagation technology comprises the following steps: (1) In the whole propagation process, the strain is crushed and fine through multiple particle separation, so that hypha strain is caused, as shown in fig. 1 and 2, grain particles are inoculated and propagated into grain particle strain 1, a plurality of particle culture mediums are filled in a large container for culturing and seed production, hypha particles are taken out, and hypha strain is caused in the process of separating hypha particles, and the hypha strain is easy to pollute in multiple operations; (2) The mycelium growth culture medium is solid whole, such as ice cream stick and branch strain, which is not aerated, has few matrix mycelium and more aerial mycelium, resulting in poor mycelium quality, as shown in fig. 3 and 4, the amount of mycelium in solid wood is small, and mycelium grows on the surface and the germination is weak; (3) The method has high requirements and poor adaptability by adopting liquid strain propagation. In conclusion, the prior art results in time and labor consumption in the whole strain manufacturing, using and transporting processes, and the strain quality is poor.
Disclosure of Invention
In order to solve the problems of the traditional mushroom propagation method, the application provides a mushroom propagation and storage method.
The technical scheme adopted by the application for achieving the purpose is as follows: a method for propagating and storing mushroom, comprising the following steps:
step one, after strains in a culture medium are cultured, opening an upper ventilation cover 4 or/and a lower ventilation cover 6 of the honeycomb culture device under the aseptic condition conventionally;
step two, taking out the basal column bacterial strain 26 from any end of the honeycomb body 5 of the culture device;
step three, filling the extracted basal column strains 26 into a sealing bag 27 and sealing;
step four, placing the sealed bag 27 with the seal into a container for storage or transportation;
in the second step, the granularity of the culture medium is less than 1.5-1.5 cm, and the water content is less than 80%.
The culture device consists of a honeycomb body 5, an upper ventilation cover 4 and a lower ventilation cover 6, wherein a strain hole 8 which is vertically communicated is arranged in the honeycomb body 5.
The specification of the basal columnar strain 26 is 3 x 18cm.
According to the mushroom propagation and storage method, the mushroom holes are formed in the honeycomb body, and the mushroom holes are integrally cultured in a chamber to form the basal column-shaped mushroom which is composed of particles, the volume is larger than that of the particle mushroom, the mushroom hyphae are not easy to break in use, the mushroom hyphae are large in quantity, convenient to manufacture and high in quality, germination is easy, mechanical operation is convenient, and the mushroom quality can be improved; and the sealed bags are used for storage or transportation, so that the time and the labor are saved, and the pollution is not easy to occur.
Drawings
FIG. 1 is a schematic diagram of conventional grain particle breeding.
FIG. 2 is a schematic diagram of a conventional grain seed strain planting.
Figure 3 is a schematic diagram of a prior art shoot breeding.
FIG. 4 is a schematic representation of a prior art shoot strain planting.
FIG. 5 is a front view showing an exploded view of the culture apparatus for propagation of mushroom according to the present application.
FIG. 6 is a schematic sectional exploded view of a culture apparatus for propagation of fungus according to the present application.
FIG. 7 is a schematic cross-sectional view showing the propagation state of a culture apparatus for propagation of fungus according to the present application.
FIG. 8 is a front view showing the structure of the culture apparatus according to the present application in a strain-removed state.
FIG. 9 is a schematic diagram showing the state of strain transportation.
In the figure: 1. grain particle strain 2, fungus bag 3, branch strain 4, upper ventilation cover 5, honeycomb body 6, lower ventilation cover 7, clamping groove 26, basal column strain 27, and sealing bag.
Detailed Description
As shown in fig. 5 and 6, the culture device for mushroom propagation of the application comprises an upper ventilation cover 4, a honeycomb body 5 and a lower ventilation cover 6, wherein strain holes 8 are vertically and longitudinally penetrated in the cylindrical honeycomb body 5, the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged at the upper part and the lower part of the honeycomb body 5, a clamping groove 7 is arranged at the outer part of the honeycomb body, and the upper ventilation cover 4 and the lower ventilation cover 6 are respectively arranged on the honeycomb body 5 through convex clamps and the clamping groove 7.
The honeycomb body 5 is provided with a plurality of strain holes 8, and is cultivated by adopting an integrated chamber to form a basal column strain composed of particles, so that the strain is not easy to break hypha in use, and the strain has large hypha quantity, convenient manufacture, difficult pollution, high quality and easy germination.
The using method is as follows:
step one, conventionally preparing a culture medium, wherein the granularity of the culture medium is less than 1.5 x 1.5cm, and the water content is less than 80%;
filling culture mediums into the strain holes 8 respectively and compacting;
step three, covering the upper ventilation cover 4 and the lower ventilation cover 6;
step four, sterilizing and cooling according to the conventional method;
step five, conventionally opening the upper ventilation cover 4 or/and the lower ventilation cover 6 under the aseptic condition, and inoculating strains in the strain holes 8 respectively;
step six, covering the upper ventilation cover 4 and the lower ventilation cover 6 after inoculation is finished, and culturing in a conventional manner, as shown in fig. 7;
step seven, culturing the strain, wherein the strain is in a basal column shape and has the specification of 3-18 cm; as shown in fig. 8, the upper ventilation cap 4 or the lower ventilation cap 6 is opened in a sterile environment, and the basal column strain 26 is taken out and placed in the fungus bag 2 for inoculation.
Culturing strain, opening the cover under aseptic condition, taking out the whole piece of basal column strain 26 at either end of honeycomb body 5, directly bagging the basal column strain 26, sealing, and storing or transporting the sealed bag in a container, as shown in figure 9.
The strain propagated by the application is obviously superior to the liquid strain, the branch solid wood strain and the grain strain in the prior art in the eating time, the germination time and the full package time after inoculation, and the pollution rate is obviously lower than that of the prior art. At the same time, the disease resistance, stress resistance and adaptability are all improved.
Comparison table of germination time and pollution rate of fungus bags after inoculation
As shown in the table, the germination time and the bag filling time of the basal column particle strain are less than those of other comparative strain propagation modes, and the pollution rate is obviously lower than that of other propagation modes.
The present application has been described in terms of embodiments, and it will be appreciated by those of skill in the art that various changes can be made to the features and embodiments, or equivalents can be substituted, without departing from the spirit and scope of the application. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the application without departing from the essential scope thereof. Therefore, it is intended that the application not be limited to the particular embodiment disclosed, but that the application will include all embodiments falling within the scope of the appended claims.
Claims (4)
1. A method for propagating and storing mushroom, which is characterized in that: the method comprises the following steps:
step one, after strains in a culture medium are cultured, opening an upper ventilation cover (4) or/and a lower ventilation cover (6) of the honeycomb culture device under the aseptic condition conventionally;
step two, taking out the columnar basal strain (26) at any end of the honeycomb body (5) of the culture device;
step three, filling the extracted basal column strains (26) into a sealing bag (27) and sealing;
and step four, placing the sealed bag (27) with the seal into a container for storage or transportation.
2. A method for propagation and storage of mushroom according to claim 1, characterized in that: in the second step, the granularity of the culture medium is less than 1.5-1.5 cm, and the water content is less than 80%.
3. The method for propagating and producing seeds of mushroom according to claim 1, wherein the method comprises the following steps: the culture device consists of a honeycomb body (5), an upper ventilation cover (4) and a lower ventilation cover (6), wherein a strain hole (8) which is vertically communicated is arranged in the honeycomb body (5).
4. The method for propagating and producing seeds of mushroom according to claim 1, wherein the method comprises the following steps: the specification of the basal columnar strain (26) is 3 x 18cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210221217.XA CN116769604A (en) | 2022-03-09 | 2022-03-09 | Method for propagating and storing mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210221217.XA CN116769604A (en) | 2022-03-09 | 2022-03-09 | Method for propagating and storing mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116769604A true CN116769604A (en) | 2023-09-19 |
Family
ID=88012121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210221217.XA Pending CN116769604A (en) | 2022-03-09 | 2022-03-09 | Method for propagating and storing mushroom |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116769604A (en) |
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2022
- 2022-03-09 CN CN202210221217.XA patent/CN116769604A/en active Pending
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