CN101250489A - Industrial method for artificially cultivating Chinese caterpillar fungus - Google Patents
Industrial method for artificially cultivating Chinese caterpillar fungus Download PDFInfo
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- CN101250489A CN101250489A CNA2008100546797A CN200810054679A CN101250489A CN 101250489 A CN101250489 A CN 101250489A CN A2008100546797 A CNA2008100546797 A CN A2008100546797A CN 200810054679 A CN200810054679 A CN 200810054679A CN 101250489 A CN101250489 A CN 101250489A
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Abstract
The invention relates to the technical field of culturing aweto and in particular relates to a method for culturing the aweto with artificial industrialization, which solves the problems of existing aweto industrialization culture that the investment is large, the cost is high, mycelium can not be separated out, and strains are easily degenerated. The method of the invention carries out two-grade seed production, namely, production in a test tube and the production in liquid. The mycelium and stroma are respectively cultured on artificial media, the research about the quality and the output of two shapes of the aweto is paid more attention, the culture of compound aweto is not emphasized, and effective components of the mycelium and the stroma are equivalent to that of natural aweto. The method of the invention is convenient and practical, raw materials can be easily obtained on the spot, the strains are not degenerated, the output is high, the quality is high, the cost is extremely low, and the method can be accepted by vast farmers.
Description
Technical field
The invention belongs to the technical field that Cordyceps sinensis is cultivated, be specifically related to the method that Cordyceps sinensis is cultivated in a kind of artificial industrialization.
Background technology
Since the eighties, relevant expert and fungi worker, from natural cs, isolate a plurality of cordyceps species (as cephalo, the bundle spore, by spore, Isaria or the like), and bacterial classification received on the bat moth larvae body or on the artificial medium, can both grow Chinese caterpillar fungus stroma (larva body and stroma are called complex body) on the larva body He on the substratum, all the effective constituent with natural cs is suitable for the effective constituent of its polypide mycelia and stroma after testing, and some composition is higher than natural, proved the alternative natural cs of artificial aweto with this, yet in past more than 20 year, artificial compound Chinese caterpillar fungus still rests on the laboratory.Some enterprise is the Cordyceps mycelium production development mass-producing with the bulk fermentation pattern, but investment is big, cost is high, can not separate mycelium, bacterial classification is easily degenerated.
Summary of the invention
The problem that the present invention cultivates in order to solve existing Cordyceps sinensis industrialization that investment is big, cost is high, can separates mycelium, bacterial classification is easily degenerated provides a kind of method of artificial industrialization cultivation Cordyceps sinensis.
The present invention adopts following technical scheme to realize:
The method of Cordyceps sinensis is cultivated in artificial industrialization, and its step is as follows:
1) cultivation of test tube kind
1.1), the prescription: analysis for soybean powder 30～50%, Semen Maydis grit 10～30%, small rice grain 5～15%, sorghum flour 5～15%, fresh hen egg 10～30%;
1.2), method: the material of getting above-mentioned weight ratio, sorghum flour, small rice grain, Semen Maydis grit are soaked in water to the no dried heart, pull out then and admix analysis for soybean powder, fresh hen egg, add water then, the weight of water is 60～65% of all material gross weights, 1/3 of the full test tube of the test tube of packing into then, conventional disinfection inoculation gets the test tube kind;
2) cultivation of liquid strain
The test tube kind is inserted the mixture of forming by analysis for soybean powder, Semen Maydis powder, sorghum flour, analysis for soybean powder, Semen Maydis powder, sorghum flour respectively are about 1/3 in the mixture, the weight that connects the test tube kind is 10～20% of mixture weight, 18～25 ℃ of shake-flask culture in water distribution sterilization back;
3) culture medium for cultivating
3.1) mycelial cultivation
Liquid strain is inserted by Semen Maydis grit 30～50%, analysis for soybean powder 20～30%, wheat groat 10～20%, the mixture that sorghum grain 10～20% is formed, liquid strain are 15～20%, 20～25 ℃ of lucifuge static cultivation 15～20 days of mixture, mycelium,
3.2) cultivation of stroma
Liquid strain is inserted by Semen Maydis grit 30～50% analysis for soybean powder 30～50%, wheat groat 10～20%, sorghum grain 5～10%, the mixture that Ovum Gallus domesticus album 5～10% is formed, liquid strain are that 15～20%, 18～25 ℃ of lucifuges of mixture were cultivated about 15 days, later be astigmatism, totally 50 days, get stroma;
4), mycelium and stroma are combined into Cordyceps sinensis.
Cultivate the standby test tube kind of mycelium through 36 ℃ 48 hours, 20～25 ℃ 48 hours ,-30 ℃ 24 hours, 20～25 ℃ 24 hours, in the assorted collarium border 72 hours, then 20～25 ℃ of cultivations.
Cultivate the standby test tube kind of stroma and will often adhere to sexual propagation.
All materials are all-natural product.
The contrast of the effective constituent of the Cordyceps sinensis that the method for the invention obtains and natural cs effective constituent is checked as following table through China Medicine University.
| Sample | Qinghai-Tibet Golog natural cs | The Nanjing natural cs | The present invention |
| Qualification result | Cordyceps sinensis(berk.)Sacc | C.militarise(L.)link | C.sinensis(berk.)Sace |
| Crude polysaccharides content (%) | 60.78 | 31.64 | 50.03 |
| Cordycepin content (mg/g) | 0.03 | 0.04 | 0.82 |
| Adenosine content (mg/g) | 0.48 | 0.82 | 1.79 |
| Cordycepic acid content (mg/g) | 13.8 | 14.7 | 32.6 |
Method of the present invention was easy to learn, and the grower just can grasp contents such as preparation substratum, inoculation method, cultural method and results back segment time preservation substantially through 2～3 day time.The present invention carries out the two-stage production of hybrid seeds: test tube kind and liquid strain.The unification of test tube kind is prepared as liquid bacterium bag, and without aseptic technique, directly use gets final product in grower's hand.The container of dress substratum can be Cans or polyethylene bag, usually loading amount 50 grams.Sterilization is normal-pressure sterilization 10 hours.
The present invention has following beneficial effect: use the canister planting type, mycelium and stroma are cultivated on artificial medium respectively, pay attention to the quality of two kinds of forms of Chinese caterpillar fungus and the research of output, do not emphasize the cultivation of compound Chinese caterpillar fungus, the effective constituent of mycelium and stroma is all suitable with natural cs.The inventive method is simple and easy to do, and raw material is easy to get and gathers materials on the spot, and bacterial classification is not degenerated, and output height, quality good (mycelia can grow 2～3cm height, can separate with substratum), cost are extremely low, is as easy as rolling off a logly accepted by vast farmers.
Embodiment
Embodiment is used for illustrating of the present invention, rather than it is imposed any restrictions.
Embodiment 1:
The method of Cordyceps sinensis is cultivated in artificial industrialization, and its step is as follows:
1) cultivation of test tube kind
1.1), the prescription: analysis for soybean powder 30%, Semen Maydis grit 30%, small rice grain 10%, sorghum flour 10%, fresh hen egg 20%;
1.2), method: the material of getting above-mentioned weight ratio, sorghum flour, small rice grain, Semen Maydis grit are soaked in water to the no dried heart, pull out then and admix analysis for soybean powder, fresh hen egg, add water then, the weight of water is 60% of all material gross weights, 1/3 of the full test tube of the test tube of packing into then, conventional disinfection inoculation gets the test tube kind;
2) cultivation of liquid strain
The test tube kind is inserted the mixture of being made up of analysis for soybean powder, Semen Maydis powder, sorghum flour, and analysis for soybean powder, Semen Maydis powder, sorghum flour are about 1/3 respectively in the mixture, and the weight that connects the test tube kind is 15% of mixture weight, 18～25 ℃ of shake-flask culture in water distribution sterilization back;
3) culture medium for cultivating
3.1) mycelial cultivation
Liquid strain is inserted by Semen Maydis grit 30%, analysis for soybean powder 30%, wheat groat 20%, the mixture that sorghum grain 20% is formed, liquid strain are 15%, 20～25 ℃ of lucifuge static cultivation 15～20 days of mixture, mycelium,
3.2) cultivation of stroma
Liquid strain is inserted by Semen Maydis grit 50%, analysis for soybean powder 30%, wheat groat 10%, sorghum grain 5%, the mixture that Ovum Gallus domesticus album 5% is formed, liquid strain are that 20%, 18～25 ℃ of lucifuges of mixture were cultivated about 15 days, be later astigmatism, totally 50 days, get stroma;
4), mycelium and stroma are combined into Cordyceps sinensis.
The standby test tube kind of above-mentioned cultivation mycelium through 36 ℃ 48 hours, 20～25 ℃ 48 hours ,-30 ℃ 24 hours, 20～25 ℃ 24 hours, in the assorted collarium border 72 hours, standby 20～25 ℃ of cultivations then.
The standby test tube kind of above-mentioned cultivation stroma will often be adhered to sexual propagation.
Above-mentioned all materials are all-natural product.
Embodiment 2:
The method of Cordyceps sinensis is cultivated in artificial industrialization, and its step is as follows:
1) cultivation of test tube kind
1.1), the prescription: analysis for soybean powder 50%, Semen Maydis grit 10%, small rice grain 15%, sorghum flour 15%, fresh hen egg 10%;
1.2), method: the material of getting above-mentioned weight ratio, sorghum flour, small rice grain, Semen Maydis grit are soaked in water to the no dried heart, pull out then and admix analysis for soybean powder, fresh hen egg, add water then, the weight of water is 65% of all material gross weights, 1/3 of the full test tube of the test tube of packing into then, conventional disinfection inoculation gets the test tube kind;
2) cultivation of liquid strain
The test tube kind is inserted the mixture of being made up of analysis for soybean powder, Semen Maydis powder, sorghum flour, and analysis for soybean powder, Semen Maydis powder, sorghum flour are about 1/3 respectively in the mixture, and the weight that connects the test tube kind is 20% of mixture weight, 18～25 ℃ of shake-flask culture in water distribution sterilization back;
3) culture medium for cultivating
3.1) mycelial cultivation
Liquid strain is inserted by Semen Maydis grit 50%, analysis for soybean powder 20%, wheat groat 10%, the mixture that sorghum grain 20% is formed, liquid strain are 20%, 20～25 ℃ of lucifuge static cultivation 15～20 days of mixture, mycelium,
3.2) cultivation of stroma
Liquid strain is inserted by Semen Maydis grit 30%, analysis for soybean powder 40%, wheat groat 15%, sorghum grain 10%, the mixture that Ovum Gallus domesticus album 5% is formed, liquid strain are that 18%, 18～25 ℃ of lucifuges of mixture were cultivated about 15 days, be later astigmatism, totally 50 days, get stroma;
4), mycelium and stroma are combined into Cordyceps sinensis.
The standby test tube kind of above-mentioned cultivation mycelium through 36 ℃ 48 hours, 20～25 ℃ 48 hours ,-30 ℃ 24 hours, 20～25 ℃ 24 hours, in the assorted collarium border 72 hours, standby 20～25 ℃ of cultivations then.
The standby test tube kind of above-mentioned cultivation stroma will often be adhered to sexual propagation.
Above-mentioned all materials are all-natural product.
Embodiment 3:
The method of Cordyceps sinensis is cultivated in artificial industrialization, and its step is as follows:
1) cultivation of test tube kind
1.1), the prescription: analysis for soybean powder 40%, Semen Maydis grit 20%, small rice grain 5%, sorghum flour 5%, fresh hen egg 30%;
1.2), method: the material of getting above-mentioned weight ratio, sorghum flour, small rice grain, Semen Maydis grit are soaked in water to the no dried heart, pull out then and admix analysis for soybean powder, fresh hen egg, add water then, the weight of water is 62% of all material gross weights, 1/3 of the full test tube of the test tube of packing into then, conventional disinfection inoculation gets the test tube kind;
2) cultivation of liquid strain
The test tube kind is inserted the mixture of being made up of analysis for soybean powder, Semen Maydis powder, sorghum flour, and analysis for soybean powder, Semen Maydis powder, sorghum flour are about 1/3 respectively in the mixture, and the weight that connects the test tube kind is 10% of mixture weight, 18～25 ℃ of shake-flask culture in water distribution sterilization back;
3) culture medium for cultivating
3.1) mycelial cultivation
Liquid strain is inserted by Semen Maydis grit 50%, analysis for soybean powder 25%, wheat groat 15%, the mixture that sorghum grain 10% is formed, liquid strain are 18%, 20～25 ℃ of lucifuge static cultivation 15～20 days of mixture, mycelium,
3.2) cultivation of stroma
Liquid strain is inserted by Semen Maydis grit 30%, analysis for soybean powder 50%, wheat groat 10%, sorghum grain 5%, the mixture that Ovum Gallus domesticus album 10% is formed, liquid strain are that 15%, 18～25 ℃ of lucifuges of mixture were cultivated about 15 days, be later astigmatism, totally 50 days, get stroma;
4), mycelium and stroma are combined into Cordyceps sinensis.
The standby test tube kind of above-mentioned cultivation mycelium through 36 ℃ 48 hours, 20～25 ℃ 48 hours ,-30 ℃ 24 hours, 20～25 ℃ 24 hours, in the assorted collarium border 72 hours, standby 20～25 ℃ of cultivations then.
The standby test tube kind of above-mentioned cultivation stroma will often be adhered to sexual propagation.
Above-mentioned all materials are all-natural product.
Claims (4)
1, a kind of method of artificial industrialization cultivation Cordyceps sinensis, its step is as follows:
1) cultivation of test tube kind
1.1), the prescription: analysis for soybean powder 30～50%, Semen Maydis grit 10～30%, small rice grain 5～15%, sorghum flour 5～15%, fresh hen egg 10～30%;
1.2), method: the material of getting above-mentioned weight ratio, sorghum flour, small rice grain, Semen Maydis grit are soaked in water to the no dried heart, pull out then and admix analysis for soybean powder, fresh hen egg, add water then, the weight of water is 60～65% of all material gross weights, 1/3 of the full test tube of the test tube of packing into then, conventional disinfection inoculation gets the test tube kind;
2) cultivation of liquid strain
The test tube kind is inserted the mixture of forming by analysis for soybean powder, Semen Maydis powder, sorghum flour, analysis for soybean powder, Semen Maydis powder, sorghum flour respectively are about 1/3 in the mixture, the weight that connects the test tube kind is 10～20% of mixture weight, 18～25 ℃ of shake-flask culture in water distribution sterilization back;
3) culture medium for cultivating
3.1) mycelial cultivation
Liquid strain is inserted by Semen Maydis grit 30～50%, analysis for soybean powder 20～30%, wheat groat 10～20%, the mixture that sorghum grain 10～20% is formed, liquid strain are 15～20%, 20～25 ℃ of lucifuge static cultivation 15～20 days of mixture, mycelium,
3.2) cultivation of stroma
Liquid strain is inserted by Semen Maydis grit 30～50% analysis for soybean powder 30～50%, wheat groat 10～20%, sorghum grain 5～10%, the mixture that Ovum Gallus domesticus album 5～10% is formed, liquid strain are that 15～20%, 18～25 ℃ of lucifuges of mixture were cultivated about 15 days, later be astigmatism, totally 50 days, get stroma;
4), mycelium and stroma are combined into Cordyceps sinensis.
2, the method for Cordyceps sinensis is cultivated in artificial industrialization according to claim 1, it is characterized in that cultivating the standby test tube kind of mycelium through 36 ℃ 48 hours, 20～25 ℃ 48 hours,-30 ℃ 24 hours, 20～25 ℃ 24 hours, in the assorted collarium border 72 hours, then 20～25 ℃ of cultivations.
3, the method for artificial industrialization cultivation Cordyceps sinensis according to claim 1 is characterized in that cultivating used test tube kind as stroma will often adhere to sexual propagation.
4, according to the method for claim 1 or 2 or 3 described artificial industrialization cultivation Cordyceps sinensis, it is characterized in that above-mentioned all materials are all-natural product.
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| CNA2008100546797A CN101250489A (en) | 2008-03-22 | 2008-03-22 | Industrial method for artificially cultivating Chinese caterpillar fungus |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440141A (en) * | 2010-10-08 | 2012-05-09 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN102747027A (en) * | 2012-07-31 | 2012-10-24 | 福建农林大学 | Culture medium for producing large quantities of spores of aschersonia placenta |
| CN102870600A (en) * | 2012-10-17 | 2013-01-16 | 山西万海澳生物科技有限责任公司 | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content |
| CN103283478A (en) * | 2012-02-24 | 2013-09-11 | 北京市弘科农场 | Cordyceps militaris producing method |
| CN106171529A (en) * | 2016-08-05 | 2016-12-07 | 吴剑翔 | The Cordyceps cultural method of worm summer herb with high-content of cordycepin and the culture medium of use thereof |
| CN109496697A (en) * | 2018-11-28 | 2019-03-22 | 湖南金芙农业科技有限公司 | Swan bacterium spore separation and cultural method |
-
2008
- 2008-03-22 CN CNA2008100546797A patent/CN101250489A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440141A (en) * | 2010-10-08 | 2012-05-09 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN102440141B (en) * | 2010-10-08 | 2012-12-26 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN103283478A (en) * | 2012-02-24 | 2013-09-11 | 北京市弘科农场 | Cordyceps militaris producing method |
| CN103283478B (en) * | 2012-02-24 | 2014-10-29 | 北京市弘科农业科技服务站 | Cordyceps militaris producing method |
| CN102747027A (en) * | 2012-07-31 | 2012-10-24 | 福建农林大学 | Culture medium for producing large quantities of spores of aschersonia placenta |
| CN102747027B (en) * | 2012-07-31 | 2014-11-19 | 福建农林大学 | Culture medium for producing large quantities of spores of aschersonia placenta |
| CN102870600A (en) * | 2012-10-17 | 2013-01-16 | 山西万海澳生物科技有限责任公司 | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content |
| CN106171529A (en) * | 2016-08-05 | 2016-12-07 | 吴剑翔 | The Cordyceps cultural method of worm summer herb with high-content of cordycepin and the culture medium of use thereof |
| CN109496697A (en) * | 2018-11-28 | 2019-03-22 | 湖南金芙农业科技有限公司 | Swan bacterium spore separation and cultural method |
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Open date: 20080827 |