CN116669557A - 微生物群落中微生物的控制 - Google Patents

Abstract

描述了在微生物群落中产生分泌的抗微生物肽的方法。所述方法可以包括将核酸原位施用至所述微生物群落的至少一种期望的微生物有机体。遗传修饰的期望的微生物有机体分泌抗微生物肽。描述了微生物群落。

Description

微生物群落中微生物的控制

参考相关申请

本申请要求2020年11月10日提交的美国临时申请第63/112084号的权益。该相关申请的全部内容通过引用并入本文。参考序列表、表或计算机程序列表

本申请与电子格式的序列表一起提交。序列表以名称为SYNG007WOSEQUENCE.TXT的文件提供，创建并最后保存于2021年10月27日，大小为402,812个字节。序列表的电子格式的信息通过引用以其整体并入本文。

背景

微生物有机体如细菌可影响人类和动物健康，并参与与多种动物器官和组织相关的微生物群。微生物有机体介导的过程可用于多种工业过程中以生产目的产物，例如用于原料中的发酵。另外，微生物有机体可用于在无菌环境中制造产品，如在药物、生物制剂和化妆品的制造中。

调节微生物有机体的种群，例如以减少或消除不期望的微生物有机体，对于维持工业过程和维持包含微生物有机体的组织的健康是有用的。抗微生物肽如细菌素可影响微生物有机体的生长或活力。

领域

一些实施方案涉及在微生物群落中生产抗微生物肽。可以将编码抗微生物肽的核酸原位施用至微生物群落中的微生物有机体，如微生物组。

概述

在一些实施方案中，描述了在微生物群落中原位生产分泌的抗微生物肽的方法(为了简明起见，也称为“生产方法”)。生产方法可以包括将期望的微生物有机体鉴定为微生物群落的成员。生产方法可以包括将核酸原位施用至微生物群落的至少一种期望的微生物有机体，其中核酸编码抗微生物肽，所述抗微生物肽不杀死所鉴定的期望的微生物有机体或不阻止所鉴定的期望的微生物有机体的繁殖。因此，至少一种期望的微生物有机体被遗传修饰以表达抗微生物肽。生产方法还可以包括允许遗传修饰的期望的微生物有机体在微生物群落中生长，由此遗传修饰的期望的微生物有机体分泌抗微生物肽。在一些实施方案的生产方法中，从所述施用到所述允许遗传修饰的期望的微生物有机体生长，所述期望的微生物有机体彼此之间的比例基本上保持不变。在一些实施方案的生产方法中，没有将编码抗微生物肽的免疫调节剂的核酸施用至所述至少一种期望的微生物有机体。在一些实施方案的生产方法中，在所述分泌之前，所述微生物群落被一种或多种未知身份的不期望的微生物有机体污染。在一些实施方案的生产方法中，抗微生物肽杀死所述一种或多种不期望的微生物有机体或者阻止所述一种或多种不期望的微生物有机体的繁殖。在一些实施方案的生产方法中，抗微生物肽不被所述遗传修饰的期望的微生物有机体物种的野生型基因组编码。在一些实施方案的生产方法中，所述抗微生物肽是所述遗传修饰的微生物有机体外源的，或者其中所述抗微生物肽是合成的。在一些实施方案的生产方法中，所述核酸编码两种或更多种不同的抗微生物肽，因此所述遗传修饰的期望微生物细胞表达两种或更多种不同的抗微生物肽。在一些实施方案的生产方法中，施用所述核酸包括施用编码不同抗微生物肽的两种或更多种不同的核酸。在一些实施方案的生产方法中，一起选择所述不同的抗微生物肽以靶向抗生素抗性感染。在一些实施方案的生产方法中，施用所述核酸包括施用包含所述核酸的质粒；和/或施用包含所述核酸的噬菌体。在一些实施方案的生产方法中，期望的微生物有机体包括微生物有机体的两种或更多种不同物种，其中将所述核酸施用至所述不同物种中的仅一种或多于一种。在一些实施方案中，生产方法还包括将不同的核酸原位施用至所述微生物群落中不同于所述基因工程化的微生物有机体的微生物有机体，并且所述不同的核酸编码与所述核酸不同的抗微生物肽(因此，所述不同的微生物有机体被遗传修饰以表达所述不同的抗微生物肽)。在一些实施方案的生产方法中，所述不同的核酸与所述核酸同时施用。在一些实施方案的生产方法中，在与所述核酸不同的时间施用所述不同的核酸。在一些实施方案的生产方法中，所述微生物群落由微生物组组成。例如，所述微生物组可以是选自以下的微生物组：胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤，或者两种或更多种所列项目的组合。例如，可以将核酸施用至对象体内期望的微生物细胞。在一些实施方案的生产方法中，微生物群落是对象自体的，并且离体施用所述核酸。所述方法还可以包括将包含所述遗传修饰的期望的微生物有机体的微生物群落施用至所述对象。例如，微生物群落可以使对象的组织或器官的微生物组聚居(populate)或再聚居(repopulate)。在一些实施方案的生产方法中，所述微生物群落是保存的健康样品，并且其中在施用时，所述对象患有所述对象的微生物组的失调。在一些实施方案的生产方法中，所述微生物群落由工业培养物组成。

在一些实施方案中，描述了微生物群落。所述微生物群落可以包含期望的微生物有机体，其中所述期望的微生物有机体中的至少一种是遗传修饰的。所述遗传修饰的期望的微生物有机体可以包含编码不靶向期望的微生物有机体的第一抗微生物肽的第一核酸。因此，所述遗传修饰的期望微生物可经配置以表达第一抗微生物肽。所述第一核酸还可以编码所述第一抗微生物肽框内的分泌信号，例如本文所述，使得所述抗微生物肽在表达时可包含分泌信号。所述微生物群落还可以包含与所述第一核酸具有相同序列的无细胞的第二核酸。无细胞的第二核酸可以指示，例如，将第一核酸原位施用至遗传修饰的期望的微生物有机体。在一些实施方案的微生物群落中，所述第一抗微生物肽不被所述遗传修饰的期望的微生物有机体物种的野生型基因组编码。在一些实施方案的微生物群落中，所述第一抗微生物肽是合成的。在一些实施方案的微生物群落中，所述第一抗微生物肽是遗传修饰的微生物有机体外源的。在一些实施方案的微生物群落中，所述期望的微生物有机体不包含所述抗微生物肽的免疫调节剂。在一些实施方案的微生物群落中，微生物群落被一种或多种未知身份的不期望的微生物有机体污染。在一些实施方案的微生物群落中，所述第一核酸编码两种或更多种不同的抗微生物肽。在一些实施方案的微生物群落中，所述遗传修饰的微生物有机体还包含编码不靶向所述一种或多种期望的微生物有机体的第二抗微生物肽的第三核酸。所述第三核酸还可以包含所述第二抗微生物肽框内的分泌信号。在一些实施方案的微生物群落中，所述无细胞的第二核酸由质粒或噬菌体组成。在一些实施方案的微生物群落中，所述期望的微生物有机体包含微生物有机体的两种或更多种不同物种，其中所述第一核酸由所述不同物种中的仅一种或多于一种组成。在一些实施方案的微生物群落中，所述微生物群落由微生物组组成。例如，所述微生物组是选自以下的微生物组：胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤，或者任何所列项目中的两种或更多种的组合。在一些实施方案的微生物群落中，所述微生物群落在对象体内原位。一些实施方案的微生物群落是对象自体的，以及是所述对象离体的(例如，用于对象的组织或器官的微生物组的聚居或再聚居。在一些实施方案的微生物群落中，所述微生物群落是工业培养物。

附图的简要说明

图1是显示根据本公开内容的一些实施方案在微生物群落中生产分泌的抗微生物肽的方法的流程图。

图2是显示根据本公开内容的一些实施方案的微生物群落的示意图，所述微生物群落包括通过编码抗微生物肽的核酸和与编码抗微生物肽的核酸具有相同序列的无细胞核酸进行遗传修饰的期望的微生物有机体。

图3是显示根据本公开内容的一些实施方案在微生物群落中生产分泌的抗微生物肽的方法的示意性流程图。

详述

本文提供了用于在微生物群落中生产分泌的抗微生物肽的方法(其在本文中也可称为“生产方法”)。一般而言，生产方法可以包括将编码抗微生物肽的核酸施用至微生物群落中存在的一种或多种微生物有机体，从而遗传修饰所述微生物有机体。遗传修饰的微生物有机体可以将抗微生物肽分泌到微生物群落的环境中，例如以防御不期望的微生物有机体，如污染物和/或病原体。抗微生物肽可以以足以减慢或抑制微生物有机体生长或杀死微生物有机体的水平分泌，所述微生物有机体对抗微生物肽敏感。另一方面，遗传修饰的微生物有机体可以对抗微生物肽具有抗性，并且可以在分泌的抗微生物肽存在下在微生物群落中继续生长和繁殖。许多微生物群落，如微生物组或工业发酵，或在食品或药物或化妆品制造环境中，含有特定比例或化学计量的不同类型的微生物有机体。有利地，本文所述的方法和微生物群落可以通过对微生物有机体进行原位遗传修饰来维持微生物有机体的现有比例或化学计量。此外，微生物群落通常含有适于该微生物群落及其环境的期望的微生物有机体的特定菌株。可以容易地鉴定这些期望的菌株，因此可以被靶向表达编码抗微生物肽的核酸。然而，不期望的微生物有机体可能更难以鉴定，并且它们存在于微生物群落中的时间和位置可能是未知的。因此，通过原位修饰已知的微生物有机体，如本文所述的生产方法和微生物群落可以通过原位提供期望的微生物有机体来有效地和可靠地抑制微生物群落中不期望的微生物有机体的生长和繁殖。同时，可以保留适合微生物群落和环境的期望的微生物有机体的特定菌株，微生物群落内微生物有机体的化学计量也可以保留。

一些实施方案的生产方法允许一种或多种期望的微生物有机体优先于不期望的微生物有机体生长，所述不期望的微生物有机体对微生物群落中的抗微生物肽敏感。微生物群落可以包括期望的微生物有机体，其被遗传修饰以产生由核酸编码的抗微生物肽，利用所述核酸，微生物有机体被遗传修饰(不必向微生物群落中加入外源遗传修饰的微生物有机体)。

根据本公开内容的实施方案，可以通过将编码抗微生物肽的核酸原位施用至微生物群落来遗传修饰微生物群落中期望的微生物有机体(例如，如本文所述遗传修饰的微生物有机体)。例如，可以通过质粒、噬菌体、染色体外阵列、附加体或微型染色体施用核酸。

如本文所用，“微生物群落”具有本领域普通技术人员鉴于本公开内容所理解的其普通和常规的含义。它是指在环境中包含微生物有机体的两种或更多种不同物种的异质群体。例如，微生物群落可以由微生物组或工业或化妆品或药物制造培养物组成。微生物群落可以包含不同分类学分类的微生物有机体，如不同属和/或种、和/或相同物种的不同菌株。例如，微生物群落可以包含至少10⁶、10⁷、10⁸、10⁹、10¹⁰、10¹¹或10¹²种微生物有机体，包括任何两个所列值之间的范围。

本文还提供了微生物群落，其包含期望的微生物有机体，所述微生物有机体经遗传工程化以表达编码抗微生物肽和所述抗微生物肽框内的分泌信号的核酸，其中编码所述抗微生物肽的核酸也存在于所述微生物群落中。参考图2，本公开内容的一些非限制性实施方案包括微生物群落200，其可以含有期望的微生物有机体201，其中的一种或多种可以用核酸220进行遗传修饰210。核酸可以编码抗微生物肽230，其不靶向期望的微生物有机体。核酸还可以编码抗微生物肽框内的分泌信号240，使得肽在表达时包含分泌信号。核酸也可以自由地存在(例如，在微生物群落内的任何细胞或微生物区室210之外)。当通过本公开内容的方法修饰微生物群落时，施用以遗传修饰期望的微生物有机体的核酸中的至少一些可能不会被递送至期望的微生物有机体，而是可能在微生物群落中保持无细胞状态。

在本公开内容中可以使用任何合适的微生物群落。合适的微生物群落包括但不限于微生物组或工业培养物。可以使用任何合适的微生物组。合适的微生物组的实例包括但不限于在胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤中发现的那些。

微生物有机体

如本文所用，“微生物有机体”、“微生物”和这些词根术语的变型(如复数等)具有本领域技术人员鉴于本公开内容所理解的其常规和普通的含义。它们涵盖任何天然存在的物种或者完全合成的原核或真核单细胞有机体，以及古生物物种(Archae species)。因此，该术语可指细菌物种、真菌物种和藻类的细胞。“微生物有机体”和“微生物”在本文中可以互换使用，这些词根术语的相应变型也可以互换使用。

可以根据本文的实施方案使用的合适微生物包括但不限于细菌、酵母和藻类，例如光合微藻。此外，可以合成完全合成的微生物基因组并将其移植到单个微生物细胞中，以产生能够连续自我复制的合成微生物(参见Gibson等人(2010),“Creation of aBacterial Cell Controlled by a Chemically Synthesized Genome,”Science 329:52-56,，在此通过引用以其整体并入)。因此，在一些实施方案中，微生物是完全合成的。遗传元件(包括调节基因表达的元件和编码基因产物的元件(例如细菌素、免疫调节剂、毒物、解毒剂和工业上有用的分子)的期望组合可以在期望底盘(chassis)上组装成部分或完全合成的微生物。用于工业应用的基因工程化的微生物有机体的描述也可见于Wright等人,(2013)“Building-in biosafety for synthetic biology”,Microbiology 159:1221-1235。

根据本文的实施方案，可以使用多种细菌物种和菌株，并且可以提供基于已知物种的“底盘”的遗传修饰的变体或合成细菌。可以根据本文的实施方案使用的具有工业上可应用特性的示例性细菌包括但不限于芽孢杆菌属物种(例如凝结芽孢杆菌(Bacilluscoagulans)、枯草芽孢杆菌(Bacillus subtilis)和地衣芽孢杆菌(Bacilluslicheniformis))，类芽孢杆菌属物种，链霉菌属物种，微球菌属物种，棒杆菌属物种，醋杆菌属物种，蓝细菌属物种，沙门氏菌属物种，红球菌属物种，假单胞菌属物种，乳杆菌属物种，肠球菌属物种，双歧杆菌属物种，拟杆菌属物种，产碱杆菌属物种，克雷伯氏菌属物种，类芽孢杆菌属物种，节杆菌属物种，棒杆菌属物种，短杆菌属物种，水生栖热菌(Thermusaquaticus)，施氏假单胞菌(Pseudomonas stutzeri)，嗜热梭菌(Clostridiumthermocellus)和大肠杆菌(Escherichia coli)。

根据本文的实施方案，可以使用多种酵母物种和菌株，并且可以提供基于已知物种的“底盘”的遗传修饰的变体或合成酵母。可以根据本文的实施方案使用的具有工业上可应用特性的示例性酵母包括但不限于酵母属物种(例如酿酒酵母(Saccharomycescerevisiae)、贝酵母(Saccharomyces bayanus)、布拉氏酵母菌(Saccharomycesboulardii))，假丝酵母属物种(例如产朊假丝酵母(Candida utilis)、克鲁斯假丝酵母(Candida krusei))，裂殖酵母属物种(例如粟酒裂殖酵母(Schizosaccharomyces pombe)、日本裂殖酵母(Schizosaccharomyces japonicas))，毕赤酵母属或汉逊酵母属物种(例如，巴斯德毕赤酵母(Pichia pastoris)或多形汉逊酵母(Hansenula polymorpha))和酒香酵母属物种(例如，克劳森酒香酵母(Brettanomyces claussenii))。

根据本文的实施方案，可以使用多种藻类物种和菌株，并且可以产生基于已知物种的“底盘”的遗传修饰的变体或合成藻类。在一些实施方案中，藻类包含光合微藻类。可用于生物燃料并且可以根据本文的实施方案使用的示例性藻类物种包括布朗葡萄藻(Botryococcus braunii)、小球藻属物种、特氏杜氏藻(Dunaliella tertiolecta)、江蓠属物种、颗石类微藻(Pleurochrysis carterae)和马尾藻属物种。另外，许多藻类物种可用于食物产品、肥料产品、废物中和、环境修复和碳水化合物制造(例如，生物燃料)。

出于任何合适的目的，期望的微生物有机体可有益于微生物群落。在某些实施方案中，期望的微生物有机体对例如微生物群落本身的生长和/或维持、微生物群落所属的较大环境、使用微生物群落的目的等提供益处。在某些实施方案中，本公开内容的微生物群落被不期望的微生物有机体污染。由于任何相关的原因，不期望的微生物有机体可能是不期望的。在一些实施方案中，不期望的微生物是病原体。例如，不期望的微生物有机体可能对微生物群落(例如，包含微生物组的宿主)的宿主有机体(例如，哺乳动物)是致病的。在一些实施方案中，不期望的微生物有机体可能对在微生物群落中或在微生物群落周围生长的有机体是致病的。在一些实施方案中，不期望的微生物有机体是与微生物群落中期望的微生物有机体的生长竞争和/或干扰微生物群落中期望的微生物有机体的生长的微生物有机体。在一些实施方案中，不期望的微生物有机体选自病原体、污染物以及与微生物群落中期望的微生物有机体的生长竞争和/或干扰微生物群落期望的微生物有机体的生长的微生物有机体。

核酸

根据本文所述的一些实施方案的生产方法和微生物群落，抗微生物肽(例如细菌素)由核酸(如DNA、RNA或这些的组合)编码。例如，抗微生物肽(例如细菌素)基因的DNA序列可编码翻译成蛋白质的mRNA转录物，所述蛋白质包含抗微生物肽(如细菌素)，基本上由其组成或由其组成。考虑了核酸可以包含一个或多个非天然存在的核苷酸，例如锁核酸(LNA)、肽核酸(PNA)等。在本文的一些实施方案的方法和组合物中，可以将编码多肽原的多核苷酸递送到微生物中，并且可以稳定地整合到这些微生物的染色体中，或者可以游离于基因组存在，例如在质粒、染色体外阵列、附加体、微型染色体等中。

用于微生物细胞的遗传修饰的示例性载体包括但不限于质粒、染色体外阵列、附加体、微型染色体、病毒(包括噬菌体)和转座元件。另外，应理解，包含期望序列的完整微生物基因组可以在细胞中合成和组装(参见，例如Gibson等人(2010),Science 329:52-56)。因此，在一些实施方案中，合成具有期望特征的微生物基因组(或其部分)，如细菌素多核苷酸，并将其引入到微生物细胞中。

在一些实施方案中，提供了用于将一种或多种期望的细菌素和/或免疫调节剂多核苷酸插入到多核苷酸序列(例如将编码包含细菌素的多肽原的盒插入到表达载体中)的盒。示例性的盒包括但不限于Cre/lox盒或FLP/FRT盒。在一些实施方案中，所述盒定位在质粒上，使得具有编码期望多肽原的期望多核苷酸的质粒可以容易地引入到微生物细胞中。在一些实施方案中，所述盒定位在微生物细胞的基因组中的期望位置。

在一些实施方案中，质粒缀合可用于将期望的质粒从“供体”微生物细胞引入受体微生物细胞中。-Moreno等人,(2013)Multicellular Computing Using Conjugationfor Wiring.PLoS ONE 8(6):e65986，将其在此通过引用以其整体并入。例如，一些实施方案的遗传修饰的期望的微生物有机体可以将编码抗微生物肽的质粒引入到微生物群落中的另一种微生物有机体中。在一些实施方案中，质粒缀合可以通过将缀合质粒从供体微生物细胞引入到受体微生物细胞来遗传修饰受体微生物细胞。不受任何特定理论的限制，包含相同或功能上相同的复制基因组的缀合质粒通常不能共存于相同的微生物细胞中。因此，在一些实施方案中，质粒缀合通过引入新的缀合质粒以取代受体细胞中存在的另一种缀合质粒来“重编程”受体微生物细胞。在一些实施方案中，质粒缀合用于工程化(或重新工程化)具有编码多肽原的特定核酸或编码不同多肽原的不同核酸的组合的微生物细胞。根据一些实施方案，提供了包含不同核酸的多种缀合质粒，所述核酸包含多种不同的多肽原。质粒可以包含本文所述的额外遗传元件，例如启动子、翻译起始位点等。在一些实施方案中，在供体细胞的集合中提供了多种缀合质粒，从而可以选择包含期望质粒的供体细胞用于质粒缀合。在一些实施方案中，选择细菌素的特定组合和/或比例，并将适合的供体细胞(编码特定的多肽原)与目的微生物细胞缀合，以将包含该组合的缀合质粒引入到受体细胞中。

分泌信号

为了促进抗微生物肽的分泌，抗微生物肽还可以包含合适的分泌信号。已经显示跨分类群的分泌系统共有核心特征，包括完整的膜易位设备，其通常是异源三聚体蛋白复合物，如细菌中的SecYEG复合物或真核生物中的Sec61复合物或这些复合物成员的古菌同源物，如古菌中的SecY/Sec61α和SecE/Sec61γ同源物(参见，例如，等人,Cell 91:563-566(1997))，将其通过引用以其整体并入本文。

已经描述了可应用于革兰氏阳性、革兰氏阴性或两种类型细菌的几种典型的细菌分泌系统，并在例如Green等人,Microbiol Spectr.4:doi:10.1128/microbiolspec.VMBF-0012-2015(2016)中进行了综述，将其在此通过引用以其整体并入本文。以下重现的Green等人的表1突出了细菌Sec、Tat、T1SS、T2SS、T3SS、T4SS、T5SS、T6SS、SecA2、分拣酶、注射体和T7SS分泌系统的特征。对于真核微生物有机体，也可以使用合适的分泌系统。例如，已经详细地表征了酿酒酵母中的分泌途径(参见，例如，Novick等人,Cell 25:461-469(1981)，将其通过引用以其整体并入本文)。

表1：细菌分泌系统的类别

易位到细胞外环境可以由信号序列启动。信号序列在生命领域中是典型保守的，并且例如，N-末端信号序列可以包含带正电的N末端、疏水性氨基酸残基的核心和极性更大的C末端(等人,Cell 91:563-566(1997))。可选地，对于C-末端信号序列，这些特征可以是相反的顺序。各种信息学工具可用于鉴定预测的原核和真核信号序列，例如，如Armenteros等人,Nature Biotechnology,37,420-423(2019)(将其通过引用以其整体并入)中所述的SignalP 5.0。在万维网上以www.cbs.dtu.dk/services/SignalP获取SignalP的当前版本。

应理解，根据本文的实施方案，期望的微生物有机体是已知的，因此，可以选择合适的分泌信号(与相关的分泌系统相容)。

抗微生物肽

如本文所用，“抗微生物肽”(包括这些词根术语的变型，如“抗微生物肽”)具有本领域普通技术人员鉴于本公开内容所理解的其常规和普通的含义。它是指一类杀死微生物有机体或阻止微生物有机体生长的肽。尽管抗微生物肽通常被称为一类靶向微生物有机体的无脊椎动物和脊椎动物基因产物，但细菌素通常被称为一类靶向微生物有机体的微生物基因产物。然而，为了简洁起见，本文所用的“抗微生物肽”广泛地涵盖经典的抗微生肽(例如，其赋予对抗微生物有机体的先天免疫活性)以及细菌素。因此，合适的抗微生物肽包括源自任何来源(例如，来源于原核生物或真核生物，如哺乳动物、真菌、植物等，或者部分或完全合成的)的多肽，其减少或抑制微生物有机体的生长或者杀死微生物有机体。在一些实施方案中，抗微生物肽包含以下，基本上由以下组成或由以下组成：无脊椎动物和脊椎动物的先天免疫系统的肽。因此，在一些实施方案中，抗微生物肽包括一类靶向微生物有机体的无脊椎动物和脊椎动物基因产物。

合适的抗微生物肽的实例可以见于例如可在万维网上以aps.unmc.edu/AP/访问的抗微生物肽数据库(The Antimicrobial peptide Database)，将其通过引用以其整体并入本文。已经鉴定并编目了超过1000种抗微生物肽及其变体。抗微生物肽数据库描述在Wang等人(2016),Nucleic Acids Res.44(Database issue):D1087-D1093中，将其通过引用以其整体并入本文。适用于本文的实施方案(如在生产方法和/或微生物群落中)的抗微生物肽的实例包括细菌素、抗细菌肽、抗病毒肽、抗HIV肽、抗真菌肽、抗寄生虫肽和抗癌肽，如dermaseptins(例如，Dermaseptin-B2)、abaecin、Ct-AMP1、andropin、apidaecin、天蚕素(cecropin)、角质毒素(ceratotoxin)、dermacidin、Maximin H5、家蚕抗菌肽(moricin)、蜂毒肽(melittin)、爪蟾抗菌肽(magainin)、铃蟾抗菌肽(bombinin)、brevinin、esculentin、蟾蜍抗菌肽(buforin)、CAP18、LL37、protegrin、prophenin、indolicidin、鲎素肽(tachyplesins)、防御素(defensin)、果蝇霉素(drosomycin)、aurein 1.1、乳铁蛋白肽B(Lactoferricin B)和海利霉素(Heliomicin)或者任何所列项目中的两种或更多种的组合。在一些实施方案中，抗微生物肽包含细菌素，dermaseptins(例如，Dermaseptin-B2)、abaecin、Ct-AMP1、andropin、apidaecin、天蚕素、角质毒素、dermacidin、Maximin H5、家蚕抗菌肽、蜂毒肽、爪蟾抗菌肽、铃蟾抗菌肽、brevinin、esculentin、蟾蜍抗菌肽、CAP18、LL37、protegrin、prophenin、indolicidin、鲎素肽、防御素、果蝇霉素、aurein1.1、乳铁蛋白肽B和海利霉素或者任何所列项目中的两种或更多种的组合。在一些实施方案中，本公开内容的抗微生物肽包括天然存在的抗微生物肽或者其突变体或变体，或者编码它们的核酸。在一些实施方案中，本公开内容的抗微生物肽包括非天然存在的抗微生物肽(如部分或完全合成的抗微生物肽或变体抗微生物肽)或编码其的核酸。在一些实施方案中，本公开内容的抗微生物肽是非天然存在的肽序列或编码其的核酸。在一些实施方案中，抗微生物肽与参考抗微生物肽(例如Dermaseptin-B2、Abaecin、Ct-AMP1、Andropin、Aurein 1.1、乳铁蛋白肽B或海利霉素，或SEQ ID NO:4-450(偶数)和699-737(奇数)中的任一个)具有至少约70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％的同一性，包括任何两个所列值之间的范围，例如70％-99％、75％-99％、80％-99％、85％-99％、90％-99％、95％-99％、97％-99％、70％-95％、75％-95％、80％-95％、85％-95％、90％-95％、70％-90％、75％-90％、80％-90％和85％-90％。这种抗微生物肽可以被称为“变体”抗微生物肽。可以使用BLAST软件(默认参数)来确定同一性百分比(Altschul,S.F.等人,(1990)“Basic local alignment search tool.”J.Mol.Biol.215:403-410，在万维网上以blast.ncbi.nlm.nih.gov访问)。

在一些实施方案中，本公开内容的抗微生物肽包含细菌素,基本上由其组成或由其组成。如本文所用，“细菌素”和该词根术语的变型具有本领域技术人员鉴于本公开内容所理解的其常规和普通的含义。它是指这样的多肽，所述多肽由宿主细胞分泌并能够中和不同于制备多肽的单独宿主细胞的至少一种细胞，包括与宿主细胞克隆相关的细胞和其它微生物细胞。表达特定“免疫调节剂”(本文更详细论述的)的细胞对特定细菌素或细菌素组的中和作用是免疫的。因此，细菌素可以中和产生细菌素的细胞和/或其它微生物细胞，只要这些细胞不产生适当的免疫调节剂。因此，宿主细胞可以通过分泌细菌素对多种其它微生物有机体发挥细胞毒性或生长抑制作用。示例性的细菌素示于SEQ ID NO:4-450(偶数)和699-737(奇数)中。编码这些细菌素的示例性核酸提供为SEQ ID NO:5-451(奇数)和700-738(偶数)。细菌素的详细描述，包括使用细菌素以控制微生物细胞生长的方法和组合物，可以见于例如美国专利号9,333,227，将其在此通过引用以其全文并入。“细菌素”不受多肽来源的限制，并且例如考虑了涵盖任何细菌素，如天然存在的细菌素、合成的细菌素及其变体和组合。本文详细描述了合适的细菌素的实例。

尽管许多细菌素是天然存在的(例如，SEQ ID NO:4-450(偶数)和699-737(奇数)中所示的天然存在的细菌素)，但本领域技术人员将理解，在本文所述的方法、系统和试剂盒的一些实施方案中，细菌素包含除SEQ ID NO:4-450(偶数)和699-737(奇数)的细菌素和编码SEQ ID NO:4-450(偶数)和699-737(奇数)的核苷酸序列之外的天然存在的细菌素、或者非天然存在的细菌素或者合成细菌素(如工程化的细菌素)或者其变体(其也可以是一些实施方案的一种工程化的细菌素)。在一些实施方案中，相对于野生型细菌素，工程化的细菌素在相同或不同微生物或微生物物种上具有增强或降低水平的细胞毒性或生长抑制活性。在一些实施方案中，抗微生物肽(或细菌素)不包含羊毛硫细菌素。

几种基序已被认为是细菌素的特征。例如，基序YGXGV(SEQ ID NO:2)是IIa类细菌素的N-末端共有序列特征，其中X是任意氨基酸残基。因此，在一些实施方案中，候选(或变体)细菌素(例如工程化的细菌素)包含与SEQ ID NO:2具有至少约50％同一性的N-末端序列或其变体。在一些实施方案中，候选(或变体)细菌素(例如工程化的细菌素)包含含有SEQID NO:2的N-末端序列。另外，一些IIb类细菌素包含GxxxG基序。不受任何特定理论的限制，认为GxxxG基序可以介导细胞膜中螺旋蛋白之间的缔合，例如以通过细胞膜相互作用促进细菌素介导的中和。因此，在一些实施方案中，细菌素(例如工程化的细菌素)包含促进与细胞膜相互作用的基序。在一些实施方案中，细菌素包含GxxxG基序。任选地，包含GxxxG基序的细菌素可以包含螺旋结构。除了本文所述的结构之外，本文所用的“细菌素”还涵盖对微生物细胞具有与本文明确提供的任何细菌素基本相同作用的结构。

已经鉴定和表征了许多细菌素。不受理论的限制，可以将示例性的细菌素分类为通常经历翻译后修饰的“I类”细菌素和通常未修饰的“II类”细菌素。关于分类细菌素的额外信息可以见于Cotter,P.D.等人，“Bacteriocins-a viable alternative toantibiotics”Nature Reviews Microbiology(2013)11:95-105，将其通过引用以其整体并入本文。

根据本文中的实施方案，可以使用许多细菌素。示例性的细菌素示于SEQ ID NO:4-450(偶数)和699-737(奇数)。编码这些细菌素的示例性核酸提供为SEQ ID NO:5-451(奇数)和700-738(偶数)。细菌素和一些编码细菌素的多核苷酸序列的详细描述(包括使用细菌素控制微生物细胞生长的方法和组合物)可以见于例如美国专利号9,333,227，将其通过引用以其整体并入本文。在美国专利号9,333,227的表1.2中教导了合适的细菌素的一些实例，将其通过引用以其整体并入本文。“细菌素”不受多肽来源的限制，并且例如预期了涵盖任何细菌素，如天然存在的细菌素、合成的细菌素及其变体和组合。本文详细描述了合适的细菌素的实例。

一些抗微生物肽(如细菌素)具有细胞毒性活性(例如“杀菌剂”作用)，因此可以杀死微生物有机体，例如细菌、酵母、藻类、合成微生物等。一些抗微生物肽(如细菌素)可以例如通过阻止细胞周期而抑制微生物有机体(例如细菌、酵母、藻类、合成微生物等)的繁殖(例如“抑菌”作用)。不受理论的限制，细菌素可以以多种方式实现靶标微生物细胞的中和。例如，细菌素可渗透细胞壁，从而使细胞壁去极化并干扰呼吸。

“抗生素”和该词根术语的变型具有本领域技术人员鉴于本公开内容所理解的其常规和普通的含义。它是指可以杀死至少一种微生物细胞或阻止至少一种微生物细胞生长的代谢途径的代谢物或中间体。一些抗生素可以由微生物细胞(例如细菌)产生。一些抗生素可以化学合成。应理解，细菌素不同于抗生素，至少在于细菌素是指基因产物(在一些实施方案中，其经历额外的翻译后加工)或其合成类似物，而抗生素是指代谢途径的中间体或产物或其合成类似物。

在一些实施方案中，抗微生物肽(如细菌素)包含经历翻译后修饰(例如切割)或者添加一个或多个官能团的多肽。

在一些实施方案中，包含两种或更多种抗微生物肽(如细菌素)或其部分的融合多肽比所述两种或更多种抗微生物肽或其部分的任一种单独的抗微生物肽具有针对更广范围的微生物有机体的中和活性。例如，已经显示杂合抗微生物肽显示出针对致病革兰氏阳性和革兰氏阴性细菌的抗微生物活性(等人,(2012),FEBS Open Bio,2:12-19)。应注意，Ent35-MccV融合细菌素从N-末端到C-末端包含N-末端甘氨酸、肠道菌素CRL35、包含三个甘氨酸的接头和C末端小菌素V。

本文考虑了抗微生物肽(如细菌素)可包含两种或更多种多肽的融合物，例如具有抗微生物(如细菌素)活性的两种或更多种多肽。在一些实施方案中，抗微生物肽或候选抗微生物肽包含嵌合蛋白。在一些实施方案中，变体抗微生物肽(如细菌素)或工程化的抗微生物肽(如工程化的细菌素)包含融合多肽，所述融合多肽包含两种或更多种抗微生物肽(如细菌素)。在一些实施方案中，变体抗微生物肽(如细菌素)或工程化的抗微生物肽(如细菌素)包含嵌合蛋白，所述嵌合蛋白包含两种或更多种抗微生物肽(如细菌素)或其片段。在一些实施方案中，融合物的两种或更多种抗微生物肽包含SEQ ID NO:4-450(偶数)和699-737(奇数)的多肽，和/或由SEQ ID NO:5-451(奇数)和700-738(偶数)的核酸编码的多肽，或其变体或修饰。在一些实施方案中，融合多肽具有比单独的抗微生物肽更广谱的活性，例如具有针对更多微生物有机体的中和活性、在更宽范围的环境条件下的中和活性和/或更高效率的中和活性。在一些实施方案中，融合多肽包含两种、三种、四种、五种、六种、七种、八种、九种或十种抗微生物肽。在一些实施方案中，两种或更多种抗微生物肽多肽经由共价键(例如肽键)彼此融合。在一些实施方案中，接头定位在融合多肽的两个单独的抗微生物多肽之间。在一些实施方案中，接头包含一个或多个甘氨酸，例如约1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19或20个甘氨酸。在一些实施方案中，在细胞内切割接头以产生包含在融合蛋白中的单独的抗微生物肽(如细菌素)。在一些实施方案中，本文提供的变体抗微生物肽(如变体细菌素)或工程化的抗微生物肽(如工程化的细菌素)包含修饰以提供相对于未修饰的或候选的抗微生物肽(例如细菌素)的期望活性谱。例如，变体抗微生物肽(例如细菌素)或工程化的抗微生物肽(例如细菌素)可以针对与未修饰的或候选的抗微生物肽(例如细菌素)相同的有机体具有增强的或降低的活性。可选地，修饰的抗微生物肽(例如细菌素)可以针对有机体具有增强的活性，针对所述有机体，未修饰的或候选的抗微生物肽(例如细菌素)具有较低的活性或无活性。

在一些实施方案中，基于期望的抗微生物调节的类型和所靶向的微生物有机体的特定分类类别、物种或菌株，选择抗微生物肽的特定中和活性或活性范围(例如，细胞毒性或阻止微生物繁殖)。因此，在一些实施方案中，选择特定的抗微生物肽或抗微生物肽的组合。例如，在一些实施方案中，基于被调节的不期望的微生物有机体，将期望的微生物有机体工程化以表达特定的抗微生物肽。在一些实施方案中，例如，如果要杀死污染的不期望的微生物有机体，则提供至少一种细胞毒性抗微生物肽(如细胞毒性细菌素)。在一些实施方案中，选择有效对抗通常在特定培养物、微生物组、特定地理位置或者生长在特定地理位置或工业培养物中的特定类型的培养物中出现的污染物的细菌素或细菌素的组合。在一些实施方案中，例如需要调节微生物细胞比例的实施方案，提供了抑制微生物繁殖的抗微生物肽。不受理论的限制，许多细菌素可对微生物有机体具有中和活性，所述微生物有机体通常占据与产生细菌素的物种相同的生态位。因此，在一些实施方案中，当需要抗微生物肽活性的特定谱时，细菌素选自占据与细菌素所靶向的一种或多种微生物有机体相同(或相似)生态位的宿主物种。在一些实施方案中，选择抗微生物肽的特定组合和/或比例以靶向单一微生物有机体(其可以包括靶向一种或多于一种该类型的微生物有机体，例如克隆相关的微生物有机体)。例如，与单一抗微生物肽相比，可通过抗微生物肽的预定混合物和/或比例更有效地靶向特定类型的微生物有机体。

例如，在一些实施方案中，抗微生物肽需要抗真菌活性(如抗酵母活性)。已经鉴定了许多具有抗真菌活性的细菌素。例如，来自芽孢杆菌的细菌素已经显示出对酵母菌株具有中和活性(参见Adetunji和Olaoye(2013)Malaysian Journal of Microbiology 9:130-13，将其在此通过引用以其整体并入)，粪肠球菌(Enterococcus faecalis)肽(WLPPAGLLGRCGRWFRPWLLWLQ SGAQY KWLGNLFGLGPK,SEQ ID NO:1)已经显示出对念珠菌属物种具有中和活性(参见Shekh和Roy(2012)BMC Microbiology 12:132，将其在此通过引用以其整体并入)，以及来自假单胞菌的细菌素已经显示出对真菌如新月弯孢菌(Curvularialunata)、镰刀菌属物种、长蠕孢属物种和蠕孢菌属(Biopolaris)物种具有中和活性(Shalani和Srivastava(2008)The Internet Journal of Microbiology.Volume 5Number2.DOI:10.5580/27dd——在万维网上以archive.ispub.com/journal/the-internet-journal-of-microbiology/volume-5-number-2/screening-for-antifungal-activity-of-pseudomonas-fluorescens-against-phytopathogenic-fungi.html#sthash.d0Ys03UO.1DKuT1US.dpuf访问，将其在此通过引用以其整体并入)。例如，来自枯草芽孢杆菌的杀葡孢菌素(botrycidin)AJ1316(参见Zuber,P等人,(1993)PeptideAntibiotics.In Bacillus subtilis and Other Gram-Positive Bacteria:Biochemistry,Physiology,and Molecular Genetics ed.Sonenshein等人,pp.897-916,American Society for Microbiology，将其在此通过引用以其整体并入)和alirin B1(参见Shenin等人,(1995)Antibiot Khimioter 50:3-7，将其在此通过引用以其整体并入)已经显示出具有抗真菌活性。因此，在一些实施方案(例如其中需要中和真菌微生物有机体的实施方案)中，细菌素包含杀葡孢菌素AJ1316或alirin B1中的至少一种。

例如，在一些实施方案中，在特定环境(例如特定微生物群落的培养物)中的抗微生物肽活性是期望的，并且以预定的混合物和/或比例选择抗微生物肽以便杀死不同于期望的微生物有机体的不期望的微生物有机体或阻止不同于期望的微生物有机体的不期望的微生物有机体的生长。可以选择通常由期望的微生物产生的细菌素，因为期望的微生物有机体可以已经产生针对这些细菌素的相关免疫调节剂，或者可以容易地被工程化以产生免疫调节剂。因此，所选择的细菌素可靶向不期望的微生物细胞(包括尚未存在于微生物群中的不期望的微生物细胞)，同时几乎不引起或不引起期望的微生物有机体的中和。例如，在一些实施方案中，选择特定比例的抗微生物肽以便中和通常在蓝细菌培养环境中发现的入侵微生物有机体，同时保留蓝细菌。已经在多种蓝细菌物种中鉴定了保守性细菌素多肽的簇。例如，在至少43种蓝细菌物种中已经鉴定出至少145个推定的细菌素基因簇，如Wang等人,(2011),Genome Mining Demonstrates the Widespread Occurrence of GeneClusters Encoding Bacteriocins in Cyanobacteria.PLoS ONE 6(7):e22384中所报告的，将其在此通过引用以其整体并入。示例性的蓝细菌细菌素显示在SEQ ID NO.:420、422、424、426、428、430、432、434、436、438、440、442、444、446、448和450中。

在一些实施方案中，选择一种或多种抗微生物肽活性，并提供包含期望化学计量的抗微生物肽的多肽原。一些实施方案的抗微生物肽可以最初在多肽原中产生，所述多肽原包含一个或多个抗微生物肽序列，并且其可以被切割以产生单独的抗微生物肽。多肽原可以包含拷贝数目的单独抗微生物肽，使得在切割时抗微生物肽以特定的化学计量存在。因此，两种或更多种不同抗微生物肽的混合物可以以期望的比例或化学计量产生。制备期望的比例和期望的化学计量的抗微生物肽的方法描述在例如PCT公开号WO2019/046577中，将其通过引用以其整体并入本文。在一些实施方案中，多肽原由微生物细胞的翻译机制(例如核糖体等)产生。多肽原可以经历切割(例如通过切割酶如天然存在的或合成的蛋白酶进行加工)以产生抗微生物肽本身的多肽。因此，在一些实施方案中，抗微生物肽由前体多肽产生。编码多肽原的多核苷酸可以例如使用核酸合成和/或分子克隆来制备，并且可以用于产生多肽原。

在一些实施方案中，抗微生物肽(及其比例)可以基于其中和一种或多种入侵有机体的能力来选择，所述入侵有机体可能试图在特定的培养物中生长。在一些实施方案中，抗微生物肽(及其比例)可以基于其限制特定有用微生物菌株在环境中，例如在工业原料中，或在发酵罐中，或在食品、药物或化妆品制造环境中，或在组织环境如肠或皮肤微生物组中生长的能力，或在维持或调节植物、植物根和/或土壤中的微生物群，或保持或维持物、药物或化妆品产品的质量的能力来选择。在一些实施方案中，基于现有环境中的一种或多种微生物菌株或微生物菌株群来选择一种或多种抗微生物肽活性(和/或比例)。例如，在一些实施方案中，如果在环境中鉴定出特定类别的入侵者或可能的入侵者，并且可以选择中和抗微生物肽的混合物(及其比例)来中和所鉴定的入侵者。在一些实施方案中，选择抗微生物肽以中和环境中所有或基本上所有的微生物细胞，例如以消除培养环境中的工业培养物，使得可以将新的工业培养物引入培养环境中，或者防止或抑制药物或化妆品制造环境的污染，或者防止或最小化食品、药物或化妆品的污染或腐败。

免疫调节剂

在一些实施方案中，特定的免疫调节剂或免疫调节剂的特定组合赋予对特定抗微生物肽(例如细菌素等)、特定种类或类别的抗微生物肽或抗微生物肽的特定组合的免疫。示例性抗微生物肽(例如细菌素等)鉴定在美国专利第9,333,227号的表2中，免疫调节剂可赋予对所述示例性抗微生物肽的免疫。示例性的免疫调节剂序列包括，例如SEQ ID NO:452-540(偶数)和SEQ ID NO:453-541(奇数)中的任一个。

虽然美国专利第9,333,227号的表2和本申请的序列表鉴定了示例性免疫调节剂的“来源有机体”，但根据本文的一些实施方案，这些免疫调节剂可以容易地在其它天然存在的、遗传修饰的或合成的微生物中表达，以提供期望的抗微生物肽免疫活性。因此，如本文所用，“免疫调节剂”具有本领域技术人员鉴于本公开内容所理解的其常规和普通的含义，并且不仅是指本文明确提供的结构，而且是指与本文所述的“免疫调节剂”结构具有基本上相同作用的结构，包括完全合成的免疫调节剂和提供对在功能上等同于本文公开的抗微生物肽的抗微生物肽(例如细菌素等)的免疫的免疫调节剂。

编码SEQ ID NO:452-540(偶数)的多肽的示例性多核苷酸序列显示在SEQ ID NO:453-541(奇数)中。本领域技术人员将容易理解遗传密码是简并的，而且，密码子使用可以根据表达基因产物的特定有机体而变化，因此，特定的多肽可以由一种以上的多核苷酸编码。在一些实施方案中，基于表达抗微生物肽免疫调节剂的有机体的密码子使用来选择编码抗微生物肽免疫调节剂的多核苷酸。在一些实施方案中，基于表达抗微生物肽免疫调节剂的特定有机体，对编码抗微生物肽免疫调节剂的多核苷酸进行密码子优化。

大范围的功能性免疫调节剂可以包含本文公开的免疫调节剂的特征，从而提供对SEQ ID NO:452-540(偶数)的免疫调节剂的大程度的同一性。在一些实施方案中，免疫调节剂与美国专利第9,333,227号的表2中的任一种多肽具有至少约50％的同一性，例如至少约50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性或者由前述值中的任两个定义的同一性范围。

启动子

启动子是本领域熟知的。启动子可用于驱动一个或多个基因的转录。在一些实施方案中，启动子驱动如本文所述的编码抗微生物肽或蛋白酶的多核苷酸的表达。在一些实施方案中，启动子驱动编码如本文所述的包含两种或更多种抗微生物肽的多肽原的多核苷酸的表达。在一些实施方案中，启动子驱动免疫调节剂多核苷酸的表达。在一些实施方案中，启动子驱动如本文所述的编码抗微生物肽的多核苷酸的表达，但微生物细胞不表达针对这些抗微生物肽中的一种或多种的免疫调节剂(例如，细胞可缺乏驱动免疫调节剂转录的启动子，或可缺乏编码免疫调节剂的核酸)。在一些实施方案中，启动子在微生物细胞中驱动编码包含两种或更多种抗微生物肽的多肽原的多核苷酸的表达，但微生物细胞不表达针对这些抗微生物肽中的一种或多种的免疫调节剂(例如，细胞可缺乏驱动免疫调节剂转录的启动子，或可缺乏编码免疫调节剂的核酸)。一些启动子可以始终驱动转录(“组成型启动子”)。一些启动子可以仅在选择的环境下驱动转录(“条件性启动子”)，例如取决于环境条件、化合物、基因产物、细胞周期的阶段等的存在与否。

本领域技术人员将理解，根据期望的表达活性，可以选择适当的启动子，并将其与待表达的核酸序列顺式放置。在本文的SEQ ID NO:544-698中提供了具有示例性活性并且在本文的一些实施方案中有用的示例性启动子。本领域技术人员将理解，一些启动子与特定的转录机制(例如RNA聚合酶、一般转录因子等)相容。因此，尽管对于本文所述的一些启动子鉴定了相容的“物种”，但考虑了在一些实施方案中，这些启动子可以容易地在除了所鉴定的物种之外的微生物中发挥功能，例如在具有相容的内源性转录机制的物种，包含相容的转录机制的遗传修饰的物种，或包含相容的转录机制的完全合成的微生物有机体中。

本文中SEQ ID NO:544-698的启动子可以从Biobricks基金会公开获得。注意，Biobricks基金会鼓励根据BioBrick^TM公共协议(BPA)使用这些启动子。SEQ ID NO:544-698的启动子仅以非限制性实例的方式提供。本领域技术人员将容易地认识到，根据本文的一些实施方案，可以容易地使用上述启动子的许多变体和许多其它启动子(包括从天然存在的有机体分离的启动子、其变体和完全合成的启动子)。

应理解，本文所述的任何“编码”多核苷酸(例如抗微生物肽多核苷酸、免疫多核苷酸或者编码包含两种或更多种抗微生物肽的多肽原的核苷酸)通常在期望启动子的控制下表达。在一些实施方案中，单一“编码”多核苷酸在单一启动子的控制下。在一些实施方案中，两个或更多个“编码”多核苷酸在单一启动子的控制下，例如两个、三个、四个、五个、六个、七个、八个、九个或十个多核苷酸。

通常，特定转录物的翻译起始由转录物的编码序列的5’端或5’处的特定序列调节。例如，编码序列可以从被配置为与起始tRNA配对的起始密码子开始。尽管天然存在的翻译系统通常使用Met(AUG)作为起始密码子，但容易理解，可以将起始tRNA工程化以结合任何期望的三联体，因此，在某些实施方案中，除AUG以外的三联体也可作为起始密码子发挥功能。另外，起始密码子附近的序列可促进核糖体组装，例如Kozak序列((gcc)gccRccAUGG，SEQ ID NO:542，其中R代表“A”或“G”)或典型真核翻译系统中的内部核糖体进入位点(IRES)或典型原核翻译系统中的Shine-Delgarno序列(GGAGGU，SEQ ID NO:543)。因此，在一些实施方案中，包含“编码”多核苷酸序列的转录物，例如抗微生物肽多核苷酸或免疫多核苷酸，或编码包含两种或更多种抗微生物肽的多肽原的核苷酸，包含适当的起始密码子和翻译起始序列。在一些实施方案中，例如，如果两个或更多个“编码”多核苷酸序列以顺式定位在转录物上，则每个多核苷酸序列包含适当的起始密码子和翻译起始序列。在一些实施方案中，例如，如果两个或更多个“编码”多核苷酸序列以顺式定位在转录物上，则这两个序列在单一翻译起始序列的控制之下，并且任一个提供单一多肽，所述多肽能够与顺式编码的两个多肽一起发挥功能。

在微生物群落中原位生产分泌的抗微生物肽的方法

在一些实施方案中，描述了在微生物群落中原位生产分泌的抗微生物肽的方法(为了简明起见，在本文中也称为“生产方法”)。所述方法可以包括向微生物群落的至少一种期望的微生物有机体原位施用核酸，其中核酸编码抗微生物肽，所述抗微生物肽不杀死期望的微生物有机体或不阻止期望的微生物有机体的繁殖。因此，可以将期望的微生物有机体配置成表达抗微生物肽。所述方法可以包括允许遗传修饰的期望的微生物有机体在群落中生长，使得其分泌抗微生物肽。

参考图1，显示了根据一些非限制性实施方案在微生物群落中原位生产分泌的抗微生物肽的方法(“生产方法”)100的流程图。生产方法可以包括将期望的微生物有机体鉴定为微生物群落的成员110(例如微生物组或工业培养物中的微生物群落)。生产方法可以包括将核酸原位施用至微生物群落的至少一种期望的微生物有机体，其中核酸编码不杀死所鉴定的期望的微生物有机体或不阻止所鉴定的期望的微生物有机体的繁殖的抗微生物肽。因此，至少一种期望的微生物有机体被遗传修饰以表达抗微生物肽120。例如，经遗传修饰的期望的微生物有机体可以包含在本文所述启动子控制下的编码抗微生物肽的核酸。转到框130，生产方法可以包括允许遗传修饰的期望的微生物有机体在微生物群落中生长，其中遗传修饰的期望的微生物有机体分泌抗微生物肽。抗微生物肽可以杀死微生物群落中的任何微生物有机体或阻止微生物群落中的任何微生物有机体的生长，所述微生物有机体对所述抗微生物肽敏感。期望的微生物有机体，包括遗传修饰的期望的微生物有机体，可以抵抗抗微生物肽的抗微生物作用。例如，如果抗微生物肽包含细菌素，则遗传修饰的期望的微生物有机体可以产生细菌素的免疫调节剂。例如，微生物群落可以在对象的微生物组中，并且核酸可以在体内递送。例如，微生物群落可以在对象的微生物组的样品中，并且核酸可以离体递送，并且可以将包含遗传修饰的期望的微生物有机体的微生物组施用至对象以定殖(或再定殖)对象的组织或器官，例如以代替失调的微生物组。考虑了本文所述的生产方法可以将微生物群落(如微生物组)中的任何微生物有机体转化为具有抵抗不期望的微生物有机体的抗微生物特性的益生菌。

可以使用多种合适的技术来施用编码如本文所述的抗微生物肽的核酸。编码抗微生物肽的核酸也可以称为“抗微生物肽核酸”。在一些实施方案的生产方法和组合物中，微生物被遗传修饰以包含编码并能够表达一种或多种如本文所述的抗微生物肽的核酸序列。

在本文的一些实施方案的生产方法和组合物中，可以将编码一种或多种抗微生物肽的多核苷酸递送至微生物，并且所述多核苷酸可以被稳定地整合到这些微生物的染色体中，或者可以游离于基因组存在，例如在质粒、染色体外阵列、附加体、微型染色体等中。用于分子克隆和将核酸引入微生物有机体中的技术描述在例如Green和Sambrook,“Molecular Cloning:A Laboratory Manual,”第4版,Cold Spring Harbor LaboratoryPress,2012中。

在一些实施方案中，生产方法包括将期望的微生物有机体鉴定为微生物群落的成员。例如，可以对微生物群落或其培养物的样品进行核酸测序，如16S测序，以鉴定微生物群落中的微生物有机体。例如，可以基于微生物群落的期望特性，例如目的化合物的发酵或代谢来选择期望的微生物有机体。生产方法还可以包括将核酸原位施用至微生物群落的至少一种期望的微生物有机体。核酸可以编码不杀死所鉴定的期望的微生物有机体或不阻止所鉴定的期望的微生物有机体的繁殖的抗微生物肽。抗微生物肽可以如本文所述。因此，对期望的微生物有机体(或多种有机体)进行遗传修饰以表达抗微生物肽。例如，编码抗微生物有机体的核酸可以与期望的微生物有机体中的启动子可操作地连接。所述方法还可以包括允许遗传修饰的期望的微生物有机体在微生物群落中生长，使得遗传修饰的期望的微生物有机体分泌抗微生物肽。在一些实施方案的生产方法中，如果存在对抗微生物肽缺乏免疫力的不期望的微生物有机体，则抗微生物肽可杀死不期望的微生物有机体或阻止不期望的微生物有机体的生长。

注意，，在没有向微生物群落中加入任何额外的微生物有机体的情况下，通过将编码抗微生物肽的核酸原位加入到微生物群落中期望的微生物有机体中，微生物群落可以包含遗传修饰的微生物有机体。另外，适于微生物群落和/或群落所处环境的菌株的期望的微生物有机体可保留在微生物群落中。因此，在一些实施方案的生产方法中，从施用所述核酸到允许遗传修饰的期望的微生物有机体生长，所述期望的微生物有机体彼此之间的比例基本上保持不变。在一些实施方案中，从施用所述核酸到允许遗传修饰的期望的微生物有机体生长，期望的微生物有机体彼此之间的比例改变小于30％、小于25％、小于20％、小于15％、小于10％、小于5％、小于3％、小于2％、小于1％或更小，或由前述值中的任两个限定的范围内的百分比(例如，1-5％、1-30％、1-15％、5-30％、5-15％、10-30％、10-15％、15-30％)。在一些实施方案中，从施用核酸之前和之后(例如，在允许遗传修饰的期望的微生物有机体生长之前)，期望的微生物有机体彼此之间的比例基本上保持不变。在一些实施方案中，从施用核酸之前和之后(例如，在允许遗传修饰的期望的微生物有机体生长之前)，期望的微生物有机体彼此之间的比例改变小于30％、小于25％、小于20％、小于15％、小于10％、小于5％、小于3％、小于2％、小于1％或更小，或由前述值中的任两个限定的范围内的百分比(例如，1-5％、1-30％、1-15％、5-30％、5-15％、10-30％、10-15％、15-30％)。在一些实施方案中，所述方法不包括向微生物群落中加入任何微生物有机体。

在不受理论限制的情况下，考虑了产生靶向不期望的微生物有机体的抗微生物肽足以赋予微生物群落益处。因此，在一些实施方案的生产方法或微生物群落中，在不施用编码相应免疫调节剂的核酸的情况下施用编码抗微生物肽的核酸。例如，期望的微生物有机体可以已经包含抗微生物肽的免疫调节剂(如果抗微生物肽包含细菌素)，或者期望的微生物可以是对抗微生物肽不敏感的分类群或菌株。可以凭经验(例如通过直接培养)或通过鉴定对抗微生物肽的非敏感性是已知特征的微生物有机体的分类学分类、物种或菌株来确定期望的微生物有机体的非敏感性。在一些实施方案的生产方法的中，没有将编码抗微生物肽的免疫调节剂的核酸施用至所述至少一种期望的微生物有机体。

还考虑了尽管期望的微生物有机体可能是已知的或容易鉴定的，但不期望的微生物有机体可具有未知的和/或未鉴定的特征。例如，不期望的微生物有机体的身份可能是未知的，或者微生物群落中存在不期望的微生物有机体的时间和/或位置可能是未知的。因此，在一些实施方案的生产方法或微生物群落中，在所述分泌之前，微生物群落被一种或多种未知身份的不期望的微生物有机体污染。在一些实施方案中，不期望的微生物有机体的身份是已知的。在一些实施方案中，不期望的微生物有机体是微生物群落的常见污染物或病原体。在一些实施方案中，不期望的微生物有机体的抗微生物肽敏感性是已知的。在一些实施方案中，不期望的微生物有机体的抗微生物肽敏感性是未知的。在一些实施方案中，不期望的微生物有机体的抗微生物肽敏感性是已知的，并且不期望的微生物有机体的身份是未知的。

一些实施方案的生产方法和微生物群落的抗微生物肽可以杀死一种或多种不期望的微生物有机体或者阻止一种或多种不期望的微生物有机体的繁殖。例如，不期望的微生物有机体可能缺乏抗微生物肽的免疫调节剂(如果微生物肽包含细菌素)，或者通过抗微生物肽的带电残基或水解酶活性可能破坏不期望的微生物有机体的细胞膜或细胞壁，引起孔形成，导致对不期望的微生物有机体的细胞毒性。

考虑了在一些实施方案的生产方法和微生物群落中，抗微生物肽可以是经工程化以表达抗微生物肽的期望的微生物有机体外源的。例如，抗微生物肽可以来自与期望的微生物有机体不同的菌株或物种的基因组，或者抗微生物肽可以是天然存在的抗微生物肽的变体，或者抗微生物肽可以是完全合成的。因此，在一些实施方案的生产方法或微生物群落中，抗微生物肽不被遗传修饰的期望的微生物有机体物种的野生型基因组编码。在一些实施方案的生产方法或微生物群落中，抗微生物肽是遗传修饰的微生物有机体外源的，或者抗微生物肽是合成的。

在一些实施方案的生产方法或微生物群落中，核酸编码两种或更多种不同的抗微生物肽。例如，核酸可包含至少2、3、4、5、6、7、8、9或10种抗微生物肽，包括任何两个所列值之间的范围，例如2-5、2-7、2-10、3-5、3-7、3-10、5-7或5-10种抗微生物肽。例如，单一核酸可以编码两种或更多种抗微生物肽，例如，核酸可以编码本文所述的多肽原。例如，两种或更多种不同的核酸可以一起编码抗微生物肽。例如，在一些实施方案的生产方法中，施用核酸包括施用编码不同抗微生物肽的两种或更多种不同的核酸。在一些实施方案的生产方法中，一起选择不同的抗微生物肽以靶向抗生素抗性感染(例如，多重耐药(MDR)细菌的感染)。

在一些实施方案的生产方法中，施用核酸包括施用包含本文所述核酸的质粒、染色体外阵列、附加体、微型染色体，或者施用包含核酸的噬菌体。在一些实施方案的生产方法中，施用核酸包括施用包含本文所述核酸的质粒，或者施用包含核酸的噬菌体。

考虑了经遗传修饰以表达一种或多种如本文所述的抗微生物肽的期望的微生物有机体可赋予群落中其它微生物有机体益处，例如通过使它们防御不期望的微生物有机体。因此，如果微生物群落中的一些，但不是所有期望的微生物有机体被遗传修饰以表达抗微生物肽，则微生物群落通常可以获得针对不期望的微生物有机体的保护的益处。在一些实施方案的生产方法中，期望的微生物有机体包含微生物有机体的两种或更多种不同物种，其中将核酸施用至所述不同物种中的仅一种或多于一种。

在一些实施方案的生产方法中，所述方法还包括将不同的核酸(其也可称为“第二核酸”)原位施用至微生物群落中不同于基因工程化的微生物有机体的微生物有机体(其也可称为“第二微生物有机体”)。不同的(或“第二”)核酸可以编码与核酸不同的抗微生物肽。例如，至少1、2、3、4、5、6、7、8、9、10种不同的核酸(包括任何两个所列值之间的范围，例如，1-5、1-7、1-10、2-5、2-7、2-10、3-7、3-10、5-7或5-10)，各自编码不同的抗微生物肽或抗微生物肽的组合。在一些实施方案的生产方法中，不同的核酸(或“第二核酸”)与核酸(或“第一核酸”)同时施用。在一些实施方案的生产方法中，在与核酸(或“第一核酸”)不同的时间和/或位置施用不同的核酸(或“第二核酸”)。在一些实施方案的生产方法中，在与核酸(或“第一核酸”)不同的时间施用不同的核酸(或“第二核酸”)。

考虑了根据本文所述的生产方法或微生物群落中的任一种的微生物群落可以由微生物组组成。例如，微生物组可以包含微生物群落，基本上由其组成，或由其组成。适用于本文所述实施方案的示例性微生物组包括选自以下的微生物组：胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤。还应注意，微生物群落可以在环境内，所述环境可以具有额外的特征，如温度、压力、湿度、pH等。根据本文所述的生产方法或微生物群落中的任一种的期望的微生物菌株可适于微生物组、微生物群落和/或环境。在一些实施方案的生产方法中，微生物群落由微生物组组成。

在一些实施方案的方法中，可以将核酸离体施用至期望的微生物有机体。例如，微生物群落可以是对象自体的，并且可以离体施用核酸。所述方法还可以包括将包含遗传修饰的期望的微生物有机体的微生物群落施用至对象。例如，包含微生物群落的样品可以获自对象的微生物组，可以将核酸离体施用至期望的微生物有机体，以及可以将包含遗传修饰的期望的微生物有机体的微生物群落施用至对象。任选地，在收集样品之后，可以除去或根除(例如，通过抗生素和/或抗微生物肽)内源性微生物组，并且可以将包含遗传修饰的期望的微生物有机体的微生物群落施用至对象以代替被除去或根除的内源性微生物组。在一些实施方案的生产方法或微生物群落中，微生物群落在对象的微生物组的保存的健康样品中。在施用时，对象可能患有其微生物组的失调。可以例如用抗生素和/或抗微生物肽除去或根除对象中的这种微生物组(患有失调)。然后可以将包含遗传修饰的期望的微生物有机体的微生物群落施用至对象。因此，保存的健康样品的微生物群落可以代替失调微生物组。

在一些实施方案的生产方法或微生物群落中，微生物群落由工业培养物组成。例如，工业培养物可以发酵物质以形成目的产物，或者可以降解废物或有毒物质。例如，工业培养物可以发酵原料，或者工业培养物可以用于制造药物或化妆品产品。

参考图3，显示了一些非限制性实施方案的生产方法的示意图。生产方法可以包括鉴定310期望的微生物有机体301，所述微生物有机体301是微生物群落300(例如微生物组或工业培养物中的微生物群落)的成员。所述方法可以包括将编码抗微生物肽304的核酸施用320至至少一种期望的微生物有机体302，并获得遗传修饰的期望的微生物有机体303。遗传修饰的期望的微生物有机体可以被配置成表达抗微生物肽。例如，遗传修饰的期望的微生物有机体可包含在本文所述启动子控制下的编码抗微生物肽的核酸。转到框330，生产方法可以包括遗传修饰的期望的微生物有机体在允许其生长时将抗微生物肽分泌到微生物群落环境中(330)。在一些实施方案中，生产方法不包括将任何微生物有机体加入到微生物群落中。

微生物群落

根据本文的一些实施方案，描述了微生物群落。微生物群落可以包含期望的微生物有机体。至少一种期望的微生物有机体可以被遗传修饰，包含第一核酸。第一核酸可编码不靶向一种或多种期望的微生物有机体的第一抗微生物肽。第一核酸还可以编码第一抗微生物肽框内的分泌信号。微生物群落还可以包含与第一核酸具有相同序列的无细胞的第二核酸。例如，微生物群落可以是，例如人或非人哺乳动物的微生物组的一部分，或者可以是工业培养物的一部分，例如发酵、或药物或化妆品制造培养物。例如，无细胞的第二核酸的存在可以是表明第一核酸原位施用至期望的微生物有机体(如本文所述)的结构。在一些实施方案的微生物群落中，无细胞的第二核酸由质粒、染色体外阵列、附加体、微型染色体或噬菌体组成。在一些实施方案中，无细胞的第二核酸以足以遗传修饰期望的微生物有机体的量存在于微生物群落中。在一些实施方案中，微生物群落处于足以促进期望的微生物有机体群体的生长或维持的条件下。

如本文所述，可有利地对微生物群落(和生产方法)的期望的微生物有机体进行遗传修饰以生产一种或多种不能内源性产生的抗微生物肽。这种遗传修饰可扩大期望的微生物有机体(和微生物群落)对不期望的微生物有机体如病原体或污染物的防御活性范围。因此，在一些实施方案中，第一抗微生物肽不被遗传修饰的期望的微生物有机体物种的野生型基因组编码。

考虑了许多抗微生物肽适用于本文所述的微生物群落和生产方法，例如本文所述的细菌素。典型的抗微生物肽的实例也如本文所述。在一些实施方案中，第一抗微生物肽是合成的。在一些实施方案的生产方法或微生物群落中，第一抗微生物肽是遗传修饰的微生物有机体外源的。

如本文所论述的，期望的微生物有机体可以通过表达抗微生物肽而赋予其自身和/或微生物群落益处，即使它不包含抗微生物肽的免疫调节剂亦如此。因此，在一些实施方案的生产方法或微生物群落中，包含编码抗微生物肽的核酸的期望的微生物有机体不包含编码抗微生物肽的免疫调节剂的核酸。

在一些实施方案的微生物群落(或生产方法)中，微生物群落被一种或多种未知身份的不期望的微生物有机体污染。例如，不期望的微生物有机体可包含病原体，或可污染微生物群落，或可干扰微生物群落对工业产品的发酵或生产。在一些实施方案中，不期望的微生物有机体的身份是已知的。在一些实施方案中，不期望的微生物有机体是微生物群落的常见病原体或污染物。在一些实施方案中，不期望的微生物有机体的抗微生物肽敏感性是已知的。在一些实施方案中，不期望的微生物有机体的抗微生物肽敏感性是未知的。在一些实施方案中，不期望的微生物有机体的抗微生物肽敏感性是已知的，并且不期望的微生物有机体的身份是未知的。

本文的一些实施方案的生产方法和微生物群落的期望的微生物肽可以被遗传修饰以编码和表达两种或更多种不同的抗微生物肽。两种或更多种不同的抗微生物肽可以包含具有选定的抗微生物活性谱的抗微生物肽的混合物。不同的抗微生物肽可以由相同的核酸或不同的核酸编码。在一些实施方案的微生物群落(或生产方法)中，第一核酸编码两种或更多种不同的抗微生物肽，例如至少2、3、4、5、6、7、8、9或10种不同的抗微生物肽，包括任何两个所列值之间的范围，例如2-5、2-7、2-10、3-5、3-7、3-10、5-7或5-10种抗微生物肽。在一些实施方案的微生物群落(或生产方法)中，遗传修饰的期望的微生物有机体还包含第三核酸，其编码不靶向一种或多种期望的微生物有机体的第二抗微生物肽和第二抗微生物肽框内的分泌信号。

在一些实施方案的微生物群落(或生产方法)中，微生物群落可以含有两种或更多种不同的期望的微生物有机体，其各自被遗传修饰以编码和表达一种或多种如本文所述的抗微生物肽。在一些实施方案的微生物群落(或生产方法)中，期望的微生物有机体包含微生物有机体的两种或更多种不同物种。例如，微生物有机体的两种或更多种不同物种可以各自包含编码相同抗微生物肽或不同抗微生物肽的核酸，因此可以各自表达抗微生物肽或不同的抗微生物肽。在一些实施方案的微生物群落(或生产方法)中，期望的微生物有机体包含微生物有机体的两种或更多种不同物种。第一核酸可仅由不同物种中的一种组成。在一些实施方案的微生物群落(或生产方法)中，期望的微生物有机体包含微生物有机体的两种或更多种不同物种。第一核酸可以由两种或更多种不同的物种组成。

根据一些实施方案，如本文所述的微生物群落由微生物组组成。例如，微生物组可以是选自以下的微生物组：胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤，包括所列值中的两个或更多个的组合。例如，微生物群落可以在对象体内原位(例如对象的微生物组的一部分)。可以将编码抗微生物肽的核酸体内施用至期望的微生物细胞。例如，编码抗微生物肽的核酸可以被配制用于体内递送至微生物组，如局部、口服或直肠施用。制剂技术的实例描述在Remington:The Science andPractice of Pharmacy(Allen,L.V.编辑,第22版,Pharmaceutical Press,Philadelphia,PA(2014))中，将其通过引用以其整体并入本文。

例如，微生物群落可以是对象自体的，并且可以是对象离体的。例如，如果对象的微生物组是共生的，并且离体的自体微生物组包含健康微生物组的化学计量的微生物物种和/或菌株，则这种微生物群落可用于重新引入或重新定殖对象的微生物组。

本文所用的“对象”可以是具有微生物群落的任何合适的对象，其中期望原位生产分泌的抗微生物肽。对象可以是哺乳动物、非人类哺乳动物或非哺乳动物对象。在一些实施方案中，对象是但不限于人类、非人类灵长类、鼠、牛、猪、马、猫、犬或禽类对象。在一些实施方案中，对象是驯养动物。

根据一些实施方案，如本文所述的微生物群落是工业培养物。例如，工业培养物可以用于发酵、废物的破坏或者制造药物或化妆品产品。

实施例

实施例1：在微生物组中的微生物群落中原位生产分泌的抗微生物肽

获得对象的肠道微生物组的样品，并通过16S测序进行分析以证实在对象的肠道微生物组中存在期望的微生物有机体。双歧杆菌属物种被确定为对象肠道微生物组中期望的微生物有机体。确定双歧杆菌属物种不被杀死并且其生长不被细菌素31抑制。制备用于双歧杆菌属物种的噬菌体以含有编码细菌素31的核酸。通过摄入将噬菌体施用至双歧杆菌属物种，并且通过噬菌体转导对对象肠道中的双歧杆菌属物种群体的一部分进行遗传修饰以表达细菌素31。肠道微生物组中遗传修饰的双歧杆菌属物种将细菌素31分泌到对象的肠道环境中。没有额外的双歧杆菌被加入到对象的肠道微生物组中，使得双歧杆菌物种与对象的肠道微生物组中的其它成员的比例没有改变。

实施例2：在工业发酵罐中的微生物群落中原位生产分泌的抗微生物肽

使酿酒酵母菌株在工业发酵罐中生长。已知这种酿酒酵母菌株对Leucococin C和Diversin V41不敏感。将编码Leucococin C和Diversin V41的质粒施用至发酵罐中的酿酒酵母细胞(例如通过转接合(transconjugation))。用质粒转化发酵罐中酿酒酵母的亚群，并将Leucococin C和Diversin V41分泌到发酵储液(fermentation stock)中。因此，对发酵罐中的酿酒酵母进行工程化以分泌抗微生物肽。

在本文所述的至少一些实施方案中，在实施方案中使用的一个或多个元件可以在另一个实施方案中互换使用，除非这样的替换在技术上不可行。本领域技术人员将理解，在不脱离所要求保护的主题的范围的情况下，可以对本文所述的方法和结构作出各种其它省略、添加和修改。所有这些修改和变化都旨在落入由所附权利要求限定的主题范围内。

关于本文中使用的实质上任何复数和/或单数术语，根据适合于上下文和/或应用，本领域技术人员可从复数转化成单数和/或从单数转化成复数。为了清楚起见，不同单数/复数变换可以在本文清楚地列出。

本领域技术人员将理解，通常，本文使用的术语，尤其是在所附权利要求(例如，所附权利要求的主体)中，通常旨在作为“开放”术语(例如，术语“包括(including)”应被理解为“包括但不限于”，术语“具有”应被理解为“至少具有”，术语“包括(includes)”应被理解为“包括但不限于”等)。本领域的技术人员还将理解，如果打算采用特定数量的引入权利要求叙述，则在权利要求中明确地叙述这种意图，在没有这种叙述的情况下，不存在这种意图。例如，为了帮助理解，以下所附权利要求可以包含使用介绍性短语“至少一个(种)”和“一个或多个/一种或多种”来引入权利要求叙述。然而，这种短语的使用不应被解释为暗示通过不定冠词“一个/一种(a)”或“一个/一种(an)”的权利要求叙述的引入将含有这种引入的权利要求陈述的任何特定权利要求限制为仅包含一个这种陈述的实施方案，即使当同一权利要求包括介绍性短语“一个或多个/一种或多种”或“至少一个(种)”和不定冠词如“一个/一种(a)”或“一个/一种(an)”时(例如“一个/一种(a)”和/或“一个/一种(an)”应被解释为意指至少一个(种)”或“一个或多个/一种或多种”)亦如此；对于用于引入权利要求叙述的定冠词的使用也是如此。另外，即使明确地叙述了具体数量的引入的权利要求叙述，本领域技术人员将认识到，这种叙述应被解释为意指至少所述的数量(例如，没有其它修饰语的“两个叙述”的基本叙述意指至少两个叙述或者两个或更多个叙述)。此外，在其中使用类似于“A、B和C中的至少一个等”的惯例的那些情况下，通常，这种结构旨在意为本领域技术人员将理解该惯例(例如，“具有A、B和C中的至少一个的系统”将包括但不限于具有单独的A、单独的B、单独的C、A和B一起、A和C一起、B和C一起、和/或A、B和C一起等的系统)。在其中使用类似于“A、B或C中的至少一个等”的惯例的那些情况下，通常，这种结构旨在意为本领域技术人员将理解该惯例(例如，“具有A、B或C中的至少一个的系统”将包括但不限于具有单独的A、单独的B、单独的C、A和B一起、A和C一起、B和C一起、和/或A、B和C一起等的系统)。本领域的技术人员还将理解，无论在说明书、权利要求书或附图中，呈现两个或更多个替代术语的几乎任何分离的词语(disjunctive word)和/或短语都应被理解为考虑包括这些术语中的一个、这些术语中的任一个或这两个术语的可能性。例如，短语“A或B”将被理解为包括“A”或“B”或“A和B”的可能性。

另外，在本公开内容的特征或方面按照马库什组(Markush group)描述的情况下，本领域技术人员将认识到本公开内容也按照马库什组中的任何单个成员或成员亚组来描述。

如本领域技术人员将理解的，出于任何和所有目的，如就提供书面描述而言，本文公开的所有范围还涵盖任何和所有可能的子范围及其子范围的组合。任何列出的范围可以容易地被认为充分地描述并且使得相同的范围能够被分成至少两等份、三等份、四等份、五等份、十等份等。作为非限制性实例，本文讨论的每个范围可以被容易地分成下三分之一，中三分之一和上三分之一等。如本领域技术人员还将理解的，所有语言如“至多”、“至少”、“大于”、“小于”等包括所叙述的数字，并且是指随后可被分成如本文所讨论的子范围的范围。最后，如本领域技术人员将理解的，范围包括每个单独的成员。因此，例如，具有1-3个制品的组是指具有1个、2个或3个制品的组。类似地，具有1-5个制品的组是指具有1个、2个、3个、4个或5个制品的组等。

虽然本文已经公开了各个方面和实施方案，但其它方面和实施方案对于本领域技术人员将是显而易见的。本文公开的各个方面和实施方案是为了说明的目的，而非旨在限制，其中真正的范围和精神由所附权利要求来指示。对于本文所述的每种方法，明确地预期了用于所述方法的相关组合物，也明确地预期了所述方法中的组合物的用途，以及如果适用，制备用于所述方法的药物的方法。例如，对于在包含抗微生物肽或编码抗微生物肽的核酸的微生物群落中原位生产分泌的抗微生物肽的方法，也考虑了用于相应方法中的抗微生物肽(和/或编码抗微生物肽的核酸)，以及根据所述方法抗微生物肽(和/或编码抗微生物肽的核酸)在微生物群落中表达和/或抑制未描述的微生物有机体的用途。还考虑了制备用于在微生物群落中表达和/或抑制未描述的微生物有机体的包含抗微生物肽(和/或编码抗微生物肽的核酸)的药物的方法。

序列表

<110> 辛格隆股份公司(Syngulon SA)

<120> 微生物群落中微生物的控制

<130> SYNG.007WO

<150> 63/112,084

<151> 2020-11-10

<160> 750

<170> FastSEQ for Windows Version 4.0

<210> 1

<211> 40

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 1

Trp Leu Pro Pro Ala Gly Leu Leu Gly Arg Cys Gly Arg Trp Phe Arg

1 5 10 15

Pro Trp Leu Leu Trp Leu Gln Ser Gly Ala Gln Tyr Lys Trp Leu Gly

20 25 30

Asn Leu Phe Gly Leu Gly Pro Lys

35 40

<210> 2

<211> 5

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> IIa类末端的N-末端基序

<220>

<221> VARIANT

<222> 3

<223> Xaa =任意氨基酸

<400> 2

Tyr Gly Xaa Gly Val

1 5

<210> 3

<211> 135

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 杂合细菌素Ent35-MccV

<400> 3

Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser

1 5 10 15

Val Asp Trp Gly Arg Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala

20 25 30

Asn Leu Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser Gly Gly Gly Ala

35 40 45

Ser Gly Arg Asp Ile Ala Met Ala Ile Gly Thr Leu Ser Gly Gln Phe

50 55 60

Val Ala Gly Gly Ile Gly Ala Ala Ala Gly Gly Val Ala Gly Gly Ala

65 70 75 80

Ile Tyr Asp Tyr Ala Ser Thr His Lys Pro Asn Pro Ala Met Ser Pro

85 90 95

Ser Gly Leu Gly Gly Thr Ile Lys Gln Lys Pro Glu Gly Ile Pro Ser

100 105 110

Glu Ala Trp Asn Tyr Ala Ala Gly Arg Leu Cys Asn Trp Ser Pro Asn

115 120 125

Asn Leu Ser Asp Val Cys Leu

130 135

<210> 4

<211> 46

<212> PRT

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 4

Met Ile Ser Ser His Gln Lys Thr Leu Thr Asp Lys Glu Leu Ala Leu

1 5 10 15

Ile Ser Gly Gly Lys Thr His Tyr Pro Thr Asn Ala Trp Lys Ser Leu

20 25 30

Trp Lys Gly Phe Trp Glu Ser Leu Arg Tyr Thr Asp Gly Phe

35 40 45

<210> 5

<211> 141

<212> DNA

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 5

atgatttcat ctcatcaaaa aacgttaact gataaagaat tagcattaat ttctgggggg 60

aaaacgcact acccgactaa tgcatggaaa agtctttgga aaggtttctg ggaaagcctt 120

cgttatactg acggttttta g 141

<210> 6

<211> 81

<212> PRT

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 6

Met Ile Ser Met Ile Ser Ser His Gln Lys Thr Leu Thr Asp Lys Glu

1 5 10 15

Leu Ala Leu Ile Ser Gly Gly Lys Thr Tyr Tyr Gly Thr Asn Gly Val

20 25 30

His Cys Thr Lys Lys Ser Leu Trp Gly Lys Val Arg Leu Lys Asn Val

35 40 45

Ile Pro Gly Thr Leu Cys Arg Lys Gln Ser Leu Pro Ile Lys Gln Asp

50 55 60

Leu Lys Ile Leu Leu Gly Trp Ala Thr Gly Ala Phe Gly Lys Thr Phe

65 70 75 80

His

<210> 7

<211> 246

<212> DNA

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 7

atgatttcaa tgatttcatc tcatcaaaaa acgttaactg ataaagaatt agcattaatt 60

tctgggggga aaacgtacta tggtactaat ggtgtgcatt gtactaaaaa gagtctttgg 120

ggtaaagtac gcttaaaaaa cgtgattcct ggaactcttt gtcgtaagca atcgttgccg 180

atcaaacagg atttaaaaat tttactgggc tgggctacag gtgcttttgg caagacattt 240

cattaa 246

<210> 8

<211> 60

<212> PRT

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 8

Met Asp Lys Lys Thr Lys Ile Leu Phe Glu Val Leu Tyr Ile Ile Cys

1 5 10 15

Ile Ile Gly Pro Gln Phe Ile Leu Phe Val Thr Ala Lys Asn Asn Met

20 25 30

Tyr Gln Leu Val Gly Ser Phe Val Gly Ile Val Trp Phe Ser Tyr Ile

35 40 45

Phe Trp Tyr Ile Phe Phe Lys Gln His Lys Lys Met

50 55 60

<210> 9

<211> 183

<212> DNA

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 9

atggataaga aaacaaaaat attatttgaa gtattataca tcatctgtat aataggccct 60

caatttatat tatttgtgac tgcaaaaaac aatatgtatc agttggtggg ttcgtttgtt 120

ggaatagtat ggttttcgta tattttttgg tatatttttt tcaaacaaca taaaaaaatg 180

tag 183

<210> 10

<211> 65

<212> PRT

<213> 格氏乳杆菌(Lactobacillus gasseri)

<400> 10

Met Ala Leu Lys Thr Leu Glu Lys His Glu Leu Arg Asn Val Met Gly

1 5 10 15

Gly Asn Lys Trp Gly Asn Ala Val Ile Gly Ala Ala Thr Gly Ala Thr

20 25 30

Arg Gly Val Ser Trp Cys Arg Gly Phe Gly Pro Trp Gly Met Thr Ala

35 40 45

Cys Ala Leu Gly Gly Ala Ala Ile Gly Gly Tyr Leu Gly Tyr Lys Ser

50 55 60

Asn

65

<210> 11

<211> 198

<212> DNA

<213> 格氏乳杆菌(Lactobacillus gasseri)

<400> 11

atggctttaa aaacattaga aaaacatgaa ttaagaaatg taatgggtgg aaacaagtgg 60

gggaatgctg taataggagc tgctacggga gctactcgcg gagtaagttg gtgcagagga 120

ttcggaccat ggggaatgac tgcctgtgcg ttaggaggtg ctgcaattgg aggatatctg 180

ggatataaga gtaattaa 198

<210> 12

<211> 51

<212> PRT

<213> 金黄色葡萄球菌(Staphylococcus aureus)

<400> 12

Met Ser Trp Leu Asn Phe Leu Lys Tyr Ile Ala Lys Tyr Gly Lys Lys

1 5 10 15

Ala Val Ser Ala Ala Trp Lys Tyr Lys Gly Lys Val Leu Glu Trp Leu

20 25 30

Asn Val Gly Pro Thr Leu Glu Trp Val Trp Gln Lys Leu Lys Lys Ile

35 40 45

Ala Gly Leu

50

<210> 13

<211> 156

<212> DNA

<213> 金黄色葡萄球菌(Staphylococcus aureus)

<400> 13

atgagttggt taaatttttt aaaatacatc gctaaatatg gcaaaaaagc ggtatctgct 60

gcttggaagt acaaaggtaa agtattagaa tggcttaatg ttggtcctac tcttgaatgg 120

gtatggcaaa aattaaagaa aattgctgga ttataa 156

<210> 14

<211> 61

<212> PRT

<213> 鸟肠球菌(Enterococcus avium)

<400> 14

Met Thr Arg Ser Lys Lys Leu Asn Leu Arg Glu Met Lys Asn Val Val

1 5 10 15

Gly Gly Thr Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys

20 25 30

Ser Val Asp Trp Gly Lys Ala Ile Ser Ile Ile Gly Asn Asn Ser Ala

35 40 45

Ala Asn Leu Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser

50 55 60

<210> 15

<211> 186

<212> DNA

<213> 鸟肠球菌(Enterococcus avium)

<400> 15

atgacaagat caaaaaaatt aaatttacgc gaaatgaaga atgttgttgg tggtacctac 60

tatggaaatg gtgtatcttg taacaagaaa ggctgttcag ttgactgggg caaagccatc 120

agtattatag gaaataattc cgcagcaaac ttagcaactg gtggtgctgc tggttggaag 180

tcataa 186

<210> 16

<211> 67

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 16

Met Lys Lys Lys Leu Val Ile Cys Gly Ile Ile Gly Ile Gly Phe Thr

1 5 10 15

Ala Leu Gly Thr Asn Val Glu Ala Ala Thr Tyr Tyr Gly Asn Gly Leu

20 25 30

Tyr Cys Asn Lys Gln Lys Cys Trp Val Asp Trp Asn Lys Ala Ser Arg

35 40 45

Glu Ile Gly Lys Ile Ile Val Asn Gly Trp Val Gln His Gly Pro Trp

50 55 60

Ala Pro Arg

65

<210> 17

<211> 204

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 17

atgaaaaaga aattagttat ttgtggcatt attgggattg gttttacagc attaggaaca 60

aatgtagaag ctgctacgta ttacggaaat ggtttatatt gtaataagca aaaatgttgg 120

gtagactgga ataaagcttc aagggaaatt ggaaaaatta ttgttaatgg ttgggtacaa 180

catggccctt gggctcctag atag 204

<210> 18

<211> 51

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 18

Met Lys Glu Gln Asn Ser Phe Asn Leu Leu Gln Glu Val Thr Glu Ser

1 5 10 15

Glu Leu Asp Leu Ile Leu Gly Ala Lys Gly Gly Ser Gly Val Ile His

20 25 30

Thr Ile Ser His Glu Val Ile Tyr Asn Ser Trp Asn Phe Val Phe Thr

35 40 45

Cys Cys Ser

50

<210> 19

<211> 156

<212> DNA

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 19

atgaaagaac aaaactcttt taatcttctt caagaagtga cagaaagtga attggacctt 60

attttaggtg caaaaggcgg cagtggagtt attcatacaa tttctcatga agtaatatat 120

aatagctgga actttgtatt tacttgctgc tcttaa 156

<210> 20

<211> 74

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 20

Met Lys Lys Lys Val Leu Lys His Cys Val Ile Leu Gly Ile Leu Gly

1 5 10 15

Thr Cys Leu Ala Gly Ile Gly Thr Gly Ile Lys Val Asp Ala Ala Thr

20 25 30

Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Glu Lys Cys Trp Val Asp

35 40 45

Trp Asn Gln Ala Lys Gly Glu Ile Gly Lys Ile Ile Val Asn Gly Trp

50 55 60

Val Asn His Gly Pro Trp Ala Pro Arg Arg

65 70

<210> 21

<211> 225

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 21

atgaaaaaga aagtattaaa acattgtgtt attctaggaa tattaggaac ttgtctagct 60

ggcatcggta caggaataaa agttgatgca gctacttact atggaaatgg tctttattgt 120

aacaaagaaa aatgttgggt agattggaat caagctaaag gagaaattgg aaaaattatt 180

gttaatggtt gggttaatca tggtccatgg gcacctagaa ggtag 225

<210> 22

<211> 50

<212> PRT

<213> 肉毒杆菌(Clostridium botulinum)

<400> 22

Met Gln Lys Pro Glu Ile Ile Ser Ala Asp Leu Gly Leu Cys Ala Val

1 5 10 15

Asn Glu Phe Val Ala Leu Ala Ala Ile Pro Gly Gly Ala Ala Thr Phe

20 25 30

Ala Val Cys Gln Met Pro Asn Leu Asp Glu Ile Val Ser Asn Ala Ala

35 40 45

Tyr Val

50

<210> 23

<211> 153

<212> DNA

<213> 肉毒杆菌(Clostridium botulinum)

<400> 23

atgcaaaaac cagaaattat tagtgctgat ttagggcttt gtgcagttaa tgaatttgta 60

gctcttgctg ccattcctgg tggtgctgct acatttgcag tatgccaaat gccaaacttg 120

gatgagattg ttagtaatgc agcatatgtt taa 153

<210> 24

<211> 58

<212> PRT

<213> 马链球菌(Streptococcus equinus)

<400> 24

Met Met Asn Ala Thr Glu Asn Gln Ile Phe Val Glu Thr Val Ser Asp

1 5 10 15

Gln Glu Leu Glu Met Leu Ile Gly Gly Ala Asp Arg Gly Trp Ile Lys

20 25 30

Thr Leu Thr Lys Asp Cys Pro Asn Val Ile Ser Ser Ile Cys Ala Gly

35 40 45

Thr Ile Ile Thr Ala Cys Lys Asn Cys Ala

50 55

<210> 25

<211> 177

<212> DNA

<213> 马链球菌(Streptococcus equinus)

<400> 25

atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa 60

atgttaattg gtggtgcaga tcgtggatgg attaagactt taacaaaaga ttgtccaaat 120

gtaatttctt caatttgtgc aggtacaatt attacagctt gtaaaaattg tgcttaa 177

<210> 26

<211> 77

<212> PRT

<213> 田野环丝菌(Brochothrix campestris)

<400> 26

Met His Lys Val Lys Lys Leu Asn Asn Gln Glu Leu Gln Gln Ile Val

1 5 10 15

Gly Gly Tyr Ser Ser Lys Asp Cys Leu Lys Asp Ile Gly Lys Gly Ile

20 25 30

Gly Ala Gly Thr Val Ala Gly Ala Ala Gly Gly Gly Leu Ala Ala Gly

35 40 45

Leu Gly Ala Ile Pro Gly Ala Phe Val Gly Ala His Phe Gly Val Ile

50 55 60

Gly Gly Ser Ala Ala Cys Ile Gly Gly Leu Leu Gly Asn

65 70 75

<210> 27

<211> 234

<212> DNA

<213> 田野环丝菌(Brochothrix campestris)

<400> 27

atgcacaagg taaaaaaatt aaacaatcaa gagttacaac agatcgtggg aggttacagt 60

tcaaaagatt gtctaaaaga tattggtaaa ggaattggtg ctggtacagt agctggggca 120

gccggcggtg gcctagctgc aggattaggt gctatcccag gagcattcgt tggagcacat 180

tttggagtaa tcggcggatc tgccgcatgc attggtggat tattaggtaa ctag 234

<210> 28

<211> 80

<212> PRT

<213> 溶纤维丁酸弧菌(溶纤维丁酸弧菌(Butyrivibrio fibrisolvens))

<400> 28

Met Ser Lys Lys Gln Ile Met Ser Asn Cys Ile Ser Ile Ala Leu Leu

1 5 10 15

Ile Ala Leu Ile Pro Asn Ile Tyr Phe Ile Ala Asp Lys Met Gly Ile

20 25 30

Gln Leu Ala Pro Ala Trp Tyr Gln Asp Ile Val Asn Trp Val Ser Ala

35 40 45

Gly Gly Thr Leu Thr Thr Gly Phe Ala Ile Ile Val Gly Val Thr Val

50 55 60

Pro Ala Trp Ile Ala Glu Ala Ala Ala Ala Phe Gly Ile Ala Ser Ala

65 70 75 80

<210> 29

<211> 243

<212> DNA

<213> 溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)

<400> 29

atgagtaaaa aacaaattat gagtaactgt atatcaattg cattattaat agcactaatt 60

cctaatatct attttattgc agataaaatg ggaattcagt tagcacctgc ttggtatcaa 120

gatattgtga attgggtatc tgctggtgga acacttacta ctggttttgc gattattgta 180

ggagttacag taccggcatg gatagcagaa gcagctgcag cttttggtat agcttcagca 240

tga 243

<210> 30

<211> 48

<212> PRT

<213> 溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)

<400> 30

Met Asn Lys Glu Leu Asn Ala Leu Thr Asn Pro Ile Asp Glu Lys Glu

1 5 10 15

Leu Glu Gln Ile Leu Gly Gly Gly Asn Gly Val Ile Lys Thr Ile Ser

20 25 30

His Glu Cys His Met Asn Thr Trp Gln Phe Ile Phe Thr Cys Cys Ser

35 40 45

<210> 31

<211> 147

<212> DNA

<213> 溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)

<400> 31

atgaacaaag aacttaatgc acttacaaat cctattgacg agaaggagct tgagcagatc 60

ctcggtggtg gcaatggtgt catcaagaca atcagccacg agtgccacat gaacacatgg 120

cagttcattt tcacatgttg ctcttaa 147

<210> 32

<211> 66

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 32

Met Asn Ser Val Lys Glu Leu Asn Val Lys Glu Met Lys Gln Leu His

1 5 10 15

Gly Gly Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys

20 25 30

Ser Val Asn Trp Gly Gln Ala Phe Gln Glu Arg Tyr Thr Ala Gly Ile

35 40 45

Asn Ser Phe Val Ser Gly Val Ala Ser Gly Ala Gly Ser Ile Gly Arg

50 55 60

Arg Pro

65

<210> 33

<211> 201

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 33

atgaatagcg taaaagaatt aaacgtgaaa gaaatgaaac aattacacgg tggagtaaat 60

tatggtaatg gtgtttcttg cagtaaaaca aaatgttcag ttaactgggg acaagccttt 120

caagaaagat acacagctgg aattaactca tttgtaagtg gagtcgcttc tggggcagga 180

tccattggta ggagaccgta a 201

<210> 34

<211> 61

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 34

Met Lys Ser Val Lys Glu Leu Asn Lys Lys Glu Met Gln Gln Ile Asn

1 5 10 15

Gly Gly Ala Ile Ser Tyr Gly Asn Gly Val Tyr Cys Asn Lys Glu Lys

20 25 30

Cys Trp Val Asn Lys Ala Glu Asn Lys Gln Ala Ile Thr Gly Ile Val

35 40 45

Ile Gly Gly Trp Ala Ser Ser Leu Ala Gly Met Gly His

50 55 60

<210> 35

<211> 186

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 35

atgaaaagcg ttaaagaact aaataaaaaa gaaatgcaac aaattaatgg tggagctatc 60

tcttatggca atggtgttta ttgtaacaaa gagaaatgtt gggtaaacaa ggcagaaaac 120

aaacaagcta ttactggaat agttatcggt ggatgggctt ctagtttagc aggaatggga 180

cattaa 186

<210> 36

<211> 71

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 36

Met Asn Asn Val Lys Glu Leu Ser Ile Lys Glu Met Gln Gln Val Thr

1 5 10 15

Gly Gly Asp Gln Met Ser Asp Gly Val Asn Tyr Gly Lys Gly Ser Ser

20 25 30

Leu Ser Lys Gly Gly Ala Lys Cys Gly Leu Gly Ile Val Gly Gly Leu

35 40 45

Ala Thr Ile Pro Ser Gly Pro Leu Gly Trp Leu Ala Gly Ala Ala Gly

50 55 60

Val Ile Asn Ser Cys Met Lys

65 70

<210> 37

<211> 216

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 37

atgaataatg taaaagagtt aagtattaaa gaaatgcaac aagttactgg tggagaccaa 60

atgtcagatg gtgtaaatta tggaaaaggc tctagcttat caaaaggtgg tgccaaatgt 120

ggtttaggga tcgtcggcgg attagctact atcccttcag gtcctttagg ctggttagcc 180

ggagcagcag gtgtaattaa tagctgtatg aaataa 216

<210> 38

<211> 64

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 38

Met Leu Tyr Glu Leu Val Ala Tyr Gly Ile Ala Gln Gly Thr Ala Glu

1 5 10 15

Lys Val Val Ser Leu Ile Asn Ala Gly Leu Thr Val Gly Ser Ile Ile

20 25 30

Ser Ile Leu Gly Gly Val Thr Val Gly Leu Ser Gly Val Phe Thr Ala

35 40 45

Val Lys Ala Ala Ile Ala Lys Gln Gly Ile Lys Lys Ala Ile Gln Leu

50 55 60

<210> 39

<211> 195

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 39

atgttatatg aattagttgc atatggtatc gcacaaggta cagctgaaaa ggttgtaagt 60

ctaattaacg caggtttaac agtagggtct attatttcaa ttttgggtgg ggtcacagtc 120

ggtttatcag gtgtcttcac agcagttaaa gcagcaattg ctaaacaagg aataaaaaaa 180

gcaattcaat tataa 195

<210> 40

<211> 836

<212> PRT

<213> 胡萝卜软腐果胶杆菌胡萝卜亚种(Pectobacterium carotovorum subsp.Carotovorum)

<400> 40

Met Ile Lys Tyr Arg Leu Tyr Ala Pro Asn Asp Gly Asp Thr Met Thr

1 5 10 15

Val Ser Gly Gly Gly Gly Trp Val Ser Asn Asp Asp Arg Lys Gly Gly

20 25 30

Asn Asp Arg Asp Asn Gly Lys Gly Gly Ser Ala Val Asp Phe Ser Lys

35 40 45

Asn Pro Glu Lys Gln Ala Ile Val Asn Pro Tyr Leu Ala Ile Ala Ile

50 55 60

Pro Met Pro Val Tyr Pro Leu Tyr Gly Lys Leu Gly Phe Thr Ile Asn

65 70 75 80

Thr Thr Ala Ile Glu Thr Glu Leu Ala Asn Val Arg Ala Ala Ile Asn

85 90 95

Thr Lys Leu Ala Thr Leu Ser Ala Val Ile Gly Arg Ser Leu Pro Val

100 105 110

Val Gly Arg Val Phe Gly Val Thr Ala Ala Gly Met Trp Pro Ser Ser

115 120 125

Thr Ala Pro Ser Ser Leu Asp Ser Ile Tyr Asn Gln Ala His Gln Gln

130 135 140

Ala Leu Ala Gln Leu Ala Ala Gln Gln Gly Val Leu Asn Lys Gly Tyr

145 150 155 160

Asn Val Thr Ala Met Pro Ala Gly Phe Val Ser Ser Leu Pro Val Ser

165 170 175

Glu Ile Lys Ser Leu Pro Thr Ala Pro Ala Ser Leu Leu Ala Gln Ser

180 185 190

Val Ile Asn Thr Glu Leu Ser Gln Arg Gln Leu Ala Leu Thr Gln Pro

195 200 205

Thr Thr Asn Ala Pro Val Ala Asn Ile Pro Val Val Lys Ala Glu Lys

210 215 220

Thr Ala Met Pro Gly Val Tyr Ser Ala Lys Ile Ile Ala Gly Glu Pro

225 230 235 240

Ala Phe Gln Ile Lys Val Asp Asn Thr Lys Pro Ala Leu Ala Gln Asn

245 250 255

Pro Pro Lys Val Lys Asp Asp Ile Gln Val Ser Ser Phe Leu Ser Ser

260 265 270

Pro Val Ala Asp Thr His His Ala Phe Ile Asp Phe Gly Ser Asp His

275 280 285

Glu Pro Val Tyr Val Ser Leu Ser Lys Ile Val Thr Ala Glu Glu Glu

290 295 300

Lys Lys Gln Val Glu Glu Ala Lys Arg Arg Glu Gln Glu Trp Leu Leu

305 310 315 320

Arg His Pro Ile Thr Ala Ala Glu Arg Lys Leu Thr Glu Ile Arg Gln

325 330 335

Val Ile Ser Phe Ala Gln Gln Leu Lys Glu Ser Ser Val Ala Thr Ile

340 345 350

Ser Glu Lys Thr Lys Thr Val Ala Val Tyr Gln Glu Gln Val Asn Thr

355 360 365

Ala Ala Lys Asn Arg Asp Asn Phe Tyr Asn Gln Asn Arg Gly Leu Leu

370 375 380

Ser Ala Gly Ile Thr Gly Gly Pro Gly Tyr Pro Ile Tyr Leu Ala Leu

385 390 395 400

Trp Gln Thr Met Asn Asn Phe His Gln Ala Tyr Phe Arg Ala Asn Asn

405 410 415

Ala Leu Glu Gln Glu Ser His Val Leu Asn Leu Ala Arg Ser Asp Leu

420 425 430

Ala Lys Ala Glu Gln Leu Leu Ala Glu Asn Asn Arg Leu Gln Val Glu

435 440 445

Thr Glu Arg Thr Leu Ala Glu Glu Lys Glu Ile Lys Arg Asn Arg Val

450 455 460

Asn Val Ser Thr Phe Gly Thr Val Gln Thr Gln Leu Ser Lys Leu Leu

465 470 475 480

Ser Asp Phe Tyr Ala Val Thr Ser Leu Ser Gln Ser Val Pro Ser Gly

485 490 495

Ala Leu Ala Ser Phe Ser Tyr Asn Pro Gln Gly Met Ile Gly Ser Gly

500 505 510

Lys Ile Val Gly Lys Asp Val Asp Val Leu Phe Ser Ile Pro Val Lys

515 520 525

Asp Ile Pro Gly Tyr Lys Ser Pro Ile Asn Leu Asp Asp Leu Ala Lys

530 535 540

Lys Asn Gly Ser Leu Asp Leu Pro Ile Arg Leu Ala Phe Ser Asp Glu

545 550 555 560

Asn Gly Glu Arg Val Leu Arg Ala Phe Lys Ala Asp Ser Leu Arg Ile

565 570 575

Pro Ser Ser Val Arg Gly Val Ala Gly Ser Tyr Asp Lys Asn Thr Gly

580 585 590

Ile Phe Ser Ala Glu Ile Asp Gly Val Ser Ser Arg Leu Val Leu Glu

595 600 605

Asn Pro Ala Phe Pro Pro Thr Gly Asn Val Gly Asn Thr Gly Asn Thr

610 615 620

Ala Pro Asp Tyr Lys Ala Leu Leu Asn Thr Gly Val Asp Val Lys Pro

625 630 635 640

Val Asp Lys Ile Thr Val Thr Val Thr Pro Val Ala Asp Pro Val Asp

645 650 655

Ile Asp Asp Tyr Ile Ile Trp Leu Pro Thr Ala Ser Gly Ser Gly Val

660 665 670

Glu Pro Ile Tyr Val Val Phe Asn Ser Asn Pro Tyr Gly Gly Thr Glu

675 680 685

Lys Gly Lys Tyr Ser Lys Arg Tyr Tyr Asn Pro Asp Lys Ala Gly Gly

690 695 700

Pro Ile Leu Glu Leu Asp Trp Lys Asn Val Lys Ile Asp His Ala Gly

705 710 715 720

Val Asp Asn Val Lys Leu His Thr Gly Arg Phe Lys Ala Ser Val Glu

725 730 735

Asn Lys Val Met Ile Glu Arg Leu Glu Asn Ile Leu Asn Gly Gln Ile

740 745 750

Thr Ala Thr Asp Thr Asp Lys Arg Phe Tyr Thr His Glu Leu Arg Glu

755 760 765

Leu Asn Arg Tyr Arg Asn Leu Gly Ile Lys Asp Gly Glu Val Pro Ser

770 775 780

Ser Ile Gln Glu Glu Ser Ala Val Trp Asn Asp Thr His Thr Ala Thr

785 790 795 800

Leu Glu Asp Tyr Lys Ile Asn Glu Lys Glu Gln Pro Leu Tyr Thr Asp

805 810 815

Ala Ala Leu Gln Ala Ala Tyr Glu Gln Glu Leu Lys Asp Ala Leu Gly

820 825 830

Gly Lys His Gly

835

<210> 41

<211> 2511

<212> DNA

<213> 胡萝卜软腐果胶杆菌胡萝卜亚种(Pectobacterium carotovorum subsp.Carotovorum)

<400> 41

atgattaaat accgtttata tgctccaaat gatggagaca ccatgacagt gagtggtggt 60

ggtggttggg tttcaaacga tgatcgcaaa ggtggtaatg acagggacaa tggcaaaggt 120

ggttctgccg ttgattttag taaaaatcca gaaaagcagg ctatcgttaa tccctatttg 180

gcaatcgcga taccgatgcc ggtctaccct ctttatggaa agctagggtt cacaataaat 240

acgacggcaa ttgagactga actcgcaaat gtcagagcag caattaacac taaacttgca 300

acactcagtg cagtgattgg cagatcactt ccggtcgttg ggcgggtatt tggtgttact 360

gccgccggaa tgtggccttc tagtaccgct cccagtagtc tcgattctat atacaatcaa 420

gcacatcagc aggctttagc ccagttagct gctcaacagg gagtattaaa taaagggtat 480

aacgttacag caatgcctgc aggtttcgtc agcagtttgc ctgttagtga aatcaaatca 540

ttgccaacag ctcccgccag tttactggca caaagtgtga ttaataccga actttcccag 600

cgtcaactgg ctcttactca gcccacgacg aatgcaccag tcgcgaatat tcccgtagtt 660

aaagcagaga aaacagcaat gccaggtgtg tattcagcga aaattattgc tggtgagcct 720

gcattccaaa tcaaggtcga taataccaaa cctgctttgg cacagaatcc gccgaaagta 780

aaagatgata ttcaggtatc ttctttcctt tcctcgccag tagctgatac gcaccatgca 840

tttattgatt ttggcagcga tcatgaaccg gtatacgtgt ctctttcaaa gatcgtgaca 900

gccgaggagg agaaaaaaca ggttgaagag gccaagcgcc gtgagcagga gtggttgttg 960

cgtcatccaa ttacagctgc ggagcgaaaa ttaactgaaa tccgccaagt gatctctttt 1020

gctcaacagc taaaagaaag ctctgtcgca accatttcag aaaaaactaa aactgttgcg 1080

gtttaccaag aacaggtgaa taccgctgca aaaaatcgcg acaattttta taatcaaaat 1140

agaggtctgt taagtgcggg tataactggg ggaccgggat atcctattta tcttgcttta 1200

tggcaaacga tgaataactt tcatcaggct tatttcagag caaataatgc attggaacaa 1260

gagagtcatg ttctgaacct ggctcgttct gatctggcta aggctgagca attgcttgct 1320

gagaataatc gacttcaggt tgaaacggag cgaacgcttg ccgaagaaaa agagataaaa 1380

cgcaacaggg ttaatgtatc aacatttggc acagtgcaaa ctcaacttag taaattgctg 1440

tcagattttt atgctgttac atcactttcc caaagtgttc cttcgggggc attagcctct 1500

ttttcatata atccacaagg gatgattggc agcggtaaga ttgttgggaa ggatgtcgat 1560

gttttatttt ccatcccagt aaaagatatt ccgggatata aatctcctat taacttggac 1620

gatttagcca agaaaaatgg aagtctggat cttcccattc gtctggcatt ttctgatgag 1680

aatggagaaa gggttcttcg ggcattcaaa gcggatagtc tgcgaatccc ttcgagtgtc 1740

agaggtgtag cgggcagtta tgacaaaaat acgggtattt ttagtgcaga aattgatggt 1800

gtttcatctc gccttgtact ggaaaaccca gcgtttcctc cgaccggaaa tgtcggtaat 1860

acgggtaata ctgcacctga ctataaagca ttactgaata ctggtgttga tgttaaacct 1920

gttgataaaa tcacagttac ggtaacacca gttgctgatc cagtggatat tgatgactat 1980

ataatctggt tgccaactgc gtctggttct ggcgtggaac ccatttatgt cgtgtttaac 2040

agtaatccgt atggtgggac ggaaaaagga aaatatagca aacgttatta taatccagat 2100

aaggcaggcg gtccgatctt ggagctggat tggaaaaacg ttaagattga ccatgcaggt 2160

gtggacaatg ttaaattaca cacagggcgt ttcaaagcgt cggttgaaaa caaagtgatg 2220

attgaacgtt tggaaaacat actgaatggt caaatcacgg ccacggatac tgacaagcga 2280

ttctatacgc atgaattaag agagttaaac cgctacagaa atttaggcat caaagacggt 2340

gaagtgccta gtagcattca agaagaaagc gctgtttgga acgacacaca cacagcgacg 2400

cttgaagact acaaaattaa tgagaaagag caaccgttgt acactgatgc tgctttgcag 2460

gcagcctacg aacaggaact caaagacgca ttaggaggga aacatggcta a 2511

<210> 42

<211> 74

<212> PRT

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 42

Met Glu Asn Leu Gln Met Leu Thr Glu Glu Glu Leu Met Glu Ile Glu

1 5 10 15

Gly Gly Gly Trp Trp Asn Ser Trp Gly Lys Cys Val Ala Gly Thr Ile

20 25 30

Gly Gly Ala Gly Thr Gly Gly Leu Gly Gly Ala Ala Ala Gly Ser Ala

35 40 45

Val Pro Val Ile Gly Thr Gly Ile Gly Gly Ala Ile Gly Gly Val Ser

50 55 60

Gly Gly Leu Thr Gly Ala Ala Thr Phe Cys

65 70

<210> 43

<211> 225

<212> DNA

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 43

atggaaaact tacaaatgtt aactgaagaa gaattaatgg aaattgaagg tggaggctgg 60

tggaatagct ggggtaaatg tgttgctgga actatcggtg gagctggaac tggtggttta 120

ggtggagctg ctgcaggttc agctgttccg gttattggta ctggtattgg tggcgctatt 180

ggtggagtta gcggtggcct tacaggtgca gctacttttt gctaa 225

<210> 44

<211> 78

<212> PRT

<213> 灰轮丝链轮丝菌(Streptoverticillium griseoverticillatum)

<400> 44

Met Thr Ala Ser Ile Leu Gln Gln Ser Val Val Asp Ala Asp Phe Arg

1 5 10 15

Ala Ala Leu Leu Glu Asn Pro Ala Ala Phe Gly Ala Ser Ala Ala Ala

20 25 30

Leu Pro Thr Pro Val Glu Ala Gln Asp Gln Ala Ser Leu Asp Phe Trp

35 40 45

Thr Lys Asp Ile Ala Ala Thr Glu Ala Phe Ala Cys Arg Gln Ser Cys

50 55 60

Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly Asn Thr Lys

65 70 75

<210> 45

<211> 237

<212> DNA

<213> 灰轮丝链轮丝菌(Streptoverticillium griseoverticillatum)

<400> 45

atgaccgctt ccattcttca gcagtccgtc gtggacgccg acttccgcgc ggcgctgctt 60

gagaaccccg ccgccttcgg cgcttccgcc gcggccctgc ccacgcccgt cgaggcccag 120

gaccaggcgt cccttgactt ctggaccaag gacatcgccg ccacggaagc cttcgcctgc 180

cgccagagct gcagcttcgg cccgttcacc ttcgtgtgcg acggcaacac caagtaa 237

<210> 46

<211> 76

<212> PRT

<213> 嗜热地芽胞杆菌(Geobacillus kaustophilus)

<400> 46

Met Ser Leu Leu Ala Leu Val Ala Gly Thr Leu Gly Val Ser Gln Ser

1 5 10 15

Ile Ala Thr Thr Val Val Ser Ile Val Leu Thr Gly Ser Thr Leu Ile

20 25 30

Ser Ile Ile Leu Gly Ile Thr Ala Ile Leu Ser Gly Gly Val Asp Ala

35 40 45

Ile Leu Glu Ile Gly Trp Ser Ala Phe Val Ala Thr Val Lys Lys Ile

50 55 60

Val Ala Glu Arg Gly Lys Ala Ala Ala Ile Ala Trp

65 70 75

<210> 47

<211> 231

<212> DNA

<213> 嗜热地芽胞杆菌(Geobacillus kaustophilus)

<400> 47

atgagtttgc tggcgcttgt tgccgggacg ctcggcgtgt cacagtcaat cgcgacgacg 60

gttgtttcga ttgtgttgac cggctccact ctcatttcta ttattcttgg gatcaccgct 120

attttgtcag gtggagtcga cgccattttg gaaattgggt ggtcagcttt tgtcgcgacg 180

gtgaaaaaaa tagtggcgga acgaggaaaa gcggcagcga ttgcatggta a 231

<210> 48

<211> 309

<212> PRT

<213> 酪丁酸梭菌(Clostridium tyrobutyricum)

<400> 48

Met Arg Lys Val Phe Leu Arg Ser Ile Ile Ser Thr Leu Val Met Cys

1 5 10 15

Ala Phe Val Ser Ser Ser Phe Ser Val Asn Ala Asp Glu Ser Lys Pro

20 25 30

Asn Asp Glu Lys Ile Ile Asn Asn Ile Glu Asn Val Thr Thr Thr Lys

35 40 45

Asp Ile Val Lys Ser Asn Lys Asn Asn Ile Val Tyr Leu Asp Glu Gly

50 55 60

Val Met Ser Ile Pro Leu Ser Gly Arg Lys Pro Ile Ala Ile Lys Asp

65 70 75 80

Asp Asn Asn Lys Glu Asp Leu Thr Val Thr Leu Pro Ile Lys Asn Thr

85 90 95

Gly Asp Ile Ser Lys Ile Ser Ser Asn Gly Thr Ile Leu Tyr Lys Asn

100 105 110

Asn Ser Ser Asn Ser Ser Asn Ile Ala Leu Gln Pro Lys Asn Asp Gly

115 120 125

Phe Lys Ala Leu Ile Asn Ile Asn Asp Lys Leu Ala Asn Lys Glu Tyr

130 135 140

Glu Phe Thr Phe Asn Leu Pro Lys Asn Ser Lys Leu Ile Ser Ala Ala

145 150 155 160

Thr Tyr Leu Gly Lys Glu Tyr Asp Thr Lys Glu Val Phe Val Val Asp

165 170 175

Lys Asn Asn Ile Ile Thr Ser Ile Ile Ser Pro Ala Trp Ala Lys Asp

180 185 190

Ala Asn Gly His Asn Val Ser Thr Tyr Tyr Lys Ile Val Ser Asn Asn

195 200 205

Lys Leu Val Gln Val Val Glu Phe Thr Glu Asn Thr Ala Phe Pro Val

210 215 220

Val Ala Asp Pro Asn Trp Thr Lys Ile Gly Lys Cys Ala Gly Ser Ile

225 230 235 240

Ala Trp Ala Ile Gly Ser Gly Leu Phe Gly Gly Ala Lys Leu Ile Lys

245 250 255

Ile Lys Lys Tyr Ile Ala Glu Leu Gly Gly Leu Gln Lys Ala Ala Lys

260 265 270

Leu Leu Val Gly Ala Thr Thr Trp Glu Glu Lys Leu His Ala Gly Gly

275 280 285

Tyr Ala Leu Ile Asn Leu Ala Ala Glu Leu Thr Gly Val Ala Gly Ile

290 295 300

Gln Ala Asn Cys Phe

305

<210> 49

<211> 930

<212> DNA

<213> 酪丁酸梭菌(Clostridium tyrobutyricum)

<400> 49

ttgagaaaag tatttttaag atcaataatt tcaacattag ttatgtgtgc atttgtttca 60

agcagctttt cagtaaatgc ggatgaaagc aaaccaaatg atgaaaaaat aattaataac 120

atagaaaacg ttactactac taaagatatt gtaaaaagta ataaaaataa tattgtatat 180

ttagatgaag gtgtaatgag tattccattg tctgggagaa aacccattgc tattaaagat 240

gataataata aagaagattt aactgttaca ttacctatta agaatactgg agatatatct 300

aaaattagta gtaatggtac tattctgtat aaaaataata gtagtaattc atctaatata 360

gctttacaac ctaaaaatga tggatttaag gctttaataa atattaatga taagttagct 420

aataaagaat atgaatttac atttaattta cccaaaaaca gtaaattaat tagtgctgcc 480

acatatttgg gtaaagaata tgatacaaaa gaagtatttg tagtagacaa aaataatata 540

attacgagta ttattagtcc agcttgggct aaagatgcaa atggacataa tgtttctact 600

tattataaga tagtatcgaa taataaatta gtacaagttg ttgaattcac agaaaatact 660

gcattcccgg tggtagctga tcctaattgg actaaaattg ggaaatgcgc tgggtcaata 720

gcatgggcta taggttctgg cctttttggt ggagcaaagc taattaaaat aaaaaaatat 780

atagcagagc ttggaggact tcaaaaagca gctaaattat tagttggtgc aaccacttgg 840

gaagaaaaat tacacgcagg cggttatgca ttaattaact tagctgctga gctaacaggt 900

gtagcaggta tacaagcaaa ttgtttttaa 930

<210> 50

<211> 62

<212> PRT

<213> 凝结芽孢杆菌(Bacillus coagulans)

<400> 50

Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile Ile

1 5 10 15

Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser Cys

20 25 30

Ser Val Asp Trp Gly Lys Ala Thr Thr Cys Ile Ile Asn Asn Gly Ala

35 40 45

Met Ala Trp Ala Thr Gly Gly His Gln Gly Thr His Lys Cys

50 55 60

<210> 51

<211> 189

<212> DNA

<213> 凝结芽孢杆菌(Bacillus coagulans)

<400> 51

atgaaaaaaa ttgaaaaatt aactgaaaaa gaaatggcca atatcattgg tggtaaatac 60

tacggtaatg gggttacttg tggcaaacat tcctgctctg ttgactgggg taaggctacc 120

acctgcataa tcaataatgg agctatggca tgggctactg gtggacatca aggtactcat 180

aaatgctag 189

<210> 52

<211> 490

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 52

Met Asp Lys Val Thr Asp Asn Ser Pro Asp Val Glu Ser Thr Glu Ser

1 5 10 15

Thr Glu Gly Ser Phe Pro Thr Val Gly Val Asp Thr Gly Asp Thr Ile

20 25 30

Thr Ala Thr Leu Ala Thr Gly Thr Glu Asn Val Gly Gly Gly Gly Gly

35 40 45

Ala Phe Gly Gly Ala Ser Glu Ser Ser Ala Ala Ile His Ala Thr Ala

50 55 60

Lys Trp Ser Thr Ala Gln Leu Lys Lys His Gln Ala Glu Gln Ala Ala

65 70 75 80

Arg Ala Ala Ala Ala Glu Ala Ala Leu Ala Lys Ala Lys Ser Gln Arg

85 90 95

Asp Ala Leu Thr Gln Arg Leu Lys Asp Ile Val Asn Asp Ala Leu Arg

100 105 110

Ala Asn Ala Ala Arg Ser Pro Ser Val Thr Asp Leu Ala His Ala Asn

115 120 125

Asn Met Ala Met Gln Ala Glu Ala Glu Arg Leu Arg Leu Ala Lys Ala

130 135 140

Glu Gln Lys Ala Arg Glu Glu Ala Glu Ala Ala Glu Lys Ala Leu Arg

145 150 155 160

Glu Ala Glu Arg Gln Arg Asp Glu Ile Ala Arg Gln Gln Ala Glu Thr

165 170 175

Ala His Leu Leu Ala Met Ala Glu Ala Ala Glu Ala Glu Lys Asn Arg

180 185 190

Gln Asp Ser Leu Asp Glu Glu His Arg Ala Val Glu Val Ala Glu Lys

195 200 205

Lys Leu Ala Glu Ala Lys Ala Glu Leu Ala Lys Ala Glu Ser Asp Val

210 215 220

Gln Ser Lys Gln Ala Ile Val Ser Arg Val Ala Gly Glu Leu Glu Asn

225 230 235 240

Ala Gln Lys Ser Val Asp Val Lys Val Thr Gly Phe Pro Gly Trp Arg

245 250 255

Asp Val Gln Lys Lys Leu Glu Arg Gln Leu Gln Asp Lys Lys Asn Glu

260 265 270

Tyr Ser Ser Val Thr Asn Ala Leu Asn Ser Ala Val Ser Ile Arg Asp

275 280 285

Ala Lys Lys Thr Glu Val Gln Asn Ala Glu Ile Lys Leu Lys Glu Ala

290 295 300

Lys Asp Ala Leu Glu Lys Ser Gln Val Lys Asp Ser Val Asp Thr Met

305 310 315 320

Val Gly Phe Tyr Gln Tyr Ile Thr Glu Gln Tyr Gly Glu Lys Tyr Ser

325 330 335

Arg Ile Ala Gln Asp Leu Ala Glu Lys Ala Lys Gly Ser Lys Phe Asn

340 345 350

Ser Val Asp Glu Ala Leu Ala Ala Phe Glu Lys Tyr Lys Asn Val Leu

355 360 365

Asp Lys Lys Phe Ser Lys Val Asp Arg Asp Asp Ile Phe Asn Ala Leu

370 375 380

Glu Ser Ile Thr Tyr Asp Glu Trp Ala Lys His Leu Glu Lys Ile Ser

385 390 395 400

Arg Ala Leu Lys Val Thr Gly Tyr Leu Ser Phe Gly Tyr Asp Val Trp

405 410 415

Asp Gly Thr Leu Lys Gly Leu Lys Thr Gly Asp Trp Lys Pro Leu Phe

420 425 430

Val Thr Leu Glu Lys Ser Ala Val Asp Phe Gly Val Ala Lys Ile Val

435 440 445

Ala Leu Met Phe Ser Phe Ile Val Gly Ala Pro Leu Gly Phe Trp Gly

450 455 460

Ile Ala Ile Ile Thr Gly Ile Val Ser Ser Tyr Ile Gly Asp Asp Glu

465 470 475 480

Leu Asn Lys Leu Asn Glu Leu Leu Gly Ile

485 490

<210> 53

<211> 1473

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 53

atggataaag tcactgataa ttctccagat gtggagagca cagaatctac tgaggggtca 60

ttcccaactg ttggggttga tactggcgat acgattacag cgacgcttgc aactggaact 120

gaaaatgttg gtggaggcgg tggagcattt ggtggggcca gtgaaagttc tgctgcgata 180

catgcaaccg ctaaatggtc taccgcgcag ttgaaaaaac atcaggctga acaggctgcc 240

cgtgctgctg cggctgaggc agcattggca aaagcgaaat ctcagcgtga tgccctgact 300

caacgtctca aggatattgt taatgacgct ttacgtgcta atgccgctcg tagtccatca 360

gtaactgacc ttgctcatgc caataatatg gcaatgcagg cagaggctga gcgtttgcgc 420

cttgcgaagg cagagcaaaa agcccgtgaa gaagctgaag cagcagaaaa agcgctccgg 480

gaagcagaac gccaacgtga tgagattgcc cgccaacagg ctgaaaccgc gcatttgtta 540

gcaatggcgg aggcagcaga ggctgagaaa aatcgacagg attctcttga tgaagagcat 600

cgggctgtgg aagtggcaga gaagaagctg gctgaggcta aagctgaact ggcgaaggcc 660

gaaagcgatg tacagagtaa gcaagcgatt gtttccagag ttgcagggga gcttgaaaac 720

gctcaaaaaa gtgttgatgt gaaggttacc ggatttcctg gatggcgtga tgttcagaaa 780

aaactggaga gacaattgca ggataagaag aatgaatatt cgtcagtgac gaatgctctt 840

aattctgctg ttagcattag agatgctaaa aaaacagaag ttcagaatgc tgagataaaa 900

ttaaaagaag ctaaggatgc tcttgagaag agtcaggtaa aagactctgt tgatactatg 960

gttgggtttt atcaatatat aaccgaacaa tatggggaaa aatattccag aatagctcag 1020

gatttagctg aaaaggcgaa gggtagtaaa tttaatagtg ttgatgaagc acttgctgca 1080

tttgaaaagt ataaaaatgt actggataag aaattcagta aggttgatag ggatgatatt 1140

tttaatgctt tagagtctat tacttatgat gagtgggcca agcatctaga aaagatctct 1200

agggctctta aggttactgg atatttgtct ttcgggtatg atgtatggga tggtacccta 1260

aagggattaa aaacaggaga ctggaagcct ttatttgtca ctctggagaa gagcgcggta 1320

gatttcggcg tggcaaaaat tgtggcatta atgtttagtt ttattgttgg tgcgcctctt 1380

ggcttctggg gaattgcaat tatcacaggt attgtttctt cttacatagg ggatgatgag 1440

ttgaacaagc ttaatgaatt actaggtatt taa 1473

<210> 54

<211> 522

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 54

Met Glu Thr Ala Val Ala Tyr Tyr Lys Asp Gly Val Pro Tyr Asp Asp

1 5 10 15

Lys Gly Gln Val Ile Ile Thr Leu Leu Asn Gly Thr Pro Asp Gly Ser

20 25 30

Gly Ser Gly Gly Gly Gly Gly Lys Gly Gly Ser Lys Ser Glu Ser Ser

35 40 45

Ala Ala Ile His Ala Thr Ala Lys Trp Ser Thr Ala Gln Leu Lys Lys

50 55 60

Thr Gln Ala Glu Gln Ala Ala Arg Ala Lys Ala Ala Ala Glu Ala Gln

65 70 75 80

Ala Lys Ala Lys Ala Asn Arg Asp Ala Leu Thr Gln Arg Leu Lys Asp

85 90 95

Ile Val Asn Glu Ala Leu Arg His Asn Ala Ser Arg Thr Pro Ser Ala

100 105 110

Thr Glu Leu Ala His Ala Asn Asn Ala Ala Met Gln Ala Glu Asp Glu

115 120 125

Arg Leu Arg Leu Ala Lys Ala Glu Glu Lys Ala Arg Lys Glu Ala Glu

130 135 140

Ala Ala Glu Lys Ala Phe Gln Glu Ala Glu Gln Arg Arg Lys Glu Ile

145 150 155 160

Glu Arg Glu Lys Ala Glu Thr Glu Arg Gln Leu Lys Leu Ala Glu Ala

165 170 175

Glu Glu Lys Arg Leu Ala Ala Leu Ser Glu Glu Ala Lys Ala Val Glu

180 185 190

Ile Ala Gln Lys Lys Leu Ser Ala Ala Gln Ser Glu Val Val Lys Met

195 200 205

Asp Gly Glu Ile Lys Thr Leu Asn Ser Arg Leu Ser Ser Ser Ile His

210 215 220

Ala Arg Asp Ala Glu Met Lys Thr Leu Ala Gly Lys Arg Asn Glu Leu

225 230 235 240

Ala Gln Ala Ser Ala Lys Tyr Lys Glu Leu Asp Glu Leu Val Lys Lys

245 250 255

Leu Ser Pro Arg Ala Asn Asp Pro Leu Gln Asn Arg Pro Phe Phe Glu

260 265 270

Ala Thr Arg Arg Arg Val Gly Ala Gly Lys Ile Arg Glu Glu Lys Gln

275 280 285

Lys Gln Val Thr Ala Ser Glu Thr Arg Ile Asn Arg Ile Asn Ala Asp

290 295 300

Ile Thr Gln Ile Gln Lys Ala Ile Ser Gln Val Ser Asn Asn Arg Asn

305 310 315 320

Ala Gly Ile Ala Arg Val His Glu Ala Glu Glu Asn Leu Lys Lys Ala

325 330 335

Gln Asn Asn Leu Leu Asn Ser Gln Ile Lys Asp Ala Val Asp Ala Thr

340 345 350

Val Ser Phe Tyr Gln Thr Leu Thr Glu Lys Tyr Gly Glu Lys Tyr Ser

355 360 365

Lys Met Ala Gln Glu Leu Ala Asp Lys Ser Lys Gly Lys Lys Ile Gly

370 375 380

Asn Val Asn Glu Ala Leu Ala Ala Phe Glu Lys Tyr Lys Asp Val Leu

385 390 395 400

Asn Lys Lys Phe Ser Lys Ala Asp Arg Asp Ala Ile Phe Asn Ala Leu

405 410 415

Ala Ser Val Lys Tyr Asp Asp Trp Ala Lys His Leu Asp Gln Phe Ala

420 425 430

Lys Tyr Leu Lys Ile Thr Gly His Val Ser Phe Gly Tyr Asp Val Val

435 440 445

Ser Asp Ile Leu Lys Ile Lys Asp Thr Gly Asp Trp Lys Pro Leu Phe

450 455 460

Leu Thr Leu Glu Lys Lys Ala Ala Asp Ala Gly Val Ser Tyr Val Val

465 470 475 480

Ala Leu Leu Phe Ser Leu Leu Ala Gly Thr Thr Leu Gly Ile Trp Gly

485 490 495

Ile Ala Ile Val Thr Gly Ile Leu Cys Ser Tyr Ile Asp Lys Asn Lys

500 505 510

Leu Asn Thr Ile Asn Glu Val Leu Gly Ile

515 520

<210> 55

<211> 1569

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 55

atggaaaccg cggtagcgta ctataaagat ggtgttcctt atgatgataa gggacaggta 60

attattactc ttttgaatgg tactcctgac gggagtggct ctggcggcgg aggtggaaaa 120

ggaggcagta aaagtgaaag ttctgcagct attcatgcaa ctgctaaatg gtctactgct 180

caattaaaga aaacacaggc agagcaggct gcccgggcaa aagctgcagc ggaagcacag 240

gcgaaagcaa aggcaaacag ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag 300

gctcttcgtc acaatgcctc acgtacgcct tcagcaacag agcttgctca tgctaataat 360

gcagctatgc aggcggaaga cgagcgtttg cgccttgcga aagcagaaga aaaagcccgt 420

aaagaagcgg aagcagcaga aaaggctttt caggaagcag aacaacgacg taaagagatt 480

gaacgggaga aggctgaaac agaacgccag ttgaaactgg ctgaagctga agagaaacga 540

ctggctgcat tgagtgaaga agctaaagct gttgagatcg cccaaaaaaa actttctgct 600

gcacaatctg aagtggtgaa aatggatgga gagattaaga ctctcaattc tcgtttaagc 660

tccagtatcc atgcccgtga tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg 720

gctcaggcat ccgctaaata taaagaactg gatgagctgg tcaaaaaact atcaccaaga 780

gccaatgatc cgcttcagaa ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc 840

ggtaagatta gagaagaaaa acaaaaacag gtaacagcat cagaaacacg tattaaccgg 900

ataaatgctg atataactca gatccagaag gctatttctc aggtcagtaa taatcgtaat 960

gccggtatcg ctcgtgttca tgaagctgaa gaaaatttga aaaaagcaca gaataatctc 1020

cttaattcac agattaagga tgctgttgat gcaacagtta gcttttatca aacgctgact 1080

gaaaaatatg gtgaaaaata ttcgaaaatg gcacaggaac ttgctgataa gtctaaaggt 1140

aagaaaatcg gcaatgtgaa tgaagctctc gctgcttttg aaaaatacaa ggatgtttta 1200

aataagaaat tcagcaaagc cgatcgtgat gctattttta atgcgttggc atcggtgaag 1260

tatgatgact gggctaaaca tttagatcag tttgccaagt acttgaagat tacggggcat 1320

gtttcttttg gatatgatgt ggtatctgat atcctaaaaa ttaaggatac aggtgactgg 1380

aagccactat ttcttacatt agagaagaaa gctgcagatg caggggtgag ttatgttgtt 1440

gctttacttt ttagcttgct tgctggaact acattaggta tttggggtat tgctattgtt 1500

acaggaattc tatgctccta tattgataag aataaactta atactataaa tgaggtgtta 1560

gggatttaa 1569

<210> 56

<211> 626

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 56

Met Ser Asp Pro Val Arg Ile Thr Asn Pro Gly Ala Glu Ser Leu Gly

1 5 10 15

Tyr Asp Ser Asp Gly His Glu Ile Met Ala Val Asp Ile Tyr Val Asn

20 25 30

Pro Pro Arg Val Asp Val Phe His Gly Thr Pro Pro Ala Trp Ser Ser

35 40 45

Phe Gly Asn Lys Thr Ile Trp Gly Gly Asn Glu Trp Val Asp Asp Ser

50 55 60

Pro Thr Arg Ser Asp Ile Glu Lys Arg Asp Lys Glu Ile Thr Ala Tyr

65 70 75 80

Lys Asn Thr Leu Ser Ala Gln Gln Lys Glu Asn Glu Asn Lys Arg Thr

85 90 95

Glu Ala Gly Lys Arg Leu Ser Ala Ala Ile Ala Ala Arg Glu Lys Asp

100 105 110

Glu Asn Thr Leu Lys Thr Leu Arg Ala Gly Asn Ala Asp Ala Ala Asp

115 120 125

Ile Thr Arg Gln Glu Phe Arg Leu Leu Gln Ala Glu Leu Arg Glu Tyr

130 135 140

Gly Phe Arg Thr Glu Ile Ala Gly Tyr Asp Ala Leu Arg Leu His Thr

145 150 155 160

Glu Ser Arg Met Leu Phe Ala Asp Ala Asp Ser Leu Arg Ile Ser Pro

165 170 175

Arg Glu Ala Arg Ser Leu Ile Glu Gln Ala Glu Lys Arg Gln Lys Asp

180 185 190

Ala Gln Asn Ala Asp Lys Lys Ala Ala Asp Met Leu Ala Glu Tyr Glu

195 200 205

Arg Arg Lys Gly Ile Leu Asp Thr Arg Leu Ser Glu Leu Glu Lys Asn

210 215 220

Gly Gly Ala Ala Leu Ala Val Leu Asp Ala Gln Gln Ala Arg Leu Leu

225 230 235 240

Gly Gln Gln Thr Arg Asn Asp Arg Ala Ile Ser Glu Ala Arg Asn Lys

245 250 255

Leu Ser Ser Val Thr Glu Ser Leu Asn Thr Ala Arg Asn Ala Leu Thr

260 265 270

Arg Ala Glu Gln Gln Leu Thr Gln Gln Lys Asn Thr Pro Asp Gly Lys

275 280 285

Thr Ile Val Ser Pro Glu Lys Phe Pro Gly Arg Ser Ser Thr Asn His

290 295 300

Ser Ile Val Val Ser Gly Asp Pro Arg Phe Ala Gly Thr Ile Lys Ile

305 310 315 320

Thr Thr Ser Ala Val Ile Asp Asn Arg Ala Asn Leu Asn Tyr Leu Leu

325 330 335

Ser His Ser Gly Leu Asp Tyr Lys Arg Asn Ile Leu Asn Asp Arg Asn

340 345 350

Pro Val Val Thr Glu Asp Val Glu Gly Asp Lys Lys Ile Tyr Asn Ala

355 360 365

Glu Val Ala Glu Trp Asp Lys Leu Arg Gln Arg Leu Leu Asp Ala Arg

370 375 380

Asn Lys Ile Thr Ser Ala Glu Ser Ala Val Asn Ser Ala Arg Asn Asn

385 390 395 400

Leu Ser Ala Arg Thr Asn Glu Gln Lys His Ala Asn Asp Ala Leu Asn

405 410 415

Ala Leu Leu Lys Glu Lys Glu Asn Ile Arg Asn Gln Leu Ser Gly Ile

420 425 430

Asn Gln Lys Ile Ala Glu Glu Lys Arg Lys Gln Asp Glu Leu Lys Ala

435 440 445

Thr Lys Asp Ala Ile Asn Phe Thr Thr Glu Phe Leu Lys Ser Val Ser

450 455 460

Glu Lys Tyr Gly Ala Lys Ala Glu Gln Leu Ala Arg Glu Met Ala Gly

465 470 475 480

Gln Ala Lys Gly Lys Lys Ile Arg Asn Val Glu Glu Ala Leu Lys Thr

485 490 495

Tyr Glu Lys Tyr Arg Ala Asp Ile Asn Lys Lys Ile Asn Ala Lys Asp

500 505 510

Arg Ala Ala Ile Ala Ala Ala Leu Glu Ser Val Lys Leu Ser Asp Ile

515 520 525

Ser Ser Asn Leu Asn Arg Phe Ser Arg Gly Leu Gly Tyr Ala Gly Lys

530 535 540

Phe Thr Ser Leu Ala Asp Trp Ile Thr Glu Phe Gly Lys Ala Val Arg

545 550 555 560

Thr Glu Asn Trp Arg Pro Leu Phe Val Lys Thr Glu Thr Ile Ile Ala

565 570 575

Gly Asn Ala Ala Thr Ala Leu Val Ala Leu Val Phe Ser Ile Leu Thr

580 585 590

Gly Ser Ala Leu Gly Ile Ile Gly Tyr Gly Leu Leu Met Ala Val Thr

595 600 605

Gly Ala Leu Ile Asp Glu Ser Leu Val Glu Lys Ala Asn Lys Phe Trp

610 615 620

Gly Ile

625

<210> 57

<211> 1881

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 57

atgtctgacc ctgtacgtat tacaaatccc ggtgcagaat cgctggggta tgattcagat 60

ggccatgaaa ttatggccgt tgatatttat gtaaaccctc cacgtgtcga tgtctttcat 120

ggtaccccgc ctgcatggag ttccttcggg aacaaaacca tctggggcgg aaacgagtgg 180

gttgatgatt ccccaacccg aagtgatatc gaaaaaaggg acaaggaaat cacagcgtac 240

aaaaacacgc tcagcgcgca gcagaaagag aatgagaata agcgtactga agccggaaaa 300

cgcctctctg cggcgattgc tgcaagggaa aaagatgaaa acacactgaa aacactccgt 360

gccggaaacg cagatgccgc tgatattaca cgacaggagt tcagactcct gcaggcagag 420

ctgagagaat acggattccg tactgaaatc gccggatatg acgccctccg gctgcataca 480

gagagccgga tgctgtttgc tgatgctgat tctcttcgta tatctccccg ggaggccagg 540

tcgttaatcg aacaggctga aaaacggcag aaggatgcgc agaacgcaga caagaaggcc 600

gctgatatgc ttgctgaata cgagcgcaga aaaggtattc tggacacccg gttgtcagag 660

ctggaaaaaa atggcggggc agcccttgcc gttcttgatg cacaacaggc ccgtctgctc 720

gggcagcaga cacggaatga cagggccatt tcagaggccc ggaataaact cagttcagtg 780

acggaatcgc ttaacacggc ccgtaatgca ttaaccagag ctgaacaaca gctgacgcaa 840

cagaaaaaca cgcctgacgg caaaacgata gtttcccctg aaaaattccc ggggcgttca 900

tcaacaaatg attctattgt tgtgagcggt gatccgagat ttgccggtac gataaaaatc 960

acaaccagcg cagtcatcga taaccgtgca aacctgaatt atcttctgag ccattccggt 1020

ctggactata aacgcaatat tctgaatgac cggaatccgg tggtgacaga ggatgtggaa 1080

ggtgacaaga aaatttataa tgctgaagtt gctgaatggg ataagttacg gcaaagattg 1140

cttgatgcca gaaataaaat cacctctgct gaatctgcgg taaattcggc gagaaataac 1200

ctcagtgcca gaacaaatga gcaaaagcat gcaaatgacg ctcttaatgc cctgttgaag 1260

gaaaaagaga atatccgtaa ccagctttcc ggcatcaatc agaagatagc ggaagagaaa 1320

agaaaacagg atgaactgaa ggcaacgaaa gacgcaatta atttcacaac agagttcctg 1380

aaatcagttt cagaaaaata tggtgcaaaa gctgagcagt tagccagaga gatggccggg 1440

caggctaaag ggaagaaaat acgtaatgtt gaagaggcat taaaaacgta tgaaaagtac 1500

cgggctgaca ttaacaaaaa aattaatgca aaagatcgtg cagcgattgc cgcagccctt 1560

gagtctgtga agctgtctga tatatcgtct aatctgaaca gattcagtcg gggactggga 1620

tatgcaggaa aatttacaag tcttgctgac tggatcactg agtttggtaa ggctgtccgg 1680

acagagaact ggcgtcctct ttttgttaaa acagaaacca tcatagcagg caatgccgca 1740

acggctcttg tggcactggt cttcagtatt cttaccggaa gcgctttagg cattatcggg 1800

tatggtttac tgatggctgt caccggtgcg ctgattgatg aatcgcttgt ggaaaaagcg 1860

aataagttct ggggtattta a 1881

<210> 58

<211> 626

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 58

Met Ser Asp Pro Val Arg Ile Thr Asn Pro Gly Ala Glu Ser Leu Gly

1 5 10 15

Tyr Asp Ser Asp Gly His Glu Ile Met Ala Val Asp Ile Tyr Val Asn

20 25 30

Pro Pro Arg Val Asp Val Phe His Gly Thr Pro Pro Ala Trp Ser Ser

35 40 45

Phe Gly Asn Lys Thr Ile Trp Gly Gly Asn Glu Trp Val Asp Asp Ser

50 55 60

Pro Thr Arg Ser Asp Ile Glu Lys Arg Asp Lys Glu Ile Thr Ala Tyr

65 70 75 80

Lys Asn Thr Leu Ser Ala Gln Gln Lys Glu Asn Glu Asn Lys Arg Thr

85 90 95

Glu Ala Gly Lys Arg Leu Ser Ala Ala Ile Ala Ala Arg Glu Lys Asp

100 105 110

Glu Asn Thr Leu Lys Thr Leu Arg Ala Gly Asn Ala Asp Ala Ala Asp

115 120 125

Ile Thr Arg Gln Glu Phe Arg Leu Leu Gln Ala Glu Leu Arg Glu Tyr

130 135 140

Gly Phe Arg Thr Glu Ile Ala Gly Tyr Asp Ala Leu Arg Leu His Thr

145 150 155 160

Glu Ser Arg Met Leu Phe Ala Asp Ala Asp Ser Leu Arg Ile Ser Pro

165 170 175

Arg Glu Ala Arg Ser Leu Ile Glu Gln Ala Glu Lys Arg Gln Lys Asp

180 185 190

Ala Gln Asn Ala Asp Lys Lys Ala Ala Asp Met Leu Ala Glu Tyr Glu

195 200 205

Arg Arg Lys Gly Ile Leu Asp Thr Arg Leu Ser Glu Leu Glu Lys Asn

210 215 220

Gly Gly Ala Ala Leu Ala Val Leu Asp Ala Gln Gln Ala Arg Leu Leu

225 230 235 240

Gly Gln Gln Thr Arg Asn Asp Arg Ala Ile Ser Glu Ala Arg Asn Lys

245 250 255

Leu Ser Ser Val Thr Glu Ser Leu Lys Thr Ala Arg Asn Ala Leu Thr

260 265 270

Arg Ala Glu Gln Gln Leu Thr Gln Gln Lys Asn Thr Pro Asp Gly Lys

275 280 285

Thr Ile Val Ser Pro Glu Lys Phe Pro Gly Arg Ser Ser Thr Asn His

290 295 300

Ser Ile Val Val Ser Gly Asp Pro Arg Phe Ala Gly Thr Ile Lys Ile

305 310 315 320

Thr Thr Ser Ala Val Ile Asp Asn Arg Ala Asn Leu Asn Tyr Leu Leu

325 330 335

Thr His Ser Gly Leu Asp Tyr Lys Arg Asn Ile Leu Asn Asp Arg Asn

340 345 350

Pro Val Val Thr Glu Asp Val Glu Gly Asp Lys Lys Ile Tyr Asn Ala

355 360 365

Glu Val Ala Glu Trp Asp Lys Leu Arg Gln Arg Leu Leu Asp Ala Arg

370 375 380

Asn Lys Ile Thr Ser Ala Glu Ser Ala Ile Asn Ser Ala Arg Asn Asn

385 390 395 400

Val Ser Ala Arg Thr Asn Glu Gln Lys His Ala Asn Asp Ala Leu Asn

405 410 415

Ala Leu Leu Lys Glu Lys Glu Asn Ile Arg Ser Gln Leu Ala Asp Ile

420 425 430

Asn Gln Lys Ile Ala Glu Glu Lys Arg Lys Arg Asp Glu Ile Asn Met

435 440 445

Val Lys Asp Ala Ile Lys Leu Thr Ser Asp Phe Tyr Arg Thr Ile Tyr

450 455 460

Asp Glu Phe Gly Lys Gln Ala Ser Glu Leu Ala Lys Glu Leu Ala Ser

465 470 475 480

Val Ser Gln Gly Lys Gln Ile Lys Ser Val Asp Asp Ala Leu Asn Ala

485 490 495

Phe Asp Lys Phe Arg Asn Asn Leu Asn Lys Lys Tyr Asn Ile Gln Asp

500 505 510

Arg Met Ala Ile Ser Lys Ala Leu Glu Ala Ile Asn Gln Val His Met

515 520 525

Ala Glu Asn Phe Lys Leu Phe Ser Lys Ala Phe Gly Phe Thr Gly Lys

530 535 540

Val Ile Glu Arg Tyr Asp Val Ala Val Glu Leu Gln Lys Ala Val Lys

545 550 555 560

Thr Asp Asn Trp Arg Pro Phe Phe Val Lys Leu Glu Ser Leu Ala Ala

565 570 575

Gly Arg Ala Ala Ser Ala Val Thr Ala Trp Ala Phe Ser Val Met Leu

580 585 590

Gly Thr Pro Val Gly Ile Leu Gly Phe Ala Ile Ile Met Ala Ala Val

595 600 605

Ser Ala Leu Val Asn Asp Lys Phe Ile Glu Gln Val Asn Lys Leu Ile

610 615 620

Gly Ile

625

<210> 59

<211> 1881

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 59

atgtctgacc ctgtacgtat tacaaatccc ggtgcagaat cgctgggata tgattcagat 60

ggccatgaaa ttatggccgt tgatatttat gtaaaccctc cacgtgtcga tgtctttcat 120

ggtaccccgc ctgcatggag ttccttcggg aacaaaacca tctggggtgg aaacgagtgg 180

gtcgatgatt ccccaacccg aagtgatatc gaaaaaaggg acaaggaaat cacagcgtac 240

aaaaacacgc tcagcgcgca gcagaaagag aatgagaata agcgtactga agctggaaaa 300

cgcctttctg cggcaattgc tgcaagggaa aaagatgaaa acacactgaa aacactccgt 360

gccggaaacg cagatgccgc tgatattaca cgacaggagt tcagactcct gcaggcagag 420

ctgagagaat acggattccg tactgaaatc gccggatatg atgccctccg gctgcataca 480

gagagccgga tgctgtttgc tgatgctgat tctcttcgta tatctccccg cgaggccagg 540

tcgttaatcg aacaggctga aaaacggcag aaggatgcgc agaacgcaga caagaaggcc 600

gctgatatgc ttgctgaata cgagcgcaga aaaggtattc tggacacgcg gttgtcagag 660

ctggaaaaaa atggcggggc agcccttgcc gttcttgatg cacaacaggc ccgtctgctc 720

gggcagcaga cacggaatga cagggccatt tcagaggccc ggaataaact cagttcggtg 780

acggaatcgc ttaagacggc ccgtaatgca ttaaccagag ctgaacaaca gctgacgcaa 840

cagaaaaaca cgcctgacgg caaaacgata gtttcccctg aaaaattccc ggggcgttca 900

tcaacaaatc attctattgt tgtgagtggt gatccgaggt ttgccggtac gataaaaatc 960

acaaccagcg cggtcatcga taaccgtgca aacctgaatt atcttctgac ccattccggt 1020

ctggactata aacgcaatat tctgaatgac cggaatccgg tggtgacaga ggatgtggaa 1080

ggtgacaaga aaatttataa tgctgaagtt gctgaatggg ataagttacg gcaacgattg 1140

cttgatgcca gaaataaaat cacctctgct gaatctgcga taaattcggc gagaaataac 1200

gtcagtgcca gaacaaatga acaaaagcat gcaaatgacg ctcttaatgc cctgttgaag 1260

gaaaaagaga atatccgtag ccagcttgct gacatcaatc agaaaatagc tgaagagaaa 1320

agaaaaaggg atgaaataaa tatggtaaag gatgccataa aactcacctc tgatttctac 1380

agaacgatat atgatgagtt cggtaaacaa gcatccgaac ttgctaagga gctggcttct 1440

gtatctcaag ggaaacagat taagagtgtg gatgatgcac tgaacgcttt tgataaattc 1500

cgtaataatc tgaacaagaa atataacata caagatcgca tggccatttc taaagccctg 1560

gaagctatta atcaggtcca tatggcggag aattttaagc tgttcagtaa ggcatttggt 1620

tttaccggaa aagttattga acgttatgat gttgctgtgg agttacaaaa ggctgtaaaa 1680

acggacaact ggcgtccatt ttttgtaaaa cttgaatcac tggcagcagg aagagctgct 1740

tcagcagtta cagcatgggc gttttccgtc atgctgggaa cccctgtagg tattctgggt 1800

tttgcaatta ttatggcggc tgtgagtgcg cttgttaatg ataagtttat tgagcaggtc 1860

aataaactta ttggtatctg a 1881

<210> 60

<211> 271

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 60

Met Glu Thr Leu Thr Val His Ala Pro Ser Pro Ser Thr Asn Leu Pro

1 5 10 15

Ser Tyr Gly Asn Gly Ala Phe Ser Leu Ser Ala Pro His Val Pro Gly

20 25 30

Ala Gly Pro Leu Leu Val Gln Val Val Tyr Ser Phe Phe Gln Ser Pro

35 40 45

Asn Met Cys Leu Gln Ala Leu Thr Gln Leu Glu Asp Tyr Ile Lys Lys

50 55 60

His Gly Ala Ser Asn Pro Leu Thr Leu Gln Ile Ile Ser Thr Asn Ile

65 70 75 80

Gly Tyr Phe Cys Asn Ala Asp Arg Asn Leu Val Leu His Pro Gly Ile

85 90 95

Ser Val Tyr Asp Ala Tyr His Phe Ala Lys Pro Ala Pro Ser Gln Tyr

100 105 110

Asp Tyr Arg Ser Met Asn Met Lys Gln Met Ser Gly Asn Val Thr Thr

115 120 125

Pro Ile Val Ala Leu Ala His Tyr Leu Trp Gly Asn Gly Ala Glu Arg

130 135 140

Ser Val Asn Ile Ala Asn Ile Gly Leu Lys Ile Ser Pro Met Lys Ile

145 150 155 160

Asn Gln Ile Lys Asp Ile Ile Lys Ser Gly Val Val Gly Thr Phe Pro

165 170 175

Val Ser Thr Lys Phe Thr His Ala Thr Gly Asp Tyr Asn Val Ile Thr

180 185 190

Gly Ala Tyr Leu Gly Asn Ile Thr Leu Lys Thr Glu Gly Thr Leu Thr

195 200 205

Ile Ser Ala Asn Gly Ser Trp Thr Tyr Asn Gly Val Val Arg Ser Tyr

210 215 220

Asp Asp Lys Tyr Asp Phe Asn Ala Ser Thr His Arg Gly Ile Ile Gly

225 230 235 240

Glu Ser Leu Thr Arg Leu Gly Ala Met Phe Ser Gly Lys Glu Tyr Gln

245 250 255

Ile Leu Leu Pro Gly Glu Ile His Ile Lys Glu Ser Gly Lys Arg

260 265 270

<210> 61

<211> 816

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 61

atggaaacct taactgttca tgcaccatca ccatcaacta acttaccaag ttatggcaat 60

ggtgcatttt ctctttcagc accacatgtg cctggtgctg gccctctttt agtccaggtt 120

gtttatagtt ttttccagag tccaaacatg tgtcttcagg ctttaactca acttgaggat 180

tacatcaaaa aacatggggc cagcaaccct ctcacattgc agatcatatc gacaaatatt 240

ggttacttct gtaacgccga ccgaaatctg gttcttcacc ctggaataag cgtttatgac 300

gcttaccact tcgcaaaacc agcgccaagt caatatgact atcgctcaat gaatatgaaa 360

caaatgagcg gtaatgtcac tacaccaatt gtggcgcttg ctcactattt atggggtaat 420

ggcgctgaaa ggagcgttaa tatcgccaac attggtctta aaatttcccc tatgaaaatt 480

aatcagataa aagacattat aaaatctggt gtagtaggca cattccctgt ttctacaaag 540

ttcacacatg ccactggtga ttataatgtt attaccggtg catatcttgg taatatcaca 600

ctgaaaacag aaggtacttt aactatctct gccaatggct cctggactta caatggcgtt 660

gttcgttcat atgatgataa atacgatttt aacgccagca ctcaccgtgg cattatcgga 720

gagtcgctca caaggctcgg ggcgatgttt tctggtaaag agtaccagat actgcttcct 780

ggtgaaattc acattaaaga aagtggtaag cgataa 816

<210> 62

<211> 387

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 62

Met Gly Ser Asn Gly Ala Asp Asn Ala His Asn Asn Ala Phe Gly Gly

1 5 10 15

Gly Lys Asn Pro Gly Ile Gly Asn Thr Ser Gly Ala Gly Ser Asn Gly

20 25 30

Ser Ala Ser Ser Asn Arg Gly Asn Ser Asn Gly Trp Ser Trp Ser Asn

35 40 45

Lys Pro His Lys Asn Asp Gly Phe His Ser Asp Gly Ser Tyr His Ile

50 55 60

Thr Phe His Gly Asp Asn Asn Ser Lys Pro Lys Pro Gly Gly Asn Ser

65 70 75 80

Gly Asn Arg Gly Asn Asn Gly Asp Gly Ala Ser Ala Lys Val Gly Glu

85 90 95

Ile Thr Ile Thr Pro Asp Asn Ser Lys Pro Gly Arg Tyr Ile Ser Ser

100 105 110

Asn Pro Glu Tyr Ser Leu Leu Ala Lys Leu Ile Asp Ala Glu Ser Ile

115 120 125

Lys Gly Thr Glu Val Tyr Thr Phe His Thr Arg Lys Gly Gln Tyr Val

130 135 140

Lys Val Thr Val Pro Asp Ser Asn Ile Asp Lys Met Arg Val Asp Tyr

145 150 155 160

Val Asn Trp Lys Gly Pro Lys Tyr Asn Asn Lys Leu Val Lys Arg Phe

165 170 175

Val Ser Gln Phe Leu Leu Phe Arg Lys Glu Glu Lys Glu Lys Asn Glu

180 185 190

Lys Glu Ala Leu Leu Lys Ala Ser Glu Leu Val Ser Gly Met Gly Asp

195 200 205

Lys Leu Gly Glu Tyr Leu Gly Val Lys Tyr Lys Asn Val Ala Lys Glu

210 215 220

Val Ala Asn Asp Ile Lys Asn Phe His Gly Arg Asn Ile Arg Ser Tyr

225 230 235 240

Asn Glu Ala Met Ala Ser Leu Asn Lys Val Leu Ala Asn Pro Lys Met

245 250 255

Lys Val Asn Lys Ser Asp Lys Asp Ala Ile Val Asn Ala Trp Lys Gln

260 265 270

Val Asn Ala Lys Asp Met Ala Asn Lys Ile Gly Asn Leu Gly Lys Ala

275 280 285

Phe Lys Val Ala Asp Leu Ala Ile Lys Val Glu Lys Ile Arg Glu Lys

290 295 300

Ser Ile Glu Gly Tyr Asn Thr Gly Asn Trp Gly Pro Leu Leu Leu Glu

305 310 315 320

Val Glu Ser Trp Ile Ile Gly Gly Val Val Ala Gly Val Ala Ile Ser

325 330 335

Leu Phe Gly Ala Val Leu Ser Phe Leu Pro Ile Ser Gly Leu Ala Val

340 345 350

Thr Ala Leu Gly Val Ile Gly Ile Met Thr Ile Ser Tyr Leu Ser Ser

355 360 365

Phe Ile Asp Ala Asn Arg Val Ser Asn Ile Asn Asn Ile Ile Ser Ser

370 375 380

Val Ile Arg

385

<210> 63

<211> 51

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 63

gcaaatcgag tttcgaatat aaataacatt atatctagtg ttattcgatg a 51

<210> 64

<211> 103

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 64

Met Arg Thr Leu Thr Leu Asn Glu Leu Asp Ser Val Ser Gly Gly Ala

1 5 10 15

Ser Gly Arg Asp Ile Ala Met Ala Ile Gly Thr Leu Ser Gly Gln Phe

20 25 30

Val Ala Gly Gly Ile Gly Ala Ala Ala Gly Gly Val Ala Gly Gly Ala

35 40 45

Ile Tyr Asp Tyr Ala Ser Thr His Lys Pro Asn Pro Ala Met Ser Pro

50 55 60

Ser Gly Leu Gly Gly Thr Ile Lys Gln Lys Pro Glu Gly Ile Pro Ser

65 70 75 80

Glu Ala Trp Asn Tyr Ala Ala Gly Arg Leu Cys Asn Trp Ser Pro Asn

85 90 95

Asn Leu Ser Asp Val Cys Leu

100

<210> 65

<211> 312

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 65

atgagaactc tgactctaaa tgaattagat tctgtttctg gtggtgcttc agggcgtgat 60

attgcgatgg ctataggaac actatccgga caatttgttg caggaggaat tggagcagct 120

gctgggggtg tggctggagg tgcaatatat gactatgcat ccactcacaa acctaatcct 180

gcaatgtctc catccggttt aggaggaaca attaagcaaa aacccgaagg gataccttca 240

gaagcatgga actatgctgc gggaagattg tgtaattgga gtccaaataa tcttagtgat 300

gtttgtttat aa 312

<210> 66

<211> 58

<212> PRT

<213> 哥伦比亚肠球菌(Enterococcus columbae)

<400> 66

Met Met Asn Ala Thr Glu Asn Gln Ile Phe Val Glu Thr Val Ser Asp

1 5 10 15

Gln Glu Leu Glu Met Leu Ile Gly Gly Ala Gly Arg Gly Trp Ile Lys

20 25 30

Thr Leu Thr Lys Asp Cys Pro Asn Val Ile Ser Ser Ile Cys Ala Gly

35 40 45

Thr Ile Ile Thr Ala Cys Lys Asn Cys Ala

50 55

<210> 67

<211> 177

<212> DNA

<213> 哥伦比亚肠球菌(Enterococcus columbae)

<400> 67

atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa 60

atgttaattg gtggtgcagg tcgtggatgg attaagactt taacaaaaga ttgtccaaat 120

gtgatttctt caatttgtgc aggtacaatt attacagctt gtaaaaattg tgcttaa 177

<210> 68

<211> 59

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 68

Met Asn Asn Val Lys Glu Leu Ser Met Thr Glu Leu Gln Thr Ile Thr

1 5 10 15

Gly Gly Ala Arg Ser Tyr Gly Asn Gly Val Tyr Cys Asn Asn Lys Lys

20 25 30

Cys Trp Val Asn Arg Gly Glu Ala Thr Gln Ser Ile Ile Gly Gly Met

35 40 45

Ile Ser Gly Trp Ala Ser Gly Leu Ala Gly Met

50 55

<210> 69

<211> 180

<212> DNA

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 69

atgaataatg taaaagaatt aagtatgaca gaattacaaa caattaccgg cggtgctaga 60

tcatatggca acggtgttta ctgtaataat aaaaaatgtt gggtaaatcg gggtgaagca 120

acgcaaagta ttattggtgg tatgattagc ggctgggcta gtggtttagc tggaatgtaa 180

<210> 70

<211> 64

<212> PRT

<213> 链霉菌(Streptomyces sp.)

<400> 70

Met Arg Ser Glu Met Thr Leu Thr Ser Thr Asn Ser Ala Glu Ala Leu

1 5 10 15

Ala Ala Gln Asp Phe Ala Asn Thr Val Leu Ser Ala Ala Ala Pro Gly

20 25 30

Phe His Ala Asp Cys Glu Thr Pro Ala Met Ala Thr Pro Ala Thr Pro

35 40 45

Thr Val Ala Gln Phe Val Ile Gln Gly Ser Thr Ile Cys Leu Val Cys

50 55 60

<210> 71

<211> 195

<212> DNA

<213> 链霉菌(Streptomyces sp.)

<400> 71

gtgcgatctg agatgactct tacgagcacg aattccgctg aggctctggc ggcgcaggac 60

tttgcgaaca ccgttctcag cgcggcggcc ccgggcttcc acgcggactg cgagacgccg 120

gccatggcca ccccggccac gccgaccgtc gcccagttcg tgatccaggg cagcacgatc 180

tgcctggtct gctga 195

<210> 72

<211> 65

<212> PRT

<213> 嗜碱芽孢杆菌(Bacillus halodurans)

<400> 72

Met Val Asn Ser Lys Asp Leu Arg Asn Pro Glu Phe Arg Lys Ala Gln

1 5 10 15

Gly Leu Gln Phe Val Asp Glu Val Asn Glu Lys Glu Leu Ser Ser Leu

20 25 30

Ala Gly Ser Gly Asp Val His Ala Gln Thr Thr Trp Pro Cys Ala Thr

35 40 45

Val Gly Val Ser Val Ala Leu Cys Pro Thr Thr Lys Cys Thr Ser Gln

50 55 60

Cys

65

<210> 73

<211> 198

<212> DNA

<213> 嗜碱芽孢杆菌(Bacillus halodurans)

<400> 73

atggtaaatt caaaagattt gcgtaatcct gaattccgca aagcccaagg tctacaattc 60

gttgacgagg tgaacgagaa ggaactttcg tctctagctg gttcaggaga tgtgcatgca 120

caaacaactt ggccttgcgc tacagttggt gtctccgtag ccttgtgccc aactacaaag 180

tgtacaagcc agtgctaa 198

<210> 74

<211> 66

<212> PRT

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 74

Met Lys Asn Leu Lys Glu Gly Ser Tyr Thr Ala Val Asn Thr Asp Glu

1 5 10 15

Leu Lys Ser Ile Asn Gly Gly Thr Lys Tyr Tyr Gly Asn Gly Val Tyr

20 25 30

Cys Asn Ser Lys Lys Cys Trp Val Asp Trp Gly Gln Ala Ser Gly Cys

35 40 45

Ile Gly Gln Thr Val Val Gly Gly Trp Leu Gly Gly Ala Ile Pro Gly

50 55 60

Lys Cys

65

<210> 75

<211> 201

<212> DNA

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 75

atgaaaaact taaaagaagg ttcatacact gctgttaata ctgatgaatt aaaaagtatc 60

aatggtggaa caaaatatta tgggaatggc gtttattgca attctaaaaa atgttgggta 120

gattggggac aagcttcagg ttgtatcggt caaactgttg ttggcggatg gctaggcgga 180

gctataccag gtaaatgcta a 201

<210> 76

<211> 63

<212> PRT

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 76

Met Ile Lys Arg Glu Lys Asn Arg Thr Ile Ser Ser Leu Gly Tyr Glu

1 5 10 15

Glu Ile Ser Asn His Lys Leu Gln Glu Ile Gln Gly Gly Lys Gly Ile

20 25 30

Leu Gly Lys Leu Gly Val Val Gln Ala Gly Val Asp Phe Val Ser Gly

35 40 45

Val Trp Ala Gly Ile Lys Gln Ser Ala Lys Asp His Pro Asn Ala

50 55 60

<210> 77

<211> 192

<212> DNA

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 77

atgattaaaa gagaaaagaa cagaacaatt tcttcccttg gttatgaaga aatttctaat 60

cataaattgc aagaaataca aggtggaaaa ggaattcttg gtaaactagg agtagtacag 120

gcaggagtgg attttgtatc aggagtgtgg gctggaataa aacagtctgc caaagatcat 180

cctaatgcgt aa 192

<210> 78

<211> 75

<212> PRT

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 78

Met Lys Lys Gln Ile Leu Lys Gly Leu Val Ile Val Val Cys Leu Ser

1 5 10 15

Gly Ala Thr Phe Phe Ser Thr Pro Gln Gln Ala Ser Ala Ala Ala Pro

20 25 30

Lys Ile Thr Gln Lys Gln Lys Asn Cys Val Asn Gly Gln Leu Gly Gly

35 40 45

Met Leu Ala Gly Ala Leu Gly Gly Pro Gly Gly Val Val Leu Gly Gly

50 55 60

Ile Gly Gly Ala Ile Ala Gly Gly Cys Phe Asn

65 70 75

<210> 79

<211> 228

<212> DNA

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 79

atgaaaaaac aaattttaaa agggttggtt atagttgttt gtttatctgg ggcaacattt 60

ttctcaacac cacaacaagc ttctgctgct gcaccgaaaa ttactcaaaa acaaaaaaat 120

tgtgttaatg gacaattagg tggaatgctt gctggagctt tgggtggacc tggcggagtt 180

gtgttaggtg gtataggtgg tgcaatagca ggaggttgtt ttaattaa 228

<210> 80

<211> 72

<212> PRT

<213> 耐久肠球菌(Enterococcus durans)

<400> 80

Met Gln Thr Ile Lys Glu Leu Asn Thr Met Glu Leu Gln Glu Ile Ile

1 5 10 15

Gly Gly Glu Asn Asp His Arg Met Pro Tyr Glu Leu Asn Arg Pro Asn

20 25 30

Asn Leu Ser Lys Gly Gly Ala Lys Cys Ala Ala Gly Ile Leu Gly Ala

35 40 45

Gly Leu Gly Ala Val Gly Gly Gly Pro Gly Gly Phe Ile Ser Ala Gly

50 55 60

Ile Ser Ala Val Leu Gly Cys Met

65 70

<210> 81

<211> 219

<212> DNA

<213> 耐久肠球菌(Enterococcus durans)

<400> 81

atgcaaacga tcaaagaatt gaacacgatg gaattacaag aaataattgg aggtgaaaat 60

gaccatcgga tgccttacga attgaaccgt ccaaataatt tatccaaagg tggggctaag 120

tgtgctgctg gaatacttgg cgctggacta ggcgcagtag gcggtggacc tggcggattt 180

attagtgccg gaatcagtgc tgttcttggt tgtatgtaa 219

<210> 82

<211> 72

<212> PRT

<213> 耐久肠球菌(Enterococcus durans)

<400> 82

Met Gln Thr Ile Lys Glu Leu Asn Thr Met Glu Leu Gln Lys Ile Ile

1 5 10 15

Gly Gly Glu Asn Asp His Arg Met Pro Tyr Glu Leu Asn Arg Pro Asn

20 25 30

Asn Leu Ser Lys Gly Gly Ala Lys Cys Ala Ala Gly Ile Leu Gly Ala

35 40 45

Gly Leu Gly Ala Val Gly Gly Gly Pro Gly Gly Phe Ile Ser Ala Gly

50 55 60

Ile Ser Ala Val Leu Gly Cys Met

65 70

<210> 83

<211> 219

<212> DNA

<213> 耐久肠球菌(Enterococcus durans)

<400> 83

atgcaaacga tcaaagaatt gaacacgatg gaattacaaa aaataattgg aggtgaaaat 60

gaccatcgga tgccttacga attgaaccgt ccaaataatt tatccaaagg tggagctaag 120

tgcgctgccg gaatacttgg tgctggatta ggcgcagtag gcggtggacc tggcggattt 180

attagtgccg gaatcagtgc tgttcttggt tgtatgtaa 219

<210> 84

<211> 220

<212> PRT

<213> 停乳链球菌似马亚种(Streptococcus dysgalactiae subsp. equisimilis)

<400> 84

Met Lys Lys Leu Lys Arg Leu Val Ile Ser Leu Val Thr Ser Leu Leu

1 5 10 15

Val Ile Ser Ser Thr Val Pro Ala Leu Val Tyr Ala Asn Glu Thr Asn

20 25 30

Asn Phe Ala Glu Thr Gln Lys Glu Ile Thr Thr Asn Ser Glu Ala Thr

35 40 45

Leu Thr Asn Glu Asp Tyr Thr Lys Leu Thr Ser Glu Val Lys Thr Ile

50 55 60

Tyr Thr Asn Leu Ile Gln Tyr Asp Gln Thr Lys Asn Lys Phe Tyr Val

65 70 75 80

Asp Glu Asp Lys Thr Glu Gln Tyr Tyr Asn Tyr Asp Asp Glu Ser Ile

85 90 95

Lys Gly Val Tyr Leu Met Lys Asp Ser Leu Asn Asp Glu Leu Asn Asn

100 105 110

Asn Asn Ser Ser Asn Tyr Ser Glu Ile Ile Asn Gln Lys Ile Ser Glu

115 120 125

Ile Asp Tyr Val Leu Gln Gly Asn Asp Ile Asn Asn Leu Ile Pro Ser

130 135 140

Asn Thr Arg Val Lys Arg Ser Ala Asp Phe Ser Trp Ile Gln Arg Cys

145 150 155 160

Leu Glu Glu Ala Trp Gly Tyr Ala Ile Ser Leu Val Thr Leu Lys Gly

165 170 175

Ile Ile Asn Leu Phe Lys Ala Gly Lys Phe Glu Ala Ala Ala Ala Lys

180 185 190

Leu Ala Ser Ala Thr Ala Gly Arg Ile Ala Gly Met Ala Ala Leu Phe

195 200 205

Ala Phe Val Ala Thr Cys Gly Ala Thr Thr Val Ser

210 215 220

<210> 85

<211> 663

<212> DNA

<213> 停乳链球菌似马亚种(Streptococcus dysgalactiae subsp. equisimilis)

<400> 85

atgaaaaaat taaaacgtct tgttatctct cttgttactt cattactagt aatttcaagt 60

acagttccag cacttgttta cgctaatgaa acaaataact ttgcagaaac tcaaaaagaa 120

attacaacaa attcagaagc aacattaacc aatgaagact acactaaatt aacttccgaa 180

gtaaaaacaa tttatacaaa tctgattcaa tacgaccaaa caaaaaacaa attttacgtc 240

gatgaagaca aaactgaaca atattataac tacgatgatg aaagtataaa aggggtttat 300

ctcatgaaag atagtttgaa cgatgagtta aacaataata actcttcaaa ctattctgaa 360

ataattaatc aaaaaatctc tgaaattgac tatgtccttc aaggaaacga tataaataat 420

ttaattccta gcaataccag agtaaaaaga tcagcagatt tttcttggat tcaaagatgt 480

ctagaagaag catggggata tgctattagt ctagttactc taaaaggaat aatcaatcta 540

tttaaagcag gaaaatttga agctgctgct gctaaattag cttctgctac agcaggtaga 600

atcgctggaa tggctgcctt atttgctttc gtagcaactt gcggtgcgac aactgtatca 660

taa 663

<210> 86

<211> 57

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 86

Met Lys Gln Tyr Lys Val Leu Asn Glu Lys Glu Met Lys Lys Pro Ile

1 5 10 15

Gly Gly Glu Ser Val Phe Ser Lys Ile Gly Asn Ala Val Gly Pro Ala

20 25 30

Ala Tyr Trp Ile Leu Lys Gly Leu Gly Asn Met Ser Asp Val Asn Gln

35 40 45

Ala Asp Arg Ile Asn Arg Lys Lys His

50 55

<210> 87

<211> 174

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 87

atgaagcaat ataaagtatt gaatgaaaaa gaaatgaaaa aacctattgg gggagagtcg 60

gtttttagta aaataggtaa tgctgtaggt ccagctgctt attggatttt aaaaggatta 120

ggtaatatga gtgatgtaaa ccaagctgat agaattaata gaaagaaaca ttaa 174

<210> 88

<211> 44

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 88

Met Gly Ala Ile Ala Lys Leu Val Ala Lys Phe Gly Trp Pro Ile Val

1 5 10 15

Lys Lys Tyr Tyr Lys Gln Ile Met Gln Phe Ile Gly Glu Gly Trp Ala

20 25 30

Ile Asn Lys Ile Ile Asp Trp Ile Lys Lys His Ile

35 40

<210> 89

<211> 135

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 89

atgggagcaa tcgcaaaatt agtagcaaag tttggatggc caattgttaa aaagtattac 60

aaacaaatta tgcaatttat tggagaagga tgggcaatta acaaaattat tgattggatc 120

aaaaaacata tttaa 135

<210> 90

<211> 43

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 90

Met Gly Ala Ile Ala Lys Leu Val Ala Lys Phe Gly Trp Pro Phe Ile

1 5 10 15

Lys Lys Phe Tyr Lys Gln Ile Met Gln Phe Ile Gly Gln Gly Trp Thr

20 25 30

Ile Asp Gln Ile Glu Lys Trp Leu Lys Arg His

35 40

<210> 91

<211> 132

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 91

atgggagcaa tcgcaaaatt agtagcaaag tttggatggc catttattaa aaaattctac 60

aaacaaatta tgcagtttat cggacaagga tggacaatag atcaaattga aaaatggtta 120

aaaagacatt ga 132

<210> 92

<211> 74

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 92

Met Leu Asn Lys Lys Leu Leu Glu Asn Gly Val Val Asn Ala Val Thr

1 5 10 15

Ile Asp Glu Leu Asp Ala Gln Phe Gly Gly Met Ser Lys Arg Asp Cys

20 25 30

Asn Leu Met Lys Ala Cys Cys Ala Gly Gln Ala Val Thr Tyr Ala Ile

35 40 45

His Ser Leu Leu Asn Arg Leu Gly Gly Asp Ser Ser Asp Pro Ala Gly

50 55 60

Cys Asn Asp Ile Val Arg Lys Tyr Cys Lys

65 70

<210> 93

<211> 225

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 93

atgttaaata aaaaattatt agaaaatggt gtagtaaatg ctgtaacaat tgatgaactt 60

gatgctcaat ttggtggaat gagcaaacgt gattgtaact tgatgaaggc gtgttgtgct 120

ggacaagcag taacatatgc tattcatagt cttttaaatc gattaggtgg agactctagt 180

gatccagctg gttgtaatga tattgtaaga aaatattgta aataa 225

<210> 94

<211> 65

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 94

Met Lys His Leu Lys Ile Leu Ser Ile Lys Glu Thr Gln Leu Ile Tyr

1 5 10 15

Gly Gly Thr Thr His Ser Gly Lys Tyr Tyr Gly Asn Gly Val Tyr Cys

20 25 30

Thr Lys Asn Lys Cys Thr Val Asp Trp Ala Lys Ala Thr Thr Cys Ile

35 40 45

Ala Gly Met Ser Ile Gly Gly Phe Leu Gly Gly Ala Ile Pro Gly Lys

50 55 60

Cys

65

<210> 95

<211> 195

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 95

atgaaacatt taaaaatttt gtctattaaa gagacacaac ttatctatgg gggtaccact 60

catagtggaa aatattatgg aaatggagtg tattgcacta aaaataaatg tacggtcgat 120

tgggccaagg caactacttg tattgcagga atgtctatag gtggtttttt aggtggagca 180

attccaggga agtgc 195

<210> 96

<211> 105

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 96

Met Val Lys Glu Asn Lys Phe Ser Lys Ile Phe Ile Leu Met Ala Leu

1 5 10 15

Ser Phe Leu Gly Leu Ala Leu Phe Ser Ala Ser Leu Gln Phe Leu Pro

20 25 30

Ile Ala His Met Ala Lys Glu Phe Gly Ile Pro Ala Ala Val Ala Gly

35 40 45

Thr Val Leu Asn Val Val Glu Ala Gly Gly Trp Val Thr Thr Ile Val

50 55 60

Ser Ile Leu Thr Ala Val Gly Ser Gly Gly Leu Ser Leu Leu Ala Ala

65 70 75 80

Ala Gly Arg Glu Ser Ile Lys Ala Tyr Leu Lys Lys Glu Ile Lys Lys

85 90 95

Lys Gly Lys Arg Ala Val Ile Ala Trp

100 105

<210> 97

<211> 318

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 97

atggttaaag aaaataaatt ttctaagatt tttattttaa tggctttgag ttttttgggg 60

ttagccttgt ttagtgcaag tcttcagttt ttgcccattg cacatatggc taaagagttc 120

ggtataccag cagcagttgc aggaactgtg cttaatgtag ttgaagctgg tggatgggtc 180

actactattg tatcaattct tactgctgta ggtagcggag gtctttcttt actcgctgca 240

gcaggaagag agtcaattaa agcatacctt aagaaagaaa ttaagaaaaa aggaaaaaga 300

gcagttattg cttggtaa 318

<210> 98

<211> 71

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 98

Met Gln Asn Val Lys Glu Leu Ser Thr Lys Glu Met Lys Gln Ile Ile

1 5 10 15

Gly Gly Glu Asn Asp His Arg Met Pro Asn Glu Leu Asn Arg Pro Asn

20 25 30

Asn Leu Ser Lys Gly Gly Ala Lys Cys Gly Ala Ala Ile Ala Gly Gly

35 40 45

Leu Phe Gly Ile Pro Lys Gly Pro Leu Ala Trp Ala Ala Gly Leu Ala

50 55 60

Asn Val Tyr Ser Lys Cys Asn

65 70

<210> 99

<211> 216

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 99

atgcaaaatg taaaagaatt aagtacgaaa gagatgaaac aaattatcgg tggagaaaat 60

gatcacagaa tgcctaatga gttaaataga cctaacaact tatctaaagg tggagcaaaa 120

tgtggtgctg caattgctgg gggattattt ggaatcccaa aaggaccact agcatgggct 180

gctgggttag caaatgtata ctctaaatgc aactaa 216

<210> 100

<211> 58

<212> PRT

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 100

Met Lys Lys Leu Thr Ser Lys Glu Met Ala Gln Val Val Gly Gly Lys

1 5 10 15

Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val Asp

20 25 30

Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala Asn Leu

35 40 45

Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser

50 55

<210> 101

<211> 177

<212> DNA

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 101

ttgaagaaat taacatcaaa agaaatggca caagtagtag gtggaaaata ctacggtaat 60

ggagtctcat gtaataaaaa agggtgcagt gttgattggg gaaaagctat tggcattatt 120

ggaaataatt ctgctgcgaa tttagctact ggtggagcag ctggttggaa aagttaa 177

<210> 102

<211> 44

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 102

Met Leu Ala Lys Ile Lys Ala Met Ile Lys Lys Phe Pro Asn Pro Tyr

1 5 10 15

Thr Leu Ala Ala Lys Leu Thr Thr Tyr Glu Ile Asn Trp Tyr Lys Gln

20 25 30

Gln Tyr Gly Arg Tyr Pro Trp Glu Arg Pro Val Ala

35 40

<210> 103

<211> 135

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 103

atgttagcaa aaattaaagc gatgattaag aagtttccga acccttatac tttagcagct 60

aagctaacga cttacgaaat taattggtat aaacaacaat acggtcgtta tccttgggag 120

cgccctgtag cataa 135

<210> 104

<211> 71

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 104

Met Arg Lys Lys Leu Phe Ser Leu Ala Leu Ile Gly Ile Phe Gly Leu

1 5 10 15

Val Val Thr Asn Phe Gly Thr Lys Val Asp Ala Ala Thr Arg Ser Tyr

20 25 30

Gly Asn Gly Val Tyr Cys Asn Asn Ser Lys Cys Trp Val Asn Trp Gly

35 40 45

Glu Ala Lys Glu Asn Ile Ala Gly Ile Val Ile Ser Gly Trp Ala Ser

50 55 60

Gly Leu Ala Gly Met Gly His

65 70

<210> 105

<211> 216

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 105

atgagaaaaa aattatttag tttagctctt attggaatat ttgggttagt tgtgacaaat 60

tttggtacaa aagttgatgc agctacgcgt tcatatggta atggtgttta ttgtaataat 120

agtaaatgct gggttaactg gggagaagct aaagagaata ttgcaggaat cgttattagt 180

ggctgggctt ctggtttggc aggtatggga cattaa 216

<210> 106

<211> 34

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 106

Met Asn Phe Leu Lys Asn Gly Ile Ala Lys Trp Met Thr Gly Ala Glu

1 5 10 15

Leu Gln Ala Tyr Lys Lys Lys Tyr Gly Cys Leu Pro Trp Glu Lys Ile

20 25 30

Ser Cys

<210> 107

<211> 105

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 107

atgaattttc ttaaaaatgg tatcgcaaaa tggatgaccg gtgctgaatt gcaagcgtat 60

aaaaagaaat atggatgctt gccatgggaa aaaatttctt gttaa 105

<210> 108

<211> 76

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 108

Met Lys Lys Lys Leu Val Lys Gly Leu Val Ile Cys Gly Met Ile Gly

1 5 10 15

Ile Gly Phe Thr Ala Leu Gly Thr Asn Val Glu Ala Ala Thr Tyr Tyr

20 25 30

Gly Asn Gly Val Tyr Cys Asn Lys Gln Lys Cys Trp Val Asp Trp Ser

35 40 45

Arg Ala Arg Ser Glu Ile Ile Asp Arg Gly Val Lys Ala Tyr Val Asn

50 55 60

Gly Phe Thr Lys Val Leu Gly Gly Ile Gly Gly Arg

65 70 75

<210> 109

<211> 231

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 109

atgaaaaaga aattagttaa aggcttagtt atttgtggca tgattgggat tggttttaca 60

gcattaggaa caaatgtaga agccgccacg tattacggaa atggtgtcta ttgcaataag 120

caaaaatgtt gggtagattg gagtagagca cgttctgaaa ttatagacag aggcgtaaaa 180

gcatacgtca atggatttac gaaagtgtta ggtggtatag gtggaagata a 231

<210> 110

<211> 60

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 110

Met Lys Lys Glu Glu Leu Val Gly Met Ala Lys Glu Asp Phe Leu Asn

1 5 10 15

Val Ile Cys Glu Asn Asp Asn Lys Leu Glu Asn Ser Gly Ala Lys Cys

20 25 30

Pro Trp Trp Asn Leu Ser Cys His Leu Gly Asn Asp Gly Lys Ile Cys

35 40 45

Thr Tyr Ser His Glu Cys Thr Ala Gly Cys Asn Ala

50 55 60

<210> 111

<211> 183

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 111

atgaaaaaag aagaattagt aggaatggct aaggaagact ttttaaatgt tatttgtgaa 60

aatgacaaca aactagaaaa tagtggagca aaatgtcctt ggtggaatct ttcttgtcat 120

ttaggcaatg atggtaaaat ttgcacttat tcacatgaat gtaccgcagg ttgtaatgca 180

taa 183

<210> 112

<211> 61

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 112

Met Thr Glu Leu Asn Lys Arg Leu Gln Leu Lys Arg Asp Val Ser Thr

1 5 10 15

Glu Asn Ser Leu Lys Lys Ile Ser Asn Thr Asp Glu Thr His Gly Gly

20 25 30

Val Thr Thr Ser Ile Pro Cys Thr Val Met Val Ser Ala Ala Val Cys

35 40 45

Pro Thr Leu Val Cys Ser Asn Lys Cys Gly Gly Arg Gly

50 55 60

<210> 113

<211> 186

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 113

atgactgaac ttaacaaaag attacaatta aaaagagatg tttcaacaga aaatagtttg 60

aaaaaaattt ctaatactga tgaaacacat gggggagtta ctacatcaat tccatgtaca 120

gtaatggtta gtgcggcagt atgtcctacc cttgtttgct cgaataaatg tggcggtaga 180

ggctag 186

<210> 114

<211> 58

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 114

Met Gln Asn Val Lys Glu Val Ser Val Lys Glu Met Lys Gln Ile Ile

1 5 10 15

Gly Gly Ser Asn Asp Ser Leu Trp Tyr Gly Val Gly Gln Phe Met Gly

20 25 30

Lys Gln Ala Asn Cys Ile Thr Asn His Pro Val Lys His Met Ile Ile

35 40 45

Pro Gly Tyr Cys Leu Ser Lys Ile Leu Gly

50 55

<210> 115

<211> 177

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 115

atgcaaaatg taaaagaagt ttctgtaaaa gagatgaaac aaattatcgg tggttctaat 60

gatagtcttt ggtatggtgt aggacaattt atgggtaaac aagcaaactg tataacaaac 120

catcctgtta aacacatgat aattcctgga tattgtttat cgaaaatttt agggtaa 177

<210> 116

<211> 55

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 116

Met Lys Lys Tyr Asn Glu Leu Ser Lys Lys Glu Leu Leu Gln Ile Gln

1 5 10 15

Gly Gly Ile Ala Pro Ile Ile Val Ala Gly Leu Gly Tyr Leu Val Lys

20 25 30

Asp Ala Trp Asp His Ser Asp Gln Ile Ile Ser Gly Phe Lys Lys Gly

35 40 45

Trp Asn Gly Gly Arg Arg Lys

50 55

<210> 117

<211> 168

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 117

atgaaaaaat ataatgagtt atctaaaaaa gaacttctac agattcaagg aggaatagca 60

cctattatag ttgctggcct tggctattta gtaaaagatg catgggatca ctcagatcaa 120

ataatctcag gatttaaaaa aggttggaat ggtggacgta gaaaataa 168

<210> 118

<211> 343

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 118

Met Lys Asn Ile Leu Leu Ser Ile Leu Gly Val Leu Ser Ile Val Val

1 5 10 15

Ser Leu Ala Phe Ser Ser Tyr Ser Val Asn Ala Ala Ser Asn Glu Trp

20 25 30

Ser Trp Pro Leu Gly Lys Pro Tyr Ala Gly Arg Tyr Glu Glu Gly Gln

35 40 45

Gln Phe Gly Asn Thr Ala Phe Asn Arg Gly Gly Thr Tyr Phe His Asp

50 55 60

Gly Phe Asp Phe Gly Ser Ala Ile Tyr Gly Asn Gly Ser Val Tyr Ala

65 70 75 80

Val His Asp Gly Lys Ile Leu Tyr Ala Gly Trp Asp Pro Val Gly Gly

85 90 95

Gly Ser Leu Gly Ala Phe Ile Val Leu Gln Ala Gly Asn Thr Asn Val

100 105 110

Ile Tyr Gln Glu Phe Ser Arg Asn Val Gly Asp Ile Lys Val Ser Thr

115 120 125

Gly Gln Thr Val Lys Lys Gly Gln Leu Ile Gly Lys Phe Thr Ser Ser

130 135 140

His Leu His Leu Gly Met Thr Lys Lys Glu Trp Arg Ser Ala His Ser

145 150 155 160

Ser Trp Asn Lys Asp Asp Gly Thr Trp Phe Asn Pro Ile Pro Ile Leu

165 170 175

Gln Gly Gly Ser Thr Pro Thr Pro Pro Asn Pro Gly Pro Lys Asn Phe

180 185 190

Thr Thr Asn Val Arg Tyr Gly Leu Arg Val Leu Gly Gly Ser Trp Leu

195 200 205

Pro Glu Val Thr Asn Phe Asn Asn Thr Asn Asp Gly Phe Ala Gly Tyr

210 215 220

Pro Asn Arg Gln His Asp Met Leu Tyr Ile Lys Val Asp Lys Gly Gln

225 230 235 240

Met Lys Tyr Arg Val His Thr Ala Gln Ser Gly Trp Leu Pro Trp Val

245 250 255

Ser Lys Gly Asp Lys Ser Asp Thr Val Asn Gly Ala Ala Gly Met Pro

260 265 270

Gly Gln Ala Ile Asp Gly Val Gln Leu Asn Tyr Ile Thr Pro Lys Gly

275 280 285

Glu Lys Leu Ser Gln Ala Tyr Tyr Arg Ser Gln Thr Thr Lys Arg Ser

290 295 300

Gly Trp Leu Lys Val Ser Ala Asp Asn Gly Ser Ile Pro Gly Leu Asp

305 310 315 320

Ser Tyr Ala Gly Ile Phe Gly Glu Pro Leu Asp Arg Leu Gln Ile Gly

325 330 335

Ile Ser Gln Ser Asn Pro Phe

340

<210> 119

<211> 1032

<212> DNA

<213> 粪肠球菌(Enterococcus faecalis)

<400> 119

atgaaaaata ttttactttc tattctaggg gtattatcta tcgttgtttc tttggcgttt 60

tcttcttatt ctgtcaacgc agcttctaat gagtggtcgt ggccactggg caaaccatat 120

gcgggaagat atgaagaagg acaacaattc gggaacactg catttaaccg aggaggtact 180

tatttccatg atgggtttga ctttggttct gctatttatg gaaatggcag tgtgtatgct 240

gtgcatgatg gtaaaatttt atatgctggt tgggatcctg taggtggagg ctcattaggt 300

gcatttattg tactacaagc gggaaacaca aatgtgattt atcaagaatt tagccgaaat 360

gttggagata ttaaagttag cactggacaa actgttaaaa aaggacagct gataggaaag 420

tttacttcta gtcatttaca tttaggaatg acaaaaaaag aatggcgttc tgctcattct 480

tcttggaata aagatgatgg cacttggttt aacccaattc ctatacttca aggaggatct 540

acgcctacgc ctccaaatcc aggaccaaaa aatttcacaa caaatgttcg ttacggattg 600

cgggtcctcg gaggttcatg gttaccagaa gtaaccaact ttaacaatac caatgatggt 660

ttcgcaggtt accctaatcg tcaacatgat atgctttata taaaggtaga taaagggcaa 720

atgaaatatc gtgttcacac ggctcaaagt ggatggttgc cttgggtaag taaaggggat 780

aagagcgata cagtaaatgg agcggcaggt atgcctggac aagcaattga tggtgttcag 840

ctaaactata taactcctaa gggagaaaaa ttatcacagg cttactatcg ttcacaaact 900

acgaaacgat caggctggtt aaaagtaagt gcagataatg gttctattcc tggactagac 960

agttatgcag gaatctttgg agaaccgttg gatcgcttgc aaataggtat ttcacagtca 1020

aatccatttt aa 1032

<210> 120

<211> 56

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 120

Met Glu Asn Lys Lys Asp Leu Phe Asp Leu Glu Ile Lys Lys Asp Asn

1 5 10 15

Met Glu Asn Asn Asn Glu Leu Glu Ala Gln Ser Leu Gly Pro Ala Ile

20 25 30

Lys Ala Thr Arg Gln Val Cys Pro Lys Ala Thr Arg Phe Val Thr Val

35 40 45

Ser Cys Lys Lys Ser Asp Cys Gln

50 55

<210> 121

<211> 171

<212> DNA

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 121

atggaaaaca aaaaagattt atttgattta gaaatcaaaa aagataatat ggaaaataat 60

aatgaattag aagctcaatc tcttggtcct gcaattaagg caactagaca ggtatgtcct 120

aaagcaacac gttttgttac agtttcttgt aaaaaaagtg attgtcaata g 171

<210> 122

<211> 51

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 122

Met Ala Ala Phe Met Lys Leu Ile Gln Phe Leu Ala Thr Lys Gly Gln

1 5 10 15

Lys Tyr Val Ser Leu Ala Trp Lys His Lys Gly Thr Ile Leu Lys Trp

20 25 30

Ile Asn Ala Gly Gln Ser Phe Glu Trp Ile Tyr Lys Gln Ile Lys Lys

35 40 45

Leu Trp Ala

50

<210> 123

<211> 156

<212> DNA

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 123

atggcagcat ttatgaagtt aattcagttc ttagcaacta aaggtcaaaa gtatgtttca 60

cttgcatgga aacataaagg tactatttta aaatggatta acgccggtca aagttttgaa 120

tggatttata aacaaatcaa aaaattatgg gcataa 156

<210> 124

<211> 52

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 124

Met Glu Ala Val Lys Glu Lys Asn Asp Leu Phe Asn Leu Asp Val Lys

1 5 10 15

Val Asn Ala Lys Glu Ser Asn Asp Ser Gly Ala Glu Pro Arg Ile Ala

20 25 30

Ser Lys Phe Ile Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser Phe Asn

35 40 45

Ser Tyr Cys Cys

50

<210> 125

<211> 159

<212> DNA

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 125

atggaagcag taaaagaaaa aaatgatctt tttaatcttg atgttaaagt taatgcaaaa 60

gaatctaacg attcaggagc tgaaccaaga attgctagta aatttatatg tactcctgga 120

tgtgcaaaaa caggtagttt taacagttat tgttgttaa 159

<210> 126

<211> 55

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 126

Met Asn Asn Ser Leu Phe Asp Leu Asn Leu Asn Lys Gly Val Glu Thr

1 5 10 15

Gln Lys Ser Asp Leu Ser Pro Gln Ser Ala Ser Val Leu Lys Thr Ser

20 25 30

Ile Lys Val Ser Lys Lys Tyr Cys Lys Gly Val Thr Leu Thr Cys Gly

35 40 45

Cys Asn Ile Thr Gly Gly Lys

50 55

<210> 127

<211> 168

<212> DNA

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 127

atgaataact cattattcga tttaaaccta aacaaaggtg tagaaactca aaagagtgat 60

ttaagtccgc aatctgctag tgtcttgaag acttctatta aagtatctaa aaaatattgt 120

aaaggtgtta ctttaacatg cggttgcaat attactggtg gtaaataa 168

<210> 128

<211> 52

<212> PRT

<213> 鸡葡萄球菌(Staphylococcus gallinarum)

<400> 128

Met Glu Ala Val Lys Glu Lys Asn Glu Leu Phe Asp Leu Asp Val Lys

1 5 10 15

Val Asn Ala Lys Glu Ser Asn Asp Ser Gly Ala Glu Pro Arg Ile Ala

20 25 30

Ser Lys Phe Leu Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser Phe Asn

35 40 45

Ser Tyr Cys Cys

50

<210> 129

<211> 159

<212> DNA

<213> 鸡葡萄球菌(Staphylococcus gallinarum)

<400> 129

atggaagcag taaaagagaa aaatgaactt tttgatcttg acgttaaagt aaatgcaaaa 60

gagtctaatg attcaggcgc agaaccacga attgctagta aatttttatg tactcctgga 120

tgtgccaaaa caggtagctt caatagctac tgttgttaa 159

<210> 130

<211> 63

<212> PRT

<213> 格氏乳球菌(Lactococcus garvieae)

<400> 130

Met Glu Asn Asn Asn Tyr Thr Val Leu Ser Asp Glu Glu Leu Gln Lys

1 5 10 15

Ile Asp Gly Gly Ile Gly Gly Ala Leu Gly Asn Ala Leu Asn Gly Leu

20 25 30

Gly Thr Trp Ala Asn Met Met Asn Gly Gly Gly Phe Val Asn Gln Trp

35 40 45

Gln Val Tyr Ala Asn Lys Gly Lys Ile Asn Gln Tyr Arg Pro Tyr

50 55 60

<210> 131

<211> 192

<212> DNA

<213> 格氏乳球菌(Lactococcus garvieae)

<400> 131

atggaaaaca acaattacac agtactttca gatgaagaac tacaaaaaat tgatggtgga 60

atcggcgggg ctcttggtaa tgctctcaac ggattaggta cctgggcaaa catgatgaac 120

ggtggaggat ttgttaatca gtggcaagtt tatgctaata aaggaaaaat aaatcaatac 180

cgtccgtatt aa 192

<210> 132

<211> 63

<212> PRT

<213> 格氏乳球菌(Lactococcus garvieae)

<400> 132

Met Phe Asp Leu Val Ala Thr Gly Met Ala Ala Gly Val Ala Lys Thr

1 5 10 15

Ile Val Asn Ala Val Ser Ala Gly Met Asp Ile Ala Thr Ala Leu Ser

20 25 30

Leu Phe Ser Gly Ala Phe Thr Ala Ala Gly Gly Ile Met Ala Leu Ile

35 40 45

Lys Lys Tyr Ala Gln Lys Lys Leu Trp Lys Gln Leu Ile Ala Ala

50 55 60

<210> 133

<211> 192

<212> DNA

<213> 格氏乳球菌(Lactococcus garvieae)

<400> 133

atgtttgatt tagtcgcgac tggaatggct gcaggtgtag caaaaactat tgttaatgcc 60

gttagtgctg gtatggatat tgccactgct ttatcattgt tctcaggagc ttttactgca 120

gctgggggaa ttatggcact cattaaaaaa tatgctcaaa agaaattatg gaaacagctt 180

attgctgcat aa 192

<210> 134

<211> 91

<212> PRT

<213> 格氏乳杆菌(Lactobacillus gasseri)

<400> 134

Met Val Thr Lys Tyr Gly Arg Asn Leu Gly Leu Asn Lys Val Glu Leu

1 5 10 15

Phe Ala Ile Trp Ala Val Leu Val Val Ala Leu Leu Leu Thr Thr Ala

20 25 30

Asn Ile Tyr Trp Ile Ala Asp Gln Phe Gly Ile His Leu Ala Thr Gly

35 40 45

Thr Ala Arg Lys Leu Leu Asp Ala Met Ala Ser Gly Ala Ser Leu Gly

50 55 60

Thr Ala Phe Ala Ala Ile Leu Gly Val Thr Leu Pro Ala Trp Ala Leu

65 70 75 80

Ala Ala Ala Gly Ala Leu Gly Ala Thr Ala Ala

85 90

<210> 135

<211> 276

<212> DNA

<213> 格氏乳杆菌(Lactobacillus gasseri)

<400> 135

atggttacta agtacggacg taatttaggt ttgaacaagg tagagttgtt tgcaatttgg 60

gcggttttag tagttgctct tttattgacc acagcgaaca tttattggat tgctgatcaa 120

ttcgggattc atttagcgac tggaacagcc cgtaagttat tagatgcaat ggcttctggt 180

gcctcattgg gaactgcctt tgctgctatt ttgggcgtga cattacctgc atgggctttg 240

gcagctgcag gagcattggg agcgactgca gcctag 276

<210> 136

<211> 75

<212> PRT

<213> 格氏乳杆菌(Lactobacillus gasseri)

<400> 136

Met Lys Asn Phe Asn Thr Leu Ser Phe Glu Thr Leu Ala Asn Ile Val

1 5 10 15

Gly Gly Arg Asn Asn Trp Ala Ala Asn Ile Gly Gly Val Gly Gly Ala

20 25 30

Thr Val Ala Gly Trp Ala Leu Gly Asn Ala Val Cys Gly Pro Ala Cys

35 40 45

Gly Phe Val Gly Ala His Tyr Val Pro Ile Ala Trp Ala Gly Val Thr

50 55 60

Ala Ala Thr Gly Gly Phe Gly Lys Ile Arg Lys

65 70 75

<210> 137

<211> 228

<212> DNA

<213> 格氏乳杆菌(Lactobacillus gasseri)

<400> 137

atgaaaaatt ttaatacatt atcatttgaa acattggcta acatagttgg tgggagaaat 60

aattgggctg ctaatatagg tggagtaggt ggagcgacag tcgctggatg ggctcttgga 120

aatgcagttt gcggtcctgc ttgtggcttt gttggagcac actatgttcc aatagcatgg 180

gctggcgtaa cggcagctac tggtggattc ggaaagataa gaaagtag 228

<210> 138

<211> 64

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 138

Met Ser Lys Leu Val Lys Thr Leu Thr Ile Ser Glu Ile Ser Lys Ala

1 5 10 15

Gln Asn Asn Gly Gly Lys Pro Ala Trp Cys Trp Tyr Thr Leu Ala Met

20 25 30

Cys Gly Ala Gly Tyr Asp Ser Gly Thr Cys Asp Tyr Met Tyr Ser His

35 40 45

Cys Phe Gly Ile Lys His His Ser Ser Gly Ser Ser Ser Tyr His Cys

50 55 60

<210> 139

<211> 195

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 139

atgagtaaat tggttaagac acttactata agtgaaattt ctaaggctca aaacaacggt 60

ggaaaacctg catggtgttg gtatacttta gcaatgtgtg gtgctggtta tgattcggga 120

acctgtgatt atatgtattc gcattgtttt ggtataaagc atcatagtag tggtagtagc 180

agttatcatt gttag 195

<210> 140

<211> 359

<212> PRT

<213> 地中海富盐菌(Haloferax mediterranei)

<400> 140

Met Ser Lys Asp Arg Asp Gly Arg Arg Thr Ser Arg Arg Gly Thr Leu

1 5 10 15

Lys Lys Ile Gly Gly Phe Ser Leu Gly Ala Leu Ser Phe Gly Ala Val

20 25 30

Gly Arg Thr Gln Ala Ala Thr Gly Ser Ser Val Thr Thr Ala Asp Ile

35 40 45

Ala Pro Pro Gly Pro Asn Gly Asp Pro Lys Ser Val Gln Ile Asp Asp

50 55 60

Lys Tyr Thr Gly Ala Glu Met Tyr Gly Glu Gly Asp Phe Arg Val Gly

65 70 75 80

Leu Gly Thr Asp Leu Thr Met Tyr Pro Pro Val Tyr Arg Glu Ser Leu

85 90 95

Gly Asn Gly Ser Gly Gly Trp Glu Phe Asp Phe Thr Val Cys Gly Ser

100 105 110

Thr Ala Cys Arg Phe Val Asp Ser Asn Gly Asp Val Lys Glu Asp Asp

115 120 125

Lys Ala Lys Glu Met Trp Trp Gln Glu Ile Asn Phe Asn Asp Ile Asn

130 135 140

Gln Asp Leu Tyr Ser Arg Asn Asp Ser Asp Trp Val Gly Ser Thr Pro

145 150 155 160

Ala Asp Thr Gln Pro Glu Phe Asp Tyr Thr Glu Phe Ala Leu Ala Arg

165 170 175

Asp Gly Val Thr Leu Ala Leu Thr Ala Leu Asn Pro Ala Met Gly Ser

180 185 190

Leu Ala Leu Gly Ala Thr Tyr Phe Leu Ser Asp Met Val Asn Trp Ile

195 200 205

Ala Ser Gln His Glu Asp Asp Ser Ser Leu Lys Arg Lys Trp Asp Tyr

210 215 220

Asp Gly Leu Ser Gly Pro Leu Tyr Ala Asp Ser Ser Thr Tyr Leu Leu

225 230 235 240

Ala Arg Asp Glu Met Thr Ser Asn Ser Tyr Glu Ser Phe Thr Ile Asp

245 250 255

Asn Ile Ala Val Ala Phe Pro Glu Phe Pro Val Arg Thr Lys Tyr Tyr

260 265 270

Val Thr Phe Thr Ala Pro Asp Asp Pro Ser Thr Gln Ser Ile Ser Thr

275 280 285

Leu Glu Glu Glu Gly Ile Tyr Arg Val Pro Ala Thr Glu Val Ala Ala

290 295 300

Ala Arg Pro Pro Gly Ser Arg Arg Ser Lys Ser Ala Ala Asp Glu Met

305 310 315 320

Val Tyr Val Ala Asp Pro Lys Lys Phe Ile Glu Val Glu Pro Val Lys

325 330 335

Asn Pro Ser Ile Pro Asp Arg Ile Tyr Glu Glu Ile Glu Gln Lys Lys

340 345 350

Lys Gln Arg Ser Arg Lys Gln

355

<210> 141

<211> 1080

<212> DNA

<213> 地中海富盐菌(Haloferax mediterranei)

<400> 141

atgtcgaaag acagagatgg gagaaggaca agtcggcgag gcacgttaaa gaaaatcggc 60

ggtttcagtc tcggagcgct tagtttcggg gcagtcggac gaactcaagc ggcgaccggc 120

tcatcggtta cgaccgctga tatcgcacct cccggaccga acggagaccc gaagagtgtt 180

cagatagatg ataaatacac cggagccgag atgtacggcg agggtgactt cagagtcggt 240

ctcggaactg acctgacgat gtatccgccc gtgtaccgtg agagtcttgg aaatggaagc 300

gggggttggg aattcgactt caccgtttgt gggtccactg cctgtcgatt tgtggacagt 360

aacggtgacg tcaaagagga cgacaaggcg aaagaaatgt ggtggcagga aattaacttc 420

aacgacataa atcaggattt atacagtcgg aacgattccg actgggtcgg gtcgacccct 480

gccgataccc aaccggagtt cgattacacc gactttgcgc tcgctcggga cggagtgacg 540

ctcgctctca cggcactcaa ccccgcaatg gggagtcttg cactcggtgc cacgtacttc 600

ctcagcgaca tggtgaactg gattgcgagc cagcacgaag acgacagttc gctcaagaga 660

aaatgggatt acgacgggct aagtgggccg ttgtacgccg attcgtcgac gtacctactg 720

gcacgcgacg agatgacttc gaactcgtac gaatcattca cgatcgataa catcgccgtt 780

gccttcccag agttccccgt ccggaccaag tactacgtca cattcactgc gccggatgac 840

ccgtcaacgc agtcgatatc tacgctcgaa gaggagggaa tctaccgagt gcccgctacg 900

gaagtggctg cggccagacc accggggtcc cgacgttcca aatcggcagc cgacgagatg 960

gtgtacgttg ccgatccgaa gaagttcata gaggtcgagc cggtgaagaa cccaagtatc 1020

ccggaccgaa tctacgagga gatagagcaa aaaaagaaac aacggagtag gaaacagtag 1080

<210> 142

<211> 311

<212> PRT

<213> Haloarchaeon S8a

<400> 142

Met Ser Asp Lys Asp Ser Ile Asn Arg Arg Asn Val Leu Arg Lys Ile

1 5 10 15

Gly Gly Ile Gly Val Ala Ser Ala Val Gly Phe Ser Gly Leu Ala Ser

20 25 30

Gly Glu Ser Leu Ser Asp Asp Glu Lys Gln Asp Val Ile Asp Thr Ile

35 40 45

Tyr Lys Ser Gln Arg Val Glu Gln Ile Lys Lys Lys Phe Gly Gly Val

50 55 60

Asn Ile Glu Pro Lys Lys Val Gln Ser Val Thr Thr Asn Gln Ser Gly

65 70 75 80

Asp Leu Val Thr Ala Lys Leu Ser Val Ser Asp Gly Asp Leu Val Tyr

85 90 95

Ser Ser Val Lys Asp Thr Thr Val Ile Val Gln Phe Asp Arg Ser Ala

100 105 110

Ser Glu Ile Gly Glu Ser Trp Pro Lys Asn Thr Glu Ala Phe Ile Lys

115 120 125

Ser Thr Ser Ser Gly Val Asp Leu Leu Arg Thr Ala Thr Asp Glu Glu

130 135 140

Ile Lys Asp Val Thr Glu Gly Val Asn Thr Ser Glu Ile Glu Ser Ala

145 150 155 160

Asp Ala Val Asn Ile Phe Ile Asp Pro Glu Ser Gln Thr Tyr Tyr Met

165 170 175

Glu Lys Tyr Asp Phe Asn Asn Lys Val Leu Glu Met Phe Glu Leu Ala

180 185 190

Thr Gly Gly Thr Ser Ser Gly Lys Ile Ser Pro Thr Arg Glu Asp Gln

195 200 205

Asn His Glu Tyr Asn Val Arg Glu His Lys Val Phe Asn Ser Glu Lys

210 215 220

Gln Asn Ile Gln Leu Gln Ser Asp Cys Asn Ile Asn Ser Asn Thr Ala

225 230 235 240

Ala Asp Val Ile Leu Cys Phe Asn Gln Val Gly Ser Cys Ala Leu Cys

245 250 255

Ser Pro Thr Leu Val Gly Gly Pro Val Pro Thr Val Ala Cys Leu Leu

260 265 270

Val Val Cys Phe Gly Thr Pro Asn Ala Val Ser Ala Ile Leu Glu Glu

275 280 285

Val Asp Asn Ser Cys Phe Asn Leu Ile Lys Asp Val Ile Ser Cys Trp

290 295 300

Asp Glu Trp Thr Ser Phe Trp

305 310

<210> 143

<211> 936

<212> DNA

<213> Haloarchaeon S8a

<400> 143

atgtcggata aagacagcat taacagaaga aatgtattaa gaaaaattgg cggtatcggt 60

gtggcttcag ctgtcggatt ttctggtttg gcaagcgggg aaagtcttag cgatgatgag 120

aaacaagatg ttattgacac aatttacaaa tcacaaagag ttgaacagat aaagaaaaag 180

ttcggaggag tgaatattga gccgaaaaag gttcaatctg taacgaccaa tcagagcgga 240

gatcttgtta cggcgaagct gtcggttagt gatggggatt tggtatattc gagtgtcaaa 300

gatacaactg taatagttca gttcgataga tcggcttctg aaattggtga aagttggccc 360

aagaatactg aggcattcat caaatcgacg tcctctgggg tcgatcttct acgtacagca 420

actgatgaag aaataaagga cgttactgag ggagtcaaca catctgaaat tgaatctgcg 480

gatgctgtta acatatttat tgatcctgaa tcacagacat actatatgga gaaatatgac 540

tttaataata aggtacttga gatgtttgaa ttagcgacag gtgggacaag tagtggtaaa 600

atctccccca cacgtgaaga ccagaatcac gaatataatg ttagggaaca taaagtattt 660

aactcagaaa aacagaatat acaacttcag agtgactgta atataaacag taacaccgct 720

gctgatgtta ttctatgctt caaccaggtt ggttcttgtg cactctgctc cccgacttta 780

gtcggaggtc cagtccctac agttgcatgt ctcttagtcg tctgtttcgg cactccaaat 840

gctgtgtccg cgatacttga agaagtcgat aattcttgct ttaacttgat caaggatgta 900

atttcgtgtt gggatgaatg gactagcttc tggtga 936

<210> 144

<211> 333

<212> PRT

<213> 瑞士乳杆菌(Lactobacillus helveticus)

<400> 144

Met Lys His Leu Asn Glu Thr Thr Asn Val Arg Ile Leu Ser Gln Phe

1 5 10 15

Asp Met Asp Thr Gly Tyr Gln Ala Val Val Gln Lys Gly Asn Val Gly

20 25 30

Ser Lys Tyr Val Tyr Gly Leu Gln Leu Arg Lys Gly Ala Thr Thr Ile

35 40 45

Leu Arg Gly Tyr Arg Gly Ser Lys Ile Asn Asn Pro Ile Leu Glu Leu

50 55 60

Ser Gly Gln Ala Gly Gly His Thr Gln Thr Trp Glu Phe Ala Gly Asp

65 70 75 80

Arg Lys Asp Ile Asn Gly Glu Glu Arg Ala Gly Gln Trp Phe Ile Gly

85 90 95

Val Lys Pro Ser Lys Ile Glu Gly Ser Lys Ile Ile Trp Ala Lys Gln

100 105 110

Ile Ala Arg Val Asp Leu Arg Asn Gln Met Gly Pro His Tyr Ser Asn

115 120 125

Thr Asp Phe Pro Arg Leu Ser Tyr Leu Asn Arg Ala Gly Ser Asn Pro

130 135 140

Phe Ala Gly Asn Lys Met Thr His Ala Glu Ala Ala Val Ser Pro Asp

145 150 155 160

Tyr Thr Lys Phe Leu Ile Ala Thr Val Glu Asn Asn Cys Ile Gly His

165 170 175

Phe Thr Ile Tyr Asn Leu Asp Thr Ile Asn Glu Lys Leu Asp Glu Lys

180 185 190

Gly Asn Ser Glu Asp Val Asn Leu Glu Thr Val Lys Tyr Glu Asp Ser

195 200 205

Phe Ile Ile Asp Asn Leu Tyr Gly Asp Asp Asn Asn Ser Ile Val Asn

210 215 220

Ser Ile Gln Gly Tyr Asp Leu Asp Asn Asp Gly Asn Ile Tyr Ile Ser

225 230 235 240

Ser Gln Lys Ala Pro Asp Phe Asp Gly Ser Tyr Tyr Ala His His Lys

245 250 255

Gln Ile Val Lys Ile Pro Tyr Tyr Ala Arg Ser Lys Glu Ser Glu Asp

260 265 270

Gln Trp Arg Ala Val Asn Leu Ser Glu Phe Gly Gly Leu Asp Ile Pro

275 280 285

Gly Lys His Ser Glu Val Glu Ser Ile Gln Ile Ile Gly Glu Asn His

290 295 300

Cys Tyr Leu Thr Val Ala Tyr His Ser Lys Asn Lys Ala Gly Glu Asn

305 310 315 320

Lys Thr Thr Leu Asn Glu Ile Tyr Glu Leu Ser Trp Asn

325 330

<210> 145

<211> 1002

<212> DNA

<213> 瑞士乳杆菌(Lactobacillus helveticus)

<400> 145

atgaagcatt taaatgaaac aactaatgtt agaattttaa gtcaatttga tatggatact 60

ggctatcaag cagtagttca aaaaggcaat gtaggttcaa aatatgtata tggattacaa 120

cttcgcaaag gtgctactac tatcttgcgt ggttaccgtg gaagtaaaat taataaccct 180

attcttgaat tatctggtca agcaggtggt cacacacaga catgggaatt tgctggtgat 240

cgtaaagaca ttaatggtga agaaagagca ggtcaatggt ttataggtgt taaaccatcg 300

aaaattgaag gaagcaaaat tatttgggca aagcaaattg caagagttga tcttagaaat 360

caaatgggac ctcattattc aaatactgac tttcctcgat tatcctactt gaatcgcgcc 420

ggttctaatc catttgctgg taataagatg acgcatgccg aagccgcagt atcacctgat 480

tatactaagt ttttaattgc tactgttgaa aataactgta ttggtcattt tactatatac 540

aatttagata caattaatga aaaacttgat gaaaagggaa atagtgaaga tgttaatctc 600

gaaactgtta aatacgaaga tagttttatc attgataatt tatatggtga tgataataat 660

tctattgtaa attcaattca agggtatgat ttggataatg atggaaatat ttatatttcc 720

agtcaaaaag cgccagattt tgatggctct tattatgcac atcataagca gattgttaag 780

attccatatt atgctcggtc taaagaaagc gaagaccaat ggagagctgt aaatttaagc 840

gaattcggtg gcttggatat tccaggtaaa catagtgaag ttgaaagcat ccaaattatt 900

ggtgagaatc attgttactt aactgttgca tatcattcta aaaataaagc gggtgaaaat 960

aaaactactt tgaatgagat ttatgaatta tcttggaatt ag 1002

<210> 146

<211> 74

<212> PRT

<213> 海氏肠球菌(Enterococcus hirae)

<400> 146

Met Lys Lys Lys Val Leu Lys His Cys Val Ile Leu Gly Ile Leu Gly

1 5 10 15

Thr Cys Leu Ala Gly Ile Gly Thr Gly Ile Lys Val Asp Ala Ala Thr

20 25 30

Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Glu Lys Cys Trp Val Asp

35 40 45

Trp Asn Gln Ala Lys Gly Glu Ile Gly Lys Ile Ile Val Asn Gly Trp

50 55 60

Val Asn His Gly Pro Trp Ala Pro Arg Arg

65 70

<210> 147

<211> 225

<212> DNA

<213> 海氏肠球菌(Enterococcus hirae)

<400> 147

atgaaaaaga aagtattaaa acattgtgtt attctaggaa tattaggaac ttgtctagct 60

ggcatcggta caggaataaa agttgatgca gctacttact atggaaatgg tctttattgt 120

aacaaagaaa aatgttgggt agattggaat caagctaaag gagaaattgg aaaaattatt 180

gttaatggtt gggttaatca tggtccatgg gcacctagaa ggtag 225

<210> 148

<211> 75

<212> PRT

<213> 约氏乳杆菌(Lactobacillus johnsonii)

<400> 148

Met Lys Gln Phe Asn Tyr Leu Ser His Lys Asp Leu Ala Val Val Val

1 5 10 15

Gly Gly Arg Asn Asn Trp Gln Thr Asn Val Gly Gly Ala Val Gly Ser

20 25 30

Ala Met Ile Gly Ala Thr Val Gly Gly Thr Ile Cys Gly Pro Ala Cys

35 40 45

Ala Val Ala Gly Ala His Tyr Leu Pro Ile Leu Trp Thr Ala Val Thr

50 55 60

Ala Ala Thr Gly Gly Phe Gly Lys Ile Arg Lys

65 70 75

<210> 149

<211> 228

<212> DNA

<213> 约氏乳杆菌(Lactobacillus johnsonii)

<400> 149

atgaaacaat ttaattattt atcacataaa gatttagcag tcgttgttgg tggaagaaat 60

aattggcaaa caaatgtggg aggagcagtg ggatcagcta tgattggggc tacagttggt 120

ggtacaattt gtggacctgc atgtgctgta gctggtgccc attatcttcc tattttatgg 180

acagcggtta cagctgcaac aggtggtttt ggcaagataa gaaagtag 228

<210> 150

<211> 62

<212> PRT

<213> 约氏乳杆菌(Lactobacillus johnsonii)

<400> 150

Met Lys Leu Asn Asp Lys Glu Leu Ser Lys Ile Val Gly Gly Asn Arg

1 5 10 15

Trp Gly Asp Thr Val Leu Ser Ala Ala Ser Gly Ala Gly Thr Gly Ile

20 25 30

Lys Ala Cys Lys Ser Phe Gly Pro Trp Gly Met Ala Ile Cys Gly Val

35 40 45

Gly Gly Ala Ala Ile Gly Gly Tyr Phe Gly Tyr Thr His Asn

50 55 60

<210> 151

<211> 189

<212> DNA

<213> 约氏乳杆菌(Lactobacillus johnsonii)

<400> 151

atgaaattaa atgacaaaga attatcaaag attgttggtg gaaatcgatg gggagatact 60

gttttatcag ctgctagtgg cgcaggaact ggtattaaag catgtaaaag ttttggccca 120

tggggaatgg caatttgtgg tgtaggaggt gcagcaatag gaggttattt tggctatact 180

cataattaa 189

<210> 152

<211> 59

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 152

Met Asn Lys Asn Glu Ile Glu Thr Gln Pro Val Thr Trp Leu Glu Glu

1 5 10 15

Val Ser Asp Gln Asn Phe Asp Glu Asp Val Phe Gly Ala Cys Ser Thr

20 25 30

Asn Thr Phe Ser Leu Ser Asp Tyr Trp Gly Asn Asn Gly Ala Trp Cys

35 40 45

Thr Leu Thr His Glu Cys Met Ala Trp Cys Lys

50 55

<210> 153

<211> 180

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 153

atgaacaaaa atgaaattga aacacaacca gttacatggt tggaagaagt atctgatcaa 60

aattttgatg aagatgtatt tggtgcgtgt agtactaaca cattctcgct cagtgattac 120

tggggaaata acggggcttg gtgtacactc actcatgaat gtatggcttg gtgtaaataa 180

<210> 154

<211> 65

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 154

Met Lys Glu Lys Asn Met Lys Lys Asn Asp Thr Ile Glu Leu Gln Leu

1 5 10 15

Gly Lys Tyr Leu Glu Asp Asp Met Ile Glu Leu Ala Glu Gly Asp Glu

20 25 30

Ser His Gly Gly Thr Thr Pro Ala Thr Pro Ala Ile Ser Ile Leu Ser

35 40 45

Ala Tyr Ile Ser Thr Asn Thr Cys Pro Thr Thr Lys Cys Thr Arg Ala

50 55 60

Cys

65

<210> 155

<211> 198

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 155

atgaaagaaa aaaatatgaa aaagaatgac actattgaat tacaattggg aaaatacctt 60

gaagatgata tgattgaatt agctgaaggg gatgagtctc atggaggaac aacaccagca 120

actcctgcaa tctctattct cagtgcatat attagtacca atacttgtcc aacaacaaaa 180

tgtacacgtg cttgttaa 198

<210> 156

<211> 51

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 156

Met Lys Glu Gln Asn Ser Phe Asn Leu Leu Gln Glu Val Thr Glu Ser

1 5 10 15

Glu Leu Asp Leu Ile Leu Gly Ala Lys Gly Gly Ser Gly Val Ile His

20 25 30

Thr Ile Ser His Glu Cys Asn Met Asn Ser Trp Gln Phe Val Phe Thr

35 40 45

Cys Cys Ser

50

<210> 157

<211> 156

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 157

atgaaagaac aaaactcttt taatcttctt caagaagtga cagaaagtga attggacctt 60

attttaggtg caaaaggcgg cagtggagtt attcatacaa tttctcatga atgtaatatg 120

aatagctggc aatttgtatt tacttgctgc tcttaa 156

<210> 158

<211> 53

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 158

Met Ala Gly Phe Leu Lys Val Val Gln Leu Leu Ala Lys Tyr Gly Ser

1 5 10 15

Lys Ala Val Gln Trp Ala Trp Ala Asn Lys Gly Lys Ile Leu Asp Trp

20 25 30

Leu Asn Ala Gly Gln Ala Ile Asp Trp Val Val Ser Lys Ile Lys Gln

35 40 45

Ile Leu Gly Ile Lys

50

<210> 159

<211> 162

<212> DNA

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 159

atggcagggt ttttaaaagt agttcaatta ctagctaaat atggttctaa agctgtacaa 60

tgggcttggg caaacaaggg taagatttta gattggctta atgcaggtca ggctattgat 120

tgggtagttt cgaaaattaa gcaaatttta ggtattaagt aa 162

<210> 160

<211> 53

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 160

Met Ala Gly Phe Leu Lys Val Val Gln Ile Leu Ala Lys Tyr Gly Ser

1 5 10 15

Lys Ala Val Gln Trp Ala Trp Ala Asn Lys Gly Lys Ile Leu Asp Trp

20 25 30

Ile Asn Ala Gly Gln Ala Ile Asp Trp Val Val Glu Lys Ile Lys Gln

35 40 45

Ile Leu Gly Ile Lys

50

<210> 161

<211> 162

<212> DNA

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 161

atggcagggt ttttaaaagt agtccaaatt ttggctaagt atggttctaa agccgtacaa 60

tgggcatggg caaataaagg aaaaatctta gattggatta atgcaggtca agctattgac 120

tgggtagttg aaaagattaa gcaaattttg ggtattaaat aa 162

<210> 162

<211> 65

<212> PRT

<213> 食淀粉乳杆菌(Lactobacillus amylovorus)

<400> 162

Met Lys Gln Leu Asn Ser Glu Gln Leu Gln Asn Ile Ile Gly Gly Asn

1 5 10 15

Arg Trp Thr Asn Ala Tyr Ser Ala Ala Leu Gly Cys Ala Val Pro Gly

20 25 30

Val Lys Tyr Gly Lys Lys Leu Gly Gly Val Trp Gly Ala Val Ile Gly

35 40 45

Gly Val Gly Gly Ala Ala Val Cys Gly Leu Ala Gly Tyr Val Arg Lys

50 55 60

Gly

65

<210> 163

<211> 198

<212> DNA

<213> 食淀粉乳杆菌(Lactobacillus amylovorus)

<400> 163

atgaaacaat tgaattcaga acaattacaa aatattatcg gtggaaatag atggactaat 60

gcatacagcg cagctttggg atgcgctgtc cctggagtta aatatggaaa aaaacttggt 120

ggcgtatggg gtgctgtaat tggtggcgta ggcggtgcag cagtctgtgg cttggcgggt 180

tatgttcgta aaggctaa 198

<210> 164

<211> 68

<212> PRT

<213> 清酒乳杆菌L45(Lactobacillus sakei L45)

<400> 164

Met Lys Thr Glu Lys Lys Val Leu Asp Glu Leu Ser Leu His Ala Ser

1 5 10 15

Ala Lys Met Gly Ala Arg Asp Val Glu Ser Ser Met Asn Ala Asp Ser

20 25 30

Thr Pro Val Leu Ala Ser Val Ala Val Ser Met Glu Leu Leu Pro Thr

35 40 45

Ala Ser Val Leu Tyr Ser Asp Val Ala Gly Cys Phe Lys Tyr Ser Ala

50 55 60

Lys His His Cys

65

<210> 165

<211> 207

<212> DNA

<213> 清酒乳杆菌L45(Lactobacillus sakei L45)

<400> 165

atgaaaacag aaaaaaaggt tttagatgaa ctgagcttac acgcttctgc aaaaatggga 60

gcacgtgatg ttgaatccag catgaatgca gactcaacac cagttttagc atcagtcgct 120

gtatccatgg aattattgcc aactgcgtct gttctttatt cggatgttgc aggttgcttc 180

aaatattctg caaaacatca ttgttag 207

<210> 166

<211> 91

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 166

Met Lys Thr Lys Ser Leu Val Leu Ala Leu Ser Ala Val Thr Leu Phe

1 5 10 15

Ser Ala Gly Gly Ile Val Ala Gln Ala Glu Gly Thr Trp Gln His Gly

20 25 30

Tyr Gly Val Ser Ser Ala Tyr Ser Asn Tyr His His Gly Ser Lys Thr

35 40 45

His Ser Ala Thr Val Val Asn Asn Asn Thr Gly Arg Gln Gly Lys Asp

50 55 60

Thr Gln Arg Ala Gly Val Trp Ala Lys Ala Thr Val Gly Arg Asn Leu

65 70 75 80

Thr Glu Lys Ala Ser Phe Tyr Tyr Asn Phe Trp

85 90

<210> 167

<211> 276

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 167

atgaaaacca agtctctcgt attggcatta tctgcggtta cgttattctc tgccggagga 60

attgtagctc aagctgaagg aacatggcaa catggatatg gtgttagttc ggcatattca 120

aattatcatc atggtagcaa aactcattca gccacagttg taaataataa tactggccga 180

caaggtaagg atacacaacg tgccggtgtt tgggcaaaag ctactgttgg acgtaactta 240

actgaaaaag cttcatttta ttataacttt tggtaa 276

<210> 168

<211> 75

<212> PRT

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 168

Met Lys Asn Gln Leu Asn Phe Asn Ile Val Ser Asp Glu Glu Leu Ser

1 5 10 15

Glu Ala Asn Gly Gly Lys Leu Thr Phe Ile Gln Ser Thr Ala Ala Gly

20 25 30

Asp Leu Tyr Tyr Asn Thr Asn Thr His Lys Tyr Val Tyr Gln Gln Thr

35 40 45

Gln Asn Ala Phe Gly Ala Ala Ala Asn Thr Ile Val Asn Gly Trp Met

50 55 60

Gly Gly Ala Ala Gly Gly Phe Gly Leu His His

65 70 75

<210> 169

<211> 228

<212> DNA

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 169

atgaaaaatc aattaaattt taatattgtt tcagatgaag aactttcaga agctaacgga 60

ggaaaattaa catttattca atcgacagcg gctggagatt tatattacaa tactaataca 120

cacaaatatg tttaccaaca aactcaaaac gcttttgggg ctgctgctaa taccattgtt 180

aatggatgga tgggtggcgc tgctggaggt ttcgggttgc accattga 228

<210> 170

<211> 68

<212> PRT

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 170

Met Lys Asn Gln Leu Asn Phe Asn Ile Val Ser Asp Glu Glu Leu Ala

1 5 10 15

Glu Val Asn Gly Gly Ser Leu Gln Tyr Val Met Ser Ala Gly Pro Tyr

20 25 30

Thr Trp Tyr Lys Asp Thr Arg Thr Gly Lys Thr Ile Cys Lys Gln Thr

35 40 45

Ile Asp Thr Ala Ser Tyr Thr Phe Gly Val Met Ala Glu Gly Trp Gly

50 55 60

Lys Thr Phe His

65

<210> 171

<211> 207

<212> DNA

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 171

atgaaaaatc aattaaattt taatattgtt tctgatgaag aacttgcaga agttaatgga 60

ggaagcttgc agtatgttat gagtgctgga ccatatactt ggtataaaga tactagaaca 120

ggaaaaacaa tatgtaaaca gacaattgac acagcaagtt atacatttgg tgtaatggca 180

gaaggatggg gaaaaacatt ccactaa 207

<210> 172

<211> 63

<212> PRT

<213> 乳球菌QU 12(Lactococcus sp. QU 12)

<400> 172

Met Lys Leu Ile Asp His Leu Gly Ala Pro Arg Trp Ala Val Asp Thr

1 5 10 15

Ile Leu Gly Ala Ile Ala Val Gly Asn Leu Ala Ser Trp Val Leu Ala

20 25 30

Leu Val Pro Gly Pro Gly Trp Ala Val Lys Ala Gly Leu Ala Thr Ala

35 40 45

Ala Ala Ile Val Lys His Gln Gly Lys Ala Ala Ala Ala Ala Trp

50 55 60

<210> 173

<211> 192

<212> DNA

<213> 乳球菌QU 12(Lactococcus sp. QU 12)

<400> 173

atgaaattaa ttgatcattt aggtgctcca agatgggccg ttgatactat tttaggtgca 60

atcgcagttg ggaacttagc aagttgggtt ctagcgcttg tccctggtcc agggtgggca 120

gtaaaagctg gtttagcaac tgctgctgcc atcgttaaac atcaaggtaa agctgccgct 180

gctgcttggt aa 192

<210> 174

<211> 50

<212> PRT

<213> 芽孢杆菌GI-9(Brevibacillus sp. GI-9)

<400> 174

Met Ala Cys Gln Cys Pro Asp Ala Ile Ser Gly Trp Thr His Thr Asp

1 5 10 15

Tyr Gln Cys His Gly Leu Glu Asn Lys Met Tyr Arg His Val Tyr Ala

20 25 30

Ile Cys Met Asn Gly Thr Gln Val Tyr Cys Arg Thr Glu Trp Gly Ser

35 40 45

Ser Cys

50

<210> 175

<211> 153

<212> DNA

<213> 芽孢杆菌GI-9(Brevibacillus sp. GI-9)

<400> 175

atggcttgcc aatgtccaga tgcgatctca ggttggacgc atacagatta ccagtgtcac 60

ggtttggaga ataaaatgta tagacatgtt tatgcaattt gcatgaacgg tactcaagta 120

tattgcagaa cagagtgggg tagcagctgc tag 153

<210> 176

<211> 57

<212> PRT

<213> 假肠膜明串珠菌(Leuconostoc pseudomesenteroides)

<400> 176

Met Asn Lys Glu Tyr Asn Ser Ile Ser Asn Phe Lys Lys Ile Thr Asn

1 5 10 15

Lys Asp Leu Gln Asn Ile Asn Gly Gly Phe Ile Gly Arg Ala Ile Gly

20 25 30

Asp Phe Val Tyr Phe Gly Ala Lys Gly Leu Arg Glu Ser Gly Lys Leu

35 40 45

Leu Asn Tyr Tyr Tyr Lys His Lys His

50 55

<210> 177

<211> 174

<212> DNA

<213> 假肠膜明串珠菌(Leuconostoc pseudomesenteroides)

<400> 177

atgaataaag aatataatag cattagcaat tttaaaaaaa ttactaataa agacttgcaa 60

aacataaatg gtggatttat tggtagggca ataggtgact ttgtgtactt tggagcgaag 120

ggactaagag aatctggtaa actacttaat tattactata agcataagca ttga 174

<210> 178

<211> 53

<212> PRT

<213> 假肠膜明串珠菌(Leuconostoc pseudomesenteroides)

<400> 178

Met Lys Asn Gln Leu Met Ser Phe Glu Val Ile Ser Glu Lys Glu Leu

1 5 10 15

Ser Thr Val Gln Gly Gly Lys Gly Leu Gly Lys Leu Ile Gly Ile Asp

20 25 30

Trp Leu Leu Gly Gln Ala Lys Asp Ala Val Lys Gln Tyr Lys Lys Asp

35 40 45

Tyr Lys Arg Trp His

50

<210> 179

<211> 162

<212> DNA

<213> 假肠膜明串珠菌(Leuconostoc pseudomesenteroides)

<400> 179

atgaaaaatc agttaatgtc tttcgaagtg atatcagaaa aagaattgtc cacggtacaa 60

ggtggcaaag gcttaggtaa actcatagga attgattggc ttttgggtca agctaaggac 120

gctgttaaac agtacaagaa ggattacaaa cgttggcact aa 162

<210> 180

<211> 61

<212> PRT

<213> 冷生明串珠菌(Leuconostoc gelidum)

<400> 180

Met Met Asn Met Lys Pro Thr Glu Ser Tyr Glu Gln Leu Asp Asn Ser

1 5 10 15

Ala Leu Glu Gln Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val His

20 25 30

Cys Thr Lys Ser Gly Cys Ser Val Asn Trp Gly Glu Ala Phe Ser Ala

35 40 45

Gly Val His Arg Leu Ala Asn Gly Gly Asn Gly Phe Trp

50 55 60

<210> 181

<211> 186

<212> DNA

<213> 冷生明串珠菌(Leuconostoc gelidum)

<400> 181

atgatgaaca tgaaacctac ggaaagctat gagcaattgg ataatagtgc tctcgaacaa 60

gtcgtaggag gtaagtatta tggtaacgga gttcattgca caaaaagtgg ttgttctgta 120

aactggggag aagccttttc agctggagta catcgtttag caaatggtgg aaatggtttc 180

tggtaa 186

<210> 182

<211> 61

<212> PRT

<213> 肉质明串珠菌(Leuconostoc carnosum)

<400> 182

Met Asn Asn Met Lys Ser Ala Asp Asn Tyr Gln Gln Leu Asp Asn Asn

1 5 10 15

Ala Leu Glu Gln Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val His

20 25 30

Cys Thr Lys Ser Gly Cys Ser Val Asn Trp Gly Glu Ala Phe Ser Ala

35 40 45

Gly Val His Arg Leu Ala Asn Gly Gly Asn Gly Phe Trp

50 55 60

<210> 183

<211> 186

<212> DNA

<213> 肉质明串珠菌(Leuconostoc carnosum)

<400> 183

atgaataaca tgaaatctgc ggataattat cagcaattgg ataataatgc tctcgaacaa 60

gtcgtaggag gtaagtatta tggtaacgga gttcattgca caaaaagtgg ttgttctgta 120

aactggggag aagccttttc agctggagta catcgtttag caaatggtgg aaatggtttc 180

tggtaa 186

<210> 184

<211> 63

<212> PRT

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 184

Met Phe Leu Val Asn Gln Leu Gly Ile Ser Lys Ser Leu Ala Asn Thr

1 5 10 15

Ile Leu Gly Ala Ile Ala Val Gly Asn Leu Ala Ser Trp Leu Leu Ala

20 25 30

Leu Val Pro Gly Pro Gly Trp Ala Thr Lys Ala Ala Leu Ala Thr Ala

35 40 45

Glu Thr Ile Val Lys His Glu Gly Lys Ala Ala Ala Ile Ala Trp

50 55 60

<210> 185

<211> 192

<212> DNA

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 185

atgttcttgg taaatcagtt agggatttca aaatcgttag ctaatactat tcttggtgca 60

attgctgttg gtaatttggc cagttggtta ttagctttgg ttcctggtcc gggttgggca 120

acaaaagcag cacttgcgac agctgaaaca attgtgaagc atgaaggaaa agcagctgct 180

attgcgtggt aa 192

<210> 186

<211> 74

<212> PRT

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<400> 186

Met Ser Lys Lys Glu Met Ile Leu Ser Trp Lys Asn Pro Met Tyr Arg

1 5 10 15

Thr Glu Ser Ser Tyr His Pro Ala Gly Asn Ile Leu Lys Glu Leu Gln

20 25 30

Glu Glu Glu Gln His Ser Ile Ala Gly Gly Thr Ile Thr Leu Ser Thr

35 40 45

Cys Ala Ile Leu Ser Lys Pro Leu Gly Asn Asn Gly Tyr Leu Cys Thr

50 55 60

Val Thr Lys Glu Cys Met Pro Ser Cys Asn

65 70

<210> 187

<211> 225

<212> DNA

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<400> 187

atgtcaaaaa aggaaatgat tctttcatgg aaaaatccta tgtatcgcac tgaatcttct 60

tatcatccag cagggaacat ccttaaagaa ctccaggaag aggaacagca cagcatcgcc 120

ggaggcacaa tcacgctcag cacttgtgcc atcttgagca agccgttagg aaataacgga 180

tacctgtgta cagtgacaaa agaatgcatg ccaagctgta actaa 225

<210> 188

<211> 266

<212> PRT

<213> 亚麻短杆菌(Brevibacterium linens)

<400> 188

Met Asn Asn Leu Tyr Arg Glu Leu Ala Pro Ile Pro Gly Pro Ala Trp

1 5 10 15

Ala Glu Ile Glu Glu Glu Ala Arg Arg Thr Phe Lys Arg Asn Ile Ala

20 25 30

Gly Arg Arg Ile Val Asp Val Ala Gly Pro Thr Gly Phe Glu Thr Ser

35 40 45

Ala Val Thr Thr Gly His Ile Arg Asp Val Gln Ser Glu Thr Ser Gly

50 55 60

Leu Gln Val Lys Gln Arg Ile Val Gln Glu Tyr Ile Glu Leu Arg Thr

65 70 75 80

Pro Phe Thr Val Thr Arg Gln Ala Ile Asp Asp Val Ala Arg Gly Ser

85 90 95

Gly Asp Ser Asp Trp Gln Pro Val Lys Asp Ala Ala Thr Thr Ile Ala

100 105 110

Met Ala Glu Asp Arg Ala Ile Leu His Gly Leu Asp Ala Ala Gly Ile

115 120 125

Gly Gly Ile Val Pro Gly Ser Ser Asn Ala Ala Val Ala Ile Pro Asp

130 135 140

Ala Val Glu Asp Phe Ala Asp Ala Val Ala Gln Ala Leu Ser Val Leu

145 150 155 160

Arg Thr Val Gly Val Asp Gly Pro Tyr Ser Leu Leu Leu Ser Ser Ala

165 170 175

Glu Tyr Thr Lys Val Ser Glu Ser Thr Asp His Gly Tyr Pro Ile Arg

180 185 190

Glu His Leu Ser Arg Gln Leu Gly Ala Gly Glu Ile Ile Trp Ala Pro

195 200 205

Ala Leu Glu Gly Ala Leu Leu Val Ser Thr Arg Gly Gly Asp Tyr Glu

210 215 220

Leu His Leu Gly Gln Asp Leu Ser Ile Gly Tyr Tyr Ser His Asp Ser

225 230 235 240

Glu Thr Val Glu Leu Tyr Leu Gln Glu Thr Phe Gly Phe Leu Ala Leu

245 250 255

Thr Asp Glu Ser Ser Val Pro Leu Ser Leu

260 265

<210> 189

<211> 801

<212> DNA

<213> 亚麻短杆菌(Brevibacterium linens)

<400> 189

gtgaataacc tctatcgcga gcttgccccc atccccggcc cggcctgggc ggagatcgag 60

gaggaggctc gacggacatt caaacgcaat atcgccggcc gccggatcgt cgatgtcgca 120

gggcccacgg gcttcgagac ctccgcggtg accactggcc acatccgaga cgtccagtcg 180

gagacgagcg gactgcaggt taagcagcgc atcgtgcagg aatacatcga gctgcggacc 240

ccattcaccg tgactcggca ggccatcgat gacgtggccc gcgggtccgg tgactcggac 300

tggcagcccg tcaaggatgc ggccacgacg atcgcgatgg ctgaagatcg ggccattctc 360

cacgggctcg atgcggccgg gatcggcgga atcgttcccg gcagctcgaa tgccgcagtg 420

gccatccccg acgccgtcga ggacttcgcg gacgccgtcg cccaggcgct gagtgtgctg 480

cgcacggtgg gagtcgacgg gccctacagc ctgttgctct cctccgcgga gtacaccaag 540

gtctccgagt ccaccgacca cggctacccg atccgcgagc acctctcccg gcagctcggc 600

gccggagaga tcatctgggc gcccgcgctc gaaggggcgc tgctcgtctc cacgcgcggg 660

ggtgactacg agctccacct cggccaggac ctgtcgatcg gttactacag ccacgacagc 720

gagaccgtcg aactctatct gcaggagacc ttcggattcc tcgcgctgac cgacgaatcc 780

agtgtgcctt tgagcctctg a 801

<210> 190

<211> 71

<212> PRT

<213> 无害利斯特菌(Listeria innocua)

<400> 190

Met Lys Lys Ala Ala Leu Lys Phe Ile Ile Val Ile Ala Ile Leu Gly

1 5 10 15

Phe Ser Phe Ser Phe Phe Ser Ile Gln Ser Glu Ala Lys Ser Tyr Gly

20 25 30

Asn Gly Val Gln Cys Asn Lys Lys Lys Cys Trp Val Asp Trp Gly Ser

35 40 45

Ala Ile Ser Thr Ile Gly Asn Asn Ser Ala Ala Asn Trp Ala Thr Gly

50 55 60

Gly Ala Ala Gly Trp Lys Ser

65 70

<210> 191

<211> 216

<212> DNA

<213> 无害利斯特菌(Listeria innocua)

<400> 191

ttgaagaagg cagcgttaaa atttattatt gttattgcta ttctaggttt cagtttttct 60

ttctttagca tacaatctga agctaaatct tatggaaatg gagttcagtg taataagaaa 120

aaatgttggg tagattgggg tagtgctata agtactattg gaaataattc tgcagcgaat 180

tgggctacag gtggagcagc tggttggaaa agctga 216

<210> 192

<211> 68

<212> PRT

<213> 芽孢杆菌(Bacillus sp.)

<400> 192

Met Ser Gln Glu Ala Ile Ile Arg Ser Trp Lys Asp Pro Phe Ser Arg

1 5 10 15

Glu Asn Ser Thr Gln Asn Pro Ala Gly Asn Pro Phe Ser Glu Leu Lys

20 25 30

Glu Ala Gln Met Asp Lys Leu Val Gly Ala Gly Asp Met Glu Ala Ala

35 40 45

Cys Thr Phe Thr Leu Pro Gly Gly Gly Gly Val Cys Thr Leu Thr Ser

50 55 60

Glu Cys Ile Cys

65

<210> 193

<211> 207

<212> DNA

<213> 芽孢杆菌(Bacillus sp.)

<400> 193

atgagtcaag aagctatcat tcgttcatgg aaagatcctt tttcccgtga aaattctaca 60

caaaatccag ctggtaaccc attcagtgag ctgaaagaag cacaaatgga taagttagta 120

ggtgcgggag acatggaagc agcatgtact tttacattgc ctggtggcgg cggtgtttgt 180

actctaactt ctgaatgtat ttgttaa 207

<210> 194

<211> 61

<212> PRT

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 194

Met Thr Asn Met Lys Ser Val Glu Ala Tyr Gln Gln Leu Asp Asn Gln

1 5 10 15

Asn Leu Lys Lys Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val His

20 25 30

Cys Thr Lys Ser Gly Cys Ser Val Asn Trp Gly Glu Ala Ala Ser Ala

35 40 45

Gly Ile His Arg Leu Ala Asn Gly Gly Asn Gly Phe Trp

50 55 60

<210> 195

<211> 186

<212> DNA

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 195

atgacgaata tgaagtctgt ggaagcatat cagcaattag ataaccagaa tctcaagaaa 60

gttgttggtg gaaagtatta tgggaatggt gttcactgta caaaaagtgg atgctctgtt 120

aactggggag aagctgcctc agctggcata catcgtttgg ccaatggtgg aaatggattt 180

tggtaa 186

<210> 196

<211> 68

<212> PRT

<213> 番茄细菌性溃疡病菌亚种(Clavibacter michiganensis subsp.Michiganensis)

<400> 196

Met Asn Asp Ile Leu Glu Thr Glu Thr Pro Val Met Val Ser Pro Arg

1 5 10 15

Trp Asp Met Leu Leu Asp Ala Gly Glu Asp Thr Ser Pro Ser Val Gln

20 25 30

Thr Gln Ile Asp Ala Glu Phe Arg Arg Val Val Ser Pro Tyr Met Ser

35 40 45

Ser Ser Gly Trp Leu Cys Thr Leu Thr Ile Glu Cys Gly Thr Ile Ile

50 55 60

Cys Ala Cys Arg

65

<210> 197

<211> 207

<212> DNA

<213> 番茄细菌性溃疡病菌亚种(Clavibacter michiganensis subsp.Michiganensis)

<400> 197

atgaacgaca tcctcgagac ggagaccccc gtcatggtca gcccccggtg ggacatgctg 60

ctcgacgcgg gcgaggacac cagcccgtcc gtccagaccc agatcgacgc ggagttccgt 120

cgcgtcgtga gcccgtacat gtccagcagc ggctggctct gcacgctcac catcgaatgt 180

ggcaccatca tctgcgcgtg tcgctga 207

<210> 198

<211> 69

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 198

Met Glu Leu Lys Ala Ser Glu Phe Gly Val Val Leu Ser Val Asp Ala

1 5 10 15

Leu Lys Leu Ser Arg Gln Ser Pro Leu Gly Val Gly Ile Gly Gly Gly

20 25 30

Gly Gly Gly Gly Gly Gly Gly Ser Cys Gly Gly Gln Gly Gly Gly Cys

35 40 45

Gly Gly Cys Ser Asn Gly Cys Ser Gly Gly Asn Gly Gly Ser Gly Gly

50 55 60

Ser Gly Ser His Ile

65

<210> 199

<211> 207

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 199

atggaattaa aagcgagtga atttggtgta gttttgtccg ttgatgctct taaattatca 60

cgccagtctc cattaggtgt tggcattggt ggtggtggcg gcggcggcgg cggcggtagc 120

tgcggtggtc aaggtggcgg ttgtggtggt tgcagcaacg gttgtagtgg tggaaacggt 180

ggcagcggcg gaagtggttc acatatc 207

<210> 200

<211> 7

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 200

Met Arg Thr Gly Asn Ala Asn

1 5

<210> 201

<211> 24

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 201

atgcgtactg gtaatgcaaa ctaa 24

<210> 202

<211> 99

<212> PRT

<213> 肺炎克雷伯菌(Klebsiella pneumoniae)

<400> 202

Met Arg Glu Ile Ser Gln Lys Asp Leu Asn Leu Ala Phe Gly Ala Gly

1 5 10 15

Glu Thr Asp Pro Asn Thr Gln Leu Leu Asn Asp Leu Gly Asn Asn Met

20 25 30

Ala Trp Gly Ala Ala Leu Gly Ala Pro Gly Gly Leu Gly Ser Ala Ala

35 40 45

Leu Gly Ala Ala Gly Gly Ala Leu Gln Thr Val Gly Gln Gly Leu Ile

50 55 60

Asp His Gly Pro Val Asn Val Pro Ile Pro Val Leu Ile Gly Pro Ser

65 70 75 80

Trp Asn Gly Ser Gly Ser Gly Tyr Asn Ser Ala Thr Ser Ser Ser Gly

85 90 95

Ser Gly Ser

<210> 203

<211> 300

<212> DNA

<213> 肺炎克雷伯菌(Klebsiella pneumoniae)

<400> 203

atgagagaaa ttagtcaaaa ggacttaaat cttgcttttg gtgcaggaga gaccgatcca 60

aatactcaac ttctaaacga ccttggaaat aatatggcat ggggtgctgc tcttggcgct 120

cctggcggat taggatcagc agctttgggg gccgcgggag gtgcattaca aactgtaggg 180

caaggattaa ttgaccatgg tcctgtaaat gtccccatcc ctgtactcat cgggccaagc 240

tggaatggta gcggtagtgg ttataacagc gcaacatcca gttccggtag tggtagttaa 300

<210> 204

<211> 75

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 204

Met Arg Glu Ile Thr Glu Ser Gln Leu Arg Tyr Ile Ser Gly Ala Gly

1 5 10 15

Gly Ala Pro Ala Thr Ser Ala Asn Ala Ala Gly Ala Ala Ala Ile Val

20 25 30

Gly Ala Leu Ala Gly Ile Pro Gly Gly Pro Leu Gly Val Val Val Gly

35 40 45

Ala Val Ser Ala Gly Leu Thr Thr Ala Ile Gly Ser Thr Val Gly Ser

50 55 60

Gly Ser Ala Ser Ser Ser Ala Gly Gly Gly Ser

65 70 75

<210> 205

<211> 228

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 205

atgcgagaaa taacagaatc acagttaaga tatatttccg gggcgggagg tgcgccagcg 60

acttcagcta atgccgcagg tgctgcagct attgttggag ctctcgccgg aatacctggt 120

ggtccacttg gggttgtagt tggagccgta tctgccggtt tgacaacagc aattggctcg 180

accgtgggaa gtggtagtgc cagttcttct gctggtggcg gtagctaa 228

<210> 206

<211> 58

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 206

Met Ile Lys His Phe His Phe Asn Lys Leu Ser Ser Gly Lys Lys Asn

1 5 10 15

Asn Val Pro Ser Pro Ala Lys Gly Val Ile Gln Ile Lys Lys Ser Ala

20 25 30

Ser Gln Leu Thr Lys Gly Gly Ala Gly His Val Pro Glu Tyr Phe Val

35 40 45

Gly Ile Gly Thr Pro Ile Ser Phe Tyr Gly

50 55

<210> 207

<211> 177

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 207

atgattaagc attttcattt taataaactg tcttctggta aaaaaaataa tgttccatct 60

cctgcaaagg gggttataca aataaaaaaa tcagcatcgc aactcacaaa aggtggtgca 120

ggacatgtgc ctgagtattt tgtggggatt ggtacaccta tatctttcta tggctga 177

<210> 208

<211> 90

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 208

Met Tyr Met Arg Glu Leu Asp Arg Glu Glu Leu Asn Cys Val Gly Gly

1 5 10 15

Ala Gly Asp Pro Leu Ala Asp Pro Asn Ser Gln Ile Val Arg Gln Ile

20 25 30

Met Ser Asn Ala Ala Trp Gly Pro Pro Leu Val Pro Glu Arg Phe Arg

35 40 45

Gly Met Ala Val Gly Ala Ala Gly Gly Val Thr Gln Thr Val Leu Gln

50 55 60

Gly Ala Ala Ala His Met Pro Val Asn Val Pro Ile Pro Lys Val Pro

65 70 75 80

Met Gly Pro Ser Trp Asn Gly Ser Lys Gly

85 90

<210> 209

<211> 273

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 209

atgtatatga gagagttaga tagagaggaa ttaaattgcg ttggtggggc tggagatccg 60

cttgcagatc ctaattccca aattgtaaga cagataatgt ctaatgcggc atggggcccg 120

cctttggtgc cagagcggtt taggggaatg gctgttggag ccgcaggtgg ggttacgcag 180

acagttcttc aaggagcagc agctcatatg ccggttaatg tccctatacc taaagttccg 240

atgggaccct catggaacgg aagtaaagga taa 273

<210> 210

<211> 50

<212> PRT

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 210

Met Ser Gln Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys

1 5 10 15

Asn Lys Lys Gly Cys Ser Val Asp Trp Gly Lys Ala Ile Gly Ile Ile

20 25 30

Gly Asn Asn Ser Ala Ala Asn Leu Ala Thr Gly Gly Ala Ala Gly Trp

35 40 45

Lys Ser

50

<210> 211

<211> 153

<212> DNA

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 211

atgtcacagg tagtaggtgg aaaatactac ggtaatggag tctcatgtaa taaaaaaggg 60

tgcagtgttg attggggaaa agcgattggc attattggaa ataattctgc tgcgaattta 120

gctactggtg gagcagctgg ttggaaaagt taa 153

<210> 212

<211> 58

<212> PRT

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 212

Met Lys Lys Leu Thr Ser Lys Glu Met Ala Gln Val Val Gly Gly Lys

1 5 10 15

Tyr Tyr Gly Asn Gly Leu Ser Cys Asn Lys Lys Gly Cys Ser Val Asp

20 25 30

Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala Asn Leu

35 40 45

Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser

50 55

<210> 213

<211> 177

<212> DNA

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 213

ttgaagaaat taacatcaaa agaaatggca caagtagtag gtgggaaata ctacggtaat 60

ggattatcat gtaataaaaa agggtgcagt gttgattggg gaaaagctat tggcattatt 120

ggaaataatt ctgctgcgaa tttagctact ggtggagcag ctggttggaa aagttaa 177

<210> 214

<211> 63

<212> PRT

<213> 变异链球菌(Streptococcus mutans)

<400> 214

Met Ser Asn Thr Gln Leu Leu Glu Val Leu Gly Thr Glu Thr Phe Asp

1 5 10 15

Val Gln Glu Asp Leu Phe Ala Phe Asp Thr Thr Asp Thr Thr Ile Val

20 25 30

Ala Ser Asn Asp Asp Pro Asp Thr Arg Phe Lys Ser Trp Ser Leu Cys

35 40 45

Thr Pro Gly Cys Ala Arg Thr Gly Ser Phe Asn Ser Tyr Cys Cys

50 55 60

<210> 215

<211> 192

<212> DNA

<213> 变异链球菌(Streptococcus mutans)

<400> 215

atgtcaaaca cacaattatt agaagtcctt ggtactgaaa cttttgatgt tcaagaagat 60

ctctttgctt ttgatacaac agatactact attgtggcaa gcaacgacga tccagatact 120

cgtttcaaaa gttggagcct ttgtacgcct ggttgtgcaa ggacaggtag tttcaatagt 180

tactgttgct ga 192

<210> 216

<211> 53

<212> PRT

<213> 变异链球菌(Streptococcus mutans)

<400> 216

Met Asn Lys Leu Asn Ser Asn Ala Val Val Ser Leu Asn Glu Val Ser

1 5 10 15

Asp Ser Glu Leu Asp Thr Ile Leu Gly Gly Asn Arg Trp Trp Gln Gly

20 25 30

Val Val Pro Thr Val Ser Tyr Glu Cys Arg Met Asn Ser Trp Gln His

35 40 45

Val Phe Thr Cys Cys

50

<210> 217

<211> 162

<212> DNA

<213> 变异链球菌(Streptococcus mutans)

<400> 217

atgaacaagt taaacagtaa cgcagtagtt tctttgaatg aagtttcaga ttctgaattg 60

gatactattt tgggtggtaa tcgttggtgg caaggtgttg tgccaacggt ctcatatgag 120

tgtcgcatga attcatggca acatgttttc acttgctgtt aa 162

<210> 218

<211> 57

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 218

Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys

1 5 10 15

Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro

20 25 30

Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr

35 40 45

Cys His Cys Ser Ile His Val Ser Lys

50 55

<210> 219

<211> 174

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 219

atgagtacaa aagattttaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca 60

tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg 120

ggttgtaaca tgaaaacagc aacttgtcat tgtagtattc acgtaagcaa ataa 174

<210> 220

<211> 57

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 220

Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys

1 5 10 15

Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro

20 25 30

Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr

35 40 45

Cys Asn Cys Ser Val His Val Ser Lys

50 55

<210> 221

<211> 171

<212> DNA

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 221

atgagtacaa aagatttcaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca 60

tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg 120

ggttgtaaca tgaaaacagc aacttgtaat tgtagcgttc acgtaagcaa a 171

<210> 222

<211> 57

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 222

Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Thr

1 5 10 15

Asp Ser Gly Ala Ser Thr Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro

20 25 30

Gly Cys Lys Thr Gly Val Leu Met Gly Cys Asn Leu Lys Thr Ala Thr

35 40 45

Cys Asn Cys Ser Val His Val Ser Lys

50 55

<210> 223

<211> 174

<212> DNA

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 223

atgagtacaa aagatttcaa cttagatttg gtatctgttt caaaaacaga ttctggcgct 60

tcaacacgta ttaccagcat ttcgctttgt acaccaggtt gtaaaacagg tgttctgatg 120

ggatgtaacc tgaaaacagc aacttgtaat tgtagcgttc acgtaagcaa ataa 174

<210> 224

<211> 55

<212> PRT

<213> 乳房链球菌(Streptococcus uberis)

<400> 224

Met Asn Asn Glu Asp Phe Asn Leu Asp Leu Ile Lys Ile Ser Lys Glu

1 5 10 15

Asn Asn Ser Gly Ala Ser Pro Arg Ile Thr Ser Lys Ser Leu Cys Thr

20 25 30

Pro Gly Cys Lys Thr Gly Ile Leu Met Thr Cys Pro Leu Lys Thr Ala

35 40 45

Thr Cys Gly Cys His Phe Gly

50 55

<210> 225

<211> 168

<212> DNA

<213> 乳房链球菌(Streptococcus uberis)

<400> 225

atgaacaatg aagattttaa tttggatctc atcaaaatct caaaggaaaa caactcagga 60

gcttcacctc gaataactag taaatcatta tgtactcctg gatgtaagac gggtattttg 120

atgacttgtc cactaaaaac tgcaacctgt ggttgtcatt ttggataa 168

<210> 226

<211> 57

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 226

Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys

1 5 10 15

Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro

20 25 30

Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr

35 40 45

Cys Asn Cys Ser Ile His Val Ser Lys

50 55

<210> 227

<211> 174

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 227

atgagtacaa aagattttaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca 60

tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg 120

ggttgtaaca tgaaaacagc aacttgtaat tgtagtattc acgtaagcaa ataa 174

<210> 228

<211> 57

<212> PRT

<213> 沃氏葡萄球菌(Staphylococcus warneri)

<400> 228

Met Glu Asn Ser Lys Val Met Lys Asp Ile Glu Val Ala Asn Leu Leu

1 5 10 15

Glu Glu Val Gln Glu Asp Glu Leu Asn Glu Val Leu Gly Ala Lys Lys

20 25 30

Lys Ser Gly Val Ile Pro Thr Val Ser His Asp Cys His Met Asn Ser

35 40 45

Phe Gln Phe Val Phe Thr Cys Cys Ser

50 55

<210> 229

<211> 174

<212> DNA

<213> 沃氏葡萄球菌(Staphylococcus warneri)

<400> 229

atggaaaatt ctaaagttat gaaggacatt gaagtagcaa atttattaga agaggttcaa 60

gaagatgaat tgaatgaagt cttaggagct aagaaaaagt caggagtaat cccaactgtg 120

tcacacgatt gccatatgaa ttctttccaa tttgtattta cttgttgttc ataa 174

<210> 230

<211> 58

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 230

Met Ala Glu Asn Leu Phe Asp Leu Asp Ile Gln Val Asn Lys Ser Gln

1 5 10 15

Gly Ser Val Glu Pro Gln Val Leu Ser Ile Val Ala Cys Ser Ser Gly

20 25 30

Cys Gly Ser Gly Lys Thr Ala Ala Ser Cys Val Glu Thr Cys Gly Asn

35 40 45

Arg Cys Phe Thr Asn Val Gly Ser Leu Cys

50 55

<210> 231

<211> 177

<212> DNA

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 231

atggctgaaa acttatttga tctggacatt caagtaaaca aatctcaagg ttctgtagag 60

cctcaggttc tgagcattgt tgcatgttct agcggatgtg gtagcggtaa aacagctgcc 120

agttgtgttg aaacttgtgg caaccggtgc tttactaacg ttggttcact ctgctaa 177

<210> 232

<211> 62

<212> PRT

<213> 嗜酸小球菌(Pediococcus acidilactici)

<400> 232

Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile Ile

1 5 10 15

Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser Cys

20 25 30

Ser Val Asp Trp Gly Lys Ala Thr Thr Cys Ile Ile Asn Asn Gly Ala

35 40 45

Met Ala Trp Ala Thr Gly Gly His Gln Gly Asn His Lys Cys

50 55 60

<210> 233

<211> 189

<212> DNA

<213> 嗜酸小球菌(Pediococcus acidilactici)

<400> 233

atgaaaaaaa ttgaaaaatt aactgaaaaa gaaatggcca atatcattgg tggtaaatac 60

tacggtaatg gggttacttg tggcaaacat tcctgctctg ttgactgggg taaggctacc 120

acttgcataa tcaataatgg agctatggca tgggctactg gtggacatca aggtaatcat 180

aaatgctag 189

<210> 234

<211> 60

<212> PRT

<213> 戊糖片球菌(Pediococcus pentosaceus)

<400> 234

Met Thr Glu Ile Lys Val Leu Asn Asp Lys Glu Leu Lys Asn Val Val

1 5 10 15

Gly Gly Lys Tyr Tyr Gly Asn Gly Val His Cys Gly Lys Lys Thr Cys

20 25 30

Tyr Val Asp Trp Gly Gln Ala Thr Ala Ser Ile Gly Lys Ile Ile Val

35 40 45

Asn Gly Trp Thr Gln His Gly Pro Trp Ala His Arg

50 55 60

<210> 235

<211> 183

<212> DNA

<213> 戊糖片球菌(Pediococcus pentosaceus)

<400> 235

atgactgaaa ttaaagtact aaacgataag gaactaaaaa atgtcgtagg aggaaagtat 60

tacggtaacg gagtgcattg tggtaaaaag acttgctatg tggactgggg acaagctaca 120

gctagcattg gaaaaattat agtgaacgga tggacacaac acgggccttg ggcacataga 180

taa 183

<210> 236

<211> 60

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 236

Met Lys Asn Asn Lys Asn Leu Phe Asp Leu Glu Ile Lys Lys Glu Thr

1 5 10 15

Ser Gln Asn Thr Asp Glu Leu Glu Pro Gln Thr Ala Gly Pro Ala Ile

20 25 30

Arg Ala Ser Val Lys Gln Cys Gln Lys Thr Leu Lys Ala Thr Arg Leu

35 40 45

Phe Thr Val Ser Cys Lys Gly Lys Asn Gly Cys Lys

50 55 60

<210> 237

<211> 183

<212> DNA

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 237

atgaaaaata acaaaaattt atttgattta gaaattaaaa aagaaacaag tcaaaacact 60

gatgaacttg aacctcaaac tgctggacca gcgattagag cttctgtgaa acaatgtcag 120

aaaactttga aagctacgcg tttatttaca gtgtcttgca aaggaaaaaa cggatgtaaa 180

tag 183

<210> 238

<211> 62

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 238

Met Lys Thr Val Lys Glu Leu Ser Val Lys Glu Met Gln Leu Thr Thr

1 5 10 15

Gly Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Asn Gly Cys

20 25 30

Thr Val Asp Trp Ser Lys Ala Ile Gly Ile Ile Gly Asn Asn Ala Ala

35 40 45

Ala Asn Leu Thr Thr Gly Gly Ala Ala Gly Trp Asn Lys Gly

50 55 60

<210> 239

<211> 189

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 239

atgaaaactg ttaaagaact tagcgttaaa gaaatgcaac taactacagg aggtaagtat 60

tacggaaatg gcgtttcctg taataaaaat ggttgtactg tagattggag caaagctatt 120

gggattatag gaaacaatgc agcagcaaat ttgactacag gtggagccgc tggttggaac 180

aaaggataa 189

<210> 240

<211> 69

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<220>

<221> VARIANT

<222> 40

<223> Xaa =任意氨基酸

<400> 240

Met Tyr Lys Glu Leu Thr Val Asp Glu Leu Ala Leu Ile Asp Gly Gly

1 5 10 15

Lys Lys Lys Lys Lys Lys Val Ala Cys Thr Trp Gly Asn Ala Ala Thr

20 25 30

Ala Ala Ala Ser Gly Ala Val Xaa Gly Ile Leu Gly Gly Pro Thr Gly

35 40 45

Ala Leu Ala Gly Ala Ile Trp Gly Val Ser Gln Cys Ala Ser Asn Asn

50 55 60

Leu His Gly Met His

65

<210> 241

<211> 210

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<220>

<221> misc_feature

<222> 119

<223> n = a、c、t或g

<400> 241

atgtataaag aattaacagt tgatgaatta gcattgattg atggaggaaa aaagaagaag 60

aaaaaagtag cttgtacttg gggaaatgca gcaacagccg ctgcttctgg tgcagttang 120

ggtattcttg gtgggcctac tggtgcactg gctggagcta tctggggcgt ttcacaatgc 180

gcgtctaaca acttacacgg catgcactaa 210

<210> 242

<211> 56

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 242

Met Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile

1 5 10 15

Ile Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser

20 25 30

Cys Ser Val Asn Trp Gly Gln Ala Phe Ser Cys Ser Val Ser His Leu

35 40 45

Ala Asn Phe Gly His Gly Lys Cys

50 55

<210> 243

<211> 171

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 243

atgatgaaaa aaattgaaaa attaactgaa aaagaaatgg ccaatatcat tggtggtaaa 60

tactatggta atggggttac ttgtggtaaa cattcctgct ctgttaactg gggccaagca 120

ttttcttgta gtgtgtcaca tttagctaac ttcggtcatg gaaagtgcta a 171

<210> 244

<211> 64

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 244

Met Ser Lys Leu Val Lys Thr Leu Thr Val Asp Glu Ile Ser Lys Ile

1 5 10 15

Gln Thr Asn Gly Gly Lys Pro Ala Trp Cys Trp Tyr Thr Leu Ala Met

20 25 30

Cys Gly Ala Gly Tyr Asp Ser Gly Thr Cys Asp Tyr Met Tyr Ser His

35 40 45

Cys Phe Gly Val Lys His Ser Ser Gly Gly Gly Gly Ser Tyr His Cys

50 55 60

<210> 245

<211> 195

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 245

atgagtaaac tagttaaaac attaactgtc gatgaaatct ctaagattca aaccaatggt 60

ggaaaacctg catggtgttg gtacacattg gcaatgtgcg gtgctggtta tgattcaggc 120

acttgtgatt atatgtattc acactgcttt ggtgtaaaac actctagcgg tggtggcggt 180

agctaccatt gttag 195

<210> 246

<211> 56

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 246

Met Leu Gln Phe Glu Lys Leu Gln Tyr Ser Arg Leu Pro Gln Lys Lys

1 5 10 15

Leu Ala Lys Ile Ser Gly Gly Phe Asn Arg Gly Gly Tyr Asn Phe Gly

20 25 30

Lys Ser Val Arg His Val Val Asp Ala Ile Gly Ser Val Ala Gly Ile

35 40 45

Arg Gly Ile Leu Lys Ser Ile Arg

50 55

<210> 247

<211> 171

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 247

atgctacagt ttgagaaatt acaatattcc aggttgccgc aaaaaaagct tgccaaaata 60

tctggtggtt ttaatcgggg cggttataac tttggtaaaa gtgttcgaca tgttgttgat 120

gcaattggtt cagttgcagg cattcgtggt attttgaaaa gtattcgtta a 171

<210> 248

<211> 52

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 248

Met Lys Lys Phe Leu Val Leu Arg Asp Arg Glu Leu Asn Ala Ile Ser

1 5 10 15

Gly Gly Val Phe His Ala Tyr Ser Ala Arg Gly Val Arg Asn Asn Tyr

20 25 30

Lys Ser Ala Val Gly Pro Ala Asp Trp Val Ile Ser Ala Val Arg Gly

35 40 45

Phe Ile His Gly

50

<210> 249

<211> 159

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 249

atgaaaaaat ttctagtttt gcgtgaccgt gaattaaatg ctatttcagg tggcgttttc 60

catgcctata gcgcgcgtgg cgttcggaat aattataaaa gtgctgttgg gcctgccgat 120

tgggtcatta gcgctgtccg aggattcatc cacggatag 159

<210> 250

<211> 55

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 250

Met Thr Val Asn Lys Met Ile Lys Asp Leu Asp Val Val Asp Ala Phe

1 5 10 15

Ala Pro Ile Ser Asn Asn Lys Leu Asn Gly Val Val Gly Gly Gly Ala

20 25 30

Trp Lys Asn Phe Trp Ser Ser Leu Arg Lys Gly Phe Tyr Asp Gly Glu

35 40 45

Ala Gly Arg Ala Ile Arg Arg

50 55

<210> 251

<211> 168

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 251

atgactgtga acaaaatgat taaggatttg gatgtagtag atgcatttgc acctatttct 60

aataataagt tgaacggggt tgttggggga ggcgcttgga aaaatttctg gtctagttta 120

agaaaaggat tttatgatgg cgaagctggc agagcaatcc gtcgttaa 168

<210> 252

<211> 57

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 252

Met Lys Ile Lys Leu Thr Val Leu Asn Glu Phe Glu Glu Leu Thr Ala

1 5 10 15

Asp Ala Glu Lys Asn Ile Ser Gly Gly Arg Arg Ser Arg Lys Asn Gly

20 25 30

Ile Gly Tyr Ala Ile Gly Tyr Ala Phe Gly Ala Val Glu Arg Ala Val

35 40 45

Leu Gly Gly Ser Arg Asp Tyr Asn Lys

50 55

<210> 253

<211> 174

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 253

atgaaaatta aattaactgt tttaaatgaa tttgaagaat taactgctga cgctgaaaag 60

aatatttctg gtggccgtcg gagtcgtaaa aatggaattg gatacgctat tggttatgcg 120

tttggcgcgg ttgaacgggc cgtgcttggt ggttcaaggg attataataa gtga 174

<210> 254

<211> 47

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 254

Met Asp Lys Phe Glu Lys Ile Ser Thr Ser Asn Leu Glu Lys Ile Ser

1 5 10 15

Gly Gly Asp Leu Thr Thr Lys Leu Trp Ser Ser Trp Gly Tyr Tyr Leu

20 25 30

Gly Lys Lys Ala Arg Trp Asn Leu Lys His Pro Tyr Val Gln Phe

35 40 45

<210> 255

<211> 141

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 255

atggataaat ttgaaaaaat tagtacatct aacctagaaa agatctctgg cggtgattta 60

acaaccaagt tatggagctc ttggggatat tatcttggca agaaagcacg ttggaattta 120

aagcacccat atgttcaatt t 141

<210> 256

<211> 55

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 256

Met Asn Asn Leu Asn Lys Phe Ser Thr Leu Gly Lys Ser Ser Leu Ser

1 5 10 15

Gln Ile Glu Gly Gly Ser Val Pro Thr Ser Val Tyr Thr Leu Gly Ile

20 25 30

Lys Ile Leu Trp Ser Ala Tyr Lys His Arg Lys Thr Ile Glu Lys Ser

35 40 45

Phe Asn Lys Gly Phe Tyr His

50 55

<210> 257

<211> 168

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 257

atgaataact tgaataaatt ttctactcta ggcaagagta gcttgtctca aattgagggc 60

ggatcagtcc caacttcagt atatacgctt ggaattaaaa ttctatggtc tgcgtataag 120

catcgcaaaa cgattgaaaa aagttttaat aaaggctttt atcattaa 168

<210> 258

<211> 55

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 258

Met Asn Asn Ala Leu Ser Phe Glu Gln Gln Phe Thr Asp Phe Ser Thr

1 5 10 15

Leu Ser Asp Ser Glu Leu Glu Ser Val Glu Gly Gly Arg Asn Lys Leu

20 25 30

Ala Tyr Asn Met Gly His Tyr Ala Gly Lys Ala Thr Ile Phe Gly Leu

35 40 45

Ala Ala Trp Ala Leu Leu Ala

50 55

<210> 259

<211> 168

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 259

atgaataacg cattaagttt tgaacaacaa tttacagact tcagcacctt atcggactct 60

gaattagaat ccgttgaggg tggccgaaat aagcttgcat ataatatggg gcattacgct 120

ggtaaggcaa ccatttttgg acttgcagca tgggcactcc ttgcatga 168

<210> 260

<211> 47

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 260

Met Asp Lys Ile Ile Lys Phe Gln Gly Ile Ser Asp Asp Gln Leu Asn

1 5 10 15

Ala Val Ile Gly Gly Lys Lys Lys Lys Gln Ser Trp Tyr Ala Ala Ala

20 25 30

Gly Asp Ala Ile Val Ser Phe Gly Glu Gly Phe Leu Asn Ala Trp

35 40 45

<210> 261

<211> 144

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 261

atggataaga ttattaagtt tcaagggatt tctgatgatc aattaaatgc tgttatcggt 60

gggaaaaaga aaaaacaatc ttggtacgca gcagctggtg atgcaatcgt tagttttggt 120

gaaggatttt taaatgcttg gtaa 144

<210> 262

<211> 59

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 262

Met Lys Ile Ser Lys Ile Glu Ala Gln Ala Arg Lys Asp Phe Phe Lys

1 5 10 15

Lys Ile Asp Thr Asn Ser Asn Leu Leu Asn Val Asn Gly Ala Lys Cys

20 25 30

Lys Trp Trp Asn Ile Ser Cys Asp Leu Gly Asn Asn Gly His Val Cys

35 40 45

Thr Leu Ser His Glu Cys Gln Val Ser Cys Asn

50 55

<210> 263

<211> 180

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 263

atgaaaattt ctaagattga agctcaggct cgtaaagatt tttttaaaaa aatcgatact 60

aactcgaact tattaaatgt aaatggtgcc aaatgcaagt ggtggaatat ttcgtgtgat 120

ttaggaaata atggccatgt ttgtaccttg tcacatgaat gccaagtatc ttgtaactaa 180

<210> 264

<211> 67

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 264

Met Thr Lys Thr Ser Arg Arg Lys Asn Ala Ile Ala Asn Tyr Leu Glu

1 5 10 15

Pro Val Asp Glu Lys Ser Ile Asn Glu Ser Phe Gly Ala Gly Asp Pro

20 25 30

Glu Ala Arg Ser Gly Ile Pro Cys Thr Ile Gly Ala Ala Val Ala Ala

35 40 45

Ser Ile Ala Val Cys Pro Thr Thr Lys Cys Ser Lys Arg Cys Gly Lys

50 55 60

Arg Lys Lys

65

<210> 265

<211> 204

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 265

atgactaaaa ctagtcgtcg taagaatgct attgctaatt atttagaacc agtcgacgaa 60

aaaagtatta atgaatcttt tggggctggg gatccggaag caagatccgg aattccatgt 120

acaatcggcg cagctgtcgc agcatcaatt gcagtttgtc caactactaa gtgtagtaaa 180

cgttgtggca agcgtaagaa ataa 204

<210> 266

<211> 48

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 266

Met Lys Ile Gln Ile Lys Gly Met Lys Gln Leu Ser Asn Lys Glu Met

1 5 10 15

Gln Lys Ile Val Gly Gly Lys Ser Ser Ala Tyr Ser Leu Gln Met Gly

20 25 30

Ala Thr Ala Ile Lys Gln Val Lys Lys Leu Phe Lys Lys Trp Gly Trp

35 40 45

<210> 267

<211> 147

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 267

atgaaaattc aaattaaagg tatgaagcaa cttagtaata aggaaatgca aaaaatagta 60

ggtggaaaga gtagtgcgta ttctttgcag atgggggcaa ctgcaattaa acaggtaaag 120

aaactgttta aaaaatgggg atggtaa 147

<210> 268

<211> 207

<212> PRT

<213> 詹氏丙酸杆菌(Propionibacterium jensenii)

<400> 268

Met Asn Lys Thr His Lys Met Ala Thr Leu Val Ile Ala Ala Ile Leu

1 5 10 15

Ala Ala Gly Met Thr Ala Pro Thr Ala Tyr Ala Asp Ser Pro Gly Asn

20 25 30

Thr Arg Ile Thr Ala Ser Glu Gln Ser Val Leu Thr Gln Ile Leu Gly

35 40 45

His Lys Pro Thr Gln Thr Glu Tyr Asn Arg Tyr Val Glu Thr Tyr Gly

50 55 60

Ser Val Pro Thr Glu Ala Asp Ile Asn Ala Tyr Ile Glu Ala Ser Glu

65 70 75 80

Ser Glu Gly Ser Ser Ser Gln Thr Ala Ala His Asp Asp Ser Thr Ser

85 90 95

Pro Gly Thr Ser Thr Glu Ile Tyr Thr Gln Ala Ala Pro Ala Arg Phe

100 105 110

Ser Met Phe Phe Leu Ser Gly Thr Trp Ile Thr Arg Ser Gly Val Val

115 120 125

Ser Leu Ser Leu Lys Pro Arg Lys Gly Gly Ile Gly Asn Glu Gly Asp

130 135 140

Glu Arg Thr Trp Lys Thr Val Tyr Asp Lys Phe His Asn Ala Gly Gln

145 150 155 160

Trp Thr Arg Tyr Lys Asn Asn Gly Val Asp Ala Ser Met Lys Lys Gln

165 170 175

Tyr Met Cys His Phe Lys Tyr Gly Met Val Lys Thr Pro Trp Asn Leu

180 185 190

Glu Pro His Lys Lys Ala Ala Asp Val Ser Pro Val Lys Cys Asn

195 200 205

<210> 269

<211> 624

<212> DNA

<213> 詹氏丙酸杆菌(Propionibacterium jensenii)

<400> 269

atgaacaaaa cacacaaaat ggcgacgctg gtaattgccg cgatcttggc cgccggaatg 60

accgcaccaa ctgcctatgc agattctcct ggaaacacca gaattacagc cagcgagcaa 120

agcgtcctta cccagatact cggccacaaa cctacacaaa ctgaatataa ccgatacgtt 180

gagacttacg gaagcgtacc gaccgaagca gacatcaacg catatataga agcgtctgaa 240

tctgagggat catcaagtca aacggctgct cacgatgact cgacatcacc cggcacgagt 300

accgaaatct acacgcaggc agcccctgcc aggttctcaa tgtttttcct gtccggaact 360

tggatcacta ggagtggtgt agtatcgctc tccttgaagc caaggaaggg tggtattggc 420

aacgaggggg acgagcgtac ctggaagact gtatacgaca aattccataa cgctgggcaa 480

tggacacgat acaagaacaa cggcgtagac gccagcatga aaaagcagta catgtgccac 540

ttcaagtacg ggatggtgaa gacgccatgg aatctggagc cccacaagaa ggctgcagac 600

gtcagtccag tcaagtgcaa ctag 624

<210> 270

<211> 96

<212> PRT

<213> 特氏丙酸杆菌(Propionibacterium thoenii)

<400> 270

Met Lys Lys Thr Leu Leu Arg Ser Gly Thr Ile Ala Leu Ala Thr Ala

1 5 10 15

Ala Ala Phe Gly Ala Ser Leu Ala Ala Ala Pro Ser Ala Met Ala Val

20 25 30

Pro Gly Gly Cys Thr Tyr Thr Arg Ser Asn Arg Asp Val Ile Gly Thr

35 40 45

Cys Lys Thr Gly Ser Gly Gln Phe Arg Ile Arg Leu Asp Cys Asn Asn

50 55 60

Ala Pro Asp Lys Thr Ser Val Trp Ala Lys Pro Lys Val Met Val Ser

65 70 75 80

Val His Cys Leu Val Gly Gln Pro Arg Ser Ile Ser Phe Glu Thr Lys

85 90 95

<210> 271

<211> 291

<212> DNA

<213> 特氏丙酸杆菌(Propionibacterium thoenii)

<400> 271

atgaagaaga ccctcctgcg aagtggaacg atcgcactgg cgaccgcggc tgcatttggc 60

gcatcattgg cagccgcccc atctgccatg gccgttcctg gtggttgcac gtacacaaga 120

agcaatcgcg acgtcatcgg tacctgcaag actggaagcg gccagttccg aatccgactt 180

gactgcaaca acgctccaga caaaacttca gtctgggcca agcccaaggt aatggtgtcg 240

gttcactgtc ttgttggtca accgaggtcc atctcgttcg agaccaagtg a 291

<210> 272

<211> 255

<212> PRT

<213> 费氏丙酸杆菌谢氏亚种(Propionibacterium freudenreichii subspfreudenreii)

<400> 272

Met Asn Thr Lys Ala Val Asn Leu Lys Ser Glu Asn Thr Thr Lys Leu

1 5 10 15

Val Ser Tyr Leu Thr Glu Asn Gln Leu Asp Glu Phe Ile Arg Arg Ile

20 25 30

Arg Ile Asp Gly Ala Leu Val Glu Glu Val Ser Gln Asn Ala Lys Gln

35 40 45

Ala Leu Asp Asn Thr Gly Leu Asn Gly Trp Ile Asn Thr Asp Cys Asp

50 55 60

Glu Gly Leu Leu Ser Asp Phe Ile Ser Lys Ile Ala Ser Ala Arg Trp

65 70 75 80

Ile Pro Leu Ala Glu Ser Ile Arg Pro Ala Val Thr Asp Arg Asp Lys

85 90 95

Tyr Arg Val Ser Cys Trp Phe Tyr Gln Gly Met Asn Ile Ala Ile Tyr

100 105 110

Ala Asn Ile Gly Gly Val Ala Asn Ile Ile Gly Tyr Thr Glu Ala Ala

115 120 125

Val Ala Thr Leu Leu Gly Ala Val Val Ala Val Ala Pro Val Val Pro

130 135 140

Gly Thr Pro Thr Pro Pro Lys Asp Lys Ser Ser Gln Tyr Lys Glu Val

145 150 155 160

Pro Leu Ala Val Arg Leu Ser Glu Thr Tyr His Glu Glu Gly Val Arg

165 170 175

Gly Leu Phe Asp Glu Leu Asn Tyr Ser Glu Ser Arg Met Ile Ser Thr

180 185 190

Leu Arg Arg Ala Ser Thr Asp Gly Val Leu Ile Asn Ser Trp Asn Asp

195 200 205

Gly Gln Asp Thr Ile Leu Leu Lys Lys Tyr Asn Phe Gln Asp Leu Gln

210 215 220

Leu Thr Val Arg Ser Arg Ile Val Gly Asn Gln Thr Ile Ile Glu Glu

225 230 235 240

Cys Lys Ile Thr Asp Gly Arg Lys Thr Leu Ser Asp Glu Thr Val

245 250 255

<210> 273

<211> 768

<212> DNA

<213> 费氏丙酸杆菌谢氏亚种(Propionibacterium freudenreichii subspfreudenreii)

<400> 273

atgaatacca aagctgtaaa tctgaagtca gaaaacacga ctaagttggt gagctacctt 60

acggaaaatc aattggatga gtttattaga aggattcgca ttgatggcgc tcttgtggaa 120

gaggtcagtc aaaatgctaa gcaggcctta gataatactg ggctcaatgg ctggataaat 180

actgattgcg atgaaggcct tctctctgat ttcatttcaa agatagcaag tgctagatgg 240

attccattag ctgagtcaat tcgacctgcg gtgactgaca gggataagta tcgagtaagt 300

tgctggttct accaggggat gaatatagca atttacgcaa atatcggtgg cgtggccaat 360

attatcggct atacggaggc cgcagtcgca acactccttg gtgcagttgt ggcggtagct 420

cctgtggtcc ctggaactcc aacccctcca aaggacaaga gttcgcaata taaggaggtt 480

ccccttgccg ttcgtctttc cgaaacatac cacgaagagg gagtacgagg tctattcgac 540

gagctgaact actccgagag ccgtatgatc tctactctaa ggcgagcatc aaccgatgga 600

gtcctaatta attcttggaa cgatgggcag gatacaattc tgcttaagaa gtacaatttc 660

caagacttgc aactgactgt caggagccgc attgttggga atcaaacaat aattgaagaa 720

tgcaaaatca ctgatggtag aaaaactctt tcagacgaga ctgtgtag 768

<210> 274

<211> 618

<212> PRT

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 274

Met Ala Arg Pro Ile Ala Asp Leu Ile His Phe Asn Ser Thr Thr Val

1 5 10 15

Thr Ala Ser Gly Asp Val Tyr Tyr Gly Pro Gly Gly Gly Thr Gly Ile

20 25 30

Gly Pro Ile Ala Arg Pro Ile Glu His Gly Leu Asp Ser Ser Thr Glu

35 40 45

Asn Gly Trp Gln Glu Phe Glu Ser Tyr Ala Asp Val Gly Val Asp Pro

50 55 60

Arg Arg Tyr Val Pro Leu Gln Val Lys Glu Lys Arg Arg Glu Ile Glu

65 70 75 80

Leu Gln Phe Arg Asp Ala Glu Lys Lys Leu Glu Ala Ser Val Gln Ala

85 90 95

Glu Leu Asp Lys Ala Asp Ala Ala Leu Gly Pro Ala Lys Asn Leu Ala

100 105 110

Pro Leu Asp Val Ile Asn Arg Ser Leu Thr Ile Val Gly Asn Ala Leu

115 120 125

Gln Gln Lys Asn Gln Lys Leu Leu Leu Asn Gln Lys Lys Ile Thr Ser

130 135 140

Leu Gly Ala Lys Asn Phe Leu Thr Arg Thr Ala Glu Glu Ile Gly Glu

145 150 155 160

Gln Ala Val Arg Glu Gly Asn Ile Asn Gly Pro Glu Ala Tyr Met Arg

165 170 175

Phe Leu Asp Arg Glu Met Glu Gly Leu Thr Ala Ala Tyr Asn Val Lys

180 185 190

Leu Phe Thr Glu Ala Ile Ser Ser Leu Gln Ile Arg Met Asn Thr Leu

195 200 205

Thr Ala Ala Lys Ala Ser Ile Glu Ala Ala Ala Ala Asn Lys Ala Arg

210 215 220

Glu Gln Ala Ala Ala Glu Ala Lys Arg Lys Ala Glu Glu Gln Ala Arg

225 230 235 240

Gln Gln Ala Ala Ile Arg Ala Ala Asn Thr Tyr Ala Met Pro Ala Asn

245 250 255

Gly Ser Val Val Ala Thr Ala Ala Gly Arg Gly Leu Ile Gln Val Ala

260 265 270

Gln Gly Ala Ala Ser Leu Ala Gln Ala Ile Ser Asp Ala Ile Ala Val

275 280 285

Leu Gly Arg Val Leu Ala Ser Ala Pro Ser Val Met Ala Val Gly Phe

290 295 300

Ala Ser Leu Thr Tyr Ser Ser Arg Thr Ala Glu Gln Trp Gln Asp Gln

305 310 315 320

Thr Pro Asp Ser Val Arg Tyr Ala Leu Gly Met Asp Ala Ala Lys Leu

325 330 335

Gly Leu Pro Pro Ser Val Asn Leu Asn Ala Val Ala Lys Ala Ser Gly

340 345 350

Thr Val Asp Leu Pro Met Arg Leu Thr Asn Glu Ala Arg Gly Asn Thr

355 360 365

Thr Thr Leu Ser Val Val Ser Thr Asp Gly Val Ser Val Pro Lys Ala

370 375 380

Val Pro Val Arg Met Ala Ala Tyr Asn Ala Thr Thr Gly Leu Tyr Glu

385 390 395 400

Val Thr Val Pro Ser Thr Thr Ala Glu Ala Pro Pro Leu Ile Leu Thr

405 410 415

Trp Thr Pro Ala Ser Pro Pro Gly Asn Gln Asn Pro Ser Ser Thr Thr

420 425 430

Pro Val Val Pro Lys Pro Val Pro Val Tyr Glu Gly Ala Thr Leu Thr

435 440 445

Pro Val Lys Ala Thr Pro Glu Thr Tyr Pro Gly Val Ile Thr Leu Pro

450 455 460

Glu Asp Leu Ile Ile Gly Phe Pro Ala Asp Ser Gly Ile Lys Pro Ile

465 470 475 480

Tyr Val Met Phe Arg Asp Pro Arg Asp Val Pro Gly Ala Ala Thr Gly

485 490 495

Lys Gly Gln Pro Val Ser Gly Asn Trp Leu Gly Ala Ala Ser Gln Gly

500 505 510

Glu Gly Ala Pro Ile Pro Ser Gln Ile Ala Asp Lys Leu Arg Gly Lys

515 520 525

Thr Phe Lys Asn Trp Arg Asp Phe Arg Glu Gln Phe Trp Ile Ala Val

530 535 540

Ala Asn Asp Pro Glu Leu Ser Lys Gln Phe Asn Pro Gly Ser Leu Ala

545 550 555 560

Val Met Arg Asp Gly Gly Ala Pro Tyr Val Arg Glu Ser Glu Gln Ala

565 570 575

Gly Gly Arg Ile Lys Ile Glu Ile His His Lys Val Arg Val Ala Asp

580 585 590

Gly Gly Gly Val Tyr Asn Met Gly Asn Leu Val Ala Val Thr Pro Lys

595 600 605

Arg His Ile Glu Ile His Lys Gly Gly Lys

610 615

<210> 275

<211> 1857

<212> DNA

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 275

atggcacgac ccattgctga ccttatccac ttcaactcta caactgtcac ggcaagcgga 60

gacgtttatt acggccctgg gggaggtacc ggcattggcc ccattgccag acctatagag 120

cacggcttgg attcgtccac tgaaaatggc tggcaagagt ttgaaagtta tgctgatgtg 180

ggcgttgacc ccagacgcta tgttcctctt caggttaaag aaaaacgcag ggagatcgag 240

cttcagttcc gagatgccga gaaaaaactt gaggcgtcgg tacaagccga gctggataag 300

gctgatgccg ctcttggtcc ggcaaagaat cttgcaccat tggacgtcat caaccgcagt 360

ctgaccatcg ttggaaacgc cctccagcaa aagaatcaaa aactactgct gaatcagaag 420

aagattacca gcctgggtgc aaagaatttc cttacccgta cggcggaaga gatcggtgaa 480

caagcggtgc gagaaggcaa tattaacggg cctgaagcct atatgcgctt cctcgacagg 540

gaaatggaag gtctcacggc agcttataac gtaaaactct tcaccgaagc gatcagtagt 600

ctccagatcc gcatgaatac gttgaccgcc gccaaagcaa gtattgaggc ggccgcagca 660

aacaaggcgc gtgaacaagc agcggctgag gccaaacgca aagccgaaga gcaggcccgc 720

cagcaagcgg cgataagagc tgccaatacc tatgccatgc cggccaatgg cagcgttgtc 780

gccaccgccg caggccgggg tctgatccag gtcgcacaag gcgccgcatc ccttgctcaa 840

gcgatctccg atgcgattgc cgtcctgggc cgggtcctgg cttcagcacc ctcggtgatg 900

gccgtgggct ttgccagtct gacctactcc tcccggactg ccgagcaatg gcaggaccaa 960

acgcccgata gcgttcgtta cgccctgggc atggatgccg ctaaattggg gcttccccca 1020

agcgtaaacc tgaacgcggt tgcaaaagcc agcggtaccg tcgatctgcc gatgcgcctg 1080

accaacgagg cacgaggcaa cacgacgacc ctttcggtgg tcagcaccga tggtgtgagc 1140

gttccgaaag ccgttccggt ccggatggcg gcctacaatg ccacgacagg cctgtacgag 1200

gttacggttc cctctacgac cgcagaagcg ccgccactga tcctgacctg gacgccggcg 1260

agtcctccag gaaaccagaa cccttcgagt accactccgg tcgtaccgaa gccggtgccg 1320

gtatatgagg gagcgaccct tacaccggtg aaggctaccc cggaaaccta tcctggggtg 1380

attacactac cggaagacct gatcatcggc ttcccggccg actcggggat caagccgatc 1440

tatgtgatgt tcagggatcc gcgggatgta cctggtgctg cgactggcaa gggacagccc 1500

gtcagcggta attggctcgg cgccgcctct caaggtgagg gggctccaat tccaagccag 1560

attgcggata aactacgtgg taagacattc aaaaactggc gggactttcg ggaacaattc 1620

tggatagctg tggctaatga tcctgagtta agtaaacagt ttaatcctgg tagtttagct 1680

gtaatgagag atggaggggc tccttatgtc agagagtcag aacaggctgg cgggagaata 1740

aagatcgaaa tccaccacaa ggttcgagta gcagatggag gcggcgttta caatatgggg 1800

aaccttgttg cagtaacgcc aaaacgtcat atagaaatcc acaagggagg gaagtga 1857

<210> 276

<211> 689

<212> PRT

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 276

Met Ala Val Asn Asp Tyr Glu Pro Gly Ser Met Val Ile Thr His Val

1 5 10 15

Gln Gly Gly Gly Arg Asp Ile Ile Gln Tyr Ile Pro Ala Arg Ser Ser

20 25 30

Tyr Gly Thr Pro Pro Phe Val Pro Pro Gly Pro Ser Pro Tyr Val Gly

35 40 45

Thr Gly Met Gln Glu Tyr Arg Lys Leu Arg Ser Thr Leu Asp Lys Ser

50 55 60

His Ser Glu Leu Lys Lys Asn Leu Lys Asn Glu Thr Leu Lys Glu Val

65 70 75 80

Asp Glu Leu Lys Ser Glu Ala Gly Leu Pro Gly Lys Ala Val Ser Ala

85 90 95

Asn Asp Ile Arg Asp Glu Lys Ser Ile Val Asp Ala Leu Met Asp Ala

100 105 110

Lys Ala Lys Ser Leu Lys Ala Ile Glu Asp Arg Pro Ala Asn Leu Tyr

115 120 125

Thr Ala Ser Asp Phe Pro Gln Lys Ser Glu Ser Met Tyr Gln Ser Gln

130 135 140

Leu Leu Ala Ser Arg Lys Phe Tyr Gly Glu Phe Leu Asp Arg His Met

145 150 155 160

Ser Glu Leu Ala Lys Ala Tyr Ser Ala Asp Ile Tyr Lys Ala Gln Ile

165 170 175

Ala Ile Leu Lys Gln Thr Ser Gln Glu Leu Glu Asn Lys Ala Arg Ser

180 185 190

Leu Glu Ala Glu Ala Gln Arg Ala Ala Ala Glu Val Glu Ala Asp Tyr

195 200 205

Lys Ala Arg Lys Ala Asn Val Glu Lys Lys Val Gln Ser Glu Leu Asp

210 215 220

Gln Ala Gly Asn Ala Leu Pro Gln Leu Thr Asn Pro Thr Pro Glu Gln

225 230 235 240

Trp Leu Glu Arg Ala Thr Gln Leu Val Thr Gln Ala Ile Ala Asn Lys

245 250 255

Lys Lys Leu Gln Thr Ala Asn Asn Ala Leu Ile Ala Lys Ala Pro Asn

260 265 270

Ala Leu Glu Lys Gln Lys Ala Thr Tyr Asn Ala Asp Leu Leu Val Asp

275 280 285

Glu Ile Ala Ser Leu Gln Ala Arg Leu Asp Lys Leu Asn Ala Glu Thr

290 295 300

Ala Arg Arg Lys Glu Ile Ala Arg Gln Ala Ala Ile Arg Ala Ala Asn

305 310 315 320

Thr Tyr Ala Met Pro Ala Asn Gly Ser Val Val Ala Thr Ala Ala Gly

325 330 335

Arg Gly Leu Ile Gln Val Ala Gln Gly Ala Ala Ser Leu Ala Gln Ala

340 345 350

Ile Ser Asp Ala Ile Ala Val Leu Gly Arg Val Leu Ala Ser Ala Pro

355 360 365

Ser Val Met Ala Val Gly Phe Ala Ser Leu Thr Tyr Ser Ser Arg Thr

370 375 380

Ala Glu Gln Trp Gln Asp Gln Thr Pro Asp Ser Val Arg Tyr Ala Leu

385 390 395 400

Gly Met Asp Ala Ala Lys Leu Gly Leu Pro Pro Ser Val Asn Leu Asn

405 410 415

Ala Val Ala Lys Ala Ser Gly Thr Val Asp Leu Pro Met Arg Leu Thr

420 425 430

Asn Glu Ala Arg Gly Asn Thr Thr Thr Leu Ser Val Val Ser Thr Asp

435 440 445

Gly Val Ser Val Pro Lys Ala Val Pro Val Arg Met Ala Ala Tyr Asn

450 455 460

Ala Thr Thr Gly Leu Tyr Glu Val Thr Val Pro Ser Thr Thr Ala Glu

465 470 475 480

Ala Pro Pro Leu Ile Leu Thr Trp Thr Pro Ala Ser Pro Pro Gly Asn

485 490 495

Gln Asn Pro Ser Ser Thr Thr Pro Val Val Pro Lys Pro Val Pro Val

500 505 510

Tyr Glu Gly Ala Thr Leu Thr Pro Val Lys Ala Thr Pro Glu Thr Tyr

515 520 525

Pro Gly Val Ile Thr Leu Pro Glu Asp Leu Ile Ile Gly Phe Pro Ala

530 535 540

Asp Ser Gly Ile Lys Pro Ile Tyr Val Met Phe Arg Asp Pro Arg Asp

545 550 555 560

Val Pro Gly Ala Ala Thr Gly Lys Gly Gln Pro Val Ser Gly Asn Trp

565 570 575

Leu Gly Ala Ala Ser Gln Gly Glu Gly Ala Pro Ile Pro Ser Gln Ile

580 585 590

Ala Asp Lys Leu Arg Gly Lys Thr Phe Lys Asn Trp Arg Asp Phe Arg

595 600 605

Glu Gln Phe Trp Ile Ala Val Ala Asn Asp Pro Glu Leu Ser Lys Gln

610 615 620

Phe Asn Pro Gly Ser Leu Ala Val Met Arg Asp Gly Gly Ala Pro Tyr

625 630 635 640

Val Arg Glu Ser Glu Gln Ala Gly Gly Arg Ile Lys Ile Glu Ile His

645 650 655

His Lys Val Arg Ile Ala Asp Gly Gly Gly Val Tyr Asn Met Gly Asn

660 665 670

Leu Val Ala Val Thr Pro Lys Arg His Ile Glu Ile His Lys Gly Gly

675 680 685

Lys

<210> 277

<211> 2070

<212> DNA

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 277

atggctgtca atgattacga acctggttcg atggttatta cacatgtgca gggtggtggg 60

cgtgacataa tccagtatat tcctgctcga tcaagctacg gtactccacc atttgtccca 120

ccaggaccaa gtccgtatgt cggtactgga atgcaggagt acaggaagct aagaagtacg 180

cttgataagt cccattcaga actcaagaaa aacctgaaaa atgaaaccct gaaggaggtt 240

gatgaactca agagtgaagc ggggttgcca ggtaaagcgg tcagtgccaa tgacatccgc 300

gatgaaaaga gtatcgttga tgcactcatg gatgccaaag caaaatcgct aaaggccatt 360

gaggatcgcc cggccaatct ttatacggct tcagactttc ctcagaagtc agagtcgatg 420

taccagagtc agttgctggc cagccgaaaa ttctatggag agttcctgga tcgccatatg 480

agtgagctgg ccaaagcgta cagcgccgat atctataagg cgcaaatcgc tatcttgaaa 540

caaacgtctc aagagctgga gaataaagcc cggtcattgg aagcagaagc ccagcgagcc 600

gctgctgagg tggaggcgga ctacaaggcc aggaaggcaa atgtcgagaa aaaagtgcag 660

tccgagcttg accaggctgg gaatgctttg cctcaactga ccaatccaac gccagagcag 720

tggcttgaac gcgctactca actggttacg caggcgatcg ccaataagaa gaaattgcag 780

actgcaaaca atgccttgat tgccaaggca cccaatgcac tggagaaaca aaaggcaacc 840

tacaacgccg atctcctagt ggatgaaatc gccagcctgc aagcacggct ggacaagctg 900

aacgccgaaa cggcaaggcg caaggaaatc gctcgtcaag cggcgatcag ggctgccaat 960

acttatgcca tgccagccaa tggcagcgtt gtcgccaccg ccgcaggccg gggtctgatc 1020

caggtcgcac aaggcgccgc atcccttgct caagcgatct ccgatgcgat tgccgtcctg 1080

ggccgggtcc tggcttcagc accctcggtg atggccgtgg gctttgccag tctgacctac 1140

tcctcccgga ctgccgagca atggcaggac caaacgcccg atagcgttcg ttacgccctg 1200

ggcatggatg ccgctaaatt ggggcttccc ccaagcgtaa acctgaacgc ggttgcaaaa 1260

gccagcggta ccgtcgatct gccgatgcgc ctgaccaacg aggcacgagg caacacgacg 1320

accctttcgg tggtcagcac cgatggtgtg agcgttccga aagccgttcc ggtccggatg 1380

gcggcctaca atgccacgac aggcctgtac gaggttacgg ttccctctac gaccgcagaa 1440

gcgccgccac tgatcctgac ctggacgccg gcgagtcctc caggaaacca gaacccttcg 1500

agtaccactc cggtcgtacc gaagccggtg ccggtatatg agggagcgac ccttacaccg 1560

gtgaaggcta ccccggaaac ctatcctggg gtgattacac taccggaaga cctgatcatc 1620

ggcttcccgg ccgactcggg gatcaagccg atctatgtga tgttcaggga tccgcgggat 1680

gtacctggtg ctgcgactgg caagggacag cccgtcagcg gtaattggct cggcgccgcc 1740

tctcaaggtg agggggctcc aattccaagc cagattgcgg ataaactacg tggtaagaca 1800

ttcaaaaact ggcgggactt tcgggaacaa ttctggatag ctgtggctaa tgatcctgag 1860

ttaagtaaac agtttaatcc tggtagttta gctgtaatga gagatggagg ggctccttat 1920

gtcagagagt cagaacaggc tggcgggaga ataaagatcg aaatccacca caaggttcga 1980

atagcagatg gaggcggcgt ttacaatatg gggaaccttg ttgcagtaac gccaaaacgt 2040

catatagaaa tccacaaggg agggaagtga 2070

<210> 278

<211> 47

<212> PRT

<213> 活泼瘤胃球菌(Ruminococcus gnavus)

<400> 278

Met Arg Asn Asp Val Leu Thr Leu Thr Asn Pro Met Glu Glu Lys Glu

1 5 10 15

Leu Glu Gln Ile Leu Gly Gly Gly Asn Gly Val Leu Lys Thr Ile Ser

20 25 30

His Glu Cys Asn Met Asn Thr Trp Gln Phe Leu Phe Thr Cys Cys

35 40 45

<210> 279

<211> 144

<212> DNA

<213> 活泼瘤胃球菌(Ruminococcus gnavus)

<400> 279

atgagaaatg acgtattaac attaacaaac ccaatggaag agaacgaact ggagcagatc 60

ttaggtggtg gcaatggtgt gttaaaaacg attagccacg aatgcaatat gaacacatgg 120

cagttcctgt ttacttgttg ctaa 144

<210> 280

<211> 55

<212> PRT

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 280

Met Lys Asn Ala Lys Ser Leu Thr Ile Gln Glu Met Lys Ser Ile Thr

1 5 10 15

Gly Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Ser His Gly Cys

20 25 30

Ser Val Asn Trp Gly Gln Ala Trp Thr Cys Gly Val Asn His Leu Ala

35 40 45

Asn Gly Gly His Gly Val Cys

50 55

<210> 281

<211> 168

<212> DNA

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 281

atgaaaaacg caaaaagcct aacaattcaa gaaatgaaat ctattacagg tggtaaatac 60

tatggtaatg gcgttagctg taactctcac ggctgttcag taaattgggg gcaagcatgg 120

acttgtggag taaaccatct agctaatggc ggtcatggag tttgttaa 168

<210> 282

<211> 59

<212> PRT

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 282

Met Asn Asn Val Lys Glu Leu Ser Met Thr Glu Leu Gln Thr Ile Thr

1 5 10 15

Gly Gly Ala Arg Ser Tyr Gly Asn Gly Val Tyr Cys Asn Asn Lys Lys

20 25 30

Cys Trp Val Asn Arg Gly Glu Ala Thr Gln Ser Ile Ile Gly Gly Met

35 40 45

Ile Ser Gly Trp Ala Ser Gly Leu Ala Gly Met

50 55

<210> 283

<211> 180

<212> DNA

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 283

atgaataatg taaaagaatt aagtatgaca gaattacaaa caattaccgg cggtgctaga 60

tcatatggca acggtgttta ctgtaataat aaaaaatgtt gggtaaatcg gggtgaagca 120

acgcaaagta ttattggtgg tatgattagc ggctgggcta gtggtttagc tggaatgtaa 180

<210> 284

<211> 61

<212> PRT

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 284

Met Glu Lys Phe Ile Glu Leu Ser Leu Lys Glu Val Thr Ala Ile Thr

1 5 10 15

Gly Gly Lys Tyr Tyr Gly Asn Gly Val His Cys Gly Lys His Ser Cys

20 25 30

Thr Val Asp Trp Gly Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala

35 40 45

Ala Asn Trp Ala Thr Gly Gly Asn Ala Gly Trp Asn Lys

50 55 60

<210> 285

<211> 186

<212> DNA

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 285

atggaaaagt ttattgaatt atctttaaaa gaagtaacag caattacagg tggaaaatat 60

tatggtaacg gtgtacactg tggaaaacat tcatgtaccg tagactgggg aacagctatt 120

ggaaatatcg gaaataatgc agctgcaaac tgggccacag gcggaaacgc tggctggaat 180

aaataa 186

<210> 286

<211> 56

<212> PRT

<213> 唾液链球菌(Streptococcus salivarius)

<400> 286

Met Lys Ser Thr Asn Asn Gln Ser Ile Ala Glu Ile Ala Ala Val Asn

1 5 10 15

Ser Leu Gln Glu Val Ser Met Glu Glu Leu Asp Gln Ile Ile Gly Ala

20 25 30

Gly Asn Gly Val Val Leu Thr Leu Thr His Glu Cys Asn Leu Ala Thr

35 40 45

Trp Thr Lys Lys Leu Lys Cys Cys

50 55

<210> 287

<211> 171

<212> DNA

<213> 唾液链球菌(Streptococcus salivarius)

<400> 287

atgaaatcaa caaataatca aagtatcgca gaaattgcag cagtaaactc actacaagaa 60

gtaagtatgg aggaactaga ccaaattatt ggtgccggaa acggagtggt tcttactctt 120

actcatgaat gtaacctagc aacttggaca aaaaaactaa aatgttgcta a 171

<210> 288

<211> 51

<212> PRT

<213> 产脓链球菌血清型M28(Streptococcus pyogenes serotype M28)

<400> 288

Met Ser Phe Met Lys Asn Ser Lys Asp Ile Leu Thr Asn Ala Ile Glu

1 5 10 15

Glu Val Ser Glu Lys Glu Leu Met Glu Val Ala Gly Gly Lys Lys Gly

20 25 30

Ser Gly Trp Phe Ala Thr Ile Thr Asp Asp Cys Pro Asn Ser Val Phe

35 40 45

Val Cys Cys

50

<210> 289

<211> 156

<212> DNA

<213> 产脓链球菌血清型M28(Streptococcus pyogenes serotype M28)

<400> 289

atgagtttta tgaaaaattc aaaggatatt ttgactaatg ctatcgaaga agtttctgaa 60

aaagaactta tggaagtagc tggtggtaaa aaaggttccg gttggtttgc aactattact 120

gatgactgtc cgaactcagt attcgtttgt tgttaa 156

<210> 290

<211> 48

<212> PRT

<213> 唾液链球菌(Streptococcus salivarius)

<400> 290

Met Lys Asn Ser Lys Asp Val Leu Asn Asn Ala Ile Glu Glu Val Ser

1 5 10 15

Glu Lys Glu Leu Met Glu Val Ala Gly Gly Lys Lys Gly Pro Gly Trp

20 25 30

Ile Ala Thr Ile Thr Asp Asp Cys Pro Asn Ser Ile Phe Val Cys Cys

35 40 45

<210> 291

<211> 147

<212> DNA

<213> 唾液链球菌(Streptococcus salivarius)

<400> 291

atgaaaaact caaaagatgt tttgaacaat gctatcgaag aggtttctga aaaagaactt 60

atggaagtag ctggtggtaa aaaaggtcca ggttggattg caactattac tgatgactgt 120

ccaaactcaa tattcgtttg ttgttaa 147

<210> 292

<211> 48

<212> PRT

<213> 唾液链球菌(Streptococcus salivarius)

<400> 292

Met Lys Asn Ser Lys Asp Ile Leu Asn Asn Ala Ile Glu Glu Val Ser

1 5 10 15

Glu Lys Glu Leu Met Glu Val Ala Gly Gly Lys Arg Gly Ser Gly Trp

20 25 30

Ile Ala Thr Ile Thr Asp Asp Cys Pro Asn Ser Val Phe Val Cys Cys

35 40 45

<210> 293

<211> 147

<212> DNA

<213> 唾液链球菌(Streptococcus salivarius)

<400> 293

atgaaaaact caaaagatat tttgaacaat gctatcgaag aagtttctga aaaagaactt 60

atggaagtag ctggtggtaa aagaggttca ggttggattg caactattac tgatgactgt 120

ccaaactcag tattcgtttg ttgttaa 147

<210> 294

<211> 62

<212> PRT

<213> 金黄色葡萄球菌(Staphylococcus aureus)

<400> 294

Met Lys Ser Ser Phe Leu Glu Lys Asp Ile Glu Glu Gln Val Thr Trp

1 5 10 15

Phe Glu Glu Val Ser Glu Gln Glu Phe Asp Asp Asp Ile Phe Gly Ala

20 25 30

Cys Ser Thr Asn Thr Phe Ser Leu Ser Asp Tyr Trp Gly Asn Lys Gly

35 40 45

Asn Trp Cys Thr Ala Thr His Glu Cys Met Ser Trp Cys Lys

50 55 60

<210> 295

<211> 189

<212> DNA

<213> 金黄色葡萄球菌(Staphylococcus aureus)

<400> 295

atgaaaagtt cttttttaga aaaagatata gaagaacaag tgacatggtt cgaggaagtt 60

tcagaacaag aatttgacga tgatattttt ggagcttgta gtacaaacac tttttctttg 120

agtgactatt ggggtaataa aggaaattgg tgtactgcta ctcacgaatg tatgtcttgg 180

tgtaaataa 189

<210> 296

<211> 67

<212> PRT

<213> 金黄色葡萄球菌(Staphylococcus aureus)

<400> 296

Met Lys Asn Glu Leu Gly Lys Phe Leu Glu Glu Asn Glu Leu Glu Leu

1 5 10 15

Gly Lys Phe Ser Glu Ser Asp Met Leu Glu Ile Thr Asp Asp Glu Val

20 25 30

Tyr Ala Ala Gly Thr Pro Leu Ala Leu Leu Gly Gly Ala Ala Thr Gly

35 40 45

Val Ile Gly Tyr Ile Ser Asn Gln Thr Cys Pro Thr Thr Ala Cys Thr

50 55 60

Arg Ala Cys

65

<210> 297

<211> 204

<212> DNA

<213> 金黄色葡萄球菌(Staphylococcus aureus)

<400> 297

atgaaaaatg aattaggtaa gtttttagaa gaaaacgaat tagagttagg taaattttca 60

gaatcagaca tgctagaaat tactgatgat gaagtatatg cagctggaac acctttagcc 120

ttattgggtg gagctgccac cggggtgata ggttatattt ctaaccaaac atgtccaaca 180

actgcttgta cacgcgcttg ctag 204

<210> 298

<211> 46

<212> PRT

<213> 产脓链球菌(Streptococcus pyogenes)

<400> 298

Met Asn Asn Thr Ile Lys Asp Phe Asp Leu Asp Leu Lys Thr Asn Lys

1 5 10 15

Lys Asp Thr Ala Thr Pro Tyr Val Gly Ser Arg Tyr Leu Cys Thr Pro

20 25 30

Gly Ser Cys Trp Lys Leu Val Cys Phe Thr Thr Thr Val Lys

35 40 45

<210> 299

<211> 141

<212> DNA

<213> 产脓链球菌(Streptococcus pyogenes)

<400> 299

atgaataaca caattaaaga ctttgatctc gatttgaaaa caaataaaaa agacactgct 60

acaccttatg ttggtagccg ttacctatgt acccctggtt cttgttggaa attagtttgc 120

tttacaacaa ctgttaaata a 141

<210> 300

<211> 51

<212> PRT

<213> 产脓链球菌(Streptococcus pyogenes)

<400> 300

Met Glu Lys Asn Asn Glu Val Ile Asn Ser Ile Gln Glu Val Ser Leu

1 5 10 15

Glu Glu Leu Asp Gln Ile Ile Gly Ala Gly Lys Asn Gly Val Phe Lys

20 25 30

Thr Ile Ser His Glu Cys His Leu Asn Thr Trp Ala Phe Leu Ala Thr

35 40 45

Cys Cys Ser

50

<210> 301

<211> 156

<212> DNA

<213> 产脓链球菌(Streptococcus pyogenes)

<400> 301

atggaaaaaa ataatgaagt aatcaactct attcaagaag ttagtcttga agaactcgat 60

caaattatcg gtgctggaaa aaatggtgtg tttaaaacaa tttctcatga gtgtcatttg 120

aatacatggg cattccttgc tacttgttgt tcataa 156

<210> 302

<211> 51

<212> PRT

<213> 产脓链球菌血清型M49(Streptococcus pyogenes serotype M49)

<400> 302

Met Thr Lys Glu His Glu Ile Ile Asn Ser Ile Gln Glu Val Ser Leu

1 5 10 15

Glu Glu Leu Asp Gln Ile Ile Gly Ala Gly Lys Asn Gly Val Phe Lys

20 25 30

Thr Ile Ser His Glu Cys His Leu Asn Thr Trp Ala Phe Leu Ala Thr

35 40 45

Cys Cys Ser

50

<210> 303

<211> 156

<212> DNA

<213> 产脓链球菌血清型M49(Streptococcus pyogenes serotype M49)

<400> 303

atggaaaaaa ataatgaagt aatcaactct attcaagaag ttagtcttga agaactcgat 60

caaattatcg gtgctggaaa aaatggtgtg tttaaaacaa tttctcatga gtgtcatttg 120

aatacatggg cattccttgc tacttgttgc tcataa 156

<210> 304

<211> 56

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 304

Met Glu Lys Leu Phe Lys Glu Val Lys Leu Glu Glu Leu Glu Asn Gln

1 5 10 15

Lys Gly Ser Gly Leu Gly Lys Ala Gln Cys Ala Ala Leu Trp Leu Gln

20 25 30

Cys Ala Ser Gly Gly Thr Ile Gly Cys Gly Gly Gly Ala Val Ala Cys

35 40 45

Gln Asn Tyr Arg Gln Phe Cys Arg

50 55

<210> 305

<211> 171

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 305

atggaaaagc tatttaaaga agttaaacta gaggaactcg aaaaccaaaa aggtagtgga 60

ttaggaaaag ctcagtgtgc tgcgttgtgg ctacaatgtg ctagtggcgg tacaattggt 120

tgtggtggcg gagctgttgc ttgtcaaaac tatcgtcaat tctgcagata a 171

<210> 306

<211> 56

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 306

Met Ser Lys Phe Asp Asp Phe Asp Leu Asp Val Val Lys Val Ser Lys

1 5 10 15

Gln Asp Ser Lys Ile Thr Pro Gln Trp Lys Ser Glu Ser Leu Cys Thr

20 25 30

Pro Gly Cys Val Thr Gly Ala Leu Gln Thr Cys Phe Leu Gln Thr Leu

35 40 45

Thr Cys Asn Cys Lys Ile Ser Lys

50 55

<210> 307

<211> 171

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 307

atgtcaaagt tcgatgattt cgatttggat gttgtgaaag tctctaaaca agactcaaaa 60

atcactccgc aatggaaaag tgaatcactt tgtacaccag gatgtgtaac tggtgcattg 120

caaacttgct tccttcaaac actaacttgt aactgcaaaa tctctaaata a 171

<210> 308

<211> 50

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 308

Met Lys Leu Pro Val Gln Gln Val Tyr Ser Val Tyr Gly Gly Lys Asp

1 5 10 15

Leu Pro Lys Gly His Ser His Ser Thr Met Pro Phe Leu Ser Lys Leu

20 25 30

Gln Phe Leu Thr Lys Ile Tyr Leu Leu Asp Ile His Thr Gln Pro Phe

35 40 45

Phe Ile

50

<210> 309

<211> 153

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 309

ttgaaattgc cggtgcaaca ggtctattcg gtctatgggg gtaaggatct cccaaaaggg 60

catagtcatt ctactatgcc ctttttaagt aaattacaat ttttaactaa aatctacctc 120

ttggatatac atacacaacc gtttttcatt tga 153

<210> 310

<211> 43

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 310

Met Lys Lys Ala Val Ile Val Glu Asn Lys Gly Cys Ala Thr Cys Ser

1 5 10 15

Ile Gly Ala Ala Cys Leu Val Asp Gly Pro Ile Pro Asp Phe Glu Ile

20 25 30

Ala Gly Ala Thr Gly Leu Phe Gly Leu Trp Gly

35 40

<210> 311

<211> 132

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 311

atgaaaaaag ctgtcattgt agaaaacaaa ggttgtgcaa catgctcgat cggagccgct 60

tgtctagtgg acggtcctat ccctgatttt gaaattgccg gtgcaacagg tctattcggt 120

ctatgggggt aa 132

<210> 312

<211> 58

<212> PRT

<213> 嗜热乳酸链球菌(Streptococcus thermophilus)

<400> 312

Met Met Asn Ala Thr Glu Asn Gln Ile Phe Val Glu Thr Val Ser Asp

1 5 10 15

Gln Glu Leu Glu Met Leu Ile Gly Gly Ala Asp Arg Gly Trp Ile Lys

20 25 30

Thr Leu Thr Lys Asp Cys Pro Asn Val Ile Ser Ser Ile Cys Ala Gly

35 40 45

Thr Ile Ile Thr Ala Cys Lys Asn Cys Ala

50 55

<210> 313

<211> 177

<212> DNA

<213> 嗜热乳酸链球菌(Streptococcus thermophilus)

<400> 313

atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa 60

atgttaattg gtggtgcaga tcgtggatgg attaagactt taacaaaaga ttgtccaaat 120

gtaatttctt caatttgtgc aggtacaatt attacagcct gtaaaaattg tgcttaa 177

<210> 314

<211> 64

<212> PRT

<213> 嗜热乳酸链球菌(Streptococcus thermophilus)

<400> 314

Met Lys Gln Tyr Asn Gly Phe Glu Val Leu His Glu Leu Asp Leu Ala

1 5 10 15

Asn Val Thr Gly Gly Gln Ile Asn Trp Gly Ser Val Val Gly His Cys

20 25 30

Ile Gly Gly Ala Ile Ile Gly Gly Ala Phe Ser Gly Gly Ala Ala Ala

35 40 45

Gly Val Gly Cys Leu Val Gly Ser Gly Lys Ala Ile Ile Asn Gly Leu

50 55 60

<210> 315

<211> 195

<212> DNA

<213> 嗜热乳酸链球菌(Streptococcus thermophilus)

<400> 315

atgaagcagt ataatggttt tgaggttcta catgaacttg acttagcaaa tgtaactggc 60

ggtcaaatta attggggatc agttgtagga cactgtatag gtggagctat tatcggaggt 120

gcattttcag gaggtgcagc ggctggagta ggatgccttg ttgggagcgg aaaggcaatc 180

ataaatggat tataa 195

<210> 316

<211> 85

<212> PRT

<213> 嗜热乳酸链球菌(Streptococcus thermophilus)

<400> 316

Met Asn Thr Ile Thr Ile Cys Lys Phe Asp Val Leu Asp Ala Glu Leu

1 5 10 15

Leu Ser Thr Val Glu Gly Gly Tyr Ser Gly Lys Asp Cys Leu Lys Asp

20 25 30

Met Gly Gly Tyr Ala Leu Ala Gly Ala Gly Ser Gly Ala Leu Trp Gly

35 40 45

Ala Pro Ala Gly Gly Val Gly Ala Leu Pro Gly Ala Phe Val Gly Ala

50 55 60

His Val Gly Ala Ile Ala Gly Gly Phe Ala Cys Met Gly Gly Met Ile

65 70 75 80

Gly Asn Lys Phe Asn

85

<210> 317

<211> 258

<212> DNA

<213> 嗜热乳酸链球菌(Streptococcus thermophilus)

<400> 317

atgaatacaa taactatttg taaatttgat gttttagatg ctgaacttct ttcgacagtt 60

gagggtggat actctggtaa ggattgttta aaagacatgg gaggatatgc attggcagga 120

gctggaagtg gagctctgtg gggagctcca gcaggaggtg ttggagcact tccaggtgca 180

tttgtcggag ctcatgttgg ggcaattgca ggaggctttg catgtatggg tggaatgatt 240

ggtaataagt ttaactaa 258

<210> 318

<211> 52

<212> PRT

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 318

Met Ser Glu Ile Lys Lys Ala Leu Asn Thr Leu Glu Ile Glu Asp Phe

1 5 10 15

Asp Ala Ile Glu Met Val Asp Val Asp Ala Met Pro Glu Asn Glu Ala

20 25 30

Leu Glu Ile Met Gly Ala Ser Cys Thr Thr Cys Val Cys Thr Cys Ser

35 40 45

Cys Cys Thr Thr

50

<210> 319

<211> 159

<212> DNA

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 319

atgagtgaaa ttaaaaaagc attaaatacg cttgaaattg aagattttga tgcaattgaa 60

atggttgatg ttgatgctat gccagaaaac gaagcgcttg aaattatggg agcgtcatgt 120

acgacatgcg tatgtacatg cagttgttgt acaacttga 159

<210> 320

<211> 47

<212> PRT

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 320

Met Glu Val Met Asn Asn Ala Leu Ile Thr Lys Val Asp Glu Glu Ile

1 5 10 15

Gly Gly Asn Ala Ala Cys Val Ile Gly Cys Ile Gly Ser Cys Val Ile

20 25 30

Ser Glu Gly Ile Gly Ser Leu Val Gly Thr Ala Phe Thr Leu Gly

35 40 45

<210> 321

<211> 144

<212> DNA

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 321

atggaagtta tgaacaatgc tttaattaca aaagtagatg aggagattgg aggaaacgct 60

gcttgtgtaa ttggttgtat tggcagttgc gtaattagtg aaggaattgg ttcacttgta 120

ggaacagcat ttactttagg ttaa 144

<210> 322

<211> 49

<212> PRT

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 322

Met Glu Val Leu Asn Lys Gln Asn Val Asn Ile Ile Pro Glu Ser Glu

1 5 10 15

Glu Val Gly Gly Trp Val Ala Cys Val Gly Ala Cys Gly Thr Val Cys

20 25 30

Leu Ala Ser Gly Gly Val Gly Thr Glu Phe Ala Ala Ala Ser Tyr Phe

35 40 45

Leu

<210> 323

<211> 150

<212> DNA

<213> 蜡样芽孢杆菌(Bacillus cereus)

<400> 323

atggaagttt taaacaaaca aaatgtaaat attattccag aatctgaaga agtaggtgga 60

tgggtagcat gtgttggagc atgtggtaca gtatgtcttg ctagtggtgg tgttggaaca 120

gagtttgcag ctgcatctta tttcctataa 150

<210> 324

<211> 40

<212> PRT

<213> 苏云金芽孢杆菌(Bacillus thuringiensis)

<400> 324

Met Glu Thr Pro Val Val Gln Pro Arg Asp Trp Thr Cys Trp Ser Cys

1 5 10 15

Leu Val Cys Ala Ala Cys Ser Val Glu Leu Leu Asn Leu Val Thr Ala

20 25 30

Ala Thr Gly Ala Ser Thr Ala Ser

35 40

<210> 325

<211> 123

<212> DNA

<213> 苏云金芽孢杆菌(Bacillus thuringiensis)

<400> 325

atggaaacac cagtagtaca accaagggat tggacttgtt ggagttgctt agtatgtgca 60

gcatgttctg tggaattatt aaatttagtt actgcggcaa caggggctag tactgcaagc 120

taa 123

<210> 326

<211> 42

<212> PRT

<213> 根瘤菌三叶草生物型(Rhizobium leguminosarum bv. trifolii)

<400> 326

Met Asp Asn Lys Val Ala Lys Asn Val Glu Val Lys Lys Gly Ser Ile

1 5 10 15

Lys Ala Thr Phe Lys Ala Ala Val Leu Lys Ser Lys Thr Lys Val Asp

20 25 30

Ile Gly Gly Ser Arg Gln Gly Cys Val Ala

35 40

<210> 327

<211> 129

<212> DNA

<213> 根瘤菌三叶草生物型(Rhizobium leguminosarum bv. trifolii)

<400> 327

atggataaca aggttgcgaa gaatgtcgaa gtgaagaagg gctccatcaa ggcgaccttc 60

aaggctgctg ttctgaagtc gaagacgaag gtcgacatcg gaggtagccg tcagggctgc 120

gtcgcttaa 129

<210> 328

<211> 70

<212> PRT

<213> 乳房链球菌(Streptococcus uberis)

<400> 328

Met Asn Thr Ile Glu Lys Phe Glu Asn Ile Lys Leu Phe Ser Leu Lys

1 5 10 15

Lys Ile Ile Gly Gly Lys Thr Val Asn Tyr Gly Asn Gly Leu Tyr Cys

20 25 30

Asn Gln Lys Lys Cys Trp Val Asn Trp Ser Glu Thr Ala Thr Thr Ile

35 40 45

Val Asn Asn Ser Ile Met Asn Gly Leu Thr Gly Gly Asn Ala Gly Trp

50 55 60

His Ser Gly Gly Arg Ala

65 70

<210> 329

<211> 213

<212> DNA

<213> 乳房链球菌(Streptococcus uberis)

<400> 329

atgaatacaa ttgaaaaatt tgaaaatatt aaactttttt cactaaagaa aattatcggt 60

ggcaaaactg taaattatgg taatggcctt tattgtaacc aaaaaaaatg ctgggtaaac 120

tggtcagaaa ctgctacaac aatagtaaat aattccatca tgaacgggct cacaggtggt 180

aatgcgggtt ggcactcagg cgggagagca taa 213

<210> 330

<211> 76

<212> PRT

<213> 乳房链球菌(Streptococcus uberis)

<400> 330

Met Asp Ile Leu Leu Glu Leu Ala Gly Tyr Thr Gly Ile Ala Ser Gly

1 5 10 15

Thr Ala Lys Lys Val Val Asp Ala Ile Asp Lys Gly Ala Ala Ala Phe

20 25 30

Val Ile Ile Ser Ile Ile Ser Thr Val Ile Ser Ala Gly Ala Leu Gly

35 40 45

Ala Val Ser Ala Ser Ala Asp Phe Ile Ile Leu Thr Val Lys Asn Tyr

50 55 60

Ile Ser Arg Asn Leu Lys Ala Gln Ala Val Ile Trp

65 70 75

<210> 331

<211> 231

<212> DNA

<213> 乳房链球菌(Streptococcus uberis)

<400> 331

atggacattt tattagaact cgcaggatat actgggatag cctcaggtac tgcaaaaaaa 60

gttgttgatg ccattgataa aggagctgca gcctttgtta ttatttcaat tatctcaaca 120

gtaattagtg cgggagcatt gggagcagtt tcagcctcag ctgattttat tattttaact 180

gtaaaaaatt acattagtag aaatttaaaa gcacaagctg tcatttggta a 231

<210> 332

<211> 64

<212> PRT

<213> 产气荚膜梭菌(Clostridium perfringens)

<400> 332

Met Asp Ser Glu Leu Phe Lys Leu Met Ala Thr Gln Gly Ala Phe Ala

1 5 10 15

Ile Leu Phe Ser Tyr Leu Leu Phe Tyr Val Leu Lys Glu Asn Ser Lys

20 25 30

Arg Glu Asp Lys Tyr Gln Asn Ile Ile Glu Glu Leu Thr Glu Leu Leu

35 40 45

Pro Lys Ile Lys Glu Asp Val Glu Asp Ile Lys Glu Lys Leu Asn Lys

50 55 60

<210> 333

<211> 195

<212> DNA

<213> 产气荚膜梭菌(Clostridium perfringens)

<400> 333

atggatagtg aattatttaa gttaatggca acacaaggag cctttgcaat attattttcg 60

tatttattgt tttatgtttt aaaagagaat agtaaaagag aagataagta tcaaaatata 120

atagaggagc ttacagaatt attgccaaaa ataaaagaag atgtagaaga tataaaagaa 180

aaacttaata aatag 195

<210> 334

<211> 47

<212> PRT

<213> 变异微球菌(Micrococcus varians)

<400> 334

Met Thr Asn Ala Phe Gln Ala Leu Asp Glu Val Thr Asp Ala Glu Leu

1 5 10 15

Asp Ala Ile Leu Gly Gly Gly Ser Gly Val Ile Pro Thr Ile Ser His

20 25 30

Glu Cys His Met Asn Ser Phe Gln Phe Val Phe Thr Cys Cys Ser

35 40 45

<210> 335

<211> 144

<212> DNA

<213> 变异微球菌(Micrococcus varians)

<400> 335

atgacgaacg catttcaggc actggacgaa gtcacggacg ccgagctcga cgccatcctt 60

ggcgggggca gtggtgttat tcccacgatc agccacgagt gccacatgaa ctccttccag 120

ttcgtgttca cctgctgctc ctga 144

<210> 336

<211> 285

<212> PRT

<213> 马链球菌兽疫亚种(Streptococcus equi subsp. zooepidemicus)

<400> 336

Met Lys Arg Ile Phe Phe Ala Phe Leu Ser Leu Cys Leu Phe Ile Phe

1 5 10 15

Gly Thr Gln Thr Val Ser Ala Ala Thr Tyr Thr Arg Pro Leu Asp Thr

20 25 30

Gly Asn Ile Thr Thr Gly Phe Asn Gly Tyr Pro Gly His Val Gly Val

35 40 45

Asp Tyr Ala Val Pro Val Gly Thr Pro Val Arg Ala Val Ala Asn Gly

50 55 60

Thr Val Lys Phe Ala Gly Asn Gly Ala Asn His Pro Trp Met Leu Trp

65 70 75 80

Met Ala Gly Asn Cys Val Leu Ile Gln His Ala Asp Gly Met His Thr

85 90 95

Gly Tyr Ala His Leu Ser Lys Ile Ser Val Ser Thr Asp Ser Thr Val

100 105 110

Lys Gln Gly Gln Ile Ile Gly Tyr Thr Gly Ala Thr Gly Gln Val Thr

115 120 125

Gly Pro His Leu His Phe Glu Met Leu Pro Ala Asn Pro Asn Trp Gln

130 135 140

Asn Gly Phe Ser Gly Arg Ile Asp Pro Thr Gly Tyr Ile Ala Asn Ala

145 150 155 160

Pro Val Phe Asn Gly Thr Thr Pro Thr Glu Pro Thr Thr Pro Thr Thr

165 170 175

Asn Leu Lys Ile Tyr Lys Val Asp Asp Leu Gln Lys Ile Asn Gly Ile

180 185 190

Trp Gln Val Arg Asn Asn Ile Leu Val Pro Thr Asp Phe Thr Trp Val

195 200 205

Asp Asn Gly Ile Ala Ala Asp Asp Val Ile Glu Val Thr Ser Asn Gly

210 215 220

Thr Arg Thr Ser Asp Gln Val Leu Gln Lys Gly Gly Tyr Phe Val Ile

225 230 235 240

Asn Pro Asn Asn Val Lys Ser Val Gly Thr Pro Met Lys Gly Ser Gly

245 250 255

Gly Leu Ser Trp Ala Gln Val Asn Phe Thr Thr Gly Gly Asn Val Trp

260 265 270

Leu Asn Thr Thr Ser Lys Asp Asn Leu Leu Tyr Gly Lys

275 280 285

<210> 337

<211> 858

<212> DNA

<213> 马链球菌兽疫亚种(Streptococcus equi subsp. zooepidemicus)

<400> 337

atgaaacgta tattttttgc tttcttaagt ttatgcttat ttatattcgg aacacaaacg 60

gtatctgcag ctacttatac tcggccatta gatacgggaa atatcactac agggtttaac 120

ggataccctg gtcatgttgg agtcgattat gcagtacccg ttggaactcc ggttagagca 180

gttgcaaatg gtacagtcaa atttgcaggt aatggggcta atcacccatg gatgctttgg 240

atggctggaa actgtgttct aattcaacat gctgacggga tgcatactgg atatgcacac 300

ttatcaaaaa tttcagttag cacagatagt acagttaaac aaggacaaat cataggttat 360

actggtgcca ccggccaagt taccggtcca catttgcatt ttgaaatgtt gccagcaaat 420

cctaactggc aaaatggttt ttctggaaga atagatccaa ccggatacat cgctaatgcc 480

cctgtattta atggaacaac acctacagaa cctactactc ctacaacaaa tttaaaaatc 540

tataaagttg atgatttaca aaaaattaat ggtatttggc aagtaagaaa taacatactt 600

gtaccaactg atttcacatg ggttgataat ggaattgcag cagatgatgt aattgaagta 660

actagcaatg gaacaagaac ctctgaccaa gttcttcaaa aaggtggtta ttttgtcatc 720

aatcctaata atgttaaaag tgttggaact ccgatgaaag gtagtggtgg tctatcttgg 780

gctcaagtaa actttacaac aggtggaaat gtctggttaa atactactag caaagacaac 840

ttactttacg gaaaataa 858

<210> 338

<211> 45

<212> PRT

<213> 橙色粘球菌(Myxococcus fulvus)

<400> 338

Ala Asn Cys Ser Cys Ser Thr Ala Ser Asp Tyr Cys Pro Ile Leu Thr

1 5 10 15

Phe Cys Thr Thr Gly Thr Ala Cys Ser Tyr Thr Pro Thr Gly Cys Gly

20 25 30

Thr Gly Trp Val Tyr Cys Ala Cys Asn Gly Asn Phe Tyr

35 40 45

<210> 339

<211> 135

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO: 338的多肽的多核苷酸

<400> 339

gcgaactgca gctgcagcac cgcgagcgat tattgcccga ttctgacctt ttgcaccacc 60

ggcaccgcgt gcagctatac cccgaccggc tgcggcaccg gctgggtgta ttgcgcgtgc 120

aacggcaact tttat 135

<210> 340

<211> 19

<212> PRT

<213> 灰藤黄链霉菌(Streptomyces griseoluteus)

<400> 340

Cys Ala Asn Ser Cys Ser Tyr Gly Pro Leu Thr Trp Ser Cys Asp Gly

1 5 10 15

Asn Thr Lys

<210> 341

<211> 57

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO: 340的多肽的多核苷酸

<400> 341

tgcgcgaaca gctgcagcta tggcccgctg acctggagct gcgatggcaa caccaaa 57

<210> 342

<211> 19

<212> PRT

<213> 灰轮丝链轮丝菌(Streptoverticillium griseoverticillatum)

<400> 342

Cys Lys Gln Ser Cys Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly

1 5 10 15

Asn Thr Lys

<210> 343

<211> 57

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO: 342的多肽的多核苷酸

<400> 343

tgcaaacaga gctgcagctt tggcccgttt acctttgtgt gcgatggcaa caccaaa 57

<210> 344

<211> 7

<212> PRT

<213> 肉食杆菌(Carnobacterium sp.)

<400> 344

Gly Ser Glu Ile Gln Pro Arg

1 5

<210> 345

<211> 21

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:344的多肽的多核苷酸

<400> 345

ggcagcgaaa ttcagccgcg c 21

<210> 346

<211> 39

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 346

Gly Thr Trp Asp Asp Ile Gly Gln Gly Ile Gly Arg Val Ala Tyr Trp

1 5 10 15

Val Gly Lys Ala Met Gly Asn Met Ser Asp Val Asn Gln Ala Ser Arg

20 25 30

Ile Asn Arg Lys Lys Lys His

35

<210> 347

<211> 117

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:346的多肽的多核苷酸

<400> 347

ggcacctggg atgatattgg ccagggcatt ggccgcgtgg cgtattgggt gggcaaagcg 60

atgggcaaca tgagcgatgt gaaccaggcg agccgcatta accgcaaaaa aaaacat 117

<210> 348

<211> 35

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 348

Lys Lys Trp Gly Trp Leu Ala Trp Val Asp Pro Ala Tyr Glu Phe Ile

1 5 10 15

Lys Gly Phe Gly Lys Gly Ala Ile Lys Glu Gly Asn Lys Asp Lys Trp

20 25 30

Lys Asn Ile

35

<210> 349

<211> 105

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:348的多肽的多核苷酸

<400> 349

aaaaaatggg gctggctggc gtgggtggat ccggcgtatg aatttattaa aggctttggc 60

aaaggcgcga ttaaagaagg caacaaagat aaatggaaaa acatt 105

<210> 350

<211> 19

<212> PRT

<213> 链霉菌(Streptomyces sp.)

<400> 350

Cys Val Gln Ser Cys Ser Phe Gly Pro Leu Thr Trp Ser Cys Asp Gly

1 5 10 15

Asn Thr Lys

<210> 351

<211> 57

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:350的多肽的多核苷酸

<400> 351

tgcgtgcaga gctgcagctt tggcccgctg acctggagct gcgatggcaa caccaaa 57

<210> 352

<211> 19

<212> PRT

<213> 利古里亚游动放线菌(Actinoplanes liguriae)

<400> 352

Ser Ser Gly Trp Val Cys Thr Leu Thr Ile Glu Cys Gly Thr Val Ile

1 5 10 15

Cys Ala Cys

<210> 353

<211> 57

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:352的多肽的多核苷酸

<400> 353

agcagcggct gggtgtgcac cctgaccatt gaatgcggca ccgtgatttg cgcgtgc 57

<210> 354

<211> 38

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<220>

<221> VARIANT

<222> 34

<223> Xaa =任意氨基酸

<220>

<221> VARIANT

<222> 37

<223> Xaa =任意氨基酸

<400> 354

Tyr Thr Ala Lys Gln Cys Leu Gln Ala Ile Gly Ser Cys Gly Ile Ala

1 5 10 15

Gly Thr Gly Ala Gly Ala Ala Gly Gly Pro Ala Gly Ala Phe Val Gly

20 25 30

Ala Xaa Val Val Xaa Ile

35

<210> 355

<211> 114

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:354的多肽的多核苷酸

<220>

<221> misc_feature

<222> 100, 101, 102, 109, 110, 111

<223> n = A、T、C或G

<220>

<221> misc_feature

<222> (100)...(102)

<223> nnn = 除了终止密码子外的密码子

<220>

<221> misc_feature

<222> (109)...(111)

<223> nnn = 除了终止密码子外的密码子

<400> 355

tataccgcga aacagtgcct gcaggcgatt ggcagctgcg gcattgcggg caccggcgcg 60

ggcgcggcgg gcggcccggc gggcgcgttt gtgggcgcgn nngtggtgnn natt 114

<210> 356

<211> 42

<212> PRT

<213> 清酒乳杆菌(Lactobacillus sakei)

<220>

<221> VARIANT

<222> 32

<223> Xaa =任意氨基酸

<400> 356

Thr Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Asn Ser Lys Lys Cys Trp

1 5 10 15

Val Asp Trp Gly Gln Ala Ala Gly Gly Ile Gly Gln Thr Val Val Xaa

20 25 30

Gly Trp Leu Gly Gly Ala Ile Pro Gly Lys

35 40

<210> 357

<211> 126

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:356的多肽的多核苷酸

<220>

<221> misc_feature

<222> (94)...(96)

<223> nnn =编码任意氨基酸的三联体

<400> 357

accaaatatt atggcaacgg cgtgtattgc aacagcaaaa aatgctgggt ggattggggc 60

caggcggcgg gcggcattgg ccagaccgtg gtgnnnggct ggctgggcgg cgcgattccg 120

ggcaaa 126

<210> 358

<211> 22

<212> PRT

<213> 变异链球菌(Streptococcus mutans)

<400> 358

Phe Lys Ser Trp Ser Phe Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser

1 5 10 15

Phe Asn Ser Tyr Cys Cys

20

<210> 359

<211> 132

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:358的多肽的多核苷酸

<400> 359

tttaaaagct ggagcttttg caccccgggc tgcgcgaaaa ccggcagctt taacagctat 60

tgctgcttta aaagctggag cttttgcacc ccgggctgcg cgaaaaccgg cagctttaac 120

agctattgct gc 132

<210> 360

<211> 43

<212> PRT

<213> 蒙氏肠球菌(Enterococcus mundtii)

<400> 360

Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val

1 5 10 15

Asp Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala Asn

20 25 30

Leu Ala Thr Gly Gly Ala Ala Gly Trp Ser Lys

35 40

<210> 361

<211> 129

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:360的多肽的多核苷酸

<400> 361

aaatattatg gcaacggcgt gagctgcaac aaaaaaggct gcagcgtgga ttggggcaaa 60

gcgattggca ttattggcaa caacagcgcg gcgaacctgg cgaccggcgg cgcggcgggc 120

tggagcaaa 129

<210> 362

<211> 41

<212> PRT

<213> 清酒乳杆菌(Lactobacillus sakei)

<220>

<221> VARIANT

<222> 9, 14, 33, 37

<223> Xaa =任意氨基酸

<400> 362

Lys Tyr Tyr Gly Asn Gly Val His Xaa Gly Lys His Ser Xaa Thr Val

1 5 10 15

Asp Trp Gly Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala Ala Asn

20 25 30

Xaa Ala Thr Gly Xaa Asn Ala Gly Gly

35 40

<210> 363

<211> 123

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:362的多肽的多核苷酸

<220>

<221> misc_feature

<222> (25)...(27)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (40)...(42)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (97)...(99)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (109)...(111)

<223> nnn =编码任意氨基酸的三联体

<400> 363

aaatattatg gcaacggcgt gcatnnnggc aaacatagcn nnaccgtgga ttggggcacc 60

gcgattggca acattggcaa caacgcggcg gcgaacnnng cgaccggcnn naacgcgggc 120

ggc 123

<210> 364

<211> 31

<212> PRT

<213> 副干酪乳杆菌(Lactobacillus paracasei)

<400> 364

Gly Met Ser Gly Tyr Ile Gln Gly Ile Pro Asp Phe Leu Lys Gly Tyr

1 5 10 15

Leu His Gly Ile Ser Ala Ala Asn Lys His Lys Lys Gly Arg Leu

20 25 30

<210> 365

<211> 93

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:364的多肽的多核苷酸

<400> 365

ggcatgagcg gctatattca gggcattccg gattttctga aaggctatct gcatggcatt 60

agcgcggcga acaaacataa aaaaggccgc ctg 93

<210> 366

<211> 31

<212> PRT

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 366

Lys Gly Lys Gly Phe Trp Ser Trp Ala Ser Lys Ala Thr Ser Trp Leu

1 5 10 15

Thr Gly Pro Gln Gln Pro Gly Ser Pro Leu Leu Lys Lys His Arg

20 25 30

<210> 367

<211> 93

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:366的多肽的多核苷酸

<400> 367

aaaggcaaag gcttttggag ctgggcgagc aaagcgacca gctggctgac cggcccgcag 60

cagccgggca gcccgctgct gaaaaaacat cgc 93

<210> 368

<211> 43

<212> PRT

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 368

Lys Asn Tyr Gly Asn Gly Val His Cys Thr Lys Lys Gly Cys Ser Val

1 5 10 15

Asp Trp Gly Tyr Ala Trp Thr Asn Ile Ala Asn Asn Ser Val Met Asn

20 25 30

Gly Leu Thr Gly Gly Asn Ala Gly Trp His Asn

35 40

<210> 369

<211> 129

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:368的多肽的多核苷酸

<400> 369

aaaaactatg gcaacggcgt gcattgcacc aaaaaaggct gcagcgtgga ttggggctat 60

gcgtggacca acattgcgaa caacagcgtg atgaacggcc tgaccggcgg caacgcgggc 120

tggcataac 129

<210> 370

<211> 47

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 370

Ala Ile Lys Leu Val Gln Ser Pro Asn Gly Asn Phe Ala Ala Ser Phe

1 5 10 15

Val Leu Asp Gly Thr Lys Trp Ile Phe Lys Ser Lys Tyr Tyr Asp Ser

20 25 30

Ser Lys Gly Tyr Trp Val Gly Ile Tyr Glu Val Trp Asp Arg Lys

35 40 45

<210> 371

<211> 141

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:370的多肽的多核苷酸

<400> 371

gcgattaaac tggtgcagag cccgaacggc aactttgcgg cgagctttgt gctggatggc 60

accaaatgga tttttaaaag caaatattat gatagcagca aaggctattg ggtgggcatt 120

tatgaagtgt gggatcgcaa a 141

<210> 372

<211> 12

<212> PRT

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<220>

<221> VARIANT

<222> 7

<223> Xaa =任意氨基酸

<400> 372

Ile Ser Leu Glu Ile Cys Xaa Ile Phe His Asp Asn

1 5 10

<210> 373

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:372的多肽的多核苷酸

<220>

<221> misc_feature

<222> (19)...(21)

<223> nnn =编码任意氨基酸的三联体

<400> 373

attagcctgg aaatttgcnn natttttcat gataac 36

<210> 374

<211> 37

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 374

Thr Ser Tyr Gly Asn Gly Val His Cys Asn Lys Ser Lys Cys Trp Ile

1 5 10 15

Asp Val Ser Glu Leu Glu Thr Tyr Lys Ala Gly Thr Val Ser Asn Pro

20 25 30

Lys Asp Ile Leu Trp

35

<210> 375

<211> 111

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:374的多肽的多核苷酸

<400> 375

accagctatg gcaacggcgt gcattgcaac aaaagcaaat gctggattga tgtgagcgaa 60

ctggaaacct ataaagcggg caccgtgagc aacccgaaag atattctgtg g 111

<210> 376

<211> 9

<212> PRT

<213> 普城沙雷菌(Serratia plymuthica)

<400> 376

Asp Tyr His His Gly Val Arg Val Leu

1 5

<210> 377

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:376的多肽的多核苷酸

<400> 377

gattatcatc atggcgtgcg cgtgctg 27

<210> 378

<211> 15

<212> PRT

<213> 盐杆菌(Halobacterium sp.)

<400> 378

Asp Ile Asp Ile Thr Gly Cys Ser Ala Cys Lys Tyr Ala Ala Gly

1 5 10 15

<210> 379

<211> 45

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:378的多肽的多核苷酸

<400> 379

gatattgata ttaccggctg cagcgcgtgc aaatatgcgg cgggc 45

<210> 380

<211> 12

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<220>

<221> VARIANT

<222> 1, 2, 6

<223> Xaa =任意氨基酸

<400> 380

Xaa Xaa Lys Glu Ile Xaa His Ile Phe His Asp Asn

1 5 10

<210> 381

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:380的多肽的多核苷酸

<220>

<221> misc_feature

<222> (1)...(3)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (4)...(6)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (16)...(18)

<223> nnn =编码任意氨基酸的三联体

<400> 381

nnnnnnaaag aaattnnnca tatttttcat gataac 36

<210> 382

<211> 12

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 382

Thr Pro Val Val Asn Pro Pro Phe Leu Gln Gln Thr

1 5 10

<210> 383

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:382的多肽的多核苷酸

<400> 383

accccggtgg tgaacccgcc gtttctgcag cagacc 36

<210> 384

<211> 10

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<220>

<221> VARIANT

<222> 10

<223> Xaa =任意氨基酸

<400> 384

Val Ala Pro Phe Pro Glu Gln Phe Leu Xaa

1 5 10

<210> 385

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:384的多肽的多核苷酸

<220>

<221> misc_feature

<222> (28)...(30)

<223> nnn =编码任意氨基酸的三联体

<400> 385

gtggcgccgt ttccggaaca gtttctgnnn 30

<210> 386

<211> 9

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 386

Asn Ile Pro Gln Leu Thr Pro Thr Pro

1 5

<210> 387

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:386的多肽的多核苷酸

<400> 387

aacattccgc agctgacccc gaccccg 27

<210> 388

<211> 18

<212> PRT

<213> 苏云金芽孢杆菌(Bacillus thuringiensis) subsp. entomocidus

<220>

<221> VARIANT

<222> 4, 7, 10

<223> Xaa =任意氨基酸

<400> 388

Asp Trp Thr Xaa Trp Ser Xaa Leu Val Xaa Ala Ala Cys Ser Val Glu

1 5 10 15

Leu Leu

<210> 389

<211> 54

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:388的多肽的多核苷酸

<220>

<221> misc_feature

<222> (10)...(12)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (19)...(21)

<223> nnn =编码任意氨基酸的三联体

<220>

<221> misc_feature

<222> (28)...(30)

<223> nnn =编码任意氨基酸的三联体

<400> 389

gattggaccn nntggagcnn nctggtgnnn gcggcgtgca gcgtggaact gctg 54

<210> 390

<211> 30

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 390

Ala Tyr Pro Gly Asn Gly Val His Cys Gly Lys Tyr Ser Cys Thr Val

1 5 10 15

Asp Lys Gln Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala

20 25 30

<210> 391

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:390的多肽的多核苷酸

<400> 391

gcgtatccgg gcaacggcgt gcattgcggc aaatatagct gcaccgtgga taaacagacc 60

gcgattggca acattggcaa caacgcggcg 90

<210> 392

<211> 43

<212> PRT

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 392

Thr Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Asn Ser Lys Lys Cys Trp

1 5 10 15

Val Asp Trp Gly Thr Ala Gln Gly Cys Ile Asp Val Val Ile Gly Gln

20 25 30

Leu Gly Gly Gly Ile Pro Gly Lys Gly Lys Cys

35 40

<210> 393

<211> 129

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:392的多肽的多核苷酸

<400> 393

accaaatatt atggcaacgg cgtgtattgc aacagcaaaa aatgctgggt ggattggggc 60

accgcgcagg gctgcattga tgtggtgatt ggccagctgg gcggcggcat tccgggcaaa 120

ggcaaatgc 129

<210> 394

<211> 62

<212> PRT

<213> 肠球菌(Enterococcus sp.)

<400> 394

Asn Arg Trp Tyr Cys Asn Ser Ala Ala Gly Gly Val Gly Gly Ala Ala

1 5 10 15

Val Cys Gly Leu Ala Gly Tyr Val Gly Glu Ala Lys Glu Asn Ile Ala

20 25 30

Gly Glu Val Arg Lys Gly Trp Gly Met Ala Gly Gly Phe Thr His Asn

35 40 45

Lys Ala Cys Lys Ser Phe Pro Gly Ser Gly Trp Ala Ser Gly

50 55 60

<210> 395

<211> 186

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:394的多肽的多核苷酸

<400> 395

aaccgctggt attgcaacag cgcggcgggc ggcgtgggcg gcgcggcggt gtgcggcctg 60

gcgggctatg tgggcgaagc gaaagaaaac attgcgggcg aagtgcgcaa aggctggggc 120

atggcgggcg gctttaccca taacaaagcg tgcaaaagct ttccgggcag cggctgggcg 180

agcggc 186

<210> 396

<211> 39

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 396

Thr Thr Lys Asn Tyr Gly Asn Gly Val Cys Asn Ser Val Asn Trp Cys

1 5 10 15

Gln Cys Gly Asn Val Trp Ala Ser Cys Asn Leu Ala Thr Gly Cys Ala

20 25 30

Ala Trp Leu Cys Lys Leu Ala

35

<210> 397

<211> 117

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:396的多肽的多核苷酸

<400> 397

accaccaaaa actatggcaa cggcgtgtgc aacagcgtga actggtgcca gtgcggcaac 60

gtgtgggcga gctgcaacct ggcgaccggc tgcgcggcgt ggctgtgcaa actggcg 117

<210> 398

<211> 30

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 398

Ala Ser Ile Ile Lys Thr Thr Ile Lys Val Ser Lys Ala Val Cys Lys

1 5 10 15

Thr Leu Thr Cys Ile Cys Thr Gly Ser Cys Ser Asn Cys Lys

20 25 30

<210> 399

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:398的多肽的多核苷酸

<400> 399

gcgagcatta ttaaaaccac cattaaagtg agcaaagcgg tgtgcaaaac cctgacctgc 60

atttgcaccg gcagctgcag caactgcaaa 90

<210> 400

<211> 31

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 400

Ser Ala Ser Ile Val Lys Thr Thr Ile Lys Ala Ser Lys Lys Leu Cys

1 5 10 15

Arg Gly Phe Thr Leu Thr Cys Gly Cys His Phe Thr Gly Lys Lys

20 25 30

<210> 401

<211> 93

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:400的多肽的多核苷酸

<400> 401

agcgcgagca ttgtgaaaac caccattaaa gcgagcaaaa aactgtgccg cggctttacc 60

ctgacctgcg gctgccattt taccggcaaa aaa 93

<210> 402

<211> 43

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 402

Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val

1 5 10 15

Asp Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ala Ala Ala Asn

20 25 30

Leu Thr Thr Gly Gly Lys Ala Ala Trp Ala Cys

35 40

<210> 403

<211> 129

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:402的多肽的多核苷酸

<400> 403

aaatattatg gcaacggcgt gagctgcaac aaaaaaggct gcagcgtgga ttggggcaaa 60

gcgattggca ttattggcaa caacgcggcg gcgaacctga ccaccggcgg caaagcggcg 120

tgggcgtgc 129

<210> 404

<211> 39

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 404

Ala Thr Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Gln Lys His Tyr

1 5 10 15

Thr Trp Val Asp Trp Asn Lys Ala Ser Arg Glu Ile Gly Lys Ile Thr

20 25 30

Val Asn Gly Trp Val Gln His

35

<210> 405

<211> 117

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:404的多肽的多核苷酸

<400> 405

gcgacctatt atggcaacgg cctgtattgc aacaaacaga aacattatac ctgggtggat 60

tggaacaaag cgagccgcga aattggcaaa attaccgtga acggctgggt gcagcat 117

<210> 406

<211> 35

<212> PRT

<213> 环状芽孢杆菌(Bacillus circulans)

<400> 406

Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys Ser Val

1 5 10 15

Asn Trp Gly Ile Ile Thr His Gln Ala Phe Arg Val Thr Ser Gly Val

20 25 30

Ala Ser Gly

35

<210> 407

<211> 105

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:406的多肽的多核苷酸

<400> 407

gtgaactatg gcaacggcgt gagctgcagc aaaaccaaat gcagcgtgaa ctggggcatt 60

attacccatc aggcgtttcg cgtgaccagc ggcgtggcga gcggc 105

<210> 408

<211> 30

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 408

Phe Val Tyr Gly Asn Gly Val Thr Ser Ile Leu Val Gln Ala Gln Phe

1 5 10 15

Leu Val Asn Gly Gln Arg Arg Phe Phe Tyr Thr Pro Asp Lys

20 25 30

<210> 409

<211> 90

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:408的多肽的多核苷酸

<400> 409

tttgtgtatg gcaacggcgt gaccagcatt ctggtgcagg cgcagtttct ggtgaacggc 60

cagcgccgct ttttttatac cccggataaa 90

<210> 410

<211> 13

<212> PRT

<213> 鼠李糖乳杆菌(Lactobacillus rhamnosus)

<400> 410

Ala Val Pro Ala Val Arg Lys Thr Asn Glu Thr Leu Asp

1 5 10

<210> 411

<211> 39

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:410的多肽的多核苷酸

<400> 411

gcggtgccgg cggtgcgcaa aaccaacgaa accctggat 39

<210> 412

<211> 69

<212> PRT

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<400> 412

Met Lys Asn Ser Ala Ala Arg Glu Ala Phe Lys Gly Ala Asn His Pro

1 5 10 15

Ala Gly Met Val Ser Glu Glu Glu Leu Lys Ala Leu Val Gly Gly Asn

20 25 30

Asp Val Asn Pro Glu Thr Thr Pro Ala Thr Thr Ser Ser Trp Thr Cys

35 40 45

Ile Thr Ala Gly Val Thr Val Ser Ala Ser Leu Cys Pro Thr Thr Lys

50 55 60

Cys Thr Ser Arg Cys

65

<210> 413

<211> 207

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:412的多肽的多核苷酸

<400> 413

atgaaaaaca gcgcggcgcg cgaagcgttt aaaggcgcga accatccggc gggcatggtg 60

agcgaagaag aactgaaagc gctggtgggc ggcaacgatg tgaacccgga aaccaccccg 120

gcgaccacca gcagctggac ctgcattacc gcgggcgtga ccgtgagcgc gagcctgtgc 180

ccgaccacca aatgcaccag ccgctgc 207

<210> 414

<211> 36

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 414

Lys Tyr Tyr Gly Asn Gly Leu Ser Cys Ser Lys Lys Gly Cys Thr Val

1 5 10 15

Asn Trp Gly Gln Ala Phe Ser Cys Gly Val Asn Arg Val Ala Thr Ala

20 25 30

Gly His Gly Lys

35

<210> 415

<211> 108

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:414的多肽的多核苷酸

<400> 415

aaatattatg gcaacggcct gagctgcagc aaaaaaggct gcaccgtgaa ctggggccag 60

gcgtttagct gcggcgtgaa ccgcgtggcg accgcgggcc atggcaaa 108

<210> 416

<211> 24

<212> PRT

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 416

Gly Asn Pro Lys Val Ala His Cys Ala Ser Gln Ile Gly Arg Ser Thr

1 5 10 15

Ala Trp Gly Ala Val Ser Gly Ala

20

<210> 417

<211> 72

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:416的多肽的多核苷酸

<400> 417

ggcaacccga aagtggcgca ttgcgcgagc cagattggcc gcagcaccgc gtggggcgcg 60

gtgagcggcg cg 72

<210> 418

<211> 40

<212> PRT

<213> 粪肠球菌(Enterococcus faecalis)

<400> 418

Trp Leu Pro Pro Ala Gly Leu Leu Gly Arg Cys Gly Arg Trp Phe Arg

1 5 10 15

Pro Trp Leu Leu Trp Leu Gln Ser Gly Ala Gln Tyr Lys Trp Leu Gly

20 25 30

Asn Leu Phe Gly Leu Gly Pro Lys

35 40

<210> 419

<211> 120

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 编码SEQ ID NO:418的多肽的多核苷酸

<400> 419

tggctgccgc cggcgggcct gctgggccgc tgcggccgct ggtttcgccc gtggctgctg 60

tggctgcaga gcggcgcgca gtataaatgg ctgggcaacc tgtttggcct gggcccgaaa 120

<210> 420

<211> 40

<212> PRT

<213> 多变鱼腥藻(Anabaena variabilis)

<400> 420

Asn Leu Asp Gln Trp Leu Thr Glu Gln Val His Glu Phe Gln Asp Met

1 5 10 15

Tyr Leu Glu Pro Gln Ala Ile Ser Asn Gln Asp Ile Thr Phe Lys Leu

20 25 30

Ser Asp Leu Asp Phe Ile His Asn

35 40

<210> 421

<211> 124

<212> DNA

<213> 多变鱼腥藻(Anabaena variabilis)

<400> 421

taatttagat cagtggttaa cagaacaagt tcatgagttt caagatatgt acttggaacc 60

acaagcaata tccaatcaag acattacctt caaactatct gacctagatt ttattcataa 120

ttga 124

<210> 422

<211> 40

<212> PRT

<213> 念珠藻(Nostoc sp)

<400> 422

Asn Leu Asp Gln Trp Leu Thr Glu Gln Val His Glu Phe Gln Asp Met

1 5 10 15

Tyr Leu Glu Pro Gln Ala Ile Ser Asn Gln Asp Ile Thr Phe Lys Leu

20 25 30

Ser Asp Leu Asp Phe Ile His Asn

35 40

<210> 423

<211> 123

<212> DNA

<213> 念珠藻(Nostoc sp)

<400> 423

aatttagatc aatggttaac agaacaagtt catgagtttc aagatatgta cttggaacca 60

caagcaatat ccaatcaaga cattaccttc aaactgtcag acctagattt tattcataat 120

tga 123

<210> 424

<211> 7

<212> PRT

<213> 满江红念珠藻(Nostoc azollae)

<400> 424

His Arg Glu Lys Lys Ser Ala

1 5

<210> 425

<211> 24

<212> DNA

<213> 满江红念珠藻(Nostoc azollae)

<400> 425

cacagagaga aaaaatcagc atag 24

<210> 426

<211> 23

<212> PRT

<213> Acaryochloris marina

<400> 426

Thr Ser Asn Asn Trp Leu Ala Lys Asn Tyr Leu Ser Met Trp Asn Lys

1 5 10 15

Lys Ser Ser Asn Pro Asn Leu

20

<210> 427

<211> 72

<212> DNA

<213> Acaryochloris marina

<400> 427

acaagcaata actggctagc caaaaactat ctttctatgt ggaataaaaa gagcagtaat 60

ccaaaccttt ag 72

<210> 428

<211> 6

<212> PRT

<213> 蓝杆藻(Cyanothece)

<400> 428

Phe Arg Tyr Phe Trp Trp

1 5

<210> 429

<211> 21

<212> DNA

<213> 蓝杆藻(Cyanothece)

<400> 429

tttagatatt tttggtggta a 21

<210> 430

<211> 6

<212> PRT

<213> 蓝杆藻(Cyanothece)

<400> 430

Phe Arg Tyr Phe Trp Trp

1 5

<210> 431

<211> 21

<212> DNA

<213> 蓝杆藻(Cyanothece)

<400> 431

tttagatatt tttggtggta a 21

<210> 432

<211> 9

<212> PRT

<213> 蓝杆藻(Cyanothece)

<400> 432

Cys Gly Glu Lys Trp Arg Ile Phe Ser

1 5

<210> 433

<211> 27

<212> DNA

<213> 蓝杆藻(Cyanothece)

<400> 433

tgtggagaaa aatggagaat ttttagc 27

<210> 434

<211> 8

<212> PRT

<213> 蓝杆藻(Cyanothece)

<400> 434

Phe Arg Leu Gln Leu Trp Gln Phe

1 5

<210> 435

<211> 24

<212> DNA

<213> 蓝杆藻(Cyanothece)

<400> 435

tttcgcttac aactgtggca attt 24

<210> 436

<211> 16

<212> PRT

<213> 蓝杆藻(Cyanothece)

<400> 436

Leu Gly Cys Asn Gln Ser Ser Ile Trp Ser Ile Phe Phe Trp Asn His

1 5 10 15

<210> 437

<211> 51

<212> DNA

<213> 蓝杆藻(Cyanothece)

<400> 437

ctaggatgta accagagcag tatctggtca atttttttct ggaatcatta a 51

<210> 438

<211> 40

<212> PRT

<213> 原型微鞘藻(Microcoleus chthonoplastes)

<400> 438

Tyr Asn Leu Gln Gly Leu Pro Ala Ile Glu Ser Glu Asp Cys Ile Pro

1 5 10 15

Asp Ser Val Ala Pro Ser Asp Asp Trp Phe Ser Gly Val Ser Ser Leu

20 25 30

Phe Asn Arg Leu Thr Gly Leu Gly

35 40

<210> 439

<211> 123

<212> DNA

<213> 原型微鞘藻(Microcoleus chthonoplastes)

<400> 439

tataacctac aggggttgcc agcaattgag tcagaagact gtatcccaga ttctgtagcg 60

ccttcggatg attggttttc aggcgtatcg tctctgttta accgcttgac tgggttgggt 120

tag 123

<210> 440

<211> 37

<212> PRT

<213> 念珠藻(Nostoc sp)

<400> 440

Trp Met Ala Ile Arg Arg Ile Leu Arg Cys His Pro Phe His Pro Gly

1 5 10 15

Gly Tyr Asp Pro Val Pro Glu Leu Gly Glu His Cys Cys His His Asp

20 25 30

Ser Gly Asn Lys Gly

35

<210> 441

<211> 114

<212> DNA

<213> 念珠藻(Nostoc sp)

<400> 441

tggatggcga ttcgccgcat tttgcgttgt catccattcc acccaggggg ttatgatcct 60

gtaccagagt tgggtgagca ttgttgtcat catgatagcg ggaataaggg gtga 114

<210> 442

<211> 35

<212> PRT

<213> 多变鱼腥藻(Anabaena variabilis)

<400> 442

Trp Met Gly Ile Arg Arg Ile Leu Arg Cys His Pro Phe His Pro Gly

1 5 10 15

Gly Tyr Asp Pro Val Pro Glu Val Gly Glu His Cys Cys His His Asp

20 25 30

Ser Gly Lys

35

<210> 443

<211> 108

<212> DNA

<213> 多变鱼腥藻(Anabaena variabilis)

<400> 443

tggatgggga ttcgccgcat tttgcgttgt catccattcc acccaggcgg ttatgatcct 60

gtaccagagg tgggtgagca ttgttgtcat catgatagcg ggaagtag 108

<210> 444

<211> 48

<212> PRT

<213> 泡沫节球藻(Nodularia spumigena)

<400> 444

Trp Met Ala Thr Arg Arg Ile Leu Arg Cys His Pro Phe His Pro Gly

1 5 10 15

Gly Tyr Asp Pro Val Pro Glu Val Lys His Asn Cys Cys Asp Gln His

20 25 30

Leu Ser Asp Ser Gly Lys Gln Thr Thr Glu Asp His His Lys Gly Ser

35 40 45

<210> 445

<211> 147

<212> DNA

<213> 泡沫节球藻(Nodularia spumigena)

<400> 445

tggatggcga ctcggcggat tttgcgttgt catcccttcc atcctggtgg atatgatcca 60

gttccagagg taaaacacaa ttgctgcgat cagcatctgt ccgattctgg gaaacagacc 120

acagaagacc atcacaaagg ctcgtag 147

<210> 446

<211> 34

<212> PRT

<213> 满江红念珠藻(Nostoc azollae)

<400> 446

Trp Met Ala Thr Leu Arg Ile Leu Arg Cys His Pro Phe His Pro Gly

1 5 10 15

Gly Tyr Asp Pro Val Pro Gly Leu Ala Glu Lys Ser Cys Cys Asp His

20 25 30

His Asp

<210> 447

<211> 105

<212> DNA

<213> 满江红念珠藻(Nostoc azollae)

<400> 447

tggatggcaa ctttgcggat tttacgctgt catcctttcc atcctggtgg ttatgatcct 60

gtaccaggac tagcggaaaa atcctgttgt gaccatcatg attga 105

<210> 448

<211> 28

<212> PRT

<213> 聚球藻(Synechococcus)

<400> 448

Trp Leu Thr Ala Lys Arg Phe Cys Arg Cys His Pro Leu His Pro Gly

1 5 10 15

Gly Tyr Asp Pro Val Pro Glu Lys Lys Ser Val Leu

20 25

<210> 449

<211> 87

<212> DNA

<213> 聚球藻(Synechococcus)

<400> 449

tggctaacag ccaagcgctt ttgtcgctgt catccgcttc atcctggcgg gtatgatccg 60

gtaccggaga agaaatcggt actctaa 87

<210> 450

<211> 23

<212> PRT

<213> 海洋原绿球菌(Prochlorococcus marinus)

<400> 450

Trp Leu Thr Leu Arg Arg Leu Ser Arg Cys His Pro Phe Thr Pro Cys

1 5 10 15

Gly Cys Asp Pro Val Pro Asp

20

<210> 451

<211> 72

<212> DNA

<213> 海洋原绿球菌(Prochlorococcus marinus)

<400> 451

tggctcaccc tgcggcgcct gtctcgttgc catcctttta ccccctgtgg ttgcgacccg 60

gtgcctgatt aa 72

<210> 452

<211> 69

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 452

Met Ser Tyr Lys Lys Leu Tyr Gln Leu Thr Ala Ile Phe Ser Leu Pro

1 5 10 15

Leu Thr Ile Leu Leu Val Ser Leu Ser Ser Leu Arg Ile Val Gly Glu

20 25 30

Gly Asn Ser Tyr Val Asp Val Phe Leu Ser Phe Ile Ile Phe Leu Gly

35 40 45

Phe Ile Glu Leu Ile His Gly Ile Arg Lys Ile Leu Val Trp Ser Gly

50 55 60

Trp Lys Asn Gly Ser

65

<210> 453

<211> 210

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 453

atgagttata aaaaactgta ccaattgacg gctatattta gtttacctct tactatctta 60

ttggtttcac tttcatccct tcggattgtt ggcgaaggga attcttatgt tgacgttttt 120

ctaagcttta taatatttct tggttttatt gagctgattc atgggattcg aaagattttg 180

gtctggtcag gctggaaaaa cggaagttaa 210

<210> 454

<211> 85

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 454

Met Gly Leu Lys Leu Asp Leu Thr Trp Phe Asp Lys Ser Thr Glu Asp

1 5 10 15

Phe Lys Gly Glu Glu Tyr Ser Lys Asp Phe Gly Asp Asp Gly Ser Val

20 25 30

Met Glu Ser Leu Gly Val Pro Phe Lys Asp Asn Val Asn Asn Gly Cys

35 40 45

Phe Asp Val Ile Ala Glu Trp Val Pro Leu Leu Gln Pro Tyr Phe Asn

50 55 60

His Gln Ile Asp Ile Ser Asp Asn Glu Tyr Phe Val Ser Phe Asp Tyr

65 70 75 80

Arg Asp Gly Asp Trp

85

<210> 455

<211> 258

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 455

atgggactta aattggattt aacttggttt gataaaagta cagaagattt taagggtgag 60

gagtattcaa aagattttgg agatgacggt tcagttatgg aaagtctagg tgtgcctttt 120

aaggataatg ttaataacgg ttgctttgat gttatagctg aatgggtacc tttgctacaa 180

ccatacttta atcatcaaat tgatatttcc gataatgagt attttgtttc gtttgattat 240

cgtgatggtg attggtga 258

<210> 456

<211> 113

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 456

Met Ser Leu Arg Tyr Tyr Ile Lys Asn Ile Leu Phe Gly Leu Tyr Cys

1 5 10 15

Thr Leu Ile Tyr Ile Tyr Leu Ile Thr Lys Asn Ser Glu Gly Tyr Tyr

20 25 30

Phe Leu Val Ser Asp Lys Met Leu Tyr Ala Ile Val Ile Ser Thr Ile

35 40 45

Leu Cys Pro Tyr Ser Lys Tyr Ala Ile Glu Tyr Ile Ala Phe Asn Phe

50 55 60

Ile Lys Lys Asp Phe Phe Glu Arg Arg Lys Asn Leu Asn Asn Ala Pro

65 70 75 80

Val Ala Lys Leu Asn Leu Phe Met Leu Tyr Asn Leu Leu Cys Leu Val

85 90 95

Leu Ala Ile Pro Phe Gly Leu Leu Gly Leu Phe Ile Ser Ile Lys Asn

100 105 110

Asn

<210> 457

<211> 342

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 457

atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac acttatatat 60

atatacctta taacaaaaaa cagcgaaggg tattatttcc ttgtgtcaga taagatgcta 120

tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat tgaatacata 180

gcttttaact tcataaagaa agattttttc gaaagaagaa aaaacctaaa taacgccccc 240

gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct agcaatccca 300

tttggattgc taggactttt tatatcaata aagaataatt aa 342

<210> 458

<211> 85

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 458

Met Gly Leu Lys Leu His Ile His Trp Phe Asp Lys Lys Thr Glu Glu

1 5 10 15

Phe Lys Gly Gly Glu Tyr Ser Lys Asp Phe Gly Asp Asp Gly Ser Val

20 25 30

Ile Glu Ser Leu Gly Met Pro Leu Lys Asp Asn Ile Asn Asn Gly Trp

35 40 45

Phe Asp Val Glu Lys Pro Trp Val Ser Ile Leu Gln Pro His Phe Lys

50 55 60

Asn Val Ile Asp Ile Ser Lys Phe Asp Tyr Phe Val Ser Phe Val Tyr

65 70 75 80

Arg Asp Gly Asn Trp

85

<210> 459

<211> 258

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 459

atggggctta aattacatat tcattggttt gataagaaaa ccgaagagtt taaaggcggt 60

gaatactcaa aagacttcgg tgatgatggt tctgtcattg aaagtctggg gatgccttta 120

aaggataata ttaataatgg ttggtttgat gttgaaaaac catgggtttc gatattacag 180

ccacacttta aaaatgtaat cgatattagt aaatttgatt actttgtatc ctttgtttac 240

cgggatggta actggtaa 258

<210> 460

<211> 86

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 460

Met Glu Leu Lys His Ser Ile Ser Asp Tyr Thr Glu Ala Glu Phe Leu

1 5 10 15

Glu Phe Val Lys Lys Ile Cys Arg Ala Glu Gly Ala Thr Glu Glu Asp

20 25 30

Asp Asn Lys Leu Val Arg Glu Phe Glu Arg Leu Thr Glu His Pro Asp

35 40 45

Gly Ser Asp Leu Ile Tyr Tyr Pro Arg Asp Asp Arg Glu Asp Ser Pro

50 55 60

Glu Gly Ile Val Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly Lys

65 70 75 80

Ser Gly Phe Lys Gln Gly

85

<210> 461

<211> 261

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 461

atggaactga aacatagtat tagtgattat accgaggctg aatttctgga gtttgtaaaa 60

aaaatatgta gagctgaagg tgctactgaa gaggatgaca ataaattagt gagagagttt 120

gagcgattaa ctgagcaccc agatggttca gatctgattt attatcctcg cgatgacagg 180

gaagatagtc ctgaagggat tgtcaaggaa attaaagaat ggcgagctgc taacggtaag 240

tcaggattta aacagggctg a 261

<210> 462

<211> 178

<212> PRT

<213> 弗氏柠檬酸杆菌(Citrobacter freundii)

<400> 462

Met Met Asn Glu His Ser Ile Asp Thr Asp Asn Arg Lys Ala Asn Asn

1 5 10 15

Ala Leu Tyr Leu Phe Ile Ile Ile Gly Leu Ile Pro Leu Leu Cys Ile

20 25 30

Phe Val Val Tyr Tyr Lys Thr Pro Asp Ala Leu Leu Leu Arg Lys Ile

35 40 45

Ala Thr Ser Thr Glu Asn Leu Pro Ser Ile Thr Ser Ser Tyr Asn Pro

50 55 60

Leu Met Thr Lys Val Met Asp Ile Tyr Cys Lys Thr Ala Pro Phe Leu

65 70 75 80

Ala Leu Ile Leu Tyr Ile Leu Thr Phe Lys Ile Arg Lys Leu Ile Asn

85 90 95

Asn Thr Asp Arg Asn Thr Val Leu Arg Ser Cys Leu Leu Ser Pro Leu

100 105 110

Val Tyr Ala Ala Ile Val Tyr Leu Phe Cys Phe Arg Asn Phe Glu Leu

115 120 125

Thr Thr Ala Gly Arg Pro Val Arg Leu Met Ala Thr Asn Asp Ala Thr

130 135 140

Leu Leu Leu Phe Tyr Ile Gly Leu Tyr Ser Ile Ile Phe Phe Thr Thr

145 150 155 160

Tyr Ile Thr Leu Phe Thr Pro Val Thr Ala Phe Lys Leu Leu Lys Lys

165 170 175

Arg Gln

<210> 463

<211> 537

<212> DNA

<213> 弗氏柠檬酸杆菌(Citrobacter freundii)

<400> 463

atgatgaatg aacactcaat agatacggac aacagaaagg ccaataacgc attgtattta 60

tttataataa tcggattaat accattattg tgcatttttg ttgtttacta caaaacgcca 120

gacgctttac ttttacgtaa aattgctaca agcactgaga atctcccgtc aataacatcc 180

tcctacaacc cattaatgac aaaggttatg gatatttatt gtaaaacagc gcctttcctt 240

gccttaatac tatacatcct aacctttaaa atcagaaaat taatcaacaa caccgacagg 300

aacactgtac ttagatcttg tttattaagt ccattggtct atgcagcaat tgtttatcta 360

ttctgcttcc gaaattttga gttaacaaca gccggaaggc ctgtcagatt aatggccacc 420

aatgacgcaa cactattgtt attttatatt ggtctgtact caataatttt ctttacaacc 480

tatatcacgc tattcacacc agtcactgca tttaaattat taaaaaaaag gcagtaa 537

<210> 464

<211> 111

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 464

Met Asn Arg Lys Tyr Tyr Phe Asn Asn Met Trp Trp Gly Trp Val Thr

1 5 10 15

Gly Gly Tyr Met Leu Tyr Met Ser Trp Asp Tyr Glu Phe Lys Tyr Arg

20 25 30

Leu Leu Phe Trp Cys Ile Ser Leu Cys Gly Met Val Leu Tyr Pro Val

35 40 45

Ala Lys Trp Tyr Ile Glu Asp Thr Ala Leu Lys Phe Thr Arg Pro Asp

50 55 60

Phe Trp Asn Ser Gly Phe Phe Ala Asp Thr Pro Gly Lys Met Gly Leu

65 70 75 80

Leu Ala Val Tyr Thr Gly Thr Val Phe Ile Leu Ser Leu Pro Leu Ser

85 90 95

Met Ile Tyr Ile Leu Ser Val Ile Ile Lys Arg Leu Ser Val Arg

100 105 110

<210> 465

<211> 336

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 465

atgaacagaa aatattattt taataatatg tggtggggat gggtgacggg gggatatatg 60

ctgtatatgt catgggatta tgagtttaaa tacagattac tgttctggtg tatttctctc 120

tgcggaatgg ttttgtatcc ggttgcaaaa tggtatattg aagatacagc tctaaaattt 180

acccggcctg atttctggaa cagcggtttt tttgctgata cacctggaaa aatggggttg 240

cttgcggttt atacgggtac tgttttcata ttatctcttc cgttaagtat gatatatatt 300

ctttctgtta ttataaaaag gctgtctgta agatag 336

<210> 466

<211> 115

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 466

Met Lys Leu Asp Ile Ser Val Lys Tyr Leu Leu Lys Ser Leu Ile Pro

1 5 10 15

Ile Leu Ile Ile Leu Thr Val Phe Tyr Leu Gly Trp Lys Asp Asn Gln

20 25 30

Glu Asn Ala Arg Met Phe Tyr Ala Phe Ile Gly Cys Ile Ile Ser Ala

35 40 45

Ile Thr Phe Pro Phe Ser Met Arg Ile Ile Gln Lys Met Val Ile Arg

50 55 60

Phe Thr Gly Lys Glu Phe Trp Gln Lys Asp Phe Phe Thr Asn Pro Val

65 70 75 80

Gly Gly Ser Leu Thr Ala Ile Phe Glu Leu Phe Cys Phe Val Ile Ser

85 90 95

Val Pro Val Val Ala Ile Tyr Leu Ile Phe Ile Leu Cys Lys Ala Leu

100 105 110

Ser Gly Lys

115

<210> 467

<211> 348

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 467

atgaaactgg atatatctgt aaagtattta ctgaaaagcc tgataccaat cctcattatt 60

cttacagttt tttatctggg atggaaagat aaccaggaaa atgcaagaat gttttatgcg 120

ttcatcggat gcattatcag tgccattact tttccttttt caatgaggat aatacagaaa 180

atggtaataa ggtttacagg gaaagaattc tggcaaaaag acttctttac aaatccagtt 240

ggcggaagct taactgcaat atttgaatta ttctgtttcg ttatatcagt tcctgtggtt 300

gccatttact taatttttat actctgcaaa gccctttcag gaaaatga 348

<210> 468

<211> 131

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 468

Met His Asn Thr Leu Leu Glu Lys Ile Ile Ala Tyr Leu Ser Leu Pro

1 5 10 15

Gly Phe His Ser Leu Asn Asn Pro Pro Leu Ser Glu Ala Phe Asn Leu

20 25 30

Tyr Val His Thr Ala Pro Leu Ala Ala Thr Ser Leu Phe Ile Phe Thr

35 40 45

His Lys Glu Leu Glu Leu Lys Pro Lys Ser Ser Pro Leu Arg Ala Leu

50 55 60

Lys Ile Leu Thr Pro Phe Thr Ile Leu Tyr Ile Ser Met Ile Tyr Cys

65 70 75 80

Phe Leu Leu Thr Asp Thr Glu Leu Thr Leu Ser Ser Lys Thr Phe Val

85 90 95

Leu Ile Val Lys Lys Arg Ser Val Phe Val Phe Phe Leu Tyr Asn Thr

100 105 110

Ile Tyr Trp Asp Ile Tyr Ile His Ile Phe Val Leu Leu Val Pro Tyr

115 120 125

Arg Asn Ile

130

<210> 469

<211> 396

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 469

atgcacaata cactcctcga aaaaatcatc gcatacctat ccctaccagg atttcattca 60

ttaaacaacc cgcccctaag cgaagcattc aatctctatg ttcatacagc ccctttagct 120

gcaaccagct tattcatatt cacacacaaa gaattagagt taaaaccaaa gtcgtcacct 180

ctgcgggcac taaagatatt aactcctttc actattcttt atatatccat gatatactgt 240

ttcttgctaa ctgacacaga actaaccttg tcatcaaaaa catttgtatt aatagtcaaa 300

aaacgatctg tttttgtctt ttttctatat aacactatat attgggatat atatattcac 360

atatttgtac ttttggttcc ttataggaac atataa 396

<210> 470

<211> 85

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 470

Met Glu Leu Lys Asn Ser Ile Ser Asp Tyr Thr Glu Thr Glu Phe Lys

1 5 10 15

Lys Ile Ile Glu Asp Ile Ile Asn Cys Glu Gly Asp Glu Lys Lys Gln

20 25 30

Asp Asp Asn Leu Glu His Phe Ile Ser Val Thr Glu His Pro Ser Gly

35 40 45

Ser Asp Leu Ile Tyr Tyr Pro Glu Gly Asn Asn Asp Gly Ser Pro Glu

50 55 60

Ala Val Ile Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly Lys Ser

65 70 75 80

Gly Phe Lys Gln Gly

85

<210> 471

<211> 258

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 471

atggaactga aaaacagcat tagtgattac actgaaactg aattcaaaaa aattattgaa 60

gacatcatca attgtgaagg tgatgaaaaa aaacaggatg ataacctcga gcattttata 120

agtgttactg agcatcctag tggttctgat ctgatttatt acccagaagg taataatgat 180

ggtagccctg aagctgttat taaagagatt aaagaatggc gagctgctaa cggtaagtca 240

ggatttaaac agggctga 258

<210> 472

<211> 98

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 472

Met Lys Lys Lys Gln Ile Glu Phe Glu Asn Glu Leu Arg Ser Met Leu

1 5 10 15

Ala Thr Ala Leu Glu Lys Asp Ile Ser Gln Glu Glu Arg Asn Ala Leu

20 25 30

Asn Ile Ala Glu Lys Ala Leu Asp Asn Ser Glu Tyr Leu Pro Lys Ile

35 40 45

Ile Leu Asn Leu Arg Lys Ala Leu Thr Pro Leu Ala Ile Asn Arg Thr

50 55 60

Leu Asn His Asp Leu Ser Glu Leu Tyr Lys Phe Ile Thr Ser Ser Lys

65 70 75 80

Ala Ser Asn Lys Asn Leu Gly Gly Gly Leu Ile Met Ser Trp Gly Arg

85 90 95

Leu Phe

<210> 473

<211> 297

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 473

atgaaaaaaa aacaaataga atttgaaaac gagctaagaa gtatgttggc taccgccctt 60

gaaaaagaca ttagtcaaga ggaaagaaat gctctgaata ttgcagaaaa ggcgcttgac 120

aattctgaat atttaccaaa aattatttta aacctcagaa aagccctaac tccattagct 180

ataaatcgaa cacttaacca tgatttatct gaactgtata aattcattac aagttccaaa 240

gcatcaaaca aaaatttagg tggtggttta attatgtcgt ggggacgact attctaa 297

<210> 474

<211> 98

<212> PRT

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 474

Met Lys Lys Lys Gln Ile Glu Phe Glu Asn Glu Leu Arg Ser Met Leu

1 5 10 15

Ala Thr Ala Leu Glu Lys Asp Ile Ser Gln Glu Glu Arg Asn Ala Leu

20 25 30

Asn Ile Ala Glu Lys Ala Leu Asp Asn Ser Glu Tyr Leu Pro Lys Ile

35 40 45

Ile Leu Asn Leu Arg Lys Ala Leu Thr Pro Leu Ala Ile Asn Arg Thr

50 55 60

Leu Asn His Asp Leu Ser Glu Leu Tyr Lys Phe Ile Thr Ser Ser Lys

65 70 75 80

Ala Ser Asn Lys Asn Leu Gly Gly Gly Leu Ile Met Ser Trp Gly Arg

85 90 95

Leu Phe

<210> 475

<211> 297

<212> DNA

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 475

atgaaaaaaa aacaaataga atttgaaaac gagctaagaa gtatgttggc taccgccctt 60

gaaaaagaca ttagtcaaga ggaaagaaat gctctgaata ttgcagaaaa ggcgcttgac 120

aattctgaat atttaccaaa aattatttta aacctcagaa aagccctaac tccattagct 180

ataaatcgaa cacttaacca tgatttatct gaactgtata aattcattac aagttccaaa 240

gcatcaaaca aaaatttagg tggtggttta attatgtcgt ggggacgact attctaa 297

<210> 476

<211> 87

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 476

Met Asn Lys Met Ala Met Ile Asp Leu Ala Lys Leu Phe Leu Ala Ser

1 5 10 15

Lys Ile Thr Ala Ile Glu Phe Ser Glu Arg Ile Cys Val Glu Arg Arg

20 25 30

Arg Leu Tyr Gly Val Lys Asp Leu Ser Pro Asn Ile Leu Asn Cys Gly

35 40 45

Glu Glu Leu Phe Met Ala Ala Glu Arg Phe Glu Pro Asp Ala Asp Arg

50 55 60

Ala Asn Tyr Glu Ile Asp Asp Asn Gly Leu Lys Val Glu Val Arg Ser

65 70 75 80

Ile Leu Glu Lys Phe Lys Leu

85

<210> 477

<211> 249

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 477

atgatcgatt tggcgaaatt atttttagct tcgaaaatta cagtgattga gttttcagag 60

cgaatttgtg ttgaacggag aagattgtat ggtgttaagg atttgtctcc gaatatatta 120

aattgtgggg aagagttgtc tatggctgct gagcgatttg agcctgatgc agatagggct 180

aattatgaaa ttgatgataa tggacttaag gtcgaggtcc gatctatctt ggaaaaactt 240

aaatcataa 249

<210> 478

<211> 83

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 478

Met Lys Leu Ser Pro Lys Ala Ala Ile Glu Val Cys Asn Glu Ala Ala

1 5 10 15

Lys Lys Gly Leu Trp Ile Leu Gly Ile Asp Gly Gly His Trp Leu Asn

20 25 30

Pro Gly Phe Arg Ile Asp Ser Ser Ala Ser Trp Thr Tyr Asp Met Pro

35 40 45

Glu Glu Tyr Lys Ser Lys Ile Pro Glu Asn Asn Arg Leu Ala Ile Glu

50 55 60

Asn Ile Lys Asp Asp Ile Glu Asn Gly Tyr Thr Ala Phe Ile Ile Thr

65 70 75 80

Leu Lys Met

<210> 479

<211> 243

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 479

atgaagttat caccaaaagc tgcaatagaa gtttgtaatg aagcagcgaa aaaaggctta 60

tggattttgg gcattgatgg tgggcattgg ctgaatcctg gattcaggat agatagttca 120

gcatcatgga catatgatat gccggagaat acaaatcaaa aatccctgaa aataatagat 180

tggctattga aaatattaaa gatgatattg agaatggata cactgctttc attatcacgt 240

taa 243

<210> 480

<211> 85

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 480

Met Gly Leu Lys Leu His Ile Asn Trp Phe Asp Lys Arg Thr Glu Glu

1 5 10 15

Phe Lys Gly Gly Glu Tyr Ser Lys Asp Phe Gly Asp Asp Gly Ser Val

20 25 30

Ile Glu Arg Leu Gly Met Pro Phe Lys Asp Asn Ile Asn Asn Gly Trp

35 40 45

Phe Asp Val Ile Ala Glu Trp Val Pro Leu Leu Gln Pro Tyr Phe Asn

50 55 60

His Gln Ile Asp Ile Ser Asp Asn Glu Tyr Phe Val Ser Phe Asp Tyr

65 70 75 80

Arg Asp Gly Asp Trp

85

<210> 481

<211> 258

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 481

atggggctta aattacatat taattggttt gataagacga ccgaggaatt taaaggtggt 60

gagtattcaa aagattttgg agatgatggc tcggtcattg aacgtcttgg aatgccttta 120

aaagataata tcaataatgg ttggtttgat gttatagctg aatgggtacc tttgctacaa 180

ccatacttta atcatcaaat tgatatttcc gataatgagt attttgtttc gtttgattat 240

cgtgatggtg attggtga 258

<210> 482

<211> 85

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 482

Met Glu Leu Lys Lys Ser Ile Gly Asp Tyr Thr Glu Thr Glu Phe Lys

1 5 10 15

Lys Ile Ile Glu Asn Ile Ile Asn Cys Glu Gly Asp Glu Lys Lys Gln

20 25 30

Asp Asp Asn Leu Glu His Phe Ile Ser Val Thr Glu His Pro Ser Gly

35 40 45

Ser Asp Leu Ile Tyr Tyr Pro Glu Gly Asn Asn Asp Gly Ser Pro Glu

50 55 60

Ala Val Ile Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly Lys Ser

65 70 75 80

Gly Phe Lys Gln Gly

85

<210> 483

<211> 258

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 483

gtggagctaa agaaaagtat tggtgattac actgaaaccg aattcaaaaa aattattgaa 60

aacatcatca attgtgaagg tgatgaaaaa aaacaggatg ataacctcga gcattttata 120

agtgttactg agcatcctag tggttctgat ctgatttatt acccagaagg taataatgat 180

ggtagccctg aagctgttat taaagagatt aaagaatggc gagctgctaa cggtaagtca 240

ggatttaaac agggctga 258

<210> 484

<211> 86

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 484

Met Glu Leu Lys His Ser Ile Ser Asp Tyr Thr Glu Ala Glu Phe Leu

1 5 10 15

Gln Leu Val Thr Thr Ile Cys Asn Ala Asp Thr Ser Ser Glu Glu Glu

20 25 30

Leu Val Lys Leu Val Thr His Phe Glu Glu Met Thr Glu His Pro Ser

35 40 45

Gly Ser Asp Leu Ile Tyr Tyr Pro Lys Glu Gly Asp Asp Asp Ser Pro

50 55 60

Ser Gly Ile Val Asn Thr Val Lys Gln Trp Arg Ala Ala Asn Gly Lys

65 70 75 80

Ser Gly Phe Lys Gln Gly

85

<210> 485

<211> 261

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 485

atggaactga agcatagcat tagtgattat acagaagctg aatttttaca acttgtaaca 60

acaatttgta atgcgaacac ttccagtgaa gaagaactgg ttaaattggt tacacacttt 120

gaggaaatga ctgagcaccc tagtggtagt gatttaatat attacccaaa agaaggtgat 180

gatgactcac cttcaggtat tgtaaacaca gtaaaacaat ggcgagccgc taacggtaag 240

tcaggattta aacagggcta a 261

<210> 486

<211> 141

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 486

Met Leu Thr Leu Tyr Gly Tyr Ile Arg Asn Val Phe Leu Tyr Arg Met

1 5 10 15

Asn Asp Arg Ser Cys Gly Asp Phe Met Lys Val Ile Ser Met Lys Phe

20 25 30

Ile Phe Ile Leu Thr Ile Ile Ala Leu Ala Ala Val Phe Phe Trp Ser

35 40 45

Glu Asp Lys Gly Pro Ala Cys Tyr Gln Val Ser Asp Glu Gln Ala Arg

50 55 60

Thr Phe Val Lys Asn Asp Tyr Leu Gln Arg Met Lys Arg Trp Asp Asn

65 70 75 80

Asp Val Gln Leu Leu Gly Thr Glu Ile Pro Lys Ile Thr Trp Glu Lys

85 90 95

Ile Glu Arg Ser Leu Thr Asp Val Glu Asp Glu Lys Thr Leu Leu Val

100 105 110

Pro Phe Lys Ala Glu Gly Pro Asp Gly Lys Arg Met Tyr Tyr Gly Met

115 120 125

Tyr His Cys Glu Glu Gly Tyr Val Glu Tyr Ala Asn Asp

130 135 140

<210> 487

<211> 354

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 487

atgaaagtaa ttagcatgaa atttattttt attttaacga ttattgctct tgctgctgtt 60

tttttctggt ctgaagataa aggtccggca tgctatcagg tcagcgatga acaggccaga 120

acgtttgtaa aaaatgatta cctgcaaaga atgaaacgct gggacaacga tgtacaactt 180

cttggtacag aaatcccgaa aattacatgg gaaaagattg agagaagttt aacagatgtt 240

gaagatgaaa aaacacttct tgtcccattt aaagctgaag gcccggacgg taagagaatg 300

tattatggca tgtaccattg tgaggaggga tatgttgaat atgcgaatga ctaa 354

<210> 488

<211> 175

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 488

Met Thr Ser Asn Lys Asp Lys Asn Lys Lys Ala Asn Glu Ile Leu Tyr

1 5 10 15

Ala Phe Ser Ile Ile Gly Ile Ile Pro Leu Met Ala Ile Leu Ile Leu

20 25 30

Arg Ile Asn Asp Pro Tyr Ser Gln Val Leu Tyr Tyr Leu Tyr Asn Lys

35 40 45

Val Ala Phe Leu Pro Ser Ile Thr Ser Leu His Asp Pro Val Met Thr

50 55 60

Thr Leu Met Ser Asn Tyr Asn Lys Thr Ala Pro Val Met Gly Ile Leu

65 70 75 80

Val Phe Leu Cys Thr Tyr Lys Thr Arg Glu Ile Ile Lys Pro Val Thr

85 90 95

Arg Lys Leu Val Val Gln Ser Cys Phe Trp Gly Pro Val Phe Tyr Ala

100 105 110

Ile Leu Ile Tyr Ile Thr Leu Phe Tyr Asn Leu Glu Leu Thr Thr Ala

115 120 125

Gly Gly Phe Phe Lys Leu Leu Ser His Asn Val Ile Thr Leu Phe Ile

130 135 140

Leu Tyr Cys Ser Ile Tyr Phe Thr Val Leu Thr Met Thr Tyr Ala Ile

145 150 155 160

Leu Leu Met Pro Leu Leu Val Ile Lys Tyr Phe Lys Gly Arg Gln

165 170 175

<210> 489

<211> 528

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 489

atgaccagca ataaagataa gaacaagaaa gcaaacgaaa tattatatgc attttccata 60

atcgggatta ttccattaat ggctatatta atacttcgaa taaatgatcc atattctcaa 120

gtgctgtact acttatataa taaggtggca tttctccctt ctattacatc attgcatgat 180

cccgtcatga caacacttat gtcaaactac aacaagacag cgccagttat gggtattctc 240

gtttttcttt gcacatataa gacaagagaa atcataaagc cagtaacaag aaaacttgtt 300

gtgcaatcct gtttctgggg gcccgttttt tatgccattc tgatttatat cacactgttc 360

tataatctgg aactaacaac agcaggtggt ttttttaaat tattatctca taatgtcatc 420

actctgttta ttttatattg ctccatttac tttactgttt taaccatgac atatgcgatt 480

ttactgatgc cattacttgt cattaaatat tttaaaggga ggcagtaa 528

<210> 490

<211> 78

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 490

Met Asp Arg Lys Arg Thr Lys Leu Glu Leu Leu Phe Ala Phe Ile Ile

1 5 10 15

Asn Ala Thr Ala Ile Tyr Ile Ala Leu Ala Ile Tyr Asp Cys Val Phe

20 25 30

Arg Gly Lys Asp Phe Leu Ser Met His Thr Phe Cys Phe Ser Ala Leu

35 40 45

Met Ser Ala Ile Cys Tyr Phe Val Gly Asp Asn Tyr Tyr Ser Ile Ser

50 55 60

Asp Lys Ile Lys Arg Arg Ser Tyr Glu Asn Ser Asp Ser Lys

65 70 75

<210> 491

<211> 237

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 491

atggatagaa aaagaacaaa attagagttg ttatttgcat ttataataaa tgccaccgca 60

atatatattg cattagctat atatgattgt gtttttagag gaaaggactt tttatccatg 120

catacatttt gcttctctgc attaatgtct gcaatatgtt actttgttgg tgataattat 180

tattcaatat ccgataagat aaaaaggaga tcatatgaga actctgactc taaatga 237

<210> 492

<211> 113

<212> PRT

<213> 宋内志贺菌(Shigella sonnei)

<400> 492

Met Ser Leu Arg Tyr Tyr Ile Lys Asn Ile Leu Phe Gly Leu Tyr Cys

1 5 10 15

Ala Leu Ile Tyr Ile Tyr Leu Ile Thr Lys Asn Asn Glu Gly Tyr Tyr

20 25 30

Phe Leu Ala Ser Asp Lys Met Leu Tyr Ala Ile Val Ile Ser Thr Ile

35 40 45

Leu Cys Pro Tyr Ser Lys Tyr Ala Ile Glu His Ile Phe Phe Lys Phe

50 55 60

Ile Lys Lys Asp Phe Phe Arg Lys Arg Lys Asn Leu Asn Lys Cys Pro

65 70 75 80

Arg Gly Lys Ile Lys Pro Tyr Leu Cys Val Tyr Asn Leu Leu Cys Leu

85 90 95

Val Leu Ala Ile Pro Phe Gly Leu Leu Gly Leu Val Tyr Ile Asn Lys

100 105 110

Glu

<210> 493

<211> 342

<212> DNA

<213> 宋内志贺菌(Shigella sonnei)

<400> 493

atgagtttaa gatactacat aaaaaatatt ttgtttggcc tatactgcgc acttatatat 60

atatacctta taacaaaaaa caacgaaggg tattatttcc tagcgtcaga taagatgcta 120

tacgcaatag tgataagcac tattctatgc ccatattcaa aatatgctat tgaacacata 180

ttttttaagt tcataaagaa agattttttc agaaaaagaa aaaacctaaa taaatgcccc 240

cgtggcaaaa ttaaaccgta tttatgcgta tacaatctac tttgtttggt cctagcaatc 300

ccatttggat tgctaggact tgtttatatc aataaagaat aa 342

<210> 494

<211> 113

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 494

Met Ser Leu Arg Tyr Tyr Ile Lys Asn Ile Leu Phe Gly Leu Tyr Cys

1 5 10 15

Thr Leu Ile Tyr Ile Tyr Leu Ile Thr Lys Asn Ser Glu Glu Tyr Tyr

20 25 30

Phe Leu Val Thr Asp Lys Met Leu Tyr Ala Ile Val Ile Ser Thr Ile

35 40 45

Leu Cys Pro Tyr Ser Lys Tyr Ala Ile Glu His Ile Ala Phe Asn Phe

50 55 60

Ile Lys Lys His Phe Phe Glu Arg Arg Lys Asn Leu Asn Asn Ala Pro

65 70 75 80

Val Ala Lys Leu Asn Leu Phe Met Leu Tyr Asn Leu Leu Cys Leu Val

85 90 95

Leu Ala Ile Pro Phe Gly Leu Leu Gly Leu Phe Ile Ser Ile Lys Asn

100 105 110

Asn

<210> 495

<211> 342

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 495

atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac acttatatat 60

atatacctta taacaaaaaa cagcgaagag tattatttcc ttgtgacaga taagatgcta 120

tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat tgaacacata 180

gcttttaact tcataaagaa acattttttc gaaagaagaa aaaacctaaa taacgccccc 240

gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct agcaatccca 300

tttggattgc taggactttt tatatcaata aagaataatt aa 342

<210> 496

<211> 113

<212> PRT

<213> 冷生明串珠菌(Leuconostoc gelidum)

<400> 496

Met Arg Lys Asn Asn Ile Leu Leu Asp Asp Ala Lys Ile Tyr Thr Asn

1 5 10 15

Lys Leu Tyr Leu Leu Leu Ile Asp Arg Lys Asp Asp Ala Gly Tyr Gly

20 25 30

Asp Ile Cys Asp Val Leu Phe Gln Val Ser Lys Lys Leu Asp Ser Thr

35 40 45

Lys Asn Val Glu Ala Leu Ile Asn Arg Leu Val Asn Tyr Ile Arg Ile

50 55 60

Thr Ala Ser Thr Asn Arg Ile Lys Phe Ser Lys Asp Glu Glu Ala Val

65 70 75 80

Ile Ile Glu Leu Gly Val Ile Gly Gln Lys Ala Gly Leu Asn Gly Gln

85 90 95

Tyr Met Ala Asp Phe Ser Asp Lys Ser Gln Phe Tyr Ser Ile Phe Glu

100 105 110

Arg

<210> 497

<211> 342

<212> DNA

<213> 冷生明串珠菌(Leuconostoc gelidum)

<400> 497

ttgagaaaaa ataacatttt attggacgat gctaaaatat acacgaacaa actctatttg 60

ctattaatcg atagaaaaga tgacgctggg tatggagata tttgtgatgt tttgtttcag 120

gtatccaaaa aattagatag cacaaaaaat gtagaagcat tgattaaccg attggtcaat 180

tatatacgaa ttaccgcttc aacaaacaga attaagtttt caaaagatga agaggctgta 240

attatagaac ttggtgtaat tggtcagaag gctggattaa acggccaata catggctgat 300

ttttctgaca aatctcagtt ttatagtatc tttgaaagat aa 342

<210> 498

<211> 91

<212> PRT

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 498

Met Lys Lys Lys Val Asp Thr Glu Lys Gln Ile Thr Ser Trp Ala Ser

1 5 10 15

Asp Leu Ala Ser Lys Asn Glu Thr Lys Val Gln Glu Lys Leu Ile Leu

20 25 30

Ser Ser Tyr Ile Gln Asp Ile Glu Asn His Val Tyr Phe Pro Lys Ala

35 40 45

Met Ile Ser Leu Glu Lys Lys Leu Arg Asp Gln Asn Asn Ile Cys Ala

50 55 60

Leu Ser Lys Glu Val Asn Gln Phe Tyr Phe Lys Val Val Glu Val Asn

65 70 75 80

Gln Arg Lys Ser Trp Met Val Gly Leu Ile Val

85 90

<210> 499

<211> 276

<212> DNA

<213> 乳酸乳球菌乳脂亚种(Lactococcus lactis subsp. cremoris)

<400> 499

atgaaaaaaa aagttgatac agaaaaacaa attacttctt gggcatctga cttagcttcc 60

aaaaatgaaa caaaggttca agaaaaatta atactgtctt cttatattca ggacatcgaa 120

aaccatgttt actttccaaa agcaatgatt tctttagaaa aaaaattacg agaccaaaat 180

aatatttgcg ctttatcaaa agaagtcaat cagttttatt ttaaagttgt tgaagtaaat 240

caaagaaaat cctggatggt aggtttgata gtttaa 276

<210> 500

<211> 112

<212> PRT

<213> 嗜酸小球菌(Pediococcus acidilactici)

<400> 500

Met Asn Lys Thr Lys Ser Glu His Ile Lys Gln Gln Ala Leu Asp Leu

1 5 10 15

Phe Thr Arg Leu Gln Phe Leu Leu Gln Lys His Asp Thr Ile Glu Pro

20 25 30

Tyr Gln Tyr Val Leu Asp Ile Leu Glu Thr Gly Ile Ser Lys Thr Lys

35 40 45

His Asn Gln Gln Thr Pro Glu Arg Gln Ala Arg Val Val Tyr Asn Lys

50 55 60

Ile Ala Ser Gln Ala Leu Val Asp Lys Leu His Phe Thr Ala Glu Glu

65 70 75 80

Asn Lys Val Leu Ala Ala Ile Asn Glu Leu Ala His Ser Gln Lys Gly

85 90 95

Trp Gly Glu Phe Asn Met Leu Asp Thr Thr Asn Thr Trp Pro Ser Gln

100 105 110

<210> 501

<211> 339

<212> DNA

<213> 嗜酸小球菌(Pediococcus acidilactici)

<400> 501

atgaataaga ctaagtcgga acatattaaa caacaagctt tggacttatt tactaggcta 60

cagtttttac tacagaagca cgatactatc gaaccttacc agtacgtttt agatattctg 120

gagactggta tcagtaaaac taaacataac cagcaaacgc ctgaacgaca agctcgtgta 180

gtctacaaca agattgccag ccaagcgtta gtagataagt tacattttac tgccgaagaa 240

aacaaagttc tagcagccat caatgaattg gcgcattctc aaaaagggtg gggcgagttt 300

aacatgctag atactaccaa tacgtggcct agccaatag 339

<210> 502

<211> 88

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 502

Met Ile Lys Asp Glu Lys Ile Asn Lys Ile Tyr Ala Leu Val Lys Ser

1 5 10 15

Ala Leu Asp Asn Thr Asp Val Lys Asn Asp Lys Lys Leu Ser Leu Leu

20 25 30

Leu Met Arg Ile Gln Glu Thr Ser Ile Asn Gly Glu Leu Phe Tyr Asp

35 40 45

Tyr Lys Lys Glu Leu Gln Pro Ala Ile Ser Met Tyr Ser Ile Gln His

50 55 60

Asn Phe Arg Val Pro Asp Asp Leu Val Lys Leu Leu Ala Leu Val Gln

65 70 75 80

Thr Pro Lys Ala Trp Ser Gly Phe

85

<210> 503

<211> 267

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 503

atgataaaag atgaaaaaat aaataaaatc tatgctttag ttaagagcgc acttgataat 60

acggatgtta agaatgataa aaaactttct ttacttctta tgagaataca agaaacatca 120

attaatggag aactatttta cgattataaa aaagaattac agccagctat tagtatgtac 180

tctattcaac ataactttcg ggttcctgac gatctagtaa aactgttagc attagttcaa 240

acacctaaag cttggtcagg gttttaa 267

<210> 504

<211> 111

<212> PRT

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 504

Met Asp Ile Lys Ser Gln Thr Leu Tyr Leu Asn Leu Ser Glu Ala Tyr

1 5 10 15

Lys Asp Pro Glu Val Lys Ala Asn Glu Phe Leu Ser Lys Leu Val Val

20 25 30

Gln Cys Ala Gly Lys Leu Thr Ala Ser Asn Ser Glu Asn Ser Tyr Ile

35 40 45

Glu Val Ile Ser Leu Leu Ser Arg Gly Ile Ser Ser Tyr Tyr Leu Ser

50 55 60

His Lys Arg Ile Ile Pro Ser Ser Met Leu Thr Ile Tyr Thr Gln Ile

65 70 75 80

Gln Lys Asp Ile Lys Asn Gly Asn Ile Asp Thr Glu Lys Leu Arg Lys

85 90 95

Tyr Glu Ile Ala Lys Gly Leu Met Ser Val Pro Tyr Ile Tyr Phe

100 105 110

<210> 505

<211> 336

<212> DNA

<213> 麦芽芳香卡诺杆菌(Carnobacterium maltaromaticum)

<400> 505

atggatataa agtctcaaac attatatttg aatctaagcg aggcatataa agaccctgaa 60

gtaaaagcta atgaattctt atcaaaatta gttgtacaat gtgctgggaa attaacagct 120

tcaaacagtg agaacagtta tattgaagta atatcattgc tatctagggg tatttctagt 180

tattatttat cccataaacg tataattcct tcaagtatgt taactatata tactcaaata 240

caaaaggata taaaaaacgg gaatattgac accgaaaaat taaggaaata tgagatagca 300

aaaggattaa tgtccgttcc ttatatatat ttctaa 336

<210> 506

<211> 245

<212> PRT

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 506

Met Arg Arg Tyr Leu Ile Leu Ile Val Ala Leu Ile Gly Ile Thr Gly

1 5 10 15

Leu Ser Gly Cys Tyr Gln Thr Ser His Lys Lys Val Arg Phe Asp Glu

20 25 30

Gly Ser Tyr Thr Asn Phe Ile Tyr Asp Asn Lys Ser Tyr Phe Val Thr

35 40 45

Asp Lys Glu Ile Pro Gln Glu Asn Val Asn Asn Ser Lys Val Lys Phe

50 55 60

Tyr Lys Leu Leu Ile Val Asp Met Lys Ser Glu Lys Leu Leu Ser Ser

65 70 75 80

Ser Asn Lys Asn Ser Val Thr Leu Val Leu Asn Asn Ile Tyr Glu Ala

85 90 95

Ser Asp Lys Ser Leu Cys Met Gly Ile Asn Asp Arg Tyr Tyr Lys Ile

100 105 110

Leu Pro Glu Ser Asp Lys Gly Ala Val Lys Ala Leu Arg Leu Gln Asn

115 120 125

Phe Asp Val Thr Ser Asp Ile Ser Asp Asp Asn Phe Val Ile Asp Lys

130 135 140

Asn Asp Ser Arg Lys Ile Asp Tyr Met Gly Asn Ile Tyr Ser Ile Ser

145 150 155 160

Asp Thr Thr Val Ser Asp Glu Glu Leu Gly Glu Tyr Gln Asp Val Leu

165 170 175

Ala Glu Val Arg Val Phe Asp Ser Val Ser Gly Lys Ser Ile Pro Arg

180 185 190

Ser Glu Trp Gly Arg Ile Asp Lys Asp Gly Ser Asn Ser Lys Gln Ser

195 200 205

Arg Thr Glu Trp Asp Tyr Gly Glu Ile His Ser Ile Arg Gly Lys Ser

210 215 220

Leu Thr Glu Ala Phe Ala Val Glu Ile Asn Asp Asp Phe Lys Leu Ala

225 230 235 240

Thr Lys Val Gly Asn

245

<210> 507

<211> 738

<212> DNA

<213> 乳酸乳球菌乳酸亚种(Lactococcus lactis subsp. lactis)

<400> 507

atgagaagat atttaatact tattgtggcc ttaataggga taacaggttt atcagggtgt 60

tatcaaacaa gtcataaaaa ggtgaggttt gacgaaggaa gttatactaa ttttatttat 120

gataataaat cgtatttcgt aactgataag gagattcctc aggagaacgt taacaattcc 180

aaagtaaaat tttataagct gttgattgtt gacatgaaaa gtgagaaact tttatcaagt 240

agcaacaaaa atagtgtgac tttggtctta aataatattt atgaggcttc tgacaagtcg 300

ctatgtatgg gtattaacga cagatactat aagatacttc cagaaagtga taagggggcg 360

gtcaaagctt tgagattaca aaactttgat gtgacaagcg atatttctga tgataatttt 420

gttattgata aaaatgattc acgaaaaatt gactatatgg gaaatattta cagtatatcg 480

gacaccaccg tatctgatga agaattggga gaatatcagg atgttttagc tgaagtacgt 540

gtgtttgatt cagttagtgg caaaagtatc ccgaggtctg aatgggggag aattgataag 600

gatggttcaa attccaaaca gagtaggacg gaatgggatt atggcgaaat ccattctatt 660

agaggaaaat ctcttactga agcatttgcc gttgagataa atgatgattt taagcttgca 720

acgaaggtag gaaactag 738

<210> 508

<211> 261

<212> PRT

<213> 根瘤菌三叶草生物型(Rhizobium leguminosarum bv. trifolii)

<400> 508

Met Asn Asp Glu Ile Cys Leu Thr Gly Gly Gly Arg Thr Thr Val Thr

1 5 10 15

Arg Arg Gly Gly Val Val Tyr Arg Glu Gly Gly Pro Trp Ser Ser Thr

20 25 30

Val Ile Ser Leu Leu Arg His Leu Glu Ala Ser Gly Phe Ala Glu Ala

35 40 45

Pro Ser Val Val Gly Thr Gly Phe Asp Glu Arg Gly Arg Glu Thr Leu

50 55 60

Ser Phe Ile Glu Gly Glu Phe Val His Pro Gly Pro Trp Ser Glu Glu

65 70 75 80

Ala Phe Pro Gln Phe Gly Met Met Leu Arg Arg Leu His Asp Ala Thr

85 90 95

Ala Ser Phe Lys Pro Pro Glu Asn Ser Met Trp Arg Asp Trp Phe Gly

100 105 110

Arg Asn Leu Gly Glu Gly Gln His Val Ile Gly His Cys Asp Thr Gly

115 120 125

Pro Trp Asn Ile Val Cys Arg Ser Gly Leu Pro Val Gly Leu Ile Asp

130 135 140

Trp Glu Val Ala Gly Pro Val Arg Ala Asp Ile Glu Leu Ala Gln Ala

145 150 155 160

Cys Trp Leu Asn Ala Gln Leu Tyr Asp Asp Asp Ile Ala Glu Arg Val

165 170 175

Gly Leu Gly Ser Val Thr Met Arg Ala His Gln Val Arg Leu Leu Leu

180 185 190

Asp Gly Tyr Gly Leu Ser Arg Lys Gln Arg Gly Gly Phe Val Asp Lys

195 200 205

Leu Ile Thr Phe Ala Val His Asp Ala Ala Glu Gln Ala Lys Glu Ala

210 215 220

Ala Val Thr Pro Glu Ser Asn Asp Ala Glu Pro Leu Trp Ala Ile Ala

225 230 235 240

Trp Arg Thr Arg Ser Ala Ser Trp Met Leu His His Arg Gln Thr Leu

245 250 255

Glu Ala Ala Leu Ala

260

<210> 509

<211> 786

<212> DNA

<213> 根瘤菌三叶草生物型(Rhizobium leguminosarum bv. trifolii)

<400> 509

atgaatgatg agatttgcct gacaggtggc ggacgaacga ctgtcacgcg gcgcggcgga 60

gtcgtgtatc gcgaaggcgg cccgtggtca tcaaccgtca tttcgctcct gcggcatctg 120

gaagcctctg gcttcgctga agctccttcc gttgtcggca ccggtttcga tgagcgcggc 180

cgggagacat tatcgtttat cgagggtgag tttgttcacc caggcccttg gtcggaggag 240

gcttttccgc aatttggaat gatgttgcgg cgactgcacg atgccaccgc ctcgttcaaa 300

cctcccgaaa actcgatgtg gcgcgattgg ttcgggcgta acctcggtga gggtcaacac 360

gtaataggac actgcgacac aggcccatgg aacattgttt gccggtcagg attgcctgtc 420

gggttgatag attgggaggt ggctgggcct gtcagggcgg atatcgaatt ggcccaggct 480

tgttggctga atgcccagct ctacgatgac gacattgcgg agagggtcgg attaggctct 540

gtgaccatga gagcgcatca agttcgcctg ctgcttgacg gctatggtct gtctcggaag 600

caacgcggcg gcttcgtcga caagctaatc acgttcgcag ttcacgatgc ggccgagcag 660

gcgaaagagg cggctgtcac gccagagtcg aacgatgcgg aaccgctatg ggcaattgcc 720

tggcgcacta gaagtgcctc ctggatgctc catcatcggc aaacactgga agcagcgctg 780

gcatag 786

<210> 510

<211> 436

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 510

Met Asn Asn Ile Ile Pro Ile Met Ser Leu Leu Phe Lys Gln Leu Tyr

1 5 10 15

Ser Arg Gln Gly Lys Lys Asp Ala Ile Arg Ile Ala Ala Gly Leu Val

20 25 30

Ile Leu Ala Val Phe Glu Ile Gly Leu Ile Arg Gln Ala Gly Ile Asp

35 40 45

Glu Ser Val Leu Arg Lys Thr Tyr Ile Ile Leu Ala Leu Leu Leu Met

50 55 60

Asn Thr Tyr Met Val Phe Leu Ser Val Thr Ser Gln Trp Lys Glu Ser

65 70 75 80

Tyr Met Lys Leu Ser Cys Leu Leu Pro Ile Ser Ser Arg Ser Phe Trp

85 90 95

Leu Ala Gln Ser Val Val Leu Phe Val Asp Thr Cys Leu Arg Arg Thr

100 105 110

Leu Phe Phe Phe Ile Leu Pro Leu Phe Leu Phe Gly Asn Gly Thr Leu

115 120 125

Ser Gly Ala Gln Thr Leu Phe Trp Leu Gly Arg Phe Ser Phe Phe Thr

130 135 140

Val Tyr Ser Ile Ile Phe Gly Val Val Leu Ser Asn His Phe Val Lys

145 150 155 160

Lys Lys Asn Leu Met Phe Leu Leu His Ala Ala Ile Phe Ala Cys Val

165 170 175

Cys Ile Ser Ala Ala Leu Met Pro Ala Ala Thr Ile Pro Leu Cys Ala

180 185 190

Val His Ile Leu Trp Ala Val Val Ile Asp Phe Pro Val Phe Leu Gln

195 200 205

Ala Pro Pro Gln Gln Gly Lys Met His Ser Phe Met Arg Arg Ser Glu

210 215 220

Phe Ser Phe Tyr Lys Arg Glu Trp Asn Arg Phe Ile Ser Ser Lys Ala

225 230 235 240

Met Leu Leu Asn Tyr Ala Val Met Ala Val Phe Ser Gly Phe Phe Ser

245 250 255

Phe Gln Met Met Asn Thr Gly Ile Phe Asn Gln Gln Val Ile Tyr Ile

260 265 270

Val Ile Ser Ala Leu Leu Leu Ile Cys Ser Pro Ile Ala Leu Leu Tyr

275 280 285

Ser Ile Glu Lys Asn Asp Arg Met Leu Leu Ile Thr Leu Pro Ile Lys

290 295 300

Arg Lys Thr Met Phe Trp Ala Lys Tyr Arg Phe Tyr Ser Gly Leu Leu

305 310 315 320

Ala Gly Gly Phe Leu Leu Val Val Met Ile Val Gly Phe Ile Ser Gly

325 330 335

Arg Ser Ile Ser Val Leu Thr Phe Leu Gln Cys Ile Glu Leu Leu Leu

340 345 350

Ala Gly Ala Tyr Ile Arg Leu Thr Ala Asp Glu Lys Arg Pro Ser Phe

355 360 365

Ser Trp Gln Thr Glu Gln Gln Leu Trp Ser Gly Phe Ser Lys Tyr Arg

370 375 380

Ser Tyr Leu Phe Cys Leu Pro Leu Phe Leu Ala Ile Leu Ala Gly Thr

385 390 395 400

Ala Val Ser Leu Ala Val Ile Pro Ile Ala Gly Leu Val Ile Val Tyr

405 410 415

Tyr Leu Gln Lys Gln Asp Gly Gly Phe Phe Asp Thr Ser Lys Arg Glu

420 425 430

Arg Leu Gly Ser

435

<210> 511

<211> 1000

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 511

atgaataaca taatccctat catgtctttg ctgttcaaac agctttacag ccggcaaggg 60

aaaaaggacg ccatccgcat tgccgcaggc cttgtcattc tggccgtgtt tgaaatcggg 120

ctgatccgcc aggccggcat tgatgaatcg gtgttgcgca aaacgtatat catactcgcg 180

cttcttttga tgaacacata tatggtgttt ctttccgtga catcacaatg gaaggaatct 240

tatatgaagc tgagctgcct gctgccgatt tcttcacgga gcttttggct cgcccagagt 300

gtcgttttgt ttgtcgatac ctgtttgaga agaactttat tcttttttat tttaccgctg 360

ttcttatttg gaaacggaac gctgtcaggg gcgcaaacat tgttttggct cggcaggttt 420

tcgtttttta ccgtttactc cattattttc ggagttgtgc taagcaacca cttcgtcaaa 480

aagaagaact tgatgtttct gctgcatgcg gcgatattcg cctgtgtatg tatcagcgcc 540

gctttgatgc cggccgccac gattccgctt tgcgcggttc atatcctgtg ggcggtggtc 600

attgactttc ctgtctttct gcaggcgcct ccgcagcagg gcaagatgca ttcatttatg 660

cggcgatctg aattttcgtt ttacaaaaga gaatggaacc gatttatctc ttctaaagcg 720

atgctgttaa attacgcggt aatggcggta ttcagcggct tcttttcgtt ccagatgatg 780

aacaccggca tcttcaatca gcaagtgatt tatatcgtga tttccgcgct tttgctcatc 840

tgctcgccga tcgccctttt gtattcgatt gaaaaaaatg accggatgct gctcatcacg 900

cttccgatca agcgaaaaac gatgttttgg gcgaaatatc gcttttattc aggcctattg 960

gcaggcggat ttctccttgt cgtgatgatt gtgggtttca 1000

<210> 512

<211> 239

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 512

Met Ser Ile Leu Asp Ile His Asp Val Ser Val Trp Tyr Glu Arg Asp

1 5 10 15

Asn Val Ile Leu Glu Gln Val Asp Leu His Leu Glu Lys Gly Ala Val

20 25 30

Tyr Gly Leu Leu Gly Val Asn Gly Ala Gly Lys Thr Thr Leu Ile Asn

35 40 45

Thr Leu Thr Gly Val Asn Arg Asn Phe Ser Gly Arg Phe Thr Leu Cys

50 55 60

Gly Ile Glu Ala Glu Ala Gly Met Pro Gln Lys Thr Ser Asp Gln Leu

65 70 75 80

Lys Thr His Arg Tyr Phe Ala Ala Asp Tyr Pro Leu Leu Phe Thr Glu

85 90 95

Ile Thr Ala Lys Asp Tyr Val Ser Phe Val His Ser Leu Tyr Gln Lys

100 105 110

Asp Phe Ser Glu Gln Gln Phe Ala Ser Leu Ala Glu Ala Phe His Phe

115 120 125

Ser Lys Tyr Ile Asn Arg Arg Ile Ser Glu Leu Ser Leu Gly Asn Arg

130 135 140

Gln Lys Val Val Leu Met Thr Gly Leu Leu Leu Arg Ala Pro Leu Phe

145 150 155 160

Ile Leu Asp Glu Pro Leu Val Gly Leu Asp Val Glu Ser Ile Glu Val

165 170 175

Phe Tyr Gln Lys Met Arg Glu Tyr Cys Glu Ala Gly Gly Thr Ile Leu

180 185 190

Phe Ser Ser His Leu Leu Asp Val Val Gln Arg Phe Cys Asp Tyr Ala

195 200 205

Ala Ile Leu His Asn Lys Gln Ile Gln Lys Val Ile Pro Ile Gly Glu

210 215 220

Glu Thr Asp Leu Arg Arg Glu Phe Phe Glu Val Ile Gly His Glu

225 230 235

<210> 513

<211> 716

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 513

gcattttgga tatacacgat gtatccgttt ggtatgaacg ggacaacgtc atcttagagc 60

acgtggactt acacttagaa aaaggcgccg tttacggatt gcttggggta aacggtgccg 120

gcaaaacaac actgatcaat acgctgacag gagtgaaccg caattacagc gggggcttta 180

cgctgtgcgg cattgaagct gaggccggca tgccgcagaa aacatcagat caactgaaga 240

ttcaccgtta cttcgccgct gattatccgc tgctgtttac agaaattacg gcgaaggact 300

atgtgtcttt cgtccattcg ctttatcaaa aggatttttc agagcgacag tttgccagtt 360

tggctgaggc ctttcatttt tcaaaataca tcaacaggag aatctcggag ctgtccttgg 420

ggaacaggca aaaggttgtg ttgatgacag gattattgct gcgggctccc ctgtttattt 480

tggatgagcc gctcgtcggt ttggatgtgg aatcaataga ggtcttttat cagaaaatgc 540

gggagtactg tgaggaaggc ggaaccattt tgttttcttc ccatctgctc gatgtcgtgc 600

agagattttg tgattttgcg gccattctgc acaacaaaca gatccaaaag gtcattccga 660

ttggggagga gaccgatctg cggcgggaat tttttgaggt tatcggccat gaataa 716

<210> 514

<211> 53

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 514

Met Ser Pro Ala Gln Arg Arg Ile Leu Leu Tyr Ile Leu Ser Phe Ile

1 5 10 15

Phe Val Ile Gly Ala Val Val Tyr Phe Val Lys Ser Asp Tyr Leu Phe

20 25 30

Thr Leu Ile Phe Ile Ala Ile Ala Ile Leu Phe Gly Met Arg Ala Arg

35 40 45

Lys Ala Asp Ser Arg

50

<210> 515

<211> 162

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 515

ttgtcaccag cacaaagaag aattttactg tatatccttt catttatctt tgtcatcggc 60

gcagtcgtct attttgtcaa aagcgattat ctgtttacgc tgattttcat tgccattgcc 120

attctgttcg ggatgcgcgc gcggaaggct gactcgcgat ga 162

<210> 516

<211> 87

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 516

Met Glu Leu Lys Asn Ser Ile Ser Asp Tyr Thr Glu Ala Glu Phe Val

1 5 10 15

Gln Leu Leu Lys Glu Ile Glu Lys Glu Asn Val Ala Ala Thr Asp Asp

20 25 30

Val Leu Asp Val Leu Leu Glu His Phe Val Lys Ile Thr Glu His Pro

35 40 45

Asp Gly Thr Asp Leu Ile Tyr Tyr Pro Ser Asp Asn Arg Asp Asp Ser

50 55 60

Pro Glu Gly Ile Val Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly

65 70 75 80

Lys Pro Gly Phe Lys Gln Gly

85

<210> 517

<211> 264

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 517

atggaactga aaaatagtat tagtgattac acagaggctg agtttgttca acttcttaag 60

gaaattgaaa aagagaatgt tgctgcaact gatgatgtgt tagatgtgtt actcgaacac 120

tttgtaaaaa ttactgagca tccagatgga acggatctga tttattatcc tagtgataat 180

agagacgata gccccgaagg gattgtcaag gaaattaaag aatggcgagc tgctaacggt 240

aagccaggat ttaaacaggg ctga 264

<210> 518

<211> 87

<212> PRT

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 518

Met Lys Ser Lys Ile Ser Glu Tyr Thr Glu Lys Glu Phe Leu Glu Phe

1 5 10 15

Val Glu Asp Ile Tyr Thr Asn Asn Lys Lys Lys Phe Pro Thr Glu Glu

20 25 30

Ser His Ile Gln Ala Val Leu Glu Phe Lys Lys Leu Thr Glu His Pro

35 40 45

Ser Gly Ser Asp Leu Leu Tyr Tyr Pro Asn Glu Asn Arg Glu Asp Ser

50 55 60

Pro Ala Gly Val Val Lys Glu Val Lys Glu Trp Arg Ala Ser Lys Gly

65 70 75 80

Leu Pro Gly Phe Lys Ala Gly

85

<210> 519

<211> 264

<212> DNA

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 519

atgaagtcca agatttccga atatacggaa aaagagtttc ttgagtttgt tgaagacata 60

tacacaaaca ataagaaaaa gttccctacc gaggagtctc atattcaagc cgtgcttgaa 120

tttaaaaaac taacggaaca cccaagcggc tcagaccttc tttactaccc caacgaaaat 180

agagaagata gcccagctgg agttgtaaag gaagttaaag aatggcgtgc ttccaagggg 240

cttcctggct ttaaggccgg ttag 264

<210> 520

<211> 87

<212> PRT

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 520

Met Lys Ser Lys Ile Ser Glu Tyr Thr Glu Lys Glu Phe Leu Glu Phe

1 5 10 15

Val Lys Asp Ile Tyr Thr Asn Asn Lys Lys Lys Phe Pro Thr Glu Glu

20 25 30

Ser His Ile Gln Ala Val Leu Glu Phe Lys Lys Leu Thr Glu His Pro

35 40 45

Ser Gly Ser Asp Leu Leu Tyr Tyr Pro Asn Glu Asn Arg Glu Asp Ser

50 55 60

Pro Ala Gly Val Val Lys Glu Val Lys Glu Trp Arg Ala Ser Lys Gly

65 70 75 80

Leu Pro Gly Phe Lys Ala Gly

85

<210> 521

<211> 264

<212> DNA

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 521

atgaagtcca agatttccga atatacggaa aaagagtttc ttgagtttgt taaagacata 60

tacacaaaca ataagaaaaa gttccctacc gaggagtctc atattcaagc cgtgcttgaa 120

tttaaaaaac taacggaaca cccaagcggc tcagaccttc tttactaccc caacgaaaat 180

agagaagata gcccagctgg agttgtaaag gaagttaaag aatggcgtgc ttccaagggg 240

cttcctggct ttaaggccgg ttag 264

<210> 522

<211> 95

<212> PRT

<213> 海氏肠球菌(Enterococcus hirae)

<400> 522

Met Asp Phe Thr Lys Glu Glu Lys Leu Leu Asn Ala Ile Ser Lys Val

1 5 10 15

Tyr Asn Glu Ala Thr Ile Asp Asp Tyr Pro Asp Leu Lys Glu Lys Leu

20 25 30

Phe Leu Tyr Ser Lys Glu Ile Ser Glu Gly Lys Ser Val Gly Glu Val

35 40 45

Ser Met Lys Leu Ser Ser Phe Leu Gly Arg Tyr Ile Leu Lys His Lys

50 55 60

Phe Gly Leu Pro Lys Ser Leu Ile Glu Leu Gln Glu Ile Val Ser Lys

65 70 75 80

Glu Ser Gln Val Tyr Arg Gly Trp Ala Ser Ile Gly Ile Trp Ser

85 90 95

<210> 523

<211> 288

<212> DNA

<213> 海氏肠球菌(Enterococcus hirae)

<400> 523

atggatttta ctaaagaaga aaaactttta aatgcaatta gtaaagtata caatgaagca 60

actatagatg actatcctga cttaaaagaa aagctctttc tttattctaa agaaatcagt 120

gagggaaaaa gtgttggtga agttagtatg aaattaagta gttttcttgg aagatatatt 180

ttaaaacata aatttggatt acctaaatct ttaatagaat tacaagaaat tgttagtaag 240

gaatctcaag tatatagagg atgggcttct attggtattt ggagttaa 288

<210> 524

<211> 113

<212> PRT

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 524

Met Lys Lys Lys Tyr Arg Tyr Leu Glu Asp Ser Lys Asn Tyr Thr Ser

1 5 10 15

Thr Leu Tyr Ser Leu Leu Val Asp Asn Val Asp Lys Pro Gly Tyr Ser

20 25 30

Asp Ile Cys Asp Val Leu Leu Gln Val Ser Lys Lys Leu Asp Asn Thr

35 40 45

Gln Ser Val Glu Ala Leu Ile Asn Arg Leu Val Asn Tyr Ile Arg Ile

50 55 60

Thr Ala Ser Thr Tyr Lys Ile Ile Phe Ser Lys Lys Glu Glu Glu Leu

65 70 75 80

Ile Ile Lys Leu Gly Val Ile Gly Gln Lys Ala Gly Leu Asn Gly Gln

85 90 95

Tyr Met Ala Asp Phe Ser Asp Lys Ser Gln Phe Tyr Ser Val Phe Asp

100 105 110

Gln

<210> 525

<211> 342

<212> DNA

<213> 肠膜明串珠菌(Leuconostoc mesenteroides)

<400> 525

ttgaaaaaaa agtatcggta tttagaagat agcaaaaatt acactagtac actctattct 60

ctgttagttg ataatgttga caaacctgga tactcagata tttgcgatgt tttgcttcaa 120

gtttctaaga agttggataa tactcaaagt gttgaagcgc taattaatcg attggttaat 180

tatattcgta ttactgcttc aacatacaaa attatttttt caaaaaaaga agaggaattg 240

attataaaac ttggtgttat tggacaaaaa gctggactta atggtcagta tatggctgat 300

ttttcagaca agtctcagtt ttacagcgtt ttcgatcagt aa 342

<210> 526

<211> 93

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 526

Met Ser Phe Leu Asn Phe Ala Phe Ser Pro Val Phe Phe Ser Ile Met

1 5 10 15

Ala Cys Tyr Phe Ile Val Trp Arg Asn Lys Arg Asn Glu Phe Val Cys

20 25 30

Asn Arg Leu Leu Ser Ile Ile Ile Ile Ser Phe Leu Ile Cys Phe Ile

35 40 45

Tyr Pro Trp Leu Asn Tyr Lys Ile Glu Val Lys Tyr Tyr Ile Phe Glu

50 55 60

Gln Phe Tyr Leu Phe Cys Phe Leu Ser Ser Leu Val Ala Val Val Ile

65 70 75 80

Asn Leu Ile Val Tyr Phe Ile Leu Tyr Arg Arg Cys Ile

85 90

<210> 527

<211> 282

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 527

atgagttttc ttaattttgc attttctcct gtattcttct ccattatggc gtgttatttc 60

attgtatgga gaaataaacg aaacgaattt gtctgcaata gattgctatc aattataata 120

atatcttttt tgatatgctt catatatcca tggctaaatt acaaaatcga agttaaatat 180

tatatatttg aacagtttta tcttttttgt tttttatcgt cactcgtggc tgttgtaata 240

aacctaattg tatactttat attatacagg agatgtatat ga 282

<210> 528

<211> 96

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 528

Met His Leu Lys Tyr Tyr Leu His Asn Leu Pro Glu Ser Leu Ile Pro

1 5 10 15

Trp Ile Leu Ile Leu Ile Phe Asn Asp Asn Asp Asn Thr Pro Leu Leu

20 25 30

Phe Ile Phe Ile Ser Ser Ile His Val Leu Leu Tyr Pro Tyr Ser Lys

35 40 45

Leu Thr Ile Ser Arg Tyr Ile Lys Glu Asn Thr Lys Leu Lys Lys Glu

50 55 60

Pro Trp Tyr Leu Cys Lys Leu Ser Ala Leu Phe Tyr Leu Leu Met Ala

65 70 75 80

Ile Pro Val Gly Leu Pro Ser Phe Ile Tyr Tyr Thr Leu Lys Arg Asn

85 90 95

<210> 529

<211> 291

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 529

atgcatttaa aatactacct acataattta cctgaatcac ttataccatg gattcttatt 60

ttaatattta acgacaatga taacactcct ttgttattta tatttatatc atcaatacat 120

gtattgctat atccatactc taaattaacc atatctagat atatcaaaga aaatacaaag 180

ttaaaaaaag aaccctggta cttatgcaag ttatctgcat tgttttattt attaatggca 240

atcccagtag gattgccaag tttcatatat tacactctaa agagaaatta a 291

<210> 530

<211> 344

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 530

Met Met Ile Gln Ser His Pro Leu Leu Ala Ala Pro Leu Ala Val Gly

1 5 10 15

Asp Thr Ile Gly Phe Phe Ser Ser Ser Ala Pro Ala Thr Val Thr Ala

20 25 30

Lys Asn Arg Phe Phe Arg Gly Val Glu Phe Leu Gln Arg Lys Gly Phe

35 40 45

Lys Leu Val Ser Gly Lys Leu Thr Gly Lys Thr Asp Phe Tyr Arg Ser

50 55 60

Gly Thr Ile Lys Glu Arg Ala Gln Glu Phe Asn Glu Leu Val Tyr Asn

65 70 75 80

Pro Asp Ile Thr Cys Ile Met Ser Thr Ile Gly Gly Asp Asn Ser Asn

85 90 95

Ser Leu Leu Pro Phe Leu Asp Tyr Asp Ala Ile Ile Ala Asn Pro Lys

100 105 110

Ile Ile Ile Gly Tyr Ser Asp Thr Thr Ala Leu Leu Ala Gly Ile Tyr

115 120 125

Ala Lys Thr Gly Leu Ile Thr Phe Tyr Gly Pro Ala Leu Ile Pro Ser

130 135 140

Phe Gly Glu His Pro Pro Leu Val Asp Ile Thr Tyr Glu Ser Phe Ile

145 150 155 160

Lys Ile Leu Thr Arg Lys Gln Ser Gly Ile Tyr Thr Tyr Thr Leu Pro

165 170 175

Glu Lys Trp Ser Asp Glu Ser Ile Asn Trp Asn Glu Asn Lys Ile Leu

180 185 190

Arg Pro Lys Lys Leu Tyr Lys Asn Asn Cys Ala Phe Tyr Gly Ser Gly

195 200 205

Lys Val Glu Gly Arg Val Ile Gly Gly Asn Leu Asn Thr Leu Thr Gly

210 215 220

Ile Trp Gly Ser Glu Trp Met Pro Glu Ile Leu Asn Gly Asp Ile Leu

225 230 235 240

Phe Ile Glu Asp Ser Arg Lys Ser Ile Ala Thr Ile Glu Arg Leu Phe

245 250 255

Ser Met Leu Lys Leu Asn Arg Val Phe Asp Lys Val Ser Ala Ile Ile

260 265 270

Leu Gly Lys His Glu Leu Phe Asp Cys Ala Gly Ser Lys Arg Arg Pro

275 280 285

Tyr Glu Val Leu Thr Glu Val Leu Asp Gly Lys Gln Ile Pro Val Leu

290 295 300

Asp Gly Phe Asp Cys Ser His Thr His Pro Met Leu Thr Leu Pro Leu

305 310 315 320

Gly Val Lys Leu Ala Ile Asp Phe Asp Asn Lys Asn Ile Ser Ile Thr

325 330 335

Glu Gln Tyr Leu Ser Thr Glu Lys

340

<210> 531

<211> 1000

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 531

atgatgatac aatctcatcc actactggcc gctcccctgg cagtaggaga tacaattggt 60

ttcttttcat catctgctcc ggcaacagtt actgcaaaaa atcgtttttt tcggggagtt 120

gagtttcttc agagaaaggg atttaagctg gtatcaggga agcttaccgg taaaacagat 180

ttttatcgtt caggtactat taaagaaaga gctcaagaat ttaatgagtt agtctacaat 240

cctgatatta cctgtataat gtcaacgatc ggtggagata acagtaattc actactaccg 300

tttctggact atgatgctat cattgcaaac cccaaaatta tcataggtta ctcagataca 360

actgctttat tagcaggaat atatgcaaaa acagggttaa taacattcta tggaccagct 420

cttattcctt cgtttggtga acatccacct cttgtggata taacatatga atcatttatt 480

aaaatactaa caagaaaaca atcaggaata tatacctaca cattacctga aaagtggagt 540

gatgagagca taaactggaa tgaaaacaag atattaaggc ctaagaagct atataaaaac 600

aactgtgcct tttatggttc cggaaaagtt gaggggcgtg taattggagg aaatctaaat 660

actttgacag gtatatgggg gagtgaatgg atgcctgaaa ttcttaatgg agatatattg 720

tttattgagg acagtcggaa aagcattgca acaattgaac gattattctc tatgctaaag 780

cttaatcgcg tgtttgataa agttagtgca ataatactcg ggaaacatga gctttttgat 840

tgtgcaggaa gtaaacgcag accatatgaa gtattaacag aggtattaga tgggaaacag 900

attcctgtac tggatggatt tgattgttca catacacatc caatgctaac tcttccactt 960

ggtgtaaaat tagctattga ctttgacaac aaaaatatat 1000

<210> 532

<211> 90

<212> PRT

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 532

Met Lys Ala Asp Tyr Lys Lys Ile Asn Ser Ile Leu Thr Tyr Thr Ser

1 5 10 15

Thr Ala Leu Lys Asn Pro Lys Ile Ile Lys Asp Lys Asp Leu Val Val

20 25 30

Leu Leu Thr Ile Ile Gln Glu Glu Ala Lys Gln Asn Arg Ile Phe Tyr

35 40 45

Asp Tyr Lys Arg Lys Phe Arg Pro Ala Val Thr Arg Phe Thr Ile Asp

50 55 60

Asn Asn Phe Glu Ile Pro Asp Cys Leu Val Lys Leu Leu Ser Ala Val

65 70 75 80

Glu Thr Pro Lys Ala Trp Ser Gly Phe Ser

85 90

<210> 533

<211> 268

<212> DNA

<213> 清酒乳杆菌(Lactobacillus sakei)

<400> 533

ggcagattat aaaaaaataa attcaatact aacttacaca tctactgctt taaaaaaccc 60

taaaattata aaagataaag atttagtagt ccttctaact attattcaag aagaagccaa 120

acaaaataga atcttttatg attataaaag aaaatttcgt ccagcggtta ctcgctttac 180

aattgataat aattttgaga ttcctgattg tttggttaaa ctactgtcag ctgttgaaac 240

acctaaggcg tggtctggat ttagttag 268

<210> 534

<211> 83

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 534

Met Lys Leu Ser Pro Lys Ala Ala Ile Glu Val Cys Asn Glu Ala Ala

1 5 10 15

Lys Lys Gly Leu Trp Ile Leu Gly Ile Asp Gly Gly His Trp Leu Asn

20 25 30

Pro Gly Phe Arg Ile Asp Ser Ser Ala Ser Trp Thr Tyr Asp Met Pro

35 40 45

Glu Glu Tyr Lys Ser Lys Thr Pro Glu Asn Asn Arg Leu Ala Ile Glu

50 55 60

Asn Ile Lys Asp Asp Ile Glu Asn Gly Tyr Thr Ala Phe Ile Ile Thr

65 70 75 80

Leu Lys Met

<210> 535

<211> 251

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 535

tgaagttatc accaaaagct gcaatagaag tttgtaatga agcagcgaaa aaaggcttat 60

ggattttggg cattgatggt gggcattggc tgaatcctgg attcaggata gatagttcag 120

catcatggac atatgatatg ccggaggaat acaaatcaaa aacccctgaa aataatagat 180

tggctattga aaatattaaa gatgatattg agaatggata cactgctttc attatcacgt 240

taaagatgta a 251

<210> 536

<211> 436

<212> PRT

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 536

Met Asn Asn Ile Phe Pro Ile Met Ser Leu Leu Phe Lys Gln Leu Tyr

1 5 10 15

Ser Arg Gln Gly Lys Lys Asp Ala Ile Arg Ile Ala Ala Gly Leu Val

20 25 30

Ile Leu Ala Val Phe Glu Ile Gly Leu Ile Arg Gln Ala Gly Ile Asp

35 40 45

Glu Ser Val Leu Gly Lys Thr Tyr Ile Ile Leu Ala Leu Leu Leu Met

50 55 60

Asn Thr Tyr Met Val Phe Leu Ser Val Thr Ser Gln Trp Lys Glu Ser

65 70 75 80

Tyr Met Lys Leu Ser Cys Leu Leu Pro Ile Ser Ser Arg Ser Phe Trp

85 90 95

Leu Ala Gln Ser Val Val Leu Phe Val Asp Thr Cys Leu Arg Arg Thr

100 105 110

Leu Phe Phe Phe Ile Leu Pro Leu Phe Leu Phe Gly Asn Gly Thr Leu

115 120 125

Ser Gly Ala Gln Thr Leu Phe Trp Leu Gly Arg Phe Ser Phe Phe Thr

130 135 140

Val Tyr Ser Ile Leu Phe Gly Val Met Leu Ser Asn His Phe Val Lys

145 150 155 160

Lys Lys Asn Ser Met Phe Leu Leu His Ala Ala Val Phe Ala Phe Val

165 170 175

Cys Leu Ser Ala Ala Phe Met Pro Ala Val Thr Ile Pro Leu Cys Ala

180 185 190

Val His Met Leu Trp Ala Val Ile Ile Asp Phe Pro Val Phe Leu Gln

195 200 205

Ala Pro Pro His Gln Ser Lys Met His Phe Phe Met Arg Arg Ser Glu

210 215 220

Phe Ser Phe Tyr Lys Arg Glu Trp Asn Arg Phe Ile Ser Ser Lys Ala

225 230 235 240

Met Leu Leu Asn Tyr Val Val Met Ala Ala Phe Ser Gly Phe Phe Ser

245 250 255

Phe Gln Met Met Asn Thr Gly Ile Phe Asn Gln Gln Val Ile Tyr Ile

260 265 270

Val Ile Ser Ala Leu Leu Leu Ile Cys Ser Pro Ile Ala Leu Leu Tyr

275 280 285

Ser Ile Glu Lys Asn Asp Arg Met Leu Leu Ile Thr Leu Pro Ile Lys

290 295 300

Arg Arg Thr Met Phe Trp Ala Lys Tyr Arg Phe Tyr Ser Gly Leu Leu

305 310 315 320

Ala Gly Gly Phe Leu Leu Val Ala Ile Ile Val Gly Phe Ile Ser Gly

325 330 335

Arg Pro Ile Ser Ala Leu Thr Phe Val Gln Cys Met Glu Leu Leu Leu

340 345 350

Ala Gly Ala Phe Ile Arg Leu Thr Ala Asp Glu Lys Arg Pro Ser Phe

355 360 365

Gly Trp Gln Thr Glu Gln Gln Leu Trp Ser Gly Phe Ser Lys Tyr Arg

370 375 380

Ser Tyr Leu Phe Cys Leu Pro Leu Phe Leu Ala Thr Leu Ala Gly Thr

385 390 395 400

Ala Val Ser Leu Ala Val Ile Pro Ile Ala Ala Leu Ile Ile Val Tyr

405 410 415

Tyr Leu Gln Lys Gln Asp Gly Gly Phe Phe Asp Thr Ser Lys Arg Glu

420 425 430

Arg Ile Gly Ser

435

<210> 537

<211> 1000

<212> DNA

<213> 枯草芽孢杆菌(Bacillus subtilis)

<400> 537

ttggggagga gaccgatctg cggcgggaat tttttgaggt tatcggccat gaataacata 60

ttccccatca tgtcgttgct gttcaaacag ctgtacagcc ggcaagggaa aaaggacgct 120

atccgcattg ctgcagggct tgtgattctc gccgtgtttg aaatcgggct gatccgacaa 180

gccggcattg acgaatcggt gttgggaaaa acgtatatca tattggcgct tctcttaatg 240

aacacgtata tggtgtttct ttccgtgaca tcacaatgga aggaatctta tatgaagctg 300

agctgtctgc tgccgatttc atcacggagc ttttggctcg cccagagtgt cgttctgttt 360

gtcgatacct gtttgagaag aacgttattc ttttttattt taccgctgtt cttatttgga 420

aacggaacgc tgtcaggggc gcaaacattg ttttggcttg gcagattttc gttttttacc 480

gtttactcga ttctattcgg agttatgcta agcaaccatt tcgtcaaaaa gaagaactcg 540

atgtttctgc tgcatgcggc ggtattcgcc tttgtatgcc tcagtgccgc ttttatgccg 600

gccgtcacga tcccgctatg cgcggttcac atgctatggg cggtgatcat tgactttccg 660

gtctttctgc aggcgcctcc gcatcagagc aagatgcatt tttttatgcg gcgatctgaa 720

ttttcgtttt acaaaagaga atggaaccga tttatttctt ctaaagcgat gctgttaaat 780

tacgtggtga tggcggcgtt cagcggattc ttttcgttcc agatgatgaa cactggcatc 840

ttcaatcagc aagtgattta tattgtgatt tccgctctat tgctgatttg ctcgccgatc 900

gcccttttgt actctattga aaaaaacgat cgcatgctgc tcatcacgct tccaattaaa 960

agaagaacga tgttttgggc gaaatatcgc ttttattcag 1000

<210> 538

<211> 580

<212> PRT

<213> 大肠杆菌(Escherichia coli)

<400> 538

Met Glu Arg Lys Gln Lys Asn Ser Leu Phe Asn Tyr Ile Tyr Ser Leu

1 5 10 15

Met Asp Val Arg Gly Lys Phe Leu Phe Phe Ser Met Leu Phe Ile Thr

20 25 30

Ser Leu Ser Ser Ile Ile Ile Ser Ile Ser Pro Leu Ile Leu Ala Lys

35 40 45

Ile Thr Asp Leu Leu Ser Gly Ser Leu Ser Asn Phe Ser Tyr Glu Tyr

50 55 60

Leu Val Leu Leu Ala Cys Leu Tyr Met Phe Cys Val Ile Ser Asn Lys

65 70 75 80

Ala Ser Val Phe Leu Phe Met Ile Leu Gln Ser Ser Leu Arg Ile Asn

85 90 95

Met Gln Lys Lys Met Ser Leu Lys Tyr Leu Arg Glu Leu Tyr Asn Glu

100 105 110

Asn Ile Thr Asn Leu Ser Lys Asn Asn Ala Gly Tyr Thr Thr Gln Ser

115 120 125

Leu Asn Gln Ala Ser Asn Asp Ile Tyr Ile Leu Val Arg Asn Val Ser

130 135 140

Gln Asn Ile Leu Ser Pro Val Ile Gln Leu Ile Ser Thr Ile Val Val

145 150 155 160

Val Leu Ser Thr Lys Asp Trp Phe Ser Ala Gly Val Phe Phe Leu Tyr

165 170 175

Ile Leu Val Phe Val Ile Phe Asn Thr Arg Leu Thr Gly Ser Leu Ala

180 185 190

Ser Leu Arg Lys His Ser Met Asp Ile Thr Leu Asn Ser Tyr Ser Leu

195 200 205

Leu Ser Asp Thr Val Asp Asn Met Ile Ala Ala Lys Lys Asn Asn Ala

210 215 220

Leu Arg Leu Ile Ser Glu Arg Tyr Glu Asp Ala Leu Thr Gln Glu Asn

225 230 235 240

Asn Ala Gln Lys Lys Tyr Trp Leu Leu Ser Ser Lys Val Leu Leu Leu

245 250 255

Asn Ser Leu Leu Ala Val Ile Leu Phe Gly Ser Val Phe Ile Tyr Asn

260 265 270

Ile Leu Gly Val Leu Asn Gly Val Val Ser Ile Gly His Phe Ile Met

275 280 285

Ile Thr Ser Tyr Ile Ile Leu Leu Ser Thr Pro Val Glu Asn Ile Gly

290 295 300

Ala Leu Leu Ser Glu Ile Arg Gln Ser Met Ser Ser Leu Ala Gly Phe

305 310 315 320

Ile Gln Arg His Ala Glu Asn Lys Ala Thr Ser Pro Ser Ile Pro Phe

325 330 335

Leu Asn Met Glu Arg Lys Leu Asn Leu Ser Ile Arg Glu Leu Ser Phe

340 345 350

Ser Tyr Ser Asp Asp Lys Lys Ile Leu Asn Ser Val Ser Leu Asp Leu

355 360 365

Phe Thr Gly Lys Met Tyr Ser Leu Thr Gly Pro Ser Gly Ser Gly Lys

370 375 380

Ser Thr Leu Val Lys Ile Ile Ser Gly Tyr Tyr Lys Asn Tyr Phe Gly

385 390 395 400

Asp Ile Tyr Leu Asn Asp Ile Ser Leu Arg Asn Ile Ser Asp Glu Asp

405 410 415

Leu Asn Asp Ala Ile Tyr Tyr Leu Thr Gln Asp Asp Tyr Ile Phe Met

420 425 430

Asp Thr Leu Arg Phe Asn Leu Arg Leu Ala Asn Tyr Asp Ala Ser Glu

435 440 445

Asn Glu Ile Phe Lys Val Leu Lys Leu Ala Asn Leu Ser Val Val Asn

450 455 460

Asn Glu Pro Val Ser Leu Asp Thr His Leu Ile Asn Arg Gly Asn Asn

465 470 475 480

Tyr Ser Gly Gly Gln Lys Gln Arg Ile Ser Leu Ala Arg Leu Phe Leu

485 490 495

Arg Lys Pro Ala Ile Ile Ile Ile Asp Glu Ala Thr Ser Ala Leu Asp

500 505 510

Tyr Ile Asn Glu Ser Glu Ile Leu Ser Ser Ile Arg Thr His Phe Pro

515 520 525

Asp Ala Leu Ile Ile Asn Ile Ser His Arg Ile Asn Leu Leu Glu Cys

530 535 540

Ser Asp Cys Val Tyr Val Leu Asn Glu Gly Asn Ile Val Ala Ser Gly

545 550 555 560

His Phe Arg Asp Leu Met Val Ser Asn Glu Tyr Ile Ser Gly Leu Ala

565 570 575

Ser Val Thr Glu

580

<210> 539

<211> 1000

<212> DNA

<213> 大肠杆菌(Escherichia coli)

<400> 539

atggaaagaa aacagaaaaa ctcattattt aattatattt attcattaat ggatgtaaga 60

ggtaaatttt tattcttttc catgttattc attacatcat tatcatcgat aatcatatct 120

atttcaccat tgattcttgc aaagattaca gatttactgt ctggctcatt gtcaaatttt 180

agttatgaat atctggtttt acttgcctgt ttatacatgt tttgcgttat atctaataaa 240

gcaagtgttt ttttatttat gatactgcaa agtagtctac gtattaacat gcagaaaaaa 300

atgtcgctaa agtatttgag agaattgtat aacgaaaata taactaactt gagtaaaaat 360

aatgctggat atacaacgca aagtcttaac caggcttcaa atgacattta tattcttgtg 420

agaaatgttt cccagaatat cctgtcacct gttatacaac ttatttccac tattgttgtt 480

gttttatcta cgaaggactg gttttctgcc ggtgtgtttt ttctctatat tctggtattt 540

gtaattttta ataccagact gactggcagt ttagcgtctc tcagaaaaca cagcatggat 600

atcactctta actcttatag tctgttatct gatactgttg ataacatgat agcagctaaa 660

aagaataatg cattaagact tatttctgaa cgttatgaag atgctctcac tcaggaaaac 720

aatgctcaga aaaaatactg gttactcagt tctaaagttc ttttattgaa ctctttactt 780

gctgtaatat tatttggttc tgtattcata tataatattt taggtgtgct gaatggtgta 840

gttagtatcg gccacttcat tatgattaca tcatatatca ttcttctttc aacgccagtg 900

gaaaatatag gggcattgct aagtgagatc aggcagtcaa tgtctagcct ggcaggtttt 960

attcaacgtc atgccgagaa taaagccaca tctccttcaa 1000

<210> 540

<211> 95

<212> PRT

<213> 肺炎克雷伯菌(Klebsiella pneumoniae)

<400> 540

Met Thr Leu Leu Ser Phe Gly Phe Ser Pro Val Phe Phe Ser Val Met

1 5 10 15

Ala Phe Cys Ile Ile Ser Arg Ser Lys Phe Tyr Pro Gln Arg Thr Arg

20 25 30

Asn Lys Val Ile Val Leu Ile Leu Leu Thr Phe Phe Ile Cys Phe Leu

35 40 45

Tyr Pro Leu Thr Lys Val Tyr Leu Val Gly Ser Tyr Gly Ile Phe Asp

50 55 60

Lys Phe Tyr Leu Phe Cys Phe Ile Ser Thr Leu Ile Ala Ile Ala Ile

65 70 75 80

Asn Val Val Ile Leu Thr Ile Asn Gly Ala Lys Asn Glu Arg Asn

85 90 95

<210> 541

<211> 288

<212> DNA

<213> 肺炎克雷伯菌(Klebsiella pneumoniae)

<400> 541

atgacattac tttcatttgg attttctcct gttttctttt cagtcatggc gttctgtatc 60

atttcacgta gtaaattcta tccgcagaga acgcgaaaca aagttattgt tctgatttta 120

ctaacttttt ttatttgttt tttatatcca ttaacaaaag tgtatctggt gggaagttac 180

ggtatatttg acaaattcta cctcttttgc tttatttcta cgttaattgc aatagcaatt 240

aacgtagtga tacttacaat aaatggagct aagaatgaga gaaattag 288

<210> 542

<211> 13

<212> RNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Kozak序列

<400> 542

gccgccrcca ugg 13

<210> 543

<211> 6

<212> RNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Shine-Delgarno序列

<400> 543

ggaggu 6

<210> 544

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 引导启动子

<400> 544

gaaaaccttg tcaatgaaga gcgatctatg 30

<210> 545

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> FecA启动子

<400> 545

ttctcgttcg actcatagct gaacacaaca 30

<210> 546

<211> 16

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Cu敏感性启动子

<400> 546

atgacaaaat tgtcat 16

<210> 547

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Fe启动子

<400> 547

accaatgctg ggaacggcca gggcacctaa 30

<210> 548

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Fe和UV启动子

<400> 548

ctgaaagcgc ataccgctat ggagggggtt 30

<210> 549

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> PrFe (PI+PII rus操纵子)

<400> 549

tagatatgcc tgaaagcgca taccgctatg 30

<210> 550

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Lux盒右侧启动子

<400> 550

tgttatagtc gaatacctct ggcggtgata 30

<210> 551

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> P(Las) TetO

<400> 551

ttttggtaca ctccctatca gtgatagaga 30

<210> 552

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> P(Las) CIO

<400> 552

ctttttggta cactacctct ggcggtgata 30

<210> 553

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> P(Rhl)

<400> 553

tacgcaagaa aatggtttgt tatagtcgaa 30

<210> 554

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 双启动子(LuxR/HSL, 阳性/ cI,

阴性)

<400> 554

cgtgcgtgtt gataacaccg tgcgtgttga 30

<210> 555

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 来自金黄色葡萄球菌的agr操纵子中的P2启动子

<400> 555

agattgtact aaatcgtata atgacagtga 30

<210> 556

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> plux-cI杂合启动子

<400> 556

gtgttgatgc ttttatcacc gccagtggta 30

<210> 557

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> plux-lac杂合启动子

<400> 557

agtgtgtgga attgtgagcg gataacaatt 30

<210> 558

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> CinR, CinL和葡萄糖受控的启动子

<400> 558

acatcttaaa agttttagta tcatattcgt 30

<210> 559

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> CI可阻遏的RhIR启动子

<400> 559

tacgcaagaa aatggtttgt tatagtcgaa 30

<210> 560

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 反向Lux启动子

<400> 560

tcttgcgtaa acctgtacga tcctacaggt 30

<210> 561

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> rhlI启动子

<400> 561

atcctccttt agtcttcccc ctcatgtgtg 30

<210> 562

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> lasI启动子

<400> 562

taaaattatg aaatttgcat aaattcttca 30

<210> 563

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> LuxR+3OC6HSL独立的R0065

<400> 563

gtgttgacta ttttacctct ggcggtgata 30

<210> 564

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> LasR/LasI诱导的& RHLR/RHLI可阻遏的启动子

<400> 564

gaaatctggc agtttttggt acacgaaagc 30

<210> 565

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pLux/cI杂合启动子

<400> 565

acaccgtgcg tgttgatata gtcgaataaa 30

<210> 566

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pLas启动子

<400> 566

aaaattatga aatttgtata aattcttcag 30

<210> 567

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pLas/cI杂合启动子

<400> 567

ggttcttttt ggtacctctg gcggtgataa 30

<210> 568

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pLas/Lux杂合启动子

<400> 568

tgtaggatcg tacaggtata aattcttcag 30

<210> 569

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pLux

<400> 569

caagaaaatg gtttgttata gtcgaataaa 30

<210> 570

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pLux/Las杂合启动子

<400> 570

ctatctcatt tgctagtata gtcgaataaa 30

<210> 571

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>杂合启动子：HSL-LuxR活化的，P22 C2阻遏的

<400> 571

tagtttataa tttaagtgtt ctttaatttc 30

<210> 572

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> PAI+LasR -> LuxI (AI)

<400> 572

caccttcggg tgggcctttc tgcgtttata 30

<210> 573

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> PAI+LasR -> LasI & AI+LuxR --| LasI

<400> 573

aataactctg atagtgctag tgtagatctc 30

<210> 574

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP

<400> 574

caccttcggg tgggcctttc tgcgtttata 30

<210> 575

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 复杂的QS -> LuxI & LasI回路

<400> 575

caccttcggg tgggcctttc tgcgtttata 30

<210> 576

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 位置3突变的启动子lux pR-3 (luxR & HSL调节的)

<400> 576

caagaaaatg gtttgttata gtcgaataaa 30

<210> 577

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 位置5突变的启动子lux pR-5 (luxR & HSL调节的)

<400> 577

caagaaaatg gtttgttata gtcgaataaa 30

<210> 578

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>位置3&5突变的启动子lux pR-3/5 (luxR &HSL调节的)

<400> 578

caagaaaatg gtttgttata gtcgaataaa 30

<210> 579

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子(HSL介导的luxR阻遏物)

<400> 579

ttgacacctg taggatcgta caggtataat 30

<210> 580

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子(luxR & HSL调节的-- lux pR)

<400> 580

caagaaaatg gtttgttata gtcgaataaa 30

<210> 581

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子(luxR & HSL调节的-- lux pL)

<400> 581

cacgcaaaac ttgcgacaaa caataggtaa 30

<210> 582

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子(RhlR & C4-HSL调节的)

<400> 582

gttagctttc gaattggcta aaaagtgttc 30

<210> 583

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子(cinR和HSL调节的)

<400> 583

ccattctgct ttccacgaac ttgaaaacgc 30

<210> 584

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子(LasR & PAI调节的)

<400> 584

ggccgcgggt tctttttggt acacgaaagc 30

<210> 585

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子，标准的(luxR和HSL调节的—lux pR)

<400> 585

aagaaaatgg tttgttgata ctcgaataaa 30

<210> 586

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> P(Bla)

<400> 586

gtttatacat aggcgagtac tctgttatgg 30

<210> 587

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> P(Cat)

<400> 587

agaggttcca actttcacca taatgaaaca 30

<210> 588

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> P(Kat)

<400> 588

taaacaacta acggacaatt ctacctaaca 30

<210> 589

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 用于构建引物家族成员的模板

<400> 589

acatcaagcc aaattaaaca ggattaacac 30

<210> 590

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 反向λ cI-调节的启动子

<400> 590

gaggtaaaat agtcaacacg cacggtgtta 30

<210> 591

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 关键启动子吸收3

<400> 591

caggccggaa taactcccta taatgcgcca 30

<210> 592

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 592

ggctagctca gtcctaggta cagtgctagc 30

<210> 593

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 593

agctagctca gtcctaggta ttatgctagc 30

<210> 594

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 594

agctagctca gtcctaggta ctgtgctagc 30

<210> 595

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 595

agctagctca gtcctaggga ttatgctagc 30

<210> 596

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 596

agctagctca gtcctaggta ttgtgctagc 30

<210> 597

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 597

ggctagctca gtcctaggta ctatgctagc 30

<210> 598

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 598

ggctagctca gtcctaggta tagtgctagc 30

<210> 599

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 599

ggctagctca gccctaggta ttatgctagc 30

<210> 600

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 600

agctagctca gtcctaggta taatgctagc 30

<210> 601

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 601

agctagctca gtcctaggga ctgtgctagc 30

<210> 602

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 602

ggctagctca gtcctaggta caatgctagc 30

<210> 603

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 603

ggctagctca gtcctaggta tagtgctagc 30

<210> 604

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 604

agctagctca gtcctaggga ttatgctagc 30

<210> 605

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 605

ggctagctca gtcctaggga ttatgctagc 30

<210> 606

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 606

ggctagctca gtcctaggta caatgctagc 30

<210> 607

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 607

agctagctca gcccttggta caatgctagc 30

<210> 608

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 608

agctagctca gtcctaggga ctatgctagc 30

<210> 609

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 609

agctagctca gtcctaggga ttgtgctagc 30

<210> 610

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 610

ggctagctca gtcctaggta ttgtgctagc 30

<210> 611

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子家族成员

<400> 611

agctagctca gtcctaggta taatgctagc 30

<210> 612

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 来自J23107的1bp突变体

<400> 612

ggctagctca gtcctaggta ttatgctagc 30

<210> 613

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 来自J23114的1bp突变体

<400> 613

ggctagctca gtcctaggta caatgctagc 30

<210> 614

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pBAD反向

<400> 614

aaagtgtgac gccgtgcaaa taatcaatgt 30

<210> 615

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> NikR启动子，阻遏表达的转录因子的带状螺旋-螺旋家族的蛋白质

<400> 615

gacgaatact taaaatcgtc atacttattt 30

<210> 616

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> lacq_启动子

<400> 616

aaacctttcg cggtatggca tgatagcgcc 30

<210> 617

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> lacIQ -启动子序列

<400> 617

tgatagcgcc cggaagagag tcaattcagg 30

<210> 618

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 大肠杆菌CreABCD磷酸传感操纵子启动子

<400> 618

ttatttaccg tgacgaacta attgctcgtg 30

<210> 619

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> GlnRS启动子

<400> 619

catacgccgt tatacgttgt ttacgctttg 30

<210> 620

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> lacZ的组成型弱的启动子

<400> 620

ttatgcttcc ggctcgtatg ttgtgtggac 30

<210> 621

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 突变的LacZ启动子

<400> 621

ttatgcttcc ggctcgtatg gtgtgtggac 30

<210> 622

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有(TA)10的组成型启动子

<400> 622

atatatatat atatataatg gaagcgtttt 30

<210> 623

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有(TA)9的组成型启动子

<400> 623

atatatatat atatataatg gaagcgtttt 30

<210> 624

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有(C)10的组成型启动子

<400> 624

ccccgaaagc ttaagaatat aattgtaagc 30

<210> 625

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有(C)12的组成型启动子

<400> 625

ccccgaaagc ttaagaatat aattgtaagc 30

<210> 626

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有13 bp的优化的(TA)重复组成型启动子

<400> 626

tgacaatata tatatatata taatgctagc 30

<210> 627

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有15 bp的优化的(TA)重复组成型启动子

<400> 627

acaatatata tatatatata taatgctagc 30

<210> 628

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有17 bp的优化的(TA)重复组成型启动子

<400> 628

aatatatata tatatatata taatgctagc 30

<210> 629

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有19 bp的优化的(TA)重复组成型启动子

<400> 629

tatatatata tatatatata taatgctagc 30

<210> 630

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有21 bp的优化的(TA)重复组成型启动子

<400> 630

tatatatata tatatatata taatgctagc 30

<210> 631

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有17 bp的优化的(A)重复组成型启动子

<400> 631

aaaaaaaaaa aaaaaaaata taatgctagc 30

<210> 632

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 在-10至-35元件具有18 bp的优化的(A)重复组成型启动子

<400> 632

aaaaaaaaaa aaaaaaaata taatgctagc 30

<210> 633

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> J23101:GFP

<400> 633

caccttcggg tgggcctttc tgcgtttata 30

<210> 634

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> J23119:IFP

<400> 634

caccttcggg tgggcctttc tgcgtttata 30

<210> 635

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> J23119:HO1

<400> 635

caccttcggg tgggcctttc tgcgtttata 30

<210> 636

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 红外信号报告子(J23119:IFP:J23119:HO1)

<400> 636

caccttcggg tgggcctttc tgcgtttata 30

<210> 637

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 双终止子+组成型启动子

<400> 637

ggctagctca gtcctaggta cagtgctagc 30

<210> 638

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 双终止子+组成型启动子+强的RBS

<400> 638

tgctagctac tagagattaa agaggagaaa 30

<210> 639

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> IPTG诱导的Lac启动子盒

<400> 639

ttgtgagcgg ataacaagat actgagcaca 30

<210> 640

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> IPTG诱导的Lac启动子盒

<400> 640

ttgtgagcgg ataacaagat actgagcaca 30

<210> 641

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> IPTG诱导的Lac启动子盒

<400> 641

ttgtgagcgg ataacaagat actgagcaca 30

<210> 642

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因I启动子

<400> 642

cctgttttta tgttattctc tctgtaaagg 30

<210> 643

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因II启动子

<400> 643

aaatatttgc ttatacaatc ttcctgtttt 30

<210> 644

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因III启动子

<400> 644

gctgataaac cgatacaatt aaaggctcct 30

<210> 645

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因IV启动子

<400> 645

ctcttctcag cgtcttaatc taagctatcg 30

<210> 646

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因V启动子

<400> 646

atgagccagt tcttaaaatc gcataaggta 30

<210> 647

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因VI启动子

<400> 647

ctattgattg tgacaaaata aacttattcc 30

<210> 648

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13K07基因VIII启动子

<400> 648

gtttcgcgct tggtataatc gctgggggtc 30

<210> 649

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> M13110

<400> 649

ctttgcttct gactataata gtcagggtaa 30

<210> 650

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> g3的修饰的启动子序列

<400> 650

aaaccgatac aattaaaggc tcctgctagc 30

<210> 651

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子I

<400> 651

caccacactg atagtgctag tgtagatcac 30

<210> 652

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 组成型启动子II

<400> 652

gccggaataa ctccctataa tgcgccacca 30

<210> 653

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> --指定部分列表--

<400> 653

ttgacaagct tttcctcagc tccgtaaact 30

<210> 654

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 全长稳定期osmY启动子

<400> 654

ggtttcaaaa ttgtgatcta tatttaacaa 30

<210> 655

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 最小稳定期osmY启动子

<400> 655

ggtttcaaaa ttgtgatcta tatttaacaa 30

<210> 656

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> htpG热休克启动子

<400> 656

tctattccaa taaagaaatc ttcctgcgtg 30

<210> 657

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子veg，枯草芽孢杆菌的组成型启动子

<400> 657

aaaaatgggc tcgtgttgta caataaatgt 30

<210> 658

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子43，枯草芽孢杆菌的组成型启动子

<400> 658

aaaaaaagcg cgcgattatg taaaatataa 30

<210> 659

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 枯草芽孢杆菌的强的组成型启动子

<400> 659

aattgcagta ggcatgacaa aatggactca 30

<210> 660

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> PliaG

<400> 660

caagcttttc ctttataata gaatgaatga 30

<210> 661

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> PlepA

<400> 661

tctaagctag tgtattttgc gtttaatagt 30

<210> 662

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Pveg

<400> 662

aatgggctcg tgttgtacaa taaatgtagt 30

<210> 663

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>枯草芽孢杆菌的启动子ctc

<400> 663

atccttatcg ttatgggtat tgtttgtaat 30

<210> 664

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>枯草芽孢杆菌的启动子gsiB

<400> 664

taaaagaatt gtgagcggga atacaacaac 30

<210> 665

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>启动子43，枯草芽孢杆菌的组成型启动子

<400> 665

aaaaaaagcg cgcgattatg taaaatataa 30

<210> 666

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 来自沙门氏菌的Pspv2

<400> 666

tacaaaataa ttcccctgca aacattatca 30

<210> 667

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 来自沙门氏菌的Pspv

<400> 667

tacaaaataa ttcccctgca aacattatcg 30

<210> 668

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7启动子(来自T7噬菌体的强启动子)

<400> 668

agggaataca agctacttgt tctttttgca 30

<210> 669

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7启动子

<400> 669

taatacgact cactataggg aga 23

<210> 670

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7启动子

<400> 670

gaatttaata cgactcacta tagggaga 28

<210> 671

<211> 19

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7共有-10和其它

<400> 671

taatacgact cactatagg 19

<210> 672

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 重叠的T7启动子

<400> 672

gagtcgtatt aatacgactc actatagggg 30

<210> 673

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 更多重叠的T7启动子

<400> 673

agtgagtcgt actacgactc actatagggg 30

<210> 674

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 减弱重叠的T7启动子

<400> 674

gagtcgtatt aatacgactc tctatagggg 30

<210> 675

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7共有启动子序列

<400> 675

taatacgact cactataggg aga 23

<210> 676

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7 RNAP启动子

<400> 676

ttatacgact cactataggg aga 23

<210> 677

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7 RNAP启动子

<400> 677

gaatacgact cactataggg aga 23

<210> 678

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7 RNAP启动子

<400> 678

taatacgtct cactataggg aga 23

<210> 679

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7 RNAP启动子

<400> 679

tcatacgact cactataggg aga 23

<210> 680

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7强启动子

<400> 680

taatacgact cactataggg agaccacaac 30

<210> 681

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7弱的结合和持续合成能力

<400> 681

taattgaact cactaaaggg agaccacagc 30

<210> 682

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> T7弱的结合启动子

<400> 682

cgaagtaata cgactcacta ttagggaaga 30

<210> 683

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pCyc (中等)启动子

<400> 683

acaaacacaa atacacacac taaattaata 30

<210> 684

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pAdh (强)启动子

<400> 684

ccaagcatac aatcaactat ctcatataca 30

<210> 685

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pSte5 (弱)启动子

<400> 685

gatacaggat acagcggaaa caacttttaa 30

<210> 686

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> 酵母ADH1启动子

<400> 686

tttcaagcta taccaagcat acaatcaact 30

<210> 687

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> cyc100最小启动子

<400> 687

cctttgcagc ataaattact atacttctat 30

<210> 688

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> cyc70最小启动子

<400> 688

cctttgcagc ataaattact atacttctat 30

<210> 689

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> cyc43最小启动子

<400> 689

cctttgcagc ataaattact atacttctat 30

<210> 690

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> cyc28最小启动子

<400> 690

cctttgcagc ataaattact atacttctat 30

<210> 691

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> cyc16最小启动子

<400> 691

cctttgcagc ataaattact atacttctat 30

<210> 692

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pPGK1

<400> 692

ttatctactt tttacaacaa atataaaaca 30

<210> 693

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> pCYC酵母启动子

<400> 693

acaaacacaa atacacacac taaattaata 30

<210> 694

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>酵母GPD (TDH3)启动子

<400> 694

gtttcgaata aacacacata aacaaacaaa 30

<210> 695

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>酵母中等长度ADH1启动子

<400> 695

ccaagcatac aatcaactat ctcatataca 30

<210> 696

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223>酵母CLB1启动子区，G2/M细胞周期特异的

<400> 696

accatcaaag gaagctttaa tcttctcata 30

<210> 697

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> CMV启动子

<400> 697

agaacccact gcttactggc ttatcgaaat 30

<210> 698

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<220>

<223> Ubc启动子

<400> 698

ggccgttttt ggcttttttg ttagacgaag 30

<210> 699

<211> 9

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 699

Asn Ile Pro Gln Leu Thr Pro Thr Pro

1 5

<210> 700

<211> 27

<212> DNA

<213> 弯曲乳杆菌(Lactobacillus curvatus)

<400> 700

aacattccgc agctgacccc gaccccg 27

<210> 701

<211> 18

<212> PRT

<213> 苏云金芽孢杆菌(Bacillus thuringiensis)

<220>

<221> VARIANT

<222> 4, 7, 10

<223> Xaa =任意氨基酸

<400> 701

Asp Trp Thr Xaa Trp Ser Xaa Leu Val Xaa Ala Ala Cys Ser Val Glu

1 5 10 15

Leu Leu

<210> 702

<211> 54

<212> DNA

<213> 苏云金芽孢杆菌(Bacillus thuringiensis)

<220>

<221> misc_feature

<222> 10, 11, 12, 19, 20, 21, 28, 29, 30

<223> n = A、T、C或G

<400> 702

gattggaccn nntggagcnn nctggtgnnn gcggcgtgca gcgtggaact gctg 54

<210> 703

<211> 30

<212> PRT

<213> 弯曲乳杆菌(Lactobacillus curvatus) L442

<400> 703

Ala Tyr Pro Gly Asn Gly Val His Cys Gly Lys Tyr Ser Cys Thr Val

1 5 10 15

Asp Lys Gln Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala

20 25 30

<210> 704

<211> 90

<212> DNA

<213> 弯曲乳杆菌(Lactobacillus curvatus) L442

<400> 704

gcgtatccgg gcaacggcgt gcattgcggc aaatatagct gcaccgtgga taaacagacc 60

gcgattggca acattggcaa caacgcggcg 90

<210> 705

<211> 43

<212> PRT

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 705

Thr Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Asn Ser Lys Lys Cys Trp

1 5 10 15

Val Asp Trp Gly Thr Ala Gln Gly Cys Ile Asp Val Val Ile Gly Gln

20 25 30

Leu Gly Gly Gly Ile Pro Gly Lys Gly Lys Cys

35 40

<210> 706

<211> 129

<212> DNA

<213> 广布肉杆菌(Carnobacterium divergens)

<400> 706

accaaatatt atggcaacgg cgtgtattgc aacagcaaaa aatgctgggt ggattggggc 60

accgcgcagg gctgcattga tgtggtgatt ggccagctgg gcggcggcat tccgggcaaa 120

ggcaaatgc 129

<210> 707

<211> 24

<212> PRT

<213> 小双孢菌(菌株107891) (Microbispora sp. (strain 107891))

<400> 707

Val Thr Ser Trp Ser Leu Cys Thr Pro Gly Cys Thr Ser Pro Gly Gly

1 5 10 15

Gly Ser Asn Cys Ser Phe Cys Cys

20

<210> 708

<211> 72

<212> DNA

<213> 小双孢菌(菌株107891) (Microbispora sp. (strain 107891))

<400> 708

gtgaccagct ggagcctgtg caccccgggc tgcaccagcc cgggcggcgg cagcaactgc 60

agcttttgct gc 72

<210> 709

<211> 62

<212> PRT

<213> 肠球菌(Enterococcus sp.)

<400> 709

Asn Arg Trp Tyr Cys Asn Ser Ala Ala Gly Gly Val Gly Gly Ala Ala

1 5 10 15

Val Cys Gly Leu Ala Gly Tyr Val Gly Glu Ala Lys Glu Asn Ile Ala

20 25 30

Gly Glu Val Arg Lys Gly Trp Gly Met Ala Gly Gly Phe Thr His Asn

35 40 45

Lys Ala Cys Lys Ser Phe Pro Gly Ser Gly Trp Ala Ser Gly

50 55 60

<210> 710

<211> 186

<212> DNA

<213> 肠球菌(Enterococcus sp.)

<400> 710

aaccgctggt attgcaacag cgcggcgggc ggcgtgggcg gcgcggcggt gtgcggcctg 60

gcgggctatg tgggcgaagc gaaagaaaac attgcgggcg aagtgcgcaa aggctggggc 120

atggcgggcg gctttaccca taacaaagcg tgcaaaagct ttccgggcag cggctgggcg 180

agcggc 186

<210> 711

<211> 39

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 711

Thr Thr Lys Asn Tyr Gly Asn Gly Val Cys Asn Ser Val Asn Trp Cys

1 5 10 15

Gln Cys Gly Asn Val Trp Ala Ser Cys Asn Leu Ala Thr Gly Cys Ala

20 25 30

Ala Trp Leu Cys Lys Leu Ala

35

<210> 712

<211> 117

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 712

accaccaaaa actatggcaa cggcgtgtgc aacagcgtga actggtgcca gtgcggcaac 60

gtgtgggcga gctgcaacct ggcgaccggc tgcgcggcgt ggctgtgcaa actggcg 117

<210> 713

<211> 30

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 713

Ala Ser Ile Ile Lys Thr Thr Ile Lys Val Ser Lys Ala Val Cys Lys

1 5 10 15

Thr Leu Thr Cys Ile Cys Thr Gly Ser Cys Ser Asn Cys Lys

20 25 30

<210> 714

<211> 90

<212> DNA

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 714

gcgagcatta ttaaaaccac cattaaagtg agcaaagcgg tgtgcaaaac cctgacctgc 60

atttgcaccg gcagctgcag caactgcaaa 90

<210> 715

<211> 31

<212> PRT

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 715

Ser Ala Ser Ile Val Lys Thr Thr Ile Lys Ala Ser Lys Lys Leu Cys

1 5 10 15

Arg Gly Phe Thr Leu Thr Cys Gly Cys His Phe Thr Gly Lys Lys

20 25 30

<210> 716

<211> 93

<212> DNA

<213> 表皮葡萄球菌(Staphylococcus epidermidis)

<400> 716

agcgcgagca ttgtgaaaac caccattaaa gcgagcaaaa aactgtgccg cggctttacc 60

ctgacctgcg gctgccattt taccggcaaa aaa 93

<210> 717

<211> 58

<212> PRT

<213> 屎肠球菌(Enterococcus faecium)

<400> 717

Met Glu Lys Leu Thr Val Lys Glu Met Ser Gln Val Val Gly Gly Lys

1 5 10 15

Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val Asp

20 25 30

Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ala Ala Ala Asn Leu

35 40 45

Thr Thr Gly Gly Lys Ala Gly Trp Lys Gly

50 55

<210> 718

<211> 178

<212> DNA

<213> 屎肠球菌(Enterococcus faecium)

<400> 718

atggaaaaat taactgtgaa agaaatgtcg caagtagttg gcggaaagta ctatggtaac 60

ggagtatcat gtaataaaaa gggatgtagt gttgattggg gaaaagctat tggtattatt 120

ggaaataatg ctgctgctaa tttaactact ggcggaaaag cagggtggaa aggttaac 178

<210> 719

<211> 39

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 719

Ala Thr Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Gln Lys His Tyr

1 5 10 15

Thr Trp Val Asp Trp Asn Lys Ala Ser Arg Glu Ile Gly Lys Ile Thr

20 25 30

Val Asn Gly Trp Val Gln His

35

<210> 720

<211> 93

<212> DNA

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 720

agcgcgagca ttgtgaaaac caccattaaa gcgagcaaaa aactgtgccg cggctttacc 60

ctgacctgcg gctgccattt taccggcaaa aaa 93

<210> 721

<211> 35

<212> PRT

<213> 环状芽孢杆菌(Bacillus circulans)

<400> 721

Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys Ser Val

1 5 10 15

Asn Trp Gly Ile Ile Thr His Gln Ala Phe Arg Val Thr Ser Gly Val

20 25 30

Ala Ser Gly

35

<210> 722

<211> 105

<212> DNA

<213> 环状芽孢杆菌(Bacillus circulans)

<400> 722

gtgaactatg gcaacggcgt gagctgcagc aaaaccaaat gcagcgtgaa ctggggcatt 60

attacccatc aggcgtttcg cgtgaccagc ggcgtggcga gcggc 105

<210> 723

<211> 30

<212> PRT

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 723

Phe Val Tyr Gly Asn Gly Val Thr Ser Ile Leu Val Gln Ala Gln Phe

1 5 10 15

Leu Val Asn Gly Gln Arg Arg Phe Phe Tyr Thr Pro Asp Lys

20 25 30

<210> 724

<211> 90

<212> DNA

<213> 多粘类芽孢杆菌(Paenibacillus polymyxa)

<400> 724

tttgtgtatg gcaacggcgt gaccagcatt ctggtgcagg cgcagtttct ggtgaacggc 60

cagcgccgct ttttttatac cccggataaa 90

<210> 725

<211> 13

<212> PRT

<213> 鼠李糖乳杆菌(Lactobacillus rhamnosus)

<400> 725

Ala Val Pro Ala Val Arg Lys Thr Asn Glu Thr Leu Asp

1 5 10

<210> 726

<211> 39

<212> DNA

<213> 鼠李糖乳杆菌(Lactobacillus rhamnosus)

<400> 726

gcggtgccgg cggtgcgcaa aaccaacgaa accctggat 39

<210> 727

<211> 60

<212> PRT

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<400> 727

Met Lys Asn Ser Ala Ala Arg Glu Ala Phe Lys Gly Ala Asn His Pro

1 5 10 15

Ala Gly Met Val Ser Glu Glu Glu Leu Lys Ala Leu Val Gly Gly Asn

20 25 30

Asp Val Asn Pro Glu Thr Thr Pro Ala Thr Thr Ser Ser Trp Thr Cys

35 40 45

Ile Thr Ala Gly Val Thr Val Ser Ala Ser Leu Cys

50 55 60

<210> 728

<211> 180

<212> DNA

<213> 地衣芽孢杆菌(Bacillus licheniformis)

<400> 728

atgaaaaaca gcgcggcgcg cgaagcgttt aaaggcgcga accatccggc gggcatggtg 60

agcgaagaag aactgaaagc gctggtgggc ggcaacgatg tgaacccgga aaccaccccg 120

gcgaccacca gcagctggac ctgcattacc gcgggcgtga ccgtgagcgc gagcctgtgc 180

<210> 729

<211> 689

<212> PRT

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 729

Met Ala Val Asn Asp Tyr Glu Pro Gly Ser Met Val Ile Thr His Val

1 5 10 15

Gln Gly Gly Gly Arg Asp Ile Ile Gln Tyr Ile Pro Ala Arg Ser Ser

20 25 30

Tyr Gly Thr Pro Pro Phe Val Pro Pro Gly Pro Ser Pro Tyr Val Gly

35 40 45

Thr Gly Met Gln Glu Tyr Arg Lys Leu Arg Ser Thr Leu Asp Lys Ser

50 55 60

His Ser Glu Leu Lys Lys Asn Leu Lys Asn Glu Thr Leu Lys Glu Val

65 70 75 80

Asp Glu Leu Lys Ser Glu Ala Gly Leu Pro Gly Lys Ala Val Ser Ala

85 90 95

Asn Asp Ile Arg Asp Glu Lys Ser Ile Val Asp Ala Leu Met Asp Ala

100 105 110

Lys Ala Lys Ser Leu Lys Ala Ile Glu Asp Arg Pro Ala Asn Leu Tyr

115 120 125

Thr Ala Ser Asp Phe Pro Gln Lys Ser Glu Ser Met Tyr Gln Ser Gln

130 135 140

Leu Leu Ala Ser Arg Lys Phe Tyr Gly Glu Phe Leu Asp Arg His Met

145 150 155 160

Ser Glu Leu Ala Lys Ala Tyr Ser Ala Asp Ile Tyr Lys Ala Gln Ile

165 170 175

Ala Ile Leu Lys Gln Thr Ser Gln Glu Leu Glu Asn Lys Ala Arg Ser

180 185 190

Leu Glu Ala Glu Ala Gln Arg Ala Ala Ala Glu Val Glu Ala Asp Tyr

195 200 205

Lys Ala Arg Lys Ala Asn Val Glu Lys Lys Val Gln Ser Glu Leu Asp

210 215 220

Gln Ala Gly Asn Ala Leu Pro Gln Leu Thr Asn Pro Thr Pro Glu Gln

225 230 235 240

Trp Leu Glu Arg Ala Thr Gln Leu Val Thr Gln Ala Ile Ala Asn Lys

245 250 255

Lys Lys Leu Gln Thr Ala Asn Asn Ala Leu Ile Ala Lys Ala Pro Asn

260 265 270

Ala Leu Glu Lys Gln Lys Ala Thr Tyr Asn Ala Asp Leu Leu Val Asp

275 280 285

Glu Ile Ala Ser Leu Gln Ala Arg Leu Asp Lys Leu Asn Ala Glu Thr

290 295 300

Ala Arg Arg Lys Glu Ile Ala Arg Gln Ala Ala Ile Arg Ala Ala Asn

305 310 315 320

Thr Tyr Ala Met Pro Ala Asn Gly Ser Val Val Ala Thr Ala Ala Gly

325 330 335

Arg Gly Leu Ile Gln Val Ala Gln Gly Ala Ala Ser Leu Ala Gln Ala

340 345 350

Ile Ser Asp Ala Ile Ala Val Leu Gly Arg Val Leu Ala Ser Ala Pro

355 360 365

Ser Val Met Ala Val Gly Phe Ala Ser Leu Thr Tyr Ser Ser Arg Thr

370 375 380

Ala Glu Gln Trp Gln Asp Gln Thr Pro Asp Ser Val Arg Tyr Ala Leu

385 390 395 400

Gly Met Asp Ala Ala Lys Leu Gly Leu Pro Pro Ser Val Asn Leu Asn

405 410 415

Ala Val Ala Lys Ala Ser Gly Thr Val Asp Leu Pro Met Arg Leu Thr

420 425 430

Asn Glu Ala Arg Gly Asn Thr Thr Thr Leu Ser Val Val Ser Thr Asp

435 440 445

Gly Val Ser Val Pro Lys Ala Val Pro Val Arg Met Ala Ala Tyr Asn

450 455 460

Ala Thr Thr Gly Leu Tyr Glu Val Thr Val Pro Ser Thr Thr Ala Glu

465 470 475 480

Ala Pro Pro Leu Ile Leu Thr Trp Thr Pro Ala Ser Pro Pro Gly Asn

485 490 495

Gln Asn Pro Ser Ser Thr Thr Pro Val Val Pro Lys Pro Val Pro Val

500 505 510

Tyr Glu Gly Ala Thr Leu Thr Pro Val Lys Ala Thr Pro Glu Thr Tyr

515 520 525

Pro Gly Val Ile Thr Leu Pro Glu Asp Leu Ile Ile Gly Phe Pro Ala

530 535 540

Asp Ser Gly Ile Lys Pro Ile Tyr Val Met Phe Arg Asp Pro Arg Asp

545 550 555 560

Val Pro Gly Ala Ala Thr Gly Lys Gly Gln Pro Val Ser Gly Asn Trp

565 570 575

Leu Gly Ala Ala Ser Gln Gly Glu Gly Ala Pro Ile Pro Ser Gln Ile

580 585 590

Ala Asp Lys Leu Arg Gly Lys Thr Phe Lys Asn Trp Arg Asp Phe Arg

595 600 605

Glu Gln Phe Trp Ile Ala Val Ala Asn Asp Pro Glu Leu Ser Lys Gln

610 615 620

Phe Asn Pro Gly Ser Leu Ala Val Met Arg Asp Gly Gly Ala Pro Tyr

625 630 635 640

Val Arg Glu Ser Glu Gln Ala Gly Gly Arg Ile Lys Ile Glu Ile His

645 650 655

His Lys Val Arg Ile Ala Asp Gly Gly Gly Val Tyr Asn Met Gly Asn

660 665 670

Leu Val Ala Val Thr Pro Lys Arg His Ile Glu Ile His Lys Gly Gly

675 680 685

Lys

<210> 730

<211> 2070

<212> DNA

<213> 铜绿假单胞菌(Pseudomonas aeruginosa)

<400> 730

atggctgtca atgattacga acctggttcg atggttatta cacatgtgca gggtggtggg 60

cgtgacataa tccagtatat tcctgctcga tcaagctacg gtactccacc atttgtccca 120

ccaggaccaa gtccgtatgt cggtactgga atgcaggagt acaggaagct aagaagtacg 180

cttgataagt cccattcaga actcaagaaa aacctgaaaa atgaaaccct gaaggaggtt 240

gatgaactca agagtgaagc ggggttgcca ggtaaagcgg tcagtgccaa tgacatccgc 300

gatgaaaaga gtatcgttga tgcactcatg gatgccaaag caaaatcgct aaaggccatt 360

gaggatcgcc cggccaatct ttatacggct tcagactttc ctcagaagtc agagtcgatg 420

taccagagtc agttgctggc cagccgaaaa ttctatggag agttcctgga tcgccatatg 480

agtgagctgg ccaaagcgta cagcgccgat atctataagg cgcaaatcgc tatcttgaaa 540

caaacgtctc aagagctgga gaataaagcc cggtcattgg aagcagaagc ccagcgagcc 600

gctgctgagg tggaggcgga ctacaaggcc aggaaggcaa atgtcgagaa aaaagtgcag 660

tccgagcttg accaggctgg gaatgctttg cctcaactga ccaatccaac gccagagcag 720

tggcttgaac gcgctactca actggttacg caggcgatcg ccaataagaa gaaattgcag 780

actgcaaaca atgccttgat tgccaaggca cccaatgcac tggagaaaca aaaggcaacc 840

tacaacgccg atctcctagt ggatgaaatc gccagcctgc aagcacggct ggacaagctg 900

aacgccgaaa cggcaaggcg caaggaaatc gctcgtcaag cggcgatcag ggctgccaat 960

acttatgcca tgccagccaa tggcagcgtt gtcgccaccg ccgcaggccg gggtctgatc 1020

caggtcgcac aaggcgccgc atcccttgct caagcgatct ccgatgcgat tgccgtcctg 1080

ggccgggtcc tggcttcagc accctcggtg atggccgtgg gctttgccag tctgacctac 1140

tcctcccgga ctgccgagca atggcaggac caaacgcccg atagcgttcg ttacgccctg 1200

ggcatggatg ccgctaaatt ggggcttccc ccaagcgtaa acctgaacgc ggttgcaaaa 1260

gccagcggta ccgtcgatct gccgatgcgc ctgaccaacg aggcacgagg caacacgacg 1320

accctttcgg tggtcagcac cgatggtgtg agcgttccga aagccgttcc ggtccggatg 1380

gcggcctaca atgccacgac aggcctgtac gaggttacgg ttccctctac gaccgcagaa 1440

gcgccgccac tgatcctgac ctggacgccg gcgagtcctc caggaaacca gaacccttcg 1500

agtaccactc cggtcgtacc gaagccggtg ccggtatatg agggagcgac ccttacaccg 1560

gtgaaggcta ccccggaaac ctatcctggg gtgattacac taccggaaga cctgatcatc 1620

ggcttcccgg ccgactcggg gatcaagccg atctatgtga tgttcaggga tccgcgggat 1680

gtacctggtg ctgcgactgg caagggacag cccgtcagcg gtaattggct cggcgccgcc 1740

tctcaaggtg agggggctcc aattccaagc cagattgcgg ataaactacg tggtaagaca 1800

ttcaaaaact ggcgggactt tcgggaacaa ttctggatag ctgtggctaa tgatcctgag 1860

ttaagtaaac agtttaatcc tggtagttta gctgtaatga gagatggagg ggctccttat 1920

gtcagagagt cagaacaggc tggcgggaga ataaagatcg aaatccacca caaggttcga 1980

atagcagatg gaggcggcgt ttacaatatg gggaaccttg ttgcagtaac gccaaaacgt 2040

catatagaaa tccacaaggg agggaagtga 2070

<210> 731

<211> 36

<212> PRT

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 731

Lys Tyr Tyr Gly Asn Gly Leu Ser Cys Ser Lys Lys Gly Cys Thr Val

1 5 10 15

Asn Trp Gly Gln Ala Phe Ser Cys Gly Val Asn Arg Val Ala Thr Ala

20 25 30

Gly His Gly Lys

35

<210> 732

<211> 108

<212> DNA

<213> 植物乳杆菌(Lactobacillus plantarum)

<400> 732

aaatattatg gcaacggcct gagctgcagc aaaaaaggct gcaccgtgaa ctggggccag 60

gcgtttagct gcggcgtgaa ccgcgtggcg accgcgggcc atggcaaa 108

<210> 733

<211> 30

<212> PRT

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 733

Met Lys Thr Ile Leu Arg Phe Val Ala Gly Tyr Asp Ile Ala Ser His

1 5 10 15

Lys Lys Lys Thr Gly Gly Tyr Pro Trp Glu Arg Gly Lys Ala

20 25 30

<210> 734

<211> 93

<212> DNA

<213> 乳酸乳球菌(Lactococcus lactis)

<400> 734

atgaaaacaa tcctacgttt tgttgctggc tacgatattg ctagtcataa aaagaaaact 60

ggcggctatc catgggaacg tggaaaagct taa 93

<210> 735

<211> 24

<212> PRT

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 735

Gly Asn Pro Lys Val Ala His Cys Ala Ser Gln Ile Gly Arg Ser Thr

1 5 10 15

Ala Trp Gly Ala Val Ser Gly Ala

20

<210> 736

<211> 72

<212> DNA

<213> 嗜酸乳杆菌(Lactobacillus acidophilus)

<400> 736

ggcaacccga aagtggcgca ttgcgcgagc cagattggcc gcagcaccgc gtggggcgcg 60

gtgagcggcg cg 72

<210> 737

<211> 53

<212> PRT

<213> 唾液乳杆菌cp400(Lactobacillus salivarius cp400)

<400> 737

Met Phe Phe Asn Phe Met Lys Lys Val Asp Val Lys Lys Asn Phe Gly

1 5 10 15

Tyr Lys Glu Val Ser Arg Lys Asp Leu Ala Lys Val Asn Gly Gly Lys

20 25 30

Arg Lys Lys His Arg Cys Arg Val Tyr Asn Asn Gly Met Pro Thr Gly

35 40 45

Met Tyr Arg Trp Cys

50

<210> 738

<211> 162

<212> DNA

<213> 唾液乳杆菌cp400(Lactobacillus salivarius cp400)

<400> 738

atgtttttta attttatgaa aaaagtagat gtgaagaaga attttggata taaagaagtt 60

tctagaaaag atctagctaa agtaaatggt ggaaagagaa agaaacatcg ttgcagagtt 120

tataataatg gaatgcctac aggaatgtat cgttggtgct aa 162

<210> 739

<211> 5

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 半胱天冬酶2共有序列

<400> 739

Asp Val Ala Asp Leu

1 5

<210> 740

<211> 5

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 半胱天冬酶2共有序列

<400> 740

Asp Val Ala Asp Ile

1 5

<210> 741

<211> 12

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的RAP结合肽，RBP

<400> 741

Phe His Trp Trp Gln Thr Ser Pro Ala His Phe Ser

1 5 10

<210> 742

<211> 12

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的RAP结合肽，RBP

<400> 742

Trp Pro Phe Ala His Trp Pro Trp Gln Tyr Pro Arg

1 5 10

<210> 743

<211> 9

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的AgrC配体

<220>

<221> VARIANT

<222> 5, 9

<223> C5与F9之间的硫代内酯键

<400> 743

Gly Asp Ser Val Cys Ala Ser Tyr Phe

1 5

<210> 744

<211> 7

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的AgrC配体

<220>

<221> VARIANT

<222> 3, 7

<223> C3与F7之间的硫代内酯键

<400> 744

Ser Val Cys Ala Ser Tyr Phe

1 5

<210> 745

<211> 5

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的Cry1Aa配体

<400> 745

Ser Lys Ala Asp Thr

1 5

<210> 746

<211> 5

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的Cry1Aa配体

<400> 746

Ser Lys Pro Ala Asp

1 5

<210> 747

<211> 11

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的Fsr配体

<220>

<221> VARIANT

<222> 3, 11

<223> S3与A11之间的内酯键

<400> 747

Gln Asn Ser Ala Ala Ala Phe Ala Ala Trp Ala

1 5 10

<210> 748

<211> 11

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的Fsr配体

<220>

<221> VARIANT

<222> 3, 11

<223> S3与A11之间的内酯键

<400> 748

Gln Asn Ser Ala Ala Ala Phe Gly Gln Trp Ala

1 5 10

<210> 749

<211> 7

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的AgrC1、AgrC2

<220>

<221> VARIANT

<222> 4, 7

<223> C4与M7之间的硫代内酯键

<400> 749

Tyr Ser Thr Cys Phe Ile Met

1 5

<210> 750

<211> 7

<212> PRT

<213> 人工序列(Artificial Sequence)

<220>

<223> 合成的AgrC1、AgrC2

<220>

<221> VARIANT

<222> 3, 7

<223> C3与M7之间的硫代内酯键

<400> 750

Ser Thr Cys Ala Phe Ile Met

1 5

Claims (35)

1.在微生物群落中原位生产分泌的抗微生物肽的方法，所述方法包括：

将期望的微生物有机体鉴定为所述微生物群落的成员；

将核酸原位施用至所述微生物群落的至少一种期望的微生物有机体，其中所述核酸编码抗微生物肽，所述抗微生物肽不杀死所鉴定的期望的微生物有机体或不阻止所鉴定的期望的微生物有机体的繁殖，

由此所述至少一种期望的微生物有机体被遗传修饰以表达所述抗微生物肽；以及

允许所述遗传修饰的期望的微生物有机体在所述微生物群落中生长，由此所述遗传修饰的期望的微生物有机体分泌所述抗微生物肽。

2.如权利要求1所述的方法，其中从所述施用到所述允许遗传修饰的期望的微生物有机体生长，所述期望的微生物有机体彼此之间的比例基本上保持不变。

3.如权利要求1-2中任一项所述的方法，其中没有将编码抗微生物肽的免疫调节剂的核酸施用至所述至少一种期望的微生物有机体。

4.如权利要求1-3中任一项所述的方法，其中在所述分泌之前，所述微生物群落被一种或多种未知身份的不期望的微生物有机体污染。

5.如权利要求4所述的方法，其中所述抗微生物肽杀死所述一种或多种不期望的微生物有机体或者阻止所述一种或多种不期望的微生物有机体的繁殖。

6.如权利要求1-5中任一项所述的方法，其中所述抗微生物肽不被所述遗传修饰的期望的微生物有机体物种的野生型基因组编码。

7.如权利要求1-6中任一项所述的方法，其中所述抗微生物肽是所述遗传修饰的微生物有机体外源的，或者其中所述抗微生物肽是合成的。

8.如权利要求1-7中任一项所述的方法，其中所述核酸编码两种或更多种不同的抗微生物肽。

9.如权利要求1-8中任一项所述的方法，其中施用所述核酸包括施用编码不同抗微生物肽的两种或更多种不同的核酸。

10.如权利要求8-9中任一项所述的方法，其中一起选择所述不同的抗微生物肽以靶向抗生素抗性感染。

11.如权利要求1-10中任一项所述的方法，其中施用所述核酸包括：

施用包含所述核酸的质粒；或者

施用包含所述核酸的噬菌体。

12.如权利要求1-11中任一项所述的方法，其中所述期望的微生物有机体包括微生物有机体的两种或更多种不同物种，其中将所述核酸施用至所述不同物种中的仅一种或多于一种。

13.如权利要求1-12中任一项所述的方法，其还包括将不同的核酸原位施用至所述微生物群落中不同于所述基因工程化的微生物有机体的微生物有机体，并且其中所述不同的核酸编码与所述核酸不同的抗微生物肽。

14.如权利要求13所述的方法，其中所述不同的核酸与所述核酸同时施用。

15.如权利要求13所述的方法，其中在与所述核酸不同的时间施用所述不同的核酸。

16.如权利要求1-15中任一项所述的方法，其中所述微生物群落由微生物组组成。

17.如权利要求16所述的方法，其中所述微生物组是选自以下的微生物组：胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤。

18.如权利要求1-17中任一项所述的方法，其中所述微生物群落是对象自体的，并且其中离体施用所述核酸，

所述方法还包括将包含所述遗传修饰的期望的微生物有机体的微生物群落施用至所述对象。

19.如权利要求1-18中任一项所述的方法，其中所述微生物群落是保存的健康样品，并且其中在施用时，所述对象患有所述对象的微生物组的失调。

20.如权利要求1-15中任一项所述的方法，其中所述微生物群落由工业培养物组成。

21.微生物群落，其包含：

期望的微生物有机体，其中所述期望的微生物有机体中的至少一种是遗传修饰的，包含编码以下的第一核酸：

第一抗微生物肽，其不靶向所述期望的微生物有机体；和

所述第一抗微生物肽框内的分泌信号；以及

无细胞的第二核酸，其具有与所述第一核酸相同的序列。

22.如权利要求21所述的微生物群落，其中所述第一抗微生物肽不被所述遗传修饰的期望的微生物有机体物种的野生型基因组编码。

23.如权利要求21-22中任一项所述的微生物群落，其中所述第一抗微生物肽是合成的。

24.如权利要求21-22中任一项所述的微生物群落，其中所述第一抗微生物肽是所述遗传修饰的微生物有机体外源的。

25.如权利要求21-24中任一项所述的微生物群落，其中所述期望的微生物有机体不包含所述抗微生物肽的免疫调节剂。

26.如权利要求21-25中任一项所述的微生物群落，其中所述微生物群落被一种或多种未知身份的不期望的微生物有机体污染。

27.如权利要求21-26中任一项所述的微生物群落，其中所述第一核酸编码两种或更多种不同的抗微生物肽。

28.如权利要求21-27中任一项所述的微生物群落，所述遗传修饰的微生物有机体还包含编码以下的第三核酸：

第二抗微生物肽，其不靶向所述一种或多种期望的微生物有机体；以及

所述第二抗微生物肽框内的分泌信号。

29.如权利要求21-28中任一项所述的微生物群落，其中所述无细胞的第二核酸由质粒或噬菌体组成。

30.如权利要求21-29中任一项所述的微生物群落，其中所述期望的微生物有机体包含微生物有机体的两种或更多种不同物种，其中所述第一核酸由所述不同物种中的仅一种或多于一种组成。

31.如权利要求21-30中任一项所述的微生物群落，其中所述微生物群落由微生物组组成。

32.如权利要求31所述的微生物群落，其中所述微生物组是选自以下的微生物组：胃肠道、皮肤、乳腺、胎盘、生物流体、精液、子宫、阴道、卵泡、肺、唾液、口腔、粘膜、结膜、胆道和土壤。

33.如权利要求21-32中任一项所述的微生物群落，其中所述微生物群落在对象体内原位。

34.如权利要求21-32中任一项所述的微生物群落，其中所述微生物群落是对象自体的，以及是所述对象离体的。

35.如权利要求21-30中任一项所述的微生物群落，其中所述微生物群落是工业培养物。