CN116660569A - Multifunctional quantitative liquid dripping workstation - Google Patents
Multifunctional quantitative liquid dripping workstation Download PDFInfo
- Publication number
- CN116660569A CN116660569A CN202310875868.5A CN202310875868A CN116660569A CN 116660569 A CN116660569 A CN 116660569A CN 202310875868 A CN202310875868 A CN 202310875868A CN 116660569 A CN116660569 A CN 116660569A
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- reagent
- mechanical arm
- carbon paper
- liquid
- dropping
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- 239000007788 liquid Substances 0.000 title claims abstract description 80
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 68
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 39
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 39
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- 238000003487 electrochemical reaction Methods 0.000 claims abstract description 19
- 230000007306 turnover Effects 0.000 claims abstract description 7
- 239000002699 waste material Substances 0.000 claims description 19
- 238000004140 cleaning Methods 0.000 claims description 17
- 238000012360 testing method Methods 0.000 claims description 17
- 238000005086 pumping Methods 0.000 claims description 7
- 240000007651 Rubus glaucus Species 0.000 claims description 3
- 235000011034 Rubus glaucus Nutrition 0.000 claims description 3
- 235000009122 Rubus idaeus Nutrition 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000002791 soaking Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000036544 posture Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The application discloses a multifunctional quantitative liquid dropping workstation which comprises a base, a liquid extracting and dropping device, a reagent containing device and an electrochemical reaction device, wherein the liquid extracting and dropping device, the reagent containing device and the electrochemical reaction device are arranged on the base, the liquid extracting and dropping device comprises a mechanical arm and a suction assembly arranged at the working end of the mechanical arm, the reagent containing device is used for containing a reagent to be tested, the electrochemical reaction device comprises a reaction tank for containing the reaction reagent, a carbon paper clamp which is rotatably arranged above the reaction tank and used for fixing carbon paper, and a driving piece which is connected with the carbon paper clamp and used for driving the carbon paper clamp to turn over. The workstation adopts advanced automation technology, can realize high-precision and high-repeatability quantitative liquid dropping operation, can accurately control the dropping speed and volume, can easily perform the liquid dropping operation by a user, does not need manual operation and complicated metering steps, can automatically perform the soaking operation of carbon paper, improves the experimental efficiency, reduces human errors and ensures the reliability of experimental results.
Description
Technical Field
The application relates to the technical field of electrochemical instruments, in particular to a multifunctional quantitative liquid dripping workstation.
Background
In the laboratory, when an electrochemical reaction test is performed, the reagent to be tested needs to be extracted and then dripped on the carbon paper, and then the carbon paper is soaked in an electrochemical reagent pool for reaction. At present, the operations are completed manually through tools such as a liquid-transferring gun, the accuracy and repeatability are difficult to be guaranteed during the operation, the reliability of test results is low, the operation is complex, and the efficiency is low.
Disclosure of Invention
The application aims to provide a multifunctional quantitative liquid dripping workstation which solves the problems that the accuracy and repeatability of the existing electrochemical reaction test operation mode are difficult to guarantee, the operation is complicated and the efficiency is low.
The application realizes the above purpose through the following technical scheme:
the multifunctional quantitative liquid dropping workstation comprises a base, a liquid extracting and dropping device, a reagent containing device and an electrochemical reaction device, wherein the liquid extracting and dropping device, the reagent containing device and the electrochemical reaction device are arranged on the base, the liquid extracting and dropping device comprises a mechanical arm and a suction assembly arranged at the working end of the mechanical arm, the reagent containing device is used for containing a reagent to be tested, the electrochemical reaction device comprises a reaction tank used for containing a reaction reagent, a carbon paper clamp which is rotatably arranged above the reaction tank and used for fixing carbon paper, and a driving piece which is connected with the carbon paper clamp and used for driving the carbon paper clamp to turn over;
when the reagent is dripped, the suction assembly is moved to the reagent containing device through the mechanical arm and the reagent to be detected is extracted, the suction assembly is moved to the electrochemical reaction device through the mechanical arm and the reagent to be detected is dripped on the carbon paper fixed by the carbon paper clamp, and then the carbon paper clamp is driven to turn over through the driving piece, so that the carbon paper is soaked in the reaction reagent in the reaction tank.
The suction assembly comprises a mounting frame, a fixing seat arranged on one side of the mounting frame, a microinjector arranged in the fixing seat, a clamp block clamped and fixed on the handle of the microinjector, and a power piece arranged inside the mounting frame and used for driving the clamp block to move along a straight line.
The power piece comprises a motor, a screw rod arranged at the output end of the motor, a movable block connected with the screw rod in a threaded mode, and a guide rod parallel to the screw rod and penetrating through the movable block in a sliding mode, wherein the clamp block is connected with the movable block.
The reagent holding device is further improved in that the reagent holding device comprises a bearing frame, and a plurality of holding holes for holding test tubes are formed in the bearing frame.
The further improvement lies in that every side position of placing the hole all is equipped with the level gauge that is used for real-time detection to correspond the intraductal liquid level height value of test, reagent holds the device and still includes the lifter that is used for driving the carrier and goes up and down to and the controller of control lifter work, the controller is configured as: when the suction component extracts the reagent to be detected, the liquid level value in the corresponding test tube detected by the liquid level meter in real time is obtained, and the lifting piece is controlled to drive the bearing frame to carry out height adjustment according to the liquid level value, so that the needle head of the microinjector is always positioned at a set depth below the liquid level.
A further improvement is that the set depth is 2mm.
The workstation is characterized by further comprising a waste liquid cylinder which is arranged on the base and used for containing waste liquid and a cleaning cylinder which is used for containing cleaning liquid, wherein after the reagent to be tested is dripped on the carbon paper, the suction assembly is moved to the waste liquid cylinder through the mechanical arm and the rest reagent to be tested is dripped into the waste liquid cylinder, the suction assembly is moved to the cleaning cylinder through the mechanical arm and the cleaning liquid is extracted, and then the suction assembly is moved to the waste liquid cylinder through the mechanical arm and the cleaning liquid is dripped into the waste liquid cylinder.
The mechanical arm is a X, Y, Z triaxial mechanical arm, and the main control equipment of the mechanical arm adopts raspberry pie and controls the mechanical arm through a python program in a linux environment.
The application has the beneficial effects that:
(1) The workstation adopts advanced automation technology, can realize high-precision and high-repeatability quantitative liquid dropping operation, can accurately control the dropping speed and volume, can easily perform the liquid dropping operation by a user, does not need manual operation and complicated metering steps, and can automatically perform the soaking operation of carbon paper, thereby not only improving the efficiency of experiments, but also reducing the possibility of human errors and ensuring the reliability of experimental results.
(2) The workstation adopts a specific reagent containing device, and the carrying height of the workstation can be adjusted according to the real-time liquid level in the test tube during the extraction operation, so that the needle head of the microinjector is always positioned at a set depth below the liquid level, and the problem that the reagent is adhered to the outer wall of the needle tube due to the fact that the needle head stretches into the liquid level too much can be avoided by setting the set depth to be a smaller value (for example, 2 mm), because the adhered reagent slides down to pollute a table board during transfer, and the quantitative precision is reduced due to sliding down during liquid dropping; in addition, the problem that the needle head is separated from the liquid level due to insufficient reagent amount can be avoided, and the empty pumping is effectively prevented.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a multifunctional quantitative drip station;
FIG. 2 is a schematic view of the external structure of the suction assembly;
FIG. 3 is a schematic view of the drive configuration of the power member in the pumping assembly;
FIG. 4 is a schematic structural view of an electrochemical reaction apparatus;
FIG. 5 is a control schematic diagram of the controller controlling the lifting member;
in the figure: 1. a base; 2. a liquid droplet extracting device; 21. a mechanical arm; 22. a suction assembly; 221. a mounting frame; 222. a fixing seat; 223. a microinjector; 224. a clamp block; 225. a motor; 226. a screw rod; 227. a movable block; 228. a guide rod; 3. a reagent holding device; 31. a carrier; 32. placing the hole; 33. a liquid level gauge; 34. a lifting member; 35. a controller; 4. an electrochemical reaction device; 41. a reaction tank; 42. a carbon paper clamp; 43. a driving member; 5. a waste liquid cylinder; 6. and (5) cleaning the jar.
Detailed Description
The present application will be described in further detail with reference to the accompanying drawings, wherein it is to be understood that the following detailed description is for the purpose of further illustrating the application only and is not to be construed as limiting the scope of the application, as various insubstantial modifications and adaptations of the application to those skilled in the art can be made in light of the foregoing disclosure.
Referring to fig. 1-4, a multifunctional quantitative drip station comprises a base 1, a drip pumping device 2, a reagent holding device 3 and an electrochemical reaction device 4, wherein the drip pumping device 2 comprises a mechanical arm 21 and a suction component 22 arranged at the working end of the mechanical arm 21, the reagent holding device 3 is used for holding a reagent to be tested, the electrochemical reaction device 4 comprises a reaction tank 41 for holding the reagent to be tested, a carbon paper clamp 42 rotatably arranged above the reaction tank 41 and used for fixing carbon paper, a driving piece 43 connected with the carbon paper clamp 42 and used for driving the carbon paper clamp 42 to turn over, and the driving piece 43;
during dripping, the suction assembly 22 is moved to the reagent containing device 3 through the mechanical arm 21 and the reagent to be detected is extracted, then the suction assembly 22 is moved to the electrochemical reaction device 4 through the mechanical arm 21 and the reagent to be detected is dripped on the carbon paper fixed by the carbon paper clamp 42, then the carbon paper clamp 42 is driven to overturn through the driving piece 43 so that the carbon paper is soaked in the reaction reagent in the reaction tank 41, and the reaction tank 41 is powered on, so that the electrochemical reaction can be completed. The driving piece 43 can adopt a steering engine and is controlled by an STM32 singlechip, after the reagent is dripped on the prepared carbon paper, the singlechip can control the steering engine to turn over, the carbon paper is immersed in the reaction reagent prepared below for reaction, and the steering engine can also be controlled to turn over again for airing operation.
The mechanical arm 21 is a X, Y, Z triaxial mechanical arm, a main control device of the mechanical arm 21 adopts raspberry pie, and the mechanical arm 21 is controlled by a python program in a linux environment, and the X, Y, Z axes and other postures of the mechanical arm are determined in the program. Firstly, downloading an upper computer and configuring an environment. The python program is then written. First, a program is written to move the robot arm 21 to the position 1 where the reagent pack 3 is located. After the mechanical arm is moved to the No. 1 position, the reagent can be extracted. After the operation of the mechanical arm 21 is waited, programming is performed to enable the mechanical arm 21 to move to the position No. 2, wherein the position No. 2 is the position where the electrochemical reaction device 4 is located, the mechanical arm 21 can drop the reagent onto the carbon paper, and after the operation of the mechanical arm 21 is waited, programming is performed to enable the mechanical arm 21 to reset. The above procedure completes the dropping operation of the mechanical arm 21.
In the present application, the suction unit 22 preferably includes a mounting frame 221, a fixing base 222 disposed at one side of the mounting frame 221, a microinjector 223 disposed in the fixing base 222, a clamp block 224 for clamping the shank of the microinjector 223, and a power member disposed inside the mounting frame 221 for driving the clamp block 224 to move along a straight line. The power piece drives the clamp block 224 to move upwards, so that the microinjector 223 can perform extraction action, the power piece drives the clamp block 224 to move downwards, so that the microinjector 223 can perform injection action, and the reagent drip amount can be accurately controlled through upward and downward movement amount.
In the application, the power part preferably comprises a motor 225, a screw rod 226 arranged at the output end of the motor 225, a movable block 227 connected with the screw rod 226 in a threaded manner, and a guide rod 228 parallel to the screw rod 226 and penetrating through the movable block 227 in a sliding manner, wherein the clamp block 224 is connected with the movable block 227. When the clamp is in operation, the motor 225 drives the screw rod 226 to rotate forward and backward, and the movable block 227 and the clamp block 224 can be driven to move up and down.
In the present application, the reagent holding apparatus 3 preferably includes a carrier 31, and a plurality of placing holes 32 for placing test tubes are formed in the carrier 31. In actual operation, the robotic arm 21 can be accurately positioned to the position of each placement hole 32 by programming, so that the dripping operation of different reagents is realized.
In addition, since the height of the needle of the microinjector 223 is fixed during the drawing of the mechanical arm 21, and the amount of the reagent prepared in the test tube is different each time, the condition that the needle stretches into the test tube to be lower than the liquid level of the reagent is likely to occur, so that more reagent is adhered to the outer wall of the needle tube, the adhered reagent slides down during the transferring to pollute the table board, and the quantitative accuracy is also reduced during the dropping; of course, there may be cases where the needle does not extend below the liquid surface, resulting in empty pumping, and no reagent can be extracted.
To this end, the application in a preferred embodiment is modified as follows:
referring to fig. 5, each of the placement holes 32 is provided at a lateral position thereof with a level gauge 33 (e.g., a non-contact level sensor) for detecting a corresponding level value in the test tube in real time, the reagent holding device 3 further includes a lifting member 34 (e.g., an electric telescopic rod, a screw nut pair, etc.) for driving the carrier 31 to lift, and a controller 35 for controlling the operation of the lifting member 34, and the controller 35 is configured to: when the suction assembly 22 extracts the reagent to be detected, the liquid level value in the corresponding test tube detected by the liquid level meter 33 in real time is obtained, and the lifting piece 34 is controlled to drive the bearing frame 31 to adjust the height according to the liquid level value, so that the needle of the micro-syringe 223 is always positioned at a set depth below the liquid level. Preferably, the set depth is 2mm, and the set depth is not too small, so that the doped air is avoided being pumped. For example, the level gauge 33 detects that the liquid level of a reagent in a test tube (the height can be described by taking the table surface of the base 1 as a reference) is 120mm, and the height of the needle of the microinjector 223 is fixed at 100mm, at this time, the liquid level is too high, the needle can be directly drawn to be deep 20mm below the liquid level, so the controller 35 controls the lifting member 34 to drive the carrier 31 to descend 18mm, and then the liquid level also descends 18mm, so the needle can be located at a position with a set depth of 2mm below the liquid level, and as the drawing process progresses, the reagent in the test tube gradually decreases, the liquid level gradually descends, at this time, the controller 35 controls the lifting member 34 to drive the carrier 31 gradually ascends, so that the needle is always located at a position with a set depth of 2mm below the liquid level, and thus the phenomenon that the needle is not separated from the liquid level, and the phenomenon that the needle stretches into the deep will not occur.
In the application, the workstation preferably further comprises a waste liquid cylinder 5 which is arranged on the base 1 and is used for containing waste liquid and a cleaning cylinder 6 which is used for containing cleaning liquid, after the reagent to be tested is dripped on the carbon paper, the suction assembly 22 is moved to the waste liquid cylinder 5 by the mechanical arm 21 and the rest reagent to be tested is dripped into the waste liquid cylinder 5, the suction assembly 22 is moved to the cleaning cylinder 6 by the mechanical arm 21 and the cleaning liquid is pumped, the suction assembly 22 is moved to the waste liquid cylinder 5 by the mechanical arm 21 and the cleaning liquid is dripped into the waste liquid cylinder 5, and then the mechanical arm 21 is reset. The process of extracting the cleaning solution can be repeated for a plurality of times, so as to ensure that the suction assembly 22 is completely cleaned, and avoid interference to the next dripping operation.
The foregoing examples illustrate only a few embodiments of the application and are described in detail herein without thereby limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application.
Claims (8)
1. The multifunctional quantitative liquid dropping workstation is characterized by comprising a base (1), a liquid dropping pumping device (2), a reagent containing device (3) and an electrochemical reaction device (4), wherein the liquid dropping pumping device (2) comprises a mechanical arm (21) and a suction component (22) arranged at the working end of the mechanical arm (21), the reagent containing device (3) is used for containing a reagent to be tested, the electrochemical reaction device (4) comprises a reaction tank (41) used for containing the reagent to be tested, a carbon paper clamp (42) which is rotatably arranged above the reaction tank (41) and used for fixing carbon paper, and a driving piece (43) which is connected with the carbon paper clamp (42) and used for driving the carbon paper clamp (42) to turn over;
during liquid dropping, the suction assembly (22) is moved to the reagent containing device (3) through the mechanical arm (21) and the reagent to be detected is extracted, the suction assembly (22) is moved to the electrochemical reaction device (4) through the mechanical arm (21) and the reagent to be detected is dripped on the carbon paper fixed by the carbon paper clamp (42), and then the carbon paper clamp (42) is driven to overturn through the driving piece (43) so that the carbon paper is soaked in the reaction reagent in the reaction tank (41).
2. The multifunctional quantitative drip station according to claim 1, wherein the suction assembly (22) comprises a mounting frame (221), a fixing seat (222) arranged on one side of the mounting frame (221), a microinjector (223) arranged in the fixing seat (222), a clamp block (224) clamped and fixed on the handle of the microinjector (223), and a power piece arranged inside the mounting frame (221) and used for driving the clamp block (224) to move along a straight line.
3. A multi-functional quantitative drip station according to claim 2, characterized in that the power part comprises a motor (225) and a screw rod (226) arranged at the output end of the motor (225), a movable block (227) connected with the screw rod (226) in a threaded manner, and a guide rod (228) parallel to the screw rod (226) and penetrating the movable block (227) in a sliding manner, wherein the clamp block (224) is connected with the movable block (227).
4. A multi-functional quantitative drip station according to claim 2, characterized in that the reagent holding device (3) comprises a carrier (31), wherein the carrier (31) is provided with a plurality of holding holes (32) for holding test tubes.
5. A multi-functional quantitative drip station according to claim 4, characterized in that the lateral position of each placement hole (32) is provided with a liquid level gauge (33) for detecting the corresponding liquid level value in the test tube in real time, the reagent holding device (3) further comprises a lifting member (34) for driving the carrying frame (31) to lift, and a controller (35) for controlling the operation of the lifting member (34), the controller (35) being configured to: when the suction component (22) extracts the reagent to be detected, the liquid level value in the corresponding test tube detected by the liquid level meter (33) in real time is obtained, and the lifting piece (34) is controlled to drive the bearing frame (31) to carry out height adjustment according to the liquid level value, so that the needle head of the microinjector (223) is always positioned at a set depth below the liquid level.
6. A multi-purpose metering drop station as claimed in claim 5, wherein the set depth is 2mm.
7. The multifunctional quantitative dripping workstation according to claim 1, further comprising a waste liquid cylinder (5) arranged on the base (1) and used for containing waste liquid and a cleaning cylinder (6) used for containing cleaning liquid, wherein after the reagent to be detected is dripped on the carbon paper, the suction assembly (22) is moved to the waste liquid cylinder (5) through the mechanical arm (21) and the rest of the reagent to be detected is dripped in the waste liquid cylinder (5), the suction assembly (22) is moved to the cleaning cylinder (6) through the mechanical arm (21) and the cleaning liquid is extracted, and then the suction assembly (22) is moved to the waste liquid cylinder (5) through the mechanical arm (21) and the cleaning liquid is dripped in the waste liquid cylinder (5).
8. The multifunctional quantitative drip station according to claim 1, wherein the mechanical arm (21) is a X, Y, Z triaxial mechanical arm, the main control equipment of the mechanical arm (21) adopts raspberry pie, and the mechanical arm (21) is controlled by a python program in a linux environment.
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CN202310875868.5A CN116660569B (en) | 2023-07-17 | 2023-07-17 | Multifunctional quantitative liquid dripping workstation |
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CN202310875868.5A CN116660569B (en) | 2023-07-17 | 2023-07-17 | Multifunctional quantitative liquid dripping workstation |
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