CN213295350U - Automatic sample adding platform for biological test - Google Patents

Abstract

The utility model provides an automatic application of sample platform for biological assay, place the district including the EP pipe, be close to the PCR pipe that the EP pipe placed the district and place the district, move liquid suction head box and place the position, touch-sensitive screen, the garbage bin is regional, the gas tightness detects needle and places position and application of sample mechanism, and the setting is placed between liquid suction head box and place the position and the touch-sensitive screen to the gas tightness detection needle, and application of sample mechanism connects on track one, and track one sets up and places the position and the EP pipe is placed between the district at liquid suction head box, and application of sample mechanism is connected with control chip. The utility model provides an automatic application of sample platform is used in biological assay adopts automatic application of sample method in the minizone, realizes partial shipment and application of sample operation process among the biological assay, can operate on the desktop, saves the manual work, has promoted work efficiency to operation mistake and error have been reduced.

Description

Automatic sample adding platform for biological test

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to an automatic application of sample platform for biological assay.

Background

The sample adding operation is a common experimental step in biological tests, and in the experimental process of the prior art, the technology for subpackaging and sample adding operation is divided into two types: firstly, an operation arm is utilized to integrate a sample into the automatic nucleic acid extractor, and the method has the advantages of large equipment volume, high cost and narrow application range and is mainly used for an NGS detection laboratory; secondly, the split charging and sample adding process is completed through pure manual operation, however, the method is high in working intensity, low in working efficiency and prone to error of operators, and meanwhile, different operators have different sampling amounts and uncontrollable errors. Therefore, how to improve the split charging and sample adding operation in the prior art becomes a technical problem which needs to be solved urgently.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an automatic application of sample platform for biological assay has solved how to adopt automatic application of sample method in the minizone, realizes partial shipment and application of sample operation process among the biological assay, can operate on the desktop, saves the manual work, has promoted work efficiency to operation mistake and error have been reduced.

The utility model provides a biological assay is with automatic application of sample platform, includes that the EP pipe is placed the district, is close to the PCR pipe that the EP pipe was placed the district is placed the district, is moved liquid suction head box and is placed position, touch-sensitive screen, garbage bin region, gas tightness detection needle and place position and application of sample mechanism, gas tightness detection needle is placed the position and is set up move liquid suction head box and place between position and the touch-sensitive screen, application of sample mechanism connects on track one, track one sets up and places between the district at the position that moves liquid suction head box and EP pipe, application of sample mechanism is connected with control chip.

The sample adding mechanism comprises an upright support arranged on the first rail, a horizontal shaft arranged at the top end of the upright support and a liquid taking device, the upright support is arranged on the first rail in a sliding mode through a driving motor, a second rail is arranged on the horizontal shaft along the length direction of the horizontal shaft, and the liquid taking device is arranged on the second rail in a sliding mode.

The cross shaft is fixedly connected with the upright post bracket, and a specific motor driving mechanism is the prior art, specifically, a driving motor is coaxially connected with a gear through the meshing of the gear and a rack, the gear rotates through the rotation of a rotating shaft, so that the upright post bracket or the liquid taking device moves along a track, wherein the rack is arranged along the length direction of the track, and the prior art is not detailed in detail herein.

Get the liquid ware include the connecting block, with the flexible pipe of getting liquid, setting that the connecting block is connected are in motor one and motor two, the attachment at connecting block both ends get motor three, sleeve, air pump on the flexible pipe of liquid and with the connecting pipe that the air pump is connected, the connecting block passes through a motor slidable sets up on the cross axle, get the liquid ware essence promptly and set up on track two through the connecting block, get the flexible pipe of liquid and pass through motor two with the connecting block is connected, it passes through to get the flexible pipe of liquid three slidable settings of motor are in the sleeve, it is provided with on the flexible pipe of liquid to get the air pump.

The second motor is used for enabling the sleeve and the liquid taking telescopic pipe to move up and down relatively, and comprises a loading liquid-transferring suction head, a liquid taking device and a PCR (polymerase chain reaction) pipe, wherein the loading liquid-transferring suction head extends into the EP pipe to take liquid, and then the loading liquid-transferring suction head extends into the PCR pipe to perform sample adding work; the third motor is used for driving the sleeve to move up and down so as to adjust the relative position between the sleeve and the liquid taking telescopic pipe.

The working principle of the first motor, the second motor and the third motor in the patent is consistent with that of the driving motor, and the linear reciprocating motion is realized by adopting the principle of meshing the gear and the rack, so that the detailed description is omitted.

The liquid taking telescopic pipe is used up and down in a reciprocating way, and the movement range is within 0-9 cm;

the sleeve is a barrel which is wholly wrapped on the periphery of the suction head, the inner diameter of the sleeve is 0.2cm larger than the outer diameter of the suction head, the sleeve can move relative to the liquid taking telescopic pipe under the action of the motor III, and after the liquid taking telescopic pipe absorbs the air tightness detection needle, the sleeve and the liquid taking telescopic pipe move relative to each other, so that the air tightness detection needle is ejected out.

The bottom of the air tightness detection needle is designed in a sealing mode, and the external shape of the air tightness detection needle is consistent with that of a common liquid transfer suction head.

And the EP tube placing area and the PCR tube placing area are provided with refrigerating devices.

The refrigeration temperature can not be below zero, and generally 2-8 ℃ is required.

The utility model discloses reach following effect of showing:

(1) compared with the traditional manual operation, the operation error is reduced, and the working efficiency is increased;

(2) compare current application of sample equipment, small realization desktop formula operation, the replacement manual work of simple does not have peculiar professional equipment and consumptive material, does benefit to equipment application and popularization.

Drawings

Fig. 1 is a top view of the sample adding platform in the embodiment of the present invention.

Fig. 2 is a sectional view of a robot arm according to an embodiment of the present invention.

Fig. 3 is a structural diagram of the air tightness detecting pin according to the embodiment of the present invention.

Fig. 4 is a sectional view of the liquid extractor in the embodiment of the present invention.

Wherein the reference numerals are: 1. a first track; 2. a connecting site; 2-1, upright post bracket; 2-2, horizontal axis; 2-3, track two; 2-4, taking a liquid device; 2-41, connecting blocks; 2-42, motor one; 2-43, motor two; 2-44 and motor three; 2-45, sleeve; 2-46, a liquid taking telescopic pipe; 2-47, piston air pump; 2-48, connecting pipe; 2-5, track three; 2-6, driving a motor; 3. placing a liquid-transfering sucker box; 4. an EP tube placement area; 5. a PCR reaction tube; 6. a trash can area; 7. a touch screen; 8. detecting a needle placement position; 8-1, and an air tightness detection needle.

Detailed Description

In order to clearly illustrate the technical features of the present solution, the present solution is described below by way of specific embodiments.

The equipment of this patent divides into 7 functional areas altogether:

as shown in fig. 1, the position No. 1 is a first robot arm operation track, and is used for the robot arm to move back and forth along the track under the control of a chip, so as to achieve the movement of the robot arm in the X-axis direction;

the position No. 2 is a connecting part for operating the mechanical arm, and is used for being connected with the base shown in the figure 2 and driving the equipment in the figure 2 to reciprocate along the first track shown in the figure 1;

the No. 3 position is a liquid-transferring sucker box placing position which is used for placing a liquid-transferring sucker box and can be used for loading liquid-transferring suckers;

the No. 4 position is a 1.5mL EP tube placing area, an EP tube for large package of reaction liquid or nucleic acid storage liquid is placed at the EP tube, a mechanical arm sucks the liquid for transfer and subpackage, each hole of the area has a unique number (1A, 1B …, 2A,2B …) for 30 positions in total, the reagent or nucleic acid placing position can be conveniently selected in the area of the touch screen 7 in the figure 1, a refrigerating device is arranged in the area, and the 4 ℃ environment of the area is maintained during startup operation;

the position 5 is a position for placing a PCR reaction tube (8 continuous strips or a 96-well plate) and is used for placing the PCR reaction tube and receiving liquid transferred by a mechanical arm from an EP tube placing area in the figure 1, each hole in the area has a unique number (A1, A2 …, B1 and B2 …) for 96 positions in total, so that a sample adding or subpackaging position is conveniently selected in the area of the touch screen 7 in the figure 1, a refrigerating device is arranged in the area, and the environment of 4 ℃ in the area is maintained during startup operation;

the No. 6 position is a garbage can area and is used for placing medical waste garbage bags and collecting and temporarily storing the liquid transfer suction heads unloaded from the liquid transfer telescopic pipes 2-46;

the No. 7 position is a touch screen and is used for distributing the sampling of a mechanical arm from a certain hole at the No. 4 position and adding the sample into a certain hole or a certain hole at the No. 5 position so as to realize automatic sample adding;

and the position 8 is a placing position of the air tightness detection needle, before operation every time, the detection needle is loaded, and the negative pressure is maintained for 10s so as to detect whether the air tightness of the liquid taking device is intact.

Fig. 2 is a cross-sectional view of the manipulator arm of fig. 1:

the mark 2-1 is a stand column bracket of the operation arm, and is connected with the first rail in the figure 1 and can drive the mechanical arm to complete the movement in the X-axis direction;

reference numeral 2-2 is a transverse axis of the robot arm, which supports the apparatus shown in FIG. 4 to move in the Y-axis direction;

the label 2-3 is a second rail on the horizontal axis in fig. 2, which is used for guiding the apparatus in fig. 4 to move back and forth along the second rail to complete the movement in the Y-axis direction;

the labels 2-4 are liquid extractors, the specific structure is shown in detail in fig. 4, a liquid transfer suction head can be inserted and extracted, then the liquid transfer suction head is moved to an EP tube placing area to absorb corresponding reaction liquid/nucleic acid, then the liquid transfer suction head is moved to a PCR reaction tube area, liquid is added into the corresponding reaction hole, finally the liquid transfer suction head is moved to a garbage can area, and the used liquid transfer suction head is discarded into the garbage can to complete the whole operation process.

And the air tightness detection needle 8-1 is similar to a common liquid transfer suction head in appearance, but the bottom of the air tightness detection needle is designed in a sealing mode so as to maintain the negative pressure of the mechanical arm and detect whether the contact surface of the mechanical arm and the liquid transfer suction head is sealed fully or not.

As shown in fig. 4, a cross-sectional view of the liquid extractor is shown:

reference numerals 2-41 denote connecting blocks, the device connects the liquid taking device shown in fig. 4 with a transverse shaft of the mechanical arm shown in fig. 2, and the device is provided with two electric motors (comprising a first motor and a second motor) shown in fig. 4;

the marks 2-42 are first motors, and the device can drive the whole body of the figure 4 to move along a second track shown in the figure 2 to complete the motion in the Y-axis direction;

as shown in fig. 4, the reference 2-44 is a third motor, and the device is attached to the liquid taking telescopic tube shown in fig. 4, and moves along the track three times in a small range to complete the relative movement between the sleeve and the liquid taking telescopic tube 2-46, i.e. the movement in the Z-axis direction;

the marks 2-45 are sleeves, the liquid taking telescopic pipes 2-46 are arranged on the sleeves in a sliding manner and driven by a motor III 2-44 to move downwards, so that the liquid transferring suction heads loaded on the liquid taking telescopic pipes 2-46 can be unloaded to a garbage can area, and the outer diameter of the device is larger than or equal to the diameter of the upper end shown in the figure 3;

the mark 2-47 is a piston type air pump, under the control of the microcomputer chip, a certain amount of air is sucked or exhausted, so that the sample/nucleic acid placed in the EP tube placing area 4 is sucked and added into the PCR reaction tube 5, and the device is tightly connected with the lower end opening of the liquid taking telescopic tube through connecting tubes 2-48;

the marks 2-48 are connecting pipes, the device can tightly connect the piston type air pump with the front end opening of the liquid taking telescopic pipe, the liquid moving suction head is loaded on the connecting pipes, and when the air tightness detection needle shown in figure 3 is used, an airtight environment which keeps a certain negative pressure is formed.

The device needs a microcomputer chip control module and needs to complete the following work:

finishing the display and control instruction of the area shown by the touch screen in FIG. 1;

controlling the trend and the positioning of the figure 4 in the directions of x, y and z axes;

automatically calculating the liquid taking amount and the liquid dividing amount according to the operation instruction, and controlling the graph 4 to finish the program;

the utility model discloses a concrete working process:

(1) placing the reagents/nucleic acids to be dispensed into the wells in the area of FIG. 1 (e.g., at the 2C position);

(2) placing the PCR reaction tube (eight tubes or 96-well plate) in position No. 5 in FIG. 1;

(3) operating in the control area of the touch screen 7 of FIG. 1, selecting the position (e.g. 2C) of the EP tube placement area in FIG. 1 for storing the nucleic acid, and further selecting which well (e.g. B3.B4. B5) of the PCR reaction tube in FIG. 1 needs to be dispensed, and further selecting the sample addition amount (e.g. 3. mu.L) of each reaction well;

(4) the liquid taking device shown in fig. 4 receives a control command, firstly, the liquid taking device moves to the position of the first rail in fig. 1, the second motor 2-43 in fig. 4 drives the liquid taking telescopic pipe to extend downwards to load the liquid transferring suction head, and then the liquid taking telescopic pipe returns to the original position;

(5) FIG. 4 shows the first motor 2-42 driving the liquid extractor 2-4 to move along the third rail to the position 2C in the connection region of FIG. 1;

(6) FIG. 4, the second motor 2-43 drives the liquid taking telescopic tube to extend downwards again, so that the liquid transfer suction head is immersed below the liquid level, the piston air pump 2-47 is started to accurately suck 10 mu L of liquid in the hole 2C, the liquid taking telescopic tube returns to the original position after the liquid taking telescopic tube is completed, and the piston air pump maintains the existing position unchanged;

(7) the driving motors 2-6 drive the whole upright post bracket to move to the PCR reaction tube area in the figure 1 along the first rail and accurately move to three positions B3, B3 and B5 in sequence;

(8) the second motor 2-43 drives the liquid taking telescopic pipe to extend downwards again, so that the liquid transfer suction head is immersed below the liquid level, the piston air pump 2-47 is started, 3 mu L of liquid is accurately pushed out to three PCR reaction holes B3.B5 respectively, the liquid transfer suction head returns after the completion, and meanwhile, the piston air pump 2-47 returns;

(9) FIG. 2 shows that the driving motor drives the upright post bracket to integrally move to the position of 6 in the garbage can area, the motor III 2-44 drives the sleeve 2-45 to move downwards, and the liquid-transferring suction head used at this time is unloaded into the garbage can to complete the liquid-adding and separating process at this time;

(10) multiple samples are analogized in turn.

The technical features that the utility model has not been described can be realized through or adopt prior art, and no longer give unnecessary details here, and of course, the above-mentioned explanation is not right the utility model discloses a restriction, the utility model discloses also not only be limited to the above-mentioned example, ordinary skilled person in this technical field is in the utility model discloses a change, modification, interpolation or replacement made in the essential scope also should belong to the utility model discloses a protection scope.

Claims (5)

1. The utility model provides an automatic application of sample platform for biological assay, its characterized in that, include that EP manages and places the district, be close to the district is placed to PCR pipe that EP managed and placed the district, move liquid suction head box and place position, touch-sensitive screen, garbage bin region, gas tightness detection needle and place position and application of sample mechanism, gas tightness detection needle is placed the position and is set up move between liquid suction head box and place the position and the touch-sensitive screen, application of sample mechanism connects on track one, track one sets up and is being moved between liquid suction head box and place the position and EP manages and place the district, application of sample mechanism is connected with control chip.

2. The automatic sample application platform according to claim 1, wherein the sample application mechanism comprises a vertical column support disposed on the first rail, a horizontal shaft horizontally disposed at a top end of the vertical column support, and a liquid taking device, the vertical column support is slidably disposed on the first rail by a driving motor, a second rail is disposed on the horizontal shaft of the vertical column support along a length direction of the horizontal shaft, and the liquid taking device is slidably disposed on the second rail.

3. The automatic sample adding platform for the biological test according to claim 2, wherein the liquid taking device comprises a connecting block, a liquid taking telescopic pipe connected with the connecting block, a first motor and a second motor which are arranged at two ends of the connecting block, a third motor attached to the liquid taking telescopic pipe, a sleeve, an air pump and a connecting pipe connected with the air pump, the connecting block is slidably arranged on the transverse shaft through the first motor, the liquid taking telescopic pipe is connected with the connecting block through the second motor, the liquid taking telescopic pipe is slidably arranged in the sleeve through the third motor, and the air pump is arranged on the liquid taking telescopic pipe.

4. The automatic sample application platform for biological assay as claimed in claim 3, wherein the bottom of the air-tight detection needle is hermetically designed and has an external shape conforming to that of a common pipetting tip.

5. The automatic sample application platform for bioassay according to claim 1, wherein the EP tube placing section and the PCR tube placing section are provided with a refrigerating device.

Images