CN219546981U

CN219546981U - Novel automatic liquid ware that trades of cell

Info

Publication number: CN219546981U
Application number: CN202223450977.2U
Authority: CN
Inventors: 刘帅; 潘佳欣; 王薇; 魏本杰; 栾国庆
Original assignee: HEILONGJIANG HENGSHENG STEM CELL ENGINEERING CO LTD; Yinfeng Biological Group Ltd
Current assignee: HEILONGJIANG HENGSHENG STEM CELL ENGINEERING CO LTD; Yinfeng Biological Group Ltd
Priority date: 2022-12-23
Filing date: 2022-12-23
Publication date: 2023-08-18
Anticipated expiration: 2032-12-23

Abstract

The utility model discloses a novel automatic cell liquid changer, wherein an automatic liquid changer body is provided with a needle end, a control end and a tank body end, the needle end is arranged at the front part of the automatic liquid changer body, the needle end is provided with a sucking needle and an injecting needle, the tank body end is arranged at the rear part of the automatic liquid changer body, the tank body end comprises a waste liquid bottle and a culture liquid bottle, the sucking needle and the injecting needle are respectively connected with a liquid pipe, the other end of the liquid pipe is connected with the waste liquid bottle and the culture liquid bottle, the control end comprises a control handle, a motor and a rotating shaft, and the control handle is provided with a power switch, a speed shift-up and shift-down, a sucking control button and an injecting control button. The suction needle and the injection needle can be pulled down to the use height and locked at the use height when the suction needle and the injection needle are needed to be used, and can be lifted to the hanging position when the suction needle and the injection needle are not used, so that cross contamination can be effectively placed; the liquid output can be controlled by the microcomputer controller, and deviation occurs in placement, so that the result is more accurate.

Description

Novel automatic liquid ware that trades of cell

Technical Field

The utility model relates to the field of laboratory technical equipment, in particular to a novel automatic cell liquid exchanger.

Background

The electric sucking aid is the most commonly used pipetting and separating equipment in the field of cell biology at present, and is a relatively light AC/DC dual-purpose pipetting controller capable of being charged repeatedly. The device is mainly used in a laboratory for transferring reagents, can be arbitrarily inserted into a glass pipette, can suck liquid with a preset volume by manually pressing down a switch, and replaces the prior process of sucking the solution by using an ear suction ball in the laboratory. However, in the cell factory, 40 bottles of T175 culture bottles are often needed for one passage, so the electric sucking assisting device at least needs to repeat the actions of sucking and beating out 40 times, the workload of an operator is very large, and the novel automatic cell liquid changing device is designed to solve the problem.

The prior art has the defects that: firstly, the existing auxiliary aspirator needs to be matched with a pipette for use; secondly, in a large-scale cell factory, the number of culture bottles for changing liquid each time is tens of bottles, so that the sucking and beating actions are repeated for at least tens of times; third, aspiration and infusion volumes need to be visually inspected through the scale on the pipette, and there may be deviations due to the fact that the line of sight is not on the same horizontal plane, etc.

Disclosure of Invention

The utility model aims to solve the technical problems that firstly, the existing auxiliary aspirator needs to be matched with a pipette for use; secondly, in a large-scale cell factory, the number of culture bottles for changing liquid each time is tens of bottles, so that the sucking and beating actions are repeated for at least tens of times; third, aspiration and infusion volumes need to be visually inspected through the scale on the pipette, and there may be deviations due to the fact that the line of sight is not on the same horizontal plane, etc.

In order to solve the technical problems, the utility model adopts the following technical means:

a novel automatic cell liquid exchanger comprises an automatic liquid exchanger body; the automatic liquid changer body be equipped with needle end, control end and jar body end, the needle end set up in the front portion of automatic liquid changer body, the needle end is equipped with and absorbs needle and injection needle, jar body end set up the rear portion at automatic liquid changer body, jar body end include waste liquid bottle and culture solution bottle, absorption needle and injection needle connect the liquid pipe respectively, waste liquid bottle and culture solution bottle are connected to the other end of liquid pipe, the control end include control handle, motor and axis of rotation, the control handle on be equipped with switch, speed and add shelves, absorb control button and injection control button. The technology upgrades and reforms the original auxiliary aspirator, solves the problem of long-time use convenience and reduces fatigue.

Preferably, the utility model further adopts the technical scheme that:

the sucking needle and the injection needle are arranged at the opening part of the front part of the needle head end, one side of the needle head end is provided with a sucking needle and an injection needle control button, the sucking needle and the injection needle control button can slide on the slide way, and the sucking needle and the injection needle control button move up and down in the slide way.

The power switch controls the automatic liquid changer body to be opened and closed through the data line and the power line, the speed of the motor is controlled by the speed up-down gear, and the suction control button and the injection control button respectively control the start and the close of the suction needle and the injection needle.

The motor drives the rotating shaft to rotate in a starting state, the shaft rotation comprises an A shaft, a B shaft and a C shaft, and the liquid pipe is extruded through the shaft rotation, so that positive pressure or negative pressure is formed in the liquid pipe.

The liquid pipe include first liquid pipe and second liquid pipe, first liquid pipe connection waste liquid bottle and cell culture household utensils, the liquid in the cell culture household utensils flows into the waste liquid bottle along first liquid pipe, second liquid pipe connection blake bottle and cell culture household utensils, the liquid in the blake bottle is through the injection of second liquid pipe in the cell culture household utensils.

The rotating shafts comprise a first group of rotating shafts and a second group of rotating shafts, the first group of rotating shafts are arranged on the first liquid pipe and used for controlling the communication between the waste liquid bottle and the cell culture vessel, and the second group of rotating shafts are arranged on the second liquid pipe and used for controlling the communication between the culture bottle and the cell culture vessel.

One side of the control handle is provided with a supporting frame which can be opened or closed.

The control end is provided with a micro-controller, and the micro-controller comprises a built-in chip and an LED display screen.

According to the novel automatic cell liquid changer, when a large number of cells change liquid, the liquid in the multi-bottle cell culture vessel can be directly sucked out to a waste liquid bottle by utilizing the sucking function, so that the step of transferring a pipette is omitted; the liquid can also be directly drawn from fresh culture flasks for injection when the culture medium is added to the cell culture vessel. Mainly solves the problems of long-time use convenience and fatigue reduction; the suction needle and the injection needle can be pulled down to the use height and locked at the use height when the suction needle and the injection needle are needed to be used, and can be lifted to the hanging position when the suction needle and the injection needle are not used, so that cross contamination can be effectively placed; the liquid output can be controlled by the microcomputer controller, and deviation occurs in placement, so that the result is more accurate.

Drawings

Fig. 1 is a front view of an embodiment of the present utility model.

Fig. 2 is an internal structural view of an embodiment of the present utility model.

Fig. 3 is an enlarged schematic view of the rotating shaft and the liquid pipe according to the present utility model.

Reference numerals illustrate: 01. a micro controller; 02. aspiration needle control buttons; 03. a slideway; 04. a support frame; 05. a power switch; 06. speed upshifting; 07. sucking a control button; 08. an injection control button; 09. a motor; 10. a rotating shaft; 11. a suction needle; 13. an injection needle; 13, a waste liquid bottle; 14. culture solution bottle.

Detailed Description

The utility model will be further illustrated with reference to the following examples.

Referring to fig. 1 and 2, in the novel automatic cell liquid exchanger of the present utility model, when the power switch 05 is turned on and liquid needs to be sucked out, the sucking needle control button 02 and 02 slide from top to bottom on the slideway 03 to fix the bottom, so as to drive the sucking needle 11 to slide out of the liquid exchanger; the suction needle is inserted into the cell culture vessel, the suction control button 07 is clicked, the motor 09 in the liquid changer drives the rotating shaft 10 to rotate, the liquid pipe is extruded, negative pressure is formed in the liquid pipe, liquid in the cell culture vessel is pumped out, and the liquid flows into the waste liquid bottle 13 along the liquid pipe. Also, if it is desired to inject culture medium into the cell culture vessel, the liquid in the extraction flask 14 is injected into the cell culture vessel.

Referring to fig. 1 and 2, the novel automatic cell liquid exchanger of the present utility model is characterized in that a motor drives a rotating shaft to rotate to squeeze a liquid tube, and the flowing direction of liquid is controlled by the rotating direction; the sucking needle channel can extend out of the liquid exchanger or retract back to the liquid exchanger through the control switch; the injection needle is arranged on the same; the sucking needle is connected through a liquid pipe, and liquid in the sucking cell culture vessel flows into the waste liquid bottle along the liquid pipe; the injection needle is connected through a liquid pipe, and liquid in the culture liquid bottle is sucked and injected into a culture vessel, such as a T175 culture bottle; for the convenience of operators, the automatic cell liquid exchanger is provided with a handle, a bracket is arranged beside an arm, and the bracket can be folded; and a microcomputer controller is provided to display the remaining power, set the liquid output, etc.

Since the above description is only specific embodiments of the present utility model, the protection of the present utility model is not limited thereto, and any equivalent changes or substitutions of technical features of the present utility model will be apparent to those skilled in the art, and are included in the scope of the present utility model.

Claims

1. A novel automatic cell liquid exchanger comprises an automatic liquid exchanger body; the method is characterized in that: the automatic liquid changer body be equipped with needle end, control end and jar body end, the needle end set up in the front portion of automatic liquid changer body, the needle end is equipped with and absorbs needle and injection needle, jar body end set up the rear portion at automatic liquid changer body, jar body end include waste liquid bottle and culture solution bottle, absorption needle and injection needle connect the liquid pipe respectively, waste liquid bottle and culture solution bottle are connected to the other end of liquid pipe, the control end include control handle, motor and axis of rotation, the control handle on be equipped with switch, speed and add shelves, absorb control button and injection control button.

2. The novel automatic cell liquid exchanger according to claim 1, wherein: the sucking needle and the injection needle are arranged at the opening part of the front part of the needle head end, one side of the needle head end is provided with a sucking needle and an injection needle control button, the sucking needle and the injection needle control button can slide on the slide way, and the sucking needle and the injection needle control button move up and down in the slide way.

3. The novel automatic cell liquid exchanger according to claim 1, wherein: the power switch controls the automatic liquid changer body to be opened and closed through the data line and the power line, the speed of the motor is controlled by the speed up-down gear, and the suction control button and the injection control button respectively control the start and the close of the suction needle and the injection needle.

4. The novel automatic cell liquid exchanger according to claim 1, wherein: the motor drives the rotating shaft to rotate in a starting state, the shaft rotation comprises an A shaft, a B shaft and a C shaft, and the liquid pipe is extruded through the shaft rotation, so that positive pressure or negative pressure is formed in the liquid pipe.

5. The novel automatic cell liquid exchanger according to claim 1, wherein: the liquid pipe include first liquid pipe and second liquid pipe, first liquid pipe connection waste liquid bottle and cell culture household utensils, the liquid in the cell culture household utensils flows into the waste liquid bottle along first liquid pipe, second liquid pipe connection blake bottle and cell culture household utensils, the liquid in the blake bottle is through the injection of second liquid pipe in the cell culture household utensils.

6. The novel automatic cell liquid exchanger according to claim 1, wherein: the rotating shafts comprise a first group of rotating shafts and a second group of rotating shafts, the first group of rotating shafts are arranged on the first liquid pipe and used for controlling the communication between the waste liquid bottle and the cell culture vessel, and the second group of rotating shafts are arranged on the second liquid pipe and used for controlling the communication between the culture bottle and the cell culture vessel.

7. The novel automatic cell liquid exchanger according to claim 1, wherein: one side of the control handle is provided with a supporting frame which can be opened or closed.

8. The novel automatic cell liquid exchanger according to claim 1, wherein: the control end is provided with a micro-controller, and the micro-controller comprises a built-in chip and an LED display screen.