CN116535494B - 一种重组类人源ⅲ型胶原蛋白及其应用 - Google Patents
一种重组类人源ⅲ型胶原蛋白及其应用 Download PDFInfo
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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Abstract
Description
胶原蛋白COL3A1-QS-1 | |
氨基酸序列 | GTSGYQGKDGESGERGHRGRNGEKGETGENGENGERGNDGSDGSNGQRGKNGERGKDGERGEKGERGRDGSDGSQGESGNDGKNGERGKNGETGDKGDTGENGERGERGDKGKDGDKGERGETGQNGERGEKGERGSNGKDGNTGEKGRDGRDGDRGENGKSGDRGESGSRGDKGETGERGHRGQQGKDGTSGNRGERGSE(SEQ ID NO:1) |
核酸序列 | GGTACTTCTGGTTATCAAGGTAAAGATGGTGAATCTGGTGAAAGAGGTCATAGAGGTAGAAATGGTGAAAAAGGTGAAACTGGTGAAAATGGTGAAAACGGTGAAAGAGGAAACGATGGTTCTGATGGTTCTAATGGTCAAAGAGGTAAAAACGGTGAAAGAGGTAAAGATGGAGAAAGAGGTGAAAAAGGAGAAAGAGGAAGAGATGGTTCTGACGGTTCTCAAGGTGAATCTGGAAACGATGGAAAAAACGGTGAGAGAGGTAAAAATGGTGAAACTGGAGATAAGGGTGACACTGGTGAAAACGGAGAAAGAGGTGAGAGAGGAGATAAAGGTAAAGATGGTGACAAAGGTGAAAGAGGTGAAACTGGTCAAAATGGTGAAAGAGGTGAAAAGGGTGAAAGAGGTTCTAACGGTAAAGATGGTAACACTGGTGAAAAAGGTAGAGATGGTAGAGATGGAGATAGAGGTGAAAACGGTAAATCTGGTGACAGAGGTGAATCTGGTTCTAGAGGTGACAAAGGAGAAACTGGTGAGAGAGGTCATAGAGGACAACAAGGTAAAGACGGTACTTCTGGAAACAGAGGTGAAAGAGGTTCCGAAtaa(SEQ ID NO:2) |
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CN114540363A (zh) * | 2022-02-17 | 2022-05-27 | 江南大学 | 一种类人胶原蛋白重组毕赤酵母工程菌的构建和蛋白快速纯化方法 |
CN114920826A (zh) * | 2022-06-15 | 2022-08-19 | 江南大学 | Ⅲ型类人胶原蛋白及其编码基因、表达载体和重组酵母菌 |
CN115558612A (zh) * | 2021-07-01 | 2023-01-03 | 华北制药集团新药研究开发有限责任公司 | 重组全长ⅲ型人源化胶原蛋白酵母工程菌株及构建方法 |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112724242A (zh) * | 2020-12-30 | 2021-04-30 | 西安德诺海思医疗科技有限公司 | 利用毕赤酵母生产重组类人胶原蛋白和宿主细胞蛋白的方法 |
CN115558612A (zh) * | 2021-07-01 | 2023-01-03 | 华北制药集团新药研究开发有限责任公司 | 重组全长ⅲ型人源化胶原蛋白酵母工程菌株及构建方法 |
CN113621053A (zh) * | 2021-08-30 | 2021-11-09 | 江苏亨瑞生物医药科技有限公司 | 一种重组人源胶原蛋白及其制备方法和应用 |
CN114540363A (zh) * | 2022-02-17 | 2022-05-27 | 江南大学 | 一种类人胶原蛋白重组毕赤酵母工程菌的构建和蛋白快速纯化方法 |
CN114920826A (zh) * | 2022-06-15 | 2022-08-19 | 江南大学 | Ⅲ型类人胶原蛋白及其编码基因、表达载体和重组酵母菌 |
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