CN116515757A - 基于使用表达增强性基因座来制备抗体的组合物和方法 - Google Patents

基于使用表达增强性基因座来制备抗体的组合物和方法 Download PDF

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Publication number
CN116515757A
CN116515757A CN202211606177.7A CN202211606177A CN116515757A CN 116515757 A CN116515757 A CN 116515757A CN 202211606177 A CN202211606177 A CN 202211606177A CN 116515757 A CN116515757 A CN 116515757A
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rrs
vector
hcf
nucleotide sequence
lcf
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Chinese (zh)
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R·巴布
D·布拉科夫
陈刚
J·P·梵迪
Y·赵
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN202211606177.7A 2016-04-20 2017-04-20 基于使用表达增强性基因座来制备抗体的组合物和方法 Pending CN116515757A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662325400P 2016-04-20 2016-04-20
US62/325,400 2016-04-20
PCT/US2017/028555 WO2017184832A1 (en) 2016-04-20 2017-04-20 Compositions and methods for making antibodies based on use of expression-enhancing loci
CN201780024557.0A CN109195986B (zh) 2016-04-20 2017-04-20 基于使用表达增强性基因座来制备抗体的组合物和方法

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US (2) US11512144B2 (cg-RX-API-DMAC7.html)
EP (1) EP3445781A1 (cg-RX-API-DMAC7.html)
JP (4) JP7096770B2 (cg-RX-API-DMAC7.html)
KR (3) KR102547738B1 (cg-RX-API-DMAC7.html)
CN (2) CN116515757A (cg-RX-API-DMAC7.html)
AR (1) AR109451A1 (cg-RX-API-DMAC7.html)
AU (2) AU2017253241B2 (cg-RX-API-DMAC7.html)
BR (1) BR112018071283A2 (cg-RX-API-DMAC7.html)
CA (1) CA3015389A1 (cg-RX-API-DMAC7.html)
EA (1) EA201892010A1 (cg-RX-API-DMAC7.html)
IL (2) IL314779A (cg-RX-API-DMAC7.html)
MX (2) MX2018012868A (cg-RX-API-DMAC7.html)
SG (2) SG11201807885PA (cg-RX-API-DMAC7.html)
TW (1) TWI827531B (cg-RX-API-DMAC7.html)
WO (1) WO2017184832A1 (cg-RX-API-DMAC7.html)

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AR127674A1 (es) 2021-10-18 2024-02-21 Regeneron Pharma Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios
JP2024537896A (ja) 2021-10-18 2024-10-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞
CN118159660A (zh) 2021-10-18 2024-06-07 瑞泽恩制药公司 多核苷酸的受控转录
CN119731330A (zh) * 2021-12-22 2025-03-28 基因泰克公司 多载体重组酶介导的盒式交换
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CN121794393A (zh) 2023-09-07 2026-04-03 里珍纳龙药品有限公司 经共价表面修饰的腺相关病毒的生产和纯化
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus
WO2025115876A1 (ja) 2023-11-29 2025-06-05 富士フイルム株式会社 細胞の作出方法、細胞及びタンパク質の製造方法
KR20260103112A (ko) 2023-11-29 2026-07-02 후지필름 가부시키가이샤 탐색 방법, 세포의 작출 방법, 세포의 선별 방법, 세포 및 세포 생산물의 제조 방법
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production

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CN109195986A (zh) 2019-01-11
MX2018012868A (es) 2019-03-11
TWI827531B (zh) 2024-01-01
IL262266A (en) 2018-11-29
TW201803986A (zh) 2018-02-01
US12528883B2 (en) 2026-01-20
IL262266B1 (en) 2024-09-01
EA201892010A1 (ru) 2019-03-29
IL262266B2 (en) 2025-01-01
JP7096770B2 (ja) 2022-07-06
KR20260011194A (ko) 2026-01-22
SG10202010155YA (en) 2020-11-27
EP3445781A1 (en) 2019-02-27
IL314779A (en) 2024-10-01
JP7781128B2 (ja) 2025-12-05
CA3015389A1 (en) 2017-10-26
SG11201807885PA (en) 2018-10-30
WO2017184832A1 (en) 2017-10-26
KR102547738B1 (ko) 2023-06-26
JP2025188169A (ja) 2025-12-25
KR20180134893A (ko) 2018-12-19
AR109451A1 (es) 2018-12-12
US11512144B2 (en) 2022-11-29
TW202415767A (zh) 2024-04-16
JP2021164479A (ja) 2021-10-14
BR112018071283A2 (pt) 2019-02-12
JP2024023449A (ja) 2024-02-21
JP2019515666A (ja) 2019-06-13
KR102906454B1 (ko) 2026-01-02
AU2017253241A1 (en) 2018-09-20
CN109195986B (zh) 2023-01-03
KR20230098361A (ko) 2023-07-03
MX2025003852A (es) 2025-05-02
US20230130799A1 (en) 2023-04-27
US20190233544A1 (en) 2019-08-01
AU2024220124A1 (en) 2024-10-17
AU2017253241B2 (en) 2024-07-04

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