CN116473900A - Natural alopecia preventing composition and preparation method and application thereof - Google Patents
Natural alopecia preventing composition and preparation method and application thereof Download PDFInfo
- Publication number
- CN116473900A CN116473900A CN202310655302.1A CN202310655302A CN116473900A CN 116473900 A CN116473900 A CN 116473900A CN 202310655302 A CN202310655302 A CN 202310655302A CN 116473900 A CN116473900 A CN 116473900A
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- extract
- hair loss
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Classifications
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Landscapes
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- Organic Chemistry (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a natural anti-hair loss composition and a preparation method thereof, wherein the active ingredients of the composition comprise: nine natural extract components of polygonum multiflorum, angelica sinensis, glossy privet fruit, rhizoma drynariae, ginger, biota orientalis, saussurea involucrata, chilli and citrus are extracted, and the nine natural extract components are prepared by respectively adopting different physical or chemical extraction and purification methods for polygonum multiflorum, angelica sinensis, glossy privet fruit, rhizoma drynariae, ginger, biota orientalis, saussurea involucrata, chilli and citrus; the natural anti-hair loss composition can be added into conventional scalp washing and caring components or products, and has the effects of inhibiting the proliferation of microorganisms, repairing damaged hair follicles and reducing hair loss.
Description
Technical Field
The invention belongs to the technical field of washing and caring daily necessities, and particularly relates to a natural anti-hair loss composition, and a preparation method and application thereof.
Background
With the current living environment influence including the rising of living pressure, the problems of excessive scalp grease secretion, dandruff, scalp sensitivity and the like are more and more common, so that the efficacy requirement of consumers on the hair care products is gradually increased, wherein the imbalance of scalp grease secretion is realized, the functions of the hair care products are continuously extended and gradually refined in terms of product functions, the functions of initial hair cleaning are gradually extended into the functions of oil control, dandruff removal, nursing, moistening and the like, and the functions of hair blacking, hair loss prevention, hair growth and the like are further extended.
Aiming at scalp and hair washing and caring products with repairing effect, the components of the washing and caring products on the market at present mainly adopt chemical synthetic substances, and various side effects such as dry and yellow hair, broken hair, hair loss and the like are shown after long-term use by consumers. Moreover, with the popularity of environmental protection, low carbon, green and natural trends in recent years, plant anti-dandruff and antipruritic shampoo is a trend in the future, but the effect is not obvious although a plurality of herbaceous plants are used in the current plant shampoo, for example, chinese patent CN106619385A discloses a traditional Chinese medicine anti-hair loss shampoo and a preparation method thereof, the shampoo adopts arborvitae tops, medlar, old ginger, arborvitae seeds, rhizoma drynariae, red sage roots, rhizoma polygonati, clove, glossy privet fruits, safflower, radix rehmanniae, red paeony roots, peach kernels, fleece-flower roots, white mulberry barks and fructus psoraleae, and the traditional Chinese medicinal materials adopted by the shampoo are added by the conventional crushing means, and a great amount of macromolecules or high polymer components such as proteins are difficult to obtain better absorption and obstruct the permeation effect of active substances, so the anti-hair loss effect of the technical scheme is not ideal; for example, chinese patent CN105362954a discloses a traditional Chinese medicine for treating baldness and a preparation method thereof, wherein the traditional Chinese medicine comprises lotus plumule, cortex acanthopanacis, eclipta, sesame flower, cockscomb, achyranthes root, dried rehmannia root, fructus aurantii, semen cuscutae, cortex lycii radicis, locust bean, rhizoma polygonati, fructus cnidii, dried orange peel, radix curcumae, radix philippine flemingiae, angelica sinensis, fructus lycii, glossy privet fruit, rehmannia root, radix astragali, polygonum multiflorum, cacumen biotae, eclipta, ligusticum wallichii and spatholobus stem, and the traditional Chinese medicine is extracted and not purified, and still contains a large amount of macromolecular substances, and the macromolecular substances such as protein and the like have no effect on the cleaning effect of hair follicles. Based on the anti-drop product which has been developed in the prior art and takes Chinese medicinal materials as main active ingredients, the washing and caring product which is prepared from the high-efficiency anti-drop Chinese medicinal extract component and has no side effect has more significance for the utilization rate of the active ingredients of the Chinese medicinal materials and alopecia crowd.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a natural anti-hair loss composition, a preparation method and application thereof, and the natural anti-hair loss composition prepared by the invention has abundant active ingredients of natural products, can promote the cleaning and repair of scalp hair follicles, increases the permeability of the active ingredients and improves the anti-hair loss effect.
In a first aspect, the present invention provides a natural anti-hair loss composition comprising polygonum multiflorum extract, angelica extract, glossy privet fruit extract, drynaria extract, ginger extract, biota orientalis extract, saussurea involucrata extract, capsicum extract and citrus extract.
Further, the natural anti-hair loss composition comprises, by weight, 1-5 parts of polygonum multiflorum extract, 0.1-3 parts of angelica sinensis extract, 0.5-5 parts of glossy privet fruit extract, 0.1-2 parts of drynaria rhizome extract, 0.5-5 parts of ginger extract, 0.1-3 parts of biota orientalis extract, 0.1-3 parts of saussurea involucrata extract, 0.1-2 parts of chilli extract and 0.1-2 parts of citrus extract.
In a second aspect, the present invention provides a method for preparing nine natural extract components in the natural anti-hair loss composition, which comprises the following specific preparation methods:
(1) The preparation method of the polygonum multiflorum extract comprises the following steps: cleaning tuber fleeceflower root, drying, crushing, soaking in solvent, reflux extracting, filtering and concentrating to obtain extractum, stepped extracting with solvent, filtering and concentrating to obtain coarse fraction, column chromatographic treatment of the coarse fraction, and concentrating the eluted fraction to obtain tuber fleeceflower root extract.
(2) The preparation method of the angelica sinensis extract comprises the following steps: soaking radix Angelicae sinensis powder in solvent, reflux extracting, filtering, concentrating to obtain extract, subjecting the extract to fractional extraction with solvent, filtering, concentrating to obtain crude fraction, subjecting the crude fraction to column chromatography, and concentrating the eluted fraction to obtain radix Angelicae sinensis extract.
(3) The preparation method of the glossy privet fruit extract comprises the following steps: pulverizing fructus Ligustri Lucidi, soaking in solvent, reflux extracting, filtering, concentrating to obtain extract, subjecting the extract to fractional extraction with solvent, filtering, concentrating to obtain crude fraction, subjecting the crude fraction to column chromatography, and concentrating the eluted fraction to obtain fructus Ligustri Lucidi extract.
(4) The preparation method of the drynaria extract comprises the following steps: soaking rhizoma Drynariae powder in solvent, reflux extracting, filtering, concentrating to obtain extract, subjecting the extract to fractional extraction with solvent, filtering, concentrating to obtain crude fraction, subjecting the crude fraction to column chromatography, and concentrating the eluted fraction to obtain rhizoma Drynariae extract.
(5) The preparation method of the ginger extract comprises the following steps: cleaning rhizoma Zingiberis recens, mashing, soaking in water, ultrasonic treating, filtering, soaking the residue in water of the same quality, ultrasonic treating, filtering, repeating the same steps for three times, mixing the three filtrates, and concentrating to obtain rhizoma Zingiberis recens extract.
(6) The preparation method of the biota orientalis leaf extract comprises the following steps: cleaning folium Platycladi, drying, pulverizing, soaking in hot water, ultrasonic treating, filtering, deproteinizing the filtrate, dialyzing, and concentrating to obtain folium Platycladi extract.
(7) The preparation method of the lotus seed extract comprises the following steps: cleaning herba Saussureae Involueratae, mashing, soaking in solvent, ultrasonic treating, filtering, reflux extracting, filtering, mixing filtrates, concentrating to obtain crude extract, respectively extracting with solvent, filtering, mixing filtrates, and concentrating to obtain herba Saussureae Involueratae extract.
(8) The preparation method of the chilli extract comprises the following steps: cleaning capsicum, drying, pulverizing, soaking in solvent, ultrasonic treating, filtering, repeating the steps for 3-5 times, mixing filtrates, and concentrating to obtain capsicum extract.
(9) The preparation method of the citrus extract comprises the following steps: cutting citrus peel, soaking in distilled water, heat extracting, filtering, and drying to obtain citrus extract.
The solvent used in the preparation process of the nine natural extract components is one or more selected from methanol, ethanol, petroleum ether, dichlorohexane, chloroform, ethyl acetate, n-butanol, isobutanol and n-hexane.
In a third aspect, the present invention also provides the use of the hair care composition in a scalp, hair or hair follicle repair care product.
Further, the scalp, hair or hair follicle repair care product is in any form of liquid, paste, emulsion, foam or spray, but the product is not limited to the form.
Compared with the prior art, the natural anti-hair loss composition prepared by the invention adopts nine natural extract components, the compatibility among the natural extract components is good, different extraction and purification means are adopted for different natural traditional Chinese medicinal material components, the extraction of active components is realized, and the finally formed composition has rich natural product active components, so that the cleaning and repairing of scalp hair follicles can be promoted, the permeability of the active components is increased, and the anti-hair loss effect and effect are improved.
Detailed Description
The experimental methods of the present invention, in which specific conditions are not specified in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer. The various chemicals commonly used in the examples are commercially available.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The terms "comprising" and "having" and any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, apparatus, article, or device that comprises a list of steps is not limited to the elements or modules listed but may alternatively include additional steps not listed or inherent to such process, method, article, or device.
The present invention will be further described in detail with reference to the following embodiments, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the description is only illustrative and is not intended to limit the scope of the invention. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the present invention.
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.
EXAMPLE 1 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 1 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 2 parts of polygonum multiflorum extract, 3 parts of angelica extract, 0.5 part of glossy privet fruit extract, 2 parts of drynaria extract, 3 parts of ginger extract, 0.4 part of biota orientalis extract, 2.8 parts of saussurea involucrata extract, 0.5 part of chilli extract and 1.1 part of citrus extract.
The preparation method of the polygonum multiflorum extract comprises the following steps: washing, drying and crushing tuber fleeceflower root, taking methanol (70 wt%) of tuber fleeceflower root powder with 12 times of mass, soaking for 6h, then carrying out reflux extraction for 2h, concentrating the filtered filtrate to obtain extract, sequentially extracting the extract with petroleum ether (3 times of the mass of the extract) for 3 times, extracting the extract with ethyl acetate (3 times of the mass of the extract) for 3 times, extracting the extract with methanol (2 times of the mass of the extract) for 3 times, respectively carrying out filtration and concentration to obtain petroleum ether section crude fraction, ethyl acetate section crude fraction and methanol section crude fraction, carrying out column chromatography on the ethyl acetate section crude fraction, loading the column with ODS reverse phase silica gel packing, eluting with a methanol-water eluting system, eluting gradient of 30:70, 40:60, 50:50, 60:40, 70:30, 100:0, each gradient eluting for 2 column volumes, sequentially obtaining A-F6 fragments, collecting fraction A-D, merging and concentrating, further carrying out Sephax LH-20 column chromatography, eluting with methanol with a flow rate of 1-2 drops/second, collecting a tube, collecting 2ml of eluate with a thin layer chromatography (d=170 cm), and carrying out TLC analysis on the concentrated extract, obtaining the tuber fleeceflower root extract after the Thin Layer Chromatography (TLC).
The preparation method of the angelica sinensis extract comprises the following steps: soaking angelica sinensis powder in methanol (70 wt%) of 12 times of the mass of the angelica sinensis powder for 24 hours, filtering, adding 2 times of the mass of methanol (70 wt%) into filter residues for reflux extraction for 2 hours, filtering, combining the two filtrates, concentrating to obtain an extract, extracting the extract by petroleum ether (3 times of the mass of the extract) 3 times, extracting the dichloromethane (3 times of the mass of the extract) 3 times, extracting the ethyl acetate (3 times of the mass of the extract) 3 times, extracting the methanol (2 times of the mass of the extract) 3 times, filtering and concentrating respectively to obtain a petroleum ether section crude fraction, a dichloromethane section crude fraction, an ethyl acetate section crude fraction and a methanol section crude fraction, and carrying out column chromatography on the petroleum ether section crude fraction, the dichloromethane section crude fraction and the ethyl acetate section crude fraction respectively, wherein the steps are as follows:
petroleum ether section crude fraction: eluting 2 column volumes by adopting a normal-phase silica gel column, eluting with a methylene dichloride-methanol (volume ratio of 1:1) elution system, eluting at a flow rate of 5-6 drops/second, collecting 5ml of fractions with 2 column volumes (d=4cm, h=150cm), performing TLC thin-layer chromatography on the fractions, combining the 17 th-25 th tubes, concentrating to obtain a fraction A, eluting the fraction A by using an ODS reverse-phase silica gel column, eluting with a methanol-water elution system, eluting with a gradient of 40:60, 50:50, 60:40, 70:30 and 100:0, eluting with 2 column volumes of each gradient, sequentially obtaining A-E5 fraction segments, collecting fraction A-D, combining and concentrating, performing Sephadex LH-20 gel column chromatography on the fractions, eluting with methanol at a flow rate of 1-2 drops/second, collecting 2ml of fractions with 2 column volumes (d=3cm, h=170cm), performing TLC thin-layer chromatography on the fractions, combining the fractions, and concentrating to obtain fraction 1.
Methylene chloride section crude fraction: the method comprises the steps of filling a column with ODS reverse phase silica gel packing, eluting with a methanol-water eluting system, eluting with methanol and water in a volume ratio of 35:65, 50:50, 70:30 and 100:0, eluting 2 column volumes (d=4cm and h=150cm) in each gradient, sequentially obtaining A-D4 fraction segments, collecting fraction segments A-C, merging and concentrating, further performing Sephadex LH-20 gel column chromatography, eluting with methanol at a flow rate of 1-2 drops/second, collecting 2ml of a tube, collecting fractions with 2 column volumes (d=3cm and h=170cm), analyzing by TLC thin layer chromatography, merging 35-46 tubes, and concentrating to obtain fraction 2.
Ethyl acetate crude distillation section: the column is packed by ODS reverse phase silica gel packing, eluent is a methanol-water eluting system, the eluting gradient is that the volume ratio of methanol to water is 40:60, 50:50, 60:40, 70:40 and 100:0, each gradient elutes 2 column volumes (d=4cm, h=150cm), A-E5 distillation sections are sequentially obtained, the distillation sections A-C are collected, the distillation sections A-C are combined and concentrated, the mixture is further processed by Sephadex LH-20 gel column chromatography, the methanol is used for eluting, the flow rate is 1-2 drops/second, 2ml is used for collecting one tube, the fractions of 2 column volumes (d=3cm, h=170cm) are collected, TLC thin layer chromatography analysis is used for collecting 22-38 tubes, and fraction 3 is obtained after concentration.
Finally, mixing the fraction 1, the fraction 2 and the fraction 3 according to the mass ratio of 1:2.2:1.5 to obtain the angelica extract.
The preparation method of the glossy privet fruit extract comprises the following steps: pulverizing fructus Ligustri Lucidi, weighing methanol (70 wt%) of 10 times of fructus Ligustri Lucidi powder, soaking for 12 hr, reflux-extracting for 2 hr, concentrating the filtered filtrate to obtain extract, sequentially extracting with petroleum ether (3 times of extract mass) for 3 times, ethyl acetate (3 times of extract mass) for 3 times, and methanol (2 times of extract mass) for 3 times, filtering, concentrating to obtain petroleum ether section crude fraction, ethyl acetate section crude fraction, and methanol section crude fraction, and subjecting the ethyl acetate section crude fraction and methanol section crude fraction to column chromatography, wherein the method comprises the following steps:
ethyl acetate section crude fraction: packing a column by adopting ODS reverse phase silica gel packing, eluting with a methanol-water eluting system, eluting with methanol and water in the volume ratio of 40:60, 50:50, 60:40, 70:30 and 100:0, eluting 2 column volumes (d=4cm and h=150cm) per gradient, sequentially obtaining A-E6 distillation sections, collecting the distillation sections A-D, combining and concentrating, further performing Sephadex LH-20 gel column chromatography, eluting with methanol at the flow rate of 1-2 drops/sec, collecting 2ml of a tube, collecting fractions with 2 column volumes (d=3cm and h=170cm), performing TLC thin layer chromatography analysis, combining the 32-47 tubes, and concentrating to obtain fraction 1.
Methanol section crude fraction: packing a column by adopting ODS reverse phase silica gel packing, eluting with a methanol-water eluting system, eluting with methanol and water in the volume ratio of 35:65, 45:55, 55:45, 65:35 and 100:0, eluting 2 column volumes (d=4cm and h=150cm) per gradient, sequentially obtaining A-E6 distillation sections, collecting distillation sections B-D, combining and concentrating, further purifying by Sephadex LH-20 gel column chromatography, eluting with methanol at the flow rate of 1-2 drops/sec, collecting 2ml of a tube, collecting fractions with 2 column volumes (d=3cm and h=170cm), analyzing by TLC thin layer chromatography, combining 24-37 tubes, and concentrating to obtain fraction 2.
Finally, the fraction 1 and the fraction 3 are mixed according to the mass ratio of 1:0.8 to obtain the glossy privet fruit extract.
The preparation method of the drynaria rhizome extract comprises the following steps: soaking the drynaria rhizome powder in methanol (70 wt%) of 12 times of the drynaria rhizome powder, standing for 24h, filtering, adding 2 times of methanol (70 wt%) into filter residue, reflux-extracting for 3h, filtering, combining two times of filtering and concentrating to obtain extract, sequentially extracting 3 times with petroleum ether (3 times of the extract mass), extracting 3 times with ethyl acetate (3 times of the extract mass), extracting 3 times with methanol (2 times of the extract mass), respectively filtering and concentrating to obtain crude petroleum ether section fraction, crude ethyl acetate section fraction and crude methanol section fraction, subjecting the crude ethyl acetate section fraction to column chromatography, filling the column with ODS reverse phase silica gel, eluting with methanol-water with a volume ratio of methanol to water of 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 100:0, eluting 2 column volumes (d=4 cm, h=150 cm) each gradient, sequentially obtaining A-G7 fragments, collecting fragments B-D, combining and concentrating to obtain crude ethyl acetate section fraction, eluting with a thin-phase chromatography column chromatography (d=2 cm), eluting with a methanol-water column chromatography with a volume ratio of 20:80, 40:60, 50:50:50:30, 60:30:100:0, eluting with water volume ratio of methanol-2 cm, eluting with 2:2 m, eluting with water, eluting with a thin layer chromatography tube chromatography, and concentrating to obtain a thin layer chromatography, and concentrating the eluent, and concentrating to obtain the eluent.
The preparation method of the ginger extract comprises the following steps: cleaning rhizoma Zingiberis recens, mashing, soaking in 10 times of water for 24 hr, ultrasonic treating for 30min, filtering, soaking the residue in water of the same quality, ultrasonic treating for 30min, filtering, repeating the above steps for three times, mixing the three filtrates, and concentrating to obtain rhizoma Zingiberis recens extract.
The preparation method of the biota orientalis leaf extract comprises the following steps: cleaning, drying and crushing cacumen biotae, adding 12 times of hot water (90 ℃) for soaking for 6 hours, carrying out ultrasonic treatment for 30 minutes, filtering, and deproteinizing the filtrate, wherein the specific method comprises the following steps: mixing chloroform and n-butanol according to a volume ratio of 4:1, mixing polysaccharide according to a volume ratio of 1:1, shaking for 30min by a shaking table, centrifuging for 10min at 4000r/min, taking out supernatant, repeating the deproteinizing step for 10 times, combining the supernatant, dialyzing by a permeable membrane, obtaining the MWCO with 12 KD-14 KD, and concentrating to obtain the cacumen Platycladi extract.
The preparation method of the lotus seed extract comprises the following steps: cleaning and mashing the herba Trogopyri Dibotryis, adding ethanol (75 wt%) of 8 times of the herba Trogopyri Dibotryis, soaking for 12h, carrying out ultrasonic treatment for 30min, filtering, mixing filter residues with ethanol (75 wt%) of 5 times of the filter residues, carrying out reflux extraction for 4h, filtering, combining the two filtrates, concentrating to obtain a crude extract, extracting the crude extract with petroleum ether (3 times of the extract mass) for 3 times, extracting with ethyl acetate (3 times of the extract mass) for 3 times, extracting with methanol (2 times of the extract mass) for 3 times, respectively carrying out filtering and concentrating to obtain petroleum ether section crude fraction, ethyl acetate section crude fraction and methanol section crude fraction, discarding the methanol section crude fraction, combining the petroleum ether section crude fraction and the ethyl acetate section crude fraction, and concentrating to obtain the herba Trogopyri Dibotryis extract.
The preparation method of the chilli extract comprises the following steps: cleaning Capsici fructus, drying, pulverizing, soaking in methanol (70wt%) solvent with 6 times of the weight of Capsici fructus powder for 10 hr, ultrasound for 20min, filtering, repeating the above steps for 4 times, mixing filtrates obtained for 5 times, and concentrating to obtain Capsici fructus extract.
The preparation method of the citrus extract comprises the following steps: cutting citrus peel, adding distilled water, soaking for 12 hr at a feed-liquid ratio of 1:40 (g/ml), heating to 90deg.C, extracting for 3 hr, filtering, and drying the filtrate to obtain citrus extract.
The natural anti-hair loss composition prepared in this example 1 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the embodiment 1 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: 35.87, and the average values of the hair loss numbers after 15 days, 30 days, 45 days and 60 days are 29.85, 24.99, 16.96 and 9.72, respectively, so that the natural anti-hair loss composition prepared in example 1 has an anti-hair loss effect.
EXAMPLE 2 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 2 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 2.5 parts of polygonum multiflorum extract, 1.5 parts of angelica extract, 3.3 parts of glossy privet fruit extract, 0.5 part of drynaria extract, 2.1 parts of ginger extract, 1.7 parts of biota orientalis extract, 1.8 parts of saussurea involucrata extract, 0.1 part of capsicum extract and 1.8 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.1:1.5.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.2.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 2 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the embodiment 1 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: the average values of the hair loss numbers after the use on days 15, 30, 45 and 60 were 31.43, 23.96, 16.05 and 7.17, respectively, and it was found that the natural anti-hair loss composition prepared in example 2 had an anti-hair loss effect.
EXAMPLE 3 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 3 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica extract, 2 parts of glossy privet fruit extract, 0.8 part of drynaria extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 2.5 parts of saussurea involucrata extract, 0.8 part of capsicum extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 3 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the embodiment 1 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: the average values of the hair loss numbers after the use on days 15, 30, 45 and 60 were 31.8, 22.87, 13.55 and 5.46, respectively, and it was found that the natural anti-hair loss composition prepared in example 3 had an anti-hair loss effect.
EXAMPLE 4 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 4 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3 parts of polygonum multiflorum extract, 0.5 part of angelica extract, 2.4 parts of glossy privet fruit extract, 1.8 parts of drynaria extract, 2.5 parts of ginger extract, 2.2 parts of biota orientalis extract, 0.8 part of saussurea involucrata extract, 1.2 parts of capsicum extract and 0.9 part of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:0.5:1.5.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 4 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the embodiment 1 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: 37.05, and the average values of the hair loss numbers after 15 days, 30 days, 45 days and 60 days are 30.74, 24.11, 16.53 and 8.89, respectively, so that the natural anti-hair loss composition prepared in example 4 has an anti-hair loss effect.
EXAMPLE 5 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 5 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 5 parts of polygonum multiflorum extract, 1.1 parts of angelica extract, 1 part of glossy privet fruit extract, 1.4 parts of drynaria extract, 0.5 part of ginger extract, 2.5 parts of biota orientalis extract, 1.5 parts of saussurea involucrata extract, 1.8 parts of chilli extract and 0.5 part of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:2.2:0.5.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:2.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 5 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the embodiment 1 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: 36.21, and the average values of the hair loss numbers after 15 days, 30 days, 45 days and 60 days are 30.16, 25.17, 17.00 and 9.63, respectively, so that the natural anti-hair loss composition prepared in example 5 has an anti-hair loss effect.
EXAMPLE 6 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 6 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 2 parts of glossy privet fruit extract, 0.8 part of drynaria extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 2.5 parts of lotus seed extract, 0.8 part of chilli extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 6 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the embodiment 1 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: the average values of the hair loss numbers after the use on days 15, 30, 45 and 60 were 33.08, 36.4, 19.75 and 12.97, respectively, and it was found that the natural anti-hair loss composition prepared in example 6 had an anti-hair loss effect.
EXAMPLE 7 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 7 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica extract, 0.8 part of drynaria extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 2.5 parts of lotus seed extract, 0.8 part of chilli extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 7 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, were selected, and the natural hair loss prevention composition prepared in the example 7 was continuously used for 2 months, after every two days, after hair and scalp were cleaned, 2-5 mL each time was used, and was uniformly applied to hair and scalp, and kept for 2-3 minutes, and after rinsing, the hair loss number (root) after three times of hair and scalp cleaning was recorded and averaged before use: 35.48, and the average values of the hair loss numbers after 15 days, 30 days, 45 days and 60 days are 31.08, 25.46, 19.17 and 13.92, respectively, so that the natural anti-hair loss composition prepared in this example 7 has an anti-hair loss effect.
EXAMPLE 8 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 8 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica sinensis extract, 2 parts of glossy privet fruit extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 2.5 parts of eclipta alba extract, 0.8 part of capsicum extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 8 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, are selected, and the natural hair loss prevention composition prepared in the example 8 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times before use is recorded and is averaged: the average hair loss numbers after 36.54, 15, 30, 45 and 60 days of use were 30.69, 23.28, 15.18 and 6.04, respectively, which revealed that the natural anti-hair loss composition prepared in example 8 had poor anti-hair loss effect.
EXAMPLE 9 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 9 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica extract, 2 parts of glossy privet fruit extract, 0.8 part of drynaria extract, 1.8 parts of ginger extract, 2.5 parts of eclipta alba extract, 0.8 part of capsicum extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 9 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, were selected, and the natural hair loss prevention composition prepared in the example 9 was continuously used for 2 months, after every two days, after hair and scalp were cleaned, 2-5 mL each time was used, and was uniformly applied to hair and scalp, and kept for 2-3 minutes, and after rinsing, the hair loss number (root) after three times of hair and scalp cleaning was recorded and averaged before use: 39.14, and the average values of the hair loss numbers after 15 days, 30 days, 45 days and 60 days are 33.64, 26.73, 18.95 and 11, respectively, so that the natural anti-hair loss composition prepared in this example 9 has an anti-hair loss effect.
EXAMPLE 10 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 10 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica extract, 2 parts of glossy privet fruit extract, 0.8 part of drynaria extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 0.8 part of capsicum extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the capsicum extract is the same as in example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 10 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 patients with alopecia, 5 men and 5 women, with age of 25-45 years, healthy head skin, no allergy or scar and no local systemic other diseases are selected, and the natural anti-alopecia composition prepared in the example 10 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times is recorded before the use and is averaged: the average values of the hair loss numbers after 40.22 days, 15 days, 30 days, 45 days and 60 days were 35.62, 29.75, 23.35 and 18.34, respectively, and thus it was found that the natural anti-hair loss composition prepared in example 10 was poor in anti-hair loss effect.
EXAMPLE 11 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 11 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica sinensis extract, 2 parts of glossy privet fruit extract, 0.8 part of drynaria rhizome extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 2.5 parts of saussurea involucrata extract and 1.5 parts of citrus extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation of the citrus extract was consistent with example 1.
The natural anti-hair loss composition prepared in this example 11 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 patients with alopecia, 5 men and 5 women, with age of 25-45 years, healthy head skin, no allergy or scar and no local systemic other diseases are selected, and the natural anti-alopecia composition prepared in the example 11 is continuously used for 2 months, is used after cleaning hair and scalp every two days, is used for 2-5 mL each time, is uniformly smeared on the hair and scalp, is kept for 2-3 minutes, is washed, and the hair loss number (root) after cleaning the hair and scalp three times is recorded before the use and is averaged: the average values of the hair loss numbers after the use on days 15, 30, 45 and 60 were 33.47, 26.69, 19.77 and 13, respectively, and it was found that the natural anti-hair loss composition prepared in example 11 had an anti-hair loss effect.
EXAMPLE 12 preparation of Natural anti-alopecia composition
The natural anti-hair loss composition prepared in the embodiment 12 comprises the following specific components in parts by mass: the natural anti-hair loss composition comprises 3.5 parts of polygonum multiflorum extract, 1.2 parts of angelica extract, 2 parts of glossy privet fruit extract, 0.8 part of drynaria extract, 1.8 parts of ginger extract, 1.2 parts of biota orientalis extract, 2.5 parts of saussurea involucrata extract and 0.8 part of capsicum extract.
The preparation method of the polygonum multiflorum extract is consistent with example 1.
The preparation method of the angelica sinensis extract is identical to that of the example 1, wherein the mass ratio of the fraction 1 to the fraction 2 to the fraction 3 is 1:1.5:2.
The preparation method of the glossy privet fruit extract is the same as in the example 1, except that the mass ratio of the fraction 1 to the fraction 2 is 1:1.5.
The preparation method of the drynaria extract is the same as in example 1.
The preparation method of the ginger extract is the same as in example 1.
The preparation method of the biota orientalis extract was the same as in example 1.
The preparation method of the extract of the eclipta alba is consistent with example 1.
The preparation method of the capsicum extract is the same as in example 1.
The natural anti-hair loss composition prepared in this example 12 was subjected to an anti-hair loss effect test, and the specific test procedure was as follows:
10 hair loss patients with 25-45 years old, healthy head skin, no allergy or scar and no local systemic other diseases, 5 men and 5 women, were selected, and the natural hair loss prevention composition prepared in the example 12 was continuously used for 2 months, after every two days, after cleaning hair and scalp, 2-5 mL each time was used, and was uniformly applied to hair and scalp, and kept for 2-3 minutes, and after rinsing, the hair loss number (root) after three times of hair and scalp cleaning was recorded and averaged before use: the average hair loss numbers after 37.92 days, 15 days, 30 days, 45 days and 60 days were 32.88, 26.85, 19.9 and 13.08, respectively, and thus it was found that the natural anti-hair loss composition prepared in example 12 was poor in anti-hair loss effect.
The anti-hair loss compositions prepared in examples 1 to 12 of the present invention were subjected to related toxicology tests before evaluating the anti-hair loss effect thereof, and were rinsed by an acute eye irritation test (30 s), and as a result, were non-irritating; through an acute skin irritation test, the result shows that the skin irritation is free; the result of the skin phototoxicity test shows that no skin phototoxicity is seen; the skin allergy test shows that no skin allergy is found.
The anti-hair loss composition of the present invention was mixed with a general shampoo/shampoo product having only a cleansing effect in evaluating its anti-hair loss effect, wherein the anti-hair loss composition was added in an amount of 10% of the total mass of the mixture. The ingredients of conventional shampoos/shampoos may include the usual additives ingredients of lipids, humectants, surfactants, and water.
It should be noted that, in the present specification, specific features, structures, materials, or characteristics may be arbitrarily combined, and in order to simplify the description, all possible combinations of the features in the foregoing embodiments are not described, and those skilled in the art may combine and combine the features of the different embodiments and the different embodiments described in the present specification without contradiction.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (9)
1. A natural anti-hair loss composition, which is characterized by comprising polygonum multiflorum extract, angelica extract, glossy privet fruit extract, drynaria extract, ginger extract, biota orientalis extract, saussurea involucrata extract, capsicum extract and citrus extract.
2. The natural anti-hair loss composition according to claim 1, wherein the composition comprises, in parts by weight, 1 to 5 parts of polygonum multiflorum extract, 0.1 to 3 parts of angelica extract, 0.5 to 5 parts of glossy privet fruit extract, 0.1 to 2 parts of drynaria extract, 0.5 to 5 parts of ginger extract, 0.1 to 3 parts of biota orientalis extract, 0.1 to 3 parts of saussurea involucrata extract, 0.1 to 2 parts of capsicum extract, and 0.1 to 2 parts of citrus extract.
3. The natural anti-hair loss composition according to claim 1, wherein the polygonum multiflorum extract is prepared by the following steps: cleaning tuber fleeceflower root, drying, crushing, soaking in solvent, reflux extracting, filtering and concentrating to obtain extractum, stepped extracting with solvent, filtering and concentrating to obtain coarse fraction, column chromatographic treatment of the coarse fraction, and concentrating the eluted fraction to obtain tuber fleeceflower root extract.
4. The natural anti-hair loss composition according to claim 1, wherein the angelica extract is prepared by the following steps: soaking radix Angelicae sinensis powder in solvent, reflux extracting, filtering, concentrating to obtain extract, subjecting the extract to fractional extraction with solvent, filtering, concentrating to obtain crude fraction, subjecting the crude fraction to column chromatography, and concentrating the eluted fraction to obtain radix Angelicae sinensis extract.
5. The natural anti-hair loss composition according to claim 1, wherein the glossy privet fruit extract is prepared by the following method: pulverizing fructus Ligustri Lucidi, soaking in solvent, reflux extracting, filtering, concentrating to obtain extract, subjecting the extract to fractional extraction with solvent, filtering, concentrating to obtain crude fraction, subjecting the crude fraction to column chromatography, and concentrating the eluted fraction to obtain fructus Ligustri Lucidi extract.
6. The natural anti-hair loss composition according to claim 1, wherein the preparation method of the drynaria extract is as follows: soaking rhizoma Drynariae powder in solvent, reflux extracting, filtering, concentrating to obtain extract, subjecting the extract to fractional extraction with solvent, filtering, concentrating to obtain crude fraction, subjecting the crude fraction to column chromatography, and concentrating the eluted fraction to obtain rhizoma Drynariae extract.
7. The natural anti-hair loss composition according to claim 1, wherein the ginger extract is prepared by the process of: cleaning rhizoma Zingiberis recens, mashing, soaking in water, ultrasonic treating, filtering, soaking the residue in water of the same quality, ultrasonic treating, filtering, repeating the same steps for three times, mixing the three filtrates, and concentrating to obtain rhizoma Zingiberis recens extract; the preparation method of the biota orientalis leaf extract comprises the following steps: cleaning folium Platycladi, drying, pulverizing, soaking in hot water, ultrasonic treating, filtering, deproteinizing the filtrate, dialyzing, and concentrating to obtain folium Platycladi extract; the preparation method of the lotus seed extract comprises the following steps: cleaning herba Saussureae Involueratae, mashing, soaking in solvent, ultrasonic treating, filtering, reflux extracting, filtering, mixing filtrates, concentrating to obtain crude extract, respectively extracting with solvent, filtering, mixing filtrates, and concentrating to obtain herba Saussureae Involueratae extract; the preparation method of the chilli extract comprises the following steps: cleaning capsicum, drying, pulverizing, soaking in solvent, ultrasonic treating, filtering, repeating the steps for 3-5 times, mixing filtrates, and concentrating to obtain capsicum extract; the preparation method of the citrus extract comprises the following steps: cutting citrus peel, soaking in distilled water, heat extracting, filtering, and drying to obtain citrus extract.
8. Use of a natural anti-hair loss composition according to any one of claims 1 to 7 in scalp, hair or hair follicle repair care products.
9. The use according to claim 8, wherein the scalp, hair or hair follicle repair care product is in any form of liquid, cream, emulsion, foam or spray.
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