CN116391649A - Method for fully artificial cultivation of parent clown fish by gonad maturation promotion - Google Patents

Method for fully artificial cultivation of parent clown fish by gonad maturation promotion Download PDF

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CN116391649A
CN116391649A CN202310335603.6A CN202310335603A CN116391649A CN 116391649 A CN116391649 A CN 116391649A CN 202310335603 A CN202310335603 A CN 202310335603A CN 116391649 A CN116391649 A CN 116391649A
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clown
parts
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CN116391649B (en
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何丽斌
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Fisheries Research Institute Of Fujian (fujian Aquatic Disease Prevention Center)
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Abstract

The invention provides a method for fully artificial cultivation of clown fish parent fish by gonad maturation promotion, which comprises the following steps: s1, when young fish of artificially bred clown fish grow to 1.5-2cm, healthy young fish is selected as parent fish breeding population; s2, carrying out group culture on parent fish culture groups in the same batch, feeding feed 1, wherein the feeding amount is 1.5-3% of the mass of the fish body, and the density is controlled to be 1.0-1.8 tail/L; s3, after 150-160 days of age, selecting clown fishes with a proportion of male to female of 1:1 for pairing, separately isolating and culturing, feeding feed 2, wherein the feeding amount is 2.5-3.5% of the mass of the fish body; s4, feeding feed 3 every 15 days between 180 and 210 days old, feeding feed 4 every 10 days old and feeding feed 5 every 7 days old after 240 days old for sexual maturity; s5, after ripening cultivation for 3-5 months, feeding feed 6 when the clown fish shows signs before spawning and the abdomen of the female fish gradually swells and rises, and the clown fish at this time is the parent fish of the clown fish. The method can realize the complete artificial cultivation of the parent fish of the clown fish.

Description

Method for fully artificial cultivation of parent clown fish by gonad maturation promotion
Technical Field
The invention relates to a method for fully artificial cultivation of parent fish of clown fish by gonad maturation promotion, belonging to the technical field of clown fish reproduction.
Background
Clown fish (also called sea anemone fish (belonging to the family of the sparrow (Pomacntridae) (sea anemone fish belongs to the genus Amphion (30 species are shared worldwide, and the sea reef is seriously damaged due to artificial miscatch, the sea reef is severely damaged due to the fact that the color is beautiful, and the attractive value of people is extremely high).
In summary, the prior art lacks the clown fish parent fish.
Disclosure of Invention
The invention provides a method for fully artificial cultivation of parent clown fish with gonad maturation promotion, which can effectively solve the problems
The invention is realized in the following way: .
A method for fully artificial cultivation of clown fish parent fish by gonad maturation promotion comprises the following steps:
s1, when young fish of artificially bred clown fish grow to 1.5-2cm, healthy young fish is selected as parent fish breeding population;
s2, group-culturing parent fish culture groups in the same batch, feeding feed 1, wherein the feed is fed once every two hours from 6 am to 6 pm every day, and the total time is 7 times; the feeding amount of each time is 1.5-3% of the fish body mass, and the density is controlled to be 1.0-1.8 tail/L;
s3, after 150-160 days of age, selecting clown fish with a proportion of male to female of 1:1 for pairing, separately isolating and cultivating, feeding feed 2, and feeding 7 times every two hours from 6 am to 6 pm every day; the feeding amount of each time is 2.5-3.5% of the fish body;
s4, feeding feed 3 every 15 days between 180 and 210 days old, feeding feed 4 every 10 days old and feeding feed 5 every 7 days old after 240 days old for sexual maturity; feeding the feed 2 at other times, wherein the feeding is performed for 7 times every two hours from 6 am to 6 pm every day; the feeding amount of each time is 2.5-3.5% of the fish body;
s5, after ripening cultivation for 3-5 months, when the clown fish shows signs before spawning and the abdomen of the female fish gradually swells and rises, feeding a feed 6 once, wherein the clown fish is the parent fish of the clown fish; feeding the feed 2 at other times, wherein the feeding is performed for 7 times every two hours from 6 am to 6 pm every day; the feeding amount of each time is 2.5-3.5% of the fish body mass.
Feed 1: 40-50 parts of fish meal, 15-25 parts of kelp powder, 5-10 parts of alpha starch, 1.5-2.5 parts of soybean lecithin, 16-18 parts of artemia powder, 1-1.5 parts of chitosan and 3-5 parts of DHA algae powder. Antarctic krill powder: 10-15 parts of bile acid salt 0.08-0.15 part of betaine 0.04-0.045 part of compound microecological preparation 0.04-0.06 part of feed, wherein the feed is granules with the grain diameter of 1-2.5mm;
feed 2: 40-55 parts of shrimp meat, 10-15 parts of oyster meat and clam meat: 10-15 parts of fish meal, 5-10 parts of alpha-starch, 8-10 parts of multi-vitamin multi-mineral, 2 parts of spirulina, 8-10 parts of euphausia superba powder: 8-12 parts of compound microecological preparation 0.05-0.08 part, and preparing feed into granules with the particle size of 1.5-3mm;
feed 3: LHRH-A 2 (45-55 g)/100 g of feed 2;
feed 4: LHRH-A 2 (70-80 g)/100 g feed 2;
feed 5: LHRH-A 2 (95-105 g)/100 g feed 2;
feed 6: (LHRH-A) 3 400-500g+HCG:14000-16000IU+DOM:175-185 mg)/100 g of feed 2;
the composite microecological preparation consists of lactobacillus, bacillus subtilis and saccharomycetes according to the mass ratio of the mixing ratio of 1:1:1, and the content of the mixed strain is 1 multiplied by 10 6 cfu/g-1×10 9 cfu/g；
And (3) water quality management: culturing in a circulating water mode, wherein the PH of the seawater is 8.0-8.2, and the mass concentration of ammonia nitrogen is lower than 0.05mg/L; the dissolved oxygen is higher than 5.5mg/L, food residues and feces are sucked off at 10 am and 4 pm every day, and water is changed for 30% every week.
In the prior art, LHRH-A 2 、LHRH-A 3 HCG and DOM are commonly used as oxytocic, and artificial injection of sexually mature fish to promote oestrus and ovulation of fish and increase reproductive efficiency has not been reported for the use of these hormones as fish feed and for immature fish. In the application, the hormones are firstly made into fish feed, and the fish feed is fed when the clown fish is not sexually mature, so that the gonad development maturation of the clown fish is promoted, and the sexual maturation time of the clown fish is shortened.
As a further improvement, in the step S2, the water temperature is stabilized at (26 (1 (DEG C, salinity 30-32; pH8.0-8.2, dissolved oxygen not less than 5.5mg/L, ammonia nitrogen not less than 0.05mg/L, illumination period 12L:12D, illumination intensity 7000lx, illumination time 6:00-18:00, water flow during feeding and night is controlled at 4500L/H, and water flow during other times is controlled at 10000L/H).
As a further improvement, in the step S3, the water temperature is stabilized at (26 (1 (DEG C, salinity of 30-32; pH of 8.0-8.2, dissolved oxygen of more than or equal to 5.5mg/L, ammonia nitrogen of less than 0.05mg/L, illumination period of 12L:12D, illumination intensity of 5000lx, illumination time of 6:00-18:00, water flow during feeding and night is controlled at 6000L/H, and water flow during other times is controlled at 12000L/H).
As a further improvement, in the step S4, the water temperature is stabilized at (26 (1 (DEG C, salinity of 30-32; pH of 8.0-8.2, dissolved oxygen of more than or equal to 5.5mg/L, ammonia nitrogen of less than 0.05mg/L, illumination period of 12L:12D, illumination intensity of 5000lx, illumination time of 6:00-18:00, water flow during feeding and night is controlled at 6000L/H, and water flow during other times is controlled at 12000L/H).
As a further improvement, in the step S5, the water temperature is stabilized at (26 (1 (DEG C, salinity of 30-32; pH of 8.0-8.2, dissolved oxygen of more than or equal to 5.5mg/L, ammonia nitrogen of less than 0.05mg/L, illumination period of 12L:12D, illumination intensity of 5000lx, illumination time of 6:00-18:00, water flow during feeding and night is controlled at 6000L/H, and water flow during other times is controlled at 12000L/H).
As a further improvement, in step S3, the pairing employs cross pairing between different parents.
As a further improvement, on the day of feeding feeds 3, 4, 5, 6, any other feed should be taken off, only on 9 am on the day: and feeding twice at 00 hours and 14:00 pm.
The beneficial effects of the invention are as follows:
according to the method for cultivating the parent fish of the clown fish, artificially bred clown fish is adopted, feeds and growth conditions of each growth stage are adjusted according to different nutrition requirements and growth environment requirements of different growth stages, nutrition and growth environments in natural ecological environments are simulated to the greatest extent, growth and development conditions of the clown fish are improved, meanwhile, hormone feeds are used for ripening when the clown fish is not sexually mature, gonad development and maturation of the clown fish are promoted, the bred clown fish is good in growth and development conditions and early in sexual maturity, the method can be used as the parent fish of the clown fish, namely, artificial clown fish with poor growth and development conditions can be cultivated into the parent fish of the clown fish with good growth and development conditions, and the complete artificial cultivation of the parent fish of the clown fish is successfully realized.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
Example 1
A method for fully artificial cultivation of clown fish parent fish by gonad maturation promotion comprises the following steps:
s1, when young fish of the artificially bred clown fish grow to 1.5-2cm (35-40 days old), healthy young fish is selected as a parent fish breeding population. The propagation method of the artificial propagation clown fish comprises the following steps: taking wild clown fish (purchased from a Guangzhou sea pearl area Yongfeng aquarium), feeding the wild clown fish in a glass aquarium, collecting fertilized eggs for hatching, after young fish hatching, feeding rotifers from the time of young fish hatching to the time of 7 days; feeding rotifers and artemia at 7-15 days of age; feeding copepods and common powder feed at 15-35 days of age; after 35 days of age, pellet feed 1 was fed. The feeding time is as follows: feeding is carried out every two hours from 6 am to 6 pm every day. The water temperature is stabilized at (26+/-1 (DEG C, salinity is 25-28; pH is 8.0-8.2), dissolved oxygen is more than or equal to 3.5mg/L, ammonia nitrogen is less than 0.1mg/L, illumination period is 12L:12D, illumination intensity is 4000lx, illumination time is 6:00-18:00, and water flow is controlled at 8000L/H.
S2, group-culturing parent fish culture groups in the same batch, feeding feed 1, wherein the feed is fed once every two hours from 6 am to 6 pm every day, and the total time is 7 times; the feeding amount of each time is 1.5-3% of the fish body mass, and the density is controlled to be 1.0-1.8 tail/L; in the step S2, the water temperature is stabilized at (26 (1 (DEG C, salinity 30-32; pH8.0-8.2, dissolved oxygen not less than 5.5mg/L, ammonia nitrogen not less than 0.05mg/L, illumination period 12L:12D, illumination intensity 7000L x, illumination time 6:00-18:00, water flow during feeding and night is controlled at 4500L/H, and water flow at 10000L/H at other times).
S3, after 150-160 days of age, selecting clown fish with a proportion of male to female of 1:1 for pairing, separately isolating and cultivating, feeding feed 2, and feeding 7 times every two hours from 6 am to 6 pm every day; the feeding amount of each time is 2.5-3.5% of the fish body; the pairing adopts cross pairing among different parents, and the close-breeding is avoided so as to ensure the excellent characters of the offspring. In the step S3, the water temperature is stabilized at (26 (1 (DEG C, salinity is 30-32, pH is 8.0-8.2, dissolved oxygen is more than or equal to 5.5mg/L, ammonia nitrogen is less than 0.05mg/L, illumination period is 12L:12D, illumination intensity is controlled at 5000lx, illumination time is 6:00-18:00, water flow is controlled at 6000L/H during feeding and night, and water flow is controlled at 12000L/H at other times).
S4, feeding feed 3 every 15 days between 180 and 210 days old, and feeding feed 4 every 10 days between 210 and 240 days old; feed was fed 5 to sexual maturity every 7d after 240 days of age. On the day of feeding feeds 3, 4, 5, any other feed should be taken off, only on the day 9 am: the fish is fed twice at 00 hours and 14:00 pm, so that the hormone feed is guaranteed to be ingested by the fish completely to absorb the nutrition components better; feeding the feed 2 at other times, wherein the feeding is performed for 7 times every two hours from 6 am to 6 pm every day; the feeding amount of each time is 2.5-3.5% of the fish body mass. In the step S4, the water temperature is stabilized at (26 (1 (DEG C, salinity is 30-32, pH is 8.0-8.2, dissolved oxygen is more than or equal to 5.5mg/L, ammonia nitrogen is less than 0.05mg/L, illumination period is 12L:12D, illumination intensity is controlled at 5000lx, illumination time is 6:00-18:00, water flow is controlled at 6000L/H during feeding and night, and water flow is controlled at 12000L/H at other times).
S5, after ripening cultivation for 3-5 months, when the clown fish shows signs before spawning and the abdomen of the female fish gradually swells and rises, feeding the feed 6 once, wherein the feeding amount is 1.5-2.0% of the mass of the fish body, and the feeding time and the feeding method are the same as those of the feed 3. The clown fish cultivated in the step is the clown fish parent fish. In the step S5, the water temperature is stabilized at (26 (1 (DEG C, salinity is 30-32, pH is 8.0-8.2, dissolved oxygen is more than or equal to 5.5mg/L, ammonia nitrogen is less than 0.05mg/L, illumination period is 12L:12D, illumination intensity is controlled at 5000lx, illumination time is 6:00-18:00, water flow is controlled at 6000L/H during feeding and night, and water flow is controlled at 12000L/H at other times).
Collecting fertilized eggs after spawning of parent fish of clown fish, placing fertilized eggs entering a hatching period into a hatching barrel filled with seawater, adjusting water flow to simulate parent fish egg protection to enable the fertilized eggs to swing slightly for hatching, wherein the temperature of the seawater is 26-28 ℃, the salinity is 28-30, the pH is 8.0-8.2, the dissolved oxygen is more than or equal to 5.5mg/L, and the ammonia nitrogen is less than 0.05mg/L. Clown fish generally hatching in the dark, and the hatching barrel is kept away from light during hatching.
Feed 1: 40-50 parts of fish meal, 15-25 parts of kelp powder, 5-10 parts of alpha starch, 1.5-2.5 parts of soybean lecithin, 16-18 parts of artemia powder, 1-1.5 parts of chitosan and 3-5 parts of DHA algae powder. Antarctic krill powder: 10-15 parts of bile acid salt 0.08-0.15 part of betaine 0.04-0.045 part of compound microecological preparation 0.04-0.06 part of feed, wherein the feed is granules with the grain diameter of 1-2.5mm;
feed 2: 40-55 parts of shrimp meat, 10-15 parts of oyster meat and clam meat: 10-15 parts of fish meal, 5-10 parts of alpha-starch, 8-10 parts of multi-vitamin multi-mineral, 2 parts of spirulina, 8-10 parts of euphausia superba powder: 8-12 parts of compound microecological preparation 0.05-0.08 part, and preparing feed into granules with the particle size of 1.5-3mm;
feed 3: LHRH-A 2 (50g) 100g of feed 2;
feed 4: LHRH-A 2 (75g) 100g of feed 2;
feed 5: LHRH-A 2 (100g) 100g of feed 2;
feed 6: (LHRH-A) 3 450 g+HCG:15000IU+DOM:180mg)/100 g of feed 2;
the composite microecological preparation consists of lactobacillus, bacillus subtilis and saccharomycetes according to the mass ratio of the mixing ratio of 1:1:1, and the content of the mixed strain is 1 multiplied by 10 6 cfu/g-1×10 9 cfu/g；
And (3) water quality management: culturing in a circulating water mode, wherein the PH of the seawater is 8.0-8.2, and the mass concentration of ammonia nitrogen is lower than 0.05mg/L; the dissolved oxygen is higher than 5.5mg/L, food residues and feces are sucked off at 10 am and 4 pm every day, and water is changed for 30% every week.
Three batches of clown fish parent fish are cultivated according to the same method, and one batch is as follows: the double-saw fish with the Kirschner is white strips in one batch, and the double-saw fish with the Kirschner is: clown fish with black princess. Each batch of clown fish 30 pairs.
The spawning amount of the clown fish, the hatching rate and the survival rate of fertilized eggs (90 days after the hatching of fertilized eggs) and the pairing time of parent fish (the time from the hatching of the young fish to the first spawning) are counted. The experimental results are shown in table 1.
TABLE 1
Kirschner double saw fish White strip double saw fish Clown fish with black princess
Egg laying amount (particle @ of 1500-1950 1050-1550 850-1000
Hatching rate (% 90.41(3.17 89.06(2.49 90.27(5.30
Survival rate (%) of 76.21(3.16 78.26(8.03 73.68(5.43
Pairing time (d) 346.01(23.14 350.14(23.07 343.27(19.07
Comparative example 1
The difference from example 1 is that: the feeding of feeds 3, 4, 5 and 6 was changed to feed 2, i.e. only feeds 1 and 2 were fed, and no hormone feeds 3, 4, 5 and 6 were fed, otherwise the same as in example 1. The experimental results are shown in table 2.
TABLE 2
Kirschner double saw fish White strip double saw fish Clown fish with black princess
Egg laying amount (particle @ of 1250-1700 850-1150 700-950
Hatching rate (% 85.21(6.32 86.04(5.13 84.73(5.07
Survival rate (%) of 68.34(4.23 66.62(5.34 67.35(4.66
Pairing time (d #) 437.46(31.62 438.41(26.63 448.67(16.77
Comparative example 2
The difference from example 1 is that: the procedure of example 1 was repeated except that the feeds 1 and 2 were each a pellet of ordinary feed (Xiamen Huapu aquatic products development Co., ltd., sample series feed) and the hormone feeds 3, 4, 5 and 6 were thrown. The experimental results are shown in table 3.
TABLE 3 Table 3
Kirschner double saw fish White strip double saw fish Clown fish with black princess
Egg laying amount (particle @ of 1000-1450 650-900 500-750
Hatching rate (% 56.18(13.51 53.41(10.53 63.12(8.09
Survival rate (%) of 48.01(6.33 46.71(5.60 46.44(7.08
Pairing time (d #) 388.24(26.02 390.43(20.17 396.12(23.10
Comparative example 3
The difference from example 1 is that: the feeding feeds 1, 2, 3, 4, 5 and 6 were all ordinary feed pellets (Xiamen Huapu aquatic development Co., ltd., sample series feeds), i.e., the feeds 1 and 2 were not fed, and the hormone feeds 3, 4, 5 and 6 were not fed, and the other operations were the same as in example 1. Experimental results show that at 600d, each batch of clown fish is sexually mature.
From the viewpoint of spawning amount, example 1 was larger than comparative examples 1, 2, and 3; from the time of pairing, the time of pairing of the hormone-added example 1 and comparative example 3 is significantly shorter than that of comparative example 2 without hormone addition; the hatchability and survival rate of fertilized eggs of example 1 and comparative example 2 using feeds 1 and 2 were both higher than those of comparative example 2 using ordinary feeds. The high nutrition feed and hormone feed of the embodiment 1 are matched with each other, so that the gonad maturation of the clown fish can be promoted, and the artificial clown fish can be cultivated into qualified parent fish with stable growth state and good development.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. A method for fully artificial cultivation of parent fish of clown fish by gonad maturation promotion, which is characterized by comprising the following steps:
s1, when young fish of artificially bred clown fish grow to 1.5-2cm, healthy young fish is selected as parent fish breeding population;
s2, group-culturing parent fish culture groups in the same batch, feeding feed 1, wherein the feed is fed once every two hours from 6 am to 6 pm every day, and the total time is 7 times; the feeding amount of each time is 1.5-3% of the fish body mass, and the density is controlled to be 1.0-1.8 tail/L;
s3, after 150-160 days of age, selecting clown fish with a proportion of male to female of 1:1 for pairing, separately isolating and cultivating, feeding feed 2, and feeding 7 times every two hours from 6 am to 6 pm every day; the feeding amount of each time is 2.5-3.5% of the fish body;
s4, feeding feed 3 every 15 days between 180 and 210 days old, feeding feed 4 every 10 days old and feeding feed 5 every 7 days old after 240 days old for sexual maturity;
s5, after ripening cultivation for 3-5 months, feeding a feed 6 when the clown fish shows signs before spawning and the abdomen of the female fish gradually swells and rises, wherein the clown fish is the parent fish of the clown fish;
feed 1: 40-50 parts of fish meal, 15-25 parts of kelp powder, 5-10 parts of alpha starch, 1.5-2.5 parts of soybean lecithin, 16-18 parts of artemia powder, 1-1.5 parts of chitosan and 3-5 parts of DHA algae powder. Antarctic krill powder: 10-15 parts of bile acid salt 0.08-0.15 part of betaine 0.04-0.045 part of compound microecological preparation 0.04-0.06 part of feed, wherein the feed is granules with the grain diameter of 1-2.5mm;
feed 2: 40-55 parts of shrimp meat, 10-15 parts of oyster meat and clam meat: 10-15 parts of fish meal, 5-10 parts of alpha-starch, 8-10 parts of multi-vitamin multi-mineral, 2 parts of spirulina, 8-10 parts of euphausia superba powder: 8-12 parts of compound microecological preparation 0.05-0.08 part, and preparing feed into granules with the particle size of 1.5-3mm;
feed 3: LHRH-A 2 (45-55 g)/100 g of feed 2;
feed 4: LHRH-A 2 (70-80 g)/100 g feed 2;
feed 5: LHRH-A 2 (95-105 g)/100 g feed 2;
feed 6: (LHRH-A) 3 400-500g+HCG:14000-16000IU+DOM:175-185 mg)/100 g of feed 2;
the composite microecological preparation consists of lactobacillus, bacillus subtilis and saccharomycetes according to the mass ratio of the mixing ratio of 1:1:1, and the content of the mixed strain is 1 multiplied by 10 6 cfu/g-1×10 9 cfu/g；
And (3) water quality management: culturing in a circulating water mode, wherein the PH of the seawater is 8.0-8.2, and the mass concentration of ammonia nitrogen is lower than 0.05mg/L; the dissolved oxygen is higher than 5.5mg/L, food residues and feces are sucked off at 10 am and 4 pm every day, and water is changed for 30% every week.
2. The method for fully artificial culture of clown fish parent fish with gonad-accelerated maturation according to claim 1, characterized in that in step S2, the water temperature is stabilized at 26 (1 (c, salinity 30-32; ph8.0-8.2, dissolved oxygen not less than 5.5mg/L, ammonia nitrogen <0.05mg/L, illumination period 12L:12d, illumination intensity 6000-8000L x, illumination time 6:00-18:00, water flow during feeding and night is controlled at 5000L/H, water flow at other times is controlled at 10000L/H).
3. The method for fully artificial culture of clown fish parent fish with gonad-accelerated maturation according to claim 1, wherein in the step S3, the water temperature is stabilized at 26 (1 (c, salinity 30-32, ph8.0-8.2, dissolved oxygen not less than 5.5mg/L, ammonia nitrogen <0.05mg/L, illumination period 12L:12d, illumination intensity controlled at 4000-6000lx, illumination time 6:00-18:00, water flow during feeding and night controlled at 8000L/H, and water flow at 12000L/H at other times.
4. The method for fully artificial culture of clown fish parent fish with gonad-accelerated maturation according to claim 1, wherein in step S4, the water temperature is stabilized at 26 (1 (°c, salinity 30-32, ph8.0-8.2, dissolved oxygen not less than 5.5mg/L, ammonia nitrogen <0.05mg/L, illumination period 12L:12d, illumination intensity controlled at 4000-6000lx, illumination time 6:00-18:00, feeding and night water flow controlled at 8000L/H, and other time water flow controlled at 12000L/H.
5. The method for fully artificial culture of clown fish parent fish with gonad-accelerated maturation according to claim 1, wherein in step S5, the water temperature is stabilized at 26 (1 (c, salinity 30-32, ph8.0-8.2, dissolved oxygen not less than 5.5mg/L, ammonia nitrogen <0.05mg/L, illumination period 12L:12d, illumination intensity controlled at 4000-6000lx, illumination time 6:00-18:00, water flow during feeding and night controlled at 8000L/H, and water flow at 12000L/H at other times.
6. The method for fully artificial cultivation of parent clown fish with gonad-accelerated maturation according to claim 1, wherein in step S3, the pairing is cross-paired between different parents.
7. The method for fully artificial cultivation of parent clown fish with gonad maturation according to claim 1, characterized in that on the day of feeding 3, 4, 5, 6 any other feed should be taken off, only on 9 am on the day: and feeding twice at 00 hours and 14:00 pm.
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101869085A (en) * 2010-06-22 2010-10-27 中国水产科学研究院南海水产研究所热带水产研究开发中心 Method for realizing high-yield clown fish parent fish culture in running water
CN101878743A (en) * 2010-06-22 2010-11-10 中国水产科学研究院南海水产研究所热带水产研究开发中心 Method for totally-artificial batch culture of parent fish of clown fish
CN102613113A (en) * 2012-03-09 2012-08-01 海南大学 Method for increasing body colors of artificially-bred anemone fish of coral reef fish
CN103907552A (en) * 2014-01-13 2014-07-09 中国科学院南海海洋研究所 Method for preventing and controlling copepods in young sea cucumber breeding pond of tropical sea cucumbers
CN104170777A (en) * 2014-08-06 2014-12-03 浙江华兴水产科技有限公司 Breeding method for amphiprion ocellarises
CN107136349A (en) * 2017-05-24 2017-09-08 福建省水产研究所 A kind of formula of the hyperchromic feed of red clown fish and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101869085A (en) * 2010-06-22 2010-10-27 中国水产科学研究院南海水产研究所热带水产研究开发中心 Method for realizing high-yield clown fish parent fish culture in running water
CN101878743A (en) * 2010-06-22 2010-11-10 中国水产科学研究院南海水产研究所热带水产研究开发中心 Method for totally-artificial batch culture of parent fish of clown fish
CN102613113A (en) * 2012-03-09 2012-08-01 海南大学 Method for increasing body colors of artificially-bred anemone fish of coral reef fish
CN103907552A (en) * 2014-01-13 2014-07-09 中国科学院南海海洋研究所 Method for preventing and controlling copepods in young sea cucumber breeding pond of tropical sea cucumbers
CN104170777A (en) * 2014-08-06 2014-12-03 浙江华兴水产科技有限公司 Breeding method for amphiprion ocellarises
CN107136349A (en) * 2017-05-24 2017-09-08 福建省水产研究所 A kind of formula of the hyperchromic feed of red clown fish and preparation method thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
司和;蒲利云;唐贤明;杨明秋;刘天密;王国福;: "透红小丑鱼促熟培育及养殖生态技术研究", 现代农业科技, no. 10 *
尤宏争;夏苏东;刘克明;刘义;李楠;王永辰;: "天津地区公子小丑鱼繁育技术初探", 中国水产, no. 07, 5 July 2016 (2016-07-05) *
鲍鹰;张鹏;祝承勇;王伟;刘鸣;: "红小丑人工繁殖和育苗的初步研究", 海洋科学, no. 02, 9 February 2009 (2009-02-09) *

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