CN116349775A - Feed additive for improving pig reproduction, immunity and disease resistance and preparation method thereof - Google Patents
Feed additive for improving pig reproduction, immunity and disease resistance and preparation method thereof Download PDFInfo
- Publication number
- CN116349775A CN116349775A CN202310212607.5A CN202310212607A CN116349775A CN 116349775 A CN116349775 A CN 116349775A CN 202310212607 A CN202310212607 A CN 202310212607A CN 116349775 A CN116349775 A CN 116349775A
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- chinese medicine
- traditional chinese
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- feed additive
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
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- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- C—CHEMISTRY; METALLURGY
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/225—Lactobacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a feed additive for improving pig reproduction, immunity and disease resistance and a preparation method thereof. The traditional Chinese medicine feed additive is prepared by mixing and fermenting a traditional Chinese medicine composition and a fermentation microbial inoculum; the traditional Chinese medicine composition consists of dangshen, angelica and white paeony root, and the zymophyte agent consists of saccharomycetes and lactobacillus. The invention combines the codonopsis pilosula, the angelica and the white paeony root into a traditional Chinese medicine compound, so that the compound traditional Chinese medicine has the effects of tonifying middle-jiao and Qi, resisting bacteria and inflammation, resisting oxidation, enhancing organism immunity and the like, and the mixed fermentation effect is obvious by adopting the saccharomycetes and lactobacillus with synergistic effect as the fermentation inoculant, has the advantages of improving the content of the active ingredients after the traditional Chinese medicine, the probiotics and the fermentation and the like, can prevent deficiency of Qi and blood of the sows in the gestation period after the administration of the sows in the latter period, effectively improves the growth performance, the oxidation resistance and the disease resistance of piglets produced by the pregnant sows, has exact prevention effect on diarrhea of the piglets, and can effectively reduce the incidence rate of diarrhea of the piglets.
Description
Technical Field
The invention relates to a traditional Chinese medicine feed additive for improving reproductive performance, immune function and disease resistance of livestock, in particular to a traditional Chinese medicine fermented feed additive for improving reproductive performance of sows and enhancing immune function and disease resistance of piglets and a preparation method thereof, belonging to the field of traditional Chinese medicine feed additives for improving reproductive performance, immune function and disease resistance of livestock.
Background
Diarrhea of piglets is a common disease in pig industry, once the piglets are infected with the disease in a growth stage, the survival rate of the piglets is seriously affected, the diarrhea of the piglets often occurs in the piglets in the first week of birth, antibiotic treatment is often not ideal, and the intestinal development of the piglets is imperfect, the digestion and absorption capacity is weak, and the intestinal tracts of the piglets are difficult to digest and absorb due to the fact that the traditional Chinese medicine preparation is directly fed, so that the treatment effect is affected.
In the fermentation process of the microbial fermentation traditional Chinese medicine, enzymes produced by various microorganisms can enable effective macromolecular substances of the traditional Chinese medicine to be absorbed and degraded into micromolecular substances which are easier to be absorbed by animals. In addition, various macromolecular impurities are removed, so that the drug effect is improved. The related researches show that the enzyme substances can interact with certain special components in the traditional Chinese medicine, and modify the active components to finally form new substances, and can also reduce the content of toxic components in the traditional Chinese medicine.
In large-scale breeding, the sow has insufficient qi and blood, the constitution of the piglets after birth is generally poor, and the incidence rate and the death rate of diarrhea of the piglets are high. The theory of "Su Wen-Siqi regulating spirit" states that "the infant under milk has diseases and must regulate her mother, and the mother transmits the medicine with breast milk An Zian". Traditional Chinese veterinarians have proposed "no disease to treat" as early as a thousand years ago, emphasizing that prevention is more important than treatment.
Based on the current research situation of piglet diarrhea, and combining the theory of middle veterinarian theory, method, prescription and medicine and the method of dialectical treatment of middle veterinarian, from the perspective of young disease treatment, if the traditional Chinese medicine fermentation preparation for enhancing the reproductive performance of pregnant sows, improving the immunity and disease resistance of piglets can be provided, the growth performance and disease resistance of piglets can be effectively improved when the traditional Chinese medicine fermentation preparation is administered in the later period of the pregnancy of the sows, the traditional Chinese medicine fermentation preparation has a certain prevention effect on piglet diarrhea, and the incidence rate of piglet diarrhea is obviously reduced.
Disclosure of Invention
The invention aims at providing a traditional Chinese medicine feed additive or a traditional Chinese medicine fermented feed additive for feeding pregnant sows to enhance reproductive performance of the pregnant sows and improve immunity and disease resistance of piglets.
The second object of the invention is to provide a method for preparing the traditional Chinese medicine feed additive composition or the traditional Chinese medicine fermented feed additive.
The third purpose of the invention is to apply the traditional Chinese medicine feed additive or the traditional Chinese medicine fermented feed additive to aspects of enhancing reproductive performance of pregnant sows, improving immunity or disease resistance of piglets born by the pregnant sows and the like.
In order to achieve the above purpose, the technical scheme adopted by the invention comprises the following steps:
the invention provides a traditional Chinese medicine feed additive for enhancing reproductive performance of pregnant sows and improving immunity and disease resistance of piglets, which consists of radix codonopsis pilosulae, angelica and white paeony root; preferably, the weight parts of the components are as follows: 0.5-4 parts of radix codonopsis pilosulae, 0.5-4 parts of angelica, and 0.5-4 parts of white peony root; more preferably, the weight parts of the components are: 1 part of radix codonopsis pilosulae, 1 part of angelica, and 1 part of white peony root.
The invention also provides a traditional Chinese medicine fermented feed additive for feeding pregnant sows to enhance reproductive performance of the pregnant sows and improve immunity and disease resistance of piglets, which comprises the following components: a traditional Chinese medicine composition, a fermentation microbial inoculum and a fermentation medium;
the traditional Chinese medicine composition consists of dangshen, angelica and white paeony root, and preferably comprises the following components in parts by weight: 0.5-4 parts of radix codonopsis pilosulae, 0.5-4 parts of angelica, and 0.5-4 parts of white peony root; more preferably, the weight parts of the components are: 1 part of radix codonopsis pilosulae, 1 part of angelica and 1 part of white peony root;
as a preferred embodiment of the invention, the fermentation broth is composed of saccharomycetes and lactobacillus.
In yet another aspect, the present invention provides a method for preparing the Chinese medicinal composition, comprising: extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water as extraction solvent under heating, filtering the extractive solution, and collecting supernatant.
As a preferred embodiment, the preparation method comprises extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with 8-12 times of water as extraction solvent, boiling, filtering, and collecting supernatant.
In yet another aspect, the present invention provides a method for preparing the fermented composition of traditional Chinese medicine, comprising:
(1) Extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water as extraction solvent under heating, filtering the extractive solution, and collecting supernatant to obtain water extractive solutions of radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba;
(2) Mixing the water extracts of radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with fermentation medium in a fermenter to obtain fermentation culture;
(3) And (3) respectively inoculating a saccharomycete strain and a lactobacillus strain into the fermentation culture for aerobic fermentation to obtain the microbial inoculum.
In the step (1), the dangshen, the angelica and the white paeony root are heated and boiled for extraction by using 8-12 times of water as an extraction solvent, and the supernatant is filtered, thus obtaining the Chinese medicinal composition.
As a preferred embodiment of the invention, the fermentation medium consists of a liquid common broth, caCO 3 、(NH 4 ) 2 SO 4 NaOH, soybean meal, corn and bran.
As a preferred embodiment of the invention, the weight parts of the components in the fermentation culture described in step (2) are preferably as follows: 60 parts of water extract of radix codonopsis pilosulae, angelica and white peony root, 30 parts of liquid common broth and CaCO 3 1.5 parts of (NH) 4 ) 2 SO 4 2 parts of NaOH 4 parts, 2 parts of bean pulp, 2 parts of corn and 2.5 parts of bran.
As a preferred embodiment of the present invention, in the step (3), after the pH value of the fermentation culture is adjusted to 7.0, the fermentation culture is inoculated with yeast and lactobacillus respectively for oxygen-consuming fermentation.
As a preferred embodiment of the present invention, in the step (3), 2% of yeast strain and 1.5% of lactobacillus strain are respectively inoculated into the fermentation culture for aerobic fermentation.
As a preferred embodiment of the present invention, the fermentation in step (3) is carried out at a temperature of 37 ℃.
As a preferred embodiment of the present invention, the conditions of the fermentation described in step (3) further comprise: the rotating speed of the motor of the fermentation tank is adjusted to 250r/min, and the ventilation is adjusted to 0.05m 3 /min。
In the traditional Chinese medicine composition, dangshen has the effects of strengthening the middle-jiao and replenishing qi, and strengthening the spleen and benefiting the lung. The dangshen contains dangshen acetylenic glycoside, polysaccharides, volatile oil, phenols, various amino acids necessary for human body, and the like, and has the effects of tonifying lung and stomach, regulating gastrointestinal movement, reducing ulcer, lowering blood pressure, resisting cancer and enhancing organism immunity. In addition, it is also resistant to adverse stress by eliciting a series of neuroendocrine responses that in turn counteract the excitatory effects of posterior pituitrin on the uterus. The Chinese angelica has the effects of enriching and nourishing blood, activating blood and removing stasis, and is the first essential medicine for enriching blood. The main active ingredients of radix Angelicae sinensis comprise ferulic acid, polysaccharide, flavone, etc., and have antibacterial, antiinflammatory, free radical scavenging, antioxidant, and immunity enhancing effects. The volatile oil in the angelica can reduce uterine rhythmic contraction, has a certain inhibiting effect on uterine contraction caused by pituitrin, acetylcholine and the like, and is beneficial to the growth and development of embryos. White peony root is called white peony root, and the 2015 edition of Chinese pharmacopoeia is summarized as blood nourishing, menstruation regulating, liver softening, pain relieving, liver yang suppressing, yin astringing and sweat suppressing. Modern pharmacological researches show that the white peony root contains paeoniflorin, paeonol, volatile oil, triterpenes and the like, has the effects of resisting inflammation, enhancing the body immunity regulating function, dilating blood vessels and the like, can obviously inhibit uterine contraction caused by acetylcholine, histamine and oxytocin, and can play a good role in regulating uterine and placenta functions.
The ferment bacteria of the invention preferably consists of saccharomycetes and lactobacillus, wherein the lactobacillus is typical anaerobic bacteria, the saccharomycetes are facultative anaerobic bacteria, when the saccharomycetes and the anaerobic bacteria are mixed for fermentation, the saccharomycetes firstly consume oxygen in the environment, provide proper fermentation conditions for the lactobacillus, and when the oxygen consumption of the saccharomycetes for fermentation reaches a certain degree, the lactobacillus starts to ferment and produce lactic acid. Meanwhile, saccharomycetes can provide necessary nutritional basis for the growth of lactobacillus. Therefore, the synergistic effect between the lactobacillus and the saccharomycetes makes the mixed fermentation effect obvious. The radix codonopsis pilosulae, the Chinese angelica and the white peony root are combined into the traditional Chinese medicine compound, so that the compound traditional Chinese medicine has the effects of tonifying middle-jiao and Qi, resisting bacteria and inflammation, resisting oxidation, enhancing organism immunity and the like, and the effective components of the compound traditional Chinese medicine compound can be fully exerted on preventing and treating diarrhea of piglets through fermentation.
The traditional Chinese medicine fermented feed additive provided by the invention has the advantages of improving the contents of traditional Chinese medicines, probiotics and active ingredients after fermentation, has the characteristics of good absorption, quick response, reduced toxic and side effects and the like, can prevent qi and blood deficiency of sows in gestation period after administration in the latter period of the sows, effectively improves the growth performance, oxidation resistance and disease resistance of piglets produced by the pregnant sows, has definite prevention effect on diarrhea of the piglets, and can effectively reduce the incidence rate of diarrhea of the piglets.
Therefore, the traditional Chinese medicine feed additive or the traditional Chinese medicine fermented feed additive provided by the invention has important application prospect in improving reproductive performance of pregnant sows, improving growth performance, oxidation resistance and disease resistance of piglets produced by the pregnant sows, and particularly in effectively reducing incidence of diarrhea of piglets produced by the pregnant sows.
For reference, when the traditional Chinese medicine feed additive or the traditional Chinese medicine fermented feed additive provided by the invention is applied, the traditional Chinese medicine feed additive or the traditional Chinese medicine fermented feed additive provided by the invention is added into the basic ration of pregnant sows, and preferably, the traditional Chinese medicine feed additive or the traditional Chinese medicine fermented feed additive provided by the invention is added into the basic ration of pregnant sows according to the adding proportion of 0.5 wt%.
Detailed Description
The present invention is further described below by way of specific examples, which are not intended to limit the scope of the invention. Modifications, combinations, or substitutions of the present invention within the scope of the invention or without departing from the spirit and scope of the invention will be apparent to those skilled in the art and are included within the scope of the invention.
Preparation of Chinese medicinal feed additive of preparation example 1
Cleaning radix Codonopsis, radix Angelicae sinensis, and radix Paeoniae alba, adding into an extraction tank, adding 10 times of water, boiling for 1 hr, and filtering; extracting the residue for 2 times; mixing the extractive solutions for 3 times to obtain water extractive solutions of radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba.
Preparation example 2 cultivation of Yeast species and Lactobacillus species
Yeast strain (purchased from Yunuo biology Co., dongguan) was inoculated with YPD medium and cultured in a constant temperature shaking incubator at a rotation speed of 200r/min and a temperature of 28℃for 24 hours. Lactobacillus strain (purchased from Yuno biology Co., dongguan) was inoculated into MRS medium and cultured in a constant temperature shaking incubator at a rotation speed of 180r/min at 37℃for 24 hours.
Example 1 preparation of Chinese herbal fermented feed additive
The preparation method comprises the following steps of:
60 parts of water extract of radix codonopsis pilosulae, angelica and white peony root (prepared in preparation example 1), 1.5 parts of lactobacillus, 2 parts of saccharomycetes, 30 parts of liquid common broth and CaCO 3 1.5 parts of (NH) 4 ) 2 SO 4 2 parts of NaOH 4 parts, 2 parts of bean pulp, 2 parts of corn and 2.5 parts of bran.
(II) preparation method:
(1) Extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water, broth, caCO 3 、(NH 4 ) 2 SO 4 Loading NaOH, bean pulp, corn and bran into a fermentation tank;
(2) Adding an anti-foam agent, and adjusting the initial pH value to 7.0;
(3) Inoculating yeast and lactobacillus respectively according to 2% and 1.5% of inoculum size;
(4) The rotating speed of the motor of the fermentation tank is adjusted to 250r/min, and the ventilation is adjusted to 0.05m 3 And/min, fermenting at 37deg.C for 48 hr.
Comparative example 1 preparation of Chinese medicinal fermented feed additive
The preparation method comprises the following steps of:
60% of aqueous extracts of radix codonopsis pilosulae, angelica and white peony root (prepared in preparation example 1), 2 parts of saccharomycetes, 2 parts of bacillus subtilis, 30 parts of liquid common broth and CaCO 3 1.5 parts of (NH) 4 ) 2 SO 4 2 parts of NaOH 4 parts, 2 parts of bean pulp, 2 parts of corn and 2.5 parts of bran.
(II) preparation method:
(1) Extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water, broth, caCO 3 、(NH 4 ) 2 SO 4 Loading NaOH, bean pulp, corn and bran into a fermentation tank;
(2) Adding an anti-foam agent, and adjusting the initial pH to 7.0;
(3) Inoculating the cultured yeast and bacillus subtilis strain (purchased from Yuno biology Co., dongguan) according to inoculum sizes of 2wt% and 1.5wt%, respectively;
(4) The rotating speed of the motor of the fermentation tank is adjusted to 250r/min, and the ventilation is adjusted to 0.05m 3 And/min, fermenting at 37deg.C for 48 hr.
Comparative example 2 preparation of Chinese medicinal fermented feed additive
The preparation method comprises the following steps of:
60 parts of water extract of radix codonopsis pilosulae, angelica and white peony root (prepared in preparation example 1), 1.5 parts of lactobacillus, 2 parts of saccharomycetes, 30 parts of liquid common broth and CaCO 3 1.5 parts of (NH) 4 ) 2 SO 4 2 parts of NaOH 4 parts, 2 parts of bean pulp, 2 parts of corn and 2.5 parts of bran.
(1) Extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water, broth, caCO 3 、(NH 4 ) 2 SO 4 Loading NaOH, bean pulp, corn and bran into a fermentation tank;
(2) Adding an anti-foam agent, and adjusting the initial pH to 7.0;
(3) Inoculating cultured enterococcus faecium (purchased from Yuno organism Co., dongguan) and lactobacillus strain (purchased from Yuno organism Co., dongguan) respectively in an inoculum size of 2wt% and 1.5 wt%;
(4) The rotating speed of the motor of the fermentation tank is adjusted to 250r/min, and the ventilation is adjusted to 0.05m 3 And/min, fermenting at 37deg.C for 48 hr.
Comparative example 3 preparation of Chinese medicinal fermented feed additive
The preparation method comprises the following steps of:
60 parts of water extract of radix codonopsis pilosulae, angelica and white peony root (prepared in preparation example 1), 1.5 parts of lactobacillus, 2 parts of saccharomycetes, 30 parts of liquid common broth and CaCO 3 1.5 parts of (NH) 4 ) 2 SO 4 2 parts of NaOH 4 parts, 2 parts of bean pulp, 2 parts of corn and 2.5 parts of bran.
(1) Extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water, broth, caCO 3 、(NH 4 ) 2 SO 4 Loading NaOH, bean pulp, corn and bran into a fermentation tank;
(2) Adding an anti-foam agent, and adjusting the initial pH to 7.0;
(3) Inoculating the cultured bacillus subtilis and rhizopus oryzae strains (purchased from Yuno biology Co., dongguan) according to the inoculum concentration of 2wt% and 1.5wt%, respectively;
(4) The rotating speed of the motor of the fermentation tank is adjusted to 250r/min, and the ventilation is adjusted to 0.05m 3 And/min, fermenting at 37deg.C for 48 hr.
Test example 1 influence test of Chinese medicinal fermented feed additive on sow reproductive performance and piglet growth performance
1. Test subjects
70 pregnant sows and piglets produced by the sows are randomly divided into four groups, namely, group C, group I, group II, group III, group IV, group V, group VI and 10 piglets per group.
2. Test method
Basic ration (corn soybean meal) feed for each group is as follows:
group C: feeding basic daily ration;
group I: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine preparation prepared in the preparation example 1 is added into basic ration, and feeding is carried out for 7 days;
group II: feeding mixed feed, wherein 0.5wt% of lactobacillus+saccharomycete prepared in preparation example 2 is added into basic ration, and feeding for 7 days;
group III: feeding mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the example 1 is added into basic ration, and feeding for 7 days;
group IV: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 1 is added into basic ration, and feeding is carried out for 7 days;
group V: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 2 is added into basic ration, and feeding is carried out for 7 days;
group VI: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 3 is added into basic ration, and feeding is carried out for 7 days;
the immune disinfection procedure and the feeding management are carried out according to the conventional method of pig farms. And the conditions of temperature, ventilation, illumination and the like of each group are kept consistent during the test period, and the test method can freely eat and drink water.
3. Test index detection
Recording total litter size, number of live births, weight of primary individuals and daily lactation capacity of each sow, and counting interval time from weaning to recurrent condition of the sows after weaning. The piglets are weighed at birth and 21 days of age, and the average birth weight, average weaning nest weight and average daily gain of the piglets are calculated.
4. Test results
As shown in table 1, the average weaning number per litter, average weight of weaning head, average daily gain, survival rate of 21-day-old piglets, daily lactation power of sows are all significantly improved (P < 0.05) compared with those of group C, the weaning litter weight of group III and the daily lactation power of sows are significantly improved (P < 0.01) compared with those of group C, and the recurrence interval time of group III sows is significantly reduced (P < 0.01) compared with those of group C. The average weaning number per litter, average weight of weaning heads, average daily gain, survival rate of 21-day-old piglets, weight of weaning litter, daily lactation capacity of sows are all superior to those of group I, group II and other fermentation groups (group IV, group V and group VI). The result shows that the traditional Chinese medicine fermented feed additive (group III) can promote the reproductive performance of sows and the growth of piglets.
Table 1 influence of a fermented feed additive in chinese medicine on reproductive performance of sows and growth performance of piglets
Note that: * The comparison difference between group III and group C, group I, group II, group IV, group V and group VI is significant (P < 0.05), the comparison difference between group III and group C, group I, group II, group IV, group V and group VI is extremely significant (P < 0.01), and the unlabeled difference is not significant (P > 0.05). Test example 2 test of influence of fermented feed additive on diarrhea and death of piglets
1. Test subjects
70 pregnant sows and piglets produced by the sows are randomly divided into four groups, namely, group C, group I, group II, group III, group IV, group V, group VI and 10 piglets per group.
2. Test method
Basic ration (corn soybean meal) feed for each group is as follows:
group C: feeding basic daily ration;
group I: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine preparation prepared in the preparation example 1 is added into basic ration, and feeding is carried out for 7 days;
group II: feeding mixed feed, wherein 0.5wt% of lactobacillus+saccharomycete prepared in preparation example 2 is added into basic ration, and feeding for 7 days;
group III: feeding mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the example 1 is added into basic ration, and feeding for 7 days;
group IV: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 1 is added into basic ration, and feeding is carried out for 7 days;
group V: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 2 is added into basic ration, and feeding is carried out for 7 days;
group VI: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 3 is added into basic ration, and feeding is carried out for 7 days;
the immune disinfection procedure and the feeding management are carried out according to the conventional method of pig farms. And the conditions of temperature, ventilation, illumination and the like of each group are kept consistent during the test period, and the test method can freely eat and drink water.
3. Test index detection
After sow farrowing, the fecal condition of the piglets is checked at early and late daily, and the diarrhea rate, diarrhea index and mortality of the piglets are calculated according to the following formula:
diarrhea index = sum of diarrhea scores per piglet/number of days of the piglet per piglet trial);
diarrhea rate (%) = number of diarrhea heads per piglet/(number of test days per piglet) x 100%;
mortality = number of dead piglets tested +.f. number of piglets tested x 100%.
4. Test results
The test results are shown in table 2, and by observing and recording the fecal and death conditions of piglets, the diarrhea index and death rate of the piglets in the group I-VI are both found to be significantly reduced compared with those in the group C (P < 0.05), wherein the diarrhea rate of the piglets in the group III is extremely significantly reduced compared with those in the group C (P < 0.01). Group III has a lower diarrhea index and mortality than group I, group II and other fermentation groups (group IV, group V, group VI). The result shows that in the traditional Chinese medicine fermented feed additive (group III), the lactobacillus and the microzyme are combined for fermentation, so that the effective components in the compound traditional Chinese medicine are fully released, new anti-inflammatory substances are possibly generated in the fermentation process, meanwhile, the lactobacillus and the microzyme can generate a large amount of organic acids and micromolecular active substances in the fermentation process, and the active substances can keep the balance of intestinal microecological environment, improve the digestion and absorption functions of the gastrointestinal tract, and further reduce diarrhea of piglets.
Table 2 Effect of traditional Chinese medicine fermented feed additive on diarrhea and death conditions of piglets
Note that: * The comparison difference between group III and group C, group I, group II, group IV, group V and group VI is significant (P < 0.05), the comparison difference between group III and group C, group I, group II, group IV, group V and group VI is extremely significant (P < 0.01), and the unlabeled difference is not significant (P > 0.05). Test example 3 influence test of Chinese medicinal fermented feed additive on serum Biochemical index of piglets
1. Test subjects
70 pregnant sows and piglets produced by the sows are randomly divided into four groups, namely, group C, group I, group II, group III, group IV, group V, group VI and 10 piglets per group.
2. Test method
Basic ration (corn soybean meal) feed for each group is as follows:
group C: feeding basic daily ration;
group I: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine preparation prepared in the preparation example 1 is added into basic ration, and feeding is carried out for 7 days;
group II: feeding mixed feed, wherein 0.5wt% of lactobacillus+saccharomycete prepared in preparation example 2 is added into basic ration, and feeding for 7 days;
group III: feeding mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the example 1 is added into basic ration, and feeding for 7 days;
group IV: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 1 is added into basic ration, and feeding is carried out for 7 days;
group V: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 2 is added into basic ration, and feeding is carried out for 7 days;
group VI: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 3 is added into basic ration, and feeding is carried out for 7 days;
the immune disinfection procedure and the feeding management are carried out according to the conventional method of pig farms. And the conditions of temperature, ventilation, illumination and the like of each group are kept consistent during the test period, and the test method can freely eat and drink water.
3. Test index detection
At 7d, 14d and 21d after the birth of the piglets, 5 piglets are randomly selected from the group C, the group I, the group II, the group III, the group IV and the group V, and 5mL of fasting anterior vena cava blood sampling is carried out on each group in the group VI. The blood is filled in a non-anticoagulation blood collection tube, centrifuged for 5min at 3000r/min of a centrifuge at 4 ℃, and the upper serum is removed and packaged and is preserved at-20 ℃ for testing.
And (3) taking the preserved piglet serum, and respectively measuring TP, GLB, GLU, ALP, ALB, BUN, TG, TC, AST and ALT by adopting a corresponding ELISA kit.
4. Test results
The test results are shown in table 3, and the levels of TP, GLB, GLU, ALP in the group III piglet serum were found to be significantly higher than those of group C (P < 0.05) by detecting the levels of biochemical indexes of the piglet serum, and the levels of TP, GLB, GLU, ALP in the group III piglet serum were all superior to those of group I, group II and other fermentation groups (group iv, group v, group vi). The results show that the traditional Chinese medicine fermented feed additive (group III) can enhance the absorption and utilization rate of proteins and the sugar metabolism capability of piglets, thereby enhancing the disease resistance of organisms.
TABLE 3 influence of traditional Chinese medicine fermented feed additives on serum Biochemical index of piglets
Note that: * The comparison difference between group III and group C, group I, group II, group IV, group V and group VI is significant (P < 0.05), the comparison difference between group III and group C, group I, group II, group IV, group V and group VI is extremely significant (P < 0.01), and the unlabeled difference is not significant (P > 0.05).
Test example 4 influence test of Chinese herbal fermented feed additive on piglet immune function
1. Test subjects
70 pregnant sows and piglets produced by the sows are randomly divided into four groups, namely, group C, group I, group II, group III, group IV, group V, group VI and 10 piglets per group.
2. Test method
Basic ration (corn soybean meal) feed for each group is as follows:
group C: feeding basic daily ration;
group I: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine preparation prepared in the preparation example 1 is added into basic ration, and feeding is carried out for 7 days;
group II: feeding mixed feed, wherein 0.5wt% of lactobacillus+saccharomycete prepared in preparation example 2 is added into basic ration, and feeding for 7 days;
group III: feeding mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the example 1 is added into basic ration, and feeding for 7 days;
group IV: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 1 is added into basic ration, and feeding is carried out for 7 days;
group V: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 2 is added into basic ration, and feeding is carried out for 7 days;
group VI: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 3 is added into basic ration, and feeding is carried out for 7 days;
the immune disinfection procedure and the feeding management are carried out according to the conventional method of pig farms. And the conditions of temperature, ventilation, illumination and the like of each group are kept consistent during the test period, and the test method can freely eat and drink water.
3. Test index detection
The 7d, 14d and 21d of the piglets after birth are selected from the group consisting of group C, group I, group II and group III,
And 5 piglets in each group of IV, V and VI are randomly selected for fasting anterior vena cava blood sampling by 5mL. The blood is filled in a non-anticoagulation blood collection tube, centrifuged for 5min at 3000r/min of a centrifuge at 4 ℃, and the upper serum is removed and packaged and is preserved at-20 ℃ for testing. Taking the preserved piglet serum, and respectively detecting the serum antibody IgG, igA, igM level by adopting a corresponding ELISA kit.
4. Test results
The test results are shown in table 4, and by detecting the content of the serum immunoglobulin of the piglets, the serum IgG of the piglets of the ages of 7 days and 21 days in group III and the serum IgA of the piglets of the ages of 14 days are obviously increased compared with the serum IgA of the piglets of the ages of C (P < 0.05), the serum IgG content of the piglets of the ages of 14 days in group III is extremely obviously increased compared with the serum IgM of the piglets of the ages of C (P < 0.01), and the serum IgA and IgM of the piglets of the ages of 7 days in group III and 21 days are also increased compared with the serum IgA and IgM of the piglets of the ages of 21 days in group C. The serum immunoglobulin content of the group III piglets is better than that of the group I, the group II and other fermentation groups (group IV, group V and group VI). The result shows that the traditional Chinese medicine fermented feed additive (group III) can obviously enhance the immunity of piglets.
TABLE 4 Effect of traditional Chinese medicine fermented feed additives on serum immunoglobulin content of piglets
Note that: * The comparison difference between group III and group C, group I, group II, group IV, group V and group VI is significant (P < 0.05), the comparison difference between group III and group C, group I, group II, group IV, group V and group VI is extremely significant (P < 0.01), and the unlabeled difference is not significant (P > 0.05).
Test example 5 influence test of traditional Chinese medicine fermented feed additive on antioxidant function of piglets
1. Test subjects
70 pregnant sows and piglets produced by the sows are randomly divided into four groups, namely, group C, group I, group II, group III, group IV, group V, group VI and 10 piglets per group.
2. Test method
Basic ration (corn soybean meal) feed for each group is as follows:
group C: feeding basic daily ration;
group I: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine preparation prepared in the preparation example 1 is added into basic ration, and feeding is carried out for 7 days;
group II: feeding mixed feed, wherein 0.5wt% of lactobacillus+saccharomycete prepared in preparation example 2 is added into basic ration, and feeding for 7 days;
group III: feeding mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the example 1 is added into basic ration, and feeding for 7 days;
group IV: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 1 is added into basic ration, and feeding is carried out for 7 days;
group V: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 2 is added into basic ration, and feeding is carried out for 7 days;
group VI: feeding a mixed feed, wherein 0.5wt% of the traditional Chinese medicine fermented feed additive prepared in the comparative example 3 is added into basic ration, and feeding is carried out for 7 days;
the immune disinfection procedure and the feeding management are carried out according to the conventional method of pig farms. And the conditions of temperature, ventilation, illumination and the like of each group are kept consistent during the test period, and the test method can freely eat and drink water.
3. Test index detection
From group C, group I, group II, group III at 7d, 14d, 21d after birth of the piglets,
and 5 piglets in each group of IV, V and VI are randomly selected for fasting anterior vena cava blood sampling by 5mL. The blood is filled in a non-anticoagulation blood collection tube, centrifuged for 5min at 3000r/min of a centrifuge at 4 ℃, and the upper serum is removed and packaged and is preserved at-20 ℃ for testing. Taking the preserved piglet serum, and respectively detecting the levels of GAT, T-AOC, T-SOD, GSH-PX, MDA, GSH and GSSG by adopting corresponding ELISA kits.
4. Test results
By detecting the level of the serum antioxidant index of the piglets, the result shows that: CAT, T-AOC, T-SOD in group III sera of 7, 14, 21 piglets were significantly higher than those in group C (P < 0.05), MDA content in group III sera of 7, 14, 21 piglets was significantly lower than that in group C (P < 0.05). The index of group III is better than that of group I, group II and other fermentation groups (group IV, group V and group VI). The result shows that the traditional Chinese medicine fermented feed additive (group III) can obviously enhance the oxidation resistance of piglets.
TABLE 5 influence of traditional Chinese medicine fermented feed additives on serum antioxidant index level of piglets
Note that: * The comparison difference between group III and group C, group I, group II, group IV, group V and group VI is significant (P < 0.05), the comparison difference between group III and group C, group I, group II, group IV, group V and group VI is extremely significant (P < 0.01), and the unlabeled difference is not significant (P > 0.05).
Claims (10)
1. A traditional Chinese medicine feed additive for feeding pregnant sows is characterized by comprising radix codonopsis pilosulae, chinese angelica and white peony root.
2. The traditional Chinese medicine feed additive according to claim 1, wherein the traditional Chinese medicine feed additive comprises the following components in parts by weight: 0.5-4 parts of radix codonopsis pilosulae, 0.5-4 parts of angelica, and 0.5-4 parts of white peony root; preferably, the weight parts of the components are as follows: 1 part of radix codonopsis pilosulae, 1 part of angelica, and 1 part of white peony root.
3. A traditional Chinese medicine fermented feed additive for feeding pregnant sows, which is characterized by comprising: a traditional Chinese medicine composition, a fermentation microbial inoculum and a fermentation medium; the traditional Chinese medicine composition consists of dangshen, angelica and white paeony root, and the zymophyte agent consists of saccharomycetes and lactobacillus.
4. The fermented feed additive of claim 3, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 0.5-4 parts of radix codonopsis pilosulae, 0.5-4 parts of angelica, and 0.5-4 parts of white peony root; preferably, the weight parts of the components are as follows: 1 part of radix codonopsis pilosulae, 1 part of angelica, and 1 part of white peony root.
5. A method of preparing the traditional Chinese medicine feed additive of claim 1 or 2, comprising: extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water as extraction solvent under heating, filtering the extractive solution, and collecting supernatant; preferably, the radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba are extracted with 8-12 times of water as extraction solvent by heating and boiling, filtering, and collecting supernatant.
6. A method of preparing the traditional Chinese medicine fermented feed additive of claim 3 or 4, comprising:
(1) Extracting radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with water as extraction solvent under heating, filtering the extractive solution, and collecting supernatant to obtain water extractive solutions of radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba;
(2) Mixing the water extracts of radix Codonopsis, radix Angelicae sinensis and radix Paeoniae alba with fermentation medium in a fermenter to obtain fermentation culture;
(3) And (3) respectively inoculating a saccharomycete strain and a lactobacillus strain into the fermentation culture for aerobic fermentation to obtain the microbial inoculum.
7. The method according to claim 6, wherein in the step (1), the dangshen, the angelica and the white peony root are extracted by heating and boiling with 8-12 times of water as an extraction solvent, and the supernatant is obtained after filtration;
the fermentation medium in the step (2) is prepared from liquid common broth, caCO 3 、(NH 4 ) 2 SO 4 NaOH, soybean meal, corn and bran;
the fermentation culture in the step (2) comprises the following components in parts by weight: 60 parts of water extract of radix codonopsis pilosulae, angelica and white peony root, 30 parts of liquid common broth and CaCO 3 1.5 parts by weight、(NH 4 ) 2 SO 4 2 parts of NaOH 4 parts, 2 parts of bean pulp, 2 parts of corn and 2.5 parts of bran;
step (3), after the pH value of the fermentation culture is regulated to 7.0, respectively inoculating saccharomycetes and lactobacillus for oxygen consumption fermentation;
in the step (3), 2% of saccharomycete strain and 1.5% of lactobacillus strain are respectively inoculated into the fermentation culture for aerobic fermentation;
the fermentation temperature in the step (3) is 37 ℃;
the conditions of the fermentation described in step (3) further include: the rotating speed of the motor of the fermentation tank is adjusted to 250r/min, and the ventilation is adjusted to 0.05m 3 /min。
8. Use of the traditional Chinese medicine feed additive according to claim 1 or 2, or the traditional Chinese medicine fermented feed additive according to claim 3 or 4 for preparing a medicine or feed additive for promoting reproductive performance of pregnant sows.
9. Use of the traditional Chinese medicine feed additive according to claim 1 or 2, or the traditional Chinese medicine fermented feed additive according to claim 3 or 4 for preparing a medicine or feed additive for improving the immune function and oxidation resistance of piglets produced by pregnant sows.
10. Use of the traditional Chinese medicine feed additive according to claim 1 or 2, or the traditional Chinese medicine fermented feed additive according to claim 3 or 4 for preparing a medicament or feed additive for improving diarrhea resistance of piglets produced by pregnant sows.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104171500A (en) * | 2014-07-02 | 2014-12-03 | 中泰和（北京）科技发展有限公司 | Sow feed additive capable of improving immunity of piglets and using method thereof |
| CN106666161A (en) * | 2017-01-06 | 2017-05-17 | 北京中农弘科生物技术有限公司 | Application of yeast culture in preparation of feed capable of improving reproductive performance and relieving constipation of sows |
| US20180289038A1 (en) * | 2017-04-11 | 2018-10-11 | Church & Dwight Co., Inc. | Yeast And Bacterial Probiotics Combinations And Methods Of Use To Improve Swine Production |
| AU2020100242A4 (en) * | 2019-02-22 | 2020-04-02 | Chengdu Fenglan Technology Co.,Ltd | Wet-base fermented feed for perinatal sow and preparation method thereof |
| AU2020103400A4 (en) * | 2020-11-11 | 2021-01-28 | Nanjing Forestry University | A fermented plant feed additive for weaned piglet |
| CN113317408A (en) * | 2021-06-21 | 2021-08-31 | 河北农业大学 | Feed additive capable of remarkably improving production performance of pregnant sows and pigsty environment and using method thereof |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104171500A (en) * | 2014-07-02 | 2014-12-03 | 中泰和（北京）科技发展有限公司 | Sow feed additive capable of improving immunity of piglets and using method thereof |
| CN106666161A (en) * | 2017-01-06 | 2017-05-17 | 北京中农弘科生物技术有限公司 | Application of yeast culture in preparation of feed capable of improving reproductive performance and relieving constipation of sows |
| US20180289038A1 (en) * | 2017-04-11 | 2018-10-11 | Church & Dwight Co., Inc. | Yeast And Bacterial Probiotics Combinations And Methods Of Use To Improve Swine Production |
| AU2020100242A4 (en) * | 2019-02-22 | 2020-04-02 | Chengdu Fenglan Technology Co.,Ltd | Wet-base fermented feed for perinatal sow and preparation method thereof |
| AU2020103400A4 (en) * | 2020-11-11 | 2021-01-28 | Nanjing Forestry University | A fermented plant feed additive for weaned piglet |
| CN113317408A (en) * | 2021-06-21 | 2021-08-31 | 河北农业大学 | Feed additive capable of remarkably improving production performance of pregnant sows and pigsty environment and using method thereof |
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