CN116195510A - Breeding method of novel variety of wheat with high photosynthetic rate - Google Patents
Breeding method of novel variety of wheat with high photosynthetic rate Download PDFInfo
- Publication number
- CN116195510A CN116195510A CN202310175017.XA CN202310175017A CN116195510A CN 116195510 A CN116195510 A CN 116195510A CN 202310175017 A CN202310175017 A CN 202310175017A CN 116195510 A CN116195510 A CN 116195510A
- Authority
- CN
- China
- Prior art keywords
- generation
- single plants
- wheat
- spad
- photosynthetic rate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding new varieties of wheat with high photosynthetic rate, which belongs to the technical field of wheat breeding, wherein parents, F1, F2, F3, F4 and F5 generations are bred through gas exchange parameters, chlorophyll fluorescence parameters and chlorophyll SPAD values, then F6 generation seeds obtained by planting the bred F5 generation are used as good varieties, selection indexes and numerical values are set according to specific conditions of the parents and different generation materials, and finally, accurate and efficient selection of the wheat Gao Guangge rate can be realized, so that technical support is provided for breeding new varieties of wheat with high photosynthetic rate.
Description
Technical Field
The invention relates to the technical field of wheat variety breeding, in particular to a novel wheat variety breeding method with high photosynthetic rate.
Background
Plant type breeding in the last 60 th century effectively reduces the plant height of wheat, improves plant type structure, enhances lodging resistance, remarkably improves population density and leaf area index, and particularly improves harvest index from 30% -35% in the early stage of construction to about 45% -50% at present, and 40% -50%. At present, the wheat plant type structure tends to be reasonable, the harvest index is difficult to be greatly improved, and the space for improving the yield level through morphological improvement is limited. Therefore, on the premise of keeping the existing wheat harvesting index level, improving the biological yield of the wheat is a key for realizing the great improvement of the wheat yield level again. A great deal of research shows that photosynthesis is a source of plant assimilation products, and the improvement of plant photosynthetic efficiency is a key for improving the biological yield of the plants, so that the improvement of the photosynthetic efficiency of wheat is a main technical approach for further improving the yield of the wheat.
The traditional method for selecting the new variety of the wheat with high photosynthetic rate generally carries out offspring selection according to a pedigree method, breeders do not generally carry out photosynthesis related parameter measurement on parents and offspring, after obtaining a strain with the characteristics of over F6 generation being orderly and consistent, whether the material has the characteristic of high photosynthetic rate is often judged according to the green holding property, biomass and yield of the material leaves, and the selection method has certain blindness and is difficult to accurately select the Gao Guangge rate characteristic of the wheat.
Therefore, how to provide a precise directional breeding method of a new variety of wheat with high photosynthetic rate is a problem to be solved by the technicians in the field.
Disclosure of Invention
In view of the above, the invention provides a method for breeding new wheat varieties with high photosynthetic rate, which integrates a gas exchange parameter measurement technology, a chlorophyll fluorescence parameter measurement technology and a chlorophyll relative content rapid measurement technology, and sets selection indexes and numerical values according to specific conditions of parents and different generation materials, so that accurate and efficient selection of the Gao Guangge rate of wheat can be finally realized, and technical support is provided for breeding new wheat varieties with high photosynthetic rate resistance.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for breeding new varieties of wheat with high photosynthetic rate comprises the following steps:
1) Hybridization is carried out by taking Zhengmai 7698 as a female parent and medium breeding 1026 as a male parent, thus obtaining F1 generation;
2) Sowing F1 generation seeds to obtain F2 seeds, and obtaining F2 generation plants after planting;
3) F2 generation single plants with excellent comprehensive disease resistance, high yield and stress resistance are selected, PSII maximum photochemical efficiency Fv/Fm of the single plants is measured 20 days after flowering, single plants with Fv/Fm more than or equal to 0.78 are selected, SPAD values of the selected single plants are measured, and single plants with SPAD more than or equal to 48.0 are selected, so that F2 generation single plants meeting index requirements are finally obtained;
4) Sowing F3 generation seeds, selecting F3 generation single plants with excellent comprehensive disease resistance, high yield and stress resistance, measuring PSII maximum photochemical efficiency Fv/Fm of the single plants 20 days after flowering, selecting single plants with Fv/Fm being more than or equal to 0.8, simultaneously measuring SPAD values of the selected single plants, selecting single plants with SPAD being more than or equal to 50.0, and finally obtaining F3 generation single plants meeting index requirements;
5) Sowing F4 generation seeds, and obtaining excellent F5 generation strains by the screening method in the same step 4);
6) F5 generation strain with excellent properties is bred, photosynthetic rate and SPAD value are measured in flowering period and 20 days after flowering, and photosynthetic rate of the flowering period is selected to be more than or equal to 26.0 mu molCO 2 .m -2 .s -1 SPAD value is 55.0, 20 days after flowering, the photosynthetic rate is more than or equal to 24.0 mu molCO 2 .m -2 .s -1 And (3) harvesting F6 generation seeds, namely a new wheat variety with high photosynthetic rate, from an excellent single plant with a SPAD value of 50.0.
In summary, compared with the prior art, the novel variety breeding technology of the high photosynthetic rate wheat provided by the invention can accurately breed the novel variety of the high photosynthetic rate wheat, and the physiological index rapid measurement technology such as the gas exchange parameter, the chlorophyll fluorescence parameter, the chlorophyll SPAD value and the like are integrated from the parent selection to the selection process of the F1, F2, F3, F4, F5 and F6 generation offspring, so that the accuracy of character selection is greatly improved.
The invention also sets different measurement indexes and index values according to the group characteristics of different generations, for example: considering that the F2, F3 and F4 generation materials have large groups, the combined chlorophyll fluorescence and chlorophyll SPAD value measurement technology has the characteristic of high-throughput rapid measurement, so that the measurement of chlorophyll fluorescence parameters Fv/Fm and SPAD values is only carried out in the three generations, and the measurement is only carried out in the key growth period of 20 days after flowering. On the other hand, to prevent the selection of excellent individuals, the selection criteria for Fv/Fm and SPAD values were also suitably reduced to 0.78 and 48.0 in the F2 generation to ensure that excellent individuals could be selected into the next generation.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
(1) Parent selection and single cross combination configuration.
Screening parent materials of high photosynthetic rate of Zhengmai 7698 and Zhongyu 1026, the net photosynthetic rate of flag leaves of the two materials is 26.2 mu molCO, and the flowering period is 26 mu molCO 2 ·m -2 ·s -1 And 27.3. Mu. MolCO 2 ·m -2 ·s -1 25.5 mu molCO each 20 days after flowering 2 ·m -2 ·s -1 And 24.8. Mu. MolCO 2 ·m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Flag leaf SPAD values, flowering period 55.5 and 55.6, respectively, and 20 days after flowering 52.4 and 51.5, respectively. Hybrid combination is configured by taking Zhengmai 7698 as a female parent and Zhongyu 1026 as a male parent.
(2) F2 generation material selection method.
And mixing the harvested F1 generation single plant seeds together, selecting 2000 seeds for sowing, wherein the row spacing is 26cm, the plant spacing is 10cm, and the row length is 1800cm, and finally obtaining 1790 plants of F2 offspring group. Firstly, selecting 130 single plants with excellent comprehensive disease resistance, high yield, stress resistance and other agronomic characters. On the basis, the maximum photochemical efficiency F of PSII of the selected individual strain is measured by using a saturated pulse type fluorometer 20 days after wheat flowers v /F m Select F v /F m The single plant with the length of more than or equal to 0.78 is measured by utilizing a SPAD chlorophyll meter to measure the SPAD value of the selected single plant, and the single plant with the length of more than or equal to 48.0 is selected to finally obtain 60 single plants meeting the index requirement。
(5) And F3 generation material selection method.
And (3) eliminating 8F 1 single plants by indoor seed examination, and finally sowing 52F 2 single plant seeds selected in the middle into F3 generation seeds according to the plant line, wherein the seeds of each F2 single plant are planted into 2 double-row areas, the wide and narrow row planting is performed, the wide row width is 27cm, the narrow row width is 18cm, the plant spacing is 6.6cm, and the row length is 200cm. First, selecting 122 single plants with excellent agronomic characters. Based on the above, the maximum photochemical efficiency F of PSII of the selected individual is measured by a saturated pulse type fluorometer v /F m Select F v /F m And (3) measuring the SPAD value of the selected single plant by utilizing a SPAD chlorophyll meter, and selecting the single plant with the SPAD of more than or equal to 50.0 to finally obtain 51 single plants (4F 3 generation strains) meeting the index requirements.
(6) And F4 generation material selection method.
Sowing the harvested seeds of 51F 3 single plants into F4 generation according to the plant lines, wherein the total number of the seeds is 4, the seeds are planted in large lines, wide and narrow lines, the width of the wide lines is 27cm, the width of the narrow lines is 18cm, the plant spacing is 6.6cm, the line length is 200cm, and each F3 single plant seed is planted into 1 double-line area. The field observation shows that the offspring materials are still in a separated state, and a single 110 strain with excellent comprehensive agronomic performance is selected. Based on the above, the maximum photochemical efficiency F of PSII of the selected individual is measured by a saturated pulse type fluorometer v /F m Select F v /F m And (3) measuring SPAD values of selected single plants by utilizing a SPAD chlorophyll meter, and selecting single plants with SPAD of more than or equal to 50.0 to finally obtain 42 single plants, 3F 4 generation strains and 164-3-10, 164-15-3 and 164-36-19 strain codes meeting the index requirements.
(7) And F5 generation material selection method.
Selecting single plants from the 3 harvested strains and 42 plants, sowing the single plants into F5 generation according to the strain, planting the single plants in wide and narrow rows, wherein the width of the wide rows is 27cm, the width of the narrow rows is 18cm, the plant spacing is 6.6cm, the row length is 200cm, and planting seeds of each F4 single plant into 2 double-row areas. The field observation shows that the agronomic characters are basically consistent, and 20 strains with excellent agronomic character and orderly consistent characters are selected. On the basis, 5 representative strains are selected from the 20 strains respectively, and the representative strains are respectively in flowering period and flowering periodMeasuring photosynthetic rate and SPAD value 20 days after flowers, and selecting flowering stage photosynthetic rate not less than 26.0 mu molCO 2 ·m -2 ·s -1 SPAD value is 55.0, 20 days after flowering, the photosynthetic rate is more than or equal to 24.0 mu molCO 2 ·m -2 ·s -1 The excellent single plants with SPAD value of 50.0 finally obtain 4 plants with excellent comprehensive agronomic characters and meeting the index requirements, which are 164-3-10-8, 164-15-3-11, 164-15-3-15 and 164-36-19-2 respectively.
Example 2 verification of feasibility of example methods
Sowing the harvested 4 excellent strain seeds into 6 line area cells with the line spacing of 23.1cm and the line length of 4.26m, repeating 225 ten thousand/ha basic seedlings for 3 times, selecting 5 representative sample strains in each cell, and measuring the photosynthetic rate and SPAD value of flag leaves 20 days after flowering, wherein the result shows that: 164-3-10-8,
The photosynthetic rates of 164-15-3-11, 164-15-3-15, 164-36-19-2 at 20 days after flowering were 25.5. Mu. MolCO, respectively 2 ·m -2 ·s -1 、26.0μmolCO 2 ·m -2 ·s -1 、25.8μmolCO 2 ·m -2 ·s -1 、
25.6μmolCO 2 ·m -2 ·s -1 SPAD values are respectively 51.3, 52.0, 51.7 and 52.1, and the yields respectively reach 9109.5kg/ha, 8920.5kg/ha, 9120.0kg/ha and 9307.5kg/ha, and the yields are respectively increased by 4.29%, 2.13%, 4.41% and 6.56% compared with the regional test control Bainong 207. 164-36-19-2, the yield increasing ratio exceeds 6%, various selection indexes are achieved, and the final selected strain is named as Zhengmai 1936.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (1)
1. A method for breeding new varieties of wheat with high photosynthetic rate is characterized in that the process comprises the following steps:
1) Hybridization is carried out by taking Zhengmai 7698 as a female parent and medium breeding 1026 as a male parent, thus obtaining F1 generation;
2) Sowing F1 generation seeds to obtain F2 seeds, and obtaining F2 generation plants after planting;
3) F2 generation single plants with excellent comprehensive disease resistance, high yield and stress resistance are selected, PSII maximum photochemical efficiency Fv/Fm of the single plants is measured 20 days after flowering, single plants with Fv/Fm more than or equal to 0.78 are selected, SPAD values of the selected single plants are measured, and single plants with SPAD more than or equal to 48.0 are selected, so that F2 generation single plants meeting index requirements are finally obtained;
4) Sowing F3 generation seeds, selecting F3 generation single plants with excellent comprehensive disease resistance, high yield and stress resistance, measuring PSII maximum photochemical efficiency Fv/Fm of the single plants 20 days after flowering, selecting single plants with Fv/Fm being more than or equal to 0.8, simultaneously measuring SPAD values of the selected single plants, selecting single plants with SPAD being more than or equal to 50.0, and finally obtaining F3 generation single plants meeting index requirements;
5) Sowing F4 generation seeds, and obtaining excellent F5 generation strains by the screening method in the same step 4);
6) F5 generation strain with excellent properties is bred, photosynthetic rate and SPAD value are measured in flowering period and 20 days after flowering, and photosynthetic rate of the flowering period is selected to be more than or equal to 26.0 mu molCO 2 .m -2 .s -1 SPAD value is 55.0, 20 days after flowering, the photosynthetic rate is more than or equal to 24.0 mu molCO 2 .m -2 .s -1 And (3) harvesting F6 generation seeds, namely a new wheat variety with high photosynthetic rate, from an excellent single plant with a SPAD value of 50.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310175017.XA CN116195510A (en) | 2023-02-28 | 2023-02-28 | Breeding method of novel variety of wheat with high photosynthetic rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310175017.XA CN116195510A (en) | 2023-02-28 | 2023-02-28 | Breeding method of novel variety of wheat with high photosynthetic rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116195510A true CN116195510A (en) | 2023-06-02 |
Family
ID=86512502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310175017.XA Pending CN116195510A (en) | 2023-02-28 | 2023-02-28 | Breeding method of novel variety of wheat with high photosynthetic rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116195510A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115943887A (en) * | 2023-02-28 | 2023-04-11 | 河南省作物分子育种研究院 | Breeding method of new variety of wheat with strong light resistance and high temperature resistance |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857229A (en) * | 2017-02-15 | 2017-06-20 | 山东省农业科学院农产品研究所 | A kind of Application way of Britain's wheat germplasm |
| CN107356569A (en) * | 2017-06-06 | 2017-11-17 | 河南农业大学 | The construction method of method and its model based on chlorophyll fluorescence prediction wheat grain yield |
| CN109479709A (en) * | 2018-10-30 | 2019-03-19 | 陕西省杂交油菜研究中心 | A kind of screening technique of high photosynthetic efficiency polyploid wheat |
| CN115943887A (en) * | 2023-02-28 | 2023-04-11 | 河南省作物分子育种研究院 | Breeding method of new variety of wheat with strong light resistance and high temperature resistance |
-
2023
- 2023-02-28 CN CN202310175017.XA patent/CN116195510A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106857229A (en) * | 2017-02-15 | 2017-06-20 | 山东省农业科学院农产品研究所 | A kind of Application way of Britain's wheat germplasm |
| CN107356569A (en) * | 2017-06-06 | 2017-11-17 | 河南农业大学 | The construction method of method and its model based on chlorophyll fluorescence prediction wheat grain yield |
| CN109479709A (en) * | 2018-10-30 | 2019-03-19 | 陕西省杂交油菜研究中心 | A kind of screening technique of high photosynthetic efficiency polyploid wheat |
| CN115943887A (en) * | 2023-02-28 | 2023-04-11 | 河南省作物分子育种研究院 | Breeding method of new variety of wheat with strong light resistance and high temperature resistance |
Non-Patent Citations (4)
| Title |
|---|
| 张雯婷等: "小偃54和8602及其杂交后代小偃41和小偃81花后旗叶光合特性比较", 《作物学报》, vol. 37, no. 11, pages 2053 * |
| 江华等: "小麦杂交后代的光合作用", 《作物学报》, vol. 28, no. 4, pages 1 - 1 * |
| 王翠翠等: "小麦亲本及子代光合特性研究", 《山东植物生理学会第七次代表大会暨植物生物学与现代农业研讨会论文集》, pages 150 * |
| 金立桥等: "高产小麦品种郑麦7698花后光合特性研究", 《河南农业科学》, vol. 48, no. 1, pages 17 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115943887A (en) * | 2023-02-28 | 2023-04-11 | 河南省作物分子育种研究院 | Breeding method of new variety of wheat with strong light resistance and high temperature resistance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5530099B2 (en) | Methods for constructing and producing collective varieties in crop plants | |
| CN100493341C (en) | Method for selecting wheat of new variety | |
| CN104025998B (en) | Utilize the method for hybridized induction monoploid selecting and breeding corn inbred line | |
| CN105010129A (en) | Precocity upland cotton new-germplasm breeding method | |
| CN116195510A (en) | Breeding method of novel variety of wheat with high photosynthetic rate | |
| CN110100672B (en) | Method for rapidly producing rice photo-thermo-sensitive sterile line stock | |
| CN115943887A (en) | Breeding method of new variety of wheat with strong light resistance and high temperature resistance | |
| CN101019507A (en) | Breeding process of high temperature resisting cotton hybrid | |
| CN104813926A (en) | Breeding method capable of resisting wheat yellow mosaic disease | |
| CN111357654A (en) | Breeding method of new early-maturing high-sugar variety suitable for mechanized sugarcane | |
| CN103430828A (en) | Breeding method for improving high and stable yield of rice | |
| CN115633635A (en) | Method for creating sweet corn DH line based on haploid breeding technology | |
| CN115777526A (en) | Breeding method of fine grain fragrance type high-quality temperature-sensitive rice genic male sterile line | |
| CN105638448B (en) | It is a kind of to select the clonal method of excellent Chinese fir by characteristic index in the phase of cutting down | |
| CN110352847B (en) | Rapid breeding and combined testing and matching identification method for cotton nuclear male sterile line | |
| CN107667852B (en) | Production method and application of rice seeds | |
| CN112655546A (en) | High-yield directional improvement method for three capsule type sesame | |
| CN105993924A (en) | Breeding method of close-planting-resistant high-yield cabbage type rape hybrid varieties and application of obtained rape hybrid varieties | |
| CN107278875B (en) | Method for breeding new spring soybean variety by using soybean male nuclear sterile material | |
| CN105918107A (en) | Breeding method for repairing and improving natural doubling rate of maize haploid male flower by utilizing recurrent selection | |
| CN111149691A (en) | Method for breeding weak light-resistant inbred line by utilizing tomato hybrid | |
| CN1225963C (en) | Method for breeding new breed of pear of self-pollination and bear fruit | |
| CN115104528B (en) | Method for improving rice blast resistance | |
| CN111990248B (en) | Breeding method for cultivating novel ornamental dwarf sugarcane germplasm | |
| CN1119927C (en) | Breeding method for high-yield, disease-resistant, high-quality rice sterility series and its hybrid combination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |