CN116114515A - Method for controlling thrips pests by utilizing combination of trapping plants, pesticides and repellents - Google Patents
Method for controlling thrips pests by utilizing combination of trapping plants, pesticides and repellents Download PDFInfo
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- CN116114515A CN116114515A CN202310034097.7A CN202310034097A CN116114515A CN 116114515 A CN116114515 A CN 116114515A CN 202310034097 A CN202310034097 A CN 202310034097A CN 116114515 A CN116114515 A CN 116114515A
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- 241001414989 Thysanoptera Species 0.000 title claims abstract description 55
- 239000005871 repellent Substances 0.000 title claims abstract description 53
- 230000002940 repellent Effects 0.000 title claims abstract description 53
- 230000005532 trapping Effects 0.000 title claims abstract description 38
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000000575 pesticide Substances 0.000 title claims abstract description 14
- HFPZCAJZSCWRBC-UHFFFAOYSA-N p-cymene Chemical compound CC(C)C1=CC=C(C)C=C1 HFPZCAJZSCWRBC-UHFFFAOYSA-N 0.000 claims abstract description 20
- PSQYTAPXSHCGMF-BQYQJAHWSA-N β-ionone Chemical compound CC(=O)\C=C\C1=C(C)CCCC1(C)C PSQYTAPXSHCGMF-BQYQJAHWSA-N 0.000 claims abstract description 20
- YHQGMYUVUMAZJR-UHFFFAOYSA-N α-terpinene Chemical compound CC(C)C1=CC=C(C)CC1 YHQGMYUVUMAZJR-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 11
- SFEOKXHPFMOVRM-UHFFFAOYSA-N (+)-(S)-gamma-ionone Natural products CC(=O)C=CC1C(=C)CCCC1(C)C SFEOKXHPFMOVRM-UHFFFAOYSA-N 0.000 claims abstract description 10
- CUKAXHVLXKIPKF-ARJAWSKDSA-N (Z)-4-Hepten-1-ol Chemical compound CC\C=C/CCCO CUKAXHVLXKIPKF-ARJAWSKDSA-N 0.000 claims abstract description 10
- WSTYNZDAOAEEKG-UHFFFAOYSA-N Mayol Natural products CC1=C(O)C(=O)C=C2C(CCC3(C4CC(C(CC4(CCC33C)C)=O)C)C)(C)C3=CC=C21 WSTYNZDAOAEEKG-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002917 insecticide Substances 0.000 claims abstract description 7
- 238000005507 spraying Methods 0.000 claims abstract description 4
- 229940057995 liquid paraffin Drugs 0.000 claims description 17
- 239000002904 solvent Substances 0.000 claims description 7
- SPSPIUSUWPLVKD-UHFFFAOYSA-N 2,3-dibutyl-6-methylphenol Chemical compound CCCCC1=CC=C(C)C(O)=C1CCCC SPSPIUSUWPLVKD-UHFFFAOYSA-N 0.000 claims description 5
- 235000010354 butylated hydroxytoluene Nutrition 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 4
- CXEGAUYXQAKHKJ-NSBHKLITSA-N emamectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](NC)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 CXEGAUYXQAKHKJ-NSBHKLITSA-N 0.000 claims description 3
- 239000005889 Cyantraniliprole Substances 0.000 claims description 2
- DVBUIBGJRQBEDP-UHFFFAOYSA-N cyantraniliprole Chemical group CNC(=O)C1=CC(C#N)=CC(C)=C1NC(=O)C1=CC(Br)=NN1C1=NC=CC=C1Cl DVBUIBGJRQBEDP-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 15
- 230000002265 prevention Effects 0.000 abstract description 6
- XOBOCRSRGDBOGH-UHFFFAOYSA-N 5-phenylnonan-5-ol Chemical compound CCCCC(O)(CCCC)C1=CC=CC=C1 XOBOCRSRGDBOGH-UHFFFAOYSA-N 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 230000009471 action Effects 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 241000894007 species Species 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 33
- 241000196324 Embryophyta Species 0.000 description 21
- 241000238631 Hexapoda Species 0.000 description 15
- 238000002360 preparation method Methods 0.000 description 10
- 230000004044 response Effects 0.000 description 9
- 241000927584 Frankliniella occidentalis Species 0.000 description 8
- 241000220223 Fragaria Species 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000365769 Thrips flavus Species 0.000 description 6
- 238000009472 formulation Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000012086 standard solution Substances 0.000 description 5
- 240000001519 Verbena officinalis Species 0.000 description 4
- 230000000638 stimulation Effects 0.000 description 4
- 235000021012 strawberries Nutrition 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 235000016623 Fragaria vesca Nutrition 0.000 description 3
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000018718 Verbena officinalis Nutrition 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000001846 repelling effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000555682 Forsythia x intermedia Species 0.000 description 1
- 241000935799 Lefroyothrips lefroyi Species 0.000 description 1
- 241001477931 Mythimna unipuncta Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000339373 Thrips palmi Species 0.000 description 1
- ABUGVBRDFWGJRD-CHOYNLESSA-N [9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-2-(2,4-dinitrophenyl)sulfanylpurin-6-yl] [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C2=NC(SC=3C(=CC(=CC=3)[N+]([O-])=O)[N+]([O-])=O)=NC(OP(O)(=O)OP(O)(=O)OP(O)(O)=O)=C2N=C1 ABUGVBRDFWGJRD-CHOYNLESSA-N 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M29/00—Scaring or repelling devices, e.g. bird-scaring apparatus
- A01M29/12—Scaring or repelling devices, e.g. bird-scaring apparatus using odoriferous substances, e.g. aromas, pheromones or chemical agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P17/00—Pest repellants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Insects & Arthropods (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Birds (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for preventing and controlling thrips pests by combining trapping plants, pesticides and repellents, which is characterized in that the repellents are hung on the upper part of protected crops, one row of plants capable of trapping thrips are planted on two sides of the protected crops, and then the trapping plants are sprayed with the pesticides, so that the trapped thrips are thoroughly killed, wherein the repellents are prepared by mixing beta-ionone, cis-4-heptenol, alpha-terpinene, 4-isopropyl toluene and dibutyl hydroxyl toluene with the same mass according to 100 mug/ml. The repellent used in the invention is a plant source substance, the species is various, the action target site is wide, the used trapping plants have a strong trapping effect on thrips pests, and the thrips can be thoroughly killed by utilizing the toxicity of the insecticide by spraying the efficient low-residue insecticide on the trapping plants. The combined prevention and control method has the characteristics of low cost, environment friendliness, high efficiency, no pesticide residue and the like, and has a wide application prospect.
Description
Technical Field
The invention belongs to the technical field of agricultural and forestry insect biology, and particularly relates to a method for preventing and controlling thrips pests by utilizing combination of trapping plants, pesticides and repellents.
Background
Thrips is a collective name of Thysanoptera (Thysanoptera) insects, and is also a worldwide pest which seriously damages various crops such as fruits, vegetables, flowers and the like. The insects are tiny and are hidden in flowers and tender leaves. At present, more than 7700 thrips are recorded worldwide, more than 570 kinds of thrips are recorded in China, more than 600 plants can be damaged, and various important agricultural economic crops are seriously damaged.
At present, chemical prevention and control methods are still mainly adopted for preventing and controlling thrips at home and abroad. Because the insects are hidden in flowers, the chemical is difficult to control, so that the ideal control effect is difficult to obtain by a single chemical control method. If thrips are driven out of the flower, the chemical agent is assisted for control, the control effect can be improved, and the insect resistance to the chemical agent can be controlled. Meanwhile, thrips pests are various in variety, and at present, a single component with high effect of avoiding various thrips pests is difficult to find, so that a composite repellent is needed.
Disclosure of Invention
The invention aims to provide a method for preventing and controlling thrips pests by utilizing the combination of trapping plants, pesticides and repellents, which has the characteristics of low cost, environmental protection, high efficiency, no pesticide residue and the like; the used trapping plants are ornamental plants, and have high safety performance, so that the trapping plants have wide application prospect.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a method for preventing and controlling thrips pests by combining trapping plant, insecticide and repellent includes such steps as suspending the repellent above the crop to be protected by 5-10-cm a distance from top of the crop, planting a row of plants able to trap thrips on both sides of the crop to be protected, repelling thrips to the trapping plant, spraying insecticide to the trapping plant, and killing the trapped thrips thoroughly by the toxicity of insecticide.
Further, the repellent is prepared by taking liquid paraffin as a solvent, respectively preparing single component solutions of 100 mug/ml of beta-ionone, cis-4-heptenol, alpha-terpinene, 4-isopropyl toluene and dibutyl hydroxy toluene, and then mixing the solutions according to the same mass.
Further, the repellent is specifically dropwise added into a cylinder container without a cover, wherein the diameter of the cylinder container is 3cm, the height of the cylinder container is 5cm, and the depth of the cylinder container is 2cm, and then the cylinder container is hung.
Further, small holes with the diameter of 0.3cm are formed in two symmetrical sides of the upper part of the cylinder container and are used for hanging and fixing through ropes.
Further, the cylindrical container is made of composite rubber, and has a good slow release effect.
Further, the trapping plant is vervain or pseudo-weeping forsythia with better trapping effect on thrips pests.
Further, the pesticide is cyantraniliprole or emamectin benzoate with high efficiency and low toxicity.
The prevention and control method can be used for preventing and controlling thrips pests such as frankliniella occidentalis, yellow thrips, frankliniella occidentalis, palmi thrips, thrips macrocarpium and the like.
The invention has the remarkable advantages that:
(1) The plant source substance used as the repellent has various types, complex structure, wide action target sites and strong effect complementation; the used trapping plants have stronger trapping effect on thrips pests.
(2) The method has the characteristics of low cost, environmental friendliness, safety, no pollution and the like, and the repellent components are all plant source natural compounds, so that insect resistance is not easy to cause; the prevention and control process has the advantages of repelling, trapping and poisoning, and definite function, and can thoroughly kill thrips and prevent the thrips from escaping again to be harmful.
Detailed Description
In order to make the contents of the present invention more easily understood, the technical scheme of the present invention will be further described with reference to the specific embodiments, but the present invention is not limited thereto.
Example 1
1. Preparation method of thrips repellent
1.1 Preparation of a Single component solution
Preparation of beta-ionone solution: preparing 10 mu g/mu l of beta-ionone standard solution by taking liquid paraffin as a solvent, then adding 10 mu l into 990 mu l of liquid paraffin, and uniformly mixing to obtain 100 mu g/ml of beta-ionone solution;
preparation of cis-4-heptenol solution: preparing 10 mu g/mu l of cis-4-heptenol standard solution by taking liquid paraffin as a solvent, then adding 10 mu l into 990 mu l of liquid paraffin, and uniformly mixing to obtain 100 mu g/ml of cis-4-heptenol solution;
preparation of a-terpinene solution: preparing 10 mu g/mu l of alpha-terpinene standard solution by taking liquid paraffin as a solvent, then adding 10 mu l into 990 mu l of liquid paraffin, and uniformly mixing to obtain 100 mu g/ml of alpha-terpinene solution;
preparation of 4-isopropyl toluene solution: preparing 10 mu g/mu l of 4-isopropyl toluene standard solution by taking liquid paraffin as a solvent, then adding 10 mu l into 990 mu l of liquid paraffin, and uniformly mixing to obtain 100 mu g/ml of 4-isopropyl toluene solution;
preparation of a dibutyl hydroxy toluene solution: preparing 10 mu g/mu l of dibutyl hydroxy toluene standard solution by taking liquid paraffin as a solvent, then adding 10 mu l into 990 mu l of liquid paraffin, and uniformly mixing to obtain 100 mu g/ml dibutyl hydroxy toluene solution;
1.2 Preparation of thrips repellent
Mixing 100 mug/ml of beta-ionone solution, cis-4-heptenol solution, alpha-terpinene solution, 4-isopropyl toluene solution and dibutyl hydroxyl toluene solution obtained in the step (1) according to the same mass to obtain the repellent;
1.3 Preparation of thrips repellent formula 1
Mixing 100 mug/ml of beta-ionone solution, cis-4-heptenol solution, alpha-terpinene solution, 4-isopropyl toluene solution and dibutyl hydroxyl toluene solution obtained in the step (1) according to a mass ratio of 1:3:2:1:2 to obtain a repellent formula 1;
1.4 Preparation of thrips repellent formula 2
Mixing the beta-ionone solution, the cis-4-heptenol solution, the alpha-terpinene solution, the 4-isopropyl toluene solution and the dibutyl hydroxyl toluene solution which are obtained in the step (1) according to the mass ratio of 1:2:3:2:1 to obtain a repellent formula 2;
2. thrips response to the antenna potential of the components and the repellent of the invention, formulation 1 and formulation 2
And uniformly dripping 20 mu l of beta-ionone solution, cis-4-heptenol solution, alpha-terpinene solution, 4-isopropyl toluene solution and dibutyl hydroxyl toluene solution on V-shaped filter strips with the length of 5cm and the width of 0.5cm respectively, placing the V-shaped filter strips into a Pasteur dropper, connecting the tail end of the dropper with a stimulation airflow control device, and enabling the mouth of the air supply pipe to be vertical to the antenna longitudinally and be 1cm away from the antenna. The continuous gas flow rate was adjusted to 124ml/min, the stimulation gas flow rate was adjusted to 20ml/min, and the stimulation time was 0.2s each time. The interval between the two stimulations is 40s to ensure complete restoration of the feeler-sense organ function. Each sample solution was stimulated 3 times in parallel on one antenna to average, and at least 5 antennas were tested in duplicate. Liquid paraffin is used as a control, and for each sample to be tested, the liquid paraffin, the sample to be tested and the liquid paraffin are stimulated in sequence, and the average value is obtained by the two controls. The results of the antenna potential reaction are shown in Table 1.
EAG relative reaction value = EAG relative reaction value of the sample to be measured-average EAG relative reaction value of liquid paraffin.
TABLE 1 response of thrips to antenna potential of single component, inventive repellent, formulation 1, formulation 2
As shown in Table 1, the yellow chest thrips has stronger antennal potential response (the highest relative response value is alpha-terpinene-88.13 mu V, and the lowest relative response value is beta-ionone-66.64 mu V) to all 5 single components; the relative value of the response of the antenna potential of the thrips flavus to the repellent reaches-227.45 mu V at most, which is obviously higher than the EAG response value of formula 1 (-156.13 mu V) and formula 2 (-159.16 mu V). The thrips palmi has stronger antennal potential reaction (the highest relative reaction value is alpha-terpinene-83.33 mu V and the lowest relative reaction value is beta-ionone-61.84 mu V) on all 5 single components; the relative value of the response of the antenna potential of the thrips flavus to the repellent reaches-218.85 mu V at most, which is obviously higher than the EAG response value of formula 1 (-152.73 mu V) and formula 2 (-153.96 mu V). The above shows that the formulation of the repellent has stronger electrophysiological reaction.
Example 2
The directional behavioral response of frankliniella occidentalis or thrips flavus to the compound is measured by a Y-type olfactometer (the Y-type olfactometer is obtained by improving the application of an authorized patent number ZL201020239767.7 according to the basic principle of the olfactometer). The length of the main arm of the Y-shaped tube is 20cm, the lengths of the two arms are 10cm respectively, and the included angle of the two arms is 90 ℃. The ends of the two arm tubes are sequentially connected with a distilled water bottle and an active carbon bottle by a silica gel tube, and finally the active carbon bottle is opened in the atmosphere, and the insect release bottle is connected with an air extractor by gauze. The filter strips were cut into 5cm ×1cm, folded into a "V" shape, and placed in a trap inlet glass tube.
The Y-shaped olfactometer is inclined by 30 degrees, and the temperature is controlled at 25+/-2 ℃. 100 mu L of the repellent and the repellent formula 1 and the repellent formula 2 of the invention are respectively taken, evenly dripped on a filter paper strip and put into a taste source bottle on one side, and the filter paper with 100 mu L of liquid paraffin dripped on the other side is taken as a reference, the flow rate of the air suction airflow is 1L/min, and the airflows respectively entering two pipes are filtered by active carbon and humidified by distilled water.
3 day old frankliniella occidentalis or yellow chest thrips adults are taken to be placed in insect release bottles, 30 frankliniella occidentalis and yellow chest thrips are measured separately each time, the measurement is repeated 3 times, absolute ethyl alcohol is dipped in cotton balls after each measurement to wipe the inner wall and the outer wall of a Y-shaped tube and are dried, and then two arms of the Y-shaped tube are exchanged with a taste source bottle and a comparison to eliminate the possible influence of geometric errors of the two arms on the action of the thrips. Putting the test insects into an insect release bottle of a Y-shaped pipe to start the test, enabling each test insect to climb over 1/2 of a selection arm and stay for more than 10 s, namely, calculating to make a selection, considering thrips staying on a main arm of the Y-shaped pipe as non-reaction, counting experimental results within 30 minutes, and calculating the evasion rate according to the following formula.
The rate of repellency/% = (number of insects in control tube-number of insects in treatment tube)/total number of insects x 100.
The result shows that the repellent of the invention has the repellent rate of 76.67%, 50.00% and 53.33% on the thrips flavus in formula 1 and formula 2, and the repellent amount of the repellent of the invention is 70%, 45.56% and 50% on the thrips frankliniella occidentalis respectively, which shows that the repellent effect of the invention is obviously higher than that of formula 1 and formula 2.
From the results, the repellent has remarkable repellent effect on thrips. The indoor behavior selection test on other thrips is similar in repellent effect.
Example 3
Specific test method referring to example 2, the upper plant of the trapping plant verbena was placed in a Y-shaped olfactometer flavor source bottle and the picked host plant cucumber leaf was used as a reference. The result shows that the trapping amount of the trapping plant verbena to frankliniella occidentalis is 22.33, and the cucumber leaves are only 4, so that the trapping plant defined by the invention has a good trapping effect on frankliniella occidentalis.
Example 4
The test site selects Fujian Minhou garden greenhouse, and the crops are strawberries. Blue armyworm plates are adopted as trapping devices, the repellent and the repellent formula 1 and formula 2 are respectively placed in composite rubber bodies with the diameter of 3cm, the height of 5cm and the depth of 2cm, are hung above strawberries by 10cm, the span is 6-8 m, 3 rows of the strawberries are hung on each shed, the row spacing is 1.5 m, the color plate spacing is 3 m, one row of vervain is planted on each side of the strawberries as trapping plants, methylaminoavermectin benzoate is adopted as an insecticide, and the experiments 3d and 14d are respectively sprayed on the trapping plants once. Experiments were performed in 3 replicates, with random arrangements between different treatments. Before the test, the cardinality of the thrips flavus in the strawberry garden is respectively investigated, 3 points are selected in each cell for 3d, 7d and 14d, the number of the thrips flavus is respectively investigated, and finally the result is counted. And taking another garden greenhouse as a blank control.
Calculating the rate of reduction of insect population, correcting the control effect and testing the difference significance according to the number of live thrips before and after the control of the treatment area and the control area by adopting the Duncan's new compound range ' DMRT ' method.
The result shows that the prevention effect of the repellent on the yellow chest thrips in 3d, 7d and 14d is 70.81%, 92.10% and 94.82% respectively by using the induced plants and suspending the repellent, which shows that the thrips amount is rapidly reduced, the repellent has good prevention effect, and the total death of the thrips can be caused by spraying emamectin benzoate on the induced plants. The number of thrips in the blank strawberry garden is 91.5, 92.5, 88 and 81.75 in sequence along with the time, and the blank strawberry garden is basically stable.
The foregoing description is only of the preferred embodiments of the invention, and all changes and modifications that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (6)
1. A method for preventing and controlling thrips pests by utilizing the combination of trapping plants, pesticides and repellents is characterized by comprising the following steps: suspending the repellent above the protected crop at a distance of 5-10 cm from the top of the protected crop, planting a row of plants capable of trapping thrips on each side of the protected crop, and then spraying insecticide on the trapping plants so as to thoroughly kill the trapping thrips;
the repellent is prepared by taking liquid paraffin as a solvent, respectively preparing single component solutions of 100 mug/ml of beta-ionone, cis-4-heptenol, alpha-terpinene, 4-isopropyl toluene and dibutyl hydroxy toluene, and then mixing the solutions according to the same mass.
2. The method for controlling thrips pests by using the combination of trapping plants, pesticides and repellents according to claim 1, which is characterized in that: the repellent is specifically characterized in that the repellent is firstly dripped into a cylinder container without a cover, the diameter of which is 3cm, the height of which is 5cm and the depth of which is 2cm, and then the cylinder container is hung.
3. The method for controlling thrips pests by using the combination of trapping plants, pesticides and repellents according to claim 2, which is characterized in that: and small holes with the diameter of 0.3cm are formed in two symmetrical sides of the upper part of the cylinder container and are used for hanging and fixing through ropes.
4. The method for controlling thrips pests by using the combination of trapping plants, pesticides and repellents according to claim 2, which is characterized in that: the cylindrical container is made of composite rubber.
5. The method for controlling thrips pests by using the combination of trapping plants, pesticides and repellents according to claim 1, which is characterized in that: the trapping plant is herba Verbenae or fructus forsythiae.
6. The method for controlling thrips pests by using the combination of trapping plants, pesticides and repellents according to claim 1, which is characterized in that: the pesticide is cyantraniliprole or emamectin benzoate.
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