CN110313476B - Method for preventing and controlling adult willow Blueleaf beetles and repellent used by method - Google Patents
Method for preventing and controlling adult willow Blueleaf beetles and repellent used by method Download PDFInfo
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- CN110313476B CN110313476B CN201910609068.2A CN201910609068A CN110313476B CN 110313476 B CN110313476 B CN 110313476B CN 201910609068 A CN201910609068 A CN 201910609068A CN 110313476 B CN110313476 B CN 110313476B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- C07C29/76—Separation; Purification; Use of additives, e.g. for stabilisation by physical treatment
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Abstract
The invention discloses a method for preventing and controlling adult willow Blueleaf beetles and a repellent used by the method, and belongs to the technical field of insect repellent. The method for preventing and controlling adult willow blue leaf beetles is to apply trans-3-hexenol or a solution of trans-3-hexenol to willows. The invention adopts the solid-phase micro-extraction head to extract trans-3-hexenol from the leaves of the willow, the preparation has long release period and simple preparation, and has obvious effect of avoiding the adult willow leaf beetle. The trans-3-hexenol as an effective component exists in partial plants in the nature, is harmless to human and livestock, is environment-friendly and has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of insect repellent agents, and particularly relates to a method for controlling adult willow Blueleaf beetles and a repellent agent used by the method.
Background
Willow is the most widely used tree species in urban and rural areas and landscaping in China, and willow herb (platicotre versicolor) is a leaf-eating pest which has the widest harm range and the most serious harm degree to willow species. The willow herb belongs to the genus Chrysanthemum of the family Lysimalidae (Chrysomelidae) of the order Coleoptera (Coleoptera), eats the leaves and tender tips of willow, most willow species are harmed by the Salix herb, and the Salix herb comprises Salix longituba, Salix integra, Salix babylonica and the like. In southern provinces in China, the insect pest occurs for 8-9 generations every year, and can cause seedling death when the damage degree is serious, thereby having great damage to the seedling cultivation and production of willows.
At present, the control method of the willow blue leaf beetle mainly depends on an insecticide and artificial killing, and comprises methods of tree crown spraying, trunk ring resistance, soil turning in winter, shaking and killing and the like. However, the commonly used broad-spectrum insecticide has high cost and low pertinence, even causes the decrease of the natural enemies of the willow blue leaf beetle to generate negative effects, and is easy to cause the drug resistance of insects after long-term use. Salix plants are widely used in landscaping, the pest mainly occurs in parks, scenic spots and other places, and the use of broad-spectrum pesticides can damage the environment and pose a threat to human health. Meanwhile, the artificial trapping and killing efficiency is low, and the artificial trapping and killing method is difficult to apply to actual production practice. Therefore, the development of specific pesticides with strong pertinence and environmental friendliness is a problem to be solved urgently in production. Numerous studies have shown that: the natural attractant or repellent of the pests is utilized to prevent and treat the pests, so that the prevention and treatment efficiency is high, and the environment is protected. Therefore, the search of the information compound of the willow blue leaf beetle is the key for developing specific pesticides.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the defects in the prior art, the invention aims to provide the method for preventing and controlling the adult willow hornet, which has the advantages of environmental protection, no pollution and no harm to trees. The invention also aims to provide the repellent for preventing and controlling the adult willow Blueleaf beetles, which has the characteristics of strong pertinence and environmental friendliness.
The technical scheme is as follows: in order to solve the problems, the technical scheme adopted by the invention is as follows:
a method for preventing and treating adult Salix integra Blanco comprises applying trans-3-hexenol or trans-3-hexenol solution to Salix integra.
In the method for controlling adult willow Blueleaf beetles, the trans-3-hexenol solution is a paraffin oil solution of trans-3-hexenol, and the concentration is 0.001-1 mol/L.
The method for controlling adult willow Blueleaf beetles is characterized in that the concentration of the trans-3-hexenol alcohol solution is 0.1 mol/L.
An insect repellent for preventing and treating adult blue leaf beetle of Salix purpurea is trans-3-hexenol or trans-3-hexenol solution.
The repellent agent for preventing and treating adult willow Blueleaf beetles is characterized in that the trans-3-hexenol solution is paraffin oil solution of trans-3-hexenol, and the concentration of the paraffin oil solution is 0.001-1 mol/L.
The concentration of the anti-3-hexenol solution is 0.1 mol/L.
The repellent for preventing and treating the willow blue leaf beetle adults is obtained by extracting trans-3-hexenol from leaves of salix triphylla.
The preparation method of trans-3-hexenol comprises picking fresh leaves of Trifolium Pratentis, placing in an extraction flask, and sealing the top of the extraction flask with a preservative film; adsorbing with solid phase microextraction head, and soaking in 40-50 deg.C constant temperature water bath for 30-40 min; the solid phase micro-extraction head is treated for 3-5min at the temperature of 200-250 ℃ for desorption treatment to obtain trans-3-hexenol.
According to the preparation method of the trans-3-hexenol, the solid-phase micro-extraction head is a 65-micron Polydimethylsilane (PDMS)/Divinylbenzene (DVB) manual solid-phase micro-extraction head.
Has the advantages that: compared with the prior art, the invention has the advantages that:
(1) the inventive adult diaphorina latifolia repellent has long release period, and the service life of the trap as a field insect trap can reach 2-4 weeks. The composition is simple to prepare, has obvious avoidance effect on the adult willow blue leaf beetles, is a natural component in plants, is safe to people and livestock, and has no pollution to the environment.
(2) The invention provides important technical support for artificial control of the adult willow blue leaf beetles, has wide application prospect and good practical application value, and can generate better economic benefit and social benefit.
Drawings
FIG. 1 is a GC-MS analysis spectrum of a willow leaf extract, wherein FIG. a is a chart of the detection result of a dustpan willow leaf extract, and FIG. b is a chart of the detection result of a thaumax trilobata leaf extract;
FIG. 2 is a graph showing the evasion effect of paraffin oil solution of trans-3-hexenol on adult Salicornia caerulea without feeding leaves;
FIG. 3 is a graph showing the evasion effect of paraffin oil solution of trans-3-hexenol on adult Salicornia caerulea under the condition of adding feeding leaves.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
GC-MS test: a Trace ISQ-LT gas chromatograph-mass spectrometer is adopted, the GC analysis condition is a DB-5MS (30m multiplied by 0.25mm multiplied by 0.25 mu m) elastic quartz capillary column, the carrier gas is high-purity helium, the flow rate is 1mL/min, and split-flow sample injection is not carried out. Sample inlet temperature 250 ℃, column temperature program: the initial temperature was 40 deg.C, held for 2min, ramped up to 250 deg.C at 6 deg.C/min, held for 5 min. MS analysis conditions: EI ion source, electron energy is 70eV, ion source temperature is 250 ℃, and scanning range is 45-450U.
Example 1
Picking fresh leaves of the willow with the three pistil and the willow with the dustpan 5g respectively, and placing the leaves in 50mL extraction bottles respectively for extraction. The specific operation process is as follows: the top of the bottle is sealed by a preservative film, a 65-micron PDMS/DVB manual solid-phase micro-extraction head (Supelco) is used for adsorption, and the extraction bottle is placed in a constant-temperature water bath at 40 ℃ for 40 min. And (3) treating the solid phase micro-extraction head at 250 ℃ for 3min for desorption treatment, and respectively carrying out GC-MS analysis on the obtained substances. The results of the analysis are shown in FIG. 1. As can be seen from FIG. 1, the retention time of the differential substance was 10.17 min. By comparing the compound database, the compound represented by this peak is trans-3-hexenol.
Example 2
Under the condition of not adding feeding leaves, the adult willow blue leaf beetle selectively measures the repellent and a control:
the concentration of the paraffin oil solution of the repellent, trans-3-hexenol, used included 0.001, 0.01, 0.1 and 1 mol/L.
The preparation method of the solution comprises the following steps: firstly, preparing a paraffin oil solution of trans-3-hexenol with 1 mol/L: 5mL of trans-3-hexenol was added to an empty 50mL centrifuge tube, the volume was adjusted to 50mL, and the mixture was shaken and mixed. Then, 1mL, 0.1mL and 0.01mL of the prepared 1mol/L paraffin oil solution of trans-3-hexenol are respectively taken out by a pipette gun and added into the air centrifuge tube, and the volume is determined to be 10mL, thus preparing the paraffin oil solutions of the trans-3-hexenol of 0.1, 0.01 and 0.001 mol/L.
The determination method comprises the following steps:
1. the long arm of the Y-shaped olfactometer is 10cm long, the two short arms are 6cm long, the included angle between the two arms is 75 degrees, the diameter is 1cm, and the end point lines are arranged at the positions 5cm away from the two short arms. The flow rate was controlled at 0.8L/min. An artificially cultivated population of willow blue beetles that has emerged for more than 4 days to reach sexual maturity is used for this test.
2. Adding 10mL of repellent into one flavor source bottle, using 10mL of paraffin oil as a reference, connecting a beetle from a nozzle of a long arm each time, observing for 5min to see whether the beetle can reach a finish line on a short arm, and recording a selection result if the beetle can reach the finish line on the short arm; if not, it is considered as unselected.
3. 100 adult worms are used in each group of test, and each 10 beetles is processed, the Y-shaped tube is replaced, and the replaced Y-shaped tube is firstly cleaned by absolute ethyl alcohol and then is placed into an oven for drying after being washed by distilled water. So as to eliminate the influence of the environment and the pheromone of the willow blue leaf beetle on the experimental result.
4. The whole measurement is carried out in a dark room with a unique light source to ensure uniform illumination when selecting the two arms.
The results are shown in FIG. 2, with the horizontal axis showing the number of selections (heads) of each individual source of the adult Salicornia caerulea, the number of heads of each adult test is 100, the number of heads listed in each test in the figure is subtracted, and the remaining individuals are the number of heads of insects for which no selection was made in each test. As can be seen from FIG. 2, the paraffin oil solutions of 0.001, 0.01, 0.1 and 1mol/L trans-3-hexanol all can repel the adult Bluebeetle, wherein the paraffin oil solutions of 0.1mol/L and 1mol/L trans-3-hexenol have better repelling effect, but the repelling effect is not obviously different. Therefore, in practice, a solution with a lower concentration is selected. The results show that: paraffin oil solutions of trans-3-hexenol at 0.1mol/L and 1mol/L both showed significant repellent effects on adult Salicornia bigelovii.
Example 3
Selectivity determination of adult Salicarpa caerulea on repellent and control under the condition of adding feeding leaf
Willow leaves which are favored by the willow herb are added into the repellent and the control component respectively in the comparative example 1, and the selectivity measurement is carried out.
Concentrations of the repellent used included: 0.001, 0.01, 0.1 and 1mol/L of trans-3-hexenol in paraffin oil.
The preparation method of the solution comprises the following steps: firstly, preparing a paraffin oil solution of trans-3-hexenol with 1 mol/L: 5mL of trans-3-hexenol was added to an empty 50mL centrifuge tube, the volume was adjusted to 50mL, and the mixture was shaken and mixed. Then, 1mL, 0.1mL and 0.01mL of the prepared 1mol/L paraffin oil solution of trans-3-hexenol are respectively taken out by a pipette gun and added into the empty centrifuge tube, and the volume is determined to be 10mL, thus preparing the paraffin oil solutions of the trans-3-hexenol of 0.1mol/L, 0.01mol/L and 0.001 mol/L.
The determination method comprises the following steps:
1. placing wet filter paper in two uncovered culture dishes, covering a layer of gauze, and respectively placing dustpan willow leaves with similar growth states on the gauze.
2. A paraffin oil solution of trans-3-hexenol at the above concentration was added to the petri dish-wetted filter paper at one end and care was taken not to drop onto the leaves. Then 10 head willow blue leaf beetle adults are put in the culture dish, the selection time is 15min, the head number of the beetles in 2 culture dishes is recorded, and the avoidance effect of the trans-3-hexenol is tested. After the test is finished, 10 new adult willow blue leaf beetles are replaced, 100 adult willow blue leaf beetles are made in each group of test, the specific result is shown in figure 3, the number of heads of worms listed in each test in figure 3 is subtracted, and the remaining individuals are the number of heads of worms which are not selected in each test.
3. Paraffin oil solutions of trans-3-hexenol at 1, 0.1, 0.01 and 0.001mol/L were prepared and assayed for adult Salicornia caerulea in sequence as described above.
4. The above experiment was performed by spreading paraffin oil on filter paper as a control, and the other treatment methods were the same.
As can be seen from fig. 3: paraffin oil solutions of trans-3-hexenol of 0.01, 0.1 and 1mol/L have obvious avoidance effect on the adult hyalospora caerulea, and the avoidance effect is 66 heads (paraffin oil + salifolium dustpan leaf control group): 34 heads (paraffin oil solution of trans-3-hexenol of 0.01mol/L + salix dustpan leaves treatment group), 77 heads (paraffin oil + salix dustpan leaves control group): 23 (paraffin oil solution of trans-3-hexenol of 0.1mol/L + salix dustpan leaves treatment group) and 81 (paraffin oil + salix dustpan leaves control group): 19 heads (1mol/L paraffin oil solution of trans-3-hexenol + salix dustpan leaf treatment group). Considering the effect and the cost comprehensively, the trans-3-hexenol paraffin oil solution of 0.1mol/L is preferably selected as the applicable concentration of the adult pest repellent of the willow blue leaf beetle.
Claims (4)
1. A method for preventing and treating adult willow Blueleaf beetles is characterized in that trans-3-hexenol or trans-3-hexenol solution is applied to willows; the preparation method of the trans-3-hexenol comprises the following steps: picking fresh leaves of the willow, placing the leaves in an extraction flask, and sealing the top of the extraction flask by using a preservative film; adsorbing with solid phase microextraction head, and soaking in 40-50 deg.C constant temperature water bath for 30-40 min; the solid phase micro-extraction head is treated for 3-5min at the temperature of 200-250 ℃ for desorption treatment to obtain trans-3-hexenol.
2. The method for controlling adult willow hornleaf beetles according to claim 1, wherein the trans-3-hexenol solution is a paraffin oil solution of trans-3-hexenol, and the concentration is 0.001-1 mol/L.
3. The method for controlling adult willow hornet according to claim 1 or 2, characterized in that the concentration of the trans-3-hexenol solution is 0.1 mol/L.
4. The method of claim 1, wherein the solid phase microextraction head is a 65 μm PDMS/DVB manual solid phase microextraction head.
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| EP1708570B1 (en) * | 2004-01-30 | 2012-08-08 | Freie Universität Berlin | Composition and use thereof as attractant for the garden chafer (Phyllopertha horticola) |
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