CN111202057A

CN111202057A - Insecticidal pesticide composition containing diatomite with special structure and application thereof

Info

Publication number: CN111202057A
Application number: CN202010104248.8A
Authority: CN
Inventors: 陈瑞明
Original assignee: Benson Huizhou Biotechnology Co Ltd
Current assignee: Benson Huizhou Biotechnology Co Ltd
Priority date: 2020-02-20
Filing date: 2020-02-20
Publication date: 2020-05-29

Abstract

The invention relates to the technical field of pesticides, and particularly relates to an insecticidal pesticide composition containing diatomite with a special structure and application thereof. An insecticidal pesticide composition containing diatomite with a special structure comprises the diatomite with the special structure and an insecticide, wherein the weight ratio of 1: (0.0005-50); the diatomite with the specific structure has a sawtooth-shaped honeycomb structure. The invention also provides an insecticidal pesticide water dispersible granule containing the diatomite with the special structure, which comprises 70-90% of insecticidal pesticide composition, 5-25% of auxiliary agent and 100% of filler in percentage by weight. When the insecticidal pesticide water dispersible granule provided by the invention is applied to the surface of crops, the insecticidal pesticide water dispersible granule has higher spreadability and adhesiveness, the wetting, spreading, dispersing, detenting and penetrating properties of a liquid medicine are obviously improved, and the loss caused by the drift of the liquid medicine along with wind (airflow) is reduced; meanwhile, the dosage of the effective components of the chemical pesticide is greatly reduced, the compatibility of the chemical pesticide with the environment is improved, and the ecological environment is protected.

Description

Insecticidal pesticide composition containing diatomite with special structure and application thereof

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to an insecticidal pesticide composition containing diatomite with a special structure and application thereof.

Background

Because agricultural pests can cause great loss to crops, the control of the pests is an important means for improving the quality and the yield of the crops, and the pests damage the growth of the growing crops, so that the yield is obviously reduced, the cost is wasted, and the method is extremely unfavorable for agricultural production. The current pesticide is used in a large amount, has serious damage to crops, hastens the resistance to be increased rapidly, greatly reduces the control effect on pests and causes serious pollution to the environment; the method aims to improve the control effect of pests, cannot increase the usage amount and the drug resistance of the pesticide, and provides a new challenge for the pesticide.

Because people use single-dosage chemical insecticide for a long time, pests are easy to generate drug resistance, in order to achieve the pest control effect, higher-toxicity pesticide has to be used or the using amount of the pesticide is increased, so that the economic burden of farmers is increased, the ecological environment is polluted, the ecological balance is destroyed, the safety of agricultural products is affected, and a large number of natural enemies of the pests are killed, so that the pests are rampant. At present, a large amount of mixed insecticides are available in the market, most of the mixed insecticides are chemical pesticides, the toxicity to people and livestock is high, and pests can generate drug resistance after long-time use. A large number of field experiments prove that the combination of two medicines with different action mechanisms can not only delay the generation of resistance, but also improve the pesticide effect and the residual time, reduce the use amount, reduce the pesticide application cost of farmers, improve the pesticide effect and the duration while reducing the environmental pollution, reduce the labor cost of the farmers and obtain good economic and social benefits. Different components are compounded, and whether a certain compound has synergism, addition or antagonism is judged according to the actual application effect. In most cases, the compounding effect of the pesticide is additive effect, the compounding with real synergistic effect is less, and especially the compounding with very obvious synergistic effect and very high co-toxicity coefficient is less.

The physical pesticide has completely different mechanism from the existing chemical pesticide, has extremely strong novelty and uniqueness, is suitable for the great trend of 'reduction and synergism' of chemical pesticide in China, and has better research, development and application prospects. In the invention, the diatomite with a special structure and the chemical insecticide are compounded for use by the inventor, so that the effect is improved, and the product has wide application prospect and considerable economic benefit.

Drawings

Fig. 1 is an optical microscope photograph of a specific-structure diatomaceous earth having a saw-toothed honeycomb structure in example 1 of the present invention.

FIG. 2 is an electron microscope image of diatomaceous earth having a specific structure of a saw-toothed honeycomb structure in example 1 of the present invention.

Disclosure of Invention

In order to solve the technical problems, the first aspect of the invention provides an insecticidal pesticide composition containing diatomite with a special structure, which comprises the diatomite with the special structure and an insecticide in a weight ratio of 1: (0.0005-50); the diatomite with the specific structure has a sawtooth-shaped honeycomb structure.

As a preferable technical scheme, the pesticide is any one of thiamethoxam, clofentezine and permethrin.

As a preferable technical scheme, the weight ratio of the diatomite with the specific structure to the thiamethoxam is 1: 40-100: 1.

as a preferable technical scheme, the weight ratio of the diatomite with the specific structure to the clofentezine is 1: 40-100: 1.

as a preferable technical scheme of the invention, the weight ratio of the diatomite with the specific structure to the permethrin is 1: 40-600: 1.

as a preferable technical scheme, the formulation of the insecticidal pesticide composition is selected from any one of wettable powder, water dispersible granules, suspending agents, missible oil, emulsion in water, powder, granules, effervescent tablets and dispersible tablets.

The invention provides a water dispersible granule of an insecticidal pesticide containing diatomite with a special structure, which is prepared from the raw materials of 1-5 of the insecticidal pesticide composition; the pesticide composition comprises 70-90% of pesticide composition, 5-25% of auxiliary agent and 100% of filler in percentage by weight; the auxiliary agent comprises at least one of a dispersing agent, a wetting agent, a stabilizing agent, a disintegrating agent, a separating agent, a stabilizing agent and a binder.

As a preferred technical solution of the present invention, the binder comprises a combination of phenolic resin, polyethylene glycol and citric acid, and the weight ratio is 1: (0.3-1): (0.8-1.5).

The third aspect of the present invention provides a preparation method of the pesticidal pesticide composition, comprising the steps of:

(1) mixing the auxiliary agent and the filler, then adding the insecticide, uniformly mixing and crushing to obtain a mixed material;

(2) mixing the mixture with diatomaceous earth with specific structure, shaking for 5-20min, and stirring for 20-40min to mix well to obtain mother powder;

(3) kneading the mother powder, granulating, drying and screening to obtain the product.

The fourth aspect of the invention provides application of the insecticidal pesticide water dispersible granule, which can be applied to control of red spiders, scale insects, frankliniella occidentalis, trialeurodes vaporariorum, liriomyza, prodenia litura, leafhoppers, tea yellow thrips, tea geometrid, lygus lucorum and grape aphids.

Has the advantages that: the pesticide composition provided by the invention is applied to the surfaces of crops, has higher spreadability and adhesiveness, obviously improves the wetting, spreading, dispersing, detention and permeability of the pesticide liquid, reduces the loss of the pesticide liquid caused by drift along with wind (airflow), and further achieves the purpose of improving the biological activity of the pesticide liquid; and secondly, the dosage of the effective components of the chemical pesticide is greatly reduced, the compatibility of the chemical pesticide with the environment is improved, the cost is reduced, and the pesticide has an important effect on improving the quality of a pesticide preparation and has the aim of protecting the ecological environment.

Detailed Description

The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

In the present invention, the term "composition" means that the components of the composition are packaged in combination or separately and used together when used. That is, in the present invention, the "diatomaceous earth and pesticide of specific structure" means that diatomaceous earth and pesticide of specific structure can be mixed and packaged together, and used together when in use; also comprises specific structure diatomite and pesticide which are separately packaged and mixed together according to proportion when in use.

Diatomite with specific structure

In the present invention, the diatomaceous earth having a specific structure has a zigzag honeycomb structure.

The diatomite with the specific structure is provided withDiatomaceous earth of a specific crystal structure. General diatomaceous earth contains a small amount of Al in addition to silica as a main component₂O₃、Fe₂O₃CaO, MgO, etc., and organic matter. The diatomite with the specific structure is diatomite with specific saw-tooth honeycomb structure silicon dioxide in main content after purification treatment.

Preferably, the diatomaceous earth of a particular structure is purchased from Yirui Stone, France, under the designation Celite 610.

In the present invention, the diatomite with the specific structure, as shown in fig. 1 and 2, can be seen that the Celite610 has a clear saw-tooth honeycomb structure under an optical microscope and an electron microscope.

The inventor believes that the diatomite with the specific structure has a larger specific surface area due to the saw-toothed honeycomb structure, is beneficial to better adsorbing a waxy layer on the surface of an insect body and causes the insect body to be quickly dehydrated; on the other hand, due to the zigzag honeycomb structure, the composition is beneficial to causing damage to a wax layer on the surface of the insect body, tearing the surface of the insect body, rapidly permeating into the insect body and causing fatal damage to the insect, thereby improving the lethality of the composition.

Insecticide

In the invention, the chemical insecticide is any one of thiamethoxam, clofentezine and permethrin.

Preferably, the weight ratio of the diatomite and the thiamethoxam with the specific structures is 1: 40-100: 1.

more preferably, the weight ratio of the physical insecticide to the thiamethoxam is 200: 1 or 400: 1.

preferably, the weight ratio of the diatomite and the clofentezine with the specific structure is 1: 40-100: 1.

more preferably, the weight ratio of the diatomite and the clofentezine with the specific structure is 50: 1.

preferably, the weight ratio of the diatomite with the specific structure to the permethrin is 1: 40-600: 1.

more preferably, the weight ratio of the diatomite with the specific structure to the permethrin is 300: 1 or 600: 1.

in the invention, after the insecticide and the diatomite with the sawtooth honeycomb structure are compounded and used, the co-toxicity coefficient of the composition to pests is obviously improved, and the quick action and the fatality rate are very high. The inventor thinks that the possible reason is that the physical damage such as adsorption dehydration and tearing of the silicon dioxide with the specific zigzag honeycomb unique structure to the insects can be utilized, and simultaneously, the penetration speed of the insecticide into the insects can be promoted, so that the insecticide can be quickly contacted with the insects and can penetrate into the insects, and the defect of slow effect caused by the characteristics such as no systemic absorption and contact killing of the insecticide is overcome. Under the synergistic effect of the pesticide and the diatomite with the specific structure, the control efficiency of the composition on pests is obviously improved, and the composition has high quick-acting property and lethality. The inventor finds that the pesticide composition has an obvious synergistic effect in a certain proportion range of the pesticide and the Celite610, the control effect of the composition is obviously improved compared with that of a single agent, the using dosage of chemical pesticides is reduced, the influence on the environment is reduced, the generation of drug resistance of diseases is delayed or overcome, and the comprehensive control of pests is facilitated.

The formulation of the diatomite-avermectin pesticide composition with a specific structure is not particularly limited in the invention, and various formulations known to those skilled in the art can be prepared and used by methods known to those skilled in the art.

Preferably, the dosage form of the insecticidal pesticide composition is any one selected from wettable powder, water dispersible granules, suspending agents, emulsifiable solutions, aqueous emulsions, powders, granules, effervescent tablets and dispersible tablets.

The invention provides a water dispersible granule of an insecticidal pesticide containing diatomite with a special structure, which is prepared from the following raw materials of the insecticidal pesticide composition; the pesticide composition comprises 70-90% of pesticide composition, 5-25% of auxiliary agent and 100% of filler in percentage by weight; the auxiliary agent comprises at least one of a dispersing agent, a wetting agent, a stabilizing agent, a disintegrating agent, a separating agent, a stabilizing agent and a binder.

Preferably, the pesticide water dispersible granule comprises 80% of pesticide composition, 15% of auxiliary agent and 100% of filler in percentage by weight;

auxiliary agent

In the invention, the auxiliary agent comprises at least one of a dispersing agent, a wetting agent, a stabilizing agent, a disintegrating agent, a separating agent, a stabilizing agent and a binder.

Preferably, the weight ratio of the dispersing agent, the wetting agent, the binder and the disintegrating agent is 1: (0.5-1.2): (0.8-1.5): (0.5-1).

More preferably, the weight ratio of the dispersing agent, the wetting agent, the binder and the disintegrating agent is 1: 1: 1.2: 0.8.

in the present invention, the dispersant is not particularly limited, and tea saponin, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, lignosulfonate, polycarboxylate, EO/PO block polyether, aralkyl phenol polyoxyethylene ether phosphate, alkyl naphthalene sulfonate, bis (alkyl) naphthalene sulfonate formaldehyde condensate, naphthalene sulfonic acid formaldehyde condensate, aryl phenol polyoxyethylene succinate sulfonate, octyl phenol polyoxyethylene ether sulfate, polycarboxylate, alkylphenol polyoxyethylene pyrimidine formaldehyde condensate sulfate, alkylbenzene sulfonic acid calcium salt, naphthalene sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene pyrimidine, fatty amine polyoxyethylene pyrimidine, fatty acid polyoxyethylene ester, ester polyoxyethylene pyrimidine and the like can be mentioned.

Preferably, the dispersant is sodium lignosulfonate.

In the present invention, the wetting agent is not particularly limited, and dodecyl sulfate, fatty alcohol polyoxyethylene ether sulfate, dibutyl naphthalene sulfonate, diisopropyl naphthalene sulfonate, alpha-alkenyl sulfonate, diisooctyl succinate sulfonate, dodecyl sulfonate, nonylphenol polyoxyethylene ether, alkylamido taurate, isooctyl alcohol polyoxyethylene ether, nekal, gleditsia sinensis lam powder, tea seed cake, silkworm excrement, soapberry powder, and the like can be mentioned.

Preferably, the wetting agent is sodium diisooctyl succinate sulfonate.

According to the invention, the sodium lignosulfonate and the sodium diisooctyl succinate sulfonate both have main chains with stronger hydrophobicity, and the hydrophobic part in the molecule is in a semi-surrounding shape, so that a hydrophobic cavity can be formed, the pesticide and the suspended particles of the diatomite with a specific structure can be effectively adsorbed and coated, and the hydrophobic cavity is provided with the same charges, so that the suspended particles can be more uniformly dispersed in water. Particularly, the diatomite with a specific structure selected in the invention has a saw-tooth honeycomb structure, the surface is extremely unsmooth, and the adjacent particles are easy to rub and agglomerate in the dispersing process to flocculate and precipitate, so that the suspension rate is reduced, and the effective utilization rate of the product is obviously reduced. The anionic dispersing agent and the wetting agent can avoid agglomeration and flocculation among particles, can fully wet target pests due to proper surface tension and contact angle, and improve the contact probability of effective components and targets, thereby obviously improving the pest control effect.

In the invention, the binder is at least one selected from gelatin, guar gum, Arabic gum, xanthan gum, sodium tripolyphosphate, phenolic resin, sodium alginate, sodium carboxymethylcellulose, white dextrin, methylcellulose, sodium acrylate, polyvinylpyrrolidone, cross-linked polyvinylpyrrolidone, polyethylene glycol, polyvinyl alcohol, anhydrous glucose, soluble starch, oxidized starch and citric acid.

Preferably, the binder is a combination of phenolic resin, polyethylene glycol, and citric acid.

Preferably, the weight ratio of the phenolic resin, the polyethylene glycol and the citric acid is 1: (0.3-1): (0.8-1.5).

More preferably, the weight ratio of the phenolic resin, the polyethylene glycol and the citric acid is 1: 0.8: 1.

preferably, the polyethylene glycol has a weight average molecular weight of greater than 2000.

More preferably, the polyethylene glycol has a weight average molecular weight of 4000.

The source of the polyethylene glycol is not particularly limited, and there may be mentioned, but not limited to, Qianjian trading Co., Ltd.

Preferably, the content of free phenol in the phenolic resin is 9-15%.

The source of the phenolic resin is not particularly limited, and there may be mentioned, but not limited to, commercial products of francisco, broccoli, ltd, model No. PF601, PF602, PF603, PF605, PF606, PF607, PF 608.

More preferably, the phenolic resin is purchased from commercial limited of Jinchuan, Jinhan, Jinan, model No. PF 605.

The inventor finds that after the phenolic resin, the polyethylene glycol and the citric acid are compounded for use, the polyethylene glycol with the component of more than 2000 is particularly selected, so that the liquid medicine has good viscosity, the suspended particles are prevented from settling, and a good anti-freezing effect is achieved. On the other hand, after the pesticide liquid is sprayed on crops, the pesticide liquid can be attached to the surfaces of the crops, so that the pesticide composition has longer effective period. The inventors believe that the possible reasons for this are that the phenolic resins all have good film forming properties and good binding to both polyethylene glycol and citric acid, especially with a free phenol content of around 10%. When the pesticide liquid prepared from the pesticide composition is sprayed on the surfaces of crops, the pesticide liquid can quickly form a film on the surfaces of the crops and tightly adhere to the crops, so that the influence of wind power and rain erosion on the crops is reduced, and the pesticide liquid can maintain a good and lasting insecticidal effect. In addition, the application of a certain amount of citric acid can play a role in regulating the pH value of the liquid medicine, and is beneficial to improving the efficacy of permethrin.

In the present invention, the disintegrating agent is not particularly limited, and sodium hydrogencarbonate, sodium carbonate, bentonite, urea, ammonium sulfate, aluminum chloride and the like can be mentioned.

Preferably, the disintegrant is ammonium sulfate.

Filler material

In the present invention, the filler is not particularly limited, and kaolin, white carbon, calcium carbonate, bentonite, kaolin, sericite powder and the like can be mentioned.

Preferably, the filler is white carbon black.

The third aspect of the invention provides a preparation method of the insecticidal pesticide water dispersible granule containing the diatomite with the special structure, which comprises the following steps:

The diatomite with the specific structure used in the invention has a sawtooth honeycomb structure, so the diatomite with the specific structure is added after the auxiliary agents are uniformly mixed, and the mechanical crushing cannot be used after the diatomite with the specific structure is added, so that the structure of the diatomite with the specific structure is prevented from being damaged.

When the used auxiliary agent contains liquid, the filler is firstly put into a container, the liquid auxiliary agent is added while stirring until the liquid auxiliary agent is completely adsorbed, then other auxiliary agents and the pesticide are added, and then the preparation method is carried out according to the steps.

The fourth aspect of the invention provides application of the insecticidal pesticide water dispersible granule containing the diatomite with the special structure, which can be applied to the control of red spiders, scale insects, frankliniella occidentalis, whiteflies, liriomyza sativae, prodenia litura, leafhoppers, thrips theae, tea geometrid, green plant bugs and grape aphids.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the starting materials used are all commercially available, unless otherwise specified.

Examples

Example 1

Embodiment 1 provides an insecticidal pesticide water dispersible granule containing diatomite with a special structure, which comprises 80% of an insecticidal pesticide composition, 15% of an auxiliary agent and 5% of a filler.

The insecticidal pesticide composition comprises diatomite and thiamethoxam with a zigzag honeycomb structure, and the weight ratio is 200: 1.

the diatomaceous earth having a saw-tooth honeycomb structure was purchased from Yirui Stone, France under the designation Celite 610.

The auxiliary agent comprises a dispersing agent, a wetting agent, a binder and a disintegrating agent, and the weight ratio is 1: 1: 1.2: 0.8.

the dispersant is sodium lignosulfonate; the wetting agent is sodium diisooctyl succinate sulfonate; the binder is phenolic resin, polyethylene glycol and citric acid, and the weight ratio of the binder to the binder is 1: 0.8: 1; the polyethylene glycol is polyethylene glycol 4000; the disintegrant is ammonium sulfate.

The filler is white carbon black.

The preparation method of the insecticidal pesticide water dispersible granule containing the diatomite with the special structure comprises the following steps:

(2) mixing the mixed material with diatomaceous earth with specific structure, shaking for 10min, and stirring for 30min to mix well to obtain mother powder;

Example 2

Example 2 differs from example 1 in that the pesticidal composition comprises diatomaceous earth having a saw-toothed honeycomb structure and clofentezine in a weight ratio of 50: 1.

example 3

Example 3 differs from example 1 in that the pesticidal composition includes diatomaceous earth having a saw-toothed honeycomb structure and permethrin in a weight ratio of 300: 1.

example 4

Example 4 differs from example 1 in that the pesticidal and pesticidal water dispersible granule comprises 70% of pesticidal and pesticidal composition, 25% of auxiliary agent and 5% of filler; the weight ratio of the dispersing agent to the wetting agent to the binder to the disintegrating agent is 1: 0.5: 0.8: 0.5; the weight ratio of the phenolic resin to the polyethylene glycol to the citric acid is 1: 0.3: 0.8.

example 5

Example 4 differs from example 1 in that the pesticidal and pesticidal water dispersible granule comprises 90% of pesticidal and pesticidal composition, 5% of auxiliary agent and 5% of filler; the weight ratio of the dispersing agent to the wetting agent to the binder to the disintegrating agent is 1: 1.2: 1.5: 1; the weight ratio of the phenolic resin to the polyethylene glycol to the citric acid is 1: 1: 1.5.

example 6

Example 6 differs from example 1 in that the dispersant is an aralkyl phenol polyoxyethylene ether phosphate.

Example 7

Example 7 differs from example 1 in that no dispersant is included.

Example 8

Example 8 differs from example 1 in that the wetting agent is nonylphenol polyoxyethylene ether.

Example 9

Example 9 differs from example 1 in that the binder does not contain polyethylene glycol.

Example 10

Example 10 differs from example 1 in that the polyethylene glycol has a molecular weight of 200.

Example 11

Example 11 differs from example 1 in that the phenolic resin is replaced by gelatin.

Performance testing

1. Measurement of suspension percentage: measured according to the method GB/T14825-2006.

2. And (3) measuring the wetting time: measured according to the method GB/T5451-2001.

The test results are shown in Table 1.

TABLE 1 test results of suspension percentage and wetting time of pesticide compositions provided in examples 1-11

3. Co-toxicity coefficient test (TS stands for Celite610)

The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect. The co-toxicity coefficient calculation formula is as follows:

the Theoretical Toxicity Index (TTI) of the mixture is the toxicity index of the agent A x the percent (%) of the agent A + the toxicity index of the agent B x the percent (%) of the agent B

(1) And (3) measuring the combined toxicity of the compounded TS and thiamethoxam on citrus red spiders.

Referring to the method in NY/T1154.13-2008 standard, a leaf spray method was used. Cleaning and wiping the smooth citrus leaves with the same size, sticking the citrus leaves onto wet sponge with the leaf surfaces facing upwards, enclosing the leaves with wet small cotton slivers to prevent test mites from escaping, putting the wet sponge into a culture dish with the diameter of 9cm, adding water into the culture dish, and slightly lowering the water level to be lower than the height of the sponge. Inoculating indoor cultured nymphs to leaves, each leaf having no less than 30 heads. Placing the culture dish on a bottom plate of a Potter spraying tower for spraying, wherein the spraying amount is 1mL, taking out the culture dish after the liquid medicine is settled for 1min, and transferring the culture dish to a breeding condition for breeding. Each treatment was repeated 4 times and a blank control was run.

After 48h, the death of the test insects was examined under a dissecting mirror and recorded. The judgment standard of the death of the test insects is as follows: the death was counted as no response when the test insects were lightly touched by tweezers.

TABLE 2 toxicity test results of compounding TS and thiamethoxam to citrus red spider

(2) And (3) determining the combined toxicity of the TS and thiamethoxam on the scale insects of citrus.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Dipping the trimmed citrus leaves with citrus arrowhead scales in a test solution for 10 seconds, taking out the citrus leaves, absorbing redundant liquid medicine around the leaves and the insects by using filter paper, putting the citrus leaves and the insects into a culture dish (with the diameter of 90mm) filled with moisturizing filter paper, wrapping petioles by using wet cotton balls for moisturizing, repeating the steps for 4 times per concentration, covering the dish cover after the treatment is finished, and placing the dish cover in an observation room for culturing.

After 72d, the death condition of the test insects is checked by using a dissecting mirror and recorded. The death judgment standard of the test insects is that the test insects are fresh and alive in color and are regarded as live insects in yellow or light yellow; the dead insects are considered to be dead insects if the body of the insects is shriveled and the color is dark.

TABLE 3 toxicity test results of compounding TS and thiamethoxam on Cucko orange (Cekurus)

(3) And (3) measuring the combined toxicity of the TS and thiamethoxam on Frankliniella occidentalis.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf-soaking dish method is adopted. Beating the blades into leaf disks by a puncher with the diameter of 18mm, soaking the leaf disks in a test reagent solution for 10 seconds, putting the leaf disks into a culture dish (with the diameter of 90mm) padded with moisturizing filter paper, putting 5 leaf disks into each dish, naturally drying, then selecting the western flower thrips into the culture dish, sealing the mouth of the dish by a preservative film, pricking some small holes on the film by a needle for ventilation, repeating the treatment for 4 times, finishing the treatment, and placing the dish in an observation room for culture.

The death of the test insects is checked after 72h and recorded. The standard for judging the death of the test insects is that the test insects do not react when the tip brush pen lightly contacts the test insects, and the death is counted.

TABLE 4 toxicity test results of TS and thiamethoxam combinations on Frankliniella occidentalis

(4) And (3) measuring the combined toxicity of the TS and thiamethoxam on the trialeurodes vaporariorum.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. After dissolving agar (1%) by heating, 20mL to 200mL of the solution was taken out of a beaker and condensed at room temperature for use. Soaking the leaves in a test solution for 10 seconds, taking out, naturally drying, sticking the leaves on the surface of the solidified agar solution, wrapping petioles with wet cotton balls, putting 3-4 leaves in each beaker, inoculating adult trialeurodes vaporariorum, covering 15 heads of each beaker with a preservative film, pricking small holes on the film with needles for ventilation, repeating the treatment for 4 times, and placing the beaker in an observation room for cultivation after the treatment.

The death of the test insects is checked after 72h and recorded. The standard for judging the death of the test insects is that no reaction is caused when the test insects are lightly contacted with tweezers, and the death is judged.

TABLE 5 toxicity test results of TS and thiamethoxam combinations against Bemisia alba

(5) And (3) determining the combined toxicity of the TS and the thiamethoxam to the liriomyza sativae.

Referring to the method in NY/T1154.13-2008 standard, spraying method is adopted. Transplanting potted cowpea pest-free seedlings into pest-raising cages, inoculating a certain amount of mated adult liriomyza huidonii, taking out bean seedlings with liriomyza huidonii eggs after 6h, placing the bean seedlings in another pest-raising cage, continuously raising the bean seedlings to 2-year larvae (ensuring that each disc contains no less than 80 larvae of 2-year larvae), then carrying out spraying treatment, naturally air drying, and transferring the bean seedlings to an artificial climate box for continuous raising. Each treatment was repeated 4 times and a blank control was run.

And (3) cutting bean seedlings from roots at the later stage of 3-year-old larvae, moisturizing the tail ends by using soaked cotton balls, putting the bean seedlings into plastic basins in an artificial climate box, pupating the bean seedlings, and counting the quantity of pupae in each dish.

TABLE 6 toxicity test results of the compounding of TS and thiamethoxam to Liriomyza sativae

(6) And (3) determining the combined toxicity of the compounded TS and thiamethoxam on prodenia litura.

Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted. Beat the cabbage leaf into the leaf dish with the hole puncher of diameter 18mm, dip the leaf dish in the liquid of testing medicine for 10 seconds after, put into the culture dish (diameter 90mm) that has filled with the filter paper that moisturizes, 6 leaf dishes are put into to every dish, wait to dry the back naturally, select again and starve 4h twill noctuid 3 age larva to the culture dish in, every dish is not less than 10, 4 times are repeated, the processing finishes, covers the ware lid, arranges the indoor culture of observation in.

The death of the test insects is checked after 72h and recorded. The standard for judging the death of the test insects is that no response is caused when the test insects are lightly contacted with tweezers, and the death is counted.

TABLE 7 toxicity test results of Twill and thiamethoxam compounding

(7) And (3) measuring the combined toxicity of the compounded TS and thiamethoxam on the tea leafhopper.

Referring to the method in NY/T1154.11-2008 standard, the dipping method is adopted. Selecting 3 fresh tea tree sprouts, soaking in a test liquid for 30 seconds, taking out, naturally drying, wrapping the base parts of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) with 4 times of repetition per concentration. Then, 3-year-old nymphs of tea lesser leafhoppers subjected to ether mild anesthesia treatment are inoculated into a brush pen, 15 +/-2 heads of each tube are used, and the mouth of each tube is sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

TABLE 8 toxicity test results of compounding TS and thiamethoxam on tea leafhopper

(8) And (3) measuring the combined toxicity of the TS and thiamethoxam on the tea leaf thrips tabaci.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf dipping method is adopted. Selecting fresh 3 tea tree sprout shoots with a certain amount of tea thrips (no less than 20) and soaking in the test liquid medicine for 10 seconds, taking out and naturally drying, wrapping the base parts of the sprout shoots with wet absorbent cotton to preserve moisture, sealing the absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) for 4 times of repetition at each concentration. The mouth of the test tube is sealed by a preservative film, and the small holes are punched for ventilation. After the treatment, the sample is placed in an observation chamber.

After 72h, the death condition of the test insects is checked under a dissecting mirror and recorded. The standard for judging the death of the test insects is that the test insects do not react when the tip brush pen lightly contacts the test insects, and the death is counted.

TABLE 9 toxicity test results of compounding TS and thiamethoxam on tea leaf thrips

(9) And (3) measuring the combined toxicity of the compounded TS and thiamethoxam on tea geometrid.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. Selecting 3 fresh tea tree sprouts, soaking in the test liquid medicine for 30 seconds, taking out, naturally drying, wrapping the bases of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a can bottle, wherein the concentration is repeated for 4 times. Then inoculating 2-year-old nymphs of tea geometrid with consistent size and liveness and health into a brush pen, wherein 15 +/-2 heads of each pot are sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

TABLE 10 toxicity test results of compounding TS and thiamethoxam to tea leaf geometrid

(10) And (3) measuring the combined toxicity of the compounded TS and thiamethoxam to the green plant bug.

Referring to the method in NY/T1154.11-2008 standard, a leaf spray method was used. The method comprises the steps of disinfecting, cleaning and airing fresh kidney beans by adopting an immersion method, cutting the kidney beans into 2-3 cm segments, immersing the kidney beans in liquid medicines with different concentrations for 30 seconds, airing the kidney beans under natural conditions, and then putting the kidney beans into transparent plastic boxes (6 cm in diameter and 7cm in height) with smooth air, wherein each box has two sections. Healthy and consistent 3-year-old nymphs of lygus lucorum were inoculated, 15 or so per box, repeated 4 times, and a blank control was set. And putting the plastic box into a light incubator for culturing.

After 48h, the death condition of the test insects in each box is checked, and the bodies of the lygus lucorum are lightly touched by the tip of the writing brush. The standard for judging the death of the test insects is that no response is caused when the test insects are lightly contacted with tweezers, and the death is counted.

TABLE 11 toxicity test results of compounding TS and thiamethoxam to green plant bug

(11) And (3) determining the combined toxicity of the compounded TS and thiamethoxam on grape aphids.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Cutting leaves with a certain amount of grape aphids, soaking in the liquid medicine to be tested for 10 seconds, taking out, absorbing the liquid medicine around the insect body with absorbent filter paper, placing in a culture dish padded with moisturizing filter paper, moisturizing with a wet cotton ball, and covering the culture dish with wet gauze. Each 30 aphids were treated, 4 replicates and a blank treatment without drug (including solvent and emulsifier used) was used. After the treatment, the cells were transferred to an observation room for cultivation.

After 48h, the death of the test insects is checked and recorded. The judgment standard of the death of the test insects is as follows: the aphids were lightly touched with a writing brush, and the body was judged dead.

TABLE 12 toxicity test results of compounding TS and thiamethoxam on grape aphid

(12) And (3) measuring the combined toxicity of the compounded TS and clofentezine to citrus red spiders.

TABLE 13 toxicity test results of Siberian mite and clofentezine combination on citrus red spiders

(13) And (3) measuring the combined toxicity of the compounded TS and permethrin to citrus red spiders.

After 72h, the death condition of the test insects is checked by using a dissecting mirror and recorded. The death judgment standard of the test insects is that the test insects are fresh and alive in color and are regarded as live insects in yellow or light yellow; the dead insects are considered to be dead insects if the body of the insects is shriveled and the color is dark.

TABLE 14 toxicity assay results for Packo citrus (arrowhead scale) paired with TS and permethrin

(14) And (3) measuring the combined toxicity of the TS and permethrin on the Frankliniella occidentalis.

TABLE 15 toxicity test results of TS and permethrin combinations on Frankliniella occidentalis

(15) And (3) determining the combined toxicity of the TS and permethrin on the trialeurodes vaporariorum.

TABLE 16 toxicity test results of TS and permethrin combinations against Bemisia alba

(16) And (3) determining the combined toxicity of the TS and the permethrin to the liriomyza sativae.

TABLE 17 toxicity test results of compounding TS and permethrin against Liriomyza sativae

(17) And (3) determining the combined toxicity of the TS and permethrin on prodenia litura.

The death of the test insects is checked after 72h and recorded. The standard for judging the death of the test insects is that no response is calculated when the tweezers lightly contact the test insects.

TABLE 18 toxicity test results of Twill combined with permethrin

(18) And (3) determining the combined toxicity of the TS and permethrin on the tea leafhoppers.

TABLE 19 toxicity test results of compounding of TS and permethrin on tea leafhoppers

(19) And (3) determining the combined toxicity of the TS and permethrin on the tea leaf thrips tabaci.

TABLE 20 toxicity test results of the compounding of TS and permethrin on tea leaf thrips tabaci

(20) And (3) measuring the combined toxicity of the TS and permethrin on tea geometrid.

TABLE 21 toxicity test results of compounding TS and permethrin on tea geometrid

(21) And (4) performing combined toxicity determination on the green plant bug by the TS and permethrin.

And after 48h, the death condition of the test insects in each box is detected, the bodies of the lygus lucorum are lightly touched by the brush tips, and the death judgment standard of the test insects is that no reaction is caused when the test insects are lightly touched by tweezers, namely the death is counted.

Table 22 toxicity test results of compounding TS and permethrin on green plant bug

(22) And (3) determining the combined toxicity of the compounded TS and permethrin on grape aphids.

TABLE 23 toxicity test results of TS and permethrin combinations on grape aphids

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may modify or change the technical content disclosed above into an equivalent embodiment with equivalent changes, but all those simple modifications, equivalent changes and modifications made on the above embodiment according to the technical spirit of the present invention still belong to the protection scope of the present invention.

Claims

1. An insecticidal pesticide composition containing diatomite with a special structure is characterized by comprising the diatomite with the special structure and an insecticide in a weight ratio of 1: (0.0005-50); the diatomite with the specific structure has a sawtooth-shaped honeycomb structure.

2. An insecticidal and pesticidal composition according to claim 1, wherein said insecticide is any one of thiamethoxam, clofentezine and permethrin.

3. An insecticidal and pesticidal composition according to claim 2, wherein the weight ratio of the specific structure diatomaceous earth to thiamethoxam is 1: 40-100: 1.

4. an insecticidal and pesticidal composition according to claim 2, wherein the weight ratio of the specific structure diatomaceous earth to clofentezine is 1: 40-100: 1.

5. an insecticidal and pesticidal composition according to claim 2, wherein the weight ratio of the specific structure diatomaceous earth to permethrin is 1: 40-600: 1.

6. an insecticidal and pesticidal composition according to any one of claims 1 to 5, wherein the formulation of the insecticidal and pesticidal composition is selected from any one of wettable powder, water dispersible granule, suspension, emulsifiable concentrate, aqueous emulsion, powder, granule, effervescent tablet, dispersible tablet.

7. An insecticidal pesticide water dispersible granule containing diatomite with a special structure is characterized in that the preparation raw materials comprise any one of 1-5 insecticidal pesticide compositions; the pesticide composition comprises 70-90% of pesticide composition, 5-25% of auxiliary agent and 100% of filler in percentage by weight; the auxiliary agent comprises at least one of a dispersing agent, a wetting agent, a stabilizing agent, a disintegrating agent, a separating agent, a stabilizing agent and a binder.

8. An insecticidal pesticide water dispersible granule according to any one of claim 8, wherein the binder comprises a combination of phenolic resin, polyethylene glycol and citric acid in a weight ratio of 1: (0.3-1): (0.8-1.5).

9. A method for preparing the pesticidal and pesticidal water dispersible granule according to any one of claims 7 to 8, characterized by comprising the steps of:

(2) mixing the mixed material with diatomaceous earth with specific structure, shaking for 5-20min, and stirring for 20-400min to mix well to obtain mother powder;

10. The use of the pesticidal and pesticidal water dispersible granule according to any one of claims 7 to 8, which is applicable to the control of red spiders, scale insects, frankliniella occidentalis, whiteflies, liriomyza sativae, prodenia litura, leafhoppers, thrips theae, ectropis obliqua, lygus lucorum and grape aphids.