CN116077410A

CN116077410A - Composition and preparation with skin barrier repairing function, and preparation and application thereof

Info

Publication number: CN116077410A
Application number: CN202310027239.7A
Authority: CN
Inventors: 杨积胜; 林泽森; 罗嘉敏; 陈雅雯; 陈结莹
Original assignee: Guangzhou Yanduo Cosmetics Technology Co ltd
Current assignee: Guangzhou Yanduo Cosmetics Technology Co ltd
Priority date: 2023-01-09
Filing date: 2023-01-09
Publication date: 2023-05-09

Abstract

The invention discloses a composition and a preparation with skin barrier repairing function, and preparation and application thereof, and belongs to the technical field of cosmetics. Selecting crocus sativus extract, deeply repairing and stabilizing, and stabilizing inflammatory skin; oat beta-glucan promotes the generation of moisturizing factors, promotes endogenous moisturizing and enhances barrier function; sodium mannose phosphate is involved in metabolism of substances and energy; ceramide rebuilds and stabilizes the skin barrier; silk fibroin promotes cell attachment, directs cell migration. The tetrahydropyrimidine carboxylic acid maintains the microecological balance of the skin surface. And (3) wrapping the raw materials with hydrogenated lecithin to form lecithin liposome. The cosmetic which is used as the additive or the basic raw material has the functions of improving skin discomfort, repairing skin barrier and the like, and the product is a milky viscous paste wrapped by hydrogenated lecithin, is convenient to transport and store, is beneficial to promoting the slow release, continuous permeation, reduction of irritation and improvement of using effect of the active ingredients when the product is used.

Description

Composition and preparation with skin barrier repairing function, and preparation and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a skin barrier repairing function, a preparation and application thereof.

Background

Human skin acts as an active immune organ, and in the basic state of tolerogenicity (non-inflammatory), the skin contains a rich variety of immune cells including macrophages, mast cells, natural killer cells, lymphocytes, T cells and a large number of DCs. Once the skin's own system detects the "danger" signal, it transitions from tolerability to an immunogenic state. LCs are activated and migrate to the dermis or lymph nodes to activate effector T cell responses. Activation of dendritic cells in the dermis is also increased, while cytokines are produced that directly activate specific polarity T cell subsets. Wherein inflammatory mediators slow the rate of skin repair; the presence of inflammatory factors promotes the rate of melanin production by melanocytes, further exacerbating pigmentation; inflammatory mediators promote the release of MMPs, decompose macromolecular proteins, and collapse and age skin; inflammatory mediators slow down the repair speed of skin lesions and also affect the balance of micro-ecology, which leads to excessive multiplication of harmful bacteria and the skin problems of acne and white head.

Skin barrier dysfunction is a typical feature of Atopic Dermatitis (AD), and many phenotypes of sensitive muscles are similar to AD. Such as loose keratinous barrier structure, itching, redness, and the like. Inflammatory factors that are primarily present in AD include IL-4, IL-5, IL-13, IL-31, and the like. IL-4 and IL-13 not only down regulate FLG expression and promote colonisation of the skin surface by Staphylococcus aureus, but also act directly on neurons to promote the occurrence of itch.

At present, a new term of mask face is also generated due to the fact that the mask is worn for a long time. The "mask face" is generally a generic term for facial skin problems caused by wearing the mask for a long time, such as skin inflammation symptoms including allergic redness, pressure injury, acne, oil-producing acne, etc. However, the main causes of these skin problems are: 1. is airtight for a long time; 2. friction and pressure; 3. a microbial infection; 4. mask material allergy, etc.

Skin problems arising from these causes are therefore currently a psychological burden for the vast majority of sensitive muscle masses.

Aiming at the current situation, most products on the market are mainly improved in a single aspect, the existing skin problems are generally solved, and the effects of the products are slower in permeation and absorption, single in function and poor in repairing effect.

Disclosure of Invention

The invention aims to provide a composition with skin barrier repairing function, wherein the composition can strengthen skin barriers in a manner of respectively controlling inflammation, preserving moisture and increasing cutin of cells in skin by selecting various raw materials for combination; the cell matrix and collagen are supplemented to the external cells of the skin, so that the connection performance among the cells is enhanced, and the skin barrier is stabilized. Solves the technical problem that the repairing effect is poor because the existing product can only start from skin epidermis cells.

The invention is realized by the following technical scheme:

the composition with the skin barrier repairing function is mainly prepared from the following raw materials in parts by mass:

5-10 parts of component A, 1-5 parts of component B, 0.1-0.5 part of component C, 1-5 parts of component D, 0.5-2 parts of component E, 0.1-0.5 part of component F and 0.01-0.1 part of component G;

the component A consists of 83wt% of water, 15wt% of 1, 3-propanediol and 2wt% of crocus sativus extract;

the component B consists of 10wt% of oat beta-glucan and 90wt% of erythritol;

the component C is 100wt% of ceramide NP;

the component D is 100wt% of hydrogenated lecithin;

the component E consists of 3.4wt% of sodium mannose phosphate, 1.5wt% of mannose, 50wt% of glycerol and 45.1wt% of water;

the component F is 100wt% of tetrahydropyrimidine carboxylic acid;

the component G consists of 98wt% of fibroin, 1.8wt% of disodium hydrogen phosphate and 0.2wt% of sodium dihydrogen phosphate.

In the composition with skin barrier repairing function, CROCUS SATIVUS flower extract is selected, so that deep repairing and maintenance are stable, inflammatory skin is stabilized, inflammatory related channels and main inflammatory factors are inhibited, the concentration of inflammatory factor TNFα can be remarkably reduced, the opening of NF-KB signal channels is avoided, pain receptor TRPV1 is antagonized, and skin sense discomfort is reduced. Under the condition of controlling skin inflammation, the skin epidermis barrier needs to be repaired, so that small molecular oat beta-glucan is matched, FLG (filaggrin) expression is promoted, caspase-14 expression is promoted, and then the generation of moisturizing factor NMF is promoted, endogenous moisturizing is promoted, and the barrier function is enhanced; and secondly, regulating and controlling a calcium ion signal channel, promoting normal differentiation of keratinocytes, accelerating barrier formation and promoting barrier repair. However, the energy consumption in the process of internal cell repair is very huge, so that the reduction of the energy consumption can promote the progress of normal cell metabolism, and the mannose sodium phosphate can be used for participating in the metabolism of substances and energy in mitochondria of animal cells, compared with the normal glycolysis pathway initiated by glucose, the mannose has one phosphate bond, so that 1 molecule of ATP can be consumed less, and the energy consumption is reduced.

After solving the problems of factors such as cells in the skin, it is also necessary to externally supplement the major components in intercellular lipids: and (3) ceramide. The cell matrix can prevent abnormal loss of in vivo water and electrolyte, prevent harmful substances from entering, strengthen keratinocyte links, and rebuild and stabilize skin barrier. In the skin where wounds are already formed, the silk fibroin can inhibit the expression of alpha-SMA genes, further inhibit the expression of type I collagen, reduce disordered deposition of collagen, optimize the arrangement and density of collagen fibers, and further achieve the effects of scar fading and natural skin tissue structure reconstruction. And the fiber structure in the gel and film (especially electrostatic spinning nanometer fiber film) formed by silk fibroin when meeting water is similar to the extracellular matrix formed by collagen in skin, thus playing the roles of promoting cell attachment and guiding cell migration.

Finally, the micro-ecological balance on the surface of the skin can be maintained by selecting and matching with the tetrahydropyrimidine carboxylic acid, so that the normal pH level of the skin can be maintained, the expression of the antibacterial peptide can be promoted, and the substances of the human body or the environment can be regulated for organism immunity and catabolism. Forming the outermost protective barrier of our skin.

Because the selected small molecular oat beta-glucan, CROCUS SATIVUS flower extract and other raw materials are water-soluble raw materials, the compatibility with skin is relatively poor, hydrogenated lecithin components are added on the basis, the raw materials are coated with hydrogenated lecithin, and the lecithin liposome is applied to the product, so that the effects of promoting permeation and reducing irritation are achieved.

Preferably, the composition is mainly prepared from the following raw materials in parts by mass:

6-8 parts of component A, 2-4 parts of component B, 0.2-0.4 part of component C, 2-4 parts of component D, 1-1.5 parts of component E, 0.2-0.4 part of component F and 0.05-0.08 part of component G.

Preferably, the preparation method of the component A comprises the following steps: strictly selecting crocus sativus pistils, treating the crocus sativus pistils by utilizing enzymatic hydrolysis, and filtering to obtain crocus sativus extracts;

adding 1, 3-propylene glycol and water into the crocus sativus extract, and mixing to obtain component A.

The flowering period of the selected crocus sativus is 10 months. Because flowers are very delicate, all picking is done manually, rather than using a machine, to ensure the quality of the flowers.

The enzyme hydrolysis process belongs to the field of high specificity and mild condition. The configuration of the glycosidic bond can be obtained by enzymatic hydrolysis of the glycosidic bond, the structure of the aglycone is kept unchanged, and a part of the glycosidic bond is kept to obtain secondary glycoside or oligosaccharide. Among the bitter components of crocus sativus, crocin is the most predominant component, and is hydrolyzed by acid to obtain glucose and crocin, and hydrolyzed by enzyme to obtain crocin oxide.

Preferably, the preparation method of the component B comprises the following steps: washing wheat with second-stage reverse osmosis water, performing enzymolysis, extracting and separating to obtain oat beta-glucan, adding erythritol, stirring for dissolving, sterilizing, and freeze-drying to obtain component B for later use.

The secondary reverse osmosis water is selected because the ion content is particularly low, so that the influence of impurities in the water on wheat is eliminated.

Preferably, the preparation method of the component E comprises the following steps: mannose, sodium hexametaphosphate and hexokinase react under a system of aqueous solution to generate mannose sodium phosphate, and after sterilizing and purifying the compound glycerol, the component E is obtained for standby.

The ceramide NP in the component C is prepared by sequentially adding phytosphingosine and ethyl oleate into a reaction kettle according to a certain proportion, continuously reacting in a water bath kept at 80-90 ℃ at constant temperature, stirring after the reaction is finished, cooling after crystal precipitation, filtering the obtained reaction liquid to obtain a filter cake, and drying under reduced pressure.

The component D is obtained by hydrogenation purification of soybean phospholipid.

The component F is prepared from raw materials of Sanshen Kadsura biological technology Co.

The fibroin of the component G is selected from raw materials produced by China center-initiated biotechnology (Hangzhou) limited company.

A method of preparing a composition having skin barrier repair function comprising:

and (3) putting the component D and the component C into a reaction kettle, uniformly mixing, adding the component A, the component E, the component F and the component G, heating, stirring, adding the component B, fully stirring, uniformly mixing, starting a high-pressure homogenizing device, and wrapping the components by lecithin through the high-pressure homogenizing device to finish the preparation.

Preferably, the composition is a milky viscous paste.

Preferably, the temperature is raised to 80-85 ℃;

the heating and stirring time is 30-60min.

A formulation having skin barrier repair function comprising the composition having skin barrier repair function.

The application of the preparation with the skin barrier repairing function adopts the preparation to prepare water, milk, cream, essence and mask products for skin barrier repairing in cosmetics as additives;

the adding proportion is 0.1wt percent to 5wt percent.

Compared with the prior art, the invention has at least the following technical effects:

the invention provides a composition with skin barrier repairing function, which is prepared from crocus sativus flower extract, has effects of deeply repairing and stabilizing skin, stabilizing inflammatory skin, inhibiting inflammatory related pathways and main inflammatory factors, remarkably reducing the concentration of inflammatory factor TNFa, avoiding the opening of NF-KB signal pathway, antagonizing pain receptor TRPV1, and reducing skin sense discomfort. Under the condition of controlling skin inflammation, the skin epidermis barrier needs to be repaired, so that small molecular oat beta-glucan is matched, FLG (filaggrin) expression is promoted, caspase-14 expression is promoted, and then the generation of moisturizing factor NMF is promoted, endogenous moisturizing is promoted, and the barrier function is enhanced; and secondly, regulating and controlling a calcium ion signal channel, promoting normal differentiation of keratinocytes, accelerating barrier formation and promoting barrier repair. However, the energy consumption in the process of internal cell repair is very huge, so that the reduction of the energy consumption can promote the progress of normal cell metabolism, and the mannose sodium phosphate can be used for participating in the metabolism of substances and energy in mitochondria of animal cells, compared with the normal glycolysis pathway initiated by glucose, the mannose has one phosphate bond, so that 1 molecule of ATP can be consumed less, and the energy consumption is reduced.

And secondly, the cosmetics which are applied by taking the composition as an additive or a basic raw material have the functions of improving skin discomfort, repairing skin barrier and the like, and have safety and no side effect.

The preparation prepared from the composition is a milky viscous paste wrapped by hydrogenated lecithin, so that the stability of the transportation and storage performances of the product is ensured, the slow release of active ingredients is facilitated when the product is used, the continuous permeation of the product is ensured on the premise of ensuring the effective service life of the product, the stimulation is reduced, and the use effect is improved.

Drawings

FIG. 1 is a graph showing the trend of the moisture content of the skin horny layer in a test area of a test subject;

FIG. 2 is a graph showing the trend of the repair rate of each sample on the moisture content of the barrier damaged skin stratum corneum according to the experimental example;

FIG. 3 is a graph showing the variation trend of TEWL in the test area of the experimental subject;

fig. 4 is a graph showing the repair rate trend of each sample of the experimental example on the barrier damaged skin TEWL.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the following examples, which are to be construed as merely illustrative and not limitative of the scope of the invention, but are not intended to limit the scope of the invention to the specific conditions set forth in the examples, either as conventional or manufacturer-suggested, nor are reagents or apparatus employed to identify manufacturers as conventional products available for commercial purchase.

The specific components are as follows:

the component B consists of 10wt% of oat beta-glucan and 90wt% of erythritol;

the component C is 100wt% of ceramide NP;

the component D is 100wt% of hydrogenated lecithin;

the component F is 100wt% of tetrahydropyrimidine carboxylic acid;

Example 1:

500G of component A, 100G of component B, 10G of component C, 100G of component D, 50G of component E, 100G of component F and 1G of component G.

and (3) putting the component D and the component C into a reaction kettle, uniformly mixing, adding the component A, the component E, the component F and the component G, heating to 80 ℃, stirring for 30min, adding the component B, fully stirring and uniformly mixing, starting a high-pressure homogenizing device, and coating the components with lecithin through the high-pressure homogenizing device to finish the preparation.

Example 2:

1000 grams of component A, 500 grams of component B, 50 grams of component C, 500 grams of component D, 200 grams of component E, 50 grams of component F and 10 grams of component G.

and (3) putting the component D and the component C into a reaction kettle, uniformly mixing, adding the component A, the component E, the component F and the component G, heating to 85 ℃, stirring for 60min, adding the component B, fully stirring and uniformly mixing, starting a high-pressure homogenizing device, and coating the components with lecithin through the high-pressure homogenizing device to finish the preparation.

Example 3:

600 grams of component A, 200 grams of component B, 20 grams of component C, 200 grams of component D, 100 grams of component E, 20 grams of component F and 5 grams of component G.

and (3) putting the component D and the component C into a reaction kettle, uniformly mixing, adding the component A, the component E, the component F and the component G, heating to 85 ℃, stirring for 30min, adding the component B, fully stirring and uniformly mixing, starting a high-pressure homogenizing device, and coating the components with lecithin through the high-pressure homogenizing device to finish the preparation.

Example 4:

800G of component A, 400G of component B, 40G of component C, 400G of component D, 150G of component E, 40G of component F and 8G of component G.

and (3) putting the component D and the component C into a reaction kettle, uniformly mixing, adding the component A, the component E, the component F and the component G, heating to 80 ℃, stirring for 60min, adding the component B, fully stirring and uniformly mixing, starting a high-pressure homogenizing device, and coating the components with lecithin through the high-pressure homogenizing device to finish the preparation.

Test example:

the test sample was selected for verification in example 2.

1. Principle of testing

The test takes a clinical test as a theoretical basis, mainly adopts subjective evaluation and objective skin index evaluation to analyze the improvement effect of the skin state of a subject before and after use, and the result is more visual, thereby being applicable to the efficacy evaluation of finished cosmetics or semi-finished cosmetics.

Based on clinical medicine, a tested group is formed by specific groups, a skin barrier damage model is formed by peeling an adhesive tape, and the change of physiological indexes such as moisture content of skin horny layer, percutaneous moisture loss rate (TEWL) and the like before and after the skin barrier damage model is tested by using cosmetics is measured, so that the efficacy of the cosmetics is evaluated.

2. Materials and instruments

2.1 test article

Note that: the table is replaced by "/" for the empty space

The using method of the product comprises the following steps: the product was used on the curved side of the arm in the area stripped by the tape, 1 time a day, and the area stripped by the tape without the product was set to about 2mg/cm air as a model control.

2.2 instruments

Stratum corneum moisture content tester (Corneometer CM825, germany);

percutaneous Water loss Rate tester (Delfin, vapometer, finland).

3. Test method

3.1 subject

According to the declaration of helsinki, the choice of the subject follows the medical and ethical criteria of the human test, all subjects' tests must be voluntary by the subject himself, and an informed consent is signed before the test. The subject needs to be informed by the tester of the purpose, possible benefits, potential risks and problems of the test, and the associated rights and obligations of the test prior to signing the informed consent.

3.1.1 number of subjects target number of subjects: 30 people

3.1.2 inclusion criteria:

1) Age 18-65 years;

2) The skin on the curved side of the forearm is healthy, the skin color is uniform, and the testing conditions are not affected by scars and the like;

3) Can be returned and subjected to skin test as required.

3.1.3 exclusion criteria:

1) During pregnancy or lactation;

2) The skin care product and the washing and caring product have allergy history, and are allergic to certain components in the test product and the matched sample;

3) Patients with severe systemic diseases and other skin diseases, such as lupus erythematosus, psoriasis, etc.;

4) The test area received cosmetic therapy;

5) Other clinical trial studies have been conducted either currently or prior to the initiation of the study.

3.1.4 exit (drop) criteria:

1) Subjects were out of visit and actively asked to exit;

2) The compliance is poor, the samples can not be used in time according to the quantity, and the return visit can not be performed according to the requirement;

3) Other cosmetics similar to the test samples were used during the study;

4) New diseases, including skin diseases, appear throughout the study period that directly affect clinical status assessment;

5) Patients with severe disease appear throughout the study period;

6) Pregnant subjects during the study;

7) Intolerance to the present investigator:

8) Severe adverse reactions occurred throughout the study period.

3.2 test index

3.2.1 moisture content of the stratum corneum of the skin

Skin stratum corneum moisture content was tested by the skin moisture content test probe (ComeometerCM 825) of the multifunctional skin tester MPA 580, CK, germany. The probe measures the moisture content of the surface layer of the skin by a capacitance method. The permittivity of water in the skin surface is far higher than that of other substances, the change of the permittivity of the skin surface is mainly caused by the change of the moisture content of the skin surface, and the moisture content of the skin surface can be analyzed by measuring the permittivity of the skin surface. The measurement is a relative value, expressed in units of c.u. (Corneometer Units). The higher the number, the higher the moisture content of the skin surface.

3.2.2 percutaneous Water loss Rate (TEWL)

Percutaneous water lossThe rate (Transepidermal water loss, TEWL) was tested by a moisture loss rate tester (vaponter) from Delfin corporation, finland. The top of the tester is provided with a circular opening, a closed cavity is formed after the tester contacts skin, and a high-precision humidity sensor in the cavity records the change condition of the air humidity in the cavity. The rate of moisture loss through the skin can be calculated by the rate of change of air humidity over time over a specified period of time after contact with the skin. The unit of measurement is g/(m) ² H) the lower the value, the lower the rate of water loss from the skin surface, the better the skin barrier function.

3.3 measuring position

3 regions of 3cm by 3cm were selected as test regions on the curved side of the arm, each region being spaced at least 1cm apart, and each region being peeled off 5 times per day on days 0 to 2 for 3 consecutive days. According to the random table, 2 regions were used as sample areas 1 product per day, and another 1 region was used as model control without product.

3.4 test Environment

The skin of the subject was exposed to the skin on the curved side of the arm, and skin measurement was performed after balancing for 20 minutes in an environment with a temperature of 20-22 ℃ and a humidity of 40-60%.

3.5 test flow

(1) On day 0, subjects received screening after signing informed consent, and qualified subjects were screened into groups. Marking 3 areas with the thickness of 3cm multiplied by 3cm on the curved side of the arm as a test area, balancing the constant temperature and constant humidity environment for 20 minutes, measuring the moisture content and TEWL of the skin cuticle of the test area, and stripping the skin cuticle of the test area by using an adhesive tape for 5 times; the product was then applied at the sample area at about 2mg/cm ² The model control area uses no product.

(2) Stripping the test area with adhesive tape every day for 5 times on days 1-2; the product was then applied at the sample area at about 2mg/cm ² The model control area uses no product.

(3) On days 3-6, about 2mg/cm of product was applied daily to the sample area ² The model control area uses no product.

(4) On day 7, the skin stratum corneum moisture content, TEWL, of the test area was measured after 20 minutes of equilibration in a constant temperature and humidity environment.

3.6 adverse reactions

The volunteers should immediately stop using the test article in any adverse reaction, and go to the study center for examination by the physician and judge if to terminate the sample use according to the situation. Any adverse reactions and other related events that occur during the test should be recorded and reflected in the report.

3.7 data analysis

The mean and standard deviation of all subjects at each time point for each test index were calculated. The change value and the change rate with respect to day 0 are calculated as follows:

variation value (delta value) =t _n -T ₀ ；

Rate of change (%) = (T _n -T ₀ )/T ₀ *100％

Wherein T is ₀ Measurement average of measurement index on day 0

T _n Average value of the measurement index on the nth day

n-date of return visit

The repair proportion of the test sample is calculated, and the calculation formula is as follows:

repair rate = (Rc-Rs)/Rc 100%

Wherein, the Rc-model controls the skin physiological index change rate of the area;

rs-rate of change of skin physiological index of test sample region

And (3) carrying out statistical analysis on the data set by using SPSS 22.0 software, analyzing by adopting paired t test if the data are normally distributed, and analyzing by adopting rank sum test if the data are not normally distributed, wherein the data are both two-tail test, and the test level alpha=0.05.

4. Test results

The trial recruited 32 eligible subjects and completed the test at day 29 to day 5 of month 29 of 2022. Of the 32 subjects, 3 men and 29 women were aged 24-58 years with an average age of 41.2.+ -. 10.7 years. Adverse reactions were not reported during the test. The results of the measurement of the moisture content of the skin horny layer and the percutaneous moisture loss rate are as follows.

4.1 moisture content of the stratum corneum of the skin

Skin stratum corneum moisture content was tested by the skin moisture content test probe (corneometer cm 825) of the germany CK company multifunctional skin tester MPA 580. The probe measures the moisture content of the surface layer of the skin by a capacitance method. The permittivity of water in the skin surface is far higher than that of other substances, the change of the permittivity of the skin surface is mainly caused by the change of the moisture content of the skin surface, and the moisture content of the skin surface can be analyzed by measuring the permittivity of the skin surface. The measurement is a relative value, expressed in units of c.u. (Corneometer Units). The higher the number, the higher the moisture content of the skin surface.

Descriptive statistics of skin stratum corneum moisture content during the test are shown in table 1:

TABLE 1 statistical results of description of moisture content of skin horny layer

Note that: comparison with model control p<0.05,**p<0.01 _o

As shown in fig. 1, a graph of the moisture content change trend of the skin horny layer in the test area of the subject is shown.

As shown in fig. 2, a graph of the repair rate trend of each sample against moisture content of barrier damaged skin horny layer is shown.

The above results show that: the tape was peeled off for 3 consecutive days and 1 product was used daily until day 7:

the average skin stratum corneum moisture content in the model control area was reduced by about 9.13 compared to day 0;

the average values of the moisture content of the skin horny layer in the areas of the test samples "model control group", "Base", "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" were reduced by about 9.13, 7.71, 3.94, 6.54, 5.95, 3.34 respectively compared with the day 0;

the use of test samples "Base", "Base + sample 1", "Base + sample 2", "Base + sample 3", "Base + sample 4" reduced the rate of reduction of the moisture content of the skin stratum corneum by about 16.6%, 56.4%, 26.1%, 36.5%, 62.8%, respectively.

The measured value of the moisture content of the stratum corneum of the area of the test sample 'Base' is not significantly different from that of the control area (p is more than or equal to 0.05), and the measured value of the moisture content of the stratum corneum of the area of the test sample 'base+ sample 1', 'base+ sample 2', 'base+ sample 3', 'base+ sample 4' is significantly higher than that of the control area (p < 0.05);

the increase in the moisture content of the stratum corneum in the "Base" region of the test sample was not significantly different from that in the control region (p.gtoreq.o.05), and the increase in the moisture content of the stratum corneum in the "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" region of the test sample was significantly higher than that in the control region (p < 0.05).

4.2 percutaneous Water loss Rate (TEWL)

The percutaneous water loss rate (Transepidermal water loss, TEWL) was tested by a water loss rate tester (vaponter) from Delfin corporation, finland. The top of the tester is provided with a circular opening, a closed cavity is formed after the tester contacts skin, and a high-precision humidity sensor in the cavity records the change condition of the air humidity in the cavity. The rate of moisture loss through the skin can be calculated by the rate of change of air humidity over time over a specified period of time after contact with the skin. The unit of measurement is g/(m) ² H) the lower the number, the lower the rate of water loss from the skin surface, the better the skin barrier function. Descriptive statistics of TEWL during the test are shown in table 2:

TABLE 2 descriptive statistics of TEWL

Sequence number

Name of the name

Day

0

Day 7

Change value

Repair rate

0

Model control group

4.82±1.19

7.96±1.05

3.14±0.96

0.0％

1

Base

4.92±1.54

7.54±1.81

2.63±1.91

18％

2

Base+ sample 1

4.69±1.21

6.90±1.40**

2.22±1.46**

27.5％

3

Base+ sample 2

4.78±1.59

7.29±1.16*

2.51±1.86*

19.4％

4

Base+ sample 3

4.86±1.12

7.28±1.45*

2.42±1.61*

23.6％

5

Base+ sample 4

4.98±1.64

7.03±1.34**

2.05±1.77**

36.8％

Note that: p <0.05, < p <0.01, in comparison to model control.

As shown in fig. 3, a TEWL trend graph is shown for the test area of the subject.

As shown in fig. 4, a graph of how each sample was subjected to repair rate of barrier damaged skin TEWL.

The above results indicate that: the tape was peeled off for 3 consecutive days and 1 product was used daily until day 7: the mean TEWL for the model control area increased by about 3.14 compared to day 0;

the average skin TEWL for the "model control", "Base + sample 1", "Base + sample 2", "Base + sample 3", "Base + sample 4" areas using the test samples were raised by about 3.14, 2.63, 2.22, 2.51, 2.42, 2.05, respectively, compared to day 0;

the use of test samples "Base", "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" reduced the rate of skin TEWL elevation by about 18.0%, 27.5%, 19.4%, 23.6%, 36.8%, respectively.

The TEWL measurement values of the areas of the test sample 'Base' and the control areas are not significantly different (p is more than or equal to 0.05), and the TEWL measurement values of the areas of the test sample 'base+sample 1', 'base+sample 2', 'base+sample 3', 'base+sample 4' are significantly lower than the control areas (p < 0.05);

the TEWL increase for the "Base" region of the test sample was not significantly different from the control region (p.gtoreq.0.05), and the TEWL increase for the "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" region of the test sample was significantly lower than the control region (p < 0.05).

Conclusion of the test:

1. the trial recruited 32 eligible subjects and completed the test at day 29 to day 5 of month 29 of 2022. Of the 32 subjects, 3 men and 29 women were aged 24-58 years with an average age of 41.2.+ -. 10.7 years. The skin barrier was slightly damaged by tape stripping 5 times per day on the curved side of the subject's arm for 3 consecutive days, 1 product was quantitatively applied to the laboratory per day, and the area stripped by tape but not applied with product was set as a model control, and the skin horny layer moisture content and TEWL of the test area were measured before and at day 0 and at day 7, respectively. Adverse reactions were not reported during the trial, and 32 subjects completed the test. The test results of this test are as follows: the tape was peeled off for 3 consecutive days and 1 product was used daily until day 7: the average skin moisture content of the model control area was reduced by about 9.13 compared to day 0, and the average skin moisture content of the test samples "Base", "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" areas was reduced by about 7.71, 3.94, 6.54, 5.95, 3.34 respectively compared to day 0; the use of test samples "Base", "Base + sample 1", "Base + sample 2", "Base + sample 3", "Base + sample 4" reduced the rate of reduction of the moisture content of the skin stratum corneum by about 16.6%, 56.4%, 26.1%, 36.5%, 62.8%, respectively. The measured value of the moisture content of the stratum corneum of the area of the test sample 'Base' is not significantly different from that of the control area (p is more than or equal to 0.05), and the measured value of the moisture content of the stratum corneum of the area of the test sample 'base+ sample 1', 'base+ sample 2', 'base+ sample 3', 'base+ sample 4' is significantly higher than that of the control area (p < 0.05); the increase in the moisture content of the stratum corneum in the "Base" region of the test sample was not significantly different from that in the control region (p.gtoreq.0.05), and the increase in the moisture content of the stratum corneum in the "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" region of the test sample was significantly higher than that in the control region (p < 0.05).

2. The tape was peeled off for 3 consecutive days and 1 product was used daily until day 7: the average TEWL for the model control area increased by about 3.14 compared to day 0, and the average TEWL for the skin using the test samples "Base", "Base + sample 1", "Base + sample 2", "Base + sample 3", "Base + sample 4" areas increased by about 2.63, 2.22, 2.51, 2.42, 2.05 respectively compared to day 0; the use of test samples "Base", "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" reduced the rate of skin TEWL elevation by about 18.0%, 27.5%, 19.4%, 23.6%, 36.8%, respectively. The TEWL measurements using the test sample "Base" region were not significantly different from the control region (p.gtoreq.o.05), the TEWL measurements using the test sample "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" region were significantly lower than the control region (p < 0.05); the TEWL increase for the "Base" region of the test sample was not significantly different from the control region (p.gtoreq.0.05), and the TEWL increase for the "base+sample 1", "base+sample 2", "base+sample 3", "base+sample 4" region of the test sample was significantly lower than the control region (p < 0.05).

Finally, it should be noted that: the foregoing description is only of the preferred embodiments of the invention and is not intended to limit the scope of the invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The composition with the skin barrier repairing function is characterized by being prepared from the following raw materials in parts by weight:

the component B consists of 10wt% of oat beta-glucan and 90wt% of erythritol;

the component C is 100wt% of ceramide NP;

the component D is 100wt% of hydrogenated lecithin;

the component F is 100wt% of tetrahydropyrimidine carboxylic acid;

2. The composition with skin barrier repair function according to claim 1, wherein the composition is mainly prepared from the following raw materials in parts by mass:

3. A composition with skin barrier repair function according to claim 1 or 2, characterized in that the preparation method of component a is: strictly selecting crocus sativus pistils, treating the crocus sativus pistils by utilizing enzymatic hydrolysis, and filtering to obtain crocus sativus extracts;

4. A composition with skin barrier repair function according to claim 1 or 2, characterized in that the preparation method of component B is: washing wheat with second-stage reverse osmosis water, performing enzymolysis, extracting and separating to obtain oat beta-glucan, adding erythritol, stirring for dissolving, sterilizing, and freeze-drying to obtain component B for later use.

5. A composition with skin barrier repair function according to claim 1 or 2, characterized in that the preparation method of component E is: mannose, sodium hexametaphosphate and hexokinase react under a system of aqueous solution to generate mannose sodium phosphate, and after sterilizing and purifying the compound glycerol, the component E is obtained for standby.

6. A method for preparing the composition having a skin barrier repair function according to claim 1 or 2, comprising:

7. A method of preparing a composition having skin barrier repair function according to claim 6, wherein the composition is a milky viscous paste.

8. A method of preparing a composition having skin barrier repair function according to claim 6, wherein the temperature is raised to 80-85 ℃;

the heating and stirring time is 30-60min.

9. A formulation having skin barrier repair function, characterized by comprising the composition having skin barrier repair function according to claim 1 or 2.

10. Use of a formulation with skin barrier repair function, characterized in that it is applied as an additive in the preparation of a cosmetic product, water, milk, cream, essence, mask product for skin barrier repair, using the formulation according to claim 9;

the adding proportion is 0.1wt percent to 5wt percent.