CN116076449A - Artificial large-scale raising method for Buddha tortoise lecanii - Google Patents

Artificial large-scale raising method for Buddha tortoise lecanii Download PDF

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Publication number
CN116076449A
CN116076449A CN202310142154.3A CN202310142154A CN116076449A CN 116076449 A CN116076449 A CN 116076449A CN 202310142154 A CN202310142154 A CN 202310142154A CN 116076449 A CN116076449 A CN 116076449A
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tortoise
myrtle
fingered citron
lecanii
buddha
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胡进锋
虞赟
王俊
董文勇
陈�峰
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Fuzhou Customs Technical Center
Institute of Plant Protection of FAAS
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Fuzhou Customs Technical Center
Institute of Plant Protection of FAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Soil Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses an artificial large-scale raising method for Ericerus pela in Buddha, and relates to the field of insect raising. The invention comprises the following steps: 1) Cultivating myrtle in a greenhouse; 2) Throwing the bergamot to the bergamot; 3) Raising Buddha tortoise lecanii; 4) Dynamically monitoring the population; 5) The myrtle grown to adults is collected and stored. The method can be used for large-scale propagation of the Buddha tortoise lecanii, can realize uninterrupted propagation of the Buddha tortoise lecanii throughout the year, can meet the requirements of experimental research, and more importantly, can utilize the Buddha tortoise lecanii to artificially feed a large amount of parasitic or predatory natural enemy insects, provides sufficient insect sources for preventing and controlling the Buddha tortoise lecanii by utilizing the natural enemy insects, and can bring great economic benefit, social benefit and ecological benefit.

Description

Artificial large-scale raising method for Buddha tortoise lecanii
Technical Field
The invention relates to the technical field of insect breeding, in particular to an artificial large-scale breeding method for Buddha tortoise cerclage.
Background
The Buddha tortoise lecanii, ceroplastes floridensis Comstock, is an insect belonging to the genus lecania of the family lecaniaceae, hemiptera. Is extremely widely distributed in China, and has more than 100 plants at harm, wherein most plants belong to fruit trees and garden plants. Such as: tea, mandarin orange, guava, apple, persimmon, jujube, pear, peach, apricot, mango, loquat, cinnamon, etc. In the fruit tree mixed planting area, japanese tortoise lecanii can be transmitted mutually, and the method also brings great difficulty for thoroughly preventing and controlling. The breeding rate of the Buddha tortoise lecanii is high, the breeding quantity is large, and the Buddha tortoise lecanii starts to eat after 3 to 4 months. Meanwhile, the excrement can induce the occurrence of soot diseases, so that the plant is densely covered by black mould, photosynthesis is directly affected, and poor plant growth is caused.
Morphological characteristics of the fingered citron tortoise cercus: the first hatched nymphs, also called crawling nymphs, climb out of the gaps under the wax shells after hatching, are scattered on the leaves or twigs to be fixed, and the wander time generally only needs a few hours to more than one day. The wax secretion starts the next day after fixation, two rows of wax substances appear on the back of the body, and the periphery of the body also starts to secrete wax substances along with the appearance of the central wax substances in the next day. The clams showed starburst wax-like protrusion at the periphery about 3 and 4 days after fixation of the crawling nymphs, and the wax-coating of the first-instar nymphs was completed. Later, the wax layer gradually thickens with the molting of nymphs of each age. The nymphs of one age are more parasitic on the leaves, and the twigs are less, wherein the number of the nymphs is more, and the nymphs are often arranged along the vein sequence. After fixation, the nymphs do not climb, but the old nymphs migrate to the tender branches in a large quantity and are fixed and parasitic. The density of insect mouths on the branches greatly exceeds the number of the leaves in the adult stage, and the branches are densely distributed. The old wax shells of the spawned eggs remain stuck to the branches and leaves for a long time. Individuals in new and old generations are piled up on branches, and the plant growth is seriously affected by inducing coal diseases.
The chemical pesticide is a main method for preventing and controlling the fingered citron tortoise lecanii, but the outside of the fingered citron tortoise lecanii body is covered with a layer of thicker wax, so that the chemical pesticide has a protective effect on the fingered citron body, and the chemical pesticide is not easy to contact the fingered citron body through the wax layer, so that the ideal preventing and controlling effect is achieved. Compared with chemical pesticides, the natural enemy insect is used for preventing and controlling the fingered citron tortoise lecanii, and the method has the advantages of reducing pollution, protecting biological diversity, protecting environment and the like. The natural enemies of the Buddha tortoise and the Ericerus pela are mainly parasitic natural enemies, including Lepidoptera pela, lepidoptera hawaii, lepidoptera pela, lepidoptera yellow, lepidoptera longscurvy and Lepidoptera pela. In addition, the artificial large-scale propagation natural enemies such as the heterodera fragrans, the Aleurites rubra and the Aleurites mond are the precondition of the biological control of the Buddha tortoise cercus, can provide a large amount of Buddha tortoise cercus, are one of effective and key ways for realizing the continuous control of the Buddha tortcercus, and have no report about large-scale breeding of the Buddha tortcercus.
Therefore, providing a manual large-scale raising method for fingered citron tortoise lecanii is a problem to be solved by the person skilled in the art.
Disclosure of Invention
In view of this, the invention provides a method for artificial large-scale raising of Ericerus pela in Buddha.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
an artificial large-scale raising method for Buddha tortoise lecanii comprises the following steps:
1) Cultivating myrtle in a greenhouse;
2) Putting the Leptoradix Dactylicapni in the myrtle with the density of 400-600; watering the myrtle for 1 time every day to keep the soil moist;
3) Under the condition of raising the fingered citron in the insect-raising glass greenhouse, adopting a day-night temperature difference method, wherein the temperature in a photoperiod is 27+/-1 ℃, the temperature in a dark period is 25+/-1 ℃, the relative humidity is 70% -85%, the illumination is carried out for 12 hours, and the illumination intensity is 3000-5000 Lux;
4) Every 5-7 days, the density of the fingered citron tortoise meadow in the greenhouse is investigated, and population dynamic monitoring is carried out;
5) And (3) putting the fingered citron and the tortoise mealy for 80-90 days, and collecting and storing the myrtle full of adults when the number of adults is obviously increased.
Further, the cultivation method of the myrtle in the step 1) comprises the following steps:
(1) Cutting out 5-7 cm of lignified branches of myrtle in a cutting glass greenhouse, only keeping 2 leaves at the upper end, and inserting the lignified branches into a cutting culture disc filled with cutting media, wherein the specification of the cutting culture disc is 50cm multiplied by 48cm multiplied by 20cm;
(2) Shading the cuttage medium inserted into the branches in the step (1), earthing up and covering, and spraying water to thoroughly spray; conventional water management is carried out until the cutting branches of the myrtle root under the condition that soil is kept moist;
(3) Transplanting the rooted myrtle into a planting culture tray filled with a culture medium, wherein the specification of the planting culture tray is 50cm multiplied by 48cm multiplied by 30cm, transferring the planting culture tray into a myrtle culture glass greenhouse for culture, and performing conventional water management to keep soil moist, wherein flower fertilizers are applied once every 15 days;
(4) When the myrtle grows to 35-50 cm and the diameter of the stem reaches more than 3cm, transferring a planting culture tray for planting the myrtle to a worm-raising glass greenhouse for throwing the tortoise lecanii reptile in the Buddha.
Further, the lignified branch of the myrtle in the step (1) is soaked in a rooting agent solution with the weight percent of 0.25-0.40% for 12-16 hours and then is subjected to cuttage; the rooting agent is a growth regulator sold, and the main component is indoleacetic acid;
the cutting glass greenhouse conditions are that the temperature is 20-25 ℃, the relative humidity is 80-90%, the illumination is 8 hours, and the illumination intensity is 2500-3000 Lux;
the cutting medium comprises yellow sand, plant ash and perlite, wherein the yellow sand is prepared from the following raw materials: plant ash: the volume ratio of the perlite is 5-6:1-2:1-2;
the conditions of the glass greenhouse are cultivated, the temperature is 25-28 ℃, the relative humidity is 70-85%, the illumination time is 12 hours, and the illumination intensity is 3000-5000 Lux;
the culture medium comprises garden soil, leaf rot soil, rice chaff ash and coarse sand, wherein the garden soil is prepared from the following raw materials: leaf rot soil: rice chaff ash: the volume ratio of the coarse sand is 4-5:1-2:1-2.
Further, step (4) disinfects myrtle before releasing the tortoise lecanii, and fumigates the myrtle with bromomethane at 22 ℃ for 6 hours with 25 gm-3.
Furthermore, the cutting medium and the culture medium need to be disinfected before being used for planting the myrtle;
sterilizing by formalin sterilization, namely adding 200-300 ml of formalin (40% formaldehyde) into 25-30 kg of water, spraying into 1000 kg of culture medium, fully and uniformly stirring, stacking, covering a plastic film on the stack, and stacking for 48-72 hours; the plastic film is uncovered, after 1 to 2 weeks, the culture medium is loosened, and the medicine is used after the smell of the culture medium is diffused.
Further, in the method for throwing the fingered citron tortoise lecania in the step 2), after the fingered citron tortoise lecania becomes an adult, wrapping a paper strip on the myrtle growing on the adult, collecting the fingered citron tortoise lecania in a hatching full period by using the paper strip, connecting the paper strip on the fingered citron tortoise lecania in the fingered citron to the disinfected myrtle, and raising the fingered citron in a stocking glass greenhouse;
400-600 budworms are grafted on each myrtle, after the budworms are fixed on branches and leaves, the budworms are placed on a pest-culturing frame, watering and fertilizing are carried out on time until the egg-conception period of the female adult of the fingered citron tortoise lecanii is finished; the whole calendar period is 85 days.
Further, the method of storing in step 5) is as follows: collecting the myrtle grown on the adult tortoise lecanii, placing the myrtle at the temperature of 15+/-1 ℃ and the relative humidity of 70% -85%, and storing the myrtle under the condition of a climatic chamber with the illumination of 10L:14D, wherein the storage period is within 60-90 days.
Further, after the female insects begin to spawn in the step 5), the ventral surfaces of the scale bodies are gradually recessed towards the back, an egg cavity is formed below the wax shell, eggs are laid in the egg cavity, and female adults in the late stage of gestation of eggs are stored.
Compared with the prior art, the invention has the beneficial effects that:
the artificial feeding method of the Buddha tortoise lecanii is simple and easy to master, and particularly facilitates the relative consistency of the growth states of the Buddha tortoise lecanii, and the Buddha tortoise lecanii can be learned in a short period of time even if the feeder does not need related professional knowledge; the insect breeding rack is adopted for layered breeding, so that the occupied space is small, and the breeding cost is low;
the method can be used for large-scale propagation of the Buddha tortoise lecanii, can realize uninterrupted propagation of the Buddha tortoise lecanii throughout the year, can meet the requirements of experimental research, and more importantly, can utilize the Buddha tortoise lecanii to artificially feed a large amount of parasitic or predatory natural enemy insects, provides sufficient insect sources for preventing and controlling the Buddha tortoise lecanii by utilizing the natural enemy insects, and can bring great economic benefit, social benefit and ecological benefit.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Manually and massively raised fingered citron tortoise lecania:
(1) Infrastructure facility
Glass greenhouse: specification 15m x 8m (120 m) 2 ) The method comprises the steps of carrying out a first treatment on the surface of the The top of the greenhouse is provided with a sunlight plate and a retractable sunshade net, and is provided with 1.5 cooling and heating air conditioners, the glass greenhouse is divided into 4 independent rooms by using glass and a vacuum plate, one room is used as a medium treatment room, the other room is used as a myrtle cutting room (cutting glass greenhouse), and the other room is used as a myrtle planting room (cultivating glass greenhouse);
insect-raising glass greenhouse: the area is generally 25-40 m depending on the feeding scale 2 The window is provided with a stainless steel screen window, and is provided with an exhaust fan for ventilation and a 1.5 (25 m 2) or 3 (40 m 2) cold and warm air conditioner for controlling the indoor temperature of the insect breeding room; humidity is controlled by an Adam (YADU) SZK-J360 WiFi purification type intelligent humidifier, and insect raising frame switching time is controlled by a ZYT16G microcomputer time control switch;
storing the adult fingered citron tortoise lecanii in the late stage of gestation egg: the method is characterized in that the method adopts an MGC-800 HP-2 artificial climate box for preservation, and adopts the artificial climate box conditions of 15+/-1 ℃ and relative humidity of 70% -85% and illumination of 10 L:14D. The number of the artificial climate boxes is determined according to the number of the adult tortoise ceris in the later period of the gestation ovum.
Insect breeding rack: the insect breeding racks of 120cm multiplied by 50cm multiplied by 210cm are divided into 3 layers, a layer of toughened transparent glass with thicker texture is padded at the bottom of each layer, and 6 LED fluorescent lamps with 36W specifications are arranged at the top; the insect raising rack is provided with 80-mesh gauze at the periphery, the other side is provided with a sliding sand door, and each layer is divided into two areas by the 80-mesh gauze. Each layer of fluorescent lamps of each insect-raising frame is respectively provided with a manual switch;
(2) Suitable conditions for raising Buddha tortoise lecania
Temperature, humidity and illumination conditions in the insect-raising glass greenhouse: adopting a day and night temperature difference method, wherein the temperature in a photoperiod is 27+/-1 ℃, the temperature in a dark period is 25+/-1 ℃, the relative humidity is 70% -85%, the illumination time is 12 hours, and the illumination intensity is 3000-5000 Lux;
host conditions: high myrtle seedlings with the diameter of the stems reaching more than 3cm and 35-50 cm;
insect-breeding amount of single myrtle seedlings: about 500 crawlers are suitable;
(3) Preparation of Buddha tortoise lecanii source
The first breeding insect source can be collected from the Ericerus pela host plant such as Geranium, mandarin orange and fructus Mangifera Indicae, and then stored by using the method of the adult Testudinis in gestation period;
(4) Standard cultivation of host myrtle seedlings
Agent, fertilizer and plant regulator for cultivating myrtle seedling: seedling compound fertilizer (nitrogen: phosphorus: potassium element ratio is about 12:12:12); garden soil; leaf rot soil; rice chaff ash; yellow sand; plant ash; perlite; formalin; bromomethane; rooting powder; the materials are sold in the market, and brands and manufacturers can be selected according to local actual conditions;
the temperature, humidity and illumination conditions in the cutting glass greenhouse are that the temperature is 20-25 ℃, the relative humidity is 80-90%, the illumination time is 8 hours, and the illumination intensity is 2500-3000 Lux; the specifications of the cutting culture tray are 50cm multiplied by 48cm multiplied by 20cm;
cutting: cutting the lignified branches of the myrtle for 5-7 cm, only keeping 2 leaves at the upper end, soaking the base part for 1-2 hours by using rooting powder, and then inserting the cut leaves into a cutting medium; yellow sand as a cutting medium: plant ash: the volume ratio of the perlite is 5-6:1-2:1-2; before using the cutting medium, 200-300 ml of formalin (40% formaldehyde) is used for sterilizing the whole culture soil (25-30 kg of water is added into the formalin, the culture soil is sprayed into 1000 kg of culture soil, the culture soil is fully and uniformly mixed and then piled, plastic films and the like are covered on the piles, and the piles are piled for 48-72 hours so as to achieve the effect of thorough sterilization); watering thoroughly after cutting, covering with a mulching film, placing into a cutting room, shading by a sunshade net at noon, and watering at regular time (about 3-4 days) to keep moist. Rooting about 1 month after cutting.
Culturing the temperature, humidity and illumination conditions in a glass greenhouse, wherein the temperature is 25-28 ℃, the relative humidity is 70-85%, the illumination time is 12 hours, and the illumination intensity is 3000-5000 Lux; the specification of the planting culture plate is 50cm multiplied by 48cm multiplied by 30cm;
transplanting and culturing cutting seedlings: after rooting of the cuttage branches, transferring the rooting seedlings into a planting culture disc filled with a culture medium; the culture medium comprises garden soil, leaf rot soil, rice chaff ash and coarse sand, wherein the garden soil is: leaf rot soil: rice chaff ash: the volume ratio of the coarse sand is 4-5:1-2:1-2; the culture medium needs to be disinfected before use, and the method is the same as that of the cutting medium; watering every day, and applying the flower fertilizer every 15 days; when the myrtle grows to 35-50 cm, the myrtle is used for being inoculated with the tortoise lecanii reptile;
myrtle is sterilized at 22deg.C with 25gm of bromomethane -3 Fumigating for 6 hours, so as to ensure that no pest harm exists on the bromomethane.
(5) Breeding method of Buddha tortoise lecania
Adopting a day and night temperature difference method, wherein the temperature in a photoperiod is 27+/-1 ℃, the temperature in a dark period is 25+/-1 ℃, the relative humidity is 70% -85%, the illumination time is 12 hours, and the illumination intensity is 3000-5000 Lux; the method comprises the steps of carrying out a first treatment on the surface of the
Winding paper strips on the mature myrtle of the Tortoise in Buddha, collecting the reptiles by the paper strips in the hatching period, inoculating the paper strips full of the reptiles to the sterilized myrtle, and putting the myrtle in a 1) conditional glass greenhouse for feeding;
400-600 myrtus are grafted on each plant of myrtus, and after the reptiles are fixed on branches and leaves, the myrtus is placed on a pest-culturing frame, and watering and fertilizing are carried out on time. Until the egg-conception period of the female adult of the fingered citron tortoise lecanii is finished (85 days of the whole period);
after female insects begin to spawn, the ventral surface of the scale body gradually concave towards the back, an egg cavity is formed below the wax shell, and eggs are laid in the egg cavity and are orange;
(6) Annual production of fingered citron tortoise lecania
20m 2 Can be used for placing 12 insect-raising racks, and 4 white plastic basins with the thickness of 50cm multiplied by 35cm multiplied by 15cm can be placed on each layer of racks, namely, each insect-raising rack can accommodate 8 plastic basins. If the breeding is carried out according to the technical specifications, leaf beetles can be produced in each breeding room for each generation: 100 basins/insect-raising rack x 12 insect-raising racks x (400-600 heads)/plant=48-72 ten thousand heads;
the egg laying period of the adult fingered citron lecanii reaches 35d at 25 ℃, the fingered citron is parthenogenesis, the egg laying amount of the adult fingered citron lecanii is about 800, and each generation is about 80 days, and 4 generations can be realized each year. Then the number of leaf beetles which can be produced in a single breeding room per year is 192 to 288 tens of thousands, if 10 breeding rooms (namely 200m 2 The insect-raising space) and the annual yield can reach 1920-2880 ten thousand;
(7) Preservation of adult Tortoise in late stage of gestation egg
Seed protection mode: the myrtle of the Buddha tortoise cerclage adult in the late stage of the gestation egg is placed at the temperature of 15+/-1 ℃ and the relative humidity of 70-85 percent, and the storage period is within 60-90 days under the condition of illumination of 10L to 14D.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (8)

1. The artificial large-scale raising method for the fingered citron tortoise lecania is characterized by comprising the following steps of:
1) Cultivating myrtle in a greenhouse;
2) Putting the Leptoradix Dactylicapni in the myrtle with the density of 400-600; watering the myrtle for 1 time every day to keep the soil moist;
3) Under the condition of raising the fingered citron in the insect-raising glass greenhouse, adopting a day-night temperature difference method, wherein the temperature in a photoperiod is 27+/-1 ℃, the temperature in a dark period is 25+/-1 ℃, the relative humidity is 70% -85%, the illumination is carried out for 12 hours, and the illumination intensity is 3000-5000 Lux;
4) Every 5-7 days, the density of the fingered citron tortoise meadow in the greenhouse is investigated, and population dynamic monitoring is carried out;
5) And (3) putting the fingered citron and the tortoise mealy for 80-90 days, and collecting and storing the myrtle full of adults when the number of adults is obviously increased.
2. The artificial large-scale raising method of fingered citron tortoise lecanii according to claim 1, wherein the cultivation method of myrtle in step 1) comprises the following steps:
(1) Cutting out 5-7 cm of lignified branches of myrtle in a cutting glass greenhouse, only keeping 2 leaves at the upper end, and inserting the lignified branches into a cutting culture disc filled with cutting media, wherein the specification of the cutting culture disc is 50cm multiplied by 48cm multiplied by 20cm;
(2) Shading the cuttage medium inserted into the branches in the step (1), earthing up and covering, and spraying water to thoroughly spray; conventional water management is carried out until the cutting branches of the myrtle root under the condition that soil is kept moist;
(3) Transplanting the rooted myrtle into a planting culture tray filled with a culture medium, wherein the specification of the planting culture tray is 50cm multiplied by 48cm multiplied by 30cm, transferring the planting culture tray into a myrtle culture glass greenhouse for culture, and performing conventional water management to keep soil moist, wherein flower fertilizers are applied once every 15 days;
(4) When the myrtle grows to 35-50 cm and the diameter of the stem reaches more than 3cm, transferring a planting culture tray for planting the myrtle to a worm-raising glass greenhouse for throwing the tortoise lecanii reptile in the Buddha.
3. The artificial large-scale raising method of the fingered citron tortoise lecanii according to claim 2, wherein the step (1) is characterized in that the lignified branches of the myrtle are cut after being soaked in a rooting agent solution with the weight percent of 0.25-0.40% for 12-16 hours; the rooting agent is a growth regulator sold, and the main component is indoleacetic acid;
the cutting glass greenhouse conditions are that the temperature is 20-25 ℃, the relative humidity is 80-90%, the illumination is 8 hours, and the illumination intensity is 2500-3000 Lux;
the cutting medium comprises yellow sand, plant ash and perlite, wherein the yellow sand is prepared from the following raw materials: plant ash: the volume ratio of the perlite is 5-6:1-2:1-2;
the conditions of the glass greenhouse are cultivated, the temperature is 25-28 ℃, the relative humidity is 70-85%, the illumination time is 12 hours, and the illumination intensity is 3000-5000 Lux;
the culture medium comprises garden soil, leaf rot soil, rice chaff ash and coarse sand, wherein the garden soil is prepared from the following raw materials: leaf rot soil: rice chaff ash: the volume ratio of the coarse sand is 4-5:1-2:1-2.
4. The artificial large-scale raising method of Tortoise in Buddha according to claim 3, wherein step (4) comprises sterilizing myrtle before releasing Tortoise in Buddha, and using bromomethane 25gm at 22deg.C -3 Fumigating for 6 hours.
5. The method for artificial large-scale raising of cerclage in bergamot of claim 2, wherein said cutting medium and culture medium are sterilized before being used for planting myrtle;
sterilizing by formalin sterilization, namely adding 200-300 ml of formalin (40% formaldehyde) into 25-30 kg of water, spraying into 1000 kg of culture medium, fully and uniformly stirring, stacking, covering a plastic film on the stack, and stacking for 48-72 hours; the plastic film is uncovered, after 1 to 2 weeks, the culture medium is loosened, and the medicine is used after the smell of the culture medium is diffused.
6. The artificial large-scale raising method of the fingered citron tortoise lecania according to claim 1, wherein, step 2) the method of throwing the fingered citron tortoise lecania into the fingered citron, after the fingered citron tortoise lecania becomes an adult, winding a paper strip on the myrtle growing on the adult, collecting the fingered citron tortoise lecania in a hatching period of the fingered citron tortoise lecania by using the paper strip, connecting the paper strip of the fingered citron tortoise lecania to the sterilized myrtle, and raising the fingered citron in a glass greenhouse;
400-600 budworms are grafted on each myrtle, after the budworms are fixed on branches and leaves, the budworms are placed on a pest-culturing frame, watering and fertilizing are carried out on time until the egg-conception period of the female adult of the fingered citron tortoise lecanii is finished; the whole calendar period is 85 days.
7. The method for artificial mass rearing of fingered citron tortoise meadow of claim 1, wherein the method of storing in step 5) is: collecting the myrtle grown on the adult tortoise lecanii, placing the myrtle at the temperature of 15+/-1 ℃ and the relative humidity of 70% -85%, and storing the myrtle under the condition of a climatic chamber with the illumination of 10L:14D, wherein the storage period is within 60-90 days.
8. The artificial large-scale raising method of fingered citron tortoise lecania according to claim 1, wherein, after step 5) the female insect starts to spawn, the ventral surface of the lecania body gradually concave toward the back surface, the lower part of the wax shell presents an egg cavity in which the egg is laid, the egg is orange, and female adult in late stage of pregnant egg is stored.
CN202310142154.3A 2023-02-21 2023-02-21 Artificial large-scale raising method for Buddha tortoise lecanii Pending CN116076449A (en)

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