CN116036196B - Anti-hair loss hair-nourishing essence and preparation method thereof - Google Patents
Anti-hair loss hair-nourishing essence and preparation method thereof Download PDFInfo
- Publication number
- CN116036196B CN116036196B CN202211741902.1A CN202211741902A CN116036196B CN 116036196 B CN116036196 B CN 116036196B CN 202211741902 A CN202211741902 A CN 202211741902A CN 116036196 B CN116036196 B CN 116036196B
- Authority
- CN
- China
- Prior art keywords
- hair
- parts
- exosomes
- loss
- essence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003656 anti-hair-loss Effects 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 210000001808 exosome Anatomy 0.000 claims abstract description 67
- 201000004384 Alopecia Diseases 0.000 claims abstract description 53
- 230000003779 hair growth Effects 0.000 claims abstract description 46
- 230000000694 effects Effects 0.000 claims abstract description 36
- 230000001737 promoting effect Effects 0.000 claims abstract description 31
- 238000000855 fermentation Methods 0.000 claims abstract description 30
- 230000004151 fermentation Effects 0.000 claims abstract description 30
- 241000196324 Embryophyta Species 0.000 claims abstract description 26
- 241000186660 Lactobacillus Species 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 15
- 239000000706 filtrate Substances 0.000 claims abstract description 15
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 15
- 239000006166 lysate Substances 0.000 claims abstract description 15
- 241001523681 Dendrobium Species 0.000 claims abstract description 14
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 14
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 14
- 240000007651 Rubus glaucus Species 0.000 claims abstract description 14
- 235000011034 Rubus glaucus Nutrition 0.000 claims abstract description 14
- 235000009122 Rubus idaeus Nutrition 0.000 claims abstract description 14
- 235000008434 ginseng Nutrition 0.000 claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 13
- 239000000047 product Substances 0.000 claims abstract description 13
- 239000000203 mixture Substances 0.000 claims description 44
- 230000003676 hair loss Effects 0.000 claims description 20
- 208000024963 hair loss Diseases 0.000 claims description 20
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 14
- 239000003963 antioxidant agent Substances 0.000 claims description 14
- 230000003078 antioxidant effect Effects 0.000 claims description 14
- 239000003906 humectant Substances 0.000 claims description 14
- 239000002904 solvent Substances 0.000 claims description 14
- 241000208340 Araliaceae Species 0.000 claims description 13
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical group CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000000043 antiallergic agent Substances 0.000 claims description 11
- 239000003637 basic solution Substances 0.000 claims description 9
- 230000003750 conditioning effect Effects 0.000 claims description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 8
- 229940015975 1,2-hexanediol Drugs 0.000 claims description 7
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims description 7
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 7
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 claims description 6
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 claims description 6
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims description 6
- 241000235342 Saccharomycetes Species 0.000 claims description 6
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims description 6
- 235000010385 ascorbyl palmitate Nutrition 0.000 claims description 6
- VKIJBOMCWLTQBD-UHFFFAOYSA-N benzoic acid N-(2-hydroxyphenyl)propanamide Chemical group C(C1=CC=CC=C1)(=O)O.C(CC)(=O)NC1=C(C=CC=C1)O VKIJBOMCWLTQBD-UHFFFAOYSA-N 0.000 claims description 6
- 239000004359 castor oil Substances 0.000 claims description 6
- 235000019438 castor oil Nutrition 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 229950006238 nadide Drugs 0.000 claims description 6
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 229920002385 Sodium hyaluronate Polymers 0.000 claims description 3
- 229960005150 glycerol Drugs 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 229940010747 sodium hyaluronate Drugs 0.000 claims description 3
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims 1
- 231100000360 alopecia Toxicity 0.000 abstract description 27
- 108010066551 Cholestenone 5 alpha-Reductase Proteins 0.000 abstract description 18
- 210000004209 hair Anatomy 0.000 abstract description 12
- 230000002829 reductive effect Effects 0.000 abstract description 7
- 230000002401 inhibitory effect Effects 0.000 abstract description 6
- 244000131316 Panax pseudoginseng Species 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 210000003780 hair follicle Anatomy 0.000 description 28
- 238000012360 testing method Methods 0.000 description 14
- 210000003491 skin Anatomy 0.000 description 13
- 241000699670 Mus sp. Species 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 11
- 238000010998 test method Methods 0.000 description 10
- 230000035755 proliferation Effects 0.000 description 9
- 230000003658 preventing hair loss Effects 0.000 description 6
- 210000004761 scalp Anatomy 0.000 description 6
- 108090000854 Oxidoreductases Proteins 0.000 description 4
- 102000004316 Oxidoreductases Human genes 0.000 description 4
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000000442 hair follicle cell Anatomy 0.000 description 4
- 230000003660 hair regeneration Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 3
- 230000036770 blood supply Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 201000002996 androgenic alopecia Diseases 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000009583 hair follicle growth Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000004089 microcirculation Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 229960003604 testosterone Drugs 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 1
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 238000011740 C57BL/6 mouse Methods 0.000 description 1
- 240000007371 Cuscuta campestris Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010020112 Hirsutism Diseases 0.000 description 1
- 241000830535 Ligustrum lucidum Species 0.000 description 1
- ZFMITUMMTDLWHR-UHFFFAOYSA-N Minoxidil Chemical compound NC1=[N+]([O-])C(N)=CC(N2CCCCC2)=N1 ZFMITUMMTDLWHR-UHFFFAOYSA-N 0.000 description 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 240000001341 Reynoutria japonica Species 0.000 description 1
- 235000018167 Reynoutria japonica Nutrition 0.000 description 1
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 208000030695 Sparse hair Diseases 0.000 description 1
- 208000001871 Tachycardia Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000001467 acupuncture Methods 0.000 description 1
- 208000004631 alopecia areata Diseases 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 229940107666 astragalus root Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 229960004199 dutasteride Drugs 0.000 description 1
- JWJOTENAMICLJG-QWBYCMEYSA-N dutasteride Chemical compound O=C([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)N[C@@H]4CC3)C)CC[C@@]21C)NC1=CC(C(F)(F)F)=CC=C1C(F)(F)F JWJOTENAMICLJG-QWBYCMEYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 229960004039 finasteride Drugs 0.000 description 1
- DBEPLOCGEIEOCV-WSBQPABSSA-N finasteride Chemical compound N([C@@H]1CC2)C(=O)C=C[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H](C(=O)NC(C)(C)C)[C@@]2(C)CC1 DBEPLOCGEIEOCV-WSBQPABSSA-N 0.000 description 1
- 230000003450 growing effect Effects 0.000 description 1
- 230000003752 improving hair Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229960003632 minoxidil Drugs 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001107 psychogenic effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000006794 tachycardia Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 229940126673 western medicines Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Birds (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of hair care agents, and particularly relates to an anti-hair-loss hair-growing essence and a preparation method thereof. The anti-hair loss and hair-growth essence contains active ingredients such as lactobacillus fermentation lysate, two-split yeast fermentation product filtrate, plant exosomes and the like, wherein the plant exosomes are at least one of ginseng exosomes, raspberry exosomes and dendrobium exosomes; on the other hand, the alopecia is effectively reduced by effectively inhibiting the activity of 5 alpha reductase, and the effects of preventing alopecia and promoting hair growth are comprehensively achieved.
Description
Technical Field
The invention belongs to the technical field of hair care agents, and particularly relates to an anti-hair-loss hair-growing essence and a preparation method thereof.
Background
In recent years, the proportion of the population suffering from alopecia is higher and the population suffering from alopecia is in a trend of younger age, and research and study show that alopecia phenomena comprise alopecia areata, psychogenic alopecia and androgenic alopecia (seborrheic alopecia), wherein more than 90% of the alopecia population is androgenic alopecia, and the mechanism is mainly that testosterone is converted into Dihydrotestosterone (DHT) with higher biological activity under the action of 5 alpha-reductase, so that the binding of testosterone to a receptor is competitively inhibited, the metabolic process is inhibited, static hair follicles are prevented from entering a growing period, hair follicles are gradually contracted until the hair follicles disappear, and alopecia is caused.
In the alopecia treatment, the western medicines commonly used in clinic are minoxidil and finasteride, but the two medicines are easy to relapse after stopping taking, and can also cause side effects such as skin irritation, scalp itching, facial and hand hirsutism, dizziness, anorexia, tachycardia and the like. In addition, although hair transplantation has a certain therapeutic effect, the clinical application of the hair transplantation is limited due to the large surgical trauma and high cost and the need of multiple surgeries. The effect of non-drug treatment such as acupuncture is also not ideal.
At present, research reports on alopecia treatment by using plant extracts such as traditional Chinese medicines are more and more, and plant source components are relatively safe and effective and are convenient to use. For example, the single or mixed use of astragalus root, fleece-flower root, glossy privet fruit, dodder seed and the like has the effects of stimulating the growth of hair follicles of mice cultivated in vitro, increasing the number of hair follicles, accelerating the hair regeneration speed and the like, however, the simple treatment of the traditional Chinese medicine extract has long treatment period and slow effect, and often causes physiological alopecia when the hair regeneration is carried out, and the effect of comprehensively improving the alopecia is also poor when the speed of promoting the hair regeneration of the traditional Chinese medicine extract is lower than that of the physiological alopecia.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide the hair loss preventing and hair growing essence and the preparation method thereof, which can effectively inhibit the activity of 5 alpha reductase to reduce physiological alopecia, and can promote the proliferation of hair follicle cells to promote regeneration of new hair to comprehensively achieve the hair loss preventing and hair growing effects.
Based on the above purpose, the technical scheme adopted by the invention is as follows:
In a first aspect, the present invention provides an anti-hair-growing composition comprising the following components: lactobacillus fermentation lysate, saccharomyces cerevisiae fermentation product filtrate and plant exosomes;
The plant exosome is at least one of ginseng exosome, raspberry exosome and dendrobium exosome.
The research shows that the hair growth and regeneration depend on the blood supply of the organism to hair follicles, the hair follicles are surrounded by rich vascular beds, when microcirculation is blocked, the blood flow rate in the microcirculation is reduced, the normal supply of nutrients required by the organism to the hair follicles is affected, the hair follicles lack of nutrients, and alopecia occurs, and the hair loss prevention hair care composition can effectively promote the blood circulation of scalp, increase the blood supply of hair papilla, improve the nutrition of hair follicles, slow down the miniaturization process of hair follicles, increase the number of hair follicles and promote hair growth. In addition, the hair loss prevention and hair growth promoting composition can effectively inhibit the activity of 5 alpha reductase, effectively reduce physiological alopecia and comprehensively achieve the effect of improving hair loss prevention and hair growth promoting.
Preferably, the plant exosomes consist of ginseng exosomes, raspberry exosomes and dendrobium exosomes in a weight ratio of 1 (0.2-0.6): 1-1.5.
Experiments show that when the ginseng exosomes, the raspberry exosomes and the dendrobium exosomes are compounded according to the proportion, the prepared hair-loss preventing and hair-growing composition shows better hair-loss preventing and hair-growing effects.
Preferably, the weight ratio of the ginseng exosomes, the raspberry exosomes and the dendrobium exosomes is 1 (0.4-0.5): 1.2-1.5.
Experiments show that when the ginseng exosomes, the raspberry exosomes and the dendrobium exosomes are compounded according to the proportion, the prepared hair-loss preventing and hair-growing composition has better hair-loss preventing and hair-growing effects.
Preferably, the weight ratio of lactobacillus fermentation lysate, the filtrate of the fermentation product of the two yeasts to the plant exosome is (10-17): 8-12): 1.
Experiments show that when lactobacillus fermentation lysate, yeast fermentation product filtrate and plant exosome are compounded according to the proportion, the prepared hair loss preventing hair growth promoting composition shows better hair loss preventing hair growth promoting effect.
Preferably, the weight ratio of lactobacillus fermentation lysate, the filtrate of the fermentation product of the two yeasts to the plant exosomes is (10-15): 10:1.
Experiments show that when lactobacillus fermentation lysate, yeast fermentation product filtrate and plant exosome are compounded according to the proportion, the prepared hair loss preventing hair growth promoting composition shows better hair loss preventing hair growth promoting effect.
In a second aspect, the invention provides an anti-hair loss and hair growth promoting essence, which contains the anti-hair loss and hair growth promoting composition.
Preferably, the anti-hair loss and hair-growing essence comprises the following components in parts by weight: 20 to 45 parts of hair-growing composition with anti-alopecia effect, 6 to 13 parts of humectant, 1 to 5 parts of skin conditioning agent, 3 to 7 parts of antioxidant, 0.1 to 0.5 part of anti-allergic agent, 1 to 3 parts of solubilizer and 80 to 120 parts of water.
Preferably, the anti-hair loss and hair-growing essence comprises the following components in parts by weight: 30-40 parts of hair-growing composition, 6-13 parts of humectant, 1-5 parts of skin conditioner, 3-7 parts of antioxidant, 0.1-0.5 part of anti-allergic agent, 1-3 parts of solubilizer and 80-120 parts of water.
Preferably, the humectant is at least one of 1, 2-hexanediol, glycerol, and sodium hyaluronate; the skin conditioner is at least one of nicotinamide, saccharomycete polypeptide and nicotinamide adenine dinucleotide; the antioxidant is p-hydroxyacetophenone and/or ascorbyl palmitate; the anti-allergic agent is hydroxyphenyl propionamide benzoic acid; the solubilizer is PEG-40 hydrogenated castor oil.
In a third aspect, the present invention provides a method for preparing the above anti-hair loss and hair growth promoting essence, comprising the steps of:
(1) Mixing humectant, solubilizer and water, heating to 90-100 ℃, stirring uniformly to obtain a basic solution, and cooling the basic solution to 60-65 ℃ for standby;
(2) Adding an antioxidant into the basic solution, homogenizing for 1-2 min, then adding an anti-allergic agent and a skin conditioning agent, homogenizing for 1-2 min, and then adding an anti-hair loss composition, homogenizing for 1-2 min to obtain a mixed solution; controlling the homogenizing temperature to be 60-65 ℃, preserving the temperature of the mixed solution at 60-65 ℃ for 0.5-1 h after the homogenizing is finished, and cooling to room temperature to prepare the anti-hair loss and hair-growing essence.
Compared with the prior art, the invention has the following beneficial effects:
The invention provides an anti-hair-loss hair-growing composition and an essence containing the same, which are characterized in that on one hand, the anti-hair-loss hair-growing essence promotes scalp blood circulation, increases hair papilla blood supply, improves hair follicle nutrition, slows down hair follicle miniaturization process, increases hair follicle number and promotes hair growth; on the other hand, the alopecia is effectively reduced by effectively inhibiting the activity of 5 alpha reductase, and the effects of preventing alopecia and promoting hair growth are comprehensively achieved.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific examples. It will be appreciated by persons skilled in the art that the specific embodiments described herein are for purposes of illustration only and are not intended to be limiting.
The test methods used in the examples are conventional methods unless otherwise specified; the materials, reagents and the like used, unless otherwise specified, are all commercially available.
Example 1
The embodiment provides an anti-hair loss and hair-growing essence, which comprises the following components in parts by weight: 20 to 45 parts of hair-growing composition with anti-alopecia effect, 6 to 13 parts of humectant, 1 to 5 parts of skin conditioning agent, 3 to 7 parts of antioxidant, 0.1 to 0.5 part of anti-allergic agent, 1 to 3 parts of solubilizer and 80 to 120 parts of water.
Wherein the anti-hair loss hair-growing composition is formed by compounding lactobacillus fermentation lysate, two-split yeast fermentation product filtrate and plant exosome according to the weight ratio of (10-17) (8-12): 1; the plant exosome is compounded with ginseng exosome, raspberry exosome and dendrobium exosome in the weight ratio of 1 (0.2-0.6) to 1-1.5.
The humectant is at least one of 1, 2-hexanediol, glycerol and sodium hyaluronate, preferably 1, 2-hexanediol; the skin conditioner is at least one of nicotinamide, saccharomycete polypeptide and nicotinamide adenine dinucleotide; the antioxidant is p-hydroxyacetophenone and/or ascorbyl palmitate; the anti-allergic agent is hydroxyphenyl propionamide benzoic acid; the solubilizer is PEG-40 hydrogenated castor oil.
The preparation method of the hair loss prevention and hair growth promoting essence comprises the following steps:
(1) Mixing humectant, solubilizer and water, heating to 90-100 ℃, stirring uniformly to obtain a basic solution, and cooling the basic solution to 60-65 ℃ for standby;
(2) Adding an antioxidant into the basic solution, homogenizing for 1-2 min, then adding an anti-allergic agent and a skin conditioning agent, homogenizing for 1-2 min, and then adding an anti-hair loss composition, homogenizing for 1-2 min to obtain a mixed solution; controlling the homogenizing temperature to be 60-65 ℃, preserving the temperature of the mixed solution at 60-65 ℃ for 0.5-1 h after the homogenizing is finished, and cooling to room temperature to prepare the anti-hair loss and hair-growing essence.
In order to explore the proper adding amount of the anti-hair-loss and hair-growth composition in the anti-hair-loss and hair-growth essence, only the weight part of the anti-hair-loss and hair-growth composition is taken as a single variable, the weight parts of other components are kept unchanged, the proportion of each component in the anti-hair-loss and hair-growth composition is kept unchanged, and the anti-hair-loss and hair-growth essence used in a specific test comprises the following components in parts by weight:
10-50 parts of anti-hair loss hair-growing composition; 10 parts of humectant 1, 2-hexanediol; the skin conditioning agent comprises 2 parts of nicotinamide, 2 parts of saccharomycete polypeptide and 1 part of nicotinamide adenine dinucleotide, and the total weight is 5 parts; the antioxidant comprises 5 parts of p-hydroxyacetophenone and 1 part of ascorbyl palmitate, and the total amount is 6 parts; 0.3 parts of an anti-sensitizer, namely, hydroxyphenyl propionamide benzoic acid; 2 parts of PEG-40 hydrogenated castor oil serving as a solubilizer and 100 parts of water.
Wherein the weight ratio of lactobacillus fermentation lysate, yeast fermentation product filtrate and plant exosome in the hair-growth preventing composition is 15:10:1, and the weight ratio of ginseng exosome, raspberry exosome and dendrobium exosome in the plant exosome is 1:0.5:1.5.
The weight parts of the hair-loss preventing composition in the hair-loss preventing composition are shown in table 1, and the 5a reductase inhibition rate and the number of hair follicles on the back of the mice of each hair-loss preventing composition are counted according to the following effect test method, and the results are shown in table 1.
(1) 5 Alpha reductase inhibition effect test
300. Mu.L of PBS buffer (pH 7.4), 500. Mu.L of 5. Alpha. Reductase of 0.5mg/mL, 50. Mu.L of a sample to be tested, 50. Mu.L of 50% ethanol and 100. Mu.L of NADPH are sequentially added to a centrifuge tube, rapidly mixed uniformly for 3s in a vortex mixer, reacted in a constant temperature water bath at 37℃for 30min, rapidly sampled for 400. Mu.L at the beginning of the reaction (at time t 0) and for 30min (at time t 30) respectively, rapidly added with 800. Mu.L of methanol for inactivating the enzyme, and vortexed for 5s to terminate the reaction. And detecting the concentration of 5 alpha reductase at time t 0 and time t 30 of the sample to be detected respectively, and calculating the concentration difference value of the sample to be detected. Detecting the dutasteride serving as a standard substance to obtain a concentration difference value which is a standard concentration difference value; the concentration difference obtained by replacing the test sample with 50 μl of PBS buffer is the blank concentration difference. The inhibition rate of the test sample to the 5 alpha reductase activity is calculated as follows:
Inhibition ratio (%) of 5α reductase activity= (standard concentration difference-test group concentration difference)/(standard concentration difference-blank group concentration difference).
(2) Test for promoting proliferation of mouse hair follicle cells
C57BL/6 pure-bred mice with the weight of 15-20g are taken, the male and female mice are used together, the back hair of the mice is pulled out by using a mixture of rosin and paraffin under the mild anesthesia of diethyl ether, the area is about 3cm multiplied by 4cm, and the mice are grouped, namely a normal control group and each test group. The next day after dehairing, each sample is uniformly smeared on the skin of the dehairing area at the back of each group of mice, the dosage of each test group is 10ml/kg, the same volume of physiological saline group is given to the control group, 1 time/day is continued for 10 days, the skin of the dehairing area at the back of the mice is taken after the administration for 10 days, formalin is used for fixing, pathological sections are made, the hair follicle growth condition is observed, the hair follicle number of 10 mice is counted by each sample, the average value is shown in table 1, and the average value of the hair follicle number of the mice in the control group is 102 hair follicle number/visual field.
(3) Volunteers are recruited to carry out hair loss prevention and hair growth effect test
50 Patients with alopecia are recruited, wherein men account for 80%, women account for 20%, the disease course is 10 years at the longest, 2 years at the shortest, and the ages are between 20 and 60 years. The essence samples of this example were used as test agents, and the patients were applied to the affected parts with the above test agents by applying 2ml each time, 2 times a day (once a day in the morning and evening), and 4 weeks of administration were a single treatment course, followed by 4 continuous treatment courses with no interruption in the middle.
Measurement standard:
The effect is shown: the hair loss phenomenon is avoided, the hair is thick and blackened gradually at the position where the scalp is bald and sparse;
the method is effective: no alopecia phenomenon, and sparse hair grows at the scalp baldness and sparseness;
invalidation: has alopecia phenomenon, and no hair growth occurs at the bald part of the scalp.
The total effectiveness (including efficacy and effectiveness) of the clinical treatment on 50 patients is counted as shown in table 1.
TABLE 1
The test results are shown in table 1, along with the increase of the addition amount of the anti-hair-loss composition in the anti-hair-loss essence, the inhibition rate of the anti-hair-loss essence to the 5 alpha reductase tends to be increased and then decreased, it is shown that the inhibition effect on 5α reductase is poor when the content of the hair-growth preventing composition is less than 20 parts by weight, and that the inhibition rate of 5α reductase is less changed when the content of the hair-growth preventing composition is more than 45 parts by weight.
With the increase of the addition amount of the anti-hair-loss and hair-growth combination in the anti-hair-growth essence, the number of the hair follicles of the mice shows a trend of increasing firstly and then stabilizing and then decreasing, and it is presumed that when the addition amount of the anti-hair-loss and hair-growth combination in the anti-hair-loss and hair-growth essence is between 20 parts and 45 parts, especially between 30 and 40 parts, the effect of promoting the hair follicle growth is better and more stable, and then the decrease of the number of the hair follicles is presumed to be due to the fact that the skin is difficult to absorb excessive essence due to the too high concentration of the essence, but is unfavorable for the proliferation of the hair follicles.
When the adding amount of the anti-hair-loss and hair-growth composition in the anti-hair-growth essence is 20-45 parts, the total effective rate of the anti-hair-loss and hair-growth composition for clinical treatment of patients suffering from alopecia reaches 100%.
Example 2
The purpose of this example is to analyze the effect of specific components of the hair-setting composition on hair-setting effect, and the test method is as follows:
In the embodiment, the weight ratio of lactobacillus fermentation lysate, yeast two fermentation product filtrate and plant exosome in the hair loss prevention hair growth combination is taken as a single factor variable, and the hair loss prevention hair growth essence comprises the following components in parts by weight:
40 parts of an anti-hair loss hair-growing composition; 10 parts of humectant 1, 2-hexanediol; the skin conditioning agent comprises 2 parts of nicotinamide, 2 parts of saccharomycete polypeptide and 1 part of nicotinamide adenine dinucleotide, and the total weight is 5 parts; the antioxidant comprises 5 parts of p-hydroxyacetophenone and 1 part of ascorbyl palmitate, and the total amount is 6 parts; 0.3 parts of an anti-sensitizer, namely, hydroxyphenyl propionamide benzoic acid; 2 parts of PEG-40 hydrogenated castor oil serving as a solubilizer and 100 parts of water. Wherein the weight ratio of the ginseng exosomes to the raspberry exosomes to the dendrobium exosomes in the plant exosomes is 1:0.5:1.5.
Wherein the weight ratio of lactobacillus fermentation lysate (A), yeast fermentation product filtrate (B) and plant exosome (C) in the hair loss preventing hair growth promoting composition is shown in Table 2.
Referring to the test method for the inhibition effect of 5α reductase on each sample and the test method for promoting proliferation of mouse hair follicle cells described in example 1, the inhibition rate of 5α reductase and the mean number of hair follicles of each sample described in this example were counted, as shown in table 2.
Meanwhile, referring to the clinical test method described in example 1, the test of the essence sample described in table 2 was carried out on the patients suffering from alopecia, and the total effective rate on the patients suffering from alopecia was counted, and the results are shown in table 2.
TABLE 2
As is clear from the results shown in Table 2, when the ratio of the lactobacillus fermentation lysate, the yeast fermentation filtrate, and the plant exosome in the hair-growing composition is within the specific ratio range (10-17): 8-12): 1, the hair follicle proliferation promoting and 5. Alpha. Reductase activity inhibiting effects are more excellent, and when the ratio exceeds the above ratio range, the hair follicle proliferation promoting and 5. Alpha. Reductase activity inhibiting effects are reduced, and the total effective rate is also reduced.
Example 3
The present example aims at analyzing the influence of the composition of plant exosomes in the hair-growth composition on the hair-growth effect of hair-growth, and the test method is as follows:
in the embodiment, the weight ratio of ginseng exosomes, raspberry exosomes and dendrobium exosomes in plant exosomes is used as a single factor variable, and the anti-hair loss hair-growing essence comprises the following components in parts by weight:
40 parts of an anti-hair loss hair-growing composition; 10 parts of humectant 1, 2-hexanediol; the skin conditioning agent comprises 2 parts of nicotinamide, 2 parts of saccharomycete polypeptide and 1 part of nicotinamide adenine dinucleotide, and the total weight is 5 parts; the antioxidant comprises 5 parts of p-hydroxyacetophenone and 1 part of ascorbyl palmitate, and the total amount is 6 parts; 0.3 parts of an anti-sensitizer, namely, hydroxyphenyl propionamide benzoic acid; 2 parts of PEG-40 hydrogenated castor oil serving as a solubilizer and 100 parts of water. Wherein the weight ratio of lactobacillus fermentation lysate, yeast two fermentation product filtrate and plant exosome in the hair loss preventing hair growth promoting composition is 15:10:1. The weight ratios of the ginseng exosomes (D), the raspberry exosomes (E) and the dendrobium exosomes (F) in the plant exosomes are shown in table 3.
Referring to the test method for the inhibition effect of 5α reductase on each sample and the test method for promoting proliferation of mouse hair follicle cells described in example 1, the inhibition rate of 5α reductase and the mean number of hair follicles of each sample described in this example were counted, as shown in table 3.
Meanwhile, referring to the clinical test method described in example 1, the test of the essence sample described in table 3 was carried out on the patients suffering from alopecia, and the total effective rate on the patients suffering from alopecia was counted, and the results are shown in table 3.
TABLE 3 Table 3
As shown in Table 3, when the ratio of the ginseng exosome, the raspberry exosome and the dendrobium exosome is 1 (0.2-0.6): 1-1.5, the effect of promoting hair follicle proliferation and inhibiting 5 alpha reductase activity is more excellent, and when the ratio is beyond the above ratio, the effect of promoting hair follicle proliferation or inhibiting 5 alpha reductase activity is reduced, and the total effective rate is also reduced.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.
Claims (8)
1. An anti-hair loss and hair-growing composition, which is characterized by comprising the following components: lactobacillus fermentation lysate, saccharomyces cerevisiae fermentation product filtrate and plant exosomes;
The plant exosomes consist of ginseng exosomes, raspberry exosomes and dendrobium exosomes in a weight ratio of 1 (0.2-0.6): 1-1.5;
The weight ratio of the lactobacillus fermentation lysate to the filtrate of the two-split yeast fermentation product to the plant exosome is (10-17): 8-12): 1.
2. The hair-loss preventing and hair-growing composition according to claim 1, wherein the weight ratio of ginseng exosomes, raspberry exosomes and dendrobium exosomes is 1 (0.4-0.5): 1.2-1.5.
3. The hair-loss preventing composition according to claim 1, wherein the weight ratio of lactobacillus fermentation lysate, yeast fermentation filtrate to plant exosomes is (10-15): 10:1.
4. An anti-hair loss and hair growth promoting essence characterized in that the anti-hair loss and hair growth promoting essence contains the anti-hair loss and hair growth promoting composition according to any one of claims 1 to 3.
5. The anti-hair loss and hair growth promoting essence according to claim 4, wherein the anti-hair loss and hair growth promoting essence comprises the following components in parts by weight: 20 to 45 parts of hair-growing composition with anti-alopecia effect, 6 to 13 parts of humectant, 1 to 5 parts of skin conditioning agent, 3 to 7 parts of antioxidant, 0.1 to 0.5 part of anti-allergic agent, 1 to 3 parts of solubilizer and 80 to 120 parts of water.
6. The anti-hair loss and hair growth promoting essence according to claim 5, wherein the anti-hair loss and hair growth promoting essence comprises the following components in parts by weight: 30-40 parts of hair-growing composition, 6-13 parts of humectant, 1-5 parts of skin conditioner, 3-7 parts of antioxidant, 0.1-0.5 part of anti-allergic agent, 1-3 parts of solubilizer and 80-120 parts of water.
7. The anti-hair loss and hair growth promoting essence according to claim 5 or 6, wherein the humectant is at least one of 1, 2-hexanediol, glycerin, and sodium hyaluronate; the skin conditioner is at least one of nicotinamide, saccharomycete polypeptide and nicotinamide adenine dinucleotide; the antioxidant is p-hydroxyacetophenone and/or ascorbyl palmitate; the anti-allergic agent is hydroxyphenyl propionamide benzoic acid; the solubilizer is PEG-40 hydrogenated castor oil.
8. A method for preparing the hair loss preventing and growing essence according to any one of claims 4 to 7, which is characterized by comprising the following steps:
(1) Mixing humectant, solubilizer and water, heating to 90-100 ℃, stirring uniformly to obtain a basic solution, and cooling the basic solution to 60-65 ℃ for standby;
(2) Adding an antioxidant into the basic solution, homogenizing for 1-2 min, then adding an anti-allergic agent and a skin conditioning agent, homogenizing for 1-2 min, and then adding an anti-hair loss composition, homogenizing for 1-2 min to obtain a mixed solution; controlling the homogenizing temperature to be 60-65 ℃, preserving the temperature of the mixed solution at 60-65 ℃ for 0.5-1 h after the homogenizing is finished, and cooling to room temperature to prepare the anti-hair loss and hair-growing essence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211741902.1A CN116036196B (en) | 2022-12-29 | 2022-12-29 | Anti-hair loss hair-nourishing essence and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211741902.1A CN116036196B (en) | 2022-12-29 | 2022-12-29 | Anti-hair loss hair-nourishing essence and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116036196A CN116036196A (en) | 2023-05-02 |
| CN116036196B true CN116036196B (en) | 2024-06-04 |
Family
ID=86115924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211741902.1A Active CN116036196B (en) | 2022-12-29 | 2022-12-29 | Anti-hair loss hair-nourishing essence and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116036196B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117379465B (en) * | 2023-11-07 | 2024-06-04 | 江苏亨瑞生物医药科技有限公司 | Hair-growing and nourishing composition and preparation method and application thereof |
| CN117338687A (en) * | 2023-11-17 | 2024-01-05 | 开远市洁白洗涤用品有限公司 | Hair-protecting and developing composition and preparation method and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108289921A (en) * | 2015-09-30 | 2018-07-17 | 株式会社爱茉莉太平洋 | For pre-Anti-hair loss or composition trichogenous, containing excretion body sample vesica derived from ginseng |
| CN110585076A (en) * | 2019-09-26 | 2019-12-20 | 广州艾蓓生物科技有限公司 | Prebiotics and probiotic composition, and preparation method and application thereof |
| CN112175064A (en) * | 2020-10-12 | 2021-01-05 | 珠海乐维再生医学科技有限公司 | Preparation method of KGF protein exosome, KGF protein exosome dry powder, application and hair care agent |
| CN113304065A (en) * | 2021-05-08 | 2021-08-27 | 广州宝思利生物科技有限公司 | Scalp care composition and application thereof |
| CN113846046A (en) * | 2021-10-22 | 2021-12-28 | 科丝美诗(中国)化妆品有限公司 | Dendrobium-derived exosome-like vesicle, preparation method thereof and application thereof in alopecia improvement |
| CN114191365A (en) * | 2021-12-31 | 2022-03-18 | 盈馨堂(武汉)商贸有限公司 | Anti-hair-loss and hair-growing essence and preparation method thereof |
| CN114306432A (en) * | 2021-12-21 | 2022-04-12 | 广州远想生物科技股份有限公司 | Plant exosome composition with anti-alopecia and hair-growing functions and preparation method thereof |
| CN114401729A (en) * | 2019-09-02 | 2022-04-26 | 耶迪特普大学 | Use of plant exosomes for enhancing skin cell viability and hair growth capacity |
| CN114392303A (en) * | 2022-02-17 | 2022-04-26 | 浙江卫未生物医药科技有限公司 | Exosome mixture for treating alopecia areata |
| CN115054566A (en) * | 2022-06-29 | 2022-09-16 | 四川语安生物科技有限公司 | Scalp aging resisting composition and application thereof |
| CN115300404A (en) * | 2022-04-02 | 2022-11-08 | 苏州绿叶日用品有限公司 | Essence for hair and preparation method thereof |
-
2022
- 2022-12-29 CN CN202211741902.1A patent/CN116036196B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108289921A (en) * | 2015-09-30 | 2018-07-17 | 株式会社爱茉莉太平洋 | For pre-Anti-hair loss or composition trichogenous, containing excretion body sample vesica derived from ginseng |
| CN114401729A (en) * | 2019-09-02 | 2022-04-26 | 耶迪特普大学 | Use of plant exosomes for enhancing skin cell viability and hair growth capacity |
| CN110585076A (en) * | 2019-09-26 | 2019-12-20 | 广州艾蓓生物科技有限公司 | Prebiotics and probiotic composition, and preparation method and application thereof |
| CN112175064A (en) * | 2020-10-12 | 2021-01-05 | 珠海乐维再生医学科技有限公司 | Preparation method of KGF protein exosome, KGF protein exosome dry powder, application and hair care agent |
| CN113304065A (en) * | 2021-05-08 | 2021-08-27 | 广州宝思利生物科技有限公司 | Scalp care composition and application thereof |
| CN113846046A (en) * | 2021-10-22 | 2021-12-28 | 科丝美诗(中国)化妆品有限公司 | Dendrobium-derived exosome-like vesicle, preparation method thereof and application thereof in alopecia improvement |
| CN114306432A (en) * | 2021-12-21 | 2022-04-12 | 广州远想生物科技股份有限公司 | Plant exosome composition with anti-alopecia and hair-growing functions and preparation method thereof |
| CN114191365A (en) * | 2021-12-31 | 2022-03-18 | 盈馨堂(武汉)商贸有限公司 | Anti-hair-loss and hair-growing essence and preparation method thereof |
| CN114392303A (en) * | 2022-02-17 | 2022-04-26 | 浙江卫未生物医药科技有限公司 | Exosome mixture for treating alopecia areata |
| CN115300404A (en) * | 2022-04-02 | 2022-11-08 | 苏州绿叶日用品有限公司 | Essence for hair and preparation method thereof |
| CN115054566A (en) * | 2022-06-29 | 2022-09-16 | 四川语安生物科技有限公司 | Scalp aging resisting composition and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 一种功能性酸奶的研制及其抗氧化活性;陈玉胜;陈全战;;江苏农业科学(第14期);第221-226页 * |
| 鲜人参中发现肿瘤免疫调节新物质;中国社区医师(第36期);第36页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116036196A (en) | 2023-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN116036196B (en) | Anti-hair loss hair-nourishing essence and preparation method thereof | |
| CN102349913B (en) | High concentration glycerol-type minoxidil tincture and preparation method thereof | |
| CN113908091A (en) | Anti-hair loss and hair-nourishing composition and preparation method and application thereof | |
| CN110064039B (en) | Anti-hair loss and hair-nourishing composition and application thereof | |
| CN117379465B (en) | Hair-growing and nourishing composition and preparation method and application thereof | |
| CN113750014A (en) | Anti-hair loss and hair-nourishing composition and preparation method and application thereof | |
| CN116370525A (en) | Plant fermentation composition for preventing hair loss and growing hair as well as preparation method and application thereof | |
| KR20210059144A (en) | Composition for preventing hair loss and promoting hair growth comprising an extract of Corylus heterophylla and Helianthus tuberosus, and method for manufacturing thereof | |
| TWI620567B (en) | External composition for skin containing ginsenoside rf | |
| CN116036018A (en) | Foam type hair growth liquid and preparation method thereof | |
| CN115887319A (en) | Skin-nourishing, hair-loss-preventing and hair-consolidating alpine plant composition, essence thereof and preparation method | |
| CN114732751A (en) | Anti-dropping essence containing pyrrolidinyl diaminopyrimidine oxide | |
| US9126056B2 (en) | Method of promoting hair growth | |
| EP4183404A1 (en) | Composition for preventing hair loss or promoting hair growth comprising extract of above-ground part of carrots | |
| CN107260656A (en) | Desonide cream and preparation method thereof | |
| CN116492242A (en) | Alopecia preventing composition, preparation method and application thereof | |
| WO2021157753A1 (en) | Composition for preventing hair loss and promoting hair regrowth | |
| JPS61137820A (en) | Male hormone blended agent for application | |
| CN115400049B (en) | Hair care composition containing ganoderma sinense extract and application thereof | |
| CN118121522B (en) | Hair-loss-preventing and hair-nourishing shampoo composition, and preparation method and application thereof | |
| CN116459176B (en) | Ginsenoside-containing composition, daily chemical and application | |
| CN113750013B (en) | Anti-hair-loss hair-growing composition and preparation method and application thereof | |
| EP2465581B1 (en) | Non-therapeutic use of a composition for promoting hair growth | |
| CN117298015A (en) | Anti-hair loss shampoo and preparation method thereof | |
| KR20220130708A (en) | Composition for preventing hair loss and promoting hair growth |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |