CN113750013B - Anti-hair-loss hair-growing composition and preparation method and application thereof - Google Patents
Anti-hair-loss hair-growing composition and preparation method and application thereof Download PDFInfo
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- CN113750013B CN113750013B CN202111197007.3A CN202111197007A CN113750013B CN 113750013 B CN113750013 B CN 113750013B CN 202111197007 A CN202111197007 A CN 202111197007A CN 113750013 B CN113750013 B CN 113750013B
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Abstract
The invention provides an anti-hair loss hair-growing composition, and a preparation method and application thereof. The hair-loss preventing hair-growing composition comprises: glycyrrhizin, boerduo leaf extract and oleanolic acid. The three components cooperate and synergistically, so that the activity of 5 alpha-reductase is effectively inhibited, the conversion of testosterone into DHT by 5 alpha-reductase is avoided to a great extent, and the problems of rapid entry into the retrograde phase, micromation, hair loss and the like caused by the action of high-activity DHT on hair follicles after the combination of the high-activity DHT and an androgen receptor are avoided; meanwhile, the hair loss prevention and hair growth promotion composition can also inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles, so that the purposes of preventing hair loss and promoting hair growth are finally achieved.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-hair-loss hair-growing composition, and a preparation method and application thereof.
Background
Hair is an accessory of human body, is an important component of human body, and has protection, modification and other physiological functions. And alopecia is a skin disease characterized by hair loss. Hair loss causes sparse hair or premature baldness, which can affect the appearance, brings much trouble and pain to patients, affects the normal work and life of people, and even destroys the mental health of patients, hits the self-confidence of people, and healthy maintenance of hair to prevent hair loss is an urgent problem for people to solve.
In the case of alopecia, however, one of the biggest causes is abnormal secretion of sebaceous glands. For abnormal sebaceous gland secretion, medicine is generally aimed at androgen excess, and estrogen regulation is increased or androgen secretion is reduced by using some drugs for abnormal sebaceous gland secretion caused by androgens, such as cimetidine, and 5 alpha-reductase is inhibited by using finasteride, dutasteride and irinotecan. However, the use of regulatory hormonal drugs has different injuries to the body. Therefore, the safety of the 5α -reductase inhibitory drugs of the prior art has yet to be improved. And for nursing oily skin, the skin is mainly cleaned thoroughly, the secretion of grease is controlled, and the skin is in a water-oil balance state as much as possible. Meanwhile, the anti-inflammatory and antibacterial effects are achieved, and skin diseases such as acne and seborrheic dermatitis can be effectively prevented and controlled.
CN105232410a discloses a shampoo product for controlling oil and preventing hair loss and a preparation method thereof, and the product comprises the following substances: lauroyl wheat amino acid potassium, cocamidopropyl betaine, soapberry pericarp extract, camellia seed extract, gleditsia sinensis lam extract, ginseng root extract, kuh-seng root extract, cowherb seed extract, mulberry leaf extract, garden balsam stem extract, licorice root extract, safflower extract, rheum palmatum root extract betaine, panthenol, allantoin, hydrolyzed collagen, hydroxypropyl guar hydroxypropyl trimethyl ammonium chloride, magnolia flower oil, a preservative and water. The oil-controlling hair-loss-preventing shampoo product can reduce scalp grease secretion and hair loss, but has poor effects in preventing alopecia and controlling skin diseases such as acne, seborrheic dermatitis and the like.
CN113116960a discloses a composition for treating alopecia and a preparation method thereof, and the traditional Chinese medicine composition is prepared from the following raw materials: water extract, ethanol extract, VC1, VE1, baicalin, oleanolic acid, glycyrrhizic acid, tea polyphenols, menthol and borneol. The invention can supplement nutrition required by hair roots to a certain extent, excite hair follicle activity and promote black hair growth, and is suitable for teenager alopecia and teenager female hair sparseness; however, the composition is also effective in preventing alopecia and controlling skin diseases such as acne and seborrheic dermatitis.
Therefore, it is an important research in the art to develop a head care product that fundamentally solves and fundamentally prevents the problem of hair loss.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an anti-hair loss hair-growing composition, and a preparation method and application thereof. The anti-hair loss hair-growing composition achieves the effects of controlling oil, inhibiting inflammatory factors and resisting inflammation and inhibiting bacteria by inhibiting the activity of 5 alpha-reductase, can realize the water-oil balance of scalp and effectively restore the barrier function of scalp, thereby fundamentally solving and preventing the problem of hair loss.
To achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an anti-hair-growing composition comprising: glycyrrhizin, boerduo leaf extract and oleanolic acid.
In the invention, the glycyrrhizin, the Boerduo leaf extract and the oleanolic acid are matched with each other, so that the synergistic effect is realized, the activity of 5 alpha-reductase can be effectively inhibited, the conversion of testosterone into DHT by 5 alpha-reductase is avoided to a great extent, and the problems of rapid entry into a retrograde phase, micromation, hair loss and the like caused by the action of high-activity DHT on hair follicles after the combination of the high-activity DHT and an androgen receptor are avoided; meanwhile, the hair loss prevention and hair growth promotion composition can also inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles, so that the purposes of preventing hair loss and promoting hair growth are finally achieved.
In the invention, the glycyrrhizin can inhibit the occurrence of peptic ulcer, prevent premature graying and alopecia, and can obviously improve the anti-hair loss effect when being added into the hair care anti-hair loss and hair care composition for diminishing inflammation, inhibiting bacteria, resolving masses and relieving exterior syndrome; the Boerduo leaf extract activates the synthesis of skin beta-defensin (an endogenous antibacterial peptide), enhances the natural antibacterial system of the skin, reduces bacterial growth, inhibits inflammatory factors, fine pores and improves oily scalp; the oleanolic acid comprises oleanolic acid as main component, can inhibit 5 alpha-reductase activity so as to achieve the oil control effect, and can show the advantages of quick oil control effect, long oil control time and difficult repetition.
Preferably, the anti-hair loss hair-growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Boerduo leaf extract and 0.01-2 parts of oleanolic acid.
In the hair-loss preventing hair-growing composition, the content of glycyrrhizin is 0.01-1 part, for example, 0.01 part, 0.02 part, 0.05 part, 0.08 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like.
In the hair-loss preventing and hair-growing composition, the content of the Boerdos leaf extract is 1-3 parts, for example, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts and the like.
The content of oleanolic acid in the hair-growing composition is 0.01-2 parts, and may be, for example, 0.01 parts, 0.05 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.8 parts, 1 parts, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, etc.
Preferably, the hair-loss prevention hair-growing composition further comprises any one or a combination of at least two of Chinese angelica extract, chinese prickly ash fruit extract, lightyellow sophora root extract or weeping forsythiae capsule extract, preferably a combination of Chinese angelica extract, chinese prickly ash fruit extract, lightyellow sophora root extract and weeping forsythiae capsule extract.
In the invention, the angelica extract, the pricklyash peel extract, the radix sophorae flavescentis extract and the fructus forsythiae extract are additionally added into the hair loss prevention and hair growth composition, so that the capabilities of balancing scalp grease, reducing dandruff, sterilizing and inhibiting bacteria and preventing hair loss are further improved.
In the invention, the main component of the angelica extract is angelica polysaccharide, which gives nutrition to the scalp and improves the nutrition transportation of the scalp, thereby recovering the cell functions of scalp epidermis, dermis and subcutaneous tissue and realizing the healthy omnibearing care of the scalp; the Chinese prickly ash fruit extract has pungent and warm taste, warms middle energizer to relieve pain, kills parasites and relieves itching; matrine and kurarinone in the kuh-seng extract are used as antibacterial components in the hair-growing agent; the forsythol, forsythoside A and other components contained in the forsythia extract have good antibacterial effects, and comprise streptococcus, cholera solitary fungus, typhoid bacillus, diphtheria bacillus, escherichia coli, shigella dysenteriae, staphylococcus and the like.
Preferably, the anti-hair loss hair-growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Boerduo leaf extract, 0.01-2 parts of oleanolic acid, 5-10 parts of Chinese angelica extract, 1-5 parts of Chinese prickly ash fruit extract, 3-8 parts of kuh-seng extract and 3-8 parts of weeping forsythiae capsule extract.
In the hair loss preventing and hair growing composition, the content of the angelica sinensis extract is 5-10 parts, for example, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts and the like can be adopted.
The content of the pricklyash peel extract in the hair-growing composition is 1-5 parts, for example, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like.
In the hair loss preventing and hair growing composition, the content of the kuh-seng extracting solution is 3-8 parts, for example, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like can be adopted.
In the hair-loss prevention and hair-growing composition, the content of the weeping forsythiae capsule extract is 3-8 parts, and can be 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like.
Preferably, the hair-growing composition further comprises a solvent, wherein the solvent is a mixed solution of alcohol and water.
Preferably, the mass ratio of the alcohol to the water is 1 (1-2), which may be, for example, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, etc.
Preferably, the alcohol comprises any one or a combination of at least two of ethanol, isopentyl glycol, octyl glycol, propylene glycol, butylene glycol, hexylene glycol or glycerin, preferably butylene glycol and/or hexylene glycol.
In the invention, the mixed solution of the specific alcohol and the water in proper proportion is selected for dilution, and the self-performance of each functional component in the anti-hair-loss and hair-growing composition can be further improved, so that the oil control, anti-inflammatory and antibacterial capabilities of the composition are further improved, and the problem of alopecia can be better solved and prevented.
Preferably, the anti-hair loss hair-growing composition comprises the following components in percentage by mass: 0.01-1% of glycyrrhizin, 1-3% of Boerduo leaf extract, 0.01-2% of oleanolic acid, 5-10% of Chinese angelica extract, 1-5% of Chinese prickly ash fruit extract, 3-8% of kuh-seng extract, 3-8% of weeping forsythiae capsule extract, 10-30% of butanediol, 10-30% of hexanediol and the balance of water.
The content of glycyrrhizin is 0.01-1% based on 100% of the total weight of the hair-growth composition, and may be, for example, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.
The content of the Boerdosis leaf extract is 1-3% based on 100% of the total weight of the hair-growing composition, and may be 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, etc.
The oleanolic acid content is 0.01-2% based on 100% total weight of the hair-growing composition, and may be, for example, 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc.
The content of the radix Angelicae sinensis extract is 5-10%, for example 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, etc., based on 100% of the total weight of the hair growth promoting composition.
The content of fructus Zanthoxyli extract is 1-5%, for example, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc. based on 100% total weight of the hair growth promoting composition.
The content of radix Sophorae Flavescentis extract is 3-8%, for example 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, etc. based on 100% total weight of the hair growth promoting composition.
The fructus forsythiae extract is 3-8% based on 100% of the total weight of the hair-caring composition, and can be 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, etc.
The content of butanediol is 10-30% based on 100% of the total weight of the hair-growth composition, and may be, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc.
The content of hexanediol is 10-30% based on 100% of the total weight of the hair-growing composition, and may be, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc.
In a second aspect, the present invention provides a method for preparing the hair-setting preventing composition as in the first aspect, comprising: dissolving liquiritin, the Boerduo leaf extract and oleanolic acid, and filtering to obtain the hair-loss preventing composition.
Preferably, the preparation method of the hair-loss preventing hair-growing composition comprises the following steps: dissolving liquiritin, boerduo leaf extract, oleanolic acid, radix Angelicae sinensis extract, fructus Zanthoxyli extract, radix Sophorae Flavescentis extract and fructus forsythiae extract in mixed solution of alcohol and water, mixing, stirring, and filtering to obtain the hair loss preventing composition.
In a third aspect, the present invention provides the use of an anti-hair loss hair-growing composition according to the first aspect for the preparation of a cosmetic.
In a fourth aspect, the present invention provides a cosmetic comprising an anti-hair-growing composition according to the first aspect.
Preferably, the cosmetic comprises any one or a combination of at least two of shampoo, essence or conditioner.
Preferably, the anti-hair loss and hair growth composition is added in an amount of 1-20% of the total mass of the cosmetic, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20% and the like.
In the invention, the anti-hair-loss hair-growing composition (C2 for short) with different concentrations has different changes of inhibition ratios of 5 alpha reductase activity in cosmetics; wherein, in pure water, the C2 is expressed as low concentration and high inhibition, namely, the concentration of the C2 is 4-6wt%, and the inhibition rate of the activity of 5 alpha reductase is the highest and can reach more than 75%; in the mixed solution of water and alcohol, the concentration of C2 is 9-11wt% of the middle concentration, and the inhibition rate of the activity of 5 alpha reductase is highest and can reach more than 105%; after DPO is additionally added into the mixed solution of water and alcohol, the concentration of C2 is 14-16wt% of high concentration, the inhibition rate of the anti-hair-growing composition on the activity of 5α reductase is highest, and the inhibition rate can reach more than 185%, so that the effect basically equivalent to 100wt% of C2 is achieved.
Compared with the prior art, the invention has the following beneficial effects:
(1) The anti-hair-loss composition provided by the invention effectively inhibits the activity of 5 alpha-reductase through the synergistic combination of three effective components of liquiritin, the Boerduo leaf extract and oleanolic acid, so that the problems that the 5 alpha-reductase catalyzes testosterone to be converted into DHT, and then the high-activity DHT is combined with an androgen receptor and then acts on hair follicles to generate the effects of rapidly entering a withdrawal period, microminiaturizing, hair loss and the like are avoided to a great extent;
(2) The anti-hair loss composition can inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles through the synergistic combination of three active ingredients of liquiritin, boerduo tree leaf extract and oleanolic acid, so that the purposes of preventing hair loss and promoting hair growth are finally achieved;
(3) The hair loss prevention and hair care composition can prevent and improve the condition of hair loss, keep the cortex sanitary, ventilate, not greasy, astringe excessively hypertrophic sebaceous glands, has the effects of reducing sebum secretion and promoting blood circulation, and has the effects of cleaning and stimulating the hair which is shed.
Detailed Description
The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.
The sources of the components of the following examples and comparative examples are shown below:
the mass ratio of the Chinese angelica extract in the following examples and comparative examples is (5): 6) =5:3
Example 1
The embodiment provides an anti-hair-loss hair-growing composition, which comprises the following components in percentage by mass based on the total mass of the anti-hair-loss hair-growing composition of 100 percent:
the preparation method of the hair-loss prevention hair-growing composition comprises the following steps: dissolving liquiritin, the Boerduo leaf extract, oleanolic acid, the Chinese angelica extract, the Chinese prickly ash fruit extract, the lightyellow sophora root extract and the weeping forsythiae capsule extract in the mixed solution of alcohol and water according to the formula amount, mixing and stirring for 5min at 25 ℃, and filtering by adopting filter cloth with 500 meshes to obtain the alopecia preventing hair-growing composition.
Example 2
The embodiment provides an anti-hair-loss hair-growing composition, which comprises the following components in percentage by mass based on the total mass of the anti-hair-loss hair-growing composition of 100 percent:
the preparation method of the hair-loss prevention hair-growing composition comprises the following steps: dissolving liquiritin, the Boerduo leaf extract, oleanolic acid, the Chinese angelica extract, the Chinese prickly ash fruit extract, the lightyellow sophora root extract and the weeping forsythiae capsule extract in the mixed solution of alcohol and water according to the formula amount, mixing and stirring for 5min at 25 ℃, and filtering by adopting filter cloth with 500 meshes to obtain the alopecia preventing hair-growing composition.
Example 3
The embodiment provides an anti-hair-loss hair-growing composition, which comprises the following components in percentage by mass based on the total mass of the anti-hair-loss hair-growing composition of 100 percent:
the preparation method of the hair-loss prevention hair-growing composition comprises the following steps: dissolving liquiritin, the Boerduo leaf extract, oleanolic acid, the Chinese angelica extract, the Chinese prickly ash fruit extract, the lightyellow sophora root extract and the weeping forsythiae capsule extract in the mixed solution of alcohol and water according to the formula amount, mixing and stirring for 5min at 25 ℃, and filtering by adopting filter cloth with 500 meshes to obtain the alopecia preventing hair-growing composition.
Examples 4 to 7
Examples 4-7 provide four anti-hair loss hair-growing compositions, differing from example 1 in the absence of any one of the angelica sinensis extract, the pricklyash peel extract, the kuh-seng extract and the weeping forsythia extract, respectively, and the preparation method is the same as example 1; the anti-hair loss hair-growing composition comprises the following components in percentage by mass:
comparative example 1
This comparative example provides an anti-hair loss composition, which is different from example 1 in that liquiritin, boldo leaf extract and oleanolic acid are not added, and the preparation method is the same as that of example 1; the anti-hair loss hair-growing composition comprises the following components in percentage by mass:
comparative examples 2 to 4
Comparative examples 2 to 4 provide three anti-hair loss hair-growing compositions differing from example 1 in that only one component of glycyrrhizin, boldo leaf extract and oleanolic acid is contained; the anti-hair loss hair-growing composition comprises the following components in percentage by mass:
comparative examples 5 to 7
Comparative examples 5-7 provide three anti-hair-setting compositions, differing from example 1 in the absence of an example of one of glycyrrhizin, boldo leaf extract and oleanolic acid; the anti-hair loss hair-growing composition comprises the following components in percentage by mass:
test example 1
5 alpha reductase Activity inhibition Rate test
Test sample: the anti-hair loss hair-growing compositions prepared in examples 1 to 7 and comparative examples 1 to 7;
the testing method comprises the following steps: the method specifically comprises the following steps:
(1) Preparation of 5 a reduction crude enzyme:
the male SD rat is killed by 10% chloral hydrate abdominal cavity excessive injection, liver tissue is rapidly dissected and removed, redundant blood is washed away by normal saline, phosphate buffer (pH=7.4) is added according to the mass ratio of 1:5, magnetic beads are added into a homogenizer for homogenization, the homogenization is taken out and centrifuged for 10min at 4 ℃ 10000r/min on a refrigerated centrifuge, the supernatant is taken out and centrifuged once again, the supernatant is taken out as crude enzyme liquid, and the crude enzyme liquid is frozen at-20 ℃ for standby.
(2) Protein content determination:
the protein concentration in the crude enzyme solution is determined by using a protein quantitative kit, and the protein standard is diluted to a mass concentration of 0, 10, 20, 40, 80, 120, 160, 200 mug/mL series of standard protein solutions according to the experimental method provided by the kit. 200 mu L of G250 staining solution is added into each series of standard solutions, after being uniformly mixed, the mixture is placed at room temperature for 3-5min, the absorbance of the mixture is detected at the wavelength of 595nm by using an enzyme-labeled instrument, and a standard curve is drawn by taking the absorbance (Y) of protein as an ordinate and the mass concentration (X, mg/mL) as an abscissa. The crude enzyme protein mass concentration was calculated by the protein standard curve equation.
(3) Assay for 5 alpha reductase Activity inhibition Activity
The total reaction volume of the 5 alpha reductase catalytic reaction is 200 mu L,120mg/mL liver homogenate, 5mg/mL NADPH, 0.2mg/mL testosterone and samples with different concentrations to be tested.
Blank group assay: liver homogenate (120. Mu.L), 5mg/mL NADPH (60. Mu.L), 0.2mg/mL testosterone (10. Mu.L) and phosphate buffer (pH 7.4) (10. Mu.L) are added into a 96-well plate, absorbance A0 is measured after thorough mixing, absorbance A1 is measured after incubation at 37 ℃ for 60min, and NADPH change value delta A0 is obtained.
Sample group assay: respectively preparing to-be-detected liquid (2, 5, 10, 20, 30, 40 mg/mL) of to-be-detected samples with different concentrations, and respectively carrying out reaction to obtain the NADPH variation value delta An.
Positive group assay: the finasteride is used as a positive control medicine, whether the activity of the enzyme is inhibited or not is judged according to the method, and the inhibition rate is calculated preliminarily.
The calculation formula is I% = (Δan- Δao)/[ Δao×100%. Experiments were repeated 3 times, 3 replicates each.
The specific test results are shown in Table 1
(wherein, the code number C2 represents the hair-loss prevention composition, DPO represents diaminopyrimidine oxide, and the dilution is carried out by water until the total content reaches 100%):
TABLE 1
The test results in table 1 show that the inhibition rate of 100wt% of the hair loss prevention hair growth composition to 5 alpha reductase activity is over 188%, which fully shows that the synergistic combination of the active ingredients of glycyrrhizin, the boldo leaf extract and oleanolic acid can effectively penetrate into scalp and hair follicles, thereby effectively inhibiting 5 alpha reductase activity, greatly avoiding 5 alpha reductase to catalyze testosterone to be converted into DHT, and further avoiding the problems of rapid entry into the retrograde phase, micromation, hair loss and the like caused by acting on hair follicles after the combination of high-activity DHT and androgen receptor.
In addition, in a dilution experiment, the anti-hair loss and hair growth promoting composition provided by the invention has a variation relation of different concentrations of inhibition rate of 5 alpha reductase activity, which is presented by cosmetics in different diluents; wherein the anti-hair-growing composition shows low concentration and high inhibition in pure water, the concentration of C2 is below 5wt%, and the inhibition rate of the anti-hair-growing composition to 5 alpha reductase activity is highest and can reach more than 75%; in the mixed solution of water and alcohol, the concentration of C2 is about 10wt% of the medium concentration, and the inhibition rate of the hair loss preventing hair growth promoting composition to 5 alpha reductase activity is highest and can reach more than 105%; after DPO is additionally added into the mixed solution of water and alcohol, the concentration of C2 is about 15wt% of the high concentration, and the inhibition rate of the hair loss prevention hair growth composition on the activity of 5 alpha reductase is the highest and can reach more than 185%.
In conclusion, 100 weight percent of the components in the hair loss prevention and hair growth promoting composition have extremely obvious synergistic effect; however, in various multiples of the dilution solvent, the anti-hair-growth composition of the present invention not only exhibits concentration dependence, but also exhibits antagonism between the components in part by way of example or irregularly, which in turn results in a decrease in inhibition rate of 5α reductase activity in part of the examples. Therefore, the most suitable scheme is to adopt a mixed solvent of alcohol and water and additionally add diaminopyrimidine oxide, so that the inhibition rate of the activity of the 5 alpha reductase which is equivalent to that of 100 weight percent concentration can be achieved, and the defects and waste caused by excessive addition amount can be avoided.
Test example 2
Inflammatory factor inhibition test
Test sample: the anti-hair loss hair-growing compositions prepared in examples 1 to 7 and comparative examples 1 to 7;
the testing method comprises the following steps: human immortalized keratinocytes (HaCaT cells) in the dosing group and the blank group were stimulated with Lipopolysaccharide (LPS), respectively, and protein expression of the relevant inflammatory factors interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in the HaCaT cell supernatants of the dosing group pretreatment group and the control group was detected by ELISA.
HaCaT cells were routinely cultured in DMEM complete medium (1% diabody: penicillin 100U/mL and streptomycin 100. Mu.g/mL added) containing 10% (volume fraction) fetal bovine serum at 37℃in a 5% (volume fraction) CO2 saturated humidity incubator with liquid changes every 2-3 days for 3-7 days. Taking HaCaT cells in logarithmic growth phase, digesting the cells for 10min by using 0.25% trypsin-EDTA, washing the cells, adding a complete culture medium into the cell sediment, and gently beating into a cell suspension. The cell concentration was adjusted to 4X 10 with fresh culture medium 4 Cells were inoculated onto 24 well plates, incubated in a cell incubator for 24h, and LPS was added after the cells had adhered to about 60% -70%. Blank groups, lipopolysaccharide LPS 20 mug/mL stimulated 24h groups, pretreatment of different administration groups 24h were setThe +LPS 20 mug/mL is stimulated for 24 hours, each group is provided with 5 compound holes, after the stimulation is finished, cell supernatant of each hole is sucked and split into 1.5mL EP pipes, and the cells can be used immediately or frozen at-20 ℃ for standby. The supernatants were tested for the expression levels of TNF- α and IL-1β using ELISA kit (Jiangsu enzyme-labeled Biotech Co., ltd.).
The specific test results are shown in table 2:
TABLE 2
As shown in the test data of Table 2, the anti-hair loss composition has good inhibition effect on the generation of inflammatory factors, the content of inflammatory factors IL-beta is reduced to below 112, and the content of inflammatory factors TNF-alpha is reduced to below 110 after the composition is used. Fully illustrates that the invention can inhibit IL-1 beta and TNF-alpha 2 inflammatory factors over expressed in skin hair follicles by the synergistic combination of three active ingredients of liquiritin, boerduo tree leaf extract and oleanolic acid, and has anti-inflammatory effect.
Test example 3
Mouse experiment
Test sample: the anti-hair loss hair-growing compositions prepared in examples 1 to 7 and comparative examples 1 to 7;
the testing method comprises the following steps: c57 mice (male, 5 weeks old) were kept in an environment of 23+ -2deg.C and 54+ -10% humidity, and were allowed to eat and drink water. Except for the blank group, the backs of the mice are selected to be injected with medicines for subcutaneous injection, testosterone propionate is injected for 5 mg/kg.d, the medicines are injected after 4 weeks of modeling success, physiological saline is smeared on the model group and the blank group, 5% minoxidil tincture is smeared on the minoxidil group for 0.2mL/d, and an aqueous solution corresponding to 5wt% of composition C2 is smeared on the sample group for 1 time every day for 4 weeks.
(1) Final hair/vellus hair value ratio: after the observation is finished at the 8 th week of the experiment, the mice are sacrificed, the skin of the back alopecia area of each group of mice is flatly attached to a fixed plate after about 1cm multiplied by 1cm, paraffin section dewaxing, hematoxylin-eosin staining and dehydration sealing experiment operation are carried out, under the field of 100 times of microscope, morphological changes of hair follicles of the mice are observed under the condition of 3 non-overlapping fields on average, the lengths and the sizes of the hair follicles are measured by utilizing Image-Pro Plus, and the ratio (A/T) of the number of hair follicles in the growing period to the number of hair follicles in the resting period is counted;
(2) ELISA determination of Dihydrotestosterone (DHT) and 5α reductase content in skin tissue: preparing tissue homogenate from tissues obtained by dissecting each group of mice, measuring the DHT and 5 alpha-reductase content in each group of samples by using an Elisa kit according to the method on an ELISA kit specification, recording data, and obtaining the average value by using Excel;
(3) Immunohistochemical determination of TGF- β1 and TNF- α content in skin: each group of mice is obtained by taking 2.0cm multiplied by 0.8cm long strip skin, placing the long strip skin in normal saline, fully rinsing residual blood, spreading the long strip skin on filter paper, airing the long strip skin at room temperature, wrapping the long strip skin on tinfoil paper, performing immunohistochemical detection to determine the contents of TGF-beta 1 and TNF-alpha in the skin of the mice according to the using method of Anti-TGFB1 and TNF-alpha, recording data, using Excel, and obtaining the average value of the data;
the specific test results are shown in table 3:
(wherein, the anti-hair loss hair-growing composition is represented by a code number C2):
TABLE 3 Table 3
As can be seen from the test data in Table 3, after the anti-alopecia composition of the present invention is used, the ratio of the final hair/vellus hair value of the HE staining result of the back test tissue of the mice is obviously increased to more than 4; ELISA measures the significant decrease in the content of Dihydrotestosterone (DHT) and 5α reductase in skin tissue, and immunohistochemistry measures the significant decrease in the content of TGF- β1 and TNF- α in skin. Therefore, the synergistic combination of three effective components of the liquiritin, the Boerduo leaf extract and the oleanolic acid can effectively penetrate into scalp and hair follicles, effectively inhibit 5 alpha-reductase and improve hair follicle atrophy caused by DHT; simultaneously inhibit the over-expressed IL-1 beta and TNF-alpha inflammatory factors caused by the breeding of anaerobic acne bacillus in skin hair follicles, can realize the simultaneous treatment of both symptoms and root causes to play roles in controlling oil and resisting inflammation, radically improve hair follicle microcirculation, stimulate cell metabolism and radically solve the problem of alopecia.
The applicant states that the present invention is illustrated by the above examples as well as methods of making and using the same, but the present invention is not limited to, i.e., does not mean that the present invention must be practiced in dependence upon the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
Claims (13)
1. An anti-hair-setting and hair-growing composition, characterized in that the composition comprises: glycyrrhizin, boerduo leaf extract and oleanolic acid; the anti-hair loss hair-growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Boerduo leaf extract and 0.01-2 parts of oleanolic acid;
the glycyrrhizin is derived from a licorice root extract, and the mass percentage of the glycyrrhizin in the licorice root extract is more than or equal to 50%.
2. The hair-loss preventing and hair-growing composition according to claim 1, further comprising any one or a combination of at least two of an angelica extract, a pricklyash peel extract, a kuh-seng extract, or a weeping forsythia extract.
3. The anti-hair-extension composition according to claim 1, wherein the anti-hair-extension composition comprises a combination of an angelica extract, a pricklyash peel extract, a kuh-seng extract and a weeping forsythia extract.
4. The anti-hair-extension composition according to claim 1, wherein the anti-hair-extension composition comprises, in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Boerduo leaf extract, 0.01-2 parts of oleanolic acid, 5-10 parts of Chinese angelica extract, 1-5 parts of Chinese prickly ash fruit extract, 3-8 parts of kuh-seng extract and 3-8 parts of weeping forsythiae capsule extract.
5. The anti-hair extension composition according to claim 1, wherein the anti-hair extension composition further comprises a solvent.
6. The hair-setting and hair-growing composition according to claim 5, wherein the solvent is a mixed solution of alcohol and water.
7. The hair-setting and hair-growing composition according to claim 6, wherein the mass ratio of the alcohol to the water is 1 (1-2).
8. The anti-hair-setting composition according to claim 6, wherein the alcohol comprises any one or a combination of at least two of ethanol, isopentyl glycol, octanyl glycol, propylene glycol, butylene glycol, hexylene glycol, or glycerin.
9. The anti-hair-setting composition according to claim 6, wherein the alcohol is butanediol and/or hexanediol.
10. The hair-loss prevention and hair-growth composition according to claim 1, which comprises the following components in percentage by mass: 0.01-1% of glycyrrhizin, 1-3% of Boerduo leaf extract, 0.01-2% of oleanolic acid, 5-10% of Chinese angelica extract, 1-5% of Chinese prickly ash fruit extract, 3-8% of kuh-seng extract, 3-8% of weeping forsythiae capsule extract, 10-30% of butanediol, 10-30% of hexanediol and the balance of water.
11. The method for preparing an anti-hair-extension composition according to any one of claims 1 to 10, wherein the method for preparing an anti-hair-extension composition comprises: dissolving liquiritin, boerduo leaf extract, oleanolic acid, radix Angelicae sinensis extract, fructus Zanthoxyli extract, radix Sophorae Flavescentis extract and fructus forsythiae extract in mixed solution of alcohol and water, mixing, stirring, and filtering to obtain the hair loss preventing composition.
12. Use of the hair-loss preventing composition according to any one of claims 1 to 10 in the preparation of a cosmetic, the hair-loss preventing composition being added in an amount of 1 to 20% of the total mass of the cosmetic.
13. A cosmetic product characterized in that it comprises the hair-setting preventing composition according to any one of claims 1 to 10; the addition amount of the anti-hair loss hair-growing composition accounts for 1-20% of the total mass of the cosmetic; the cosmetic comprises any one or a combination of at least two of shampoo, essence or hair conditioner.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101862374A (en) * | 2010-06-12 | 2010-10-20 | 李宏 | Lotus plumule and new application of extractive thereof |
| CN105585601A (en) * | 2012-12-27 | 2016-05-18 | 株式会社林原 | Skin-Exterior Anti-Ageing Composition And Production Method Therefor |
| CN107108837A (en) * | 2014-10-31 | 2017-08-29 | 路博润先进材料公司 | For delivering activating agent to the thermoplastic polyurethane film of skin surface |
| CN112220719A (en) * | 2020-10-30 | 2021-01-15 | 花安堂生物科技集团有限公司 | Hair-blacking composition and preparation method and application thereof |
| CN113116960A (en) * | 2021-06-01 | 2021-07-16 | 山东中医药大学 | A Chinese medicinal composition for treating alopecia, and its preparation method |
-
2021
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101862374A (en) * | 2010-06-12 | 2010-10-20 | 李宏 | Lotus plumule and new application of extractive thereof |
| CN105585601A (en) * | 2012-12-27 | 2016-05-18 | 株式会社林原 | Skin-Exterior Anti-Ageing Composition And Production Method Therefor |
| CN107108837A (en) * | 2014-10-31 | 2017-08-29 | 路博润先进材料公司 | For delivering activating agent to the thermoplastic polyurethane film of skin surface |
| CN112220719A (en) * | 2020-10-30 | 2021-01-15 | 花安堂生物科技集团有限公司 | Hair-blacking composition and preparation method and application thereof |
| CN113116960A (en) * | 2021-06-01 | 2021-07-16 | 山东中医药大学 | A Chinese medicinal composition for treating alopecia, and its preparation method |
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