CN116370525A

CN116370525A - Plant fermentation composition for preventing hair loss and growing hair as well as preparation method and application thereof

Info

Publication number: CN116370525A
Application number: CN202310239116.XA
Authority: CN
Inventors: 梁钦智; 陈雅丽; 王炯超; 田伟
Original assignee: Best Beauty Biotechnology Zhejiang Co ltd
Current assignee: Best Beauty Biotechnology Zhejiang Co ltd
Priority date: 2023-03-07
Filing date: 2023-03-07
Publication date: 2023-07-04

Abstract

The invention discloses a plant fermentation composition for preventing hair loss and growing hair, a preparation method and application thereof, wherein plant components used by the plant fermentation composition only comprise angelica sinensis, fleece-flower root and biota orientalis leaves, and the plant fermentation composition is different from the common method that an extracting solution is directly adopted for taking medicines; and (3) mixing the enzymolysis extracting solutions, and then co-fermenting with lactobacillus to obtain the plant fermentation composition. Experiments prove that after the three plants are subjected to enzymolysis extraction and fermentation composite treatment, the contents of active ingredients of angelica polysaccharide, stilbene glucoside and biota tops flavone in the three plants are obviously improved, the sediment is fewer, the stability is obviously improved, and the sticky feeling is obviously reduced. Compared with the anti-hair-loss and hair-growing product mixed by the general plant extract, the plant fermentation composition has better anti-hair-loss effect, higher hair density for hair growth, only three plant components and better safety.

Description

Plant fermentation composition for preventing hair loss and growing hair as well as preparation method and application thereof

Technical Field

The invention relates to the technical field of hair loss prevention and hair growth, in particular to a plant fermentation composition for hair loss prevention and hair growth, and a preparation method and application thereof.

Background

With the increase of the life rhythm and the continuous increase of the working strength, the alopecia diseases such as androgenetic alopecia (seborrheic alopecia), alopecia areata and the like are continuously increased, and the trend of younger is shown. The alopecia can prevent the appearance and the appearance, bring great mental stress and psychological burden to the patient more easily, and further seriously affect the quality of life. In the alopecia treatment, the western medicines commonly used in clinic are minoxidil and finasteride, but the two medicines are easy to relapse after stopping taking, and can also cause side effects such as skin irritation, scalp itching, facial and hand hirsutism, dizziness, anorexia, tachycardia and the like. In addition, although hair transplantation has a certain therapeutic effect, the clinical application of the hair transplantation is limited due to the large surgical trauma and high cost and the need of multiple surgeries. The effect of non-drug treatment such as acupuncture is also not ideal.

In recent years, more and more research reports on treating alopecia by using plant extracts such as plants are presented, however, single Chinese herbal medicines have limited promotion effect on hair growth in vitro culture, and according to the complementary theory of traditional Chinese medicine, a Chinese herbal medicine compounding mode is adopted effectively. The existing external anti-hair-loss and hair-growing product for plants is added with more than ten plant components frequently, the cost is too high, and the composite safety of multiple components is unknown.

Disclosure of Invention

In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a preparation method of a plant fermentation composition for preventing hair loss and growing hair, plant raw materials used by the composition are only angelica sinensis, fleece-flower root and biota orientalis, after water extraction and enzymolysis treatment are carried out on the plant raw materials, enzymolysis liquid is mixed and co-fermented with probiotics to obtain the plant fermentation composition, and the preparation method is simple in steps and suitable for mass production; the second purpose of the invention is to provide a plant fermentation composition for preventing hair loss and growing hair, which has the advantages of remarkably improved content of active ingredients, improved stability, reduced sticky feel, better use feeling and obviously improved hair loss preventing and growing effect; the invention further aims at providing an application of the plant fermentation composition for preventing hair loss and growing hair, and the plant fermentation composition is used for preparing a hair loss preventing and growing product.

One of the purposes of the invention is realized by adopting the following technical scheme:

a preparation method of a plant fermentation composition for preventing hair loss and growing hair comprises the following steps:

1) Extracting radix Angelicae sinensis with water, adding enzyme for treatment, inactivating enzyme, filtering to obtain supernatant, and collecting radix Angelicae sinensis enzymatic hydrolysate; extracting Polygoni Multiflori radix and folium Platycladi with water, adding enzyme, inactivating enzyme, filtering to obtain supernatant, and collecting the composite enzymolysis solution of Polygoni Multiflori radix and folium Platycladi;

2) Mixing the radix Angelicae sinensis enzymolysis solution and the radix Polygoni Multiflori and folium Platycladi compound enzymolysis solution, inoculating microorganism, fermenting, filtering to obtain supernatant, and obtaining the plant fermentation composition.

Further, the mass ratio of one of the Chinese angelica and the fleece-flower root and the biota orientalis leaves to water is 1: (5-20), preferably the mass ratio of the plant to the water is 1:10. the mass ratio of the angelica, the fleece-flower root and the biota orientalis leaves is (0.5-2): (2-4): (4-6).

Further; the water extraction temperature is 85-90 ℃ and the water extraction time is 30-60 min.

Further, in the step 1), after the angelica is extracted by water, amylase is added to treat for 1 to 3 hours at the temperature of between 40 and 60 ℃, enzyme is inactivated for 20 to 60 minutes at the temperature of between 80 and 90 ℃, and supernatant fluid is collected by filtration to obtain the angelica enzymolysis liquid, and the starch is removed by adopting amylase because the angelica contains more starch; after the fleece-flower root and the biota orientalis are extracted by water, pectase is added for treatment for 1 to 3 hours at the temperature of 40 to 60 ℃, enzyme is inactivated for 20 to 60 minutes at the temperature of 80 to 90 ℃, and supernatant fluid is collected by filtration, and the quality of the fleece-flower root and the biota orientalis composite enzymolysis liquid is relatively high, so that pectin is removed by using pectase.

Further, in step 2), the fermentation conditions are: fermenting at 35-38 deg.c for 18-36 hr; wherein the inoculation amount of the co-fermentation microorganism is 2-4% of the mass of the enzymolysis liquid.

Further, the microorganism is lactobacillus and/or yeast. Lactic acid bacteria are preferred.

Further, in the step 2), the supernatant after fermentation and centrifugation is concentrated to the concentration of crude drugs (0.1-0.2) g/g, and the anti-hair loss and hair growth plant fermentation composition is obtained. Preferably, a preservative is required to be added to the plant fermentation composition.

The second purpose of the invention is realized by adopting the following technical scheme:

the plant fermentation composition for preventing hair loss and growing hair is prepared by the preparation method of the plant fermentation composition for preventing hair loss and growing hair.

The third purpose of the invention is realized by adopting the following technical scheme:

the application of the plant fermentation composition for preventing and growing hair is that the plant fermentation composition for preventing and growing hair is used for preparing a product for preventing and growing hair; wherein the mass percentage of the plant fermentation composition for preventing and growing hair in the product for preventing and growing hair is 3-5%.

Compared with the prior art, the invention has the beneficial effects that:

(1) The plant components used in the preparation method of the plant fermentation composition only comprise angelica, fleece-flower root and biota orientalis, and the preparation method is that the angelica is extracted by water alone and then treated by amylase to prepare the angelica enzymolysis liquid, unlike the common method in which the extraction liquid is directly adopted for taking medicines; extracting Polygoni Multiflori radix and biota orientalis She Yiqi with water, adding pectase to obtain composite enzymolysis liquid, mixing the enzymolysis liquid with the enzymolysis liquid, inoculating microorganism, and fermenting to obtain the plant fermentation composition. Experiments prove that after enzymolysis and fermentation composite treatment, the contents of active ingredients in three plants, namely angelica polysaccharide, stilbene glucoside and biota tops flavone, are obviously improved, and compared with a fermentation composition only subjected to fermentation, the stability is less, the stability is obviously improved, the sticky feeling is obviously reduced, and the use feeling is better. Compared with the anti-hair-loss and hair-growing product mixed by the general plant extract, the plant fermentation composition has better anti-hair-loss effect, higher hair density for hair growth, only three plant components and better safety.

(2) The plant fermentation composition can be added as one of the components of the anti-hair loss essence, and is added according to the mass percentage of 3-5%, after the external application of the essence, the number of hair loss of a subject is reduced and the hair density is obviously improved through experimental tests, so that the plant fermentation composition has good anti-hair loss and hair growth effects.

Drawings

FIG. 1 is a graph showing the average value of the hair loss count of the hair loss preventing and growing essence of example 1;

fig. 2 is a graph showing the average hair density of the hair loss preventing and hair growth promoting essence of example 1.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, the following embodiments or technical features may be arbitrarily combined to form new embodiments.

The plant materials selected by the plant fermentation composition for preventing hair loss and growing hair are angelica sinensis, fleece-flower root and biota orientalis. From the pharmacological analysis of the traditional Chinese medicine, the polygonum multiflorum and the angelica are taken as plant pairs, so that the effects of tonifying liver and kidney, nourishing blood and activating blood can be achieved. On one hand, the hair-nourishing tea can enrich blood, nourish viscera and skin and hair when blood vessels are full, and hair luster is not easy to fall off; on the other hand, the Chinese medicinal composition can promote blood circulation, smooth blood circulation, prevent stasis, nourish hair, and firm hair roots, and generate new hair. The stilbene glucoside is a quantitative detection index of polygonum multiflorum, and has the functions of resisting oxidation and scavenging free radicals. The Angelica sinensis polysaccharide (angelica sinensis polysaccharide, ASP) as one of the main components of Angelica sinensis with pharmacological activity has hematopoietic activity, antioxidant activity, immunoregulatory activity, antibacterial and antiinflammatory effects. The Chinese arborvitae twig has the effects of cooling blood, stopping bleeding, relieving cough, eliminating phlegm, dispelling wind-damp and eliminating swelling and toxin. In "Rihua": the juice is burnt, the hair is smeared, and the hair is blackened. It is known that the biota orientalis leaves can be used for treating the hair and beard diseases besides the common blood cooling and bleeding stopping effects. The folium Platycladi flavone is used as main effective component of folium Platycladi, and has effects of relieving cough and eliminating phlegm, relieving asthma, tranquilizing and relieving spasm, resisting pathogenic microorganism, and resisting oxidation. All three have the functions of antioxidation and anti-inflammatory, can effectively improve the scalp microenvironment, and can achieve the effect of blackening hair by jointly reducing and avoiding the damage of melanocytes.

Example 1

1) The mass ratio is 1:3:5, cleaning three plants of angelica sinensis, fleece-flower root and biota orientalis, cutting, and then respectively extracting by adopting a water extraction method, wherein the mass ratio of the plants to water is 1:10; extracting with water at 85deg.C for 30min to obtain radix Angelicae sinensis extractive solution, polygoni Multiflori radix extractive solution and folium Platycladi extractive solution;

2) Adding amylase into radix Angelicae sinensis extractive solution, treating at 50deg.C for 2 hr, inactivating enzyme at 85deg.C for 30min, filtering, and collecting supernatant to obtain radix Angelicae sinensis enzymolysis solution; amylase accounts for 0.1% of the mass of the Chinese angelica extract; mixing the extractive solutions, adding pectase, treating at 50deg.C for 2 hr, inactivating enzyme at 85deg.C for 30min, and filtering to obtain supernatant; pectase accounts for 0.2% of the total mass of the extractive solutions;

3) Mixing radix Angelicae sinensis enzymolysis solution and Polygoni Multiflori radix folium Platycladi compound enzymolysis solution, inoculating microorganism, fermenting at 37deg.C and 200rpm with baffle plate shake flask, sealing 8 layers of gauze to improve dissolved oxygen, fermenting for 24 hr, filtering to obtain supernatant, concentrating to crude drug concentration of 0.15g/g, and adding antiseptic to obtain plant fermentation composition for preventing alopecia and promoting hair growth; wherein the inoculation amount of the microorganism is 4% of the mass of the enzymolysis liquid, and the inoculated microorganism is lactobacillus.

Example 2

The preparation method of the plant fermentation composition for preventing hair loss and growing hair of the present embodiment is different from that of the embodiment 1 in that: the co-fermenting microorganism selected in the step 3) is yeast.

Example 3

1) The mass ratio is 0.5:2:4, cleaning three plants of angelica sinensis, fleece-flower root and biota orientalis, cutting, and then respectively extracting by adopting a water extraction method, wherein the mass ratio of the plants to water is 1:5, a step of; extracting with water at 85deg.C for 60min to obtain radix Angelicae sinensis extractive solution, polygoni Multiflori radix extractive solution and folium Platycladi extractive solution;

2) Adding amylase into radix Angelicae sinensis extractive solution, treating at 40deg.C for 3 hr, inactivating enzyme at 80deg.C for 20min, filtering, and collecting supernatant to obtain radix Angelicae sinensis enzymolysis solution; amylase accounts for 0.1% of the mass of the Chinese angelica extract; mixing the extractive solutions, adding pectase, treating at 40deg.C for 1 hr, inactivating enzyme at 80deg.C for 60min, and filtering to obtain supernatant; pectase accounts for 0.2% of the total mass of the extractive solutions of Polygoni Multiflori radix and folium Platycladi respectively;

3) Mixing radix Angelicae sinensis enzymolysis solution and Polygoni Multiflori radix folium Platycladi compound enzymolysis solution, inoculating microorganism, fermenting at 35deg.C and 200rpm with baffle plate shake flask, sealing 8 layers of gauze to improve dissolved oxygen, fermenting for 18 hr, filtering to obtain supernatant, concentrating to crude drug concentration of 0.1g/g, and adding antiseptic to obtain plant fermentation composition for preventing alopecia and promoting hair growth; wherein the inoculation amount of the microorganism is 2% of the mass of the enzymolysis liquid, and the inoculated microorganism is saccharomycete.

Example 4

1) The mass ratio is 2:4:6, cleaning three plants of angelica sinensis, fleece-flower root and biota orientalis, cutting, and then respectively extracting by adopting a water extraction method, wherein the mass ratio of the plants to water is 1:20, a step of; extracting with water at 90deg.C for 60min to obtain radix Angelicae sinensis extractive solution, polygoni Multiflori radix extractive solution and folium Platycladi extractive solution;

2) Adding amylase into radix Angelicae sinensis extractive solution, treating at 60deg.C for 3 hr, inactivating enzyme at 90deg.C for 20min, filtering, and collecting supernatant to obtain radix Angelicae sinensis enzymolysis solution; amylase accounts for 0.1% of the mass of the Chinese angelica extract; mixing the extractive solutions, adding pectase, treating at 60deg.C for 1 hr, inactivating enzyme at 90deg.C for 20min, filtering, and collecting supernatant to obtain composite enzymolysis solution of folium Platycladi; pectase accounts for 0.2% of the total mass of the extractive solutions of Polygoni Multiflori radix and folium Platycladi respectively;

3) Mixing radix Angelicae sinensis enzymolysis solution and Polygoni Multiflori radix folium Platycladi compound enzymolysis solution, inoculating microorganism, fermenting at 38deg.C and 200rpm with baffle shake flask, sealing 8 layers of gauze to improve dissolved oxygen, fermenting for 18 hr, filtering to obtain supernatant, concentrating to crude drug concentration of 0.2g/g, and adding antiseptic to obtain plant fermentation composition for preventing alopecia and promoting hair growth; wherein the inoculation amount of the microorganism is 3% of the mass of the enzymolysis liquid, and the inoculated microorganism is lactobacillus.

Comparative example 1

Comparative example 1 differs from example 1 in that: the comparative example 1 was not subjected to the enzymolysis of the step 2), i.e., the angelica sinensis extract, the fleece-flower root extract and the biota orientalis extract were directly mixed and then fermented to obtain a plant fermentation composition. The remaining components and steps were the same as in example 1.

Comparative example 2

Comparative example 2 is different from example 2 in that: comparative example 2 the plant fermentation composition was obtained by directly mixing the angelica sinensis extract, the fleece-flower root extract and the biota orientalis extract without the enzymolysis of step 2) and then fermenting. The remaining components and steps were the same as in example 2.

Comparative example 3

Comparative example 3 is different from comparative example 1 in that: the microorganism used in comparative example 3 was Bacillus subtilis. That is, comparative example 3 was compared with example 1, and inoculated co-fermenting microorganism was replaced without the enzymatic hydrolysis step.

Comparative example 4

Comparative example 4 differs from example 1 in that: the microorganism used in comparative example 4 was Bacillus subtilis.

Comparative example 5

Comparative example 5 is different from example 1 in that: comparative example 5 without fermentation in step 3), the Chinese angelica enzymatic hydrolysate and the composite enzymatic hydrolysate of the biota orientalis leaf are directly mixed to obtain the plant enzymatic composition.

Comparative example 6

Comparative example 6 differs from example 1 in that: comparative example 6 the plant extract composition was obtained by directly mixing the angelica sinensis extract, the fleece-flower root extract and the biota orientalis extract without the enzymolysis of step 2) and the fermentation of step 3).

Comparative example 7

Comparative example 7 differs from example 1 in that: comparative example 7 does not add angelica, i.e. the plant fermentation composition contains only fleece flower root and biota orientalis.

Comparative example 8

Comparative example 8 differs from example 1 in that: comparative example 8 does not add fleece-flower root, i.e. the plant fermentation composition contains only angelica sinensis and biota orientalis.

Comparative example 9

Comparative example 9 is different from example 1 in that: comparative example 8 does not add cacumen Platycladi, i.e., the plant fermentation composition contains only Angelica sinensis and Polygonum multiflorum.

Performance testing

The invention sets example 1, comparative example 5 and comparative example 6 as experimental group a; example 1, example 2, comparative example 4 were set as experimental group B; comparative example 1, comparative example 2, comparative example 3 were set as experimental group C; example 1, comparative example 7, comparative example 8 and comparative example 9 were set as experimental group D.

Among them, experiment group a (example 1, comparative example 5 and comparative example 6) was to investigate the effect of enzymolysis and fermentation on performance of one or two deficiency; experiment group B (example 1, example 2, comparative example 4) was designed to investigate the effect of fermentation of different species of microorganisms on performance. Experiment group C (comparative example 1, comparative example 2 and comparative example 3) in order to investigate the effect of fermentation of different kinds of microorganisms on performance without going through enzymolysis, the errors generated by the enzymolysis step were reduced. The combination of panel B and panel C was analyzed to obtain the optimal microorganism. Experiment group D (example 1, comparative example 7, comparative example 8 and comparative example 9) was to investigate the effect of absence of any one of Angelica sinensis, platycladus orientalis leaf and Polygonum multiflorum on the hair growth preventing effect.

1. Active ingredient content test of each group (data see Table 1)

a. Microbial growth (OD) ₆₀₀ )

The method comprises the following steps: the absorbance at 600nm of the sample was tested to characterize the growth of the microorganism.

Sample: fermentation broths before concentration in examples 1 to 2 and comparative examples 1 to 4.

b. Testing the content of the effective component

The method comprises the following steps: high performance liquid chromatography is used for detecting the contents of stilbene glucoside and biota orientalis flavone, and phenol sulfate method is used for detecting the content of polysaccharide.

Sample: examples 1 to 2 and comparative examples 1 to 6

TABLE 1 data on the content of active ingredients of each group

As can be seen from Table 1, in the experimental group A, the active ingredients of the composition of example 1 subjected to enzymolysis and fermentation are obviously higher than those of the same experimental group, while the content of angelica polysaccharide and stilbene glucoside in the composition of comparative example 1 not subjected to enzymolysis is reduced, which indicates that the enzymolysis can improve the content of angelica polysaccharide and stilbene glucoside, but has little influence on cacumen Platycladi flavone; the composition of comparative example 5, in which the content of angelica polysaccharide was greatly reduced and the content of biota orientalis flavonoid was also slightly reduced, was greatly affected by whether fermentation was performed or not, and the effect of fermentation on stilbene glucoside was not great. The composition of comparative example 6, which was not subjected to enzymolysis and fermentation, was only prepared by simply mixing three kinds of plant compositions, and the three kinds of active ingredients were the lowest. So the content of active ingredients can be improved to a greater extent through enzymolysis and fermentation, thereby improving the subsequent hair loss prevention and hair growth effects.

In both experimental group B and experimental group C, the active ingredients of the co-fermented microorganism group (comparative example 3 and comparative example 4) using Bacillus subtilis as the inoculation were lower than those of the same experimental group, particularly the content of angelicapolysaccharide and stilbene glycoside, even close to that of comparative example 6, indicating that Bacillus subtilis is not suitable for selection as a co-fermented microorganism, so that the co-fermented microorganism is preferably yeast and lactic acid bacteria.

2. Each group was tested for adhesion and stability (data see Table 2)

a. The stability test method comprises the following steps: the sample was placed in a 48 ℃ oven, left to stand for 7 days, and then taken out to return to room temperature, and the precipitation was observed (compare with comparative example 6).

b. The sticky sense testing method comprises the following steps: the samples were applied to the back of the hand and the feel of tackiness of the samples was evaluated by skin feel (compare with comparative example 6).

Sample: examples 1 to 2 and comparative examples 1 to 5

Table 2 sticky feel and stability of the compositions of the groups

Group of	Sticky feeling	Stability of
			Example 1	Descent down	No obvious precipitate
Example 2	Descent down	No obvious precipitate
			Comparative example 1	Descent down	Precipitation reduction (++)
Comparative example 2	Descent down	Reduction of precipitation (+
			Comparative example 3	Unchanged	Unchanged
Comparative example 4	Unchanged	Unchanged
			Comparative example 5	Unchanged	Unchanged

In comparative example 6, after mixing the three plant extracts, there was much precipitation after standing, indicating poor mixing stability of the extracts. As can be seen from table 2, in the experimental group a, the viscosity of the composition of example 1 subjected to enzymolysis and fermentation was reduced while there was no significant precipitation, while the viscosity of comparative example 1 subjected to only fermentation was unchanged, and the precipitation was reduced, and the viscosity of comparative example 5 subjected to only enzymolysis was unchanged and the stability was unchanged, which means that the effect of only enzymolysis and only fermentation on the liquid viscosity was not great, and the viscosity of the composition was reduced and the stability was significantly improved after the enzymolysis and fermentation were combined.

In the experimental group B and the experimental group C, the viscosity and stability of the inoculated co-fermented microorganism were not changed as compared with those of comparative example 6 by using Bacillus subtilis (comparative example 3 and comparative example 4), and the active material agglomeration stability was not good by using Bacillus subtilis fermentation as compared with comparative examples 1 and 2, so that Bacillus subtilis was not suitable for the microorganism selected as co-fermentation. While comparative example 1 has less sediment than comparative example 2, indicating that the stability of fermentation of lactic acid bacteria is higher and that lactic acid bacteria are more suitable for selection as co-fermented microorganisms.

3. 5 alpha-reductase inhibition test of each group (data see Table 3)

Preparation of 5α -reductase 5 male SD rats were sacrificed by humane after overnight fast without water deprivation. Taking out the prostate, cutting the prostate on an ice table to prepare a 5 alpha-reductase extract, and preserving the extract at a low temperature for standby.

5 alpha-reductase Activity assay: 0.5mL of phosphate buffer, 0.1mL of test solution, 200. Mu.L of testosterone solution (300 mg/L) and 200. Mu.L of NADPH solution (0.8 g/L) were taken, 0.5mL of 5. Alpha. -reductase extract was added, after 30min reaction at 37℃2.5mL of methylene chloride was added to stop the reaction and extract testosterone, and 0.25mL of propylhydroxybenzoate (100 mg/L) was added as an internal standard, and centrifugation was carried out at 3 000r/min for 10min. The upper aqueous phase was removed, about 1mL of the organic phase was removed and evaporated to dryness, and the residue was dissolved in 1.5mL of methanol to give 10. Mu.L and the residual testosterone was measured by high performance liquid chromatography. Simultaneously, a 5 alpha-reductase reaction tube and a 5 alpha-reductase blank tube are set.

Sample to be tested: examples 1 to 2 and comparative examples 1 to 9

5α -reductase inhibition rate= (R _{Sample of} -R _Reaction )/(R _{Blank space} -R _Reaction ) X 100%; wherein R is the ratio of the target peak area to the internal standard peak area. Positive control: 0.05mg/L finasteride.

TABLE 3 data on inhibition of 5α reductase for each group

The higher the 5. Alpha. Reductase inhibition rate, the better the anti-drop effect. As is clear from table 4, examples 1 to 2 were excellent in the anti-slip effect. Among them, example 1 was more effective, i.e., the co-fermented microorganism was preferably lactobacillus. The anti-drop effect of comparative examples 1 to 9 was lower than that of example 1, indicating whether the anti-drop effect was affected by the enzymolysis, fermentation, selection of fermenting bacteria, and combination of the Chinese medicinal materials in the fermented composition. In combination, three medicinal materials of angelica sinensis, fleece-flower root and biota orientalis are adopted for water extraction, then enzymolysis and fermentation are carried out, and the scheme of the traditional Chinese medicine fermentation composition adopting lactic acid bacteria has the optimal anti-drop effect.

4. Test for trying out essence crowd

The plant fermentation composition of example 1 was added to the serum at a ratio of 5% to prepare an anti-hair loss serum. The scalp care essence formulation is shown in table 4.

Table 4 scalp care essence formulation table

Volunteer selection criteria: a total of 22 volunteers were recruited, 19 of the tests were finally completed and 3 subjects were withdrawn halfway. The final complete testers were 16 females, 3 males, and an average age of 30.32+ -6.04 years. All completed subjects met the following inclusion criteria, exclusion criteria and withdrawal (shedding) criteria:

inclusion criteria: 1.18 to 60 years old, healthy men or women; 2. the length of the hair is between 5 and 40 cm; 3. the hair loss is excessive and the hair is slightly sparse, and the hair loss count is more than 10 according to a 60-time comb method; 4. no special hair-dressing treatment such as hair dyeing, hair waving and shaping is performed within 1 month; 5. the test procedure can be understood, voluntarily taking part in the test and signing a written informed consent.

Exclusion criteria: 1. pregnant or lactating women, or those who have a pregnancy preparation plan recently; 2. severe androgenic alopecia, alopecia areata, inflammatory scarring alopecia or other patients suffering from scalp/hair disorders; 3. patients suffering from mental or psychological disorders; or with long-term sleep, mood control disorders; 4. cosmetic with hair loss preventing effect or other products with such effect or hair growth promoting effect are used in about 3 months; 5. any drugs affecting hair growth are taken or topically applied in the last 6 months; 6. those who have received hair transplantation therapy; 7. hair curlers; 8. highly sensitive body constitution; 9. other clinical trials were enrolled in approximately 2 months; 10. clinical evaluations were considered unsuitable for the participants.

Exit criteria: 1. subjects were out of visit and actively asked to exit; 2. the compliance is poor, the product can not be used in time according to the quantity, and the return visit can not be performed according to the requirement; 3. violating a study protocol; 4. serious adverse reactions occurred throughout the test period.

Selecting volunteers, taking essence prepared from the plant fermentation composition of example 1 once a day in the morning and evening, applying the essence to the head, and massaging and absorbing the essence once in the morning and evening. Hair loss and hair density in volunteers were measured four weeks later, eight weeks later and twelve weeks later, respectively. Wherein, the change of the number of volunteers falling hair is detected by adopting a 60-time combing method, and the change of hair density is analyzed by adopting a dermatoscope photographing.

As can be seen from fig. 1 to 2, the essence prepared from the plant fermentation composition of example 1 was reduced in hair loss by 48% and increased in hair density by 8% after continuous use for 12 weeks, indicating a good hair loss preventing and hair growth promoting effect.

The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention are intended to be within the scope of the present invention as claimed.

Claims

1. The preparation method of the plant fermentation composition for preventing hair loss and growing hair is characterized by comprising the following steps of:

2. The method for preparing the anti-hair loss and hair growth plant fermentation composition according to claim 1, wherein the mass ratio of one of angelica sinensis and biota orientalis leaves to water is 1: (5-20); the mass ratio of the angelica, the fleece-flower root and the biota orientalis leaves is (0.5-2): (2-4): (4-6).

3. The method for preparing the anti-hair loss and hair growth plant fermentation composition according to claim 1, wherein in the step 1), the temperature of the water extraction of the angelica sinensis, the fleece-flower root and the biota orientalis is 85-90 ℃ for 30-60 min.

4. The method for preparing the plant fermentation composition for preventing hair loss and growing according to claim 1, wherein in the step 1), after the angelica is extracted by water, amylase is added for treatment for 1-3 hours at 40-60 ℃, enzyme is inactivated for 20-60 minutes at 80-90 ℃, and supernatant is collected by filtration to obtain angelica enzymatic hydrolysate; extracting fleece-flower root and biota orientalis leaves with water, adding pectase, treating at 40-60 deg.c for 1-3 hr, inactivating enzyme at 80-90 deg.c for 20-60 min, filtering and collecting supernatant to obtain composite enzymolysis liquid.

5. The method for preparing the anti-hair loss and hair growth plant fermentation composition according to claim 1, wherein in the step 2), the fermentation conditions are as follows: fermenting at 35-38 deg.c for 18-36 hr; wherein the inoculation amount of the co-fermentation microorganism is 2-4% of the mass of the enzymolysis liquid.

6. The method for preparing a plant fermentation composition for hair loss prevention and hair growth according to claim 1 or 5, wherein the inoculated co-fermentation microorganism is lactic acid bacteria and/or yeast.

7. The method for preparing the plant fermentation composition for preventing hair loss and growing hair according to claim 1, wherein in the step 2), the angelica enzymatic hydrolysate and the fleece-flower root and biota orientalis composite enzymatic hydrolysate are mixed and inoculated with microorganisms for co-fermentation, the supernatant is filtered and collected, and the concentration of the crude drug is (0.1-0.2) g/g, so as to obtain the plant fermentation composition.

8. A plant fermentation composition for preventing hair loss and growing hair, which is characterized by being prepared by the preparation method of the plant fermentation composition for preventing hair loss and growing hair according to any one of claims 1 to 7.

9. Use of the anti-hair loss and hair growth plant fermentation composition of claim 9 for preparing an anti-hair loss and hair growth product; wherein the mass percentage of the plant fermentation composition for preventing and growing hair in the product for preventing and growing hair is 3-5%.