CN115400049B - Hair care composition containing ganoderma sinense extract and application thereof - Google Patents

Abstract

The application belongs to the field of cosmetics, and discloses a hair care composition containing ganoderma lucidum extract and application thereof. The composition comprises the following raw materials in parts by weight: 0.001-5 parts of ganoderma lucidum extract, 0.01-10 parts of B vitamins, 0.0001-10 parts of acetyl tetrapeptide-3, 0.0001-2 parts of diaminopyrimidine oxide and 0.0001-2 parts of citrus fruit extract. The application combines the ganoderma lucidum extract with high adenosine content with the optimized B vitamins, acetyl tetrapeptide-3, diaminopyrimidine oxide and citrus fruit extract to form a high-efficiency and high-activity composition, can inhibit the growth of malassezia and the activity of 5 alpha-reductase, achieves multiple effects of controlling oil, resisting inflammation, relaxing sensitization, preventing alopecia and the like, comprehensively and comprehensively improves the phenomena of alopecia, greasy scalp, itchy scalp, dandruff and long acne on scalp, and is beneficial to the health of hair and scalp.

Description

Hair care composition containing ganoderma sinense extract and application thereof

Technical Field

The application belongs to the field of cosmetics, and particularly relates to a hair care composition containing ganoderma lucidum extract and application thereof.

Background

Hair roots in hair follicles, whose activity determines the growth laws and health of hair. The hair follicle is a complicated skin accessory organ, is a three-dimensional tubular structure formed by continuously forming epidermal cells, and is mainly divided into an inner root sheath and an outer root sheath, wherein the outer root sheath is equivalent to a basal layer and a thorn layer of epidermis, and the inner root sheath is directly adjacent to hair in the later period of hair growth to guide hair growth. Hair follicles can be divided into the hair bulb, isthmus and funnel from bottom to top. The hair bulb part is the enlarged part of the lower end of the hair follicle, and the core is the hair papilla with the dermis layer protruding in. The stromal cells within the hair papilla can synthesize and secrete several inducers, growth factors and receptors that play a critical role in the formation and regeneration of hair follicles.

With the factors of the accelerated life rhythm, the change of the work and rest law, the working pressure, the rich and greasy diet, the wide interaction and compensation, the environmental pollution, the excessive scalding and dyeing and styling of hair, the mental tension, certain diseases or medicines and the like, the scalp is caused to have disorder or dysfunction in the local nerve metabolism, the physiological function degradation or malnutrition of hair follicles are caused, and the alopecia symptoms are caused. At present, the alopecia symptoms of people in various fields are increased, and even part of the people is expanded to the young people. If attention is not paid to the alopecia problem, countermeasures are not timely taken, further development is possible, necrosis or shedding of scalp hair follicle structures is caused, and irreversible permanent alopecia is caused.

There are various types of common hair loss, such as: seborrheic alopecia, psychogenic alopecia, physical alopecia, chemical alopecia, and the like. Clinically, the most common type of hair loss is seborrheic (androgenic) hair loss, accounting for about 90% of the population suffering from hair loss. Men and women can develop diseases, and men are more common. Male prevalence of androgenetic alopecia was reported to be 21.3% in our country and 6.0% in females. Most patients develop a slow progressive decrease in hair density from puberty. The pathological characteristic changes are mainly: hair follicle micromation and hair shaft thinning; the hair follicle cycle is changed, the hair follicle in the catagen stage and the catagen stage is increased, and the hair follicle in the anagen stage is reduced; often accompanied by greasy scalp.

Androgenic alopecia has a relatively complex pathogenesis, and androgens are decisive factors. This is because 5α -reductase in scalp hair follicles can turn Testosterone (T) into Dihydrotestosterone (DHT) which is more active. After androgen (T and DHT) and androgen receptor (Androgenic Receptor, AR) bind, dermal Papilla Cells (DPC) in scalp hair follicles are stimulated to secrete transforming growth factor β1 (tgfβ1). TGF-beta 1 can transform hair follicle from growing phase to retrogressive phase in inner root sheath, inhibit proliferation of hair follicle cells, and lead to hair thinning and shedding. Since Dihydrotestosterone (DHT) has more than 5 times higher affinity for the Androgen Receptor (AR) than testosterone (T). Thus, inhibition of 5α -reductase to reduce androgen levels has become an important approach to treating male hair loss. By inhibiting high affinity DHT production, androgenetic alopecia can be effectively prevented and slowed down.

Another common hair loss in clinic is hair loss caused by an imbalance in the microbial environment of the scalp. Malassezia is one of the causes of hair loss and is also a major cause of dandruff. Malachite bacteria is a common fungus group, mainly grows in the stratum corneum of human scalp, takes sebum secreted by the scalp as a nutrient, and is a type of lipophilic conditional pathogenic fungus. The increase of scalp seborrhea can cause mass propagation of malassezia, dysbacteriosis of scalp microorganisms, severe skin inflammation, and various problems such as seborrheic alopecia, dandruff, pruritus, scalp sensitivity and the like.

Therefore, there is an urgent need for a composition effective in preventing hair loss, preventing necrosis or shedding of the hair follicle structure of the scalp, and preventing permanent hair loss.

Disclosure of Invention

In view of the above-mentioned drawbacks of the prior art, an object of the present application is to provide a hair care composition containing ganoderma lucidum extract and its use, which are used for solving the problems of the prior art.

To achieve the above and other related objects, the present application is achieved by the following technical means.

The application aims at providing a hair care composition containing ganoderma lucidum extract, which comprises the following raw materials in parts by weight:

in some embodiments, the weight of the ganoderma lucidum extract may be 0.001-1 part, 0.5-3 parts, or 2-5 parts. In a preferred embodiment, 0.1 parts, 0.5 parts, 1.5 parts, 3 parts and 5 parts.

In some embodiments, the B vitamins may be 0.01 to 3 parts by weight, 2 to 7 parts by weight, or 5 to 10 parts by weight. In a preferred embodiment, 1.1 parts, 1.5 parts, 1.9 parts, 1.85 parts, 0.86 parts.

In some embodiments, the acetyl tetrapeptide-3 may be present in an amount of 0.0001 to 3 parts by weight, or may be present in an amount of 2 to 7 parts by weight, or may be present in an amount of 5 to 10 parts by weight. In a preferred embodiment, 8 parts, 4 parts, 5 parts and 0.0003 parts.

In some embodiments, the diaminopyrimidine oxide may be present in an amount of 0.0001 to 2 parts by weight, or may be present in an amount of 0.1 to 1 part by weight, or may be present in an amount of 0.5 to 2 parts by weight. In a preferred embodiment, 0.5 parts, 2 parts, 1.5 parts, 0.01 parts and 0.05 parts.

In some embodiments, the citrus fruit extract may be present in an amount of 0.0001 to 2 parts by weight, or may be present in an amount of 0.1 to 1 part by weight, or may be present in an amount of 0.5 to 2 parts by weight. In a preferred embodiment, 1 part, 2 parts, 0.5 parts, 0.01 parts and 0.05 parts.

In certain embodiments, the composition comprises the following raw materials in parts by weight: 5 parts of ganoderma lucidum extract, 0.855 part of B vitamins, 0.003 part of acetyl tetrapeptide-3, 0.05 part of diaminopyrimidine oxide and 0.05 part of citrus fruit extract.

In certain embodiments, the B vitamins are selected from one or more of vitamin B3, vitamin B5, vitamin B6, vitamin B7.

Preferably, the vitamin B is a mixture of vitamin B3, vitamin B5, vitamin B6 and vitamin B7.

More preferably, the mass ratio of the vitamin B3, the vitamin B5, the vitamin B6 and the vitamin B7 is (0.0001-5): (0.001-5): (0.0001-1): (0.0001-1). Specifically, the mass ratio of the vitamin B3, the vitamin B5, the vitamin B6 and the vitamin B7 may be (0.1 to 5): (0.1-1): (0.005-1): (0.03-1).

Preferably, the vitamin B3 is nicotinamide.

Preferably, the vitamin B6 is one or more of pyridoxine hydrochloride, pyridoxal hydrochloride and pyridoxamine hydrochloride.

More preferably, the vitamin B6 is pyridoxine hydrochloride.

In certain embodiments, the citrus fruit extract is prepared by:

mixing the acid with the citrus fruit powder, and carrying out solid-liquid separation to obtain filtrate and filter residues;

concentrating the filtrate to obtain a concentrated solution A; mixing the filter residues with polyalcohol, carrying out solid-liquid separation, and concentrating to obtain a concentrated solution B;

mixing the concentrated solution A and the concentrated solution B to obtain the citrus fruit extract.

Preferably, the acid is selected from aqueous hydrochloric acid.

More preferably, the concentration of the aqueous hydrochloric acid solution is 10 to 30wt%. Specifically, the concentration of the aqueous hydrochloric acid solution may be 10 to 22wt%, may be 15 to 26wt%, or may be 20 to 30wt%. In a certain preferred embodiment 25wt%.

Preferably, the pH value of the mixed solution formed by mixing the acid and the citrus fruit powder is 1-4. Specifically, the pH may be 1 to 3 or 2 to 4. In a certain preferred embodiment, 3.

Preferably, the polyol is selected from the group consisting of aqueous butanediol solutions.

More preferably, the concentration of the aqueous polyol solution is from 30 to 50wt%. Specifically, the concentration of the aqueous polyol solution may be 30 to 38wt%, may be 35 to 45wt%, or may be 40 to 50wt%. In a certain preferred embodiment 40wt%.

Preferably, the mass ratio of the acid to the citrus fruit powder is (5-10): 1. specifically, the mass ratio may be (5 to 7): 1, may be (6 to 9): 1, may be (8 to 10): 1. in a certain preferred embodiment, it is 7:1.

preferably, the mass ratio of the filter residue to the polyol is (5-10): 1. specifically, the mass ratio may be (5 to 7): 1, may be (6 to 9): 1, may be (8 to 10): 1. in a certain preferred embodiment, it is 6:1.

preferably, the concentration is carried out at 60-65 ℃ to a relative density of 1.05-1.10. Specifically, the concentration is carried out at 60 ℃, and the concentration is carried out until the relative density is 1.10.

In certain embodiments, the ganoderma lucidum extract comprises ganoderic acid DM and adenosine. The content of the adenosine is 0.05-1 wt% based on the total mass of the ganoderma lucidum extract.

In some embodiments, the method for preparing the ganoderma lucidum extract comprises the following steps:

placing Ganoderma powder in extraction kettle, introducing supercritical CO ₂ And entrainer, extract, get extract;

and (3) carrying out two-stage decompression separation on the extract, and drying to obtain the ganoderma sinense extract.

The preparation method of the ganoderma lucidum extract can obtain high-content adenosine.

In some embodiments, the particle size of the purple ganoderma lucidum powder is 40-80 meshes.

In certain embodiments, the entrainer is ethanol or methanol.

Preferably, the concentration of the ethanol is 60-95%. More preferably, the concentration of the ethanol may be 60 to 75wt%, or 70 to 85wt%, or 80 to 95wt%. In a preferred embodiment, 75wt%.

Preferably, the concentration of the methanol is 60-95%. More preferably, the concentration of the methanol may be 60 to 75wt%, or 70 to 85wt%, or 80 to 95wt%. In a preferred embodiment, 80wt%.

Preferably, the extraction pressure is 20-50 MPa. More preferably, the extraction pressure may be 20 to 35Mpa, 30 to 45Mpa, or 40 to 50Mpa. In a preferred embodiment, 34MPa.

Preferably, the extraction temperature is 30 to 50 ℃. More preferably, the dynamic extraction temperature may be 30 to 42 ℃, 35 to 46 ℃, or 43 to 50 ℃. In a certain preferred embodiment, 50 ℃.

Preferably, the extraction time is 1.5 to 6 hours. More preferably, the time of the dynamic extraction may be 1.5 to 3.0 hours, or may be 2 to 5 hours, or may be 4 to 6 hours. In a preferred embodiment, 5h.

Preferably, the primary separation pressure is 8-10 MPa. More preferably, the pressure of the first-stage decompression separation is 8-8.8 Mpa, can be 8.5-9.2 Mpa, and can be 9.1-10 Mpa. In a preferred embodiment, the pressure is 8MPa.

Preferably, the primary separation temperature is 35-50 ℃. More preferably, the temperature of the primary pressure reduction separation may be 35 to 42 ℃, 40 to 48 ℃, or 44 to 50 ℃. In a certain preferred embodiment, 50 ℃.

Preferably, the secondary separation pressure is 4-6 MPa. More preferably, the pressure of the secondary pressure reduction separation is 4 to 4.8MPa, may be 4.6 to 5.2MPa, or may be 5.1 to 6MPa. In a preferred embodiment, the pressure is 6MPa.

Preferably, the secondary separation temperature is 30-45 ℃. More preferably, the temperature of the secondary pressure reduction separation may be 30 to 42 ℃, 33 to 40 ℃, or 35 to 45 ℃. In a certain preferred embodiment, the temperature is 45 ℃.

It is a second object of the present application to provide a process for preparing a composition as described above, comprising the steps of:

mixing the raw materials according to parts by weight to obtain the composition.

It is a further object of the present application to provide the use of a composition as described above for the preparation of a hair care product.

In certain embodiments, the hair care product includes at least one of a shampoo, a serum, a conditioner, a hair spray, and a hair mask, but is not limited to a shampoo, a serum, a conditioner, a hair spray, and a hair mask, and any form of hair care product is within the scope of the present application.

It is a fourth object of the present application to provide a hair care product comprising a composition as described above.

In certain embodiments, the composition is added in an amount of 0.0001 to 10wt% based on the total mass of the hair care product.

Preferably, the composition may be added in an amount of 0.0001 to 2wt%, 1 to 6wt%, or 5 to 10wt%.

The ganoderma lucidum is one of the special rare edible and medicinal fungi in China, has sweet smell, warm nature and no toxicity, is recorded in Chinese pharmacopoeia (2010), and has pharmacological effects of resisting tumor, resisting oxidation, inhibiting bacteria, resisting inflammation, easing pain, resisting ulcer, resisting radiation, protecting liver, detoxifying, regulating immunity and the like. Ganoderic acid DM in Ganoderma extract has strong inhibition effect on 5α -reductase activity, and the extract also has inhibition effect on type II 5α -reductase. The ganoderma sinense extract also contains adenosine, and the adenosine is an endogenous nucleoside spread over human cells, is not only an important energy carrier in life, but also an important active ingredient of organism metabolism, has anti-inflammatory effect, can effectively regulate blood circulation of capillaries, supplements nutrition and moisture, can help to promote hair growth, increases hair quantity, and has good efficacy on promoting hair follicle health. In addition, the ganoderma sinense extract also contains a plurality of active ingredients beneficial to human bodies, such as polysaccharide, sterol, amino acid and the like, can play a synergistic effect with adenosine, and better plays a role in hair care.

Acetyl tetrapeptide-3 is a bionic peptide consisting of four amino acids, which mimics the action of natural peptides in humans, stimulates cell proliferation and controls cell differentiation, participates in intercellular communication, and stimulates hair follicle development. Meanwhile, acetyl tetrapeptide-3 can accelerate the formation of external matrix proteins such as fibronectin, collagen III and VII, etc., thereby effectively improving the hair follicle structure and increasing the hair anchoring stability. Has no side effect even if being used for human body for a long time.

The diaminopyrimidine oxide is a water-soluble hair growth promoter similar to minoxidil, which is an anti-alopecia drug, has good inhibition effect on 5α -reductase, and can be used as hair conditioner and hair loss preventing hair tonic in cosmetics. The diaminopyrimidine oxide can act on deep structures of hair roots to increase hair volume in anagen phase, and to revitalize hair roots, thereby promoting healthy hair growth. It also helps to soften collagen, and prevent hair loss due to perifollicular fibrosis.

Vitamin B3 (nicotinamide), vitamin B5 (panthenol), vitamin B6 (pyridoxine hydrochloride) and vitamin B7 (biotin) are all nutritional ingredients beneficial to hair and help to maintain hair and scalp health. The vitamin is applied to a hair care formula, can resist inflammation and itching, control scalp sebum, promote hair growth and prevent hair loss, can repair scalp barriers, helps scalp preserve moisture content, reduces moisture loss, and maintains scalp and hair. In the active combination of the present application, multivitamins are preferred, which provide nutrition to the hair from various aspects and enhance the efficacy of the hair care product of the ganoderma lucidum extract.

Citrus fruit is a traditional analgesic herbal medicine derived from immature citrus fruit. While the traditional Chinese medicine of green tangerine peel has been 800 years old, the minority nationalities such as aquatic animals, miao nationality, tujia and the like still have the traditional Chinese medicine of green tangerine peel for relieving pain. In addition, the pericarp of citrus plant also contains monoterpene compound, sesquiterpene compound, oxygen-containing derivative, etc., and has broad-spectrum antibacterial effect.

Compared with the prior art, the application has the following beneficial effects:

1) The composition contains the ganoderma lucidum extract and the citrus fruit extract which are natural plant sources, can inhibit the activity of 5 alpha-reductase, and has adverse reaction far lower than that of 5 alpha-reductase inhibitors of chemical synthesis and microbial sources; in addition, the malassezia can inhibit the proliferation of malassezia and reduce the secretion of grease, and has good effects in the aspects of treating and preventing seborrheic dermatitis and the like.

2) The application combines the ganoderma lucidum extract with high adenosine content with the optimized B vitamins, acetyl tetrapeptide-3, diaminopyrimidine oxide and citrus fruit extract to form a high-efficiency and high-activity composition, can inhibit the growth of malassezia and the activity of 5 alpha-reductase, achieves multiple effects of controlling oil, resisting inflammation, relaxing sensitization, preventing alopecia and the like, comprehensively and comprehensively improves the phenomena of alopecia, greasy scalp, itchy scalp, dandruff and long acne on scalp, and is beneficial to the health of hair and scalp.

Detailed Description

Further advantages and effects of the present application will become apparent to those skilled in the art from the disclosure of the present application, which is described by the following specific examples.

Before the embodiments of the application are explained in further detail, it is to be understood that the application is not limited in its scope to the particular embodiments described below; it is also to be understood that the terminology used in the examples of the application is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the application. The test methods in the following examples, in which specific conditions are not noted, are generally conducted under conventional conditions or under conditions recommended by the respective manufacturers.

Where numerical ranges are provided in the examples, it is understood that unless otherwise stated herein, both endpoints of each numerical range and any number between the two endpoints are significant both in the numerical range. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. In addition to the specific methods, devices, materials used in the embodiments, any methods, devices, and materials of the prior art similar or equivalent to those described in the embodiments of the present application may be used to practice the present application according to the knowledge of one skilled in the art and the description of the present application.

In the following embodiments of the present application, the mass ratio is 7:1, mixing 25wt% of hydrochloric acid with citrus fruit powder, and filtering to obtain filtrate and filter residue; concentrating the filtrate at 60deg.C to relative density of 1.10 to obtain concentrated solution A; the mass ratio is 6:1 mixing filter residues with 40wt% butanediol, filtering, concentrating at 60 ℃ until the relative density is 1.10, and obtaining a concentrated solution B; mixing the concentrated solution A and the concentrated solution B to obtain the citrus fruit extract.

In the following examples of the present application, 100g of Ganoderma sinense was taken, pulverized to a particle size of about 50 mesh, and put into an extraction vessel, and supercritical CO was used ₂ As solvent, 75wt% methanol was used as entrainer, wherein the extraction conditions were: the extraction pressure is 34MPa, and the temperature is 50 ℃; the separation conditions are as follows: the pressure of the primary separator is 8MPa, the temperature is 50 ℃, the pressure of the secondary separator is 6MPa, and the temperature is 45 ℃; and (3) dynamically operating for 5 hours, obtaining a ganoderma lucidum extract from the primary separator and the secondary separator, and then drying to obtain the ganoderma lucidum extract. The adenosine content is 0.8wt% based on the total mass of the Ganoderma sinense extract.

TABLE 1 sources and parameters of raw materials

Examples 1 to 5

Referring to table 2, the ganoderma lucidum extract, vitamin B3, vitamin B5, vitamin B6, vitamin B7, acetyl tetrapeptide-3, diaminopyrimidine oxide, and green tangerine peel extract were mixed to obtain a hair care composition containing the ganoderma lucidum extract.

TABLE 2 (wt%)

Referring to table 3, the hair care composition of example 5 was mixed with water, pentanediol, and hydroxyethyl cellulose, respectively, to obtain a essence containing ganoderma sinense.

Comparative examples 1-4 were also set up and the formulation is shown in Table 3.

Comparative example 1 differs from example 5 in that: commercial adenosine was used in place of Ganoderma sinense extract, and the commercial adenosine was added in an amount of 0.04wt% with the other components as in example 5 to obtain an essence.

Comparative example 2 differs from example 5 in that: the essence was obtained in the same manner as in example 5, except that the green tangerine peel extract was not added.

Comparative example 3 differs from example 5 in that: the essence was obtained in the same manner as in example 5, except that the purple ganoderma lucidum extract was not added.

Comparative example 4 differs from example 5 in that: the essence is obtained in the same manner as in example 5 without adding the extracts of Ganoderma sinense and pericarpium Citri Reticulatae viride.

TABLE 3 (wt%)

Application example

In this application example, the essential liquids obtained in example 5 and comparative examples 1 to 4 were used for the investigation of the oil content, hair loss and sensory investigation, and the bacteriostasis test.

Experimental design based on the principle of parallel control, a test was performed for 4 weeks to verify the efficacy of the hair care compositions of the present application. The subjects maintained their normal washing procedure and applied the test essence formulation once a day, uniformly applying the test formulation (about 6 mL) to the scalp, massaging thoroughly for 2-5 minutes to absorption without rinsing.

Subjects were instrumentally tested and filled in a self-assessment questionnaire before the start of the experiment and at the end of the experiment (after 4 weeks), respectively.

Experimental data were statistically analyzed using SPSS. P <0.05 was considered to have significant differences and P <0.01 was considered to have very significant differences.

And comparing the same formula before and after use, and adopting paired t test or related sample rank sum test according to whether the net change value is subjected to normal distribution.

Net change value = post-product use measurement-pre-product use base measurement

Subject information:

the experiments recruited 5 groups of subjects, 30 or more, aged 34 to 65 years, meeting the volunteer selection criteria of the subjects. The experiment is completed by 165 subjects (112 men and 53 women), and the number of each group is not less than 30, so that the statistical analysis requirement is met. The experimental group is shown in table 4.

Table 4 subject grouping

1.1 human body test items

1.1.1 sebum assay

Before the start of the experiment and at the end of the experiment, CUTOMETER was usedThe DUAL MPA 580 test instrument separately detects the lipid content of the scalp of each subject. The results of the oil content of the scalp are shown in Table 5.

TABLE 5 scalp fat content (μg/cm) ² )

Note that: * Representing P <0.05.

From table 5, it can be seen that the subjects were able to significantly reduce the amount of scalp fat after using the example formulation for 4 weeks.

1.1.2 alopecia counts

Before the start of the experiment and at the end of the experiment, each subject was subjected to hair loss counting by TrichoScan software analysis using MoleMax HD dermoscope instrument, and the hair loss counting results are shown in table 5.

TABLE 6 alopecia count (mean.+ -. Standard deviation)

Note that: * Represents P<0.05， ^** Represents P<0.01。

As can be seen from table 6, the number of hair loss in the subjects was extremely significantly reduced (P < 0.01) after 4 weeks of use of the hair care composition of the present application.

1.1.3 sensory evaluation

Subjects were scored for improvement in hair problems after use of the serum using a questionnaire format. Each sensory index is fully divided into 5 points, obviously improved into 5 points, and not improved into 0 point. The statistical results are shown in Table 7.

TABLE 7 subject self-questionnaire results

From Table 7, it is clear that the hair care composition of the present application has the best effect of improving the scalp discomfort symptoms of the subject.

From tables 5, 6 and 7, it is understood that the hair care composition of the present application has multiple effects of controlling lipid secretion, promoting hair growth, removing dandruff, relieving itching, and preventing the occurrence of acne on the scalp.

1.2 minimum inhibitory concentration test

Excessive secretion of grease brings enough nutrients for malassezia, causes blockage of scalp hair follicles, and finally leads to alopecia.

The minimum inhibitory concentration test (agar dilution) of the essence on malassezia furfur (Malassezia furfur) was determined by the method described in the "disinfection technical Specification" (2002 edition of Ministry of health).

TABLE 8 antibacterial test results

Note that: colonies were completely inhibited as "-", otherwise as "+".

As is clear from Table 3, each of comparative examples 1, 2, 3 and 4 and example 5 inhibited malassezia furfur, and the effect of example 5 was evaluated to be more excellent in combination, and the minimum inhibitory concentration was 2.5X10 ⁴ . The composition of the application has synergistic effect, can effectively inhibit the propagation of malassezia furfur, and solves the problems of abnormal increase of dandruff, alopecia and the like caused by malassezia furfur infection.

1.3 5 alpha-reductase Activity inhibition assay

The experiment uses NADPH test method. The 5 alpha-reductase catalyzes the conversion of testosterone to dihydrotestosterone in the presence of reduced coenzyme II, and if a 5 alpha-reductase inhibitor is present, this reaction process is inhibited to some extent. Since reduced coenzyme II has characteristic absorption at 340nn wavelength, the characteristic absorption at 340nm wavelength of oxidized coenzyme II generated after participating in the reaction disappears. The measurement by an ultraviolet spectrophotometer can reflect whether a certain substance has an inhibition effect and the inhibition effect on 5 alpha-reductase.

TABLE 9.5 results of alpha-reductase inhibition assay

Test sample	5 alpha-reductase inhibition ratio (%)
		Positive control (finasteride)	46.74
Example 5	39.90
		Comparative example 1	28.00
Comparative example 2	37.55
		Comparative example 3	26.03
Comparative example 4	24.60

As is clear from Table 9, all of comparative examples 1, 2, 3 and 4 and example 5 inhibit 5α -reductase, and the effect of example 5 was evaluated to be more excellent, and the 5α -reductase inhibition rate was 39.90. The composition provided by the application has a synergistic effect, and can effectively inhibit 5 alpha-reductase activity, so that testosterone is inhibited from being converted into dihydrotestosterone, and the problem of androgenetic alopecia is solved in a targeted manner.

In the composition provided by the application, the diaminopyrimidine oxide has a good inhibition effect on 5 alpha-reductase, and ganoderic acid DM in the ganoderma lucidum extract can inhibit the activity of 5 alpha-reductase, reduce the activity of sebaceous glands, limit sebum secretion and reduce bacterial proliferation; the citrus fruit extract has strong broad-spectrum antibacterial effect, has good inhibition effect on pityrosporum, and is beneficial to reducing dandruff; acetyl tetrapeptide-3 stimulates cell proliferation and controls cell differentiation, participates in intercellular communication and stimulates hair follicle development. The composition organically combines natural plants and non-plant-derived raw materials through a scientific formula of diaminopyrimidine oxide, citrus fruit extract, ganoderma lucidum extract and acetyl tetrapeptide-3, inhibits the activity of 5 alpha-reductase, reduces the proliferation of pityrosporum, controls the secretion of sebaceous glands and the like, achieves the synergistic effect respectively, improves alopecia in multiple ways, remarkably reduces the quantity of alopecia of subjects, and has no uncomfortable symptoms after use.

The addition of the ganoderma lucidum extract in the composition provided by the application can further enhance the activity of the composition for inhibiting 5 alpha-reductase, thereby reducing the combination of dihydrotestosterone and hair follicle receptors and increasing the hair growth cycle; the inhibiting effect of the citrus fruit extract on malassezia is also improved, and the composition can effectively remove dandruff and has a certain antipruritic effect; the composition of the application can be applied to washing and caring products to effectively improve seborrheic alopecia and promote hair growth.

The above embodiments are merely illustrative of the principles of the present application and its effectiveness, and are not intended to limit the application. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the application. Accordingly, it is intended that all equivalent modifications and variations of the application be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.

Claims (7)

1. A hair care composition containing ganoderma lucidum extract is characterized by comprising the following raw materials in parts by weight:

the vitamin B is a mixture of vitamin B3, vitamin B5, vitamin B6 and vitamin B7, and the mass ratio of the vitamin B3 to the vitamin B5 to the vitamin B6 to the vitamin B7 is (0.1-5): (0.1-1): (0.005-1): (0.03-1);

the preparation method of the ganoderma lucidum extract comprises the following steps:

placing Ganoderma powder in extraction kettle, introducing supercritical CO ₂ And entrainer, extract, get extract;

and (3) carrying out two-stage decompression separation on the extract, and drying to obtain the ganoderma sinense extract.

2. A composition according to claim 1, wherein the citrus fruit extract is prepared by a process comprising:

mixing the acid with the citrus fruit powder, and carrying out solid-liquid separation to obtain filtrate and filter residues;

concentrating the filtrate to obtain a concentrated solution A; mixing the filter residues with polyalcohol, carrying out solid-liquid separation, and concentrating to obtain a concentrated solution B;

mixing the concentrated solution A and the concentrated solution B to obtain the citrus fruit extract.

3. The composition of claim 2 wherein the acid is selected from the group consisting of aqueous hydrochloric acid; the concentration of the hydrochloric acid aqueous solution is 10-30wt%;

and/or the pH value of the mixed solution formed by mixing the acid and the citrus fruit powder is 1-4;

and/or the polyol is selected from butanediol aqueous solution; the concentration of the butanediol aqueous solution is 30-50wt%;

and/or, the mass ratio of the acid to the citrus fruit powder is (5-10): 1, a step of;

and/or the mass ratio of the filter residue to the polyol is (5-10): 1, a step of;

and/or concentrating at 60-65 deg.c to alcohol content not higher than 30% and relative density of 1.05-1.10.

4. The composition of claim 1, wherein the entrainer is ethanol or methanol;

and/or the extraction pressure is 20-50 MPa;

and/or the extraction temperature is 30-50 ℃;

and/or the primary separation pressure is 8-10 MPa;

and/or, the primary separation temperature is 35-50 ℃;

and/or the secondary separation pressure is 4-6 MPa;

and/or the secondary separation temperature is 30-45 ℃.

5. A method of preparing a composition according to any one of claims 1 to 4, comprising the steps of:

mixing the raw materials according to parts by weight to obtain the composition.

6. Use of a composition according to any one of claims 1 to 4 in the preparation of a hair care product.

7. A hair care product comprising the composition according to any one of claims 1 to 4.