CN113908091A - Anti-hair loss and hair-nourishing composition and preparation method and application thereof - Google Patents
Anti-hair loss and hair-nourishing composition and preparation method and application thereof Download PDFInfo
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- CN113908091A CN113908091A CN202111198925.8A CN202111198925A CN113908091A CN 113908091 A CN113908091 A CN 113908091A CN 202111198925 A CN202111198925 A CN 202111198925A CN 113908091 A CN113908091 A CN 113908091A
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- hair
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- hair loss
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/008—Preparations for oily hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention provides an anti-hair loss and hair-growing composition and a preparation method and application thereof. The hair loss preventing and hair growing composition mainly comprises: liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside. The four components are mutually matched and synergized, so that the activity of the 5 alpha-reductase is effectively inhibited, the 5 alpha-reductase is greatly prevented from catalyzing testosterone to be converted into DHT, and the problems that the high-activity DHT is combined with an androgen receptor and then acts on hair follicles to generate the effect of rapidly entering a catagen, being miniaturized, falling off hairs and the like are solved; meanwhile, the anti-hair loss and hair-growing composition can also inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles, so that the aims of preventing hair loss and promoting hair growth are finally fulfilled.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-hair loss and hair-growing composition and a preparation method and application thereof.
Background
The hair is an accessory of the human body, is an important component of the human body, and has protection, decoration and other physiological functions. Alopecia is a skin disorder characterized by hair loss. The hair loss causes hair sparseness or premature balding, the beauty is influenced, much trouble and pain are brought to patients, normal work and life of people are influenced, even the psychological health of the patients is disabled, the self-confidence of the people is struck, and the hair loss prevention by the healthy maintenance of the hair is an urgent problem to be solved by the people.
In the case of alopecia, one of the largest causative factors is abnormal sebaceous gland secretion. In response to sebaceous gland dyssecretosis, generally medically in response to hyperandrogenism, the use of increasing estrogen regulation or the use of some drugs such as cimetidine tincture against androgens that cause sebaceous gland dyssecretosis, and the use of finasteride, dutasteride and epristeride to inhibit 5 α -reductase. However, the use of hormonal drugs has various physical injuries. Therefore, the safety of the 5 α -reductase inhibitory drugs of the prior art has yet to be improved. For nursing oily skin, the main purposes are that the skin is clean and thorough, the secretion of grease is controlled, and the skin is in a water-oil balance state as much as possible. Meanwhile, the anti-inflammatory and antibacterial work is well done, and the skin diseases such as acne and seborrheic dermatitis can be effectively assisted and prevented and controlled.
CN110025775A discloses a hair loss preventing and hair restoring essence for mild hair fixation and a preparation method thereof, wherein the hair loss preventing and hair restoring essence for mild hair fixation comprises the following raw materials: water, ethanol, stilbene glucoside, glycerol, angelica sinensis extract, aloe extract, ginseng extract, tea polyphenol, corn gluten amino acids, vitamin H, folic acid, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, vitamin B1, yeast polypeptides, cysteine, sodium benzoate, potassium sorbate, citric acid, linoleic acid, xylanase and polyp extract. According to the invention, linoleic acid, xylanase and hydroid extracts are adopted to synergistically improve the hair loss prevention rate and the hair growth promotion rate of the hair loss prevention and generation promotion shampoo for mild hair strengthening.
CN103446563A discloses a plant extract compound for preventing alopecia and inhibiting 5 alpha-reductase activity, belonging to a plant extract composition. The compound hair loss preventing plant compound comprises serenoa repens extract, waxberry bark extract, platycladus orientalis extract, Chinese angelica extract, rhizoma anemarrhenae extract, selfheal extract, ginger extract, radix sophorae flavescentis extract, jackfruit extract, schisandra chinensis extract and rhizoma kaempferiae extract, wherein the weight percentage content of each plant extract in the total weight of the hair loss preventing plant compound is 0.1-99%. The anti-hair loss plant compound is used for treating and improving the seborrheic alopecia symptoms caused by male androgen source.
Therefore, the development of a head care product for fundamentally solving the problem of hair loss is a research focus in the art.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an anti-hair loss and hair-growing composition, a preparation method and application thereof. The anti-hair loss and hair-growing composition achieves the effects of controlling oil and inhibiting inflammatory factors by inhibiting the activity of 5 alpha-reductase, further achieves the effects of resisting inflammation and inhibiting bacteria, can realize the water-oil balance of scalp, and effectively repairs the barrier function of scalp, thereby radically solving the problem of hair loss.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an anti-hair loss hair growth composition comprising: liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside.
In the invention, the liquiritin, the boldo tree leaf extracting solution, the oleanolic acid and the stilbene glucoside are mutually matched, so that a synergistic effect is achieved, the activity of 5 alpha-reductase is effectively inhibited, the 5 alpha-reductase is prevented from catalyzing testosterone to be converted into DHT to a great extent, and the problems that the high-activity DHT is combined with androgen receptors and then acts on hair follicles to generate the effect of rapidly entering a catagen stage, being miniaturized, falling hair and the like are avoided; meanwhile, the anti-hair loss and hair-growing composition can also inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles, so that the aims of preventing hair loss and promoting hair growth are finally fulfilled.
In the invention, the liquiritin can inhibit peptic ulcer and prevent premature graying and alopecia, and can remarkably improve the anti-hair loss effect while diminishing inflammation, inhibiting bacteria, dissipating stagnation and relieving exterior syndrome when being added into the hair-care, anti-hair loss and hair-nourishing composition.
In the invention, the Bordeaux leaves extract activates the synthesis of skin beta-defensin (an endogenous antibacterial peptide), enhances the natural antibacterial system of the skin, reduces bacterial growth, inhibits inflammatory factors, refines pores and improves oily scalp.
In the invention, oleanolic acid can inhibit the activity of 5 alpha-reductase so as to achieve the oil control effect, and has the advantages of quick oil control effect, long oil control time and difficulty in repetition.
In the invention, the main component of the stilbene glucoside comprises the stilbene glucoside, the name of the stilbene glucoside is 2,3,5,4' -tetrahydroxy stilbene-2-O-beta-D-glucoside, the stilbene glucoside belongs to polyhydroxy stilbene compounds, and the stilbene glucoside can permeate into hairy roots and has the effects of sterilization, bacteriostasis and oxidation resistance.
Preferably, the hair loss preventing and hair growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Bordeaux leaf extract, 0.01-2 parts of oleanolic acid and 0.1-0.5 part of stilbene glycoside.
In the hair loss preventing and hair growing composition, the content of liquiritin is 0.01-1 part, for example, 0.01 part, 0.02 part, 0.05 part, 0.08 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like.
In the hair loss preventing and hair growing composition, the content of the bordeaux mixture extract is 1 to 3 parts, and may be, for example, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, or the like.
The anti-hair loss and hair-growing composition may contain 0.01 to 2 parts of oleanolic acid, for example, 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2 parts thereof.
The content of stilbene glucoside in the hair loss preventing and growing composition is 0.1-0.5 part, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part and the like.
Preferably, the hair loss preventing and hair nourishing composition further comprises angelica sinensis extract, pepper fruit extract, sophora flavescens extract and forsythia suspensa extract.
In the invention, the anti-hair-loss and hair-nourishing composition is additionally added with an angelica sinensis extracting solution, a zanthoxylum bungeanum fruit extracting solution, a sophora flavescens extracting solution and a fructus forsythiae extracting solution, so that the capabilities of balancing scalp grease, reducing dandruff, sterilizing and inhibiting bacteria and preventing hair loss are further improved.
The main component of the angelica sinensis extract is angelica sinensis polysaccharide, which endows nutrient substances to the scalp and improves the nutrition transportation of the scalp, thereby recovering the functions of scalp epidermis, dermis and subcutaneous tissue cells and realizing the comprehensive health care of the scalp; the Chinese prickly ash fruit extracting solution is pungent and warm in nature and taste, warms the middle-jiao to relieve pain, kills parasites and relieves itching; matrine and kurarinone substances in the radix sophorae flavescentis extract are used as antibacterial ingredients in the hair restorer; the forsythia suspense extract contains the components of forsythol, forsythin A and the like, and has good antibacterial effect, and comprises streptococcus, vibrio cholerae, typhoid bacillus, diphtheria bacillus, escherichia coli, dysentery bacillus, staphylococcus and the like.
Preferably, the hair loss preventing and hair growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of boldo leaf extract, 0.01-2 parts of oleanolic acid, 0.1-0.5 part of stilbene glycoside, 5-10 parts of angelica extract, 1-5 parts of pricklyash peel extract, 3-8 parts of radix sophorae flavescentis extract and 3-8 parts of fructus forsythiae extract.
The hair loss preventing and hair growing composition may contain 5 to 10 parts of the angelica sinensis extract, for example, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.
In the hair loss preventing and hair growing composition, the content of the pepper fruit extracting solution is 1 to 5 parts, and can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like.
In the hair loss preventing and hair restoring composition, the content of the sophora flavescens extract is 3 to 8 parts, and can be 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like.
In the hair loss preventing and hair growing composition, the content of the forsythia suspense extract is 3 to 8 parts, and for example, the forsythia suspense extract can be 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like.
Preferably, the hair loss prevention and growth promotion composition further comprises a solvent, wherein the solvent is a mixed solution of alcohol and water.
Preferably, the mass ratio of the alcohol to the water is 1 (1-2), and may be, for example, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, and the like.
Preferably, the alcohol comprises any one or a combination of at least two of ethanol, isoprene glycol, caprylyl glycol, propylene glycol, butylene glycol, hexylene glycol, or glycerol, preferably butylene glycol and/or hexylene glycol.
Preferably, the hair loss preventing and hair growing composition consists of the following components in percentage by mass: 0.01-1% of liquiritin, 1-3% of Bordeaux leaves extract, 0.01-2% of oleanolic acid, 0.1-0.5% of stilbene glycoside, 5-10% of Chinese angelica extract, 1-5% of Chinese prickly ash fruit extract, 3-8% of radix sophorae flavescentis extract, 3-8% of fructus forsythiae extract, 10-30% of butanediol, 10-30% of hexanediol and the balance of water.
The content of liquiritin is 0.01-1%, for example, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% or the like, based on 100% of the total weight of the hair loss preventing and growing composition.
The content of the bordeaux mixture extract is 1 to 3%, for example, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, etc., based on 100% by mass of the total hair loss preventing and growing composition.
The oleanolic acid content is 0.01 to 2% based on 100% of the total weight of the hair loss preventing and hair restoring composition, and may be, for example, 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, or the like.
The content of stilbene glucoside is 0.1-0.5%, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% and the like, based on 100% of the total mass of the composition.
The content of the angelica sinensis extract is 5 to 10%, for example, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, etc., based on 100% by mass of the hair loss preventing and growing composition.
The content of the extract of zanthoxylum bungeanum fruit is 1 to 5%, for example, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc., based on 100% of the total mass of the hair loss preventing and growing composition.
The content of the sophora flavescens ait extract is 3 to 8%, for example, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, etc., based on 100% of the total weight of the hair loss preventing and growing composition.
The content of the forsythia suspense extract is 3 to 8%, for example, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, etc., based on 100% of the total weight of the hair loss preventing and growing composition.
The content of the butylene glycol is 10 to 30%, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30% or the like, based on 100% by mass of the hair loss preventing and growing composition.
The content of hexanediol is 10 to 30%, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc., based on 100% by mass of the hair loss preventing and growing composition.
In a second aspect, the present invention provides a method for preparing the anti-alopecia hair-growing composition according to the first aspect, the method for preparing the anti-alopecia hair-growing composition comprising: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid and stilbene glucoside, and filtering to obtain the hair loss preventing and hair growing composition.
Preferably, the preparation method of the hair loss prevention and hair growth promotion composition comprises the following steps: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid, stilbene glucoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in mixed solution of alcohol and water, mixing and stirring, and filtering to obtain the hair loss preventing and nourishing composition.
In a third aspect, the present invention provides a use of the hair loss prevention and hair growth promotion composition according to the first aspect in the preparation of cosmetics.
In a fourth aspect, the present invention provides a cosmetic comprising the anti-hair loss and hair growth composition according to the first aspect.
Preferably, the cosmetic comprises any one of shampoo, cream shampoo, essence or hair conditioner or the combination of at least two of the shampoo, the cream shampoo, the essence and the hair conditioner.
Preferably, the addition amount of the hair loss prevention and hair growth promotion composition is 1 to 20% of the total mass of the cosmetic, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, etc., preferably 12 to 18%, and more preferably 15%.
Compared with the prior art, the invention has the following beneficial effects:
(1) the anti-hair loss and hair-growing composition disclosed by the invention effectively inhibits the activity of 5 alpha-reductase by synergistic compounding of four effective components, namely liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside, so that the problem that the high-activity DHT acts on hair follicles after being combined with androgen receptors to quickly enter a catagen stage, is miniaturized, and shed hair and the like is avoided to a great extent;
(2) the anti-hair loss and hair-growing composition disclosed by the invention can inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles by synergistic compounding of four effective components, namely liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside, so that the aims of preventing hair loss and promoting hair growth are finally achieved;
(3) the hair loss preventing and nourishing composition can prevent and improve the condition of hair loss, keep the cortex sanitary, breathable and non-greasy, and can be used for converging excessively fat sebaceous glands, reducing sebum secretion and promoting blood circulation, and has the effects of cleaning and stimulating fallen hair pores.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
The following examples and comparative examples have the following sources of the components:
in the embodiment, the weight ratio of the angelica sinensis extract is [ < 5 >: 3 >
Example 1
This embodiment provides an anti-alopecia hair-growing composition, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-alopecia hair-growing composition:
the preparation method of the anti-hair loss and hair-nourishing composition comprises the following steps: dissolving liquiritin, boldo tree leaf extracting solution, oleanolic acid, stilbene glycoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in a mixed solution of alcohol and water according to the formula ratio, mixing and stirring, and filtering to obtain the hair loss preventing and hair nourishing composition.
Example 2
This embodiment provides an anti-alopecia hair-growing composition, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-alopecia hair-growing composition:
the preparation method of the anti-hair loss and hair-nourishing composition comprises the following steps: dissolving liquiritin, boldo tree leaf extracting solution, oleanolic acid, stilbene glycoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in a mixed solution of alcohol and water according to the formula ratio, mixing and stirring, and filtering to obtain the hair loss preventing and hair nourishing composition.
Example 3
This embodiment provides an anti-alopecia hair-growing composition, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-alopecia hair-growing composition:
the preparation method of the anti-hair loss and hair-nourishing composition comprises the following steps: dissolving liquiritin, boldo tree leaf extracting solution, oleanolic acid, stilbene glycoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in a mixed solution of alcohol and water according to the formula ratio, mixing and stirring, and filtering to obtain the hair loss preventing and hair nourishing composition.
Examples 4 to 7
Examples 4 to 7 provide four hair loss preventing and hair growth promoting compositions, which are different from example 1 in that any one of an angelica extract, a zanthoxylum fruit extract, a sophora flavescens extract and a forsythia suspensa extract is absent, and the preparation method thereof is the same as example 1; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
comparative example 1
Comparative example 1 provides an anti-alopecia and hair-growing composition, which is different from example 1 in that liquiritin, boldo leaf extract, oleanolic acid and stilbene glucoside are not added, and the preparation method is the same as example 1; the anti-hair loss and hair-growing composition comprises the following components in percentage by mass:
comparative examples 2 to 5
Comparative examples 2 to 5 provide four hair loss prevention and hair growth promotion compositions, which are different from example 1 in that only one component selected from liquiritin, boldo leaf extract, oleanolic acid and stilbene glycoside is contained; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
comparative examples 6 to 8
Comparative examples 6 to 8 provide three hair loss prevention and hair growth promotion compositions, which are different from example 1 in that only two components of liquiritin, boldo leaf extract and oleanolic acid are mixed; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
comparative examples 9 to 11
Comparative examples 9 to 11 provide four hair loss prevention and hair growth promotion compositions, which are different from example 1 in that one component among liquiritin, boldo leaf extract, oleanolic acid is absent; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
test example 1
5 alpha reductase Activity inhibition assay
Test samples: the hair loss preventing and growing compositions prepared in examples 1 to 7 and comparative examples 1 to 11;
the test method comprises the following steps: the method specifically comprises the following steps:
(1) preparation of 5 α reduced crude enzyme:
the male SD rat is killed by 10% chloral hydrate and over-injected in the abdominal cavity, liver tissues are quickly dissected and taken out, excess blood is washed away by physiological saline, phosphate buffer (pH 7.4) is added according to the mass ratio of 1:5, magnetic beads are added to the mixture to be homogenized in a homogenizer, the homogenate is taken out and centrifuged for 10min at 10000r/min at 4 ℃ on a refrigerated centrifuge, the supernatant is taken out and centrifuged once again, the supernatant is crude enzyme liquid, and the crude enzyme liquid is frozen and stored for later use at-20 ℃.
(2) Protein content determination:
and (3) determining the protein concentration in the crude enzyme solution by using a protein quantitative kit, and diluting the protein standard substance into a series of standard protein solutions with the mass concentrations of 0, 10, 20, 40, 80, 120, 160 and 200 mu g/mL according to an experimental method provided by the kit. Adding 200 μ L of G250 staining solution into the above series of standard solutions, mixing, standing at room temperature for 3-5min, detecting absorbance at 595nm with microplate reader, and drawing standard curve with protein absorbance (Y) as ordinate and mass concentration (X, mg/mL) as abscissa. The crude enzyme protein mass concentration was calculated by the protein standard curve equation.
3)5 alpha reductase Activity inhibitory Activity assay
The total reaction volume of the 5 alpha reductase catalytic reaction is 200 mu L, and the total reaction volume is 120mg/mL liver homogenate, 5mg/mL NADPH, 0.2mg/mL testosterone and samples to be detected with different concentrations.
Blank group determination: adding 120 mu L of liver homogenate, 60 mu L of 5mg/mLNADPH, 10 mu L of 0.2mg/mL testosterone and 10 mu L of phosphate buffer (pH 7.4) into a 96-well plate, fully mixing, measuring the absorbance A0, incubating at the constant temperature of 37 ℃ for 60min, and measuring the absorbance A1 to obtain the NADPH change value delta A0.
Sample group determination: respectively preparing to-be-detected sample solutions (2, 5, 10, 20, 30 and 40mg/mL) with different concentrations, and respectively reacting to obtain the NADPH change value delta An.
And (4) positive group determination, namely determining whether the activity of the enzyme is inhibited or not by using finasteride as a positive control drug according to the method, and preliminarily calculating the inhibition rate.
The formula is calculated as I% (. DELTA.an-. DELTA.ao)/. DELTA.ao × 100%. The experiment was repeated 3 times, 3 replicates each time.
The specific test results are shown in Table 1
(wherein, the hair loss prevention and hair growth promotion composition is represented by the code C4, DPO represents diaminopyrimidine oxide, and both are diluted with water to make up to 100%, "-" represents the item not tested):
TABLE 1
The test results in table 1 show that the anti-hair loss and hair growth composition has an inhibition rate of 5 alpha reductase activity of over 180%, and fully shows that the anti-hair loss and hair growth composition can effectively penetrate into scalp and hair follicles through the synergistic compounding of four effective components, namely liquiritin, boldo leaf extract, oleanolic acid and stilbene glucoside, so that the activity of the 5 alpha reductase can be effectively inhibited, the 5 alpha reductase is prevented from catalyzing testosterone to be converted into DHT to a great extent, and the problems that the high-activity DHT acts on the hair follicles after being combined with androgen receptors to generate rapid entry into the catagen, miniaturization, hair loss and the like are solved.
In addition, in a dilution experiment, the anti-hair loss and hair growth promoting composition disclosed by the invention is suitable for cosmetics accounting for about 15% of the total mass of the cosmetics, not only can the inhibition rate of 5 alpha reductase activity be high, but also the defects and waste caused by excessive addition amount are avoided. Meanwhile, it is not difficult to find that the inhibition rate of the composition on the activity of the 5 alpha reductase can be further improved by additionally adding ethanol and/or diaminopyrimidine oxide at a low concentration (the addition amount of the C4 composition is 5-15%).
Test example 2
Inflammatory factor inhibition test
Test samples: the hair loss preventing and growing compositions prepared in examples 1 to 7 and comparative examples 1 to 11;
the test method comprises the following steps: lipopolysaccharide (LPS) is utilized to stimulate human immortalized keratinocytes (HaCaT cells) of an administration group and a blank group respectively, and protein expression of relevant inflammatory factors interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in supernatant of the HaCaT cells of a pretreatment group and a control group of the administration group is detected by ELISA.
HaCaT cells were routinely cultured in 10-containing mediaThe% (volume fraction) of fetal bovine serum was cultured in DMEM complete medium (supplemented with 1% diabody: penicillin 100U/mL and streptomycin 100. mu.g/mL) under conditions of 37 ℃ and 5% (volume fraction) CO2 in a saturated humidity incubator, with liquid change once every 2 to 3 days and passage once every 3 to 7 days. Taking HaCaT cells in logarithmic phase, digesting the cells with 0.25% trypsin-EDTA for 10min, cleaning the cells, adding a complete culture medium into the cell sediment, and gently blowing to obtain cell suspension. Fresh culture medium was used to adjust the cell concentration to 4X 104And (4) inoculating the cells to a 24-well plate, incubating for 24 hours in a cell incubator, and adding LPS after the adherence of the cells reaches about 60-70%. Setting a blank group, a Lipopolysaccharide (LPS) 20 mu g/mL stimulation 24h group, a pretreatment 24h + LPS 20 mu g/mL stimulation 24h group of different administration groups, setting 5 multiple holes in each group, sucking cell supernatant of each hole after stimulation is finished, subpackaging the cell supernatant into 1.5mL EP tubes, and immediately using or freezing at-20 ℃ for standby. The expression level of TNF-alpha and IL-1 beta in the supernatant was measured by ELISA kit method (Jiangsu enzyme-labeled Biotechnology Co., Ltd.).
The specific test results are shown in table 2:
TABLE 2
The test data in table 2 show that the hair loss prevention and hair growth promotion composition has a good inhibition effect on the generation of inflammatory factors, the content of the inflammatory factors IL-beta is reduced to below 125 after the hair loss prevention and hair growth promotion composition is used, and the content of the inflammatory factors TNF-alpha is reduced to below 126, so that the hair loss prevention and hair growth promotion composition fully shows that the hair loss prevention and hair growth promotion composition plays an anti-inflammatory role by the synergistic compounding of four effective components of liquiritin, Bordea leaf extract, oleanolic acid and stilbene glucoside, and simultaneously inhibits the IL-1 beta and TNF-alpha 2 inflammatory factors excessively expressed in skin hair follicles.
Test example 3
Mouse experiment
Test samples: the hair loss preventing and growing compositions prepared in examples 1 to 7 and comparative examples 1 to 11;
the test method comprises the following steps: c57 mice (male, 5 weeks old) were kept in an environment of temperature (23 + -2) ° C, humidity (54 + -10)% and free diet and drinking water. Except for a blank group, selecting the back of a mouse and injecting the drug subcutaneously, injecting testosterone propionate of 5 mg/kg. d, successfully modeling for 4 weeks and then administering, smearing normal saline on the model group and the blank group, smearing 5% minoxidil tincture of 0.2mL/d on the minoxidil group, smearing a corresponding 15 wt% of composition water solution on the sample group, 1 time every day and 4 weeks in a period.
(1) Ratio of terminal hair/vellus hair value: after the observation is finished in the 8 th week of the experiment, the mice are killed, the skin of the hair-shedding area at the back of each group of mice is taken and is flatly pasted on a fixed plate after being about 1cm multiplied by 1cm, paraffin section dewaxing, hematoxylin-eosin staining and dehydration mounting experiment operation are carried out, the morphological change of the hair follicles of the mice is observed under the 100-time microscope visual field and 3 non-overlapping visual fields on average, the length and the size of the hair follicles are measured by using Image-Pro Plus, and the ratio (A/T) of the number of the hair follicles in the growth phase to the number of the hair follicles in the resting phase is counted;
(2) ELISA assay of Dihydrotestosterone (DHT) and 5 α reductase content in skin tissue: dissecting the collected tissues of each group of mice to prepare tissue homogenate, measuring the content of DHT and 5 alpha-reductase in each group of samples by using an Elisa kit according to a method in an ELISA kit specification, recording data, and calculating the average value by using Excel;
(3) immunohistochemical determination of TGF-. beta.1 and TNF-. alpha.content in skin: taking 2.0cm multiplied by 0.8cm long-strip-shaped skin of each group of mice, placing the mice in physiological saline to fully rinse residual blood, spreading the residual blood on filter paper, drying the blood at room temperature, wrapping the mice with tin foil paper, carrying out an experiment for measuring the contents of TGF-beta 1 and TNF-alpha in the skin of the mice by immunohistochemical detection according to the using method of Anti-TGFB1 and TNF-alpha, recording data, and calculating the average value of the data by using Excel;
the specific test results are shown in table 3: (wherein, the hair loss prevention and hair growth promoting composition is represented by the reference numeral C4, "-" represents the item not tested):
TABLE 3
As can be seen from the test data in Table 3, the ratio of the terminal hair/vellus hair value of the HE staining result of the test area tissues on the back of the mouse is obviously increased to more than 4 after the anti-hair loss composition of the invention is used; the ELISA assay shows that the content of Dihydrotestosterone (DHT) and 5 alpha reductase in skin tissues is obviously reduced, and the immunohistochemical assay shows that the content of TGF-beta 1 and TNF-alpha in skin is obviously reduced.
Therefore, the synergistic combination of the liquiritin, the boldo leaf extracting solution, the oleanolic acid and the stilbene glucoside can effectively penetrate into the scalp and hair follicles, effectively inhibit 5 alpha-reductase and improve hair follicle atrophy caused by DHT (DHT); meanwhile, IL-1 beta and TNF-alpha inflammatory factors which are over-expressed due to the breeding of anaerobic bacteria acne bacillus in skin hair follicles are inhibited, the effects of controlling oil and resisting inflammation can be achieved by treating both symptoms and root causes, the microcirculation of the hair follicles is fundamentally improved, the cell metabolism is stimulated, and the hair loss problem is fundamentally solved.
The applicant states that the present invention is illustrated by the above examples to show the hair loss prevention and hair growth promotion composition of the present invention, and the preparation method and application thereof, but the present invention is not limited to the above examples, i.e. it is not meant that the present invention is implemented only by relying on the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (10)
1. An anti-alopecia hair-growing composition, comprising: liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside.
2. The hair loss prevention and hair growth composition of claim 1, wherein the hair loss prevention and hair growth composition comprises in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Bordeaux leaf extract, 0.01-2 parts of oleanolic acid and 0.1-0.5 part of stilbene glycoside.
3. The hair loss prevention and hair growth promoting composition according to claim 1 or 2, further comprising an angelica sinensis extract, a zanthoxylum bungeanum fruit extract, a sophora flavescens extract and a forsythia suspensa extract.
4. The anti-alopecia hair-growing composition according to any one of claims 1-3, wherein the anti-alopecia hair-growing composition comprises, in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of boldo leaf extract, 0.01-2 parts of oleanolic acid, 0.1-0.5 part of stilbene glycoside, 5-10 parts of angelica extract, 1-5 parts of pricklyash peel extract, 3-8 parts of radix sophorae flavescentis extract and 3-8 parts of fructus forsythiae extract.
5. The anti-alopecia hair-growing composition according to any one of claims 1 to 4, further comprising a solvent, wherein the solvent is a mixed solution of alcohol and water;
preferably, the mass ratio of the alcohol to the water is 1 (1-2);
preferably, the alcohol comprises any one or a combination of at least two of ethanol, isoprene glycol, caprylyl glycol, propylene glycol, butylene glycol, hexylene glycol, or glycerol, preferably butylene glycol and/or hexylene glycol.
6. The hair loss prevention and hair growth promoting composition according to any one of claims 1 to 5, wherein the hair loss prevention and hair growth promoting composition comprises the following components in percentage by mass: 0.01-1% of liquiritin, 1-3% of Bordeaux leaves extract, 0.01-2% of oleanolic acid, 0.1-0.5% of stilbene glycoside, 5-10% of Chinese angelica extract, 1-5% of Chinese prickly ash fruit extract, 3-8% of radix sophorae flavescentis extract, 3-8% of fructus forsythiae extract, 10-30% of butanediol, 10-30% of hexanediol and the balance of water.
7. The method of preparing the anti-alopecia hair-growing composition according to any one of claims 1-6, wherein the method of preparing the anti-alopecia hair-growing composition comprises: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid and stilbene glucoside, and filtering to obtain the hair loss preventing and hair growing composition.
8. The method of preparing the hair loss prevention and hair growth composition of claim 7, wherein the method of preparing the hair loss prevention and hair growth composition comprises: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid, stilbene glucoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in mixed solution of alcohol and water, mixing and stirring, and filtering to obtain the hair loss preventing and nourishing composition.
9. Use of the anti-alopecia hair-care composition according to any one of claims 1-6 for preparing a cosmetic.
10. A cosmetic comprising the hair loss preventing and hair growth promoting composition according to any one of claims 1 to 6;
preferably, the cosmetic comprises any one or combination of at least two of shampoo, cream shampoo, essence or hair conditioner;
preferably, the addition amount of the hair loss prevention and hair growth promotion composition accounts for 1-20%, preferably 12-18%, and more preferably 15% of the total mass of the cosmetic.
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