CN116035991A

CN116035991A - Plant extract composition with antiallergic and soothing effects and cosmetic

Info

Publication number: CN116035991A
Application number: CN202310053921.3A
Authority: CN
Inventors: 曹崇江; 何豫皖; 程抒劼; 刘诗琪; 徐莹
Original assignee: China Pharmaceutical University
Current assignee: China Pharmaceutical University
Priority date: 2023-02-03
Filing date: 2023-02-03
Publication date: 2023-05-02

Abstract

The invention discloses a plant extract composition with anti-allergy and soothing effects and a cosmetic, wherein the plant extract composition comprises a cogongrass rhizome extract, a dwarf lilyturf tuber extract and a purslane extract. Animal experiments show that the cogongrass rhizome extract as an active ingredient can reduce the skin inflammation desquamation condition, reduce scratching condition, relieve abnormal thickening condition of the epidermis layer and reduce the number of skin mast cells, and can be widely used in skin care products for relieving and resisting sensitization. Cell experiments show that the cogongrass rhizome can play a role in resisting inflammation and relieving skin irritation by being independently used, and the anti-inflammatory effect can be improved by being combined with the ophiopogon root extract and the purslane extract with similar effects. The combined extract is applied to gel cosmetic products, and the prepared products have transparent appearance, fresh and cool use feeling and good moisturizing effect.

Description

Plant extract composition with antiallergic and soothing effects and cosmetic

Technical Field

The invention relates to a plant extract composition and a cosmetic, in particular to a plant extract composition and a cosmetic with anti-allergy and relieving effects, and belongs to the technical field of traditional Chinese medicines and the technical field of cosmetics.

Background

Sensitive skin is a kind of skin which is easy to feel stinging, burning and the like under the stimulation of external physics, chemistry, cold and hot, and the like, and especially is most on the face. It is mainly characterized in that itching and stinging feeling occur after external stimulation, and phenomena such as dryness, desquamation, erythra and the like are easy to occur. According to the cosmetic classification rules and classification catalogues issued by the national drug administration, the "soothing" efficacy is interpreted as being beneficial to improving the state of skin irritation and the like. Raw materials derived from natural plants have a higher specific gravity in the cosmetic field and are increasingly favored by consumers due to the guidance of concepts such as green, mild, sustainable, etc.

The cogongrass rhizome, also called as the root of cymbidium, the root of cogongrass, the root of sweet grass, the sugarcane, etc., is the dry rhizome of cogongrass (Imperata cylindrical Beauv. Var. Major (Nees) C.E. Hubb.) of cogongrass of Gramineae, has sweet and cold taste, has the effects of cooling blood, stopping bleeding, clearing heat, promoting urination, etc. Bai Mao Gen is originally carried in Shen nong Ben Cao Jing (Shen nong's herbal), and is mainly used for treating febrile disease, polydipsia, hematemesis, dyspnea due to lung heat, difficult urination, edema, jaundice and the like. The cogongrass rhizome contains a plurality of chemical components such as polysaccharide, triterpene, flavone, coumarin, phenolic acid and the like, and modern pharmacological researches show that the cogongrass rhizome has wide biological activity, such as a plurality of pharmacological effects such as antioxidation, anti-inflammatory, anti-tumor, antibiosis, hemostasis and immunoregulation.

The application of cogongrass rhizome is mainly used in oral care products, hemostatic products, moisturizing products and the like at present. Chinese patent CN103385848A discloses a method for preparing a toning lotion with moisturizing, skin elasticity enhancing and wrinkle reducing effects, wherein lalang grass rhizome extract is compounded with a plurality of active ingredients. Chinese patent CN105640868A discloses a pearl amino acid moisturizing mask and a preparation method thereof, wherein cogongrass rhizome extract and other active ingredients are synergistic to act, and the moisturizing mask has the functions of relieving tiredness and darkness of skin after being decocted, moisturizing and brightening.

Chinese patent CN107184860A discloses a traditional Chinese medicine composition with the effects of relieving itching, resisting contact eczema and contact dermatitis, which is obtained by decocting 6 traditional Chinese medicine compounds including cogongrass rhizome with water, and can clear heat, cool blood, invigorate spleen, promote diuresis and cope with acute eczema. However, the effect is realized on the basis of the synergistic effect of the whole traditional Chinese medicine compound, and the oral administration mode is adopted. Although the cogongrass rhizome has long history of use as a medicinal and edible traditional Chinese medicine, the application of cogongrass rhizome extract on the skin surface alone is not reported at present, and the effect of relieving the effect and improving the skin irritation state is exerted.

Therefore, the cogongrass rhizome extract with the relieving effect and the skin stimulation improving effect has important significance.

Disclosure of Invention

The invention aims to: the object of the present invention is to provide a plant extract composition which can improve skin irritation and has an antiallergic and soothing effect; another object of the present invention is to provide a cosmetic containing the above plant extract composition; another object of the present invention is to provide a method for preparing the above cosmetic.

The technical scheme is as follows: the plant extract composition with the antiallergic and soothing effects comprises a cogongrass rhizome extract, a dwarf lilyturf tuber extract and a purslane extract. Preferably, in the plant extract composition, the mass ratio of the cogongrass rhizome extract to the dwarf lilyturf tuber extract to the purslane extract is 1:1:1.

As a further improvement of the above scheme, the cogongrass rhizome extract is prepared by the following method: reflux-extracting the dried and pulverized lalang grass rhizome powder with ethanol water solution under stirring, concentrating the filtrate under reduced pressure, and drying.

As a further improvement of the scheme, in the preparation method of the cogongrass rhizome extract, cogongrass rhizome powder is 60-80 meshes, the stirring reflux extraction time is 2-3 hours, the volume of the ethanol aqueous solution of the extract is 10-20 times of the mass of cogongrass rhizome powder, the extraction times are more than or equal to 2 times, and the temperature of reduced pressure concentration is 40-50 ℃.

In another aspect, the present invention provides a soothing anti-allergic cosmetic comprising the plant extract composition described above.

As a further improvement of the above scheme, the cosmetic comprises the following components in percentage by weight:

preferably, the emulsifier is at least one of PEG-40, glyceryl caprylate and lecithin; preferably, the carbomer is carbomer 940, carbomer 941, carbomer 980 or carbomer U20; preferably, the humectant is at least one of glycerin, butylene glycol and ethylene glycol; preferably, the pH regulator is triethanolamine or EDTA-disodium.

Preferably, the cosmetics are cream emulsion type, membrane powder type, gel type, aerosol type or powder cake type cosmetics.

On the other hand, the preparation method of the soothing and antiallergic cosmetic comprises the following steps of: (1) phase A: swelling carbomers with a small amount of water; (2) phase B: dissolving lalang grass rhizome extract with small amount of ethanol, adding emulsifying agent for dissolving, and volatilizing ethanol; (3) phase C: dissolving radix Ophiopogonis extract, herba Portulacae extract, pH regulator, humectant and antiseptic with water; (4) Mixing and dissolving the phase B and the phase C, stirring and dissolving the mixture in the phase A, adjusting the pH value, and filling.

Preferably, in the step (2), the dissolution temperature of the cogongrass rhizome extract in ethanol is 60-70 ℃; preferably, in step (3), the dissolution temperature is 50-60 ℃.

The relieving and anti-sensitization comprises one or more actions of improving skin irritation, reducing skin inflammation, itching and desquamation, relieving abnormal thickening of epidermis layers and reducing the number of mast cells.

The cogongrass rhizome extract is obtained by ethanol extraction and freeze drying. Animal experiments show that the cogongrass rhizome extract as an active ingredient can reduce the skin inflammation desquamation condition, reduce scratching condition, relieve abnormal thickening condition of the epidermis layer and reduce the number of skin mast cells, and can be widely used in skin care products for relieving and resisting sensitization. Cell experiments show that the cogongrass rhizome can play a role in resisting inflammation and relieving skin irritation by being independently used, and the anti-inflammatory effect can be improved by being combined with the ophiopogon root extract and the purslane extract with similar effects. The combined extract is applied to gel cosmetic products, and the prepared products have transparent appearance, fresh and cool use feeling and good moisturizing effect.

The beneficial effects are that: compared with the prior art, the invention has the following remarkable advantages: the ophiopogon root extract has good moisturizing effect, the purslane extract has the effects of resisting oxidization and allergy, and the anti-inflammatory effect can be remarkably improved after the purslane extract is combined with the cogongrass rhizome extract.

Drawings

FIG. 1 is a graph showing the dorsal inflammatory conditions of mice in each group.

FIG. 2 shows the result of hematoxylin-eosin staining of the back skin of mice (200X);

FIG. 3 shows the result of blue staining of toluidine on the back skin of mice (400X);

FIG. 4 shows hyaluronidase inhibitory activity of cogongrass rhizome extract;

FIG. 5 is a graph showing the NO production rate of 100mg/ml extract and the same concentration composition.

Detailed Description

The technical scheme of the invention is further described below with reference to the accompanying drawings.

Embodiment one: preparation of lalang grass rhizome extract

(1) Drying and crushing cogongrass rhizome, and sieving to obtain 60-mesh powder; adding 95% (v/v) ethanol solution with volume of 20 times, mixing, and reflux extracting at 80deg.C under stirring for 2 hr to obtain extractive solution.

(2) Repeating step (1) for three times, mixing the obtained extracts, filtering, collecting filtrate, concentrating the filtrate under reduced pressure at 40deg.C, freeze drying, grinding and pulverizing the dried extract, and sealing and storing.

Embodiment two: preparation of lalang grass rhizome extract

(1) Drying and crushing cogongrass rhizome, and sieving to obtain 80-mesh powder; adding 95% (v/v) ethanol solution with volume of 20 times, mixing, and reflux extracting at 80deg.C under stirring for 2 hr to obtain extractive solution.

(2) Repeating step (1) twice, mixing the obtained extracts, filtering, collecting filtrate, concentrating the filtrate under reduced pressure at 50deg.C, freeze drying, grinding and pulverizing the dried extract, and sealing and storing.

Embodiment III: preparation of lalang grass rhizome extract

(1) Drying and crushing cogongrass rhizome, and sieving to obtain 60-mesh powder; adding 10 times of 95% (v/v) ethanol solution, mixing, and reflux extracting at 70deg.C under stirring for 3 hr to obtain extractive solution.

Embodiment four: preparation of lalang grass rhizome extract

(1) Drying and crushing cogongrass rhizome, and sieving to obtain 80-mesh powder; adding 10 times of 95% (v/v) ethanol solution, mixing, and reflux extracting at 80deg.C under stirring for 2 hr to obtain extractive solution.

Example five

Based on a mouse skin allergy experiment, the relief efficacy of the cogongrass rhizome extract is verified

(1) Grouping and modeling of animals

15 SPF-grade female ICR mice were housed adaptively for one week. On day 0, long hairs of the skin of the back of the mice were removed using an animal hair remover, and were dehaired using a dehairing paste. Mice were randomly grouped according to body weight into 3 groups, which were a blank control group, a model group, and a lalang grass rhizome extract administration group, each group being 5.

100 μl of 1% (w/v) DNCB solution was applied to the back of the mice in the model group and the mice in the dosing group were sensitized on the hair removal sites from day 1, and the blank group was not treated with any treatment, and sensitized 3 times per day.

From

day

8, 100 μl of 0.5% (w/v) DNCB solution was used to apply the excitation to the back dehairing sites of mice in the model group, the intervention group, once every two days.

(2) Preparation and administration method of medicine

Preparing a cogongrass rhizome extract solution:

150mg of the cogongrass rhizome extract powder prepared in the example 1 is precisely weighed, 5mL of physiological saline is added, and the mixture is dissolved by ultrasonic waves to fix the volume.

The administration method comprises the following steps:

from

day

10, 100 μl of cogongrass rhizome extract solution with a concentration of 30mg/ml was smeared on the back modeling site of the mice of the administration group, the mice of the model group were smeared with physiological saline, and the blank control group was not treated with any treatment once a day for 14 consecutive days.

(1) Evaluation of the score of skin inflammation and the number of scratches on the back of the mice: the skin damage condition of the back of the mouse is observed, photographing is carried out regularly, and the specific condition is recorded. The back skin of the mice was scored for four conditions (0 none, 1 mild, 2 moderate, 3 severe). Placing the mice in a conventional mouse cage, adapting to the mice for 30min, observing the back-to-back occlusion of the mouth of the mice, scratching the back by the hind paws, and recording the number of times of scratching the stimulation part of the mice within 15 min.

TABLE 1 Effect of lalang grass rhizome extract on Back inflammation Condition and itching in mice

From the data in Table 1 and as shown in FIG. 1, it was found that the back inflammation level of the mice to which the lalang grass rhizome extract was administered was significantly reduced, and the conditions such as crusting, desquamation, and redness and swelling were reduced, as compared with the mice in the model group. According to the data in Table 1, the number of scratches of the mice in the model group and the mice in the cogongrass rhizome extract administration group was increased, but the number of scratches of the mice in the cogongrass rhizome extract administration group was lower than that in the model group, and the increase amplitude was also lower than that of the mice in the model group. The cogongrass rhizome extract has the functions of reducing inflammation, reducing itching and relieving skin sensitivity.

(2) Mouse back skin epidermis layer thickness measurement

After the last administration, the mice were sacrificed by cervical spine removal, the skin at the back of the mice was peeled off and fixed with 4% (w/v) paraformaldehyde. And (3) carrying out dewatering wax leaching on the fixed skin sample, embedding the skin sample in an embedding machine, and trimming wax blocks. And (3) placing the trimmed wax block into a freezing table at the temperature of minus 20 ℃ for cooling, and then placing the cooled wax block into a paraffin slicer to prepare slices with the thickness of 4 mu m. Paraffin sections were dewaxed to water, stained with hematoxylin, eosin stained, de-watered, and observed under a microscope at 200x magnification, three field-of-view photographic recordings were selected for each section, and the skin layer thickness was determined using imagej software.

TABLE 2 Effect of lalang grass rhizome extract on abnormal proliferation of the epidermal layers of the back skin of mice

From the data shown in FIG. 2 and Table 2, the skin structure of mice in the blank group was regular, the cell morphology was normal, and the tissue structure was normal. The thickness of the epidermis layer of the mice in the model group is obviously increased, a certain degree of hyperkeratosis is caused, and acanthosis exists. Compared with the mice in the model group, the excessive keratinization phenomenon of the epidermis of the mice in the group of the cogongrass rhizome extract administration group is reduced, the thickness of the epidermis layer is obviously lower, and the difference is obvious. The cogongrass rhizome extract has the good effects of improving the skin irritation condition and slowing down the abnormal increase of the epidermal layer thickness.

(3) Mouse back skin mast cell count

Dewaxing the prepared paraffin sections to water, carrying out toluidine blue dyeing, washing with tap water, drying, sealing the sections in a transparent way, magnifying the sections by 400 times under a microscope, selecting three visual fields for each section for observation, and counting the number of mast cells.

TABLE 3 Effect of lalang grass rhizome extract on mice Back skin mast cell infiltration

From the data shown in fig. 3 and table 3, the number of mast cells on the back skin of the mice in the model group was significantly increased compared to that of the mice in the blank group, and the number of mast cells on the back skin of the mice in the lalang grass rhizome extract administration group was significantly different compared to that of the mice in the model group. The mast cells in the skin can generate quick immune response to stimulus factors such as external allergens, and the mast cells can release substances such as cytokines when stimulated, so that the reactions such as inflammation, itching and the like are induced. The cogongrass rhizome extract can reduce the number of mast cells, stabilize the state of the mast cells, relieve the external stimulus to the skin and relieve the skin sensitivity.

Example six

Based on in vitro hyaluronidase activity inhibition test, the anti-allergy relieving effect of cogongrass rhizome extract is verified

0.1ml of 0.25mmol/L CaCl was taken ₂ Incubating the solution with 0.5ml hyaluronidase solution at 37deg.C for 20min; adding 0.5ml of sample solution, and keeping the temperature at 37 ℃ for 20min; adding 0.5ml sodium hyaluronate solution, maintaining at 37deg.C for 30min, and standing at normal temperature for 5min; adding 0.1ml of 0.4mol/L NaOH solution and 0.5ml of acetylacetone solution, placing in a boiling water bath, heating for 15min, and immediately cooling with ice water for 5min; 1.0ml of the Escherichia reagent was added and diluted with 3.0ml of absolute ethanol, followed by standing for 20 minutes to develop a color, and the absorbance was measured by a spectrophotometer. The positive control used dipotassium glycyrrhizinate.

Hyaluronidase is a specific hydrolase of hyaluronic acid, and researches show that the hyaluronidase is closely related to inflammation and anaphylactic reaction of organisms, so that a hyaluronidase activity inhibition test is an important biochemical evaluation method of anti-allergy and relieving activities of substances. The experimental results are shown in FIG. 4. Based on the results of the hyaluronidase activity inhibition test, the inhibition activities of the cogongrass rhizome extract and the positive control dipotassium glycyrrhizinate on the hyaluronidase are enhanced along with the increase of the concentration, and when the concentration of the cogongrass rhizome extract reaches 10mg/ml, the inhibition rate of the cogongrass rhizome extract on the hyaluronidase reaches 84.17 +/-10.32%, which indicates that the cogongrass rhizome extract has good antiallergic and soothing activity.

Example seven

Based on RAW264.7 cell inflammation model, anti-inflammatory efficacy of the composition is verified

Inoculating cells in logarithmic growth phase into 96-well plate with density of 5×10 ⁵ mu.L per well per ml. After cell attachment, RAW264.7 cells were stimulated by the addition of 100ng/ml LPS and 100mg/ml extract, and no extract was added to the model group with 100ng/ml LPS alone. The concentration of the composition is 100mg/ml, and the proportion is cogongrass rhizome extract: radix Ophiopogonis extract: purslane extract 1:1:1. After 24 hours, the cell supernatants were collected and assayed for NO content using gris reagent. The experimental results are shown in fig. 5, and the production rates of inflammatory mediators NO in the model group, the cogongrass rhizome extract group, the dwarf lilyturf tuber extract group, the purslane extract group and the composition group are respectively 99.85+/-0.66%, 78.93+/-1.39%, 75.47 +/-3.00%, 75.90+/-1.09% and 54.40+/-0.25%. The combined extract with the same concentration can obviously improve the anti-inflammatory capability of inhibiting the generation of NO, and is superior to the single application of the extract with the same concentration.

Example eight

The weight percentages are as follows: carbomer 941 type 0.5%, composition 0.5%, EDTA-disodium 0.04%, ethylene glycol 0.4%, butylene glycol 0.4%, glycerin 5%, PEG-40% 3%, p-hydroxyacetophenone 0.4%, the balance being water. Prepared to a 100ml gauge.

According to the above formula, the preparation is carried out according to the following steps:

(1) phase A: swelling carbomer 940 with a small amount of water;

(2) and B phase: dissolving lalang grass rhizome extract with small amount of ethanol at 60-70deg.C, adding PEG-40 for dissolving, and volatilizing ethanol;

(3) and C phase: dissolving radix Ophiopogonis extract, herba Portulacae extract, EDTA-disodium, butanediol, ethylene glycol, glycerol, and p-hydroxyacetophenone with water at 50-60deg.C;

(4) mixing and dissolving the phase B and the phase C, stirring and dissolving the mixture in the phase A, adjusting the pH value, and filling.

Comparative example 1

The formulation used was carbomer 940, otherwise the same as in example 1.

Comparative example 2

Carbomer 980 was used in the formulation, otherwise as in example 1.

Comparative example 3

Carbomer U20 was used in the formulation, otherwise as in example 1.

Comparative example 4

The formulation used was 0.3% carbomer 941, otherwise as in example 1.

Comparative example 5

The formulation used was 0.7% carbomer 941, otherwise as in example 1.

Comparative example 6

The formulation used was 0.9% carbomer 941, otherwise as in example 1.

Comparative example 7

The formulation used was 1.1% carbomer 941, otherwise as in example 1.

Comparative example 8

The composition content in the formulation was 0.3%, otherwise the same as in example 1.

Comparative example 9

The composition content in the formulation was 0.7%, otherwise the same as in example 1.

Comparative example 10

The composition content in the formulation was 0.9%, otherwise the same as in example 1.

Example nine

15 healthy volunteers aged between 20 and 40 years were selected, and after the face was cleaned, example eight and comparative examples 1 to 10 were respectively smeared on both sides of the cheek, and the effect of use was observed and felt.

Scoring was performed according to the following table.

Table 4 shows sensory evaluation criteria of the products

The evaluation experience of volunteers is shown in the following table:

table 5 shows the results of volunteer evaluation

Group of	Evaluation of total score
		Example eight	80.71
Comparative example 1	70.64
		Comparative example 2	67.64
Comparative example 3	75.71
		Comparative example 4	81.00
Comparative example 5	77.00
		Comparative example 6	74.86
Comparative example 7	73.14
		Comparative example 8	79.50
Comparative example 9	82.00
		Comparative example 10	82.21

As is clear from table 5, the evaluation score of example eight was excellent, and the appearance, the feel after application, and the feel after application were all good.

Claims

1. A plant extract composition with anti-allergy and soothing effects, which is characterized by comprising lalang grass rhizome extract, dwarf lilyturf tuber extract and purslane extract.

2. The plant extract composition with antiallergic soothing effect of claim 1, wherein the cogongrass rhizome extract is prepared by the following method: reflux-extracting the dried and pulverized lalang grass rhizome powder with ethanol water solution under stirring, concentrating the filtrate under reduced pressure, and drying.

3. The plant extract composition with antiallergic and soothing effects according to claim 2, wherein in the preparation method of the cogongrass rhizome extract, cogongrass rhizome powder is 60-80 meshes, the stirring reflux extraction time is 2-3 hours, the volume of the ethanol aqueous solution of the extract is 10-20 times of the mass of cogongrass rhizome powder, the extraction times are more than or equal to 2 times, and the temperature of reduced pressure concentration is 40-50 ℃.

4. A soothing anti-sensitization cosmetic, characterized in that it comprises the plant extract composition according to any one of claims 1 to 3.

5. The soothing and anti-sensitization cosmetic according to claim 4, wherein the cosmetic comprises the following components in weight percent:

6. the soothing and anti-sensitization cosmetic according to claim 5, wherein the emulsifier is at least one of PEG-40, caprylic capric glyceride and lecithin; the carbomer is carbomer 940 type, carbomer 941 type, carbomer 980 type or carbomer U20 type.

7. The soothing and anti-sensitization cosmetic according to claim 5, wherein the humectant is at least one of glycerin, butylene glycol and ethylene glycol; the PH regulator is triethanolamine or EDTA-disodium.

8. The soothing and anti-sensitization cosmetic according to claim 7, wherein the cosmetic is a cream emulsion, a film powder, a gel, an aerosol or a powder cake cosmetic.

9. A method for preparing a soothing anti-allergic cosmetic according to any one of claims 5 to 8, characterized in that it comprises the following steps: (1) phase A: swelling carbomers with a small amount of water; (2) phase B: dissolving lalang grass rhizome extract with small amount of ethanol, adding emulsifying agent for dissolving, and volatilizing ethanol; (3) phase C: dissolving radix Ophiopogonis extract, herba Portulacae extract, pH regulator, humectant and antiseptic with water; (4) Mixing and dissolving the phase B and the phase C, stirring and dissolving the mixture in the phase A, adjusting the pH value, and filling.

10. The preparation method according to claim 9, wherein in the step (2), the dissolution temperature of the cogongrass rhizome extract in ethanol is 60-70 ℃; in the step (3), the dissolution temperature is 50-60 ℃.