CN115867653A - 用于检测靶核酸的环状引物和loop-de-loop方法 - Google Patents

用于检测靶核酸的环状引物和loop-de-loop方法 Download PDF

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Publication number
CN115867653A
CN115867653A CN202180021600.4A CN202180021600A CN115867653A CN 115867653 A CN115867653 A CN 115867653A CN 202180021600 A CN202180021600 A CN 202180021600A CN 115867653 A CN115867653 A CN 115867653A
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oligonucleotide
primer
loop
seq
clamp
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卡梅伦·斯科特·博尔
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Aiyou Laboratory Co
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Aiyou Laboratory Co
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels

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  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180021600.4A 2020-03-13 2021-03-12 用于检测靶核酸的环状引物和loop-de-loop方法 Pending CN115867653A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062989140P 2020-03-13 2020-03-13
US62/989,140 2020-03-13
PCT/US2021/022192 WO2021183941A1 (en) 2020-03-13 2021-03-12 Looped primer and loop-de-loop method for detecting target nucleic acid

Publications (1)

Publication Number Publication Date
CN115867653A true CN115867653A (zh) 2023-03-28

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CN202180021600.4A Pending CN115867653A (zh) 2020-03-13 2021-03-12 用于检测靶核酸的环状引物和loop-de-loop方法

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US (3) US11377683B2 (https=)
EP (1) EP4118205A4 (https=)
JP (2) JP7634551B2 (https=)
KR (2) KR102814480B1 (https=)
CN (1) CN115867653A (https=)
AU (1) AU2021236383A1 (https=)
CA (1) CA3174493A1 (https=)
MX (1) MX2022011362A (https=)
WO (1) WO2021183941A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022018741A1 (en) * 2020-07-23 2022-01-27 Indian Institute Of Technology, Kharagpur A point of care (poc) device for nucleic acid based testing and method thereof
US20240376528A1 (en) * 2021-09-13 2024-11-14 Uh-Oh Labs Inc. Looped Primer with Various Internal Modifications and Loop-De-Loop Method for Target Detection
WO2023106866A1 (ko) * 2021-12-10 2023-06-15 주식회사 씨젠 단일 가닥 상호작용적 이중 표지 시스템을 포함하는 태그된 루프프라이머 및 이를 이용한 핵산 등온 증폭 방법
CN118207310A (zh) * 2022-12-08 2024-06-18 广东润鹏生物技术有限公司 一种可剪切环状引物及其试剂盒与扩增方法
GB202408733D0 (en) * 2024-06-18 2024-07-31 Univ Oxford Innovation Ltd Rapid nucleic acid amplification method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US20030148303A1 (en) * 2000-09-19 2003-08-07 Nadeau James G. Universal probes and methods for detection of nucleic acids
US20120164691A1 (en) * 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular dna
CN104694662A (zh) * 2015-04-03 2015-06-10 杜文红 核酸等温扩增反应检测方法及应用该方法的检测试剂盒
CN108431235A (zh) * 2015-12-22 2018-08-21 索灵股份公司 靶核酸的等温环介导扩增(lamp)的荧光检测的方法，寡核苷酸及其试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
ES2327712T3 (es) 2000-09-19 2009-11-03 Eiken Kagaku Kabushiki Kaisha Procedimiento para sintetizar polinucleotidos.
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US10876160B2 (en) 2011-10-31 2020-12-29 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid
US20160289752A1 (en) 2015-03-31 2016-10-06 Transponics Method for the Isothermic Amplification of Nucleic Acid and SNP Detection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US20030148303A1 (en) * 2000-09-19 2003-08-07 Nadeau James G. Universal probes and methods for detection of nucleic acids
US20120164691A1 (en) * 2010-12-27 2012-06-28 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular dna
CN104694662A (zh) * 2015-04-03 2015-06-10 杜文红 核酸等温扩增反应检测方法及应用该方法的检测试剂盒
CN108431235A (zh) * 2015-12-22 2018-08-21 索灵股份公司 靶核酸的等温环介导扩增(lamp)的荧光检测的方法，寡核苷酸及其试剂盒

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Publication number Publication date
JP2023518217A (ja) 2023-04-28
KR20250090353A (ko) 2025-06-19
JP2025072582A (ja) 2025-05-09
US12559793B2 (en) 2026-02-24
BR112022018015A2 (pt) 2022-12-06
US20210285039A1 (en) 2021-09-16
US11753679B2 (en) 2023-09-12
MX2022011362A (es) 2023-02-22
KR102814480B1 (ko) 2025-05-29
EP4118205A4 (en) 2024-04-17
JP7634551B2 (ja) 2025-02-21
AU2021236383A1 (en) 2022-09-22
CA3174493A1 (en) 2021-09-16
KR20230012467A (ko) 2023-01-26
EP4118205A1 (en) 2023-01-18
US11377683B2 (en) 2022-07-05
US20240011083A1 (en) 2024-01-11
WO2021183941A1 (en) 2021-09-16
US20220170086A1 (en) 2022-06-02

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