JP7634551B2 - 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法 - Google Patents

標的核酸を検出するためのループプライマー及びループ・デ・ループ方法 Download PDF

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JP7634551B2
JP7634551B2 JP2022555105A JP2022555105A JP7634551B2 JP 7634551 B2 JP7634551 B2 JP 7634551B2 JP 2022555105 A JP2022555105 A JP 2022555105A JP 2022555105 A JP2022555105 A JP 2022555105A JP 7634551 B2 JP7634551 B2 JP 7634551B2
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primer
loop
oligonucleotide
clamping
sensor molecule
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ボール，キャメロン，スコット
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ユーエイチ－オーエイチ ラブス インコーポレイテッド
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Physics & Mathematics (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022555105A 2020-03-13 2021-03-12 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法 Active JP7634551B2 (ja)

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JP2025019815A JP2025072582A (ja) 2020-03-13 2025-02-10 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法

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US202062989140P 2020-03-13 2020-03-13
US62/989,140 2020-03-13
PCT/US2021/022192 WO2021183941A1 (en) 2020-03-13 2021-03-12 Looped primer and loop-de-loop method for detecting target nucleic acid

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JP2025019815A Pending JP2025072582A (ja) 2020-03-13 2025-02-10 標的核酸を検出するためのループプライマー及びループ・デ・ループ方法

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US (3) US11377683B2 (https=)
EP (1) EP4118205A4 (https=)
JP (2) JP7634551B2 (https=)
KR (2) KR102814480B1 (https=)
CN (1) CN115867653A (https=)
AU (1) AU2021236383A1 (https=)
CA (1) CA3174493A1 (https=)
MX (1) MX2022011362A (https=)
WO (1) WO2021183941A1 (https=)

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Publication number Priority date Publication date Assignee Title
WO2022018741A1 (en) * 2020-07-23 2022-01-27 Indian Institute Of Technology, Kharagpur A point of care (poc) device for nucleic acid based testing and method thereof
US20240376528A1 (en) * 2021-09-13 2024-11-14 Uh-Oh Labs Inc. Looped Primer with Various Internal Modifications and Loop-De-Loop Method for Target Detection
WO2023106866A1 (ko) * 2021-12-10 2023-06-15 주식회사 씨젠 단일 가닥 상호작용적 이중 표지 시스템을 포함하는 태그된 루프프라이머 및 이를 이용한 핵산 등온 증폭 방법
CN118207310A (zh) * 2022-12-08 2024-06-18 广东润鹏生物技术有限公司 一种可剪切环状引物及其试剂盒与扩增方法
GB202408733D0 (en) * 2024-06-18 2024-07-31 Univ Oxford Innovation Ltd Rapid nucleic acid amplification method

Citations (1)

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JP2001513623A (ja) 1996-07-16 2001-09-04 インタージェン カンパニー 分子エネルギー輸送標識を有する核酸増幅オリゴヌクレオチドおよびそれに基づく方法

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US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
US20030148303A1 (en) * 2000-09-19 2003-08-07 Nadeau James G. Universal probes and methods for detection of nucleic acids
ES2327712T3 (es) 2000-09-19 2009-11-03 Eiken Kagaku Kabushiki Kaisha Procedimiento para sintetizar polinucleotidos.
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US9487807B2 (en) * 2010-12-27 2016-11-08 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular DNA
US10876160B2 (en) 2011-10-31 2020-12-29 Eiken Kagaku Kabushiki Kaisha Method for detecting target nucleic acid
US20160289752A1 (en) 2015-03-31 2016-10-06 Transponics Method for the Isothermic Amplification of Nucleic Acid and SNP Detection
CN104694662A (zh) * 2015-04-03 2015-06-10 杜文红 核酸等温扩增反应检测方法及应用该方法的检测试剂盒
ITUB20159332A1 (it) * 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.

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Non-Patent Citations (1)

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THELWELL, Nicola et al.，"Mode of action and application of Scorpion primers to mutation detection"，Nucleic Acids Research，2000年10月01日，Vol. 28，No. 19，pp.3752-3761，DOI: 10.1093/nar/28.19.3752

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JP2023518217A (ja) 2023-04-28
KR20250090353A (ko) 2025-06-19
JP2025072582A (ja) 2025-05-09
US12559793B2 (en) 2026-02-24
BR112022018015A2 (pt) 2022-12-06
US20210285039A1 (en) 2021-09-16
US11753679B2 (en) 2023-09-12
CN115867653A (zh) 2023-03-28
MX2022011362A (es) 2023-02-22
KR102814480B1 (ko) 2025-05-29
EP4118205A4 (en) 2024-04-17
AU2021236383A1 (en) 2022-09-22
CA3174493A1 (en) 2021-09-16
KR20230012467A (ko) 2023-01-26
EP4118205A1 (en) 2023-01-18
US11377683B2 (en) 2022-07-05
US20240011083A1 (en) 2024-01-11
WO2021183941A1 (en) 2021-09-16
US20220170086A1 (en) 2022-06-02

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