CN115836992B - Anti-hair loss shampoo and preparation method thereof - Google Patents
Anti-hair loss shampoo and preparation method thereof Download PDFInfo
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Abstract
The application relates to the technical field of shampoos, and particularly discloses an anti-hair-loss shampoo and a preparation method thereof. The application provides an anticreep shampoo, a serial communication port, including plant extract, plant extract is 1 for weight ratio: (1-2): (0.8-2.5): (0.8-2.5) biota orientalis extract, black sesame extract, arachnoid blue grass extract and savory violet extract; the preparation method of the pansy extract comprises the following steps: carrying out enzymolysis on the herba Violae by adopting enzyme solution to obtain enzymolysis solution; the enzyme in the enzyme liquid is selected from cellulase, hemicellulase, pectinase, snailase, callase and educing enzyme; the application also provides a preparation method of the anti-hair loss shampoo. The anti-hair loss shampoo provided by the application has no stimulation to the body, and has the most remarkable effect of preventing seborrheic alopecia.
Description
Technical Field
The application relates to the technical field of shampoos, in particular to an anti-hair-loss shampoo and a preparation method thereof.
Background
With the acceleration of the life rhythm, the modern young people have more and more serious alopecia problems due to irregular diet, huge life pressure and the like, so that the hair is sparse in the age of many people, even the hair is bald, the light people influence the beauty of the individuals, and the heavy people cause wounds to the psychological activities of the individuals. It was found that hair loss is mainly caused by the following causes: firstly, the ecological scalp of a human body is deteriorated, so that the scalp environment is disturbed, and hair follicles are loosened or blocked; secondly, the metabolism disorder in the body stimulates the sebaceous gland to secrete exuberance, so that a large amount of grease is pressed against the root of the hair, the blood and nutrition supply required by the hair is blocked, and seborrheic alopecia occurs; thirdly, the hair follicle is malnourished and difficult to grow due to the lack of trace elements.
Seborrheic alopecia, also known as androgenetic alopecia, is a major cause of modern hair loss, and its mechanism of hair loss is: human dermal papilla converts testosterone and androstenedione into dihydrotestosterone under the action of 5α -reductase, and dihydrotestosterone binds to androgen receptor on hair follicle cells, resulting in microminiaturization of hair follicle, gradual thinning of hair in anagen phase, and shortened hair growth cycle, which in turn leads to alopecia.
At present, the hair loss prevention shampoo on the market is in full view and uneven in effect level. The traditional anti-hair loss shampoo realizes the anti-hair loss effect by adding chemical substances, however, the shampoo is extremely easy to cause irritation to hair or skin and cause allergy, so that the shampoo is difficult to meet the requirements of the public. In recent years, some anti-hair loss shampoos using plant extracts as main components are appeared, but because the shampoo has short contact time with scalp in the use process, active components in the plant extracts are difficult to fully exert the effects in a short time, so that the anti-hair loss effect is not obvious, and especially the anti-hair loss shampoo has very little effect on preventing seborrheic alopecia.
Therefore, there is an urgent need to develop a shampoo having no irritation to the body and good anti-hair loss effect to meet the needs of the public.
Disclosure of Invention
In order to obtain the shampoo with no stimulation and excellent alopecia preventing function, the application provides the alopecia preventing shampoo and a preparation method thereof.
In a first aspect, the present application provides an anti-hair loss shampoo, which adopts the following technical scheme:
an anti-hair loss shampoo comprises a plant extract, wherein the weight ratio of the plant extract is 1: (1-2): (0.8-2.5): (0.8-2.5) biota orientalis extract, black sesame extract, arachnoid blue grass extract and savory violet extract;
the preparation method of the pansy extract comprises the following steps: carrying out enzymolysis on the herba Violae by adopting enzyme solution to obtain enzymolysis solution; wherein the enzyme in the enzyme solution is selected from the group consisting of cellulase, hemicellulase, pectinase, snailase, callase and educase.
The anti-hair-loss shampoo is prepared by taking the plant extract as the main component, is safe and has no stimulation, and has excellent anti-hair-loss effect, and the anti-hair-loss shampoo not only can solve the problem of hair loss caused by the environmental deterioration of scalp and the necrosis of hair follicle cells, but also can solve the problem of seborrheic hair loss caused by dihydrotestosterone and 5 alpha-reductase on the scalp emulsion, so that the anti-hair-loss shampoo can meet the demands of the masses.
In the application, research shows that the active ingredients in the viola diffusa have good inhibition effect on 5 alpha-reductase, so that the anti-hair loss shampoo is prepared by using the viola diffusa extract, the anti-hair loss shampoo can effectively inhibit the generation of dihydrotestosterone, and the combination of the dihydrotestosterone and androgen receptors on hair follicle cells is reduced, so that the microminiaturization of scalp hair follicles is avoided, and the purpose of preventing alopecia is realized. In addition, the Viola yedoensis extract can accelerate the local blood circulation and metabolism of scalp and promote hair growth. The extract of the arachnoid bluegrass is taken from the root of the arachnoid bluegrass, and ecdysone in the root of the arachnoid bluegrass has good stimulation effect on metabolism of scalp, can promote amino acid to be assembled into protein chains, and can stimulate synthesis of proteins; in addition, the inventor of the application finds that the extract of the arachnoid bluegrass can also improve the hair follicle environment, remove head grease, activate scalp hair follicle cells, promote the differentiation and renewal of the hair follicle cells, and further realize a good hair loss prevention and hair growth effect. The biota orientalis extract is from biota orientalis, and the biota orientalis has the effects of nourishing blood, promoting blood circulation, diminishing inflammation, sterilizing, improving local blood circulation and the like, can promote proliferation of human dermal papilla cells, reduce the level of dihydrotestosterone and the expression of 5 alpha-reductase, and prolong the growing period of hair follicles. The black sesame extract can effectively promote the expression of complex aminopeptidase, promote the synthesis of human melanin, and has good hair blacking effect.
Preferably, the plant extract is in a weight ratio of 1:1.5: (1.3-2): (1.3-2) biota orientalis extract, black sesame extract, arachnoid bluegrass extract and savoury violet herb extract.
In some embodiments, the weight ratio of the biota orientalis extract, the black sesame extract, the arachnoid grass extract, and the savoury violet herb extract may be 1:1.5:1.5: (1.3-2), 1:1.5:1.5: (1.3-1.5), 1:1.5:1.5: (1.5-2), 1:1.5: (1.3-1.5): 1.5, 1:1.5: (1.5-2): 1.5 or 1:1.5: (1.3-2): 1.5.
in a specific embodiment, the weight ratio of the biota orientalis extract, the black sesame extract, the arachnoid grass extract and the savory extract may be 1:1.5:1.5:1.5, 1:1.5:0.8:1.5, 1:1.5:1.3:1.5, 1:1.5:2:1.5, 1:1.5:2.5:1.5, 1:1.5:1.5:0.8, 1:1.5:1.5:1.3, 1:1.5:1.5:2 or 1:1.5:1.5:2.5.
when the content of the pansy extract is too high, firstly, the smell of the shampoo is relatively sharp and can have certain stimulation effect on the scalp, secondly, the sebaceous glands can be caused to be secreted vigorously, and the scalp is easy to oil; when the content of the pansy extract is too low, the active ingredients in the pansy extract are insufficient to realize the inhibition effect on 5 alpha-reductase, so that the anti-falling effect of the shampoo is poor; when the addition amount of the extract of the arachnoid bluegrass is too high, the sebaceous gland secretion is also enhanced. In addition, the application finds that the inhibition effect on 5 alpha-reductase is more remarkable by combining the extract of the arachnoid bluegrass and the extract of the pansy. Therefore, the weight ratio of the arachnoid grass extract to the pansy extract to the other plant extracts is controlled within the range, so that the anti-hair loss shampoo with safety, no stimulation, good anti-hair loss effect and no side effect can be obtained.
Preferably, the enzyme solution has a mass concentration of the enzyme of 0.5-3%.
In a specific embodiment, the enzyme in the enzyme solution may be a cellulase, a pectase, a mixture of a cellulase and a snailase, a mixture of a cellulase and a callose enzyme, a mixture of a pectase and a snailase, or a mixture of a cellulase and a pectase and a snailase.
Preferably, the weight ratio of the enzymes in the enzyme liquid is 1: (0.3-0.6) a mixture of cellulases and helicases.
In a specific embodiment, the weight ratio of cellulase to helicase may also be 1:0.3 or 1:0.6.
the enzyme is used for carrying out enzymolysis on the herba violae, so that the cell wall in the herba violae can be destroyed to the greatest extent, and the active ingredients in the herba violae are fully dissolved. Experiments prove that the cellulase and the snailase in the proportion have better composite enzymolysis effect on the herba coriander, and the obtained herba coriander extract has remarkable effect of preventing alopecia.
Preferably, the preparation method of the pansy extract comprises the following steps:
(1) Pretreating herba Violae, and then carrying out enzymolysis on herba Violae by adopting enzyme solution to obtain enzymolysis solution;
(2) Centrifuging the enzymolysis liquid, and taking supernatant;
(3) Filtering the supernatant with a microporous filter membrane smaller than or equal to 0.5 mu m, and obtaining filtrate;
(4) Then, carrying out chromatography on the filtrate by adopting a chromatographic column, and collecting eluent;
(5) Concentrating the eluent under reduced pressure, and freeze-drying to obtain the herba Violae Hortensis extract.
The application provides a preparation method of a pansy extract, which can effectively extract active ingredients in viola diffusa through enzymolysis, filtration, chromatography and other steps. The research shows that the active ingredient extracted by the preparation method has good inhibition effect on 5 alpha-reductase, so that the shampoo prepared by the active ingredient can reduce the expression of 5 alpha-reductase in scalp, further reduce the conversion of testosterone and androstenedione into dihydrotestosterone, effectively avoid microminiaturization of hair follicles and finally realize the anti-hair loss effect.
Preferably, the chromatographic column is an AB-8 type macroporous resin chromatographic column.
Preferably, the eluting solvent used for the chromatography is water, 50% ethanol, 75% ethanol or 90% ethanol.
Further, the eluting solvent used in the chromatography is 75% ethanol.
The application finds that the obtained pansy extracts by chromatography have a certain inhibition effect on the 5 alpha-reductase by adopting water, 50% ethanol, 75% ethanol or 90% ethanol as eluting solvents respectively, wherein the pansy extracts obtained by eluting with the 75% ethanol have the best inhibition effect on the 5 alpha-reductase.
Preferably, the mass concentration of the plant extract in the anti-hair-loss shampoo is 13-18%.
In the present application, the addition amount of the plant extract is controlled within the above range, so that the purposes of hair growth, hair care and hair loss prevention can be achieved, and the manufacturing cost of the hair loss preventing shampoo can be reduced.
Preferably, the anti-hair loss shampoo further comprises an auxiliary agent; the auxiliary agent is a cleaning agent, a hair conditioner, a humectant, a pearling agent, a flavoring agent, an antistatic agent, a preservative, a thickening agent and a chelating agent.
Preferably, the cleaning agent is sodium lauryl sulfate, cocamidopropyl betaine and sodium laureth sulfate; the hair conditioner is glycerol, polydimethylsiloxane and lanolin; the humectant is betaine; the pearlizing agent is glycol stearate; the aromatic is essence; the antistatic agent is propylene glycol stearate and polyquaternium-10; the preservative is selected from DMDM hydantoin; the thickener is guar hydroxypropyl trimethyl ammonium chloride; the chelating agent is disodium EDTA.
Preferably, the mass concentration of the auxiliary agent in the anti-hair-loss shampoo is 2-3%.
Preferably, the preparation method of the arachnoid bluegrass extract comprises the following steps: taking the roots of the arachnoid bluegrass, cleaning, drying, crushing, adding an enzyme solution with the mass concentration of 0.5-3%, and carrying out enzymolysis for 1-1.5 hours at the temperature of 30-40 ℃; then adding ethanol for soaking for 1-2h, and finally filtering and concentrating to obtain the extract of the arachnoid. Wherein the weight ratio of the enzyme liquid is 1: (0.3-0.6): (0.05-0.15) pectinase, eductase and snailase.
The application provides a preparation method of a spider silk and bluegrass extract, and the method is used for obtaining active ingredients in the spider silk and bluegrass to the greatest extent, and the active ingredients have good anti-drop effect. The shampoo prepared from the active ingredient can effectively improve scalp hair follicle environment, remove head grease, activate scalp hair follicle cell performance, promote hair follicle cell differentiation and update, and realize the effect of preventing alopecia.
In a second aspect, the present application provides a method of preparing an anti-hair loss shampoo.
A preparation method of the anti-hair loss shampoo comprises the following steps:
(1) Dissolving a cleaning agent, a hair conditioner, a humectant, a pearling agent, an antistatic agent, a thickening agent and a chelating agent in water, heating to 75-85 ℃ in a vacuum batching pot, and uniformly stirring to obtain a solution A;
(2) Then cooling the solution A to 45-60 ℃, adding the plant extract, and uniformly stirring to obtain a solution B;
(3) Cooling the solution B to 25-35 ℃, adding the aromatic and the preservative, uniformly stirring, and adjusting the pH value to 6-7 to obtain the anti-hair loss shampoo.
Preferably, the stirring time in the step (2) is less than or equal to 10min.
The application provides a preparation method of an anti-hair loss shampoo, wherein in order to avoid the failure of active ingredients of a plant extract at a medium and high temperature, the stirring time after the plant extract is added cannot exceed 10min.
In summary, the present application has the following beneficial effects:
1. the application provides the anti-hair-loss shampoo which is prepared by using the plant extract and has the advantages of safety, no stimulation and excellent anti-hair-loss effect, and the anti-hair-loss shampoo not only can solve the problem of hair loss caused by the deterioration of scalp environment and the necrosis of hair follicle cells, but also can solve the problem of seborrheic hair loss caused by dihydrotestosterone and 5 alpha-reductase on scalp emulsion, so that the anti-hair-loss shampoo can meet the demands of the masses.
2. In the application, the pansy extract has good inhibition effect on 5 alpha-reductase, so that the shampoo prepared from the pansy extract can reduce the generation of dihydrotestosterone on the scalp, reduce the combination of the dihydrotestosterone and androgen receptors on hair follicle cells, and can avoid microminiaturization of hair follicles of the scalp, thereby achieving the purpose of preventing hair loss; the extract of the arachnoid bluegrass can improve the hair follicle environment, remove head grease, activate scalp hair follicle cells, promote the differentiation and renewal of the hair follicle cells, and further realize good hair loss prevention and hair growth effects.
3. The preparation method sequentially comprises the steps of enzymolysis, filtration, chromatography and the like, and the weight ratio of the preparation method is 1: the cellulase and the snailase (0.3-0.6) are used as enzyme liquid, and the 75% ethanol is used as eluting solvent, so that the active ingredients in the viola diffusa can be extracted to the greatest extent, and experiments show that the extracted active ingredients have good effects of preventing alopecia.
4. The application further controls the weight ratio of the biota orientalis extract, the black sesame extract, the arachnoid bluegrass extract and the pansy extract in the plant extract to be 1:1.5: (1.3-2): in the range of (1.3-2), the prepared hair loss preventing shampoo is safe, has no stimulation, excellent hair loss preventing and hair growing effects, has no side effect, and also has good hair nourishing and blackening effects.
Detailed Description
The application provides an anti-hair-loss shampoo, which comprises 13-18% of a plant extract, 0.8% of a cleaning agent, 0.3% of a hair conditioning agent, 0.4% of a humectant, 0.2% of a pearling agent, 0.2% of a fragrance agent, 0.2% of an antistatic agent, 0.3% of a preservative, 0.2% of a thickening agent, 0.2% of a chelating agent and the balance of water. The weight ratio of the plant extract is 1: (1-2): (0.8-2.5): (0.8-2.5) biota orientalis extract, black sesame extract, arachnoid blue grass extract and savory violet extract; further, the plant extract is prepared from the following components in percentage by weight: 1.5: (1.3-2): (1.3-2) biota orientalis extract, black sesame extract, arachnoid bluegrass and bergamot extract.
In the application, the preparation method of the pansy extract comprises the following steps:
(1) Washing, drying, crushing and sieving with a 30-50 mesh sieve, and then adding enzyme solution with the volume of 10 times and the mass concentration of 0.5-3% for enzymolysis to obtain enzymolysis solution; wherein the enzyme in the enzyme solution is selected from the group consisting of cellulase, hemicellulase, pectinase, helicase, callase and educase; further, the weight ratio of the enzyme in the enzyme liquid is 1: (0.3-0.6) cellulase and helicase.
(2) Centrifuging the enzymolysis solution at 1500-2000r/min for 3-5min, and collecting supernatant;
(3) Filtering the supernatant with microporous membrane smaller than or equal to 0.5 μm, and collecting filtrate;
(4) Then, carrying out chromatography on the filtrate by adopting an AB-8 type macroporous resin chromatographic column, and collecting eluent; wherein the eluting solvent used in chromatography is water, 50% ethanol, 75% ethanol or 90% ethanol;
(5) Concentrating the eluate under reduced pressure at 60-70deg.C, and freeze drying at (-40) - (-50) deg.C to obtain herba Violae Hortensis extract.
In the application, the preparation method of the arachnoid bluegrass extract comprises the following steps: taking the roots of the arachnoid bluegrass, cleaning, drying and crushing, and then adding enzyme solution for enzymolysis for 1-1.5 hours at the temperature of 30-40 ℃; then adding ethanol for soaking for 1-2h, and finally filtering and concentrating to obtain the extract of the arachnoid, wherein the adding amount of the enzyme liquid is 10 times of the volume of the mixture obtained after crushing, and the mass concentration of the enzyme in the enzyme liquid is 0.5-3%.
The preparation method of the anti-hair loss shampoo comprises the following steps:
(1) Dissolving a cleaning agent, a hair conditioner, a humectant, a pearling agent, an antistatic agent, a thickening agent and a chelating agent in water, heating to 75-85 ℃ in a vacuum batching pot, and uniformly stirring to obtain a solution A;
(2) Then cooling the solution A to 45-60 ℃, adding the plant extract, and stirring for 3-10min to obtain a solution B;
(3) Cooling the solution B to 25-35 ℃, adding the aromatic and the preservative, uniformly stirring, and adjusting the pH value to 6-7 to obtain the anti-hair loss shampoo.
In the application, the cleaning agent is sodium lauryl sulfate, cocamidopropyl betaine and sodium laureth sulfate; the hair conditioner is glycerol, polydimethylsiloxane and lanolin; the humectant is betaine; the pearling agent is glycol stearate; the flavoring agent is essence, the antistatic agent is propylene glycol stearate and polyquaternium-10, the preservative is DMDM hydantoin and methylparaben, the thickener is guar gum hydroxypropyl trimethyl ammonium chloride, and the chelating agent is EDTA disodium; the AB-8 type macroporous resin chromatographic column has the specification of 3X 45cm and is purchased from Sanxingzhixin resin Co., ltd; the biota orientalis extract and the black sesame extract are purchased from western safety australian technologies limited company; the remaining materials, reagents, solvents, and the like are commercially available.
The present application is described in further detail below in connection with preparation examples, examples and performance test.
Preparation example
Preparation example 1
Preparation example 1 provides a pansy extract.
The preparation method of the pansy extract comprises the following steps: (1) Washing, drying, crushing and sieving the herba coriander with a 50-mesh sieve, and then adding 2% enzyme solution (prepared by cellulase and snailase in a mass ratio of 1:0.6 and distilled water) for enzymolysis to obtain an enzymolysis solution; wherein the addition amount of the enzyme solution is 10 times of the volume of the product obtained after crushing;
(2) Centrifuging the enzymolysis liquid at 2000r/min for 5min, and collecting supernatant;
(3) Filtering the supernatant with a microporous membrane of 0.5 μm to obtain filtrate;
(4) Then, carrying out chromatography on the filtrate by adopting an AB-8 type macroporous resin chromatographic column, and collecting eluent; wherein, the eluting solvent adopted by the chromatography is water;
(5) Concentrating the eluate at 70deg.C under reduced pressure, and freeze drying at-50deg.C to obtain herba Violae Hortensis extract.
Preparation example 2
Preparation 2 was carried out according to the method provided in preparation 1, except that: the eluting solvent used in the chromatography in step (4) of preparation 2 was 50% ethanol.
Preparation example 3
Preparation 3 was carried out according to the method provided in preparation 1, except that: the eluting solvent used in the chromatography in step (4) of preparation 3 was 75% ethanol.
Preparation example 4
Preparation 4 was carried out according to the method provided in preparation 1, except that: the eluting solvent used in the chromatography in step (4) of preparation example 4 was 90% ethanol.
PREPARATION EXAMPLES 5 to 11
Preparation examples 5 to 11 were each carried out according to the method provided in preparation example 3, except that: the enzyme solutions in step (1) of preparation examples 5 to 11 are specifically shown in Table 1.
TABLE 1 Components and proportions of enzyme solutions in preparation examples 5 to 11
Preparation example 12
Preparation 12 provides an extract of the plant Cynanchum otophyllum.
The preparation method of the arachnoid bluegrass extract comprises the following steps: taking the roots of the arachnoid bluegrass, cleaning, drying and crushing, and then adding enzyme solution for enzymolysis for 1.5 hours at 35 ℃; then adding ethanol to soak for 1.5 hours, and finally filtering and concentrating to obtain the arachnoid, wherein the adding amount of enzyme liquid is 10 times of the volume of the mixture obtained after crushing, the mass concentration of enzyme in the enzyme liquid is 0.5%, and the weight ratio of enzyme in coal industry is 1:0.45:0.1, a mixture of pectinase, educase and helicase.
Preparation example 13
Preparation 13 provides a plant extract.
The extract of pansy provided in preparation example 3 and the extract of arachnoid bluegrass provided in preparation example 12 were mixed according to 1:1 to obtain a plant extract of preparation 13.
Comparative preparation example 1
Comparative preparation 1 provides a pansy extract.
The preparation method of the pansy extract comprises the following steps:
(1) Washing, drying, crushing and sieving the herba coriander with a 50-mesh sieve, and then adding 2% enzyme solution (prepared by cellulase and snailase in a mass ratio of 1:0.6 and distilled water) for enzymolysis to obtain an enzymolysis solution; wherein the addition amount of the enzyme solution is 10 times of the volume of the product obtained after crushing;
(2) Centrifuging the enzymolysis liquid at 2000r/min for 5min, and collecting supernatant;
(3) Filtering the supernatant with a microporous filter membrane with the diameter of 1 μm, and obtaining filtrate;
(4) Then, carrying out chromatography on the filtrate by adopting an AB-8 type macroporous resin chromatographic column, and collecting eluent; wherein, the eluting solvent adopted by the chromatography is water;
(5) Concentrating the eluate at 70deg.C under reduced pressure, and freeze drying at-50deg.C to obtain herba Violae Hortensis extract.
Comparative preparation example 2
Comparative preparation 2 provides a pansy extract.
The preparation method of the pansy extract comprises the following steps:
(1) Washing, drying, crushing and sieving the herba coriander with a 50-mesh sieve, and then adding 2% enzyme solution (prepared by cellulase and snailase in a mass ratio of 1:0.6 and distilled water) for enzymolysis to obtain an enzymolysis solution; wherein the addition amount of the enzyme solution is 10 times of the volume of the product obtained after crushing;
(2) Centrifuging the enzymolysis liquid at 2000r/min for 5min, and collecting supernatant;
(3) Filtering the supernatant with a microporous membrane of 0.5 μm to obtain filtrate;
(4) Concentrating the filtrate under reduced pressure at 70deg.C, and freeze drying at-50deg.C to obtain herba Violae Hortensis extract.
Comparative preparation example 3
Comparative preparation 3 provides a pansy extract.
The preparation method of the pansy extract comprises the following steps:
(1) Washing, drying, crushing and sieving the herba coriander with a 50-mesh sieve, and then adding 2% enzyme solution (prepared by cellulase and snailase in a mass ratio of 1:0.6 and distilled water) for enzymolysis to obtain an enzymolysis solution; wherein the addition amount of the enzyme solution is 10 times of the volume of the product obtained after crushing;
(2) Centrifuging the enzymolysis liquid at 3000r/min for 5min, and collecting supernatant;
(3) Filtering the supernatant with a microporous membrane of 0.5 μm to obtain filtrate;
(4) Then, carrying out chromatography on the filtrate by adopting an AB-8 type macroporous resin chromatographic column, and collecting eluent; wherein, the eluting solvent adopted by the chromatography is water;
(5) Concentrating the eluate at 70deg.C under reduced pressure, and freeze drying at-50deg.C to obtain herba Violae Hortensis extract.
Comparative preparation example 4
Comparative preparation 4 provides an extract of the plant arachnoid bluegrass.
The preparation method of the arachnoid bluegrass extract comprises the following steps: pulverizing herba Hedyotidis Diffusae, adding 55% ethanol with a volume of 8 times of that of the pulverized herba Hedyotidis Diffusae, reflux-extracting at 50deg.C for 3 times for 30min each time, mixing filtrates, filtering, and concentrating to obtain herba Hedyotidis Diffusae extract.
5 alpha reductase inhibition assay
The pansy extracts obtained in preparation examples 1 to 11 and comparative preparation examples 1 to 3, the arachnoid grass extract provided in preparation example 12, and the plant extract provided in preparation example 13 (pansy extract and arachnoid grass extract in a mass ratio of 1:1) were subjected to a 5α -reductase inhibition test.
1. The test method comprises the following steps: preparing a control group solution and a detection group solution respectively, and incubating;
control group solution: the control group solution was an enzyme reaction system, which was incubated at 37℃for 30min.
Detection group solution: dissolving the extract in 50% ethanol to obtain 1% extract solution; 0.5mL of the extract solution was placed in an enzyme reaction system and incubated at 37℃for 30min.
The formulation of the enzyme reaction system is as follows: deionized water 5mL, 1mmol/L testosterone 2. Mu.L, 10mg/mL rat steroid 5. Alpha. Reductase solution 600. Mu.L, 1mmol/L NADPH solution 200. Mu.L
2. The detection method comprises the following steps: detecting absorbance decrease values before and after incubation of the control group solution and the detection group solution under 340nm wavelength respectively by using an ultraviolet-visible spectrophotometer, wherein the absorbance decrease value of the control group solution is recorded as delta A 0 The absorbance decrease values of the solutions in the detection group are sequentially recorded as delta A n （△A 1 ~△A 16 ) And the enzyme activity inhibition rate was calculated.
Enzyme activity inhibition (%) = (Δa) 0 -△A n ）/△A 0 ×100%
Wherein DeltaA 0 As a controlAbsorbance decrease value, Δa, of group solution n Is delta A 1 ~△A 16 The absorbance decrease values of the test group solutions prepared from the extracts of preparation examples 1 to 13 and comparative preparation examples 1 to 3 were shown in this order.
Note that: the 5α reductase is capable of converting testosterone into dihydrotestosterone under the action of NADPH, which upon reaction becomes oxidized nadp+ with characteristic absorption at 340nm, and oxidized nadp+ with no characteristic absorption at 340 nm. Therefore, by comparing the absorbance change values of the control group solution and the detection group solution, the inhibition of the activity of 5α reductase can be judged.
3. Test results: the test results are shown in Table 2.
TABLE 25 alpha-reductase inhibition assay results for Viola sachalinensis extract
From the above results of the test in Table 2, it is understood that the obtained Viola sachalinensis extract of preparation examples 1-11 has remarkable inhibitory effect on 5α -reductase; the extract of the arachnoid bluegrass provided in preparation example 12 has substantially no inhibitory effect on 5α -reductase; while preparation example 13 the plant extract obtained by mixing the pansy extract with the arachnoid bluegrass extract has more remarkable inhibition effect on 5 alpha-reductase; further comparing the results of the preparation examples 1 to 11, it was found that the preparation method of preparation example 3 obtained a more excellent inhibitory effect on 5α -reductase, indicating that in the preparation method of a pansy extract, a mass ratio of 1: the cellulase and the snailase of 0.6 are subjected to enzymolysis, a microporous filter membrane of 0.5 mu m is subjected to filtration, and 75% ethanol is subjected to chromatography, so that active ingredients capable of inhibiting 5 alpha-reductase in the viola diffusa can be obtained to the greatest extent, and the obtained viola extract can be used in shampoo to improve the problem of seborrheic alopecia.
Examples
Example 1
Example 1 provides an anti-hair loss shampoo. The extract of the pansy in example 1 was derived from preparation example 3, and the extract of the arachnoid bluegrass was derived from preparation example 12.
The preparation method of the anti-hair loss shampoo comprises the following steps:
(1) Dissolving a cleaning agent, a hair conditioner, a humectant, a pearling agent, an antistatic agent, a thickening agent and a chelating agent in water, heating to 80 ℃ in a vacuum batching pot, and uniformly stirring to obtain a solution A; the amounts of the components are shown in Table 3;
(2) Then cooling the solution A to 55 ℃, adding 16.5g of plant extract, and uniformly stirring to obtain solution B; wherein, the weight ratio of the plant extracts is 1:1.5:1.5:1.5 biota orientalis leaf extract, black sesame extract, arachnoid blue grass extract and Viola yedoensis extract.
(3) Cooling the solution B to 30 ℃, adding the aromatic and the preservative, uniformly stirring, and adjusting the pH value to 6.5 to obtain the anti-hair loss shampoo.
TABLE 3 amounts of the components of the anti-hair loss shampoo provided in EXAMPLE 1
Examples 2 to 9
Examples 2-9 were each performed according to the procedure of example 1. The difference is that: the weight ratio of each component in the plant extract is shown in table 4.
TABLE 4 weight ratio of the plant extracts of examples 2-9
Comparative example
Comparative example 1
Comparative example 1 was conducted in accordance with the method of example 1 except that: the extract of the Viola sachalinensis in comparative example 1 was derived from comparative preparation example 1, and the extract of the Cyanopsis pinicola was derived from preparation example 12.
Comparative example 2
Comparative example 2 was conducted in accordance with the method of example 1 except that: the extracts of the pansy in comparative example 2 were derived from preparation example 3, and the extract of the spider silk bluish grass was derived from comparative preparation example 4.
Comparative example 3
Comparative example 3 was conducted in accordance with the method of example 1 except that: the plant extract in comparative example 3 was 1 by weight: 1.5:1.5 biota orientalis leaf extract, black sesame extract and arachnoid bluegrass extract.
Comparative example 4
Comparative example 4 was conducted in accordance with the method of example 1 except that: the plant extract in comparative example 4 was 1 by weight: 1.5:1.5 biota orientalis leaf extract, black sesame extract and savoury violet extract.
Comparative example 5
Comparative example 5 was conducted as in example 1 except that: in the preparation method of the anti-hair loss shampoo of the comparative example 5, the stirring time in the step (2) is 20min.
Performance test
Monitoring of hygienic chemical, microbiological, organoleptic and physicochemical indicators
The hygienic chemical, microbiological, sensory and physicochemical indexes of the anti-hair-loss shampoo provided in the example 1 are respectively detected and compared with the QBT 1974-2004 shampoo (cream) industry standard; the anti-hair loss shampoo was subjected to an acute skin irritation test and an acute eye irritation test, and the results are shown in table 5.
Table 5 various index detection results of the anti-hair loss shampoo provided in example 1
Test of anti-drop effect
The anti-hair loss effect of the anti-hair loss shampoos provided in examples 1 to 9 and comparative examples 1 to 5 of the present application was tested, and specifically as follows:
1. test object: 75 hair loss persons have healthy head skin without allergy or scars, and no anti-hair loss product is used within 1 month; 75 baldness sufferers were randomly divided into 15 groups of 5 persons each.
2. The test method comprises the following steps: the anti-hair loss shampoo provided in examples 1-9 and comparative examples 1-5 was used for 14 groups of hair loss patients 1 time every two days, and each time was rubbed for at least 5min; the hair loss inhibitor group 1 uses commercially available hair loss preventing shampoo (mainly comprising folium Platycladi extract, polygoni Multiflori radix extract, and rhizoma Zingiberis recens extract). The hairs are combed 3 times a day in the morning, in the middle and at night, the hairs falling off on the same day are collected, the number of the hairs is recorded, and the average value of each group of alopecia patients is calculated and then the whole number of the hairs is taken.
3. Test results: after 60 days of testing, the statistics are shown in table 6 below.
Table 6 statistical results of anti-drop effect test
According to the detection results of Table 6, the anti-hair loss shampoos provided in examples 1-9 all have anti-hair loss effects, and the anti-hair loss efficiency is more than 60% after 60 days of anti-hair loss test; the anti-hair loss efficiency of the anti-hair loss shampoo provided in comparative examples 1-5 is less than 55%; the anti-falling efficiency of the commercial shampoo is less than 60 percent. Therefore, the anti-hair loss shampoo provided by the application can obviously improve the scalp hair follicle environment of a person suffering from hair loss, and realizes an excellent anti-hair loss effect.
From the detection results of examples 1 to 9, the anti-hair loss efficiency of the anti-hair loss shampoo provided in examples 1, 3 to 4 and 7 to 8 is more than 70%, which means that the weight ratio of the biota orientalis extract, the black sesame extract, the arachnoid blue grass extract and the pansy extract is further controlled to be 1:1.5: (1.3-2): and (1.3-2), the obtained anti-hair loss shampoo has more excellent anti-hair loss effect.
In conclusion, the anti-hair loss shampoo provided by the application is safe, has no stimulation and excellent anti-hair loss function, and can meet the demands of the masses.
While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.
Claims (7)
1. The anti-hair loss shampoo is characterized by comprising a plant extract, wherein the weight ratio of the plant extract is 1: (1-2): (0.8-2.5): (0.8-2.5) biota orientalis extract, black sesame extract, arachnoid blue grass extract and savory violet extract;
the preparation method of the pansy extract comprises the following steps:
(1) Pretreating herba Violae, and then carrying out enzymolysis on herba Violae by adopting enzyme solution to obtain enzymolysis solution; the enzyme in the enzyme liquid is selected from cellulase, hemicellulase, pectinase, snailase, callase and educase;
(2) Centrifuging the enzymolysis liquid, and taking supernatant;
(3) Filtering the supernatant with a microporous filter membrane smaller than or equal to 0.5 mu m, and obtaining filtrate;
(4) Then, carrying out chromatography on the filtrate by adopting a chromatographic column, and collecting eluent; the chromatographic column is an AB-8 type macroporous resin chromatographic column; the eluting solvent adopted by the chromatography is water, 50% ethanol, 75% ethanol or 90% ethanol;
(5) Concentrating the eluent under reduced pressure, and freeze-drying to obtain the herba Violae Hortensis extract.
2. The anti-hair loss shampoo according to claim 1, wherein the plant extract is 1:1.5: (1.3-2): (1.3-2) biota orientalis extract, black sesame extract, arachnoid bluegrass extract and savoury violet herb extract.
3. The anti-hair loss shampoo according to claim 1, wherein the weight ratio of enzymes in the enzyme liquid is 1: (0.3-0.6) a mixture of cellulases and helicases.
4. The anti-hair loss shampoo of any of claims 1-3, further comprising an auxiliary agent; the auxiliary agent is a cleaning agent, a hair conditioner, a humectant, a pearling agent, a flavoring agent, an antistatic agent, a preservative, a thickening agent and a chelating agent.
5. The anti-hair-loss shampoo according to claim 4, wherein the mass concentration of the auxiliary agent in the anti-hair-loss shampoo is 2-3%.
6. The method for preparing the anti-hair loss shampoo according to claim 4 or 5, comprising the following steps:
(1) Dissolving a cleaning agent, a hair conditioner, a humectant, a pearling agent, an antistatic agent, a thickening agent and a chelating agent in water, heating to 75-85 ℃ in a vacuum batching pot, and uniformly stirring to obtain a solution A;
(2) Then cooling the solution A to 45-60 ℃, adding the plant extract, and uniformly stirring to obtain a solution B;
(3) Cooling the solution B to 25-35 ℃, adding the aromatic and the preservative, uniformly stirring, and adjusting the pH value to 6-7 to obtain the anti-hair loss shampoo.
7. The method of claim 6, wherein the stirring time in the step (2) is less than or equal to 10min.
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