CN115819613B - 基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用 - Google Patents

基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用 Download PDF

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CN115819613B
CN115819613B CN202210507054.1A CN202210507054A CN115819613B CN 115819613 B CN115819613 B CN 115819613B CN 202210507054 A CN202210507054 A CN 202210507054A CN 115819613 B CN115819613 B CN 115819613B
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cells
leu
cell
msln
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CN115819613A (zh
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赵旭东
郭婧
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West China Hospital of Sichuan University
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/28Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00General protective or antinoxious agents
    • A61P39/06Free radical scavengers or antioxidants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
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    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material

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CN202210507054.1A 2022-05-10 2022-05-10 基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用 Active CN115819613B (zh)

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Application Number Priority Date Filing Date Title
CN202210507054.1A CN115819613B (zh) 2022-05-10 2022-05-10 基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用
PCT/CN2023/093318 WO2023217192A1 (fr) 2022-05-10 2023-05-10 Préparation d'une cellule immunitaire de récepteur antigénique chimérique construite sur la base d'une protéine précurseur de msln et son utilisation

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CN202210507054.1A CN115819613B (zh) 2022-05-10 2022-05-10 基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用

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CN115819613B true CN115819613B (zh) 2023-10-20

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Publication number Priority date Publication date Assignee Title
CN115819613B (zh) * 2022-05-10 2023-10-20 四川大学华西医院 基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN110527667A (zh) * 2018-05-25 2019-12-03 深圳宾德生物技术有限公司 一种靶向muc16的嵌合抗原受体和嵌合抗原受体t细胞及其制备方法和应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010065044A1 (fr) * 2008-12-05 2010-06-10 The Government Of The U.S.A., As Represented By The Secretary, Department Of Health & Human Services Fragments peptidiques bloquants de mésothéline
CN106480097A (zh) * 2016-10-13 2017-03-08 南京凯地生物科技有限公司 利用CRISPR/Cas9技术敲除人PD‑1基因构建可靶向MSLN新型CAR‑T细胞的方法及其应用
CN107557336B (zh) * 2017-09-15 2020-02-14 山东兴瑞生物科技有限公司 一种抗muc16安全型嵌合抗原受体修饰的免疫细胞及其应用
CN112533957A (zh) * 2018-06-04 2021-03-19 普瑞赛格恩公司 Muc16特异性嵌合抗原受体及其用途
US20210040174A1 (en) * 2019-08-08 2021-02-11 Navrogen, Inc. Composition and Use of Humoral Immune Suppressor Antagonists for the Treatment of Humoral Immune Suppressed Diseases
WO2021155034A1 (fr) * 2020-01-29 2021-08-05 TCR2 Therapeutics Inc. Compositions et procédés pour la reprogrammation du tcr mettant en oeuvre des protéines de fusion spécifiques muc16
CN111925990B (zh) * 2020-09-02 2022-08-26 北京立康生命科技有限公司 一种针对卵巢癌的Anti-MUC16 CAR-T细胞及其制备方法
CN115819613B (zh) * 2022-05-10 2023-10-20 四川大学华西医院 基于msln前体蛋白构建的嵌合抗原受体免疫细胞制备及其应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110527667A (zh) * 2018-05-25 2019-12-03 深圳宾德生物技术有限公司 一种靶向muc16的嵌合抗原受体和嵌合抗原受体t细胞及其制备方法和应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Ligand-based adoptive T cell targeting CA125 in ovarian cancer;Haihong Zhao et al;Research Square;第1-20页 *

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WO2023217192A1 (fr) 2023-11-16

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