CN115778880A - Liposome, preparation method and application thereof - Google Patents

Liposome, preparation method and application thereof Download PDF

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Publication number
CN115778880A
CN115778880A CN202310048818.XA CN202310048818A CN115778880A CN 115778880 A CN115778880 A CN 115778880A CN 202310048818 A CN202310048818 A CN 202310048818A CN 115778880 A CN115778880 A CN 115778880A
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liposome
whitening
percent
mixture
homogenization
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刘帅
李阳
周建青
才英明
刘艳梅
徐晓平
胡舜
王振
周婷婷
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Carlyle Cosmetics Co ltd
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Carlyle Cosmetics Co ltd
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention provides a liposome and a preparation method and application thereof, belonging to the technical field of daily chemicals. The invention prepares the liposome with the whitening effect which can act on the epidermal layer and the dermal layer by selecting two substances with the optimal whitening effect to carry out organic combination; and the transdermal performance and the slow release performance of the liposome are enhanced by a specific liposome coating technology. The liposome prepared by the invention can be applied to whitening cosmetics, in particular to whitening cream, whitening emulsion and whitening spray.

Description

Liposome, preparation method and application thereof
Technical Field
The invention belongs to the technical field of daily chemicals, and particularly relates to a liposome and a preparation method and application thereof.
Background
The liposome is composed of amphiphilic molecules, the capsule wall of the liposome is composed of bimolecular membranes, the hydrophilic head part of the amphiphilic molecules forms the inner and outer surfaces of the membranes, the lipophilic tail part of the amphiphilic molecules is positioned in the middle of the membranes, and the structure of the liposome can encapsulate water-soluble and fat-soluble substances. The whole structure is similar to the structure of a biological cell membrane, and lipoid such as phospholipid and the like which are main components of the liposome are also main components of the biological cell membrane, so the liposome can be fused with human epidermal cells, easily penetrates through skin cutin to enter epidermis and dermis, and effectively promotes the permeation of the components; in addition, the particle size and good liquid state and deformability are beneficial to the liposome to penetrate the epidermis barrier, so that the encapsulated effective components can enter the dermis, the biochemical reaction related to the membrane can be activated, various functions can be directly and durably exerted inside and outside cells, and various health care and beauty effects such as whitening, moistening, crease resistance, aging resistance, freckle removal, acne prevention, sun protection and the like on the skin are realized.
The natural whitening component pterostilbene is widely applied in the field of cosmetics in recent years. In the aspect of skin, pterostilbene has unique chemical and physiological activities and can play multiple roles in skin care products. As an effective whitening active ingredient, pterostilbene is pure natural, has an ultraviolet absorption effect, has high-efficiency antioxidant activity in a physiological environment, and can be oxidized to release H by a plurality of phenolic hydroxyl groups in a molecular structure + The lipid peroxide inhibitor is competitively combined with free radicals and oxides to protect lipid and block the chain reaction of the free radicals, is a good free radical scavenger and lipid peroxidation inhibitor, has stronger capability of scavenging oxygen free radicals than common natural antioxidants such as resveratrol, vitamin C, arbutin, kojic acid and the like, and can protect the organism from being damaged by the oxygen free radicals at low concentration.
Glabridin extracted from glycyrrhiza glabra root is one of the most effective natural whitening products in whitening, can penetrate into the skin and keep high activity, effectively inhibits the activity of various enzymes in the melanin generation process, has oxygen radical scavenging capacity similar to that of SOD (superoxide dismutase), and has oxygen radical resisting capacity similar to that of vitamin E.
With the increasing consumer consumption level, natural ingredient cosmetics are in favor. With the progress of scientific technology, many novel technologies are applied to cosmetic products to enable effective components to better play roles while modern skin physiology based on molecular biology gradually reveals skin damage and aging mechanisms.
The international natural raw materials are receiving more and more attention, and reports of researching natural active ingredients in plants and wrapping the active ingredients with slow-release carriers for cosmetics are increasing, but most of plant extracts used at present are crude extracts and are not completely pure active substances in the plants, and reports of wrapping two fat-soluble active ingredients simultaneously in the study of wrapping the active ingredients with liposomes are less, and particularly, the study of wrapping pterostilbene and glabridin with hydrogenated lecithin liposomes for whitening cosmetics is not reported yet.
Disclosure of Invention
In view of the above, the invention aims to provide a liposome and a preparation method and application thereof, and the liposome which can act on the epidermis layer and the dermis layer and has the whitening effect is prepared by selecting two substances with optimal whitening effect and organically combining the two substances; and the transdermal performance and the slow release performance of the liposome are enhanced by a specific liposome coating technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the liposome is prepared from the following components in percentage by mass: 1 to 5 percent of hydrogenated lecithin, 1 to 5 percent of caprylic/capric triglyceride, 1 to 3 percent of glycyrrhiza glabra root extract, 1 to 5 percent of pterostilbene, 25 to 85 percent of water, 1 to 50 percent of glycerol, 2 to 5 percent of 1, 2-pentanediol and 2 to 6 percent of 1, 3-butanediol.
The invention also provides a preparation method of the liposome, which comprises the following steps:
(1) Mixing glycerol, 1, 2-pentanediol, 1, 3-butanediol and water, heating to 75-90 ℃, and cooling to 50-70 ℃ to obtain a mixture I;
(2) Mixing caprylic/capric triglyceride, glycyrrhiza glabra root extract and pterostilbene, adding hydrogenated lecithin, and emulsifying under vacuum to obtain a mixture II;
(3) Mixing the mixture I in the step (1) and the mixture II in the step (2), and performing vacuum homogenization treatment to obtain a coarse emulsion dispersion;
(4) And (3) carrying out high-pressure homogenization treatment on the coarse emulsion dispersion, taking out the coarse emulsion dispersion after the coarse emulsion dispersion is homogenized to be clear and transparent, and naturally cooling to obtain the liposome.
Preferably, the vacuum degree of the vacuum condition in the step (2) is-0.03 to-0.07 mpa, and the temperature of the mixture II is 50 to 65 ℃.
Preferably, the vacuum degree of the vacuum in the step (3) is-0.03 to-0.07 mpa, the rotation speed of the homogenizing is 6000 to 30000r/min, the homogenizing time is 5 to 30min, and the homogenizing temperature is 60 to 70 ℃.
Preferably, the high pressure in step (4) is 60-150 mpa, and the homogenization temperature is 60-70 ℃.
The invention also provides application of the liposome in whitening cosmetics.
Preferably, the addition amount of the liposome in the whitening cosmetic is 1-3g.
The invention also provides a whitening cosmetic comprising the liposome.
Preferably, the whitening cosmetic comprises a whitening cream, a whitening emulsion or a whitening spray.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, two substances with good whitening effects (namely pterostilbene and glycyrrhiza glabra extract) are selected to be organically combined, so that the liposome with the whitening effect, which can act on an epidermal layer and a dermal layer, is obtained; by simultaneously encapsulating pterostilbene and glycyrrhiza glabra extracts by using a liposome technology, the transdermal property and the slow release property of the liposome are enhanced, and a better whitening effect is achieved. Compared with other technologies, the whitening cream has the effects of whitening immediately and for a long time, and can fundamentally whiten the skin.
Detailed Description
The invention provides a liposome which is prepared from the following components in percentage by mass: 1 to 5 percent of hydrogenated lecithin, 1 to 5 percent of caprylic/capric triglyceride, 1 to 3 percent of glycyrrhiza glabra root extract, 1 to 5 percent of pterostilbene, 25 to 85 percent of water, 1 to 50 percent of glycerol, 2 to 5 percent of 1, 2-pentanediol and 2 to 6 percent of 1, 3-butanediol.
In the present invention, the liposome comprises hydrogenated lecithin 1 to 5%, preferably 2 to 4%, and more preferably 3%; the hydrogenated lecithin has strong hydrophilicity and moisture retention property, and has strong affinity to skin and mucous membrane.
In the invention, the liposome comprises 1-5%, preferably 2-4%, and more preferably 3% of caprylic/capric triglyceride which can be used as a base material of moisturizing factors and a stabilizing agent, an antifreezing agent and a homogenizing agent of cosmetics to lubricate and luster the skin.
In the present invention, the liposome comprises 1 to 3%, preferably 2% of glycyrrhiza glabra root extract; the Glycyrrhiza glabra root extract can penetrate into the skin, keeps high activity, can effectively inhibit the activity of various enzymes in the melanin generation process, and has the effects of resisting bacteria, diminishing inflammation, whitening and brightening the skin, resisting aging and the like.
In the invention, the liposome comprises 1 to 5 percent of pterostilbene, preferably 2 to 4 percent, and further preferably 3 percent; the pterostilbene has ultraviolet absorption effect, has high-efficiency antioxidant activity in physiological environment, and multiple phenolic hydroxyl groups in a molecular structure can be oxidized to release H + Competitively combines with free radical and oxide, protects lipid, blocks chain reaction of free radical, and is a good free radical scavenger and lipid peroxidation inhibitor.
In the present invention, the liposome comprises 1 to 50% of glycerol, preferably 10 to 40%, more preferably 20 to 30%, and still more preferably 25%; the glycerol has effects of keeping moisture, moistening, resisting oxidation, etc.
In the present invention, the liposome comprises 2 to 5%, preferably 3 to 4%, and more preferably 3.5% of 1, 2-pentanediol; the 1, 2-pentanediol has excellent moisturizing performance and an antiseptic effect.
In the present invention, the liposome comprises 1, 3-butanediol 2-6%, preferably 3-5%, and more preferably 4%; the 1, 3-butanediol has good moisturizing performance.
In the present invention, the liposome comprises water 25-85%, preferably 30-70%, and more preferably 45%; the water is preferably deionized water.
The hydrogenated lecithin, caprylic/capric triglyceride, glycyrrhiza glabra root extract, pterostilbene, glycerol, 1, 2-pentanediol and 1, 3-butanediol are all commercially available products.
The invention also provides a preparation method of the liposome, which comprises the following steps:
(1) Mixing glycerol, 1, 2-pentanediol, 1, 3-butanediol and water, heating to 75-90 ℃, and cooling to 50-70 ℃ to obtain a mixture I;
(2) Mixing caprylic/capric triglyceride, glycyrrhiza glabra root extract and pterostilbene, adding hydrogenated lecithin, and emulsifying under vacuum to obtain a mixture II;
(3) Mixing the mixture I in the step (1) and the mixture II in the step (2), and carrying out vacuum homogenization treatment to obtain a coarse emulsion dispersion;
(4) And (3) carrying out high-pressure homogenization treatment on the coarse emulsion dispersion, taking out the coarse emulsion dispersion after the coarse emulsion dispersion is homogenized to be clear and transparent, and naturally cooling to obtain the liposome.
In the invention, glycerol, 1, 2-pentanediol, 1, 3-butanediol and water are mixed and heated to 75-90 ℃, and then cooled to 50-70 ℃ to prepare a mixture I.
In the present invention, the heating is performed to 75 to 90 ℃, preferably 78 to 85 ℃, and more preferably 80 ℃; the temperature is reduced to 50-70 ℃, preferably to 55-65 ℃, and further preferably to 60 ℃.
While preparing the mixture I, the caprylic/capric triglyceride, the glycyrrhiza glabra root extract and the pterostilbene are mixed, then hydrogenated lecithin is added, and emulsification treatment is carried out under a vacuum condition, so as to prepare a mixture II.
In the present invention, the degree of vacuum under the vacuum condition is preferably-0.03 to-0.07 mpa, more preferably-0.04 to-0.06 mpa, and still more preferably-0.05 mpa; the temperature of the mixture II is preferably 50-65 ℃, more preferably 55-60 ℃, and even more preferably 57 ℃; the emulsification is accompanied by stirring and heating treatment, and the stirring and heating time, temperature, speed and the like are not particularly limited, so that the mixture II is heated to 50-65 ℃, namely, the emulsification treatment is stopped when the temperature of the mixture II reaches 50-65 ℃.
After a mixture I and a mixture II are obtained, the mixture I in the step (1) and the mixture II in the step (2) are mixed and subjected to vacuum homogenization treatment to obtain a coarse milk dispersion.
In the present invention, the degree of vacuum of the vacuum is preferably-0.03 to-0.07 mpa, more preferably-0.04 to-0.06 mpa, and still more preferably-0.05 mpa; the rotation speed of the homogenization is preferably 6000 to 30000r/min, more preferably 10000 to 25000r/min, and still more preferably 15000 to 20000r/min; the homogenizing time is preferably 5-30 min, more preferably 10-25 min, and even more preferably 20min; the temperature for homogenization is preferably 60 to 70 ℃, more preferably 62 to 68 ℃, and still more preferably 65 ℃.
After the coarse emulsion dispersion is obtained, the coarse emulsion dispersion is subjected to high-pressure homogenization treatment, and is taken out after being homogenized to be clear and transparent, and is naturally cooled, so that the liposome is obtained.
In the present invention, the pressure for homogenization is preferably 60 to 150mpa, more preferably 70 to 130mpa, and still more preferably 100mpa; the temperature for homogenization is preferably 60 to 70 ℃, more preferably 62 to 67 ℃, and still more preferably 65 ℃.
After the liposome is obtained, the liposome is bottled and sealed for storage.
The invention also provides application of the liposome in whitening cosmetics.
In the present invention, the liposome is preferably added in an amount of 1 to 3g, more preferably 2g, in the whitening cosmetic.
The invention also provides a whitening cosmetic comprising the liposome.
In the present invention, the whitening cosmetics include whitening cream, whitening lotion or whitening spray.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A liposome comprises the following raw material components: 5g of hydrogenated lecithin; 1g of caprylic/capric triglyceride, 1g of glycyrrhiza glabra root extract and 5g of pterostilbene; 27g of deionized water, 50g of glycerol, 5g of 1, 2-pentanediol, and 6g of 1, 3-butanediol.
The preparation method of the liposome comprises the following steps:
(1) Mixing glycerol, 1, 2-pentanediol, 1, 3-butanediol and water, heating to 75 ℃, and cooling to 50 ℃ to obtain a mixture I;
(2) Mixing caprylic/capric triglyceride, glycyrrhiza glabra root extract and pterostilbene, adding hydrogenated lecithin, setting the vacuum degree to-0.03 mpa, heating while stirring, stopping stirring and heating when the temperature reaches 50 ℃, and preparing a mixture II;
(3) Mixing the mixture I in the step (1) and the mixture II in the step (2), setting the vacuum degree to be-0.07 mpa, the homogenizing temperature to be 60 ℃, the homogenizing rotating speed to be 6000r/min, and homogenizing for 30min to obtain a coarse emulsion dispersion;
(4) Setting the homogenization pressure at 60mpa and the homogenization temperature at 60 ℃, taking out the crude milk dispersion after the crude milk dispersion is homogenized to be clear and transparent, and naturally cooling to obtain the liposome.
Example 2
A liposome comprises the following raw material components: 1g of hydrogenated lecithin; 5g of caprylic/capric triglyceride, 3g of glycyrrhiza glabra root extract and 1g of pterostilbene; 85g of deionized water, 1g of glycerol, 2g of 1, 2-pentanediol, and 2g of 1, 3-butanediol.
The preparation method of the liposome comprises the following steps:
(1) Mixing glycerol, 1, 2-pentanediol, 1, 3-butanediol and water, heating to 90 ℃, and cooling to 70 ℃ to obtain a mixture I;
(2) Mixing caprylic/capric triglyceride, glycyrrhiza glabra root extract and pterostilbene, adding hydrogenated lecithin, setting the vacuum degree to-0.07 mpa, heating while stirring, stopping stirring and heating when the temperature reaches 65 ℃, and preparing a mixture II;
(3) Mixing the mixture I in the step (1) and the mixture II in the step (2), setting the vacuum degree to be-0.03 mpa, the homogenizing temperature to be 70 ℃, the homogenizing rotating speed to be 30000r/min, and homogenizing for 5min to obtain a coarse emulsion dispersion;
(4) Setting the homogenizing pressure at 150mpa and the homogenizing temperature at 70 ℃, taking out the crude emulsion dispersion after the crude emulsion dispersion is homogenized to be clear and transparent, and naturally cooling to obtain the liposome.
Example 3
A liposome comprises the following raw material components: 3% of hydrogenated lecithin; caprylic/capric triglyceride 3%, glycyrrhiza glabra root extract 2%, pterostilbene 3%; 56.5 percent of deionized water, 25 percent of glycerol, 3.5 percent of 1, 2-pentanediol and 4 percent of 1, 3-butanediol.
The preparation method of the liposome comprises the following steps:
(1) Mixing glycerol, 1, 2-pentanediol, 1, 3-butanediol and water, heating to 80 ℃, and cooling to 60 ℃ to obtain a mixture I;
(2) Mixing caprylic/capric triglyceride, glycyrrhiza glabra root extract and pterostilbene, adding hydrogenated lecithin, setting the vacuum degree to-0.05 mpa, heating while stirring, stopping stirring and heating when the temperature reaches 57 ℃, and preparing a mixture II;
(3) Mixing the mixture I in the step (1) and the mixture II in the step (2), setting the vacuum degree to be-0.05 mpa, the homogenizing temperature to be 65 ℃, the homogenizing rotating speed to be 15000r/min, and homogenizing for 20min to obtain a coarse emulsion dispersion;
(4) Setting the homogenizing pressure at 100mpa and the homogenizing temperature at 65 ℃, taking out the crude emulsion dispersion after the crude emulsion dispersion is homogenized to be clear and transparent, and naturally cooling to obtain the liposome.
Example 4
The liposome prepared in example 1 was added to whitening cream in an amount of 3g/100g to prepare whitening cream with whitening effect.
The whitening cream comprises the following raw material components: 3g of liposome, 74.2g of deionized water, 4g of glycerol, 4.5g of 1, 3-butanediol, 0.05g of EDTA-2Na, 0.5g of 1, 2-hexanediol, 0.5g of p-hydroxyacetophenone, 0.30g of carbomer, 0.15g of xanthan gum, 3g of caprylic/capric triglyceride, 2.5g of squalane, 1.5g of cetearyl alcohol, 1g of shea butter, 2g of meadowfoam seed oil, 2.5g of glycerol stearate citrate and 0.30g of arginine.
Example 5
The liposome prepared in example 2 was added to a whitening spray in an amount of 1g/100g to prepare a whitening spray having whitening effects.
The other raw material components in the whitening spray comprise: 1g of liposome, 91.7g of deionized water, 3g of glycerol, 3g of 1, 3-butanediol, 0.5g of 1, 2-hexanediol, 0.5g of p-hydroxyacetophenone, 0.1g of carbomer, 0.1g of xanthan gum and 0.1g of arginine.
Example 6
The liposome prepared in example 3 was added to a whitening emulsion in an amount of 2g/100g to prepare a whitening emulsion having whitening effects.
The whitening emulsion comprises the following other raw material components: 2g of liposome, 80.58g of deionized water, 4g of glycerol, 3g of 1, 3-butanediol, 0.5g of 1, 2-hexanediol, 0.5g of p-hydroxyacetophenone, 0.15g of carbomer, 0.12g of xanthan gum, 3g of caprylic/capric triglyceride, 1g of squalane, 0.5g of cetostearyl alcohol, 1g of shea butter, 1.5g of meadowfoam seed oil, 2g of glycerol stearate citrate and 0.15g of arginine.
Comparative example 1
A whitening cream was prepared from the same raw material components as in example 4, but without the addition of the liposomes prepared in example 1.
Comparative example 2
A whitening spray was prepared in the same manner as the raw material components of example 5, except that the liposome prepared in example 2 was not added.
Comparative example 3
A whitening emulsion was prepared in the same manner as the raw material components of example 6, except that the liposome prepared in example 3 was not added.
Comparative experiment 1
15 subjects were selected and tested for moisturizing effect after 8 hours using the whitening creams prepared in example 4 and comparative example 1.
The specific method comprises the following steps: selecting the curved side of forearm of subject, measuring area of 2 × 2cm, selecting 2 positions on both arms, cleaning, and spreading the product on the area to be measured; the measurement of the stratum corneum moisture content of the area to be tested was carried out using a Corneometer (skin moisture tester) at 0h, 4h and 8h before and after use, respectively. After 5 determinations of the same locus to exclude the maximum and minimum, the average of 3 determinations was recorded and the results are shown in Table 1.
TABLE 1 Effect of different samples on moisture content of stratum corneum
Figure BDA0004056782830000081
Figure BDA0004056782830000091
As can be seen from table 1, the moisturizing effect of example 4 with the liposome of the present invention is significantly increased as compared to the moisturizing effect of the whitening cream without the liposome of the present invention.
Comparative experiment 2
The whitening emulsions prepared in example 6 and comparative example 3 were used for 20 women aged 22 to 49 as subjects, and the whitening effect of the subjects after 3 weeks of use was observed.
The specific method comprises the following steps: after washing the face in the morning and evening every day, an appropriate amount of the preparation was applied to each of 4 parts of the face and forearm of each subject, the two whitening lotions were used simultaneously for a period of 3 weeks, and the whitening effect of the preparation was evaluated using a colorimeter according to the following criteria.
Adopting a double-blind method:
the standard is as follows: +: the skin surface becomes obviously white; + -: slight whitening of the skin surface; -: there was no change.
For each subject, the lightness of the skin of the forearm site before and after the use of each whitening emulsion was measured with a color difference meter, wherein the greater the difference between the lightness values of the skin before and after the use, the brighter the skin. At the same time, the effect on freckles and spots on the face was observed. The results obtained are shown in table 2:
table 2 whitening effect of whitening emulsion
Figure BDA0004056782830000092
As can be seen from table 2, the whitening emulsion containing the liposome of the present invention has a significantly better whitening effect than the whitening emulsion prepared without the liposome of the present invention.
Comparative experiment 3: inhibition of tyrosinase assay
L-tyrosine was placed in a sulfuric acid buffer solution (pH 6.8) containing copper sulfate and tyrosinase solutions, and the liposome prepared in example 3, ascorbic acid derivative, arbutin and hydroquinone were added to each solution, respectively, and incubated at 37 ℃ for 1 hour, and the absorbance of the solution was measured. The measurement wavelength used was 640nm (absorption wavelength of white dopachrome is 640 nm). And calculating the inhibition rate of tyrosinase of different solutions according to the following formula:
inhibition% = (Ab- (A) t -A 0 )/Ab)×100
Ab: absorbance of the solution used
A t : absorbance of solution without using whitening effect liposome
A 0 : absorbance of solution without tyrosinase
The test results are shown in table 3.
TABLE 3 comparison of tyrosinase inhibitory Activity
Measuring substance IC 50 Value (mg) Relative degree of activity
Liposomes 0.0025 91
Ascorbic acid derivatives 0.275 1.0
Arbutin ＞2.0 --
Hydroquinone 0.017 17.0
IC 50 The value: the concentration of inhibitor required to inhibit 50% of tyrosinase activity.
As can be seen from Table 3, IC was obtained 50 The value required for the liposome concentration was 0.0025mg. Therefore, the liposome prepared by the invention can obviously inhibit the activity of tyrosinase.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.

Claims (9)

1. The liposome is characterized by being prepared from the following components in percentage by mass: 1 to 5 percent of hydrogenated lecithin, 1 to 5 percent of caprylic/capric triglyceride, 1 to 3 percent of glycyrrhiza glabra root extract, 1 to 5 percent of pterostilbene, 25 to 85 percent of water, 1 to 50 percent of glycerol, 2 to 5 percent of 1, 2-pentanediol and 2 to 6 percent of 1, 3-butanediol.
2. The method for preparing the liposome of claim 1, comprising the steps of:
(1) Mixing glycerol, 1, 2-pentanediol, 1, 3-butanediol and water, heating to 75-90 ℃, and cooling to 50-70 ℃ to obtain a mixture I;
(2) Mixing caprylic/capric triglyceride, glycyrrhiza glabra root extract and pterostilbene, adding hydrogenated lecithin, and emulsifying under vacuum to obtain a mixture II;
(3) Mixing the mixture I in the step (1) and the mixture II in the step (2), and carrying out vacuum homogenization treatment to obtain a coarse emulsion dispersion;
(4) And (3) carrying out high-pressure homogenization treatment on the coarse emulsion dispersion, taking out the coarse emulsion dispersion after the coarse emulsion dispersion is homogenized to be clear and transparent, and naturally cooling to obtain the liposome.
3. The method for preparing liposome of claim 2, wherein the vacuum condition in step (2) is-0.03 mpa to-0.07 mpa, and the temperature of the mixture II is 50-65 ℃.
4. The method for preparing liposome according to claim 2, wherein the vacuum degree of the vacuum in the step (3) is-0.03 to-0.07 mpa, the rotation speed of the homogenization is 6000 to 30000r/min, the time of the homogenization is 5 to 30min, and the temperature of the homogenization is 60 to 70 ℃.
5. The method for preparing the liposome according to claim 2, wherein the high pressure in the step (4) is 60 to 150mpa, and the homogenization temperature is 60 to 70 ℃.
6. Use of the liposome of claim 1 or the liposome prepared by the preparation method of any one of claims 2 to 5 in whitening cosmetics.
7. A whitening cosmetic comprising the liposome according to claim 1 or the liposome prepared by the preparation method according to any one of claims 2 to 5.
8. The whitening cosmetic of claim 7, wherein the whitening cosmetic comprises a whitening cream, a whitening emulsion, or a whitening spray.
9. The whitening cosmetic of claim 7 or 8, wherein the liposome is added in an amount of 1-3g in the whitening cosmetic.
CN202310048818.XA 2023-02-01 2023-02-01 Liposome, preparation method and application thereof Pending CN115778880A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116966106A (en) * 2023-08-16 2023-10-31 广州市澳莱化妆品有限公司 Liposome wrapper, preparation method and application

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103550135A (en) * 2013-11-08 2014-02-05 广州栋方日化有限公司 Whitening composition and cosmetic
CN104921973A (en) * 2014-03-19 2015-09-23 创庭化妆品科技(上海)有限公司 Liposome and preparation method thereof
CN107233238A (en) * 2016-03-23 2017-10-10 欧诗漫生物股份有限公司 A kind of cosmetics skin whitener and preparation method thereof
CN108210356A (en) * 2017-12-29 2018-06-29 上海乐宝日化股份有限公司 A kind of whitening emulsion and preparation method thereof
CN115040420A (en) * 2022-07-18 2022-09-13 云南贝泰妮生物科技集团股份有限公司 Pterostilbene liposome and preparation method thereof
CN115068384A (en) * 2022-07-08 2022-09-20 泉后(广州)生物科技研究院有限公司 Rapid whitening composition and application thereof
WO2022250542A1 (en) * 2021-05-28 2022-12-01 Mim Pharma As System-biology based pharmaceutical composition comprising a mixture of herb extracts as active ingredients, use of said composition as a medicament, particularly in the treatment of human coronavirus infections such as covid-19

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103550135A (en) * 2013-11-08 2014-02-05 广州栋方日化有限公司 Whitening composition and cosmetic
CN104921973A (en) * 2014-03-19 2015-09-23 创庭化妆品科技(上海)有限公司 Liposome and preparation method thereof
CN107233238A (en) * 2016-03-23 2017-10-10 欧诗漫生物股份有限公司 A kind of cosmetics skin whitener and preparation method thereof
CN108210356A (en) * 2017-12-29 2018-06-29 上海乐宝日化股份有限公司 A kind of whitening emulsion and preparation method thereof
WO2022250542A1 (en) * 2021-05-28 2022-12-01 Mim Pharma As System-biology based pharmaceutical composition comprising a mixture of herb extracts as active ingredients, use of said composition as a medicament, particularly in the treatment of human coronavirus infections such as covid-19
CN115068384A (en) * 2022-07-08 2022-09-20 泉后(广州)生物科技研究院有限公司 Rapid whitening composition and application thereof
CN115040420A (en) * 2022-07-18 2022-09-13 云南贝泰妮生物科技集团股份有限公司 Pterostilbene liposome and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116966106A (en) * 2023-08-16 2023-10-31 广州市澳莱化妆品有限公司 Liposome wrapper, preparation method and application
CN116966106B (en) * 2023-08-16 2024-04-16 广州市澳莱化妆品有限公司 Liposome wrapper, preparation method and application

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